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Chemico-biological Interactions

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https://www.readbyqxmd.com/read/28104348/quercetin-protects-against-heat-stroke-induced-myocardial-injury-in-male-rats-antioxidative-and-antiinflammatory-mechanisms
#1
Xiaojing Lin, Cheng-Hsien Lin, Tingbao Zhao, Dan Zuo, Zhujun Ye, Lin Liu, Mao-Tsun Lin
Heat stroke is characterilized by hyperthermia, systemic inflammation, and multiple organ failure including arterial hypotension. This definition can be fulfilled by a rat model of heat stroke used in the present study. Anesthetized animals were exposed to heat exposure (43 °C for 70 min) and then returned to room temperature (26 °C) for recovery. One hour before heat exposure, an intraperitoneal dose of quercetin (30 mg/kg) or vehicle (normal saline 1 ml/kg) was administered to the experimental groups of rats...
January 16, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28089781/genotoxic-effect-and-rat-hepatocyte-death-occurred-after-oxidative-stress-induction-and-antioxidant-gene-downregulation-caused-by-long-term-fluoride-exposure
#2
F D Campos-Pereira, L Lopes-Aguiar, F L Renosto, G A S Nogueira, E F D Costa, R Barbieri Pulz, E C M Silva-Zacarin, C A Oliveira, A A Pigoso, G D C Severi-Aguiar
Studies focusing on possible genotoxic effects of excess fluoride are contradictory and inconclusive. Currently, studies have reported a probable link to oxidative stress, DNA damage and apoptosis induced by fluoride in rat hepatocytes. We developed an in vivo study administering three doses of fluoride by gavage given to rats for 60 day. Micronucleus test was applied to investigate genotoxic potential of fluoride. The TUNEL method determined DNA fragmentation and apoptosis. Biochemical parameters to investigate mitochondrial swelling and oxidative stress...
January 12, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28088315/paris-saponin-induced-autophagy-promotes-breast-cancer-cell-apoptosis-via-the-akt-mtor-signaling-pathway
#3
Zhan-Zhi Xie, Man-Mei Li, Peng-Fei Deng, Sheng Wang, Lei Wang, Xue-Ping Lu, Liu-Bing Hu, Zui Chen, Hui-Yang Jie, Yi-Fei Wang, Xiao-Xiao Liu, Zhong Liu
Paris saponins possess anticancer, anti-inflammatory, and antiviral effects. However, the anticancer effect of Paris saponins has not been well elucidated and the mechanisms underlying the potential function of Paris saponins in cancer therapy are needed to be further identify. In this study, we report that saponin compounds isolated from Paris polyphylla exhibited antitumor activity against breast cancer cell lines, MCF-7 and MDA-MB-231. Paris saponin XA-2 induced apoptosis in both cell lines, as evidenced by the activation of caspases and cleavage of Poly (ADP-ribose) polymerase...
January 11, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28087463/effect-of-22-cyp2d6-variants-found-in-the-chinese-population-on-tolterodine-metabolism-in-vitro
#4
Hao Wang, Da-Peng Dai, Peng Sun, Li-Ping Xu, Bing-Qing Liang, Jian-Ping Cai, Guo-Xin Hu
Cytochrome P450 2D6 (CYP2D6) is an important member of the cytochrome P450 enzyme superfamily. We recently identified 22 novel variants in the Chinese population using PCR and bidirectional sequencing methods. The aim of this study is to characterize the enzymatic activity of these variants and their effects on the metabolism of the antimuscarinic drug tolterodine in vitro. A baculovirus-mediated expression system was used to express wild-type CYP2D6 and 24 variants (CYP2D6*2, CYP2D6*10, and 22 novel CYP2D6 variants) at high levels...
January 10, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28087462/impact-of-disease-linked-mutations-targeting-the-oligomerization-interfaces-of-aldehyde-dehydrogenase-7a1
#5
David A Korasick, John J Tanner, Michael T Henzl
Aldehyde dehydrogenase 7A1 (ALDH7A1) is involved in lysine catabolism, catalyzing the oxidation of α-aminoadipate semialdehyde to α-aminoadipate. Certain mutations in the ALDH7A1 gene, which are presumed to reduce catalytic activity, cause an autosomal recessive seizure disorder known as pyridoxine-dependent epilepsy (PDE). Although the genetic association between ALDH7A1 and PDE is well established, little is known about the impact of PDE-mutations on the structure and catalytic function of the enzyme. Herein we report the first study of the molecular consequences of PDE mutations using purified ALDH7A1 variants...
