journal
MENU ▼
Read by QxMD icon Read
search

International Journal for Parasitology

journal
https://www.readbyqxmd.com/read/28739251/evidence-for-an-important-role-of-host-micrornas-in-regulating-hepatic-fibrosis-in-humans-infected-with-schistosoma-japonicum
#1
Sandrine Cabantous, Xunya Hou, Laurence Louis, Hongbin He, Odette Mariani, Xavier Sastre, Martine Daujat-Chavanieu, Yuesheng Li, Alain Dessein
MicroRNAs (miRNAs) are short, non-coding RNAs that repress the translation of target gene transcripts. They have been implicated in various activities such as cell proliferation, survival, differentiation, migration and metabolism. We report here the first known miRNome and transcriptome analysis of human livers displaying advanced fibrosis due to Schistosoma japonicum infection. We present evidence that hsa-miR-150-5p, hsa-miR-10a-5p, hsa-miR-199a-3p, hsa-miR-4521, hsa-miR-222/221, hsa-miR-663b and hsa-miR-143-3p (associated without correction) play an important role in hepatic fibrosis by acting on metabolism, organization of the extracellular matrix proteins, lipid mobilization and limitation of oxidative damage stress...
July 21, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28694188/testing-the-enemy-release-hypothesis-abundance-and-distribution-patterns-of-helminth-communities-in-grey-mullets-teleostei-mugilidae-reveal-the-success-of-invasive-species
#2
Volodimir Sarabeev, Juan Antonio Balbuena, Serge Morand
The abundance and aggregation patterns of helminth communities of two grey mullet hosts, Liza haematocheilus and Mugil cephalus, were studied across 14 localities in Atlantic and Pacific marine areas. The analysis matched parasite communities of (i) L. haematocheilus across its native and introduced populations (Sea of Japan and Sea of Azov, respectively) and (ii) the introduced population of L. haematocheilus with native populations of M. cephalus (Mediterranean, Azov-Black and Japan Seas). The total mean abundance (TMA), as a feature of the infection level in helminth communities, and slope b of the Taylor's power law, as a measure of parasite aggregation at the infra and component-community levels, were estimated and compared between host species and localities using ANOVA...
July 8, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28694187/a-more-sensitive-efficient-and-iso-17025-validated-magnetic-capture-real-time-pcr-method-for-the-detection-of-archetypal-toxoplasma-gondii-strains-in-meat
#3
Ignacio Gisbert Algaba, Manon Geerts, Malgorzata Jennes, Wim Coucke, Marieke Opsteegh, Eric Cox, Pierre Dorny, Katelijne Dierick, Stéphane De Craeye
Toxoplasma gondii is a globally prevalent, zoonotic parasite of major importance to public health. Various indirect and direct methods can be used for the diagnosis of toxoplasmosis. Whereas serological tests are useful to prove contact with the parasite has occurred, the actual presence of the parasite in the tissues of a seropositive animal is not demonstrated. For this, a bioassay is still the reference method. As an alternative, various PCR methods have been developed, but due to the limited amount of sample that can be tested, combined with a low tissue cyst density, those have proved to be insufficiently sensitive...
July 8, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28668326/influences-of-climate-change-on-the-potential-distribution-of-lutzomyia-longipalpis-sensu-lato-psychodidae-phlebotominae
#4
A Townsend Peterson, Lindsay P Campbell, David A Moo-Llanes, Bruno Travi, Camila González, María Cristina Ferro, Gabriel Eduardo Melim Ferreira, Sinval P Brandão-Filho, Elisa Cupolillo, Janine Ramsey, Andreia Mauruto Chernaki Leffer, Angélica Pech-May, Jeffrey J Shaw
This study explores the present day distribution of Lutzomyia longipalpis in relation to climate, and transfers the knowledge gained to likely future climatic conditions to predict changes in the species' potential distribution. We used ecological niche models calibrated based on occurrences of the species complex from across its known geographic range. Anticipated distributional changes varied by region, from stability to expansion or decline. Overall, models indicated no significant north-south expansion beyond present boundaries...
June 29, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28668325/igg-opsonization-of-merozoites-multiple-immune-mechanisms-for-malaria-vaccine-development
#5
REVIEW
Danika L Hill, Louis Schofield, Danny W Wilson
Global eradication of the human-infecting malaria parasite Plasmodium falciparum, the major cause of malaria mortality, is unlikely to be achieved without an effective vaccine. However, our limited understanding of how protective immune responses target malaria parasites in humans, and how to best elicit these immune responses through vaccination, has hampered vaccine development. The red blood cell invading stage of the parasite lifecycle (merozoite) displays antigens that are attractive vaccine candidates as they are accessible to antibodies and raise high antibody titres in naturally immune individuals...
