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Laboratory Medicine

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https://www.readbyqxmd.com/read/28057801/a-simple-pcr-rflp-method-for-genotyping-of-ifnl4-rs368234815-polymorphism-in-patients-with-chronic-hepatitis-c
#1
Mohammad Pouryasin, Heidar Sharafi, Bita Behnava, Seyed Moayed Alavian, Maryam Keshvari, Ali Pouryasin
BACKGROUND: The IFNL4 rs368234815 polymorphism plays a prominent role in spontaneous and treatment-induced clearance of hepatitis C virus (HCV) infection. This study aimed to develop a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for assessment of rs368234815 polymorphism. METHODS: We genotyped the rs368234815 polymorphism in 87 patients with chronic HCV by PCR sequencing and PCR-RFLP methods, simultaneously. RESULTS: Genotyping of IFNL4 rs368234815 via PCR-RFLP was concordant with PCR sequencing in all 87 individuals (100%)...
January 4, 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/28039379/green-neutrophilic-inclusions-are-frequently-associated-with-liver-injury-and-may-portend-short-term-mortality-in-critically-ill-patients
#2
Elizabeth L Courville, Susan Crisman, Michael A Linden, Sophia Yohe
BACKGROUND: Few studies have described an association among acute liver disease, high short-term mortality, and blue-green refractile neutrophilic inclusions on blood smears. OBJECTIVE: To describe our experience with 13 patients and compare our data to those from other cases reported in the literature regarding the interplay of acute liver disease, high short-term mortality, and blue-green refractile neutrophilic inclusions on blood smears. METHODS: We performed a retrospective review of blood smears, patient medical information, and outcomes for our 13 studied patients...
December 29, 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/28039378/haemophilus-species-as-a-urinary-tract-pathogen
#3
L Karen Diedrich, Cora L Manby
Haemophilus species, as a urinary tract pathogen, is rarely encountered. Routine urine culture pathogens are well documented to include Enterobacteriaciae, Enterococcus spp., Staphylococcus spp., Pseudomonas spp., and Candida spp. Pathogens encountered rarely in this context may include Corynebacterium ureolyticus and nonfermenting gram negative bacilli. This case study involves the finding of Haemophilus influenzae in a urine culture performed on a 4-year-old Caucasian girl and Haemophilus parinfluenzae in a 60-year-old African American man with pyelonephritis...
December 29, 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/28039377/detection-of-serotype-distribution-and-drug-resistance-of-streptococcus-pneumoniae-isolated-from-pediatric-patients
#4
Junrong Wang, Fenqin Liu, Peiran Ao, Xianneng Li, Haixiao Zheng, Di Wu, Nina Zhang, Junping Yu, Junhui Yuan, Xiuying Wu
OBJECTIVE: To analyze the serotype distribution and drug resistance of Streptococcus pneumoniae isolated from pediatric patients in a Chinese hospital. METHODS: From October 2011 to May 2014, we collected 284 isolates (including 217 noninvasive and 67 invasive strains). We tested the antimicrobial susceptibility of these specimens using the Epsilomer test and disk diffusion method, and the expression of macrolide resistant genes ermB and mefA by polymerase chain reaction...
December 29, 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/28007780/ficin-treated-red-cells-help-identify-clinically-significant-alloantibodies-masked-as-reactions-of-undetermined-specificity-in-gel-microtubes
#5
Ben C Hill, Courtney A Hanna, Jill Adamski, Huy P Pham, Marisa B Marques, Lance A Williams
Non-specific antibodies or antibodies of undetermined significance (AUS) often pose problems for a blood bank technologist and physician. It is well known that antibodies can weaken and evanesce over time, thus eluding detection by routine blood bank techniques. Special enhancement techniques exist (eg, ficin treatment); however, they are often underutilized due to concerns over expense. Ficin is known to enhance reactivity caused by antibodies in the ABO, Rh, Kidd, Lewis, I, and P blood group systems, while destroying reactivity of antibodies in the Duffy, and MNS blood group systems...