January 10, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28087461/low-glucose-stress-decreases-cellular-nadh-and-mitochondrial-atp-in-colonic-epithelial-cancer-cells-influence-of-mitochondrial-substrates
#6
Magdalena L Circu, Ronald E Maloney, Tak Yee Aw
In this study, we investigated how colonic epithelial cells maintained pyridine nucleotide (NADH/NAD(+)) redox homeostasis upon acute metabolic variation imposed by glucose deprivation or supplementation with mitochondrial substrates, succinate and malate/glutamate (M/G). Our results showed that low glucose caused cellular NADH/NAD(+) redox imbalance that diminished lactate dehydrogenase (LDH) activity and resulted in lower lactate contents. The concurrent activation of malic enzyme (ME) suggested a role for malate in preserving cellular pyruvate that remained unchanged at low glucose...
January 10, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28040552/chemoprotective-role-of-quercetin-in-manganese-induced-toxicity-along-the-brain-pituitary-testicular-axis-in-rats
#7
Isaac A Adedara, Temitayo I Subair, Valerie C Ego, Oluwasetemi Oyediran, Ebenezer O Farombi
Reproductive dysfunction in response to manganese exposure has been reported in humans and animals. Quercetin, a bioflavonoid widely distributed in fruits, vegetables and beverages has been shown to possess antioxidant, anti-inflammatory and anti-apoptotic activities in different experimental model systems. However, there is dearth of scientific information on the influence of quercetin on manganese-induced reproductive toxicity. This study was designed to evaluate the influence of quercetin on manganese-induced functional alterations along the brain-pituitary- testicular axis in rats...
December 28, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28038895/corneal-haze-phenotype-in-aldh3a1-null-mice-in-vivo-confocal-microscopy-and-tissue-imaging-mass-spectrometry
#8
Ying Chen, James V Jester, David M Anderson, Satori A Marchitti, Kevin L Schey, David C Thompson, Vasilis Vasiliou
ALDH3A1 is a corneal crystallin that protects ocular tissues from ultraviolet radiation through catalytic and non-catalytic functions. In addition, ALDH3A1 plays a functional role in corneal epithelial homeostasis by simultaneously modulating proliferation and differentiation. We have previously shown that Aldh3a1 knockout mice in a C57B6/129sV mixed genetic background develop lens cataracts. In the current study, we evaluated the corneal phenotype of Aldh3a1 knockout mice bred into a C57B/6J congenic background (KO)...
December 27, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28038894/mechanistic-characterization-and-inhibition-of-sphingomyelinase-c-over-substituted-iron-schiff-bases-of-chitosan-adsorbed-on-glassy-carbon-electrode
#9
Claudia A Caro, Luis Lillo, Francisco J Valenzuela, Gerardo Cabello
The medical treatment of laxoscelisms is based solely on supportive measures. Although equine antiserum for Sphingomyelinase C (SMASE) and D isomers are available, it is not used due to the risk of an anaphylactic reaction and its unproven efficacy. As potential enzyme inhibitors, derivatives of Iron chitosan complexes were studied (Shiff base having -R = -H, -Cl, -Br, -F, -OCH3, -CH3, -NO2). These chitosan complexes were chosen because they have revealed good results in medicine and catalysis due to their biodegradable characteristics and bioavailability...
December 27, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28027877/hydrogen-sulfide-may-attenuate-methylmercury-induced-neurotoxicity-via-mitochondrial-preservation
#10
Junyan Han, Xuelian Yang, Xuehua Chen, Zhiqiang Li, Ming Fang, Bing Bai, Dehong Tan
Hydrogen sulfide (H2S) is a protective molecule and a novel gaseous mediator. Here we explored whether H2S donor (NaHS) could attenuate methylmercury (MeHg)-induced neurotoxicity in rats. The adult rats were randomly divided into four groups, i.e., control, NaHS, MeHg, and NaHS + MeHg groups. Rats of the NaHS + MeHg group were intraperitoneally (i.p) injected with 5.6 mg/kg/d of NaHS together with 5 μg/kg/d of MeHg. Rats of the MeHg group and NaHS group were injected with 5 μg/kg/d of MeHg and 5.6 mg/kg/d of NaHS, respectively...