June 29, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28668324/population-genetic-analysis-informs-the-invasion-history-of-the-emerging-trematode-dicrocoelium-dendriticum-into-canada
#6
Bradley J van Paridon, Douglas D Colwell, Cameron P Goater, John S Gilleard
Parasite distributions are constantly changing due to climate change, local and global movement of animals and humans, as well as land use and habitat change. The trematode Dicrocoelium dendriticum is a relatively recent invader of Canada, being first reported in eastern Canada in the 1930s and western Canada in the 1970s. However, historical records are scarce and its emergence is poorly understood. The establishment of this parasite in Canada provides an interesting opportunity to explore the use of population genetic approaches to help elucidate the invasion history of a relatively recently established helminth parasite...
June 29, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28668323/cestode-parasites-release-extracellular-vesicles-with-micrornas-and-immunodiagnostic-protein-cargo
#7
María Eugenia Ancarola, Antonio Marcilla, Michaela Herz, Natalia Macchiaroli, Matías Pérez, Sebastián Asurmendi, Klaus Brehm, Carolina Poncini, Mara Rosenzvit, Marcela Cucher
Intercellular communication is crucial in multiple aspects of cell biology. This interaction can be mediated by several mechanisms including extracellular vesicle (EV) transfer. EV secretion by parasites has been reported in protozoans, trematodes and nematodes. Here we report that this mechanism is present in three different species of cestodes, Taenia crassiceps, Mesocestoides corti and Echinococcus multilocularis. To confirm this we determined, in vitro, the presence of EVs in culture supernatants by transmission electron microscopy...
June 29, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28606698/comparative-analyses-of-whole-genome-sequences-of-leishmania-infantum-isolates-from-humans-and-dogs-in-northeastern-brazil
#8
D G Teixeira, G R G Monteiro, D R A Martins, M Z Fernandes, V Macedo-Silva, M Ansaldi, P R P Nascimento, M A Kurtz, J A Streit, M F F M Ximenes, R D Pearson, A Miles, J M Blackwell, M E Wilson, A Kitchen, J E Donelson, J P M S Lima, S M B Jeronimo
The genomic sequences of 20 Leishmania infantum isolates collected in northeastern Brazil were compared with each other and with the available genomic sequences of 29 L. infantum/donovani isolates from Nepal and Turkey. The Brazilian isolates were obtained in the early 1990s or since 2009 from patients with visceral or non-ulcerating cutaneous leishmaniasis, asymptomatic humans, or dogs with visceral leishmaniasis. Two isolates were from the blood and bone marrow of the same visceral leishmaniasis patient. All 20 genomic sequences display 99...
June 10, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28606697/whipworm-kinomes-reflect-a-unique-biology-and-adaptation-to-the-host-animal
#9
Andreas J Stroehlein, Neil D Young, Pasi K Korhonen, Bill C H Chang, Peter Nejsum, Edoardo Pozio, Giuseppe La Rosa, Paul W Sternberg, Robin B Gasser
Roundworms belong to a diverse phylum (Nematoda) which is comprised of many parasitic species including whipworms (genus Trichuris). These worms have adapted to a biological niche within the host and exhibit unique morphological characteristics compared with other nematodes. Although these adaptations are known, the underlying molecular mechanisms remain elusive. The availability of genomes and transcriptomes of some whipworms now enables detailed studies of their molecular biology. Here, we defined and curated the full complement of an important class of enzymes, the protein kinases (kinomes) of two species of Trichuris, using an advanced and integrated bioinformatic pipeline...
June 10, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28606696/targeted-gene-knockdown-validates-the-essential-role-of-lactate-dehydrogenase-in-cryptosporidium-parvum
#10
William H Witola, Xuejin Zhang, Chi Yong Kim
Cryptosporidium parvum is a zoonotic protozoan that can cause a life-threatening gastrointestinal syndrome in children and in immunocompromised adults. Currently, the only approved drug for treatment of Cryptosporidium infections in humans is nitazoxanide, but it is not effective in immunocompromised individuals or in children with malnutrition. This is compounded by the lack of genetic methods for studying and validating potential drug targets in the parasite. Therefore, in this study, we endeavoured to adapt the use of a phosphorodiamidate morpholino oligomer (morpholino) antisense approach to develop a targeted gene knockdown assay for use in C...
