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Journal of Medical Microbiology

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https://www.readbyqxmd.com/read/28109201/genetic-analyses-of-the-fusion-protein-genes-in-human-parainfluenza-virus-types-1-and-3-among-patients-with-acute-respiratory-infections-in-eastern-japan-from-2011-to-2015
#1
Rika Tsutsui, Hiroyuki Tsukagoshi, Koo Nagasawa, Masaki Takahashi, Yuki Matsushima, Akihide Ryo, Makoto Kuroda, Hideki Takami, Hirokazu Kimura
PURPOSE: To genetically explorer the fusion protein gene (F) in human parainfluenza virus type 1 (HPIV1) and type 3 (HPIV3) strains, we analyzed them in the patients with acute respiratory infections (ARI) in Eastern Japan from 2011 to 2015. METHODOLOGY: We constructed phylogenetic trees based on the HPIV and HPIV3 F gene using the maximum likelihood method and conducted p-distance, and selective pressure analyses. We also predicted the linear epitopes of the protein in the prototype strains...
January 20, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28100314/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-rapid-identification-of-fungal-rhinosinusitis-pathogens
#2
Xinxin Lu, Yanfei Huang, Mingxin Zhang, Min Zhu, Mei Wang, Yufeng Sun, Haitong Gu, Jingjing Cao, Xue Li, Shaoya Zhang, Jinglin Wang
BACKGROUND: filamentous fungi are the most important pathogens causing fungal rhinosinusitis (FRS). Current laboratory diagnosis of FRS pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time-consuming and expertise-dependent. Although matrix -assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been employed to identify various fungi, its performance for the identification of FRS fungi is less clear...
January 17, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28100300/molecular-analysis-and-species-diversity-of-nocardia-in-hospital-environment-of-a-developing-country-a-potential-health-hazard
#3
Hossein Ali Rahdar, Davood Azadi, Hasan Shojaei, Abbas Daei Naser
BACKGROUND: Despite hundreds of reports on the isolation of Nocardia from clinical samples, the presence and diversity of Nocardia species capable of survival in a harsh and adverse condition such as hospital environment have not been comprehensively studied. The aim of this study was to assess the Nocardia species diversity in the hospital environment to provide a better insight on their potential threat as a reservoir for the development of nosocomial infections. MATERIALS AND METHODS: A total of 90 hospital water, dust and soil samples collected from 30 hospitals were analyzed for the presence of Nocardia using standard protocols for isolation and characterization of the isolates...
January 17, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28100311/the-evaluation-of-three-different-bottles-in-bactec-9240-automated-blood-culture-system-and-direct-identification-of-candida-species-to-shorten-the-turnaround-time-of-blood-culture
#4
Egemen Gokbolat, Yasemin Oz, Selma Metintas
Candida spp. are the most common causes of fungemia. Rapid and accurate diagnostic methods are very important for appropriate management of candidemia. At present, blood culture is the essential diagnostic test despite it has a long detection time and low sensitivity rate. We aimed to investigate the ways to shorten the turnaround time from blood culture collection to final identification in candidemia. Sixty clinical bloodstream isolates of Candida were included and the Plus Aerobic/F, Peds Plus/F and Mycosis IC/F (MICF) bottles were used with BACTEC 9240 blood culture instrument...
January 16, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28086079/a-large-prolonged-outbreak-of-hepatitis-a-associated-with-consumption-of-frozen-berries-italy-2013-14
#5
Gaia Scavia, Valeria Alfonsi, Stefania Taffon, Martina Escher, Roberto Bruni, Dario De Medici, Simona Di Pasquale, Sarah Guizzardi, Benedetta Cappelletti, Stefania Iannazzo, Nadia Marina Losio, Enrico Pavoni, Lucia Decastelli, Anna Rita Ciccaglione, Michele Equestre, Maria Elena Tosti, Caterina Rizzo
In 2013/2014 Italy experienced one of the largest community-wide prolonged outbreak of Hepatitis A virus (HAV) through over the country. Enhanced human epidemiological and microbiological surveillance together with microbiological monitoring of HAV in food and trace-back investigation were conducted in order to give a comprehensive description of the outbreak investigation carried out in Italy by a multidisciplinary National Task Force in collaboration with regional and local public health authorities. Moreover, control strategies of foodborne HAV infection in both the human and food sectors are described...
