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Folia Biologica (Praha)

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https://www.readbyqxmd.com/read/28189150/evaluation-of-epidermal-neural-crest-stem-cells-in-organotypic-spinal-cord-slice-culture-platform
#1
S Pandamooz, M S Salehi, M Nabiuni, L Dargahi, M Pourghasem
Among various strategies employed for spinal cord injury, stem cell therapy is a potential treatment. So far, a variety of stem cells have been evaluated in animal models and humans with spinal cord injury, and epidermal neural crest stem cells represent one of the attractive types in this area. Although these multipotent stem cells have been assessed in several spinal cord injury models by independent laboratories, extensive work remains to be done to ascertain whether these cells can safely improve the outcome following human spinal cord injury...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/28189149/comparison-of-plasma-osteopontin-levels-between-patients-with-borderline-ovarian-tumours-and-serous-ovarian-carcinoma
#2
COMPARATIVE STUDY
J H Živný, S Leahomschi, P Klener, J Živný, M Haluzík, D Cibula
Osteopontin (OPN) is a novel biomarker of various cancers including ovarian carcinoma. OPN is a promising adjunct to a major biomarker of ovarian cancer, CA125, in diagnosis, differential diagnosis and prognosis. The aim of our study was to measure the plasma level of OPN and CA125 in patients with borderline ovarian tumours (BOTs), serous ovarian carcinoma, and controls to determine its potential role in the differential diagnosis between serous ovarian carcinoma and BOT. The plasma samples of 66 women were analysed using Luminex technology, designed to simultaneously measure multiple specific protein targets...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/28189148/anxiety-and-hyperlocomotion-induced-by-chronic-unpredictable-mild-stress-can-be-moderated-with-melatonin-treatment
#3
N Spasojevic, B Stefanovic, P Jovanovic, S Dronjak
Preclinical studies have shown that melatonin exercised antidepressant-like and anxiolyticlike effects in animal models of anxiety. The aim of the present study was to correlate the changes in behaviour induced by melatonin treatment with the activity of the dopaminergic system in the hippocampus of Wistar rats exposed to chronic, unpredictable, mild stress (CUMS). Male Wistar rats, 11 weeks old, were subjected to chronic stress for 28 successive days. Separate groups of control and stressed rats were intraperitoneally injected daily either with melatonin (10 mg/kg/day, i...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/28189147/optimization-and-validation-of-elisa-for-pre-clinical-trials-of-influenza-vaccine
#4
K Mitic, L Muhandes, R Minic, V Petrusic, I Zivkovic
Testing of every new vaccine involves investigation of its immunogenicity, which is based on monitoring its ability to induce specific antibodies in animals. The fastest and most sensitive method used for this purpose is enzyme-linked immunosorbent assay (ELISA). However, commercial ELISA kits with whole influenza virus antigens are not available on the market, and it is therefore essential to establish an adequate assay for testing influenza virusspecific antibodies. We developed ELISA with whole influenza virus strains for the season 2011/2012 as antigens and validated it by checking its specificity, accuracy, linearity, range, precision, and sensitivity...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/28189146/the-relationship-of-kynurenine-and-neopterin-levels-and-their-association-with-a-selection-of-other-immune-markers-in-chronic-myeloid-leukaemia-patients
#5
V Vonka, M Petráčková, Z Humlová, H Klamová, L Kujovská-Krčmová, M Dušková, B Procházka
Among malignant diseases, chronic myeloid leukaemia (CML) is one of the best suited candidates for immunotherapy. For this purpose it is necessary to broaden the present knowledge on the immunology of this disease. As a part of such a project, the levels of kynurenine (KYN) and neopterin (NPT) were studied in 28 CML patients and in the same number of healthy subjects. At diagnosis, both KYN and NPT levels were found to be elevated in a significant portion of the patients and dependent on their leukocyte count...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/28189145/cystatin-c-is-associated-with-the-extent-and-characteristics-of-coronary-atherosclerosis-in-patients-with-preserved-renal-function