June 9, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28577882/heritable-genetic-transformation-of-strongyloides-stercoralis-by-microinjection-of-plasmid-dna-constructs-into-the-male-germline
#11
Hongguang Shao, Xinshe Li, James B Lok
Heretofore, transgenesis in the parasitic nematode genus Strongyloides has relied on microinjecting transgene constructs into gonadal syncytia of free-living females. We now report transgenesis in Strongyloides stercoralis by microinjecting constructs into the syncytial testes of free-living males. Crosses of individual males microinjected with a construct encoding GFP with cohorts of 12 non-injected females produced a mean of 7.28±2.09 transgenic progeny. Progeny of males and females microinjected with distinct reporter constructs comprised 2...
June 1, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28577881/gene-enriched-draft-genome-of-the-cattle-tick-rhipicephalus-microplus-assembly-by-the-hybrid-pacific-biosciences-illumina-approach-enabled-analysis-of-the-highly-repetitive-genome
#12
Roberto A Barrero, Felix D Guerrero, Michael Black, John McCooke, Brett Chapman, Faye Schilkey, Adalberto A Pérez de León, Robert J Miller, Sara Bruns, Jason Dobry, Galina Mikhaylenko, Keith Stormo, Callum Bell, Quanzhou Tao, Robert Bogden, Paula M Moolhuijzen, Adam Hunter, Matthew I Bellgard
The genome of the cattle tick Rhipicephalus microplus, an ectoparasite with global distribution, is estimated to be 7.1Gbp in length and consists of approximately 70% repetitive DNA. We report the draft assembly of a tick genome that utilized a hybrid sequencing and assembly approach to capture the repetitive fractions of the genome. Our hybrid approach produced an assembly consisting of 2.0Gbp represented in 195,170 scaffolds with a N50 of 60,284bp. The Rmi v2.0 assembly is 51.46% repetitive with a large fraction of unclassified repeats, short interspersed elements, long interspersed elements and long terminal repeats...
June 1, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28528874/animal-health-and-greenhouse-gas-intensity-the-paradox-of-periparturient-parasitism
#13
J G M Houdijk, B J Tolkamp, J A Rooke, M R Hutchings
Here we provide the first known direct measurements of pathogen challenge impacts on greenhouse gas production, yield and intensity. Twin-rearing ewes were ad libitum fed pelleted lucerne from day -32 to 36 (day 0 is parturition), and repeatedly infected with 10,000 Teladorsagia circumcincta infective larvae (n=16), or sham-dosed with water (n=16). A third group of 16 ewes were fed at 80% of uninfected ewes' feed intake during lactation. Methane emissions were measured in respiration chambers (day 30-36) whilst total tract apparent nutrient digestibility around day 28 informed calculated manure methane and nitrous oxide emissions estimates...
May 18, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28526608/microrna-analysis-of-taenia-crassiceps-cysticerci-under-praziquantel-treatment-and-genome-wide-identification-of-taenia-solium-mirnas
#14
Matías Gastón Pérez, Natalia Macchiaroli, Gabriel Lichtenstein, Gabriela Conti, Sebastián Asurmendi, Diego Humberto Milone, Georgina Stegmayer, Laura Kamenetzky, Marcela Cucher, Mara Cecilia Rosenzvit
MicroRNAs (miRNAs) are small non-coding RNAs that have emerged as important regulators of gene expression and perform critical functions in development and disease. In spite of the increased interest in miRNAs from helminth parasites, no information is available on miRNAs from Taenia solium, the causative agent of cysticercosis, a neglected disease affecting millions of people worldwide. Here we performed a comprehensive analysis of miRNAs from Taenia crassiceps, a laboratory model for T. solium studies, and identified miRNAs in the T...
May 17, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28526607/the-merozoite-specific-protein-tggra11b-identified-as-a-component-of-the-toxoplasma-gondii-parasitophorous-vacuole-in-a-tachyzoite-expression-model
#15
Chandra Ramakrishnan, Robert A Walker, Ramon M Eichenberger, Adrian B Hehl, Nicholas C Smith
The apicomplexan, Toxoplasma gondii, infects all warm-blooded animals as intermediate hosts but only felids as definitive hosts. Dense granule proteins are critical for the survival of Toxoplasma within host cells but, whilst these proteins have been studied intensively in tachyzoites, little is known about their expression in the coccidian stages in the cat intestine. Transcriptomic profiling indicates that two putative dense granule proteins, TgGRA11A and TgGRA11B, are expressed uniquely in merozoites. Immunofluorescent microscopy of Toxoplasma-infected cat intestine and tachyzoites engineered to express TgGRA11B, reveals that it is a dense granule protein that traffics into the parasitophorous vacuole and its membrane...