December 24, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28027876/ercc2-xpd-lys751gln-alter-dna-repair-efficiency-of-platinum-induced-dna-damage-through-p53-pathway
#11
Guopei Zhang, Yangyang Guan, Yuejiao Zhao, Tahar van der Straaten, Sha Xiao, Ping Xue, Guolian Zhu, Qiufang Liu, Yuan Cai, Cuihong Jin, Jinghua Yang, Shengwen Wu, Xiaobo Lu
Platinum-based treatment causes Pt-DNA adducts which lead to cell death. The platinum-induced DNA damage is recognized and repaired by the nucleotide excision repair (NER) system of which ERCC2/XPD is a critical enzyme. Single nucleotide polymorphisms in ERCC2/XPD have been found to be associated with platinum resistance. The aim of the present study was to investigate whether ERCC2/XPD Lys751Gln (rs13181) polymorphism is causally related to DNA repair capacity of platinum-induced DNA damage. First, cDNA clones expressing different genotypes of the polymorphism was transfected to an ERCC2/XPD defective CHO cell line (UV5)...
December 24, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28025170/instability-of-c154y-variant-of-aldo-keto-reductase-1c3
#12
Satoshi Endo, Sayaka Takada, Ryo P Honda, Kathrin Müller, Jochen H Weishaupt, Peter M Andersen, Albert C Ludolph, Yuji O Kamatari, Toshiyuki Matsunaga, Kazuo Kuwata, Ossama El-Kabbani, Akira Ikari
Aldo-keto reductase (AKR) 1C3 is a cytosolic enzyme that metabolizes steroids, prostaglandins, toxic aldehydes and drugs. Recently, some nonsynonymous single nucleotide polymorphisms of AKR1C3 have been suggested to impact steroid and drug metabolism. In this study, we examined the effects of C154Y and L159V variants of AKR1C3 on stability and function of the enzyme. Both variants had been detected in patients with the neurodegenerative disease amyotrophic lateral sclerosis. Recombinant wild-type (WT), C154Y and L159V enzymes were similar in specific activity, but C154Y displayed much lower thermostability than WT and L159V...
December 23, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28025169/evaluation-of-in%C3%A2-vitro-toxicity-of-polymeric-micelles-to-human-endothelial-cells-under-different-conditions
#13
Fang Liu, Haikang Huang, Yu Gong, Juan Li, Xuefei Zhang, Yi Cao
Polymeric micelles have been extensively studied in the area of antitumor therapy, and more recently explored as nanocarriers for atherosclerosis. These applications of polymeric micelles in biomedicine will increase their contact with human blood vessels. However, few studies have considered the interactions between polymeric micelles and endothelial cells, especially in a complex system. This study used human umbilical vein endothelial cells (HUVECs) as an in vitro model for endothelial cells to investigate the toxic effects of methoxy-poly(ethylene glycol)-poly(d,l-lactide) (MPEG-PLA) based micelles...
December 23, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28025168/inversion-of-substrate-stereoselectivity-of-horse-liver-alcohol-dehydrogenase-by-substitutions-of-ser-48-and-phe-93
#14
Keehyuk Kim, Bryce V Plapp
The substrate specificities of alcohol dehydrogenases (ADH) are of continuing interest for understanding the physiological functions of these enzymes. Ser-48 and Phe-93 have been identified as important residues in the substrate binding sites of ADHs, but more comprehensive structural and kinetic studies are required. The S48T substitution in horse ADH1E has small effects on kinetic constants and catalytic efficiency (V/Km) with ethanol, but decreases activity with benzyl alcohol and affinity for 2,2,2-trifluoroethanol (TFE) and 2,3,4,5,6-pentafluorobenzyl alcohol (PFB)...
December 23, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28012710/potential-anticancer-activity-of-lichen-secondary-metabolite-physodic-acid
#15
V Cardile, A C E Graziano, R Avola, M Piovano, A Russo
Secondary metabolites present in lichens, which comprise aliphatic, cycloaliphatic, aromatic and terpenic compounds, are unique with respect to those of higher plants and show interesting biological and pharmacological activities. However, only a few of these compounds, have been assessed for their effectiveness against various in vitro cancer models. In the present study, we investigated the cytotoxicity of three lichen secondary metabolites (atranorin, gyrophoric acid and physodic acid) on A375 melanoma cancer cell line...
