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Laboratory Medicine

Burak Bahar, Stefan E Pambuccian, Güliz A Barkan, Yasemin Akdas
Background: The misuse of statistical methods in diagnostic accuracy studies has been criticized in many publications. Objective: To assess the use and misuse of statistical methods in medical journal articles. Methods: We reviewed 141 original articles from 6 cytopathology journals published in 2014. Results: In total, 16 articles used no descriptive statistics and 66 articles used no inferential statistics. Also, 82 articles did not report using any tests for diagnostic accuracy...
July 17, 2018: Laboratory Medicine
Egarit Noulsri, Sakkarin Ardsiri, Surada Lerdwana, Kovit Pattanapanyasat
Objective: To determine the number and intensity of phosphatidylserine (PS) expression of the red blood cells (RBCs), fragmented RBCs, and RBC-derived microparticles (RMPs) in patients with β-thalassemia/hemoglobin (Hb)E. Methods: We used flow cytometry to determine the number and levels of PS expression. Results: The number of PS-exposing RBCs was statistically significantly higher (P <.001) than that of PS-exposing fragmented RBCs or RMPs...
July 17, 2018: Laboratory Medicine
Chethan K Belludi, Edward T Qian, James J Tolle, Ryan M Brown, Mary Ann Thompson, Ridas Juskevicius
Anaplastic large cell lymphoma (ALCL) is a lymphoma of T-cell origin, characterized by the presence of large lymphoid cells with abundant cytoplasm and pleomorphic, often horseshoe-shaped nuclei (hallmark cells), as well as strong and uniform expression of cluster of differentiation (CD)30. Two distinct clinicopathologic categories of ALCL include primary cutaneous ALCL and systemic ALCL. Systemic ALCL is further classified into anaplastic lymphoma kinase (ALK)-positive, ALK-negative, and breast implant-associated ALCL...
July 17, 2018: Laboratory Medicine
Ashwin S Akki, Sun M Chung, Bryan J Rudolph, Michelle R Ewart
Background: We describe the differential diagnosis of an obese 12-year-old boy of Mexican origin who presented with a 6-year history of abnormal lipid profile and elevated liver transaminase levels. Methods: The patient underwent routine clinical testing, an abdominal ultrasound and, ultimately, a liver biopsy. Based on the histologic findings, a serum leukocyte lysosomal acid lipase (LAL) assay and DNA sequencing of the lipase A (LIPA) gene were performed. Results: Liver biopsy revealed diffuse microvesicular steatosis with clusters of foamy histiocytes in the lobules and portal areas...
July 4, 2018: Laboratory Medicine
Hoi-Ying Elsie Yu, Harold Lanzoni, Tracy Steffen, Warren Derr, Kim Cannon, Jeanene Contreras, Jordan Erik Olson
Background: To further improve workflow efficiency, our laboratory implemented a total laboratory automation (TLA) system that connected our preanalytic processing system with various testing (hematology, coagulation, and chemistry). Methods: Detailed time and motion studies were performed to create process flow maps before and after TLA. The before maps identified opportunities for workflow improvements. We used postimplementation studies to quantify efficiency gains...
June 29, 2018: Laboratory Medicine
Tingting Li, Haijian Zhao, Cuanbao Zhang, Wei Wang, Falin He, Kun Zhong, Shuai Yuan, Zhiguo Wang
Objective: To understand the reasons for proficiency testing (PT) failures, toward the goal of continuous improvement of laboratory services. Methods: Laboratories participating in PT of routine chemistry testing provided by the National Center for Clinical Laboratories (NCCL) of China were given 3 schemes for handling PT failures in 2016. If they had unsatisfactory PT performance, they were notified and requested to investigate failure errors and submit data regarding those errors...
June 28, 2018: Laboratory Medicine
Kimberly W Sanford, Jennifer Anderson, Susan Roseff, Richard A McPherson
Objective: To describe the indicidence and severity of iron deficiency anemia (IDA) in patients who have received extracorporeal photopheresis (ECP) treatment of cutaneous T-cell lymphoma (CTCL). Methods: We performed a retrospective study during a 9-year period of patients with CTCL who were treated with ECP. ECP was performed with UVAR XTS and CELLEX (Therakos Inc). IDA was defined by a drop in hemoglobin (Hb), mean cell volume (MCV), and increased red blood cell distribution width (RDW)...