January 13, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28086078/role-and-mechanism-of-the-hsp70-molecular-chaperone-machines-in-bacterial-pathogens
#6
Ciamak- Ghazaei
Heat shock proteins (HSP) are highly conserved, stress-inducible, ubiquitous proteins that maintain homeostasis in both eukaryotes and prokaryotes. Hsp70 proteins belong to the HSP family and enhance bacterial survival in hostile environments. Hsp70, known as DnaK in prokaryotes, supports numerous processes such as the assembly and disassembly of protein complexes, the refolding of misfolded and clustered proteins, membrane translocation, and the regulation of regulatory proteins. The chaperone-based activity of Hsp70 depends on dynamic interactions between its two domains known as the ATPase domain and the substrate binding domain...
January 12, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28086073/evolutionary-and-genetic-analysis-of-human-bocavirus-genotype-1-strains-reveals-an-evidence-of-intragenomic-recombination
#7
Ahmed S Abdel-Moneim, Mahmoud M Kamel, Naglaa M Hassan
Human bocavirus (HBoV) is divided into 4 genotypes: 1 to 4 with the HBoV-1 is the most commonly prevalent genotype. The current study intended to genetically analyze the full length genome of the HBoV-1 of recently detected Egyptian strains. Seven overlapping sets of primers were developed to amplify a nearly complete HBoV-1 genome from the clinical samples. The primer sets were tested on three recently identified Egyptian HBoV-1 strains with viral loads ≥105/ml. Sequencing was conducted using the same sets of primers...
January 11, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28086068/improved-effect-of-amikacin-loaded-poly-d-l-lactide-co-glycolide-plga-nanoparticles-against-planktonic-and-biofilm-cells-of-pseudomonas-aeruginosa
#8
Parastoo Sabaeifard, Ahya Abdi-Ali, Carlos Gamazo, Juan M Irache, Mohammad Reza Soudi
Amikacin is one of the most effective antibiotics against Pseudomonas aeruginosa infections but, according to its high toxicity, the use of this antibiotic has been clinically limited. In the present study, amikacin was successfully loaded into a new formulation of nanoparticles based on PLGA 50:50. Amikacin-loaded nanoparticles showed desirable antibacterial and antibiofilm characteristics. Flow cytometric studies showed that these nanoparticles were able to interact with Planktonic and biofilm bacterial cells...
January 11, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28068218/comparison-of-self-collected-meatal-swabs-with-urine-specimens-for-the-diagnosis-of-chlamydia-trachomatis-and-neisseria-gonorrhoeae-in-men
#9
Lisa Berry, Belinda Stanley
Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) are common bacterial causes of sexually transmitted infection. Self-taken meatal swabs are a possible alternative to urine samples in testing for infection; however the data surrounding their use are limited. We carried out a prospective service review in a large sexual health clinic comparing urine samples and self-taken meatal swabs in men presenting for sexual transmissible infection screening for CT and GC with the BD Viper XTR system. We found an overall prevalence of CT infections was 10...
January 9, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28068217/manual-curation-and-reannotation-of-the-genomes-of-clostridium-difficile-630%C3%AE-erm-and-clostridium-difficile-630
#10
Henning Dannheim, Thomas Riedel, Meina Neumann-Schaal, Boyke Bunk, Isabel Schober, Cathrin Spröer, Cynthia Maria Chibani, Sabine Gronow, Heiko Liesegang, Jörg Overmann, Dietmar Schomburg
We resequenced the genome of Clostridium difficile 630Δerm (DSM 28645), a model strain commonly used for the generation of insertion mutants. The genome sequence was obtained by a combination of single-molecule real-time (SMRT) and Illumina sequencing technology. Detailed manual curation and comparison to the previously published genomic sequence revealed sequence differences including inverted regions and the presence of plasmid pCD630. Manual curation of our previously deposited genome sequence of the parental strain 630 (DSM 27543) led to an improved genome sequence...
