journal
MENU ▼
Read by QxMD icon Read
search

Laboratory Medicine

journal
https://www.readbyqxmd.com/read/29790973/overestimation-of-albumin-measured-by-bromocresol-green-vs-bromocresol-purple-method-influence-of-acute-phase-globulins
#1
Vanessa Garcia Moreira, Nana Beridze Vaktangova, Maria Dolores Martinez Gago, Belen Laborda Gonzalez, Sara Garcia Alonso, Eloy Fernandez Rodriguez
Background: Usually serum albumin is measured with dye-binding assay as bromocresol green (BCG) and bromocresol purple (BCP) methods. The aim of this paper was to examine the differences in albumin measurements between the Advia2400 BCG method (AlbBCG), Dimension RxL BCP (AlbBCP) and capillary zone electrophoresis (CZE). Methods: Albumin concentrations from 165 serum samples were analysed using AlbBCG, AlbBCP and CZE. CZE was employed to estimate different serum protein fractions...
May 22, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29767761/significance-of-hbv-nat-among-hbs-antigen-negative-blood-donors-in-saudi-arabia
#2
Nessrin Ghazi Alabdallat, Abdul Aziz A Bin Dukhyil
Background: Bloodborne hepatitis B virus (HBV) transmission from asymptomatic donors with acute HBV infections who have undetectable surface antigen of HBV (HBsAg), or from donors with chronic infections in whom serological markers were not detected, could cause residual infections leading to relevant transfusion-transmitted infections (RTTIs). HBV nucleic acid testing (NAT) can detect HBV DNA in the HBsAg-negative and total hepatitis B core antibody (anti-HBc)-negative window period of infection and in chronic cases...
May 15, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29757413/evaluation-of-iron-stores-by-zinc-protoporphyrin-analysis-in-blood-donors
#3
Richard R Gammon, Tania Kozel, Patrick Morel, Christopher Kendrick
Background: Zinc protoporphyrin (ZPP) testing has been studied previously as a substitute for ferritin (fer) testing of blood donors in determining iron stores. Objective: To determine whether ZPP testing could be performed using specimens collected via the capillary (cap) finger-stick (FS) method, similar to predonation hemoglobin (HB) testing. Also, we evaluated the correlation among FS HB, venous (ven) ZZP, FS ZPP, and ven fer levels as predictors of iron depletion...
May 11, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29701836/diagnostic-value-of-serum-concentration-and-integrity-of-circulating-cell-free-dna-in-breast-cancer-a-comparative-study-with-cea-and-ca15-3
#4
Zijie Tang, Li Li, Lei Shen, Xianjuan Shen, Shaoqing Ju, Hui Cong
Breast cancer (BC) is one of the most common types of malignant neoplasm in women; the incidence of BC increases yearly. In a previous study, a novel and sensitive method for quantitying cell-free DNA (CFD) in human blood was established and tested for its ability to predict which patients harbored tumors. Our objective in this study was to investigate the clinical value of serum concentration and the integrity of circulating free DNA (CFD) as a biomarker for auxiliary diagnosis of BC. The concentration of CFD was quantitated by branched DNA (bDNA)-based Alu assay...
April 26, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29701829/erratum
#5
(no author information available yet)
No abstract text is available yet for this article.
April 25, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29688441/interference-by-rheumatoid-factor-in-immunoglobulin-m-class-herpes-simplex-virus-types-1-2-immunoassays
#6
Jingjing Pan, Gaili Meng, Bin Huang, Liangzi Lan, Liang Ming
Herpes simplex virus (HSV) types 1 + 2 immunoglobulin (Ig)M antibodies assays are important tools for diagnosing pregnant women and their newborns. However, it is unclear whether high levels of rheumatoid factor (RF) interfere with serological methods used to test for HSV types 1 + 2 type-specific IgM antibodies. We report a case of a false-positive result for HSV 1 + 2 IgM antibodies due to gross overestimation of RF by enzyme-linked immunosorbent assay (ELISA). This result occurred in a 25-year-old Han Chinese woman who was 28 weeks pregnant with a fetus (sex of fetus unknown)...
