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Chemico-biological Interactions

Xiaodong Pei, Junsong Xiao, Yumeng Zhang, Feng Lin, Zhaocheng Xiong, Guang Fu Pang, Yan Jiang, Lu Lan, Lihe Jiang
Oenothein B has a wide range of biological activities. This study assessed the possible mechanism of Oenothein B on lung cancer A549 cell. The results showed that Oenothein B effectively inhibited the proliferation of A549 cells by inducing apoptosis, arrested cell at G1 stage. On the one hand, Oenothein B not only increased the level of intracellular reactive oxygen species (ROS), but induced some apoptotic-related protein expression (cleavage caspase-3, PARP, cytochrome c level in the cytoso, Bax). Moreover, ROS inhibitor and PI3K agonist exhibited significant protection against cell death by Oenothein B...
November 16, 2018: Chemico-biological Interactions
Huan Gao, Sixi Zhang, Tingting Hu, Xiaoyu Qu, Jinghui Zhai, Yueming Zhang, Lina Tao, Jianyuan Yin, Yanqing Song
The present study assessed the therapeutic potential of omeprazole (OME), the most commonly prescribed proton pump inhibitor (PPI) used to treat gastroesophageal hyperacidity, against cisplatin (CP)-induced toxicity in human renal tubular HK-2 cells and rat kidneys. Herein, we observed that exposure of HK-2 cells to OME reversed the injury caused by CP, including enhancing cell viability and alleviating intracellular reactive oxygen species (ROS) generation and membrane damage. Concomitantly, acute exposure of male SD rats to CP induced histopathological changes, which were prevented by co-administration with OME...
November 16, 2018: Chemico-biological Interactions
Luís Pereira-de-Morais, Andressa de Alencar Silva, Renata Evaristo Rodrigues da Silva, Roger Henrique Sousa da Costa, Álefe Brito Monteiro, Cristina Rodrigues Dos Santos, Thaís de Souza Amorim, Irwin Rose Alencar de Menezes, Marta Regina Kerntopf, Roseli Barbosa
The species Lippia alba (Mill.) N. E. Brown belongs to the Verbenaceae family. It is abundant and grows spontaneously throughout the Brazilian territory. Popularly known as "erva-cidreira", it is widely used because of its sedative, carminative and analgesic properties. The objective of this study was to investigate the mechanism of action of the L. alba essential oil (EOLa) and its major constituents citral and limonene, on isolated rat uterus muscle. To evaluate the EOLa, citral and limonene effect, cumulative concentrations curves for EOLa and citral (1-600 μg/mL) and for limonene (1-1200 μg/mL) were constructed from contractions of rat uterine strips under a 1 g tension...
November 14, 2018: Chemico-biological Interactions
Chao Li, Linyan Jia, Yongsheng Yu, Liping Jin
High-risk (hr) human papillomaviruses (HPV) infection and integration has caused the majority of cervical cancer, of which E6 and E7 oncogenes are invariably retained and expressed to immortalize cells probably via affecting cell migration and invasion, and tumor metastasis. However, the underlying mechanism that mediates the procedure such as motility of cervical cancer cells within the tumor microenvironment is not well understood. Herein, we examined one possible factor-extracellular lactic acid, an end up chemical in glycolytic tumor cells, on the motility in HPV16 positive SiHa cells...
November 10, 2018: Chemico-biological Interactions
Bikram Keshari Das, Suresh K Verma, Tanushree Das, Pritam Kumar Panda, Kajal Parashar, Mrutyunjay Suar, S K S Parashar
The present study reports the regulation of cytotoxicity of Cu doped ZnO nanoparticles in macrophages (RAW 264.7) due to altered physiochemical properties changes like electrical properties by controlled doping of Cu in ZnO. Cu-doped ZnO nanoparticles were prepared by High Energy Ball Milling technique (HEBM) and formed single phase Zn1-x Cux O (x = 0.0, 0.01, 0.02, 0.03) were called as pure ZnO, Cu1%, 2%, 3% respectively. Hexagonal wurtzite structure with size range of 22-26 nm was verified. FE-SEM with EDX analysis indicated the Cu doping effect on the surface morphology of ZnO...