June 28, 2018: Laboratory Medicine
Alejandro Ruiz-Argüelles, Jose M Gastélum-Cano, Mariana A Méndez-Huerta, Alma B Rodríguez-Gallegos, Guillermo J Ruiz-Argüelles
Background: Glomerular filtration rate (GFR) is partially impaired in patients with multiple sclerosis (MS). When given chemotherapy before receiving hematopoietic stem-cell transplantation, GFR might be further deteriorated. Objective: To measure the effect of cyclophosphamide on GFR in patients with MS who undergo chemotherapy. Methods: We estimated GFR based on creatinine and cystatin C plasma concentrations in patients undergoing autologous hematopoietic stem-cell transplantation to treat their MS...
June 15, 2018: Laboratory Medicine
Chaicharoen Tantanate, Cherdsak Klinbua
Objectives: To investigate the association between high-fluorescence lymphocyte cell (HFLC) and atypical lymphocyte (AL) counts, and to determine the clinical significance of HFLC. Methods: We compared automated HFLC and microscopic AL counts and analyzed the findings. Patient clinical data for each specimen were reviewed. Results: A total of 320 blood specimens were included. The correlation between HFLC and microscopic AL counts was 0.865 and 0...
June 15, 2018: Laboratory Medicine
Mrigender S Virk, Nathan P Dean, Edward C C Wong
Current chemistry analyzers measure ion concentration using ion- selective electrodes; however, may differ in the specific technology at the bedside versus the central laboratory. Instruments utilized for point-of-care testing (POCT) at the bedside use direct ion-selective electrodes, whereas central-laboratory analyzers use indirect ion-selective electrodes. Under most circumstances, these instruments will deliver the same result; however, various substances can cause interferences in one or the other. An 18-year-old Hispanic woman with a history of immune thrombocytopenic purpura (ITP) presented at Children's National Medical Center (CNMC) with a severe headache and required intravenous immunoglobulin (IVIG) therapy...
June 12, 2018: Laboratory Medicine
Rekha Gautam, Joo-Yeun Oh, Marisa B Marques, Richard A Dluhy, Rakesh P Patel
Background: The therapeutic efficacy and safety of stored red blood cells (RBCs) relies on minimal in-bag hemolysis. The accuracy of current methods of measuring hemolysis can suffer as a result of specimen collection and processing artefacts. Objective: To test whether Raman spectroscopy could be used to assess hemolysis. Methods: RBCs were stored for as long as 42 days. Raman spectra of RBCs were measured before and after washing, and hemolysis was measured in supernatant by visible spectroscopy...
June 11, 2018: Laboratory Medicine
Soheila Rahmanzadeh-Shahi, Ali Golshiri-Isfahani, Mahmood Sheikh Fathollahi, Mohammadtaghi Rezayati, Reza Bahramabadi, Mohammadreza Afrooz, Gholamreza Asadikaram, Derek Kennedy, Mohammad Kazemi Arababadi
Objective: To evaluate the serum levels of interleukin (IL)-6, IL-8, tumor necrosis factor (TNF)-α, tumor growth factor (TGF)-β, endothelin, and immunoglobulin (Ig)E in patients with idiopathic epistaxis, compared with healthy control individuals. Methods: Serum levels of IL-6, IL-8, TNF-α, TGF-β, endothelin, and IgE were evaluated in 110 patients with idiopathic epistaxis and 100 healthy controls using the enzyme-linked immunosorbent assay (ELISA) technique...
June 9, 2018: Laboratory Medicine
Eiman Hussein
Background: An efficient apheresis device performs therapeutic plasma exchange (TPE) sessions in short times, while avoiding significant potential cell losses in the removed plasma. Methods: To evaluate TPE performed via Optia (hereinafter, Optia) and COBE Spectra (hereinafter, COBE) apheresis devices, a paired randomized comparison study was conducted. We evaluated the device performance and its impact on cell loss in the removed plasma, as well as blood counts of patients...