April 24, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29688520/comparison-of-2-anti-pla2r-immunoassays-for-the-diagnosis-of-primary-membranous-nephropathy
#7
Weihao Li, Yaping Guo, Zhiping Zhang, Feifei Zhang, Xiaomei Liu, Xin Ji, Lixia Liu, Hong Wang
Background: Anti-phospholipase A2 receptor (anti-PLA2R) is a promising biomarker for diagnosis, activity evaluation, therapy monitoring, and prognostic estimation of primary membranous neuropathy (pMN). Difference in methodology may be one cause of the discrepancy in anti-PLA2R-positive percentages in reported studies. In this study, we evaluated the determination consistency of anti-PLA2R using indirect immunofluorescence assay (IIF) and enzyme-linked immunosorbent assay (ELISA). Methods: A total of 113 patients with pMN, 34 with secondary membranous neuropathy (sMN), and 53 healthy control individuals were enrolled...
April 23, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29659973/the-diagnostic-challenge-of-acquired-thrombotic-thrombocytopenic-purpura-in-children-case-report-and-review-of-the-literature
#8
Elise Schapkaitz, Tanya Marié Schickerling
Acquired thrombotic thrombocytopenic purpura (TTP) is a rare disease in children. Despite advances in diagnosis and treatment, acquired TTP remains a challenging disease due to the lack of a simple diagnostic test and the variable response to plasma exchange. Herein, we describe a case of a 5-year-old of black ethnicity boy who presented with a sudden onset of fatigue and body aches. Laboratory investigations revealed a thrombotic microangiopathic manifestation. TTP was diagnosed, and plasma exchange and corticosteroids were initiated, with an excellent response...
April 12, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29608741/critically-elevated-potassium-in-a-55-year-old-female-with-chronic-lymphocytic-leukemia
#9
Jing Cao, Amy B Karger
Hyperkalemia in specimens from patients with chronic lymphocytic leukemia (CLL) may be due to tumor lysis syndrome (TLS) or specimen processing. This report describes a 55-year-old Caucasian woman with CLL who presented to an outside hospital with hyperkalemia and was transferred to a second hospital. Initial evaluation on the core laboratory chemistry analyzer (the VITROS 5600) and the ABL90 FLEX blood gas analyzer showed markedly elevated levels of potassium (K+). TLS was subsequently diagnosed, and dialysis was initiated...
March 28, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29608696/serum-cortisol-levels-via-radioimmunoassay-vs-liquid-chromatography-mass-spectrophotometry-in-healthy-control-subjects-and-patients-with-adrenal-incidentalomas
#10
Martha K P Huayllas, Brian C Netzel, Ravinder J Singh, Claudio E Kater
Background: Adrenal incidentalomas (AIs) are present in 4% of adults. As many as 30% may secrete cortisol autonomously in the absence of specific signs of overt hypercortisolism, in a phenomenon called subclinical hypercortisolism (SH). Diagnosis of SH is established by serum cortisol resistance to dexamethasone suppression. Methods: We compared serum cortisol concentrations, as determined by radioimmunoassay (RIA) and liquid chromatography/tandem mass spectronomy (LC/MS-MS), in 73 patients with AI group (52 with unilateral AI) and 34 control subjects in 3 scenarios: basal; after 1-mg dexamethasone suppression; and after 0...
March 28, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29562243/diagnostic-value-of-serum-smp30-and-anti-smp30-antibody-in-hepatocellular-carcinoma
#11
Shun-Xin Zheng, Bang-De Xiang, Jia-Min Long, Cong Qu, Zhi-Jing Mo, Kun Li, Yuan Zhuang, Zhi-Lue Lv, Su-Fang Zhou
Objective: To evaluate the clinical value of senescence marker protein 30 (SMP30) and anti-SMP30 antibody in serum. Methods: We used enzyme-linked immunosorbent assay (ELISA) to analytically validate serum levels of SMP30 and anti-SMP30 antibody in 143 patients with hepatocellular carcinoma (HCC), compared with those levels in serum from 137 patients with chronic hepatitis (CH), 51 with liver cirrhosis (LC), and 165 healthy control individuals. Results: The positivity rate of SMP30 in the HCC group (8...