January 9, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28068213/utilization-of-size-polymorphism-in-its1-and-its2-regions-for-identification-of-pathogenic-yeast-species
#11
Hossein Khodadadi, Ladan Karimi, Niloufar Jalali-Zand, Hassan Adin, Hossein Mirhendi
Despite the existence of a variety of available yeast identification strategies, easier and more cost-effective methods are required for routine use in clinical laboratories. The internal transcribed spacer (ITS) regions of fungal rRNA genes exhibit variable sizes depending on the yeast species. In the present study, fragment size polymorphism (FSP) analysis of the ITS1 and ITS2 regions for identification of the clinically most important yeast species was assessed. The ITS1 and ITS2 regions of 190 strains, including isolates of 31 standard strains and 159 clinical isolates, were separately PCR-amplified with two primer sets: ITS1-ITS2 and ITS3-ITS4...
January 9, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28056227/detection-and-dissemination-of-the-colistin-resistance-gene-mcr-1-from-isolates-and-fecal-samples-in-china
#12
Xia Chen, Xiaofei Zhao, Jie Che, Yanwen Xiong, Yanmei Xu, Lifeng Zhang, Ruiting Lan, Lining Xia, Timothy R Walsh, Jianguo Xu, Jinxing Lu, Juan Li
A recently identified colistin resistance gene, mcr-1, has been reported in many countries. In this study, we established a new real-time PCR method to detect the mcr-1 gene in a variety of isolates and fecal samples from 20 provinces and municipal cities in China. Of the 2330 isolates (from 10 species) screened, 54 (2.3%) isolates were positive for mcr-1. All of the mcr-1-positive isolates that were identified belonged to Escherichia coli (E. coli) strains, among which 9, 1, and 44 were identified as enteropathogenic E...
January 5, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28056222/in-vitro-activity-of-antimicrobial-agents-against-multidrug-and-extensively-drug-resistant-acinetobacter-baumannii
#13
Francielli Mahnic Vasconcellos, Monique Ribeiro Tiba Casas, Lais Calissi Brisolla Tavares, Doroti Oliveira Garcia, Carlos Henrique Camargo
Multidrug- (MDR) and extensively drug-resistant (XDR) Acinetobacter baumannii (Ab) difficult the treatment of severe infections. We evaluated the in vitro activity of antimicrobial agents against 71 carbapenem-resistant clonal diverse clinical Ab strains recovered from Brazilian hospitals. Minocycline and polypeptides presented activity against >98% of the isolates, indicating their potential against MDR or XDR carbapenem-resistant Acinetobacter baumannii from main clones circulating in Brazil.
January 5, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28056219/antimicrobial-resistant-escherichia-coli-and-enterococcus-spp-isolated-from-miranda-donkey-equus-asinus-an-old-problem-from-a-new-source-with-a-different-approach
#14
Isabel Carvalho, Rosa Del Campo, Margarida Sousa, Nuno Silva, João Carrola, Catarina Marinho, Tiago Santos, Silvia Carvalho, Miguel Nóvoa, Miguel Quaresma, José Eduardo Pereira, Marta Cobo, Gilberto Igrejas, Patricia Poeta
The Miranda Donkey (Equus asinus) is an endangered asinine from Miranda do Douro region, located in North East of Portugal. We study the antimicrobial resistance (AMR) and virulence genes in Escherichia coli and Enterococcus spp. isolates from these animals. In March 2014, a total of 66 faecal samples were recovered from independent animals. Antibiotic resistance was determined by the disk diffusion method. Carriage of genes codifying for antibiotic resistant and virulent factors was analyzed by polymerase chain reaction (PCR)...
January 5, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28051952/antimicrobial-susceptibility-of-gram-negative-eskape-pathogens-isolated-from-hospitalized-patients-with-intra-abdominal-and-urinary-tract-infections-in-asia-pacific-countries-smart-2013-2015
#15
James A Karlowsky, Daryl J Hoban, Meredith A Hackel, Sibylle Lob, Daniel F Sahm
Gram-negative ESKAPE pathogens (Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) are responsible for increases in antimicrobial-resistant infections worldwide. We determined in vitro susceptibilities to eight parenteral antimicrobial agents using CLSI broth microdilution methodology for gram-negative ESKAPE pathogens isolated from hospitalized patients with intra-abdominal infections (IAI) (n = 3,052) and urinary tract infections (UTI) (n = 1,088) in 11 countries/regions from 2013-2015...
