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Indian Journal of Experimental Biology

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https://www.readbyqxmd.com/read/27468469/purification-and-characterization-of-buffalo-liver-l-arginase-and-its-kinetic-properties-with-dihydropyrimidine-and-metal-ions
#1
Shivraj Hariram Nile, Se Won Park
Arginase (L-arginine amidinohydrolase, EC.3.5.3.1) from animal tissues such as, liver and kidney has been partially characterized by many researchers. In this study, we purified arginase to homogeneity from buffalo liver with about ~2857 purification fold and a 20% recovery by chromatographic and spectroscopic analysis were obtained. The molecular mass determined by gel filtration and SDS-PAGE was found to be 118 kDa and 47 kDa, respectively. The optimal pH and temperature of the arginase was 9.5 and 40°C, respectively...
June 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27468468/leucaena-leucocephala-lam-de-wit-subabul-stem-lignin-isolation-structural-characterization-and-thermal-properties
#2
Srinivasa Rao Yearla, Kollipara Padmasree
Lignin is the second most abundant renewable biopolymer on earth after cellulose. It is being used in many industrial applications due to its abundance. In the present study, lignin was isolated from the stems of Leucaena leucocephala (Lam.) de Wit., a high biomass yielding plant using acidic dioxane under N2 atmosphere. Structural characterization of isolated dioxane lignin (DL) was performed by analytical techniques: UV, FT-IR, ¹H NMR and ¹³C NMR. Their monolignol content was determined by nitrobenzene oxidation followed by HPLC-MS/MS analysis...
June 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27468467/identification-of-molecular-markers-to-study-the-garcinia-spp-diversity
#3
Utpala Parthasarathy, O P Nandakishore, O B Rosana, K Nirmal Babu, R Senthil Kumar, V A Parthasarathy
The genus Garcinia shows a considerable variation in its morphological characters such as leaf, flower and fruit with taxonomic ambiguity. It is a potential under-exploited multipurpose crop that gained considerable attention for the presence of (-) hydroxycitric acid, an anti-obesity compound, in its fruit rind and leaves. Here, we evaluated the genetic relationship through molecular markers among the selected 9 species commonly available in the Western Ghats and the Northeastern Himalayan foot hills of India...
June 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27468466/tagging-and-mapping-of-ssr-marker-for-rust-resistance-gene-in-lentil-lens-culinaris-medikus-subsp-culinaris
#4
H K Dikshit, Akanksha Singh, D Singh, M Aski, Neelu Jain, V S Hegde, A K Basandrai, D Basandrai, T R Sharma
Lentil, as an economical source of protein, minerals and vitamins, plays important role in nutritional security of the common man. Grown mainly in West Asia, North Africa (WANA) region and South Asia, it suffers from several biotic stresses such as wilt, rust, blight and broomrape. Lentil rust caused by autoecious fungus Uromyces viciae fabae (Pers.) Schroet is a serious lentil disease in Algeria, Bangladesh, Ethiopia, India, Italy, Morocco, Pakistan and Nepal. The disease symptoms are observed during flowering and early podding stages...
June 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27468465/salicylic-acid-induces-differential-antioxidant-response-in-spring-maize-under-high-temperature-stress
#5
Palak Khanna, Kamaljit Kaur, Anil K Gupta
High temperature is one of the important stress factors that affect crops in tropical countries. Plants do evolve or adopt different mechanisms to overcome such stress for survival. It is an interesting subject and has attracted many researchers to work upon. Here, we studied the effect of salicylic acid (SA) on seedling growth and antioxidative defense system in two spring maize (Zea mays L.) genotypes viz., CML-32 (relatively heat tolerant) and LM-11 (relatively heat susceptible), under high temperature stress...
June 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27468464/cytotoxic-and-pro-apoptotic-activities-of-leaf-extract-of-croton-bonplandianus-baill-against-lung-cancer-cell-line-a549
#6
J Bhavana, M K Kalaivani, A Sumathy
The acetone extract (AcE) of the Croton bonplandianus Baill., an exotic weed of the Euphorbiaceae family was studied for cytotoxicity, apoptosis, cell cycle arrest in A549 cell line and antioxidant capacities using MTT assay, acridine orange/ethidium bromide (AO/EB staining), cell cycle analysis and DPPH radical scavenging assay respectively. Based on the cytotoxic activity, the extract was tested for the apoptotic effect using AO/EB and Hoechst 33258 staining. The apoptosis was characterized by chromatin condensation and DNA fragmentation...
June 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27468463/effect-of-hydroalcoholic-fruit-extract-of-persea-americana-mill-on-high-fat-diet-induced-obesity-a-dose-response-study-in-rats
#7
Padmanabhan Monika, Arumugam Geetha
The fruits of Persea Americana Mill., commonly known as Avocado, are traditionally consumed for various health benefits including weight reduction. Here, we studied the effect of hydroalcoholic fruit extract of Persea americana (HAEPA) on high fat diet (HFD) induced obesity in rats. Obesity was induced in male Sprague Dawley rats by feeding HFD for 14 wk. The hypolipidemic effect was evaluated by co-administering 25, 50, 100 and 200 mg/kg body wt. of HAEPA. There was a significant increase in weight gain, body mass index (BMI), blood lipids, low density lipoproteins (LDL), lipid peroxides (LPO) and serum transaminases in HFD fed rats...
