journal
MENU ▼
Read by QxMD icon Read
search

Medical Microbiology and Immunology

journal
https://www.readbyqxmd.com/read/29147974/genotype-related-variations-in-proinflammatory-and-regulatory-cytokine-levels-in-treated-and-treatment-naive-hcv-infected-patients
#1
Rasoul Baharlou, Bizhan Romani, Seyed Jalal Kiani, Kaveh Sadeghi, Enayatollah Shadmand, Hadi Fazel, Farid Azizi Jalilian, Ebrahim Kord, Sajad Yaghoubi, Yousef Nikmanesh, Abbas Ahmadi Vasmehjani
Hepatitis C virus (HCV) modulates immune-related inflammatory responses to induce milder reactions leading to virus persistence. In this regard, the present study aimed to investigate the link between the HCV genotypes and the proinflammatory and regulatory cytokine levels. Ninety patients with hepatitis C infection (68 treatment-naive and 22 treated patients) and 76 healthy blood donors were studied. The serum levels of IFN-γ, IL-10, IL-17A, and IL-21 were measured by ELISA in the patients and healthy controls...
November 17, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/29119253/higher-expression-of-inhibitory-cd158b-and-cd158e-nk-cell-receptor-and-age-predicts-treatment-response-in-children-with-chronic-hepatitis-c
#2
Anna Mania, Mariusz Kaczmarek, Paweł Kemnitz, Katarzyna Mazur-Melewska, Magdalena Figlerowicz, Jan Sikora, Wojciech Służewski, Jan Żeromski
Treatment with pegylated interferon-α and ribavirin (PEG-IFN/RBV) is the only choice for chronic hepatitis C (CHC) in children. Natural killer (NK) cells were described to play a vital role in CHC. The aim of this study was to analyze the expression of peripheral blood NK cell receptors in their relation to PEG-IFN/RBV treatment response. Study included 26 children with CHC-13 boys, age range 13.42 ± 3.28 years. Blood for biochemical, virological and cytometric testing was taken for evaluation prior to the antiviral treatment...
November 8, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/29081001/antimicrobial-and-antibiofilm-activity-of-curcumin-silver-nanoparticles-with-improved-stability-and-selective-toxicity-to-bacteria-over-mammalian-cells
#3
Swati Jaiswal, Prashant Mishra
Antibiotic resistance has necessitated search for new antibacterials for combating threat of pathogenic bacteria. Though chemically synthesized silver nanoparticles are a well-known antimicrobial agent, they are toxic to human cells at higher concentrations. Hence in the present study, curcumin-silver nanoparticles (Cur-AgNPs) of size 25-35 nm, were synthesized using curcumin, a phytochemical. These nanoparticles were effective against both Gram positive and Gram-negative bacteria and were less toxic to human keratinocytes...
October 28, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/29043433/mcpip1-inhibits-coxsackievirus-b3-replication-by-targeting-viral-rna-and-negatively-regulates-virus-induced-inflammation
#4
Min Li, Kepeng Yan, Lin Wei, Yang Yang, Qian Qian, Wei Xu
Monocyte chemotactic protein-induced protein 1(MCPIP1) is identified as an important inflammatory regulator during immune response. MCPIP1 possesses antiviral activities against several viruses, such as Japanese encephalitis. However, its role on Coxsackievirus B3 (CVB3) infection, a positive-stranded RNA virus, has not been addressed. Here, we reported that MCPIP1 was up-regulated in cardiomyocytes by CVB3 infection and in hearts and pancreas of infected mice. Then we found that overexpression of MCPIP1 inhibited CVB3 replication and knockdown of it promoted virus replication...
October 17, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/29032460/the-impact-of-kir-hla-genotype-on-hepatitis-b-virus-clearance-in-iranian-infected-individuals
#5
Alireza Shah-Hosseini, Mohammad Jafari, Asadollah Mohammadi, Roozbeh Sanaei, Seyed Moayed Alavian, Amin Doosti-Irani, Mehrnoush Nooradeh Keykavousi, Nader Tajik
Killer cell immunoglobulin like receptors (KIRs) have a principal role in regulating the effector functions of NK cells, particularly in viral infections. The major ligands for KIRs are human leukocyte antigen (HLA) class I molecules. The aim of this study is to investigate the possible association of KIR genes, their known HLA ligands and compound KIR-HLA genotypes with hepatitis B virus (HBV) infection. Our study group consisted of 202 Iranian HBV-infected patients (52 spontaneously recovered, 50 asymptomatic carriers, 50 chronic sufferers and 50with liver cirrhosis) and 100 ethnic-matched healthy control subjects...