#6
A Král, T Kovárník, Z Vaníčková, H Skalická, J Horák, K Bayerová, Z Chen, A Wahle, L Zhang, K Kopřiva, H Benáková, M Sonka, A Linhart
Cystatin C (CysC), an endogenous inhibitor of cysteine proteases and a sensitive and accurate marker of renal function, is associated with the severity of coronary atherosclerosis assessed by angiography and future cardiovascular events according to previous studies. We aimed to evaluate the association between CysC levels and coronary plaque volume, composition and phenotype assessed by intravascular ultrasound and intravascular ultrasound-derived virtual histology in patients with preserved renal function...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27978417/age-related-aged-vs-adult-comparison-of-the-effect-of-two-mild-stressors-on-the-nerve-growth-factor-ngf-in-the-rat-hypothalamic-supraoptic-nucleus-son-immunohistochemical-study
#7
COMPARATIVE STUDY
E Badowska-Szalewska, B Ludkiewicz, R Krawczyk, J Moryś
The ontogenetic period of life and stress can have different effects on the nerve growth factor (NGF) in the hypothalamus. The aim of our study was to investigate the influence of two mild stressors, acute and chronic exposure to forced swim (FS) or high-light open field (HL-OF), on neurons containing NGF. Immunofluorescence staining was used to reveal the density of NGF-immunoreactive (ir) cells in the hypothalamic supraoptic nucleus (SON) in adult (postnatal day 90; P90) and aged (P720) rats. The P90 and P720 rats that were subjected to acute and chronic FS showed no differences in the density of NGF-ir neurons in the SON compared with nonstressed rats...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27978416/apoptosis-in-endomyocardial-biopsies-from-patients-with-dilated-cardiomyopathy
#8
S Glumac, S Pejić, S Kostadinovic, Z Stojšić, J Vasiljevic
Apoptosis is an active energy-consuming mechanism of cell death, which may contribute to heart failure in patients with dilated cardiomyopathy. Dilated cardiomyopathy is a common clinical outcome of many prolonged cardiac insults, and therefore is considered as the most prevalent form of cardiomyopathy. Loss of heart mass is highly correlated with the heart failure and mortality, thus the purpose of this study was to define the apoptotic index in patients with dilated cardiomyopathy. Apoptosis was detected by the TUNEL method in 30 patients...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27978415/detection-of-mycoplasma-contamination-directly-from-culture-supernatant-using-polymerase-chain-reaction
#9
R V Pisal, H Hrebíková, J Chvátalová, D Kunke, S Filip, J Mokrý
Ensuring mycoplasma-free cell culture is of prime importance as they severely affect cellular characteristics leading to experimental artefacts and spurious results. Various methods persist for mycoplasma detection; out of the whole array of methods polymerase chain reaction (PCR) is the most favoured one because it is highly sensitive, specific and quick. The PCR-based detection procedure involves three steps: cell culture supernatant collection, DNA isolation, and PCR. We have modified this procedure so that cell culture supernatant can directly be used for PCR without the need for DNA extraction...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27978414/idh1-2-mutation-and-mgmt-promoter-methylation-the-relevant-survival-predictors-in-czech-patients-with-brain-gliomas
#10
F Kramář, M Minárik, L Benešová, T Halková, D Netuka, O Bradáč, V Beneš
Gliomas are a heterogeneous group of tumours varying in prognosis, treatment approach, and overall survival. Recently, novel markers have been identified which are linked to patient prognosis and therapeutic response. Especially the mutation of the enzyme isocitrate dehydrogenase 1 or 2 (IDH1/2) gene and the O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation status seem to be the most important predictors of survival. From 2012 to 2015, 94 Czech patients with primary brain tumours were enrolled into the study...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27978413/can-leukocyte-telomere-length-predict-survival-time-in-heart-transplant-recipients-over-a-minimal-follow-up-of-20-years