May 17, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28526606/identification-of-emsox2-a-member-of-the-sox-family-of-transcription-factors-as-a-potential-regulator-of-echinococcus-multilocularis-germinative-cells
#16
Zhe Cheng, Fan Liu, Mengya Dai, Jianjian Wu, Xiu Li, Xinrui Guo, Huimin Tian, Zhijie Heng, Ying Lu, Xiaoli Chai, Yanhai Wang
Larvae of the tapeworm Echinococcus multilocularis cause alveolar echinococcosis (AE), one of the most lethal helminthic infections in humans. The germinative cells, a population of stem cell-like cells, are considered to drive the continuous growth of the metacestodes within the host. The mechanisms and relative molecules controlling the behavior of germinative cells are poorly understood. Sox transcription factors play important roles in maintenance and regulation of stem/progenitor cells. We here describe the identification of a Sox family member in E...
May 17, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28526605/microevolutionary-response-of-a-gut-nematode-to-intestinal-inflammation
#17
Cédric Lippens, Bruno Faivre, Gabriele Sorci
Parasitic helminths interfere with the immune responses of their hosts to establish long-lasting, chronic infections. While favorable to the parasite, the capacity to dampen the immune response can also provide a benefit to the host in terms of reduced risk of immune disorders and immunopathology. The immunomodulatory role of nematodes has been exploited in clinical trials to treat a number of inflammatory and immune diseases. However, how parasites adapt to an inflammatory environment remains a poorly explored question...
May 17, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28495122/next-generation-sequencing-uncovers-within-host-differences-in-the-genetic-diversity-of-cryptosporidium-gp60-subtypes
#18
Alireza Zahedi, Alexander William Gofton, Fuchun Jian, Andrea Paparini, Charlotte Oskam, Andrew Ball, Ian Robertson, Una Ryan
The extent of within-host genetic diversity of parasites has implications for our understanding of the epidemiology, disease severity and evolution of parasite virulence. As with many other species, our understanding of the within-host diversity of the enteric parasite Cryptosporidium is changing. The present study compared Sanger and Next Generation Sequencing of glycoprotein 60 (gp60) amplicons from Cryptosporidium hominis (n=11), Cryptosporidium parvum (n=22) and Cryptosporidium cuniculus (n=8) DNA samples from Australia and China...
May 8, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28347664/venestatin-a-ca-binding-protein-from-the-parasitic-nematode-strongyloides-venezuelensis-is-involved-in-the-larval-migration-process
#19
Daigo Tsubokawa, Takeshi Hatta, Taisei Kikuchi, Hiroki Maeda, Fusako Mikami, M Abdul Alim, Haruhiko Maruyama, Naotoshi Tsuji
The secretory EF-hand Ca(++)-binding proteins act as calcium signaling molecules for control of cell functions, but those proteins from parasitic helminths are poorly understood. Here, we have identified and characterized an EF-hand Ca(++)-binding protein from the rodent nematode, Strongyloides venezuelensis, termed 'venestatin', which is highly conserved in Strongyloides spp. Canonical two EF-hand domains and a signal peptide are present in venestatin. A gel mobility shift assay and Ruthenium red staining indicated that the recombinant venestatin possesses binding ability with Ca(++) ions...
July 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28344097/whence-river-blindness-the-domestication-of-mammals-and-host-parasite-co-evolution-in-the-nematode-genus-onchocerca
#20
Emilie Lefoulon, Alessio Giannelli, Benjamin L Makepeace, Yasen Mutafchiev, Simon Townson, Shigehiko Uni, Guilherme G Verocai, Domenico Otranto, Coralie Martin
The genus Onchocerca includes 34 described species and represents one of the largest genera of the filarial nematodes within the family Onchocercidae. Representative members of this genus are mainly parasites of ungulates, with some exceptions such as Onchocerca lupi and Onchocerca volvulus, infecting carnivores and/or humans. For a long time, the evolutionary relationships amongst onchocercids remained poorly studied, as the systematics of this genus was impaired by the high morphological variability of species included in the taxon...
July 2017: International Journal for Parasitology
journal
journal
22722
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"