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Journal of Medical Microbiology

Duranti Anna, Sabbatucci Michela, Blasi Giuliana, Acciari Vicdalia Aniela, Ancora Massimo, Bella Antonino, Busani Luca, Centorame Patrizia, Cammà Cesare, Conti Fabrizio, De Medici Dario, Di Domenico Marco, Di Marzio Violeta, Filippini Giovanni, Fiore Alfonsina, Fisichella Stefano, Gattuso Antonietta, Gianfranceschi Monica, Graziani Caterina, Guidi Fabrizia, Marcacci Maurilia, Marfoglia Cristina, Neri Diana, Orsini Massimiliano, Ottaviani Donatella, Petruzzelli Annalisa, Pezzotti Patrizio, Rizzo Caterina, Ruolo Anna, Scavia Gaia, Scuota Stefania, Tagliavento Giuliano, Tibaldi Alberto, Tonucci Francesco, Torresi Marina, Migliorati Giacomo, Pomilio Francesco
PURPOSE: From May 2015 to March 2016, an outbreak due to Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred in central Italy, involving 24 confirmed clinical cases. The article provides a description of the outbreak and the investigation carried out by a multidisciplinary network. METHODOLOGY: Epidemiological and microbiological surveillance was conducted to confirm the outbreak and to detect the food vehicle of infection...
July 19, 2018: Journal of Medical Microbiology
G G T Nisansala, D Muthusinghe, T D C P Gunasekara, M M Weerasekera, S S N Fernando, K N P Ranasinghe, M G C P Marasinghe, P S Fernando, N Koizumi, C D Gamage
Leptospirosis is an endemic infectious disease causing considerable morbidity and mortality in Sri Lanka; however, reports on the isolation of Leptospira from infected patients in Sri Lanka have been largely unavailable since the 1970s. Two isolates were obtained and characterized from 100 blood cultures from leptospirosis-suspected patients. Phylogenic analysis of partial flaB gene sequences identified the isolates as Leptospira interrogans. The patient serum samples from which Leptospira was isolated reacted with the Leptospira serogroups Sejroe and Canicola at a titre of 1 : 200...
July 19, 2018: Journal of Medical Microbiology
Suat Moi Puah, Wei Ching Khor, Boon Pin Kee, Jin Ai Mary Anne Tan, Savithri D Puthucheary, Kek Heng Chua
PURPOSE: The taxonomy of Aeromonas keeps expanding and their identification remains problematic due to their phenotypic and genotypic heterogeneity. In this study, we aimed to develop a rapid and reliable polymerase chain reaction-restriction fragment length polymorphism assay targeting the rpoD gene to enable the differentiation of aeromonads into 27 distinct species using microfluidic capillary electrophoresis. METHODOLOGY: A pair of degenerate primers (Aero F: 5'-YGARATCGAYATCGCCAARCGB-3' and Aero R: 5'-GRCCDATGCTCATRCGRCGGTT-3') was designed that amplified the rpoD gene of 27 Aeromonas species...
July 19, 2018: Journal of Medical Microbiology
GeeHay Hong, Yun Guk Park, Han Wool Jin, Sam Kyu Choi, Hyun Young Chi, Woo Jae Cho, Joo Hee Lee, Sol Han, Sun Pyo Hong
PURPOSE: Human papillomavirus (HPV) infection in women is known to promote the development of cervical neoplasia. Specific HPV genotypes are more highly associated with disease, and therefore detection and genotyping of HPV infection is critical for preventing and effectively treating cervical cancer. Consequently, various assays using diverse technologies have been developed to detect HPV genotype. Recently the OmniPlex-HPV and GeneFinder HPV methods, based on PCR and Luminex xMAP liquid bead microarray technologies, were developed for the detection of 40 and 32 HPV genotypes, respectively...
July 17, 2018: Journal of Medical Microbiology
Maryam Homayoon, Yahya Tahamtan, Mohammad Kargar, Seyed Mohammad Hossein Hosseini, Abbas Akhavan Sepahy
PURPOSE: Pasteurella multocida (P. multocida) is a principal pathogen of domestic animals and an opportunistic pathogen of humans. It is the causative agent of pneumonia and haemorrhagic septicaemia in cattle, sheep and goats, fowl cholera in chickens and progressive atrophic rhinitis in swine. In this study, we investigated the humoral and cellular immune responses and protective immunity conferred by an iron-inactivated vaccine with bacterial DNA (IIV+bDNA) as an adjuvant in mice. METHODOLOGY: P...
July 17, 2018: Journal of Medical Microbiology
Vincent Mathy, Patrick Grohs, Fabrice Compain
The in vitro activity of anti-pseudomonal β-lactams in combination with avibactam was evaluated against 54 multidrug-resistant non-fermenting Gram-negative bacilli isolated from cystic fibrosis patients. Avibactam increased and/or restored the antibacterial activities of ceftazidime and aztreonam against Pseudomonas aeruginosa and Stenotrophomonas maltophilia, respectively. No β-lactam-avibactam combination was active against Achromobacter xylosoxidans.
July 17, 2018: Journal of Medical Microbiology
Nithin Sam Ravi, Shalini Anandan, Saranya Vijayakumar, Radha Gopi, Bruno S Lopes, Balaji Veeraraghavan
PURPOSE: This study aimed to characterize A. baumannii strains isolated from patients in an intensive care unit (ICU) setting. Molecular techniques were used to study clonal relatedness and determine a fast, efficient and cost-effective way of detecting persistent clones. METHODOLOGY: A. baumannii (n=17) were obtained in June and November 2015 from a single ICU setting in South India. DNA typing methods such as multilocus sequence typing (MLST), single-locus sequence-based typing (SBT) and DNA fingerprinting PCRs (M13, DAF4 and ERIC2) were employed to understand the association of clones...