January 3, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28051951/emergence-of-a-novel-%C3%AE-lactamase-in-food-of-animal-origin-characterisation-of-shv-111-from-a-cheese-isolate-of-klebsiella-pneumoniae-with-an-amino-acid-substitution-in-a-strictly-conserved-position-p174s-in-the-%C3%AE-loop
#16
https://www.readbyqxmd.com/read/28051950/phylogenetic-analysis-of-partial-vp7-gene-of-the-emerging-human-group-a-rotavirus-g12-strains-circulating-in-tunisia
#17
Amal Moussa, Mouna Ben Hadj Fredj, Meriam BenHamida-Rebaï, Imene Fodha, Noureddine Boujaafar, Abdelhalim Trabelsi
Group A rotavirus (RVA) is the leading etiological agent of severe gastroenteritis in children less than 5 years of age. The most common G types in humans are G1-4 and G9. G12 genotype is currently emerging worldwide, becoming the sixth most prevalent RVA VP7 genotype causing severe childhood diarrhea. A total of 1127 fecal samples were collected from Tunisian children under 5 years of age consulting for gastroenteritis between 2009 and 2014. All samples were screened by enzyme-linked immunosorbent assay for the presence of RVA antigen...
January 3, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28032543/candida-albicans-biofilm-heterogeneity-does-not-influence-denture-stomatitis-but-strongly-influences-denture-cleansing-capacity
#18
Lindsay E O'Donnell, Hasanain K A Alalwan, Ryan Kean, Gareth Calvert, Christopher J Nile, David F Lappin, Douglas Roberston, Craig Williams, Gordon Ramage, Leighann Sherry
Approximately 20% of the UK population wear some form of denture prosthesis, resulting in denture stomatitis in half of these individuals. Candida albicans is primarily attributed as the causative agent, due to its biofilm forming ability. Recently, there has been increasing evidence of C. albicans biofilm heterogeneity and the negative impact it can have clinically; however, this phenomenon has yet to be studied in relation to denture isolates. The aims of this study were to evaluate C. albicans biofilm formation of clinical denture isolates in a denture environment, and assess antimicrobial activity of common denture cleansers against these tenacious communities...
December 29, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28032544/molecular-epidemiology-of-noroviruses-detected-in-vietnamese-children-with-acute-gastroenteritis-from-2012-to-2015
#19
Thi Nguyen Hoa-Tran, Osamu Nakagomi, Anh Thi Hai Dao, Anh The Nguyen, Chantal Ama Agbemabiese, Hung Manh Vu, Toyoko Nakagomi, Thanh Thi Hien Nguyen
Noroviruses, an important cause of diarrhoea in humans, are genetically diverse. The recent norovirus seasons recorded the emergence of new recombinants of the capsid and polymerase genotypes, with a global dominance of GII.Pe_GII.4 Sydney 2012 and GII.P17_GII.17 in Asian countries. However, the number of papers reporting the distribution of both polymerase and capsid genotypes circulating among children is scarce with none from Vietnam. This study described both the polymerase and capsid genotypes of noroviruses circulating in Vietnamese children using stool specimens obtained under the World Health Organisation rotavirus surveillance programme from 2012 to 2015...
December 28, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28032542/the-molecular-epidemiology-and-antimicrobial-resistance-phenotypes-of-acinetobacter-baumannii-isolated-from-patients-in-three-hospitals-in-southern-vietnam
#20
Nguyen Tuan Anh, Tran Vu Thieu Nga, Huynh Minh Tuan, Nguyen Si Tuan, Dao Minh Y, Nguyen Van Vinh Chau, Stephen Baker, Ho Huynh Thuy Duong
Multidrug resistance (MDR) in the nosocomial pathogen Acinetobacter baumannii limits therapeutic options and impacts on clinical care. Resistance against carbapenems, a group of last-resort antimicrobials for treating MDR A. baumannii infections, is associated with the expression (and over-expression) of carbapenemases encoded by the blaOXA genes. The aim of this study was to determine the prevalence of antimicrobial resistant A. baumannii associated with infection in three hospitals in southern Vietnam and to characterise the genetic determinants associated with resistance against carbapenems...
December 28, 2016: Journal of Medical Microbiology
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