#11
D Dlouhá, V Vančura, J Vymětalová, J A Hubáček, V Lánská, I Málek
In humans, leukocyte telomere length (LTL) reduces with age and is reported to be inversely associated with ageing-related diseases. We measured LTL in leukocyte DNA using a quantitative PCR-based method from 127 blood samples of heart recipients (107 males, 20 females, age 44.1 ± 10.5), followed for up to 30 years. Patients with coronary artery disease survived for a shorter time and also had shorter LTL (both P < 0.05 after adjustment for age and sex) than subjects with dilated cardiomyopathy. Patients with non-cardiac causes of death had shorter LTL than patients with cardiac causes (P < 0...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27978412/the-potential-role-of-melatonin-on-memory-function-lessons-from-rodent-studies
#12
REVIEW
R Zakaria, A H Ahmad, Z Othman
Pineal melatonin biosynthesis is regulated by the circadian clock located in the suprachiasmatic nucleus of the hypothalamus. Melatonin has been found to modulate the learning and memory process in human as well as in animals. Endogenous melatonin modulates the process of newly acquired information into long-term memory, while melatonin treatment has been found to reduce memory deficits in elderly people and in various animal models. However, the mechanisms mediating the enhancing effect of melatonin on memory remain elusive...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27643583/conditional-knockout-of-the-microrna-17-92-cluster-in-type-i-collagen-expressing-cells-decreases-alveolar-bone-size-and-incisor-tooth-mechanical-properties
#13
M Ibrahim, S Mohan, M J Xing, C Kesavan
To test the role of the miR17-92 (miR) cluster in dental bones, we evaluated the incisor tooth phenotype by micro-CT in 5- and 12-week-old conditional knockout (CKO) mice deficient in the miR17-92 cluster in type-I collagen-expressing cells and bone strength by finite element analysis. The incisor teeth of CKO mice showed a 23-30 % reduction in tissue volume and bone volume. Accordingly, the stiffness and failure load of incisor teeth assessed by finite element analysis showed an 18-40 % decrease in CKO compared to wild-type mice...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27643582/comparison-of-the-radiosensitizing-effect-of-atr-atm-and-dna-pk-kinase-inhibitors-on-cervical-carcinoma-cells
#14
COMPARATIVE STUDY
J Vávrová, L Zárybnická, P Jošt, A Tichý, M Řezáčová, Z Šinkorová, J Pejchal
Here, we compared the effects of inhibitors of three phosphatidylinositol-3-kinase-related kinases, ATM, ATR a DNA-PK, on radiosensitization of cervical carcinoma cells. We demonstrated that DNA-PK inhibitor NU7441 enhanced phosphorylation of Chk1 and Chk2 kinases 2 h after irradiation of HeLa cells at a dose of 8 Gy in contrast to ATM kinase inhibitor KU55933, which completely blocked the Chk2 kinase phosphorylation on threonine 68, and ATR kinase inhibitor VE-821, which blocked the Chk1 kinase phosphorylation on serine 345...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27643581/mast-cells-might-have-a-protective-role-against-the-development-of-calcification-and-hyalinisation-in-severe-aortic-valve-stenosis
#15
A Milutinovic, D Petrovič, M Zorc, O Vraspir Porenta, M Arko, A Pleskovič, A Alibegovic, R Zorc-Pleskovic
Aortic valve stenosis is characterized by inflammation and extracellular matrix remodelling. The aim of this study was to analyse the impact of mast cells on the occurrence of histopathological changes of aortic valves in patients with severe grade, non-rheumatic degenerative aortic valve stenosis. Valve specimens were obtained from 38 patients undergoing valve replacement. The role of mast cells was analysed by dividing the specimens into two groups, characterized by the presence (group A, N = 13) or absence of mast cells (group B, N = 25)...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27643580/changes-in-liver-ganglioside-metabolism-in-obstructive-cholestasis-the-role-of-oxidative-stress
#16
V Šmíd, T Petr, K Váňová, J Jašprová, J Šuk, L Vítek, F Šmíd, L Muchová
Bile acids have been implicated in cholestatic liver damage, primarily due to their detergent effect on membranes and induction of oxidative stress. Gangliosides can counteract these harmful effects by increasing the rigidity of the cytoplasmic membrane. Induction of haem oxygenase (HMOX) has been shown to protect the liver from increased oxidative stress. The aim of this study was to determine the changes in the synthesis and distribution of liver gangliosides following bile duct ligation (BDL), and to assess the effects of HMOX both on cholestatic liver injury and ganglioside metabolism...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27643579/neurological-deficits-of-an-rps19-arg67del-model-of-diamond-blackfan-anaemia
#17
A Kubik-Zahorodna, B Schuster, I Kanchev, R Sedláček
Diamond-Blackfan anaemia is a rare disease caused by insufficient expression of ribosomal proteins and is characterized by erythroid hypoplasia often accompanied by growth retardation, congenital craniofacial and limb abnormalities. In addition, Diamond-Blackfan anaemia patients also exhibit a number of behavioural abnormalities. In this study we describe the behavioural effects observed in a new mouse mutant carrying a targeted single amino acid deletion in the ribosomal protein RPS19. This mutant, created by the deletion of arginine 67 in RPS19, exhibits craniofacial, skeletal, and brain abnormalities, accompanied by various neurobehavioural malfunctions...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27516192/down-regulation-of-aqp4-inhibits-proliferation-migration-and-invasion-of-human-breast-cancer-cells
#18
Y-B Li, S-R Sun, X-H Han
Aquaporins (AQPs), proteinaceous water channels, have been proposed as mediators of tumour development and progression. However, the role of aquaporin 4 (AQP4), a member of the AQP family, in breast cancer has not been distinctly evaluated. The aim of the present study was to examine the effect of AQP4 down-regulation on proliferation, migration and invasion in human breast cancer. To determine this effect, siRNA interference was used to knock down its expression in T47D and MCF-7 cell lines. Down-regulation of AQP4 resulted in increased expression of E-cadherin along with an inhibitory effect on the proliferation, migration and invasion in breast cancer cells...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27516191/the-quality-control-of-mesenchymal-stromal-cells-by-in-vitro-testing-of-their-immunomodulatory-effect-on-allogeneic-lymphocytes
#19
D Lysák, L Koutová, M Holubová, T Vlas, M Miklíková, P Jindra
Mesenchymal stromal cells (MSC) represent a promising treatment of graft-versus-host disease (GVHD) in patients after allogeneic haematopoietic stem cell transplantation. We performed co-cultivation experiments with non-specifically stimulated lymphocytes to characterize the immunosuppressive activity of MSC. MSC influenced expression of some activation antigens. CD25 expression was lower with MSC and reached 55.2 % vs. 84.9 % (CD4+, P = 0.0006) and 38.8 % vs. 86.6 % (CD8+, P = 0.0003) on day +4. Conversely, CD69 antigen expression remained higher with MSC (73...
2016: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/27516190/importance-of-tumour-suppressor-gene-methylation-in-sinonasal-carcinomas
#20
M Chmelařová, I Sirák, M Mžik, K Sieglová, H Vošmiková, P Dundr, K Němejcová, J Michálek, M Vošmik, V Palička, J Laco
Epigenetic changes are considered to be a frequent event during tumour development. Hypermethylation of promoter CpG islands represents an alternative mechanism for inactivation of tumour suppressor genes, DNA repair genes, cell cycle regulators and transcription factors. The aim of this study was to investigate promoter methylation of specific genes in samples of sinonasal carcinoma by comparison with normal sinonasal tissue. To search for epigenetic events we used methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) to compare the methylation status of 64 tissue samples of sinonasal carcinomas with 19 control samples...
2016: Folia Biologica (Praha)
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