November 9, 2018: Chemico-biological Interactions
Ha Na Gil, Euitaek Jung, Dongsoo Koh, Yoongho Lim, Young Han Lee, Soon Young Shin
2-Hydroxy-3',5,5'-trimethoxychalcone (named DK-139) is a synthetic chalcone derivative that has anti-inflammatory, anti-tumor, and endoplasmic reticulum-mediated apoptosis activities. However, the mode of action of DK-139 on reactive oxygen species (ROS)-induced apoptosis remains unknown. In this study, we found that DK-139 activated DNA damage responses, as was revealed by the accumulation of the tumor suppressor p53 and the phosphorylation of histone H2AX at Ser139 (called γ-H2AX), which are hallmarks of DNA damage responses...
November 5, 2018: Chemico-biological Interactions
N A Soliman, W A Keshk, F H Rizk, M A Ibrahim
OBJECTIVE: Inflammatory bowel diseases (IBD) are chronic and recurrent disorders of the gastrointestinal tract with unknown etiology and have two major forms, ulcerative colitis (UC) and Crohn diseases. In view of the adverse effects and incomplete efficacy of currently administered drugs, it is essential to investigate new and harmless drugs with more desirable beneficial effects. Statins have many additional pleiotropic effects other than their lipid-lowering effect. This study aims to investigate the role of simvastatin (SIM) at different doses against induced UC in rats...
November 5, 2018: Chemico-biological Interactions
Zhu Wang, Yantian Chen, Xueyi Li, Pinky Sultana, Ming Yin, Zejian Wang
Neural stem/progenitor cell (NSPC) based therapy represents an attractive treatment for Alzheimer's disease (AD), the most common neurodegenerative disorder with no effective treatment to date. This can be achieved by stimulating endogenous NSPCs and/or administrating exogenously produced NSPCs. Successful repair requires the migration of NSPCs to the loci where neuronal loss occurs, differentiation and integration into neural networks. However, the progressive loss of neurons in the brain of AD patients suggests that the repair by endogenous NSPCs in the setting of AD may be defective...
November 3, 2018: Chemico-biological Interactions
Hidekazu Utsunomiya, Riko Hiraishi, Koji Kishimoto, Sari Hamada, Shin Abe, Yusuke Bekki, Norio Kamemura
Benzophenone-3 (BP-3) is often used as an ultraviolet (UV) light filter in sunscreen products. Although BP-3 protects the human skin and hair from damage caused by excessive UV radiation, it is reported to exhibit toxic effects in human. However, the cytotoxicity of BP-3 on various cells is still not well understood. In the present study, the cytotoxicity of BP-3 against rat thymocytes was evaluated using a flow cytometric technique with fluorescent probes. Cell mortality increased significantly after 3 h of exposure to 300 μM BP-3, whereas the mean intensity of 5-chloromethylfluorescein diacetate (5-CMF) fluorescence and cellular content of non-protein thiols decreased significantly...
November 2, 2018: Chemico-biological Interactions
Georg Menacher, Frank Balszuweit, Simon Lang, Horst Thiermann, Kai Kehe, Thomas Gudermann, Annette Schmidt, Dirk Steinritz, Tanja Popp
Although its first military use in Ypres was 100 years ago, no causal therapy for sulfur mustard (SM) intoxications exists so far. To improve the therapeutic options for the treatment of SM intoxications, we developed a co-culture of keratinocytes (HaCaT cells) and immunocompetent cells (THP-1 cells) to identify potential substances for further research. Here, we report on the influence of necrosulfonamide (NSA) on the course of a SM intoxication in vitro. The cells were challenged with 100, 200 and 300 μM SM and after 1 h treated with NSA (1, 5, 10 μM)...