December 22, 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27965275/gastric-strongyloidiasis-report-of-2-cases-and-brief-review-of-the-literature
#6
Rawia Mohamed, Mowafak M Hamodat, Mousa A Al-Abbadi
Strongyloides stercoralis is an intestinal nematode with a complicated lifecycle and auto-infection. In healthy individuals, this parasite usually does not cause any symptoms. But in immunocompromised patients, the symptoms vary from abdominal pain and discomfort to sepsis and death.Gastric infection and involvement by this nematode are rare and uncommon. Here we report 2 cases of Strongyloides stercoralis hyperinfection that were discovered in gastric biopsies in 2 immunocompromised patients. A gastric biopsy was taken for each patient and both showed cross sections of adult worms, eggs, and rhabditiform larvae of S...
December 13, 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27794527/the-challenges-of-daratumumab-in-transfusion-medicine
#7
REVIEW
Michael F Dizon
The field of transfusion medicine has evolved rapidly in recent years, but the central principle of transfusion is still simple, namely, the antigen-antibody interaction. Daratumumab (DARA), a monoclonal antibody (MoAb), was developed to treat relapsed/refractory multiple myeloma (RRMM). DARA works by targeting the CD38 portion of malignant cells; however, this drug attaches to the red blood cell (RBC) reagents used in blood banks, further complicating the antibody identification work-up. The AABB (formerly known as the American Association of Blood Banks) has issued a memorandum on how blood banks can effectively address panreactivity caused by DARA...
October 28, 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27794526/association-of-ucp2-866g-a-polymorphism-with-nonalcoholic-fatty-liver-disease-in-patients-from-north-west-of-iran
#8
Fatemeh Mohseni, Safar Farajnia, Mahdieh Abbasalizad Farhangi, Manouchehr Khoshbaten, Mohammad-Asghari Jafarabadi
OBJECTIVE: To investigate the association of uncoupling protein-2 (UCP2) -866G > A gene polymorphism (rs659366) with nonalcoholic fatty liver disease (NAFLD). METHODS: We performed a case-control study with a cohort of 75 patients with NAFLD (of Iranian ethnicity) and 76 healthy individuals of Iranian ethnicity. The UCP2 -866G > A polymorphism (rs659366) was determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)...
October 28, 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27771620/a-survey-of-knowledge-and-practices-of-transfusion-medicine-among-post-intern-doctors-in-specialized-hospital-in-sri-lanka
#9
Samantha Kumarage, Rahal Fernando, Lanka Gunasekara
BACKGROUND: Knowledge of transfusion medicine is the key element of better transfusion practices. This deficit observed at the blood bank end on daily basis exposing the patients for redundant risk. We assessed the knowledge of transfusion medicine among post intern doctors. OBJECTIVE: To assess the knowledge of transfusion medicine among post intern doctors in working in our hospital. METHODS: Self administrated questionnaire was used. 45 questions of transfusion medicine included in the questioner...
October 22, 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27708176/about-the-journal
#10
(no author information available yet)
No abstract text is available yet for this article.
November 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27708175/table-of-contents-toc
#11
(no author information available yet)
No abstract text is available yet for this article.
November 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27708174/cover
#12
(no author information available yet)
No abstract text is available yet for this article.
November 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27708173/iqcp-guideline-and-helpful-tools-for-implementation
#13
Linda C Bruno
This article will help laboratories understand what changed with external quality control testing, why it changed, who changed it, when the changes became effective, and how these changes affect clinical laboratories. The new Individualized Quality Control Plan (IQCP) option that became effective January 1, 2016, will be explained. Three other quality control changes that took effect January 1, 2016, and dramatically affect quality control requirements for antimicrobial susceptibility testing, identification test systems, and exempt culture media will also be explained...
November 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27708172/handling-big-data-in-modern-healthcare
#14
Hassan A Aziz
The constant growth of medical knowledge and the increases in specialization in clinical practice have created a significant need to share and access patient information with speed and efficiency. However, current technology is centered on processing data, rather than gathering information. To realize the potential of modern technology in improving patient health, merely collecting and storing data are insufficient: one must convert these data into information and knowledge. In this article, I present an overview of 2 recent advances in technology and their assimilation into the practice of medicine, in the attempt to make clinical data meaningful and then to learn from aggregated clinical data...