December 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28965239/burkholderia-cepacia-complex-in-cystic-fibrosis-in-a-brazilian-reference-center
#6
Priscila Dentini, Fernando Augusto Lima Marson, Luciana Cardoso Bonadia, Carmen Sílvia Bertuzzo, Antônio Fernando Ribeiro, Carlos Emílio Levy, José Dirceu Ribeiro
The Burkholderia cepacia complex (BCC) can cause a severe decline in lung function in cystic fibrosis (CF). Our objective was to determine the BCC prevalence and to evaluate its clinical impact on CF. Clinical and laboratory variables were determined for CF patients with BCC (Group-A = 50 patients) and without BCC (Group-B = 134 patients). The microorganisms were identified by biochemical tests, the Vitek2(®)Compact test, recA-PCR and recA-nested-PCR with species-specific primers and DNA sequencing. The patients were evaluated by the Shwachman-Kulczycki score (SKCS), Bhalla score (BS), spirometry and body mass index (BMI)...
December 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28884293/human-parainfluenza-virus-type-2-polymerase-complex-recognizes-leader-promoters-of-other-species-belonging-to-the-genus-rubulavirus
#7
Yusuke Matsumoto, Keisuke Ohta, Machiko Nishio
Leader sequence, located at the 3' terminus of paramyxovirus genomes, determines the degree of viral transcription and replication. The essential nucleotides in the leader sequence that influence viral propagation, however, have not been investigated in detail. In the present study, we show that polymerase complex of human parainfluenza virus type 2 (hPIV2) uses a luciferase-encoding hPIV2 mini-genome possessing the leader sequence from other closely related viruses as a template. Furthermore, we demonstrate that although hPIV2 polymerase complex can recognize the leader sequence of hPIV4B, mumps virus (MuV) and PIV5 as well as Newcastle disease virus (NDV), it cannot recognize measles virus, hPIV1, Sendai virus (SeV) or hPIV3...
December 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28875310/simultaneous-analysis-of-multiple-t-helper-subsets-in-leprosy-reveals-distinct-patterns-of-th1-th2-th17-and-tregs-markers-expression-in-clinical-forms-and-reactional-events
#8
Michelle de Campos Soriani Azevedo, Heloisa Marques, Larissa Sarri Binelli, Mariana Silva Vieira Malange, Amanda Carreira Devides, Eliane Aparecida Silva, Luciana Raquel Vincenzi Fachin, Cassio Cesar Ghidella, Cleverson Teixeira Soares, Gustavo Pompermaier Garlet, Patrícia Sammarco Rosa, Andrea de Farias Fernandes Belone, Ana Paula Favaro Trombone
Leprosy is a chronic infectious disease caused by Mycobacterium leprae. Previous studies have demonstrated that the difference among clinical forms of leprosy can be associated with the immune response of patients, mainly by T helper (Th) and regulatory T cells (Tregs). Then, aiming at clarifying the immune response, the expression of cytokines related to Th1, Th2, Th17 and Tregs profiles were evaluated by qPCR in 87 skin biopsies from leprosy patients. Additionally, cytokines and anti-PGL-1 antibodies were determined in serum by ELISA...
December 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28864951/time-course-of-cellular-hiv-dna-and-low-level-hiv-viremia-in-hiv-hcv%C3%A2-co-infected-patients-whose-hcv-infection-had-been-successfully-treated-with-directly-acting-antivirals
#9
Saverio G Parisi, Samantha Andreis, Monica Basso, Silvia Cavinato, Renzo Scaggiante, Marzia Franzetti, Massimo Andreoni, Giorgio Palù, Anna Maria Cattelan
This longitudinal study described cellular HIV-DNA changes and their correlation with HIV low-level plasma viremia (LLV) in HIV-HCV co-infected patients on successful antiretroviral and anti-HCV therapy by treatment with direct-acting antivirals (DAA). Thirty-nine patients were examined prior to the start of DAA (T0), after week 12 (T1) and 24 weeks (T2) of anti-HCV therapy. Cellular PBMC HIV-DNA was analysed as an absolute value and as the percentage of increase or decrease from T0 to T2. Patients were classified as having undetectable plasma HIV viraemia (UV) or LLV in the year before the start of anti-HCV treatment and within the T0-T2 study period...
December 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28770333/immune-response-against-rickettsiae-lessons-from-murine-infection-models
#10
REVIEW
Anke Osterloh
Rickettsiae are small intracellular bacteria that can cause life-threatening febrile diseases. Rickettsioses occur worldwide with increasing incidence. Therefore, a vaccine is highly desired. A prerequisite for the development of a vaccine is the knowledge of the immune response against these bacteria, in particular protective immunity. In recent years murine models of rickettsial infections have been established, and the study of immune response against rickettsiae in mice provided many new insights into protective and pathological immune reactions...