July 17, 2018: Journal of Medical Microbiology
Zhong Chen, Hang Cheng, Weiguang Pan, Jinxin Zheng, Duoyun Li, Fojun Lin, Zhijian Yu, Qiwen Deng
PURPOSE: In this study, we aimed to investigate the genomic characteristics and evolution of pathogenicity islands of an enteropathogenic Escherichia coli (EPEC) strain, and to obtain a transcriptional profile of EPEC under different concentrations of ciprofloxacin using microarray analysis. METHODOLOGY: The complete EPEC Deng genome was sequenced and compared to genomes of 12 previously sequenced E. coli strains. A 180 min time course experiment was performed in which the effect of ciprofloxacin on EPEC Deng growth was evaluated...
July 10, 2018: Journal of Medical Microbiology
Charlotte van der Veer, Robin van Houdt, Alje van Dam, Henry de Vries, Sylvia Bruisten
PURPOSE: Bacterial vaginosis (BV) is a common clinical condition characterized by odorous vaginal discharge, vaginal itching and/or burning. BV can occur when vaginal lactobacilli are depleted and replaced by diverse anaerobic bacteria. We evaluated a commercial multiplex PCR (ATRiDA) for the diagnosis of BV. METHODS: Cervicovaginal samples were included from women reporting urogenital symptoms and from women notified for sexually transmitted infections (STI) - who were not (necessarily) symptomatic...
July 9, 2018: Journal of Medical Microbiology
Nicola Serra, Paola Di Carlo, Gaspare Gulotta, Francesco d' Arpa, Anna Giammanco, Claudia Colomba, Giuseppina Melfa, Teresa Fasciana, Consolato Sergi
PURPOSE: Bile is a hepatobiliary lipid-rich sterile solution, and its colonization by microorganisms defines the condition of bactibilia. In this study, we aimed to assess the bile microbiological flora and its potential link with comorbidity in women. METHODOLOGY: We performed a microbiologic investigation on 53 female patients with biliopancreatic diseases who granted consent, and we analysed the data using a MATLAB platform. RESULTS: We found that the most frequent disease associated with bactibilia was pancreas head carcinoma (PHC) (P=0...
July 5, 2018: Journal of Medical Microbiology
Hiroki Uchida, Tatsuya Tada, Yoshie Sugahara, Akira Kato, Isao Miyairi, Teruo Kirikae
The mcr-1 gene encodes a phosphoethanolamine transferase, which confers resistance to colistin by transferring phosphoethanolamine to lipid A. This study describes the emergence of a colistin- and carbapenem-resistant clinical isolate of Escherichia coli harbouring mcr-1 and blaNDM-5 genes, located on 90 and 150 Kb plasmids, respectively. The isolate belonged to ST132. This is the first report of a clinical isolate in Japan co-harbouring mcr-1 and blaNDM-5.
July 4, 2018: Journal of Medical Microbiology
Huseyin Sahinturk, Bulent Baran, Gurhan Sisman, Reskan Altun
We investigated the incidence of and risk factors for liver involvement (LI) in patients with brucellosis in Turkey. Diagnosis was established by blood cultures (BCs) or compatible clinical presentation accompanied by serological evidence. Patients received either a combination of doxycycline plus rifampin for 6 weeks or doxycycline for 6 weeks plus streptomycin intramuscularly for the first 2 weeks. Liver function tests, including those for alanine (ALT) and aspartate aminotransferase (AST) levels, were monitored before and at the end of treatment...
July 4, 2018: Journal of Medical Microbiology
Cédric Rafat, Jonathan Messika, Guilène Barnaud, Nicolas Dufour, Fatma Magdoud, Typhaine Billard-Pomarès, Stéphane Gaudry, Didier Dreyfuss, Catherine Branger, Dominique Decré, Jean-Damien Ricard
PURPOSE: Hypervirulent Klebsiella pneumoniae (hvKp) has emerged as a leading cause of severe community-acquired pneumonia, liver abscess and disseminated infection in the Far East. Data regarding the incidence, clinical features and microbiological characteristics related to hvKp infections in the Western world are scarce. METHODOLOGY: The incidence, clinical features and microbiological characteristics of hvKp infections were investigated through a 5-year survey conducted in a single French intensive care unit...
July 4, 2018: Journal of Medical Microbiology
Hossein Hosseini-Nave, Maryam Samareh Salavati, Mahla Lashkari, Mehrnaze Alsadat Ravari, Zahra Hashemizadeh, Farhad Seyedi Marghaki, Fahimeh Mohseni
PURPOSE: Entero-aggregative Escherichia coli (EAEC) is one of the main causes of diarrhoea worldwide. Several virulence factors have been identified in EAEC. This study was conducted to investigate the distribution of virulence factor genes in EAEC strains isolated in Iran from children with diarrhoea, as well as the genetic similarity of these isolates. METHODOLOGY: A total of 37 EAEC isolates were tested for the presence of 11 virulence genes by PCR, and the genetic relatedness of these strains was further determined by multilocus variable-number tandem-repeat analysis (MLVA)...
July 3, 2018: Journal of Medical Microbiology
Rajat Dhakal, Qinning Wang, Ruiting Lan, Peter Howard, Vitali Sintchenko
PURPOSE: Both Shigella and enteroinvasive Escherichia coli (EIEC) can cause enterocolitis, but they have a distinct epidemiology and public health relevance. Current culture-independent testing (CIT) methods to identify Shigella in faecal samples rely on the ipaH gene as the target, which is also found in EIEC genomes. The aim of this study was to design an assay that can identify EIEC in cultures from CIT ipaH-positive samples. METHODOLOGY: Shigella and EIