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Folia Biologica (Praha)

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https://www.readbyqxmd.com/read/27978417/age-related-aged-vs-adult-comparison-of-the-effect-of-two-mild-stressors-on-the-nerve-growth-factor-ngf-in-the-rat-hypothalamic-supraoptic-nucleus-son-immunohistochemical-study
#1
E Badowska-Szalewska, B Ludkiewicz, R Krawczyk, J Moryś
The ontogenetic period of life and stress can have different effects on the nerve growth factor (NGF) in the hypothalamus. The aim of our study was to investigate the influence of two mild stressors, acute and chronic exposure to forced swim (FS) or high-light open field (HL-OF), on neurons containing NGF. Immunofluorescence staining was used to reveal the density of NGF-immunoreactive (ir) cells in the hypothalamic supraoptic nucleus (SON) in adult (postnatal day 90; P90) and aged (P720) rats. The P90 and P720 rats that were subjected to acute and chronic FS showed no differences in the density of NGF-ir neurons in the SON compared with nonstressed rats...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27978416/apoptosis-in-endomyocardial-biopsies-from-patients-with-dilated-cardiomyopathy
#2
S Glumac, S Pejić, S Kostadinovic, Z Stojšić, J Vasiljevic
Apoptosis is an active energy-consuming mechanism of cell death, which may contribute to heart failure in patients with dilated cardiomyopathy. Dilated cardiomyopathy is a common clinical outcome of many prolonged cardiac insults, and therefore is considered as the most prevalent form of cardiomyopathy. Loss of heart mass is highly correlated with the heart failure and mortality, thus the purpose of this study was to define the apoptotic index in patients with dilated cardiomyopathy. Apoptosis was detected by the TUNEL method in 30 patients...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27978415/detection-of-mycoplasma-contamination-directly-from-culture-supernatant-using-polymerase-chain-reaction
#3
R V Pisal, H Hrebíková, J Chvátalová, D Kunke, S Filip, J Mokrý
Ensuring mycoplasma-free cell culture is of prime importance as they severely affect cellular characteristics leading to experimental artefacts and spurious results. Various methods persist for mycoplasma detection; out of the whole array of methods polymerase chain reaction (PCR) is the most favoured one because it is highly sensitive, specific and quick. The PCR-based detection procedure involves three steps: cell culture supernatant collection, DNA isolation, and PCR. We have modified this procedure so that cell culture supernatant can directly be used for PCR without the need for DNA extraction...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27978414/idh1-2-mutation-and-mgmt-promoter-methylation-the-relevant-survival-predictors-in-czech-patients-with-brain-gliomas
#4
F Kramář, M Minárik, L Benešová, T Halková, D Netuka, O Bradáč, V Beneš
Gliomas are a heterogeneous group of tumours varying in prognosis, treatment approach, and overall survival. Recently, novel markers have been identified which are linked to patient prognosis and therapeutic response. Especially the mutation of the enzyme isocitrate dehydrogenase 1 or 2 (IDH1/2) gene and the O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation status seem to be the most important predictors of survival. From 2012 to 2015, 94 Czech patients with primary brain tumours were enrolled into the study...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27978413/can-leukocyte-telomere-length-predict-survival-time-in-heart-transplant-recipients-over-a-minimal-follow-up-of-20-years
#5
D Dlouhá, V Vančura, J Vymětalová, J A Hubáček, V Lánská, I Málek
In humans, leukocyte telomere length (LTL) reduces with age and is reported to be inversely associated with ageing-related diseases. We measured LTL in leukocyte DNA using a quantitative PCR-based method from 127 blood samples of heart recipients (107 males, 20 females, age 44.1 ± 10.5), followed for up to 30 years. Patients with coronary artery disease survived for a shorter time and also had shorter LTL (both P < 0.05 after adjustment for age and sex) than subjects with dilated cardiomyopathy. Patients with non-cardiac causes of death had shorter LTL than patients with cardiac causes (P < 0...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27978412/the-potential-role-of-melatonin-on-memory-function-lessons-from-rodent-studies
#6
R Zakaria, A H Ahmad, Z Othman
Pineal melatonin biosynthesis is regulated by the circadian clock located in the suprachiasmatic nucleus of the hypothalamus. Melatonin has been found to modulate the learning and memory process in human as well as in animals. Endogenous melatonin modulates the process of newly acquired information into long-term memory, while melatonin treatment has been found to reduce memory deficits in elderly people and in various animal models. However, the mechanisms mediating the enhancing effect of melatonin on memory remain elusive...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27643583/conditional-knockout-of-the-microrna-17-92-cluster-in-type-i-collagen-expressing-cells-decreases-alveolar-bone-size-and-incisor-tooth-mechanical-properties
#7
M Ibrahim, S Mohan, M J Xing, C Kesavan
To test the role of the miR17-92 (miR) cluster in dental bones, we evaluated the incisor tooth phenotype by micro-CT in 5- and 12-week-old conditional knockout (CKO) mice deficient in the miR17-92 cluster in type-I collagen-expressing cells and bone strength by finite element analysis. The incisor teeth of CKO mice showed a 23-30 % reduction in tissue volume and bone volume. Accordingly, the stiffness and failure load of incisor teeth assessed by finite element analysis showed an 18-40 % decrease in CKO compared to wild-type mice...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27643582/comparison-of-the-radiosensitizing-effect-of-atr-atm-and-dna-pk-kinase-inhibitors-on-cervical-carcinoma-cells
#8
J Vávrová, L Zárybnická, P Jošt, A Tichý, M Řezáčová, Z Šinkorová, J Pejchal
Here, we compared the effects of inhibitors of three phosphatidylinositol-3-kinase-related kinases, ATM, ATR a DNA-PK, on radiosensitization of cervical carcinoma cells. We demonstrated that DNA-PK inhibitor NU7441 enhanced phosphorylation of Chk1 and Chk2 kinases 2 h after irradiation of HeLa cells at a dose of 8 Gy in contrast to ATM kinase inhibitor KU55933, which completely blocked the Chk2 kinase phosphorylation on threonine 68, and ATR kinase inhibitor VE-821, which blocked the Chk1 kinase phosphorylation on serine 345...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27643581/mast-cells-might-have-a-protective-role-against-the-development-of-calcification-and-hyalinisation-in-severe-aortic-valve-stenosis
#9
A Milutinovic, D Petrovič, M Zorc, O Vraspir Porenta, M Arko, A Pleskovič, A Alibegovic, R Zorc-Pleskovic
Aortic valve stenosis is characterized by inflammation and extracellular matrix remodelling. The aim of this study was to analyse the impact of mast cells on the occurrence of histopathological changes of aortic valves in patients with severe grade, non-rheumatic degenerative aortic valve stenosis. Valve specimens were obtained from 38 patients undergoing valve replacement. The role of mast cells was analysed by dividing the specimens into two groups, characterized by the presence (group A, N = 13) or absence of mast cells (group B, N = 25)...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27643580/changes-in-liver-ganglioside-metabolism-in-obstructive-cholestasis-the-role-of-oxidative-stress
#10
V Šmíd, T Petr, K Váňová, J Jašprová, J Šuk, L Vítek, F Šmíd, L Muchová
Bile acids have been implicated in cholestatic liver damage, primarily due to their detergent effect on membranes and induction of oxidative stress. Gangliosides can counteract these harmful effects by increasing the rigidity of the cytoplasmic membrane. Induction of haem oxygenase (HMOX) has been shown to protect the liver from increased oxidative stress. The aim of this study was to determine the changes in the synthesis and distribution of liver gangliosides following bile duct ligation (BDL), and to assess the effects of HMOX both on cholestatic liver injury and ganglioside metabolism...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27643579/neurological-deficits-of-an-rps19-arg67del-model-of-diamond-blackfan-anaemia
#11
A Kubik-Zahorodna, B Schuster, I Kanchev, R Sedláček
Diamond-Blackfan anaemia is a rare disease caused by insufficient expression of ribosomal proteins and is characterized by erythroid hypoplasia often accompanied by growth retardation, congenital craniofacial and limb abnormalities. In addition, Diamond-Blackfan anaemia patients also exhibit a number of behavioural abnormalities. In this study we describe the behavioural effects observed in a new mouse mutant carrying a targeted single amino acid deletion in the ribosomal protein RPS19. This mutant, created by the deletion of arginine 67 in RPS19, exhibits craniofacial, skeletal, and brain abnormalities, accompanied by various neurobehavioural malfunctions...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27516192/down-regulation-of-aqp4-inhibits-proliferation-migration-and-invasion-of-human-breast-cancer-cells
#12
Y-B Li, S-R Sun, X-H Han
Aquaporins (AQPs), proteinaceous water channels, have been proposed as mediators of tumour development and progression. However, the role of aquaporin 4 (AQP4), a member of the AQP family, in breast cancer has not been distinctly evaluated. The aim of the present study was to examine the effect of AQP4 down-regulation on proliferation, migration and invasion in human breast cancer. To determine this effect, siRNA interference was used to knock down its expression in T47D and MCF-7 cell lines. Down-regulation of AQP4 resulted in increased expression of E-cadherin along with an inhibitory effect on the proliferation, migration and invasion in breast cancer cells...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27516191/the-quality-control-of-mesenchymal-stromal-cells-by-in-vitro-testing-of-their-immunomodulatory-effect-on-allogeneic-lymphocytes
#13
D Lysák, L Koutová, M Holubová, T Vlas, M Miklíková, P Jindra
Mesenchymal stromal cells (MSC) represent a promising treatment of graft-versus-host disease (GVHD) in patients after allogeneic haematopoietic stem cell transplantation. We performed co-cultivation experiments with non-specifically stimulated lymphocytes to characterize the immunosuppressive activity of MSC. MSC influenced expression of some activation antigens. CD25 expression was lower with MSC and reached 55.2 % vs. 84.9 % (CD4+, P = 0.0006) and 38.8 % vs. 86.6 % (CD8+, P = 0.0003) on day +4. Conversely, CD69 antigen expression remained higher with MSC (73...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27516190/importance-of-tumour-suppressor-gene-methylation-in-sinonasal-carcinomas
#14
M Chmelařová, I Sirák, M Mžik, K Sieglová, H Vošmiková, P Dundr, K Němejcová, J Michálek, M Vošmik, V Palička, J Laco
Epigenetic changes are considered to be a frequent event during tumour development. Hypermethylation of promoter CpG islands represents an alternative mechanism for inactivation of tumour suppressor genes, DNA repair genes, cell cycle regulators and transcription factors. The aim of this study was to investigate promoter methylation of specific genes in samples of sinonasal carcinoma by comparison with normal sinonasal tissue. To search for epigenetic events we used methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) to compare the methylation status of 64 tissue samples of sinonasal carcinomas with 19 control samples...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27516189/generation-of-gfp-native-protein-for-detection-of-its-intracellular-uptake-by-cell-penetrating-peptides
#15
S Kadkhodayan, S M Sadat, S Irani, F Fotouhi, A Bolhassani
Different types of lipid- and polymer-based vectors have been developed to deliver proteins into cells, but these methods showed relatively poor efficiency. Recently, a group of short, highly basic peptides known as cell-penetrating peptides (CPPs) were used to carry polypeptides and proteins into cells. In this study, expression and purification of GFP protein was performed using the prokaryotic pET expression system. We used two amphipathic CPPs (Pep-1 and CADY-2) as a novel delivery system to transfer the GFP protein into cells...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27516188/iron-overload-causes-alterations-of-e-cadherin-in-the-liver
#16