May 26, 2018: Laboratory Medicine
Vanessa Garcia Moreira, Nana Beridze Vaktangova, Maria Dolores Martinez Gago, Belen Laborda Gonzalez, Sara Garcia Alonso, Eloy Fernandez Rodriguez
Background: Usually serum albumin is measured with dye-binding assay as bromocresol green (BCG) and bromocresol purple (BCP) methods. The aim of this paper was to examine the differences in albumin measurements between the Advia2400 BCG method (AlbBCG), Dimension RxL BCP (AlbBCP) and capillary zone electrophoresis (CZE). Methods: Albumin concentrations from 165 serum samples were analysed using AlbBCG, AlbBCP and CZE. CZE was employed to estimate different serum protein fractions...
May 22, 2018: Laboratory Medicine
Nessrin Ghazi Alabdallat, Abdul Aziz A Bin Dukhyil
Background: Bloodborne hepatitis B virus (HBV) transmission from asymptomatic donors with acute HBV infections who have undetectable surface antigen of HBV (HBsAg), or from donors with chronic infections in whom serological markers were not detected, could cause residual infections leading to relevant transfusion-transmitted infections (RTTIs). HBV nucleic acid testing (NAT) can detect HBV DNA in the HBsAg-negative and total hepatitis B core antibody (anti-HBc)-negative window period of infection and in chronic cases...
May 15, 2018: Laboratory Medicine
Richard R Gammon, Tania Kozel, Patrick Morel, Christopher Kendrick
Background: Zinc protoporphyrin (ZPP) testing has been studied previously as a substitute for ferritin (fer) testing of blood donors in determining iron stores. Objective: To determine whether ZPP testing could be performed using specimens collected via the capillary (cap) finger-stick (FS) method, similar to predonation hemoglobin (HB) testing. Also, we evaluated the correlation among FS HB, venous (ven) ZZP, FS ZPP, and ven fer levels as predictors of iron depletion...
May 11, 2018: Laboratory Medicine
Zijie Tang, Li Li, Lei Shen, Xianjuan Shen, Shaoqing Ju, Hui Cong
Breast cancer (BC) is one of the most common types of malignant neoplasm in women; the incidence of BC increases yearly. In a previous study, a novel and sensitive method for quantitying cell-free DNA (CFD) in human blood was established and tested for its ability to predict which patients harbored tumors. Our objective in this study was to investigate the clinical value of serum concentration and the integrity of circulating free DNA (CFD) as a biomarker for auxiliary diagnosis of BC. The concentration of CFD was quantitated by branched DNA (bDNA)-based Alu assay...
April 26, 2018: Laboratory Medicine
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No abstract text is available yet for this article.
April 25, 2018: Laboratory Medicine
Maria A Friedlander, Lynnette S Pineault, Amber Donnelly, Sandra Giroux, Kalyani Naik
Introduction: Sustaining a well-trained, competitive, and marketable cytotechnology workforce is a major goal of the American Society of Cytopathology/American Society for Clinical Pathology Workgroup Focusing on Emerging Roles in Cytopathology (ASC/ASCP Workgroup). This article describes the data collection initiatives performed by the Workgroup to confirm cytotechnologist (CT) perceived educational needs to keep pace with emerging practice changes in the workplace. In response to data collected, the Workgroup created Advanced Cytopathology Education (ACE)da unique, innovative educational resource designed to fulfill this need...
July 5, 2018: Laboratory Medicine
Janie Roberson, Asma M Ali, Jennifer Clark, Isam Eltoum, David Ritter, Ryan Soles
Background: The American Society for Clinical Pathology (ASCP) Board of Certification (BOC) surveys US certified cytotechnologists (CTs) at approximately 5-year intervals to gain information about current practice patterns. Although the purpose of this survey is to inform valid content development for the BOC CT examination, comparative longitudinal analysis of the survey data provides information about changes in cytotechnology practice. Materials and Methods: BOC Practice Analysis Survey data for 2009 and 2015 were examined, comparing survey demographics and performance of laboratory tasks...
July 5, 2018: Laboratory Medicine
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