November 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27708171/erratum
#15
(no author information available yet)
No abstract text is available yet for this article.
November 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27708170/prevalence-and-clinical-utility-of-incidental-critical-values-resulting-from-critical-care-laboratory-testing
#16
Caleb V Grieme, Dena R Voss, Karin E Olson, Scott R Davis, Jeff Kulhavy, Matthew D Krasowski
OBJECTIVE: Panels of clinical laboratory testing may generate "incidental" critical values from unordered parameters. Existing regulations do not clearly delineate guidelines for handling incidental critical values. The objective of this study was to examine the patterns and clinical utility of incidental critical values at 2 critical care laboratories within an academic medical center. METHODS: In this retrospective study, the electronic health record and laboratory information system were reviewed for incidental critical results obtained from blood gas analyzer analysis of whole blood specimens between November 2010 and August 2014...
November 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27708169/the-need-for-education-in-molecular-immunohematology-a-survey-of-specialists-in-blood-banking
#17
Stephanie N Kinney, Jayanna Slayten, Dan A Waxman
BACKGROUND: Within transfusion medicine, the education of molecular technologies lacks standardization. OBJECTIVE: To address this problem, we surveyed specialist in blood bank technology (SBBT) programs, immunohematology reference laboratories, and SBBT graduates to define its current state. METHODS: An anonymous online survey (SurveyMonkey) was emailed to the 15 American Association of Blood Banks (AABB) SBBT programs, 59 AABB IRLs, and 82 SBBT graduates...
November 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27708168/dna-methylation-of-the-htert-gene-in-breast-cancer-revisited-diagnostic-and-clinical-implications
#18
Shahla Masood, Ehab El-Gabry, Chuhua Zhang, Zhiqiang Wang
BACKGROUND: It is documented that in tumor cell lines, the hTERT gene exhibits prominent methylation at a CpG island rich region about -600 bp upstream of the transcription start site, but mixed or allelically absent around ±150 bp region. Given the potential clinical implications of the findings in breast cancer diagnostics, we set out to investigate if such findings are reproducible on primary surgically resected invasive breast carcinomas. METHODS: The cohort consisted of 50 cases of freshly sampled and formalin-fixed paraffin embedded (FFPE) invasive breast cancers and normal tissue...
November 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27614471/clonal-lymphoproliferations-in-a-patient-with-common-variable-immunodeficiency
#19
Sarah A Williams, Laura E Moench, Fatima Khan, Gregory Vercellotti, Michael A Linden
Common variable immunodeficiency (CVID) comprises a heterogeneous group of disorders of humoral immunity, characterized by marked deficiencies in serum immunoglobulins. Immune dysregulation causes susceptibility to recurrent bacterial infections, as well as autoimmune and lymphoproliferative disorders. Although the lymphoid cells comprising the atypical proliferations are often clonally related, their malignant potential and clinical significance differ from similar lesions in individuals with immunocompetence...
November 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27593170/the-effectiveness-of-temperature-versus-aprotinin-in-maintaining-the-preanalytical-stability-of-adrenocorticotrophin
#20
Hakan Turkon, Burak Toprak, Hulya Yalcin, Ayfer Colak, Nurinnisa Ozturk
OBJECTIVE: To investigate the effect of temperature and protease inhibitor on adrenocorticotrophin (ACTH) stability. METHODS: We collected blood specimens from 10 donors into K3 EDTA (ethylenediaminetetraacetic acid) and K3 EDTA + aprotinin tubes. Plasma specimens were held at room temperature and at 2 °C to 8[Formula: see text] for 0, 2, 4, 8, 24, and 72 hours. ACTH concentrations in 4 storage conditions were compared with repeated-measures analysis of variance (ANOVA) at each time point...
November 2016: Laboratory Medicine
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