November 2, 2018: Chemico-biological Interactions
Kalyani Chaubey, Syed Imteyaz Alam, Chandra Kant Waghmare, Lokendra Singh, Nalini Srivastava, Bijoy K Bhattacharya
Diisopropyl fluorophosphate (DFP), a surrogate of nerve agent sarin, is an organophosphorus (OP) compound which inhibits neuronal enzyme acetylcholinesterase (AChE). Exposure of this compound leads to a wide range of toxic symptoms and survivors may exhibit long term neurotoxicity related to cognitive and memory defects. Due to ease of availability and similar mechanism of action to other highly toxic nerve agent, DFP is widely used as model compound to trace changes associated with nerve agent exposures. Proximal fluids are widely used for the elucidation of biomarkers for exposure to toxic substances and to study the mechanism of toxicity...
October 30, 2018: Chemico-biological Interactions
Sylwia Terpilowska, Andrzej Krzysztof Siwicki
The aim of this study was to examine the effect of chromium(III), iron(III), molybdenum(III) and nickel(II) and their combinations on pro- and antioxidant activity in mouse embryo fibroblasts and liver cancer cells. The present study shows that chromium(III), iron(III), nickel(II) and molybdenum(III) induce oxidative stress. In the case of chromium(III), nickel(II) and molybdenum(III) the intracellular ROS were dominant. However, in the case of iron(III) MDA was dominant - the end product of lipid peroxidation...
October 30, 2018: Chemico-biological Interactions
Byeong-Cheol Kang, Min-Jong Kim, Soyoung Lee, Young-Ae Choi, Pil-Hoon Park, Tae-Yong Shin, Taeg Kyu Kwon, Dongwoo Khang, Sang-Hyun Kim
Mast cells play a major role in immunoglobulin E-mediated allergic inflammation, which is involved in asthma, atopic dermatitis, and allergic rhinitis. Nothofagin has been shown to ameliorate various inflammatory responses such as the septic response and vascular inflammation. In this study, we assessed the inhibitory effect of nothofagin on allergic inflammation using cultured/isolated mast cells and an anaphylaxis mouse model. Nothofagin treatment prevented histamine and β-hexosaminidase release by reducing the influx of calcium into the cytosol in a concentration-dependent manner...
October 28, 2018: Chemico-biological Interactions
Hee-Weon Lee, Suhkneung Pyo
Obesity is a critical risk factor for various diseases including type II diabetes, cerebral infarction, cardiovascular diseases, and various cancers. Acrylamide (ACR) is present in wide range of foods, including fried potato products, root vegetables, bakery products, chips, cakes, cereals, and coffee. In this study, ACR treatment dramatically increased the accumulation of lipid droplets. We also examined expression levels of peroxisome proliferator-activated receptors γ (PPARγ), CCAAT enhancer binding protein α (c/EBPα), and CCAAT enhancer binding protein β (c/EBPβ) as adipogenic transcription factors for adipocyte differentiation...
October 26, 2018: Chemico-biological Interactions
Hong Ge, Zhiguo Li, Liping Jiang, Qiujuan Li, Chengyan Geng, Xiaofeng Yao, Xiaoxia Shi, Yong Liu, Jun Cao
Hexavalent chromium [Cr (VI)], which is widely found in occupational environments, is a recognized human carcinogen. In this study, the role of endoplasmic reticulum (ER) stress in Cr (VI)-induced crosstalk of apoptosis and autophagy was investigated. Cr (VI) resulted in ER stress by upregulating the expression of GRP78 and p-PERK. 4-Phenylbutyric acid (4PBA), an inhibitor of ER stress, reduced both Cr (VI)-induced apoptosis and autophagy, suggesting that ER stress played an important role in Cr (VI)-induced apoptosis and autophagy in A549 cells...