March 17, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29566170/comparison-between-a-manual-squamous-cell-carcinoma-antigen-assay-and-an-automated-assay-in-a-clinical-setting
#12
Saejin Lee, Juwon Kim, Young Uh, Hyon-Suk Kim, Jong-Han Lee
Objective: To compare a manual squamous cell carcinoma antigen (SCCA) assay and an automated SCCA assay in a clinical setting. Methods: We included, in this study, a total of 158 specimens that had been tested using the manual SCCA assay. The CanAg SCC EIA assay and the Elecsys SCC were compared for their clinical settings. Results: Within-run and between-day coefficients of variation (CVs, %) were lower than 3%. Comparison of the manual and automated SCCA assays yielded good correlation...
March 16, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29546347/corrected-lymphocyte-percentages-reduce-the-differences-in-absolute-cd4-t-lymphocyte-counts-between-dual-platform-and-single-platform-flow-cytometric-approaches
#13
Egarit Noulsri, Dinar Abudaya, Surada Lerdwana, Kovit Pattanapanyasat
Objective: To determine whether a corrected lymphocyte percentage could reduce bias in the absolute cluster of differentiation (CD)4+ T lymphocyte counts obtained via dual-platform (DP) vs standard single-platform (SP) flow cytometry. Methods: The correction factor (CF) for the lymphocyte percentages was calculated at 6 laboratories. The absolute CD4+ T lymphocyte counts in 300 blood specimens infected with human immunodeficiency virus (HIV) were determined using the DP and SP methods...
March 13, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29528435/bilayered-medium-for-rapid-isolation-of-mycobacterium-tuberculosis
#14
HebatAllah Abd El Monaem Bassiouny, Somaya Mohamed Hussein, Laila Ahmed El-Attar, Amira Ezzat Khamis Amine
Background: A bilayered medium (BLM) seemed to indicate a rapid recovery time from Mycobacterium tuberculosis (M. tuberculosis) for the patients in question, namely, 48 hours. Method: Sputum specimens collected from 112 patients with clinically suspected pulmonary tuberculosis were subjected to Ziehl-Neelsen staining and inoculation on Löwenstein-Jensen medium (LJM) and BLM. Results: BLM grew all the 36 (100%) smear positive samples, while LJM grew only 20 (55...
March 8, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29528429/comparison-of-enzymatic-assay-for-hba1c-measurement-abbott-architect-with-capillary-electrophoresis-sebia-minicap-flex-piercing-analyser
#15
Andrea Tesija Kuna, Kristina Dukic, Nora Nikolac Gabaj, Marijana Miler, Ines Vukasovic, Sanja Langer, Ana-Maria Simundic, Nada Vrkic
Objective: To compare the analytical performances of the enzymatic method (EM) and capillary electrophoresis (CE) for hemoglobin A1c (HbA1c) measurement. Methods: Imprecision, carryover, stability, linearity, method comparison, and interferences were evaluated for HbA1c via EM (Abbott Laboratories, Inc) and CE (Sebia). Results: Both methods have shown overall within-laboratory imprecision of less than 3% for International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) units (<2% National Glycohemoglobin Standardization Program [NGSP] units)...
March 8, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29509926/erratum
#16
(no author information available yet)
No abstract text is available yet for this article.
March 2, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29579275/erratum
#17
(no author information available yet)
No abstract text is available yet for this article.
March 21, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29579274/erratum
#18
(no author information available yet)
No abstract text is available yet for this article.
March 21, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29538693/what-s-a-lab-to-do-during-and-after-a-hurricane
#19
Fred Rodriguez, Rajeevan Selvaratnam, Peggy Mann, Rina Kalariya, John R Petersen
Although laboratories may be able to rely on a comprehensive Hurricane Plan during a hurricane, alarming and unanticipated events frequently occur. To minimize disruption of lab operations, it is important to try to mitigate the impact of these unexpected events as quickly as possible, in the quest to minimize negative outcomes. In this article, we discuss approaches to dealing with unanticipated events during and after hurricanes, based on our personal experiences.
March 21, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29481622/hurricanes-are-you-prepared
#20
Fred H Rodriguez, John Petersen, Rajeevan Selvaratnam, Peggy Mann, Jonathan B Hoyne
Severe weather events such as hurricanes have the potential to cause significant disruption of laboratory operations. Comprehensive planning is essential to mitigate the impact of such events. The essential elements of a Hurricane Plan, based on our personal experiences, are detailed in this article.
March 21, 2018: Laboratory Medicine
journal
journal
22535
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"