December 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28852878/compensating-for-cross-reactions-using-avidity-and-computation-in-a-suspension-multiplex-immunoassay-for-serotyping-of-zika-versus-other-flavivirus-infections
#11
Bengt Rönnberg, Åke Gustafsson, Olli Vapalahti, Petra Emmerich, Åke Lundkvist, Jonas Schmidt-Chanasit, Jonas Blomberg
The recent spread of Zika virus (ZIKV) in the Americas and Asia necessitates an increased preparedness for improved maternal and perinatal health and blood safety. However, serological cross-reactions, especially to Dengue virus (DENV), complicate ZIKV antibody serodiagnosis. A novel "pan-Flavi" suspension multiplex immunoassay (PFSMIA) using 25 antigens, whole virus (WV), non-structural protein 1 (NS1), and envelope (E) proteins, from 7 zoonotic flaviviruses for specific detection of ZIKV and DENV IgM and IgG was developed...
October 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28815296/autoantibodies-against-rods-and-rings-related-impdh2-in-hepatitis-c-genotype-1-and-daa-therapy-in-a-real-life-cohort
#12
Werner Dammermann, Susanne Polywka, Inga Dettmann, Swantje Mindorf, Lars Komorowski, Malte Wehmeyer, Julian Schulze Zur Wiesch, Winfried Stöcker, Stefan Lüth
Autoantibodies against inosine-5'-monophosphate-dehydrogenase-2 (IMPDH2; "rods and rings" pattern) develop in chronic hepatitis C (CHC) patients under treatment with peg-interferon (IFN) and ribavirin (RBV), an inhibitor of IMPDH2. We investigated the influence of the alternative therapy with direct-acting antivirals (DAA)/ribavirin on anti-IMPDH2 autoantibody generation and the use of anti-IMPDH2 development as a marker for therapy outcome (sustained virologic response, SVR). We analyzed a "real life" cohort of 104 unselected CHC genotype 1 (GT1) patients treated with IFN/first-generation DAA/RBV prospectively compared to a historic cohort of 59 IFN/RBV-treated CHC GT1 patients...
October 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28776194/a-cross-sectional-study-among-polish-hunters-seroprevalence-of-hepatitis-e-and-the-analysis-of-factors-contributing-to-hev-infections
#13
Anna Baumann-Popczyk, Bartłomiej Popczyk, Elżbieta Gołąb, Wioletta Rożej-Bielicka, Małgorzata Sadkowska-Todys
Hepatitis E virus (HEV) is known as zoonotic agent. The main reservoirs of HEV in Europe are pigs, wild boars, and deer. Hunting activity is considered to be a risk factor for HEV infection. We conducted a cross-sectional study among 1021 Polish hunters. To understand socio-demographic characteristics of this population and to gather information on potential exposures, all participants completed a questionnaire. Commercial immunoassays were employed to estimate seroprevalence anti-HEV. Samples with confirmed positive result of anti-HEV IgM were examined for HEV RNA...
October 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28733760/intrauterine-therapy-of-cytomegalovirus-infection-with-valganciclovir-review-of-the-literature
#14
REVIEW
Vera Seidel, Cornelia Feiterna-Sperling, Jan-Peter Siedentopf, Jörg Hofmann, Wolfgang Henrich, Christoph Bührer, Katharina Weizsäcker
Congenital cytomegalovirus (CMV) infection is the leading cause for sensorineural hearing loss and mental retardation in children without genetic diseases worldwide. There is little evidence guiding therapeutic strategies during pregnancy when intrauterine fetal CMV infection is confirmed. We provide a systematic review of the use of ganciclovir (GCV) or VGCV during pregnancy discussing safety of its use for mother and fetus and describe two cases of intrauterine therapy of fetal CMV infection with valganciclovir (VGCV)...
October 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28730548/orthopedic-infections-caused-by-obligatory-anaerobic-gram-negative-rods-report-of-two-cases
#15
Marta Kierzkowska, Piotr Pedzisz, Ireneusz Babiak, Jakub Janowicz, Mateusz Kulig, Anna Majewska, Anna Sawicka-Grzelak, Grazyna Mlynarczyk
Anaerobic bone and joint infections are uncommon, although the number of anaerobic infections is presumably underestimated because of difficulties with isolation and identification of obligate anaerobes. This study describes two cases of complicated Bacteroides fragilis peri-implant infection of the lumbar spine, infection of the hip and osteomyelitis. Bacteria were identified with the use of a mass spectrometer, VITEK MS system. Drug susceptibility was performed with the use of E-test. The EUCAST breakpoints were used for interpretation with B...