December 21, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27989598/effect-of-trans-anethole-limonene-and-your-combination-in-nutritional-components-and-their-reflection-on-reproductive-parameters-and-testicular-apoptosis-in-spodoptera-frugiperda-lepidoptera-noctuidae
#16
Glaucilane S Cruz, Valéria Wanderley-Teixeira, José V Oliveira, Carolline G D' Assunção, Franklin M Cunha, Álvaro A C Teixeira, Carolina A Guedes, Kamilla A Dutra, Douglas R S Barbosa, Mariana O Breda
Spodoptera frugiperda is a major pest in several crops due to its polyphagous habit. Studies on the use of essential oils for pest control have been increasing over the years, presenting itself as a promising alternative with less environmental impact. Chemical profile evaluations of essential oils enable the knowledge and use of major compounds, providing a better understanding of their actions in the life history of insects. Thus, the study evaluated the effects of the major compounds limonene, trans-anethole and the combined effects upon nutrition, reproduction and testicular apoptosis in S...
December 16, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27989597/comparative-and-evolutionary-studies-of-aldh18a1-genes-and-proteins
#17
Roger S Holmes
Vertebrate ALDH18A1 genes encode a bifunctional mitochondrial enzyme, catalyzing a 2-step conversion of glutamate to glutamyl semialdehyde, subsequently converted into proline, ornithine and arginine. Bioinformatic analyses of vertebrate and invertebrate genomes were undertaken using known ALDH18A1 amino acid sequences. G5K (glutamyl kinase) and GPR (glutamyl phosphate reductase) domain sequences were identified for all vertebrate and invertebrate genomes examined, whereas bacterial sequences encoded separate enzymes...
December 16, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27989600/novel-ferrocenyl-pyrazoles-inhibit-breast-cancer-cell-viability-via-induction-of-apoptosis-and-inhibition-of-pi3k-akt-and-erk1-2-signaling
#18
Harika Atmaca, Ayşe Nur Özkan, Metin Zora
Despite the advances in early detection and targeted therapies, chemotherapy is still of vital importance in breast cancer treatment. However, development of drug resistance and serious side effects limits their usage. Thus, there is an urgent need for safer and more effective agents against breast cancer. We have previously described the synthesis of a number of pyrazole derivatives, and in the current study, we have investigated the effects of two different ferrocenyl pyrazole (FP) derivates, 5-ferrocenyl-1-phenyl-1H-pyrazole (FP-Ph) and 5-ferrocenyl-1H-pyrazole (FP-H), on breast cancer cells...
December 15, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27989599/lycopene-inhibits-reactive-oxygen-species-production-in-sk-hep-1%C3%A2-cells-and-attenuates-acetaminophen-induced-liver-injury-in-c57bl-6-mice
#19
Ana Carla Balthar Bandeira, Talita Prato da Silva, Glaucy Rodrigues de Araujo, Carolina Morais Araujo, Rafaella Cecília da Silva, Wanderson Geraldo Lima, Frank Silva Bezerra, Daniela Caldeira Costa
Our aim was to investigate the antioxidant potential of lycopene in different experimental liver models: in vitro, to evaluate the influence of lycopene on reactive oxygen species (ROS) production mediated by the PKC pathway and in vivo, to evaluate the protective effects of lycopene in an experimental model of hepatotoxicity. The in vitro study assessed the lycopene antioxidant potential by the quantification of ROS production in SK-Hep-1 cells unstimulated or stimulated by an activator of the PKC pathway...
December 15, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27986436/interaction-of-plant-essential-oil-terpenoids-with-the-southern-cattle-tick-tyramine-receptor-a-potential-biopesticide-target
#20
Aaron D Gross, Kevin B Temeyer, Tim A Day, Adalberto A Pérez de León, Michael J Kimber, Joel R Coats
An outbreak of the southern cattle tick, Rhipicephalus (Boophilus) microplus, (Canestrini), in the United States would have devastating consequences on the cattle industry. Tick populations have developed resistance to current acaricides, highlighting the need to identify new biochemical targets along with new chemistry. Furthermore, acaricide resistance could further hamper control of tick populations during an outbreak. Botanically-based compounds may provide a safe alternative for efficacious control of the southern cattle tick...
December 14, 2016: Chemico-biological Interactions
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