October 25, 2018: Chemico-biological Interactions
Yi Zhang, Hui Miao, Huida Guan, Changhong Wang, Zhengtao Wang, Lili Ji
Airpotato yam is a traditional Chinese medicine used for treating thyroid disease and cancer in China. Diosbulbin B (DB) is reported to be the main hepatotoxic compound isolated from Airpotato yam. A variety of reports have shown the acute liver injury induced by DB in vivo. However, whether long-term administration of DB will cause liver fibrosis in mice is unknown. This study aims to investigate the liver fibrosis induced by long-term DB treatment in mice. C57BL/6 mice were orally given with DB (25, 50 mg/kg) for 1 or 2 month, respectively...
October 25, 2018: Chemico-biological Interactions
Huynh Nhu Mai, Sung Hoon Lee, Garima Sharma, Dae-Joong Kim, Naveen Sharma, Eun-Joo Shin, Duc Toan Pham, Quynh Dieu Trinh, Choon-Gon Jang, Seung-Yeol Nah, Ji Hoon Jeong, Hyoung-Chun Kim
We investigated whether protein kinase Cδ (PKCδ) mediates cocaine-induced hepatotoxicity in mice. Cocaine treatment (60 mg/kg, i.p.) significantly increased cleaved PKCδ expression in the liver of wild-type (WT) mice, and led to significant increases in oxidative parameters (i.e., reactive oxygen species, 4-hydroxylnonenal and protein carbonyl). These cocaine-induced oxidative burdens were attenuated by pharmacological (i.e., rottlerin) or genetic depletion of PKCδ. We also demonstrated that treatment with cocaine resulted in significant increases in nuclear factor erythroid-2-related factor 2 (Nrf-2) nuclear translocation and increased Nrf-2 DNA-binding activity in wild-type (WT) mice...
October 25, 2018: Chemico-biological Interactions
Xinxin Han, Ning Wang, Junya Li, Ying Wang, Ruiyong Wang, Junbiao Chang
Nafamostat mesilate is a serine protease inhibitor and used for the treatment of pancreatitis and cancers. The fat mass and obesity-associated protein (FTO) was identified to be a demethylase and played an important role in physiological processes. The aim of this study was to examine the inhibition of Nafamostat mesilate on FTO demethylase activity. A combination of thermodynamic and enzymatic activity studies provides an insight into the recognition of Nafamostat mesilate by FTO. The binding of Nafamostat mesilate to FTO was driven by higher positive entropy changes and smaller negative enthalpy changes...
October 25, 2018: Chemico-biological Interactions
Shengzhuang Tang, Pamela T Wong, Jayme Cannon, Kelly Yang, Sierra Bowden, Somnath Bhattacharjee, Jessica J O'Konek, Seok Ki Choi
Despite its efficacy as a skin decontaminant of reactive organophosphates (OP), Dekon 139-a potassium salt of 2,3-butanedione monooxime (DAM)-is associated with adverse events related to percutaneous absorption largely due to its small size and lipophilicity. In order to address this physicochemical issue, we synthesized and evaluated the activity of a focused library of 14 hydrophilic oxime compounds, each designed with either a DAM or monoisonitrosoacetone (MINA) oxime tethered to a polar or charged scaffold in order to optimize the size, hydrophilicity, and oxime acidity...
October 25, 2018: Chemico-biological Interactions
Ziyun Shi, Kaie She, Hong Li, Xiaohua Yuan, Xi Han, Yaqin Wang
MicroRNAs (miRNAs) are emerging as important regulators in the pathogenesis of pre-eclampsia (PE). Recent evidence has reported that miR-454 plays an important role in regulating cell proliferation and invasion. The decreased proliferation and invasion of trophoblast cells contribute to the pathogenesis of PE. However, whether miR-454 is involved in the regulation of trophoblast cell proliferation and invasion remains unknown. In this study, we aimed to investigate the potential role and underlying mechanism of miR-454 in regulating trophoblast cell proliferation and invasion in vitro...
October 24, 2018: Chemico-biological Interactions
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