October 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28702856/dynamics-of-torque-teno-virus-viremia-could-predict-risk-of-complications-after-allogeneic-hematopoietic-stem-cell-transplantation
#16
Ramona Gilles, Marco Herling, Udo Holtick, Eva Heger, Sabine Awerkiew, Irina Fish, Konstantin Höller, Saleta Sierra, Elena Knops, Rolf Kaiser, Christof Scheid, Veronica Di Cristanziano
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is an established treatment option for several hematological diseases. However, the first year post-transplantation is often complicated by infections and graft-versus-host disease (GVHD). Improvements in immunological monitoring could reduce such post-transplant complications. Torque Teno virus (TTV), a chronically persisting DNA virus, is reported to be a marker for immune function in immunocompromised patients. In the present study, the TTV kinetics were analyzed to investigate the potential role of TTV viremia as immune-competence read-out after allo-HSCT...
October 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28474248/a-potential-role-for-staphylococcal-and-streptococcal-superantigens-in-driving-skewing-of-tcr-v%C3%AE-subsets-in-tonsillar-hyperplasia
#17
Fiona J Radcliff, Fiona Clow, Murali Mahadevan, James Johnston, Thomas Proft, Richard G Douglas, John D Fraser
The TCR Vβ repertoire from patients with recurrent tonsillitis and/or tonsillar hyperplasia was examined to determine whether the TCR Vβ composition is suggestive of local superantigen activity and if so, whether it is associated with the presence of superantigen producing bacteria. Tonsil specimens were cultured aerobically to allow identification and isolation of the bacterial pathogens Staphylococcus aureus and Group A Streptococcus. TCR Vβ subset analysis of tonsil leucocytes was performed by flow cytometry...
August 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28466382/assessment-of-htlv-1-proviral-load-lat-bim-c-fos-and-rad51-gene-expression-in-adult-t-cell-leukemia-lymphoma
#18
Samaneh Ramezani, Abbas Shirdel, Houshang Rafatpanah, Mohammad Mehdi Akbarin, Hanieh Tarokhian, Hossein Rahimi, Alireza Bari, Hamid Reza Jahantigh, Seyed Abdolrahim Rezaee
Adult T cell leukemia/lymphoma (ATLL) is a life-threatening malignancy of HTLV-1 infected Th lymphocytes. In the present study host-virus interactions were investigated by assessment of HTLV-1 proviral load (PVL) and host gene expression. A cross-sectional study was carried out on 18 ATLL, 10 HAM/TSP patients and 18 HTLV-1 asymptomatic carriers (ACs). DNA and mRNA of the peripheral blood mononuclear cells were extracted for PVL and LAT, BIM, c-FOS and RAD51 gene expression measurement using qRT-PCR. The mean PVL in ATLL patients was 11,430 ± 3770 copies/10(4) which was statistically higher than ACs, 530 ± 119 copies/10(4), (p < 0...
August 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28466381/tetherin-antagonism-by-v-proteins-is-a-common-trait-among-the-genus-rubulavirus
#19
Keisuke Ohta, Yusuke Matsumoto, Morihiro Ito, Machiko Nishio
Tetherin (BST-2/CD317/HM1.24) is an anti-viral factor that restricts the budding of several enveloped viruses. Most of these viruses have evolved to encode tetherin antagonists. Our previous study demonstrated that the growth of human parainfluenza virus type 2 (hPIV-2), a member of the genus Rubulavirus in the family Paramyxoviridae, was inhibited by tetherin, and its V protein decreases the amount of cell surface tetherin by the interaction. In the present study, we investigated whether tetherin inhibits the growth of other rubulaviruses including PIV-5, mumps virus (MuV), simian virus 41, and hPIV-4, and whether their V proteins act as tetherin antagonists...
August 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28455649/human-parainfluenza-virus-type-2%C3%A2-v-protein-inhibits-induction-of-tetherin
#20
Keisuke Ohta, Yusuke Matsumoto, Natsuko Yumine, Machiko Nishio
Tetherin is an anti-viral factor that restricts viral budding through tethering virions to the cell surface. The human parainfluenza virus type 2 (hPIV-2) V protein decreases cell surface tetherin in HeLa cells, which constitutively express endogenous tetherin. However, the role of the hPIV-2 V protein in tetherin induction remains unclear. Here, we examined whether hPIV-2 infection itself induces tetherin in HEK293 cells that have no basal expression of tetherin. Unlike influenza A virus (IAV) infection, hPIV-2 infection induced neither tetherin mRNA nor protein expression...
August 2017: Medical Microbiology and Immunology
journal
journal
22727
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"