Y Fujikura, J Krijt, C Povýšil, Z Mělková, P Přikryl, M Vokurka, E Nečas
Iron overload causes tissue damage in the liver, but its initial effects at the molecular and cellular level are not well understood. Epithelial cadherin (E-cad) is a major adhesion protein in adherens junctions and is associated with several signal transduction pathways. Dysfunction of E-cad causes instability of adherens junctions, which leads to cell invasion, cell migration, and carcinogenesis. We found in liver samples from iron-overloaded mice that the apparent molecular mass of E-cad was reduced from 125 to 115 kDa in sodium dodecyl sulphate polyacrylamide gel electrophoresis under reducing conditions and immunoblotting, and that the cellular expression of E-cad was decreased in immunohistochemistry...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27187041/intracellular-delivery-of-synthetic-dsrna-to-leukemic-cells-induces-apoptotic-and-necrotic-cell-death
#17
S M Mahmud, K J Mek, A Idris
The type of tumour cell death dictates the type of adaptive immune response mounted against the tumours. In haematological malignancies such as acute myeloid leukaemia (AML), immune evasion due to the poor immunogenicity of leukemic cells is a major hurdle in generating an effective immune response. Transfection of synthetic dsRNA, poly I:C, into leukemic cells to trigger tumour cell death and enhance immunogenicity of the tumour is a promising immunotherapeutic approach. However, the temporal cell death kinetics of poly I:C-electroporated AML cells has not been thoroughly investigated...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27187040/x-chromosome-inactivation-analysis-in-different-cell-types-and-induced-pluripotent-stem-cells-elucidates-the-disease-mechanism-in-a-rare-case-of-mucopolysaccharidosis-type-ii-in-a-female
#18
M Řeboun, J Rybová, R Dobrovolný, J Včelák, T Veselková, G Štorkánová, D Mušálková, M Hřebíček, J Ledvinová, M Magner, J Zeman, K Pešková, L Dvořáková
Mucopolysaccharidosis type II (MPS II) is an X-linked lysosomal storage disorder resulting from deficiency of iduronate-2-sulphatase activity. The disease manifests almost exclusively in males; only 16 symptomatic heterozygote girls have been reported so far. We describe the results of X-chromosome inactivation analysis in a 5-year-old girl with clinically severe disease and heterozygous mutation p.Arg468Gln in the IDS gene. X inactivation analysed at three X-chromosome loci showed extreme skewing (96/4 to 99/1) in two patient's cell types...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27187039/timp4-modulates-er-%C3%AE-signalling-in-mcf7-breast-cancer-cells
#19
F Pruefer, K Vazquez-Santillan, L Munoz-Galindo, J L Cruz-Colin, V Maldonado, J Melendez-Zajgla
Tissue inhibitor of metalloprotease 4 (TIMP4) contributes to poor prognosis in breast and other tumours. However, the mechanisms of how TIMP4 influences breast cancer cell behaviour are unknown. Our aim was to explore the signalling pathways modulated by TIMP4 in breast cancer cells. Human recombinant TIMP4 was added to MCF7 breast cancer cells and RNASeq was performed. TIMP4 RNASeq results were validated by RT-PCR. Network analyses of TIMP4-exposed cells showed that ER-α, HIF1A and TGF-β signalling were activated, whereas FOXO3 signalling was downregulated...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27187038/novel-cdkl5-mutations-in-czech-patients-with-phenotypes-of-atypical-rett-syndrome-and-early-onset-epileptic-encephalopathy
#20
D Záhoráková, M Langová, K Brožová, J Laštůvková, Z Kalina, L Rennerová, P Martásek
The X-linked CDKL5 gene, which encodes cyclin-dependent kinase-like 5 protein, has been implicated in early-onset encephalopathy and atypical Rett syndrome with early-onset seizures. The CDKL5 protein is a kinase required for neuronal development and morphogenesis, but its precise functions are still largely unexplored. Individuals with CDKL5 mutations present with severe global developmental delay, intractable epilepsy, and Rett-like features. A clear genotype-phenotype correlation has not been established due to an insufficient number of reported cases...
2016: Folia Biologica (Praha)
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