June 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27468462/captopril-augments-acetylcholine-induced-bronchial-smooth-muscle-contractions-in-vitro-via-kinin-dependent-mechanisms
#8
Naman Agrawal, Aparna Akella, Shripad B Deshpande
Angiotensin converting enzyme (ACE) inhibitors therapy is aassociated with bothersome dry cough as an adverse effect. The mechanisms underlying this adverse effect are not clear. Therefore, influence of captopril (an ACE inhibitor) on acetylcholine (ACh)-induced bronchial smooth muscle contractions was investigated. Further, the mechanisms underlying the captopril-induced changes were also explored. In vitro contractions of rat bronchial smooth muscle to cumulative concentrations of ACh were recorded before and after exposure to captopril...
June 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27319055/influence-of-temperature-on-symptom-expression-detection-of-host-factors-in-virus-infected-piper-nigrum-l
#9
P Umadevi, A I Bhat, K S Krishnamurthy, M Anandaraj
Expression of symptoms in black pepper plants (Piper nigrum) infected with Piper yellow mottle virus (PYMoV) vary depending on the season, being high during summer months. Here, we explored the influence of temperature on symptom expression in PYMoV infected P. nigrum. Our controlled environment study revealed increase in virus titer, total proteins, IAA and reducing sugars when exposed to temperature stress. There was change in the 2-D separated protein before and after exposure. The 2-D proteomics LC-MS identified host and viral proteins suggesting virus-host interaction during symptom expression...
May 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27319054/improvement-of-banana-cv-rasthali-silk-aab-against-fusarium-oxysporum-f-sp-cubense-vcg-0124-5-through-induced-mutagenesis-determination-of-ld50-specific-to-mutagen-explants-toxins-and-in-vitro-and-in-vivo-screening-for-fusarium-wilt-resistance
#10
M S Saraswathi, G Kannan, S Uma, R Thangavelu, S Backiyarani
Shoot tips and in vitro grown proliferating buds of banana cv. Rasthali (Silk, AAB) were treated with various concentrations and durations of chemical mutagens viz., EMS, NaN3 and DES. LD50 for shoot tips based on 50% reduction in fresh weight was determined as 2% for 3 h, 0.02% for 5 h and 0.15% for 5 h, while for proliferating buds, they were 0.6% for 30 min, 0.01% for 2 h and 0.06% for 2 h for the mutagens EMS, NaN3 and DES, respectively. Subsequently, the mutated explants were screened in vitro against fusarium wilt using selection agents like fusaric acid and culture filtrate...
May 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27319053/genetic-differentiation-and-karyotype-variation-in-hedysarum-chaiyrakanicum-an-endemic-species-of-tuva-republic-russia
#11
Natalia S Zvyagina, Olga V Dorogina, Alexander A Krasnikov
Overgrazing and mining affect vegetation, particularly in mountains. At times, it goes to such an extent that the plant species become vulnerable and slowly extinct from its habitat. Such endemic species need to be protected. One such endemic species Hedysarum chaiyrakanicum Kurbatsky, a vulnerable steppe vegetation of Tuva Republic, Russia was evaluated for its genetic diversity and taxonomic definition using molecular technique and chromosome number adjustment. The genetic differentiation among H. chaiyrakanicum, H...
May 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27319052/expression-analysis-of-bzip-transcription-factor-encoding-genes-in-response-to-water-deficit-stress-in-rice
#12
COMPARATIVE STUDY
Kishwar Ali, R D Rai, Aruna Tyagi
In plants, basic region/leucine zipper motif (bZIP) transcription factors regulate several developmental processes and activate genes in response to biotic and abiotic stresses. Role of stress responsive bZIP transcription factors was studied in paddy in relation to different stages of development and water deficit stress (WDS) in a drought tolerant cultivar N22 and susceptible IR 64. Further, relative water content (RWC), membrane stability index (MSI) and abscisic acid (ABA) content were measured as indices of WDS at different stages of development and levels of stress...
May 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27319051/pgp-potential-abiotic-stress-tolerance-and-antifungal-activity-of-azotobacter-strains-isolated-from-paddy-soils
#13
G Chennappa, M K Naik, C R Adkar-Purushothama, Y S Amaresh, M Y Sreenivasa
Azotobacter strains were isolated by serial dilution method and colonies were viscous, smooth, glistening, and brown to black colour on Jenson's N-free agar. Morphological and biochemical tests showed characteristic features of Azotobacter. Further, molecular analyses revealed the presence of different Azotobacter species viz., A. armeniacus, A. chroococcum, A. salinestris, A. tropicalis and A. vinelandii. The isolates were tested for their ability of nitrogen fixation, indole acetic acid (IAA), gibberllic acid production and phosphate solubilization...
May 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27319050/potentiation-of-antimalarial-activity-of-arteether-in-combination-with-vetiver-root-extract
#14
Sangeeta Dhawan, Sarika Gunjan, Anirban Pal, Renu Tripathi
In malaria, development of resistance towards artemisinin derivatives has urged the need for new drugs or new drug combinations to tackle the drug resistant malaria. We studied the fresh root extract of Vetiver zizanioides (Linn.) Nash (VET) with a CDRI-CIMAP antimalarial α/β arteether (ART) together for their antimalarial potential. Our results showed additive to synergistic antimalarial activity of VET and ART with sum fractional inhibitory concentrations Σ FICs 1.02 ± 0.24 and 1.12 ± 0.32 for chloroquine sensitive (CQS) and chloroquine resistant (CQR) strain of Plasmodium falciparum (William H...
May 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27319049/sertoli-cells-promote-proliferation-of-bone-marrow-derived-mesenchymal-stem-cells-in-co-culture
#15
Fenxi Zhang, Ming Lu, Hengxing Liu, Tongming Ren, Yingying Miao, Jingjing Wang
Bone marrow-derived mesenchymal stem cells (BMSCs) are a major source for cell transplantation. The proliferative ability of BMSCs is an important determinant of the efficiency of transplant therapy. Sertoli cells are "nurse" cells for development of sperm cells. Our recent study showed that Sertoli cells promoted proliferation of human umbilical cord mesenchymal stem cells (hUCMSCs) in co-culture. Studies by other groups also showed that Sertoli cells promoted growth of endothelial cells and neural stem cells...
May 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27319048/liposoluble-vitamins-in-crustacean-feed-metabolic-and-histological-responses
#16
REVIEW
Analía Verónica Fernández-Gimenez
Vitamins are vital for normal growth and survival of living organisms and they are distributed in feedstuffs in small quantities. This review is focused on the liposoluble vitamins (A, D, E and K) in the diets and metabolic responses of the Argentine penaeoid shrimps Pleoticus muelleri and Artemesia longinaris, distributed along the South American coast line. Growth, survival and histological analyses serve as indicators of the nutritional value derived from vitamin deficiency. Liposoluble vitamins are also related to stress, antioxidant defense and immune response of shrimps...
May 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27295926/dynamism-of-pgpr-in-bioremediation-and-plant-growth-promotion-in-heavy-metal-contaminated-soil
#17
P R Patel, S S Shaikh, R Z Sayyed
Heavy metal contamination, particularly of cultivable lands, is a matter of concern. Bioremediation helps in reversing such contamination to certain extent. Here, we report isolation, polyphasic identification and the role of siderophore producing rhizobacteria Alcaligenes feacalis RZS2 and Pseudomonas aeruginosa RZS3 in bioremediation of heavy metal contaminated soil and plant growth promotion activity in such contaminated soil. Siderophore produced by A. feacalis RZS2 and P. aeruginosa RZS3 strains chelated various heavy metal ions like MnCl₂...
April 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27295925/scavenging-and-antioxidant-properties-of-different-grape-cultivars-against-ionizing-radiation-induced-liver-damage-ex-vivo
#18
Indrani Singha, Subir Kumar Das
Ionizing radiation (IR) has become an integral part of the modern medicine--both for diagnosis as well as therapy. However, normal tissues or even distant cells also suffer IR-induced free radical insult. It may be more damaging in longer term than direct radiation exposure. Antioxidants provide protection against IR-induced damage. Grapes are the richest source of antioxidants. Here, we assessed the scavenging properties of four grape (Vitis vinifera) cultivars, namely Flame seedless (Black), Kishmish chorni (Black with reddish brown), Red globe (Red) and Thompson seedless mutant (Green), and also evaluated their protective action against γ-radiation-induced oxidative stress in liver tissue ex vivo...
April 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27295924/biomass-production-of-multipopulation-microalgae-in-open-air-pond-for-biofuel-potential
#19
P Selvakumar, K Umadevi
Biodiesel gains attention as it is made from renewable resources and has considerable environmental benefits. The present investigation has focused on large scale cultivation of multipopulation microalgae in open air pond using natural sea water without any additional nutritive supplements for low cost biomass production as a possible source of biofuel in large scale. Open air algal pond attained average chlorophyll concentration of 11.01 µg/L with the maximum of 43.65 µg/L as well as a higher lipid concentration of 18% (w/w) with lipid content 9...
April 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27295923/growth-response-modulation-by-putrescine-in-indian-mustard-brassica-juncea-l-under-multiple-stress
#20
Nita Lakra, Pushpa C Tomar, S N Mishra
Plants, in general, are put to various kinds of stress, biotic and abiotic, both natural and manmade. Infestation by insect pests and diseases, and extreme conditions such as salinity, temperature, etc., as well as heavy metal contamination affect their growth performance. Here, we studied the impact of salinity and heavy metal pollution on the growth performance of Indian Mustard Brassica juncea L. and its amelioration by the diamine, putrescine, a known media supplement. We evaluated the putrescine (Put) modulation potential on multiple stress effect in 7-day old Indian mustard...
April 2016: Indian Journal of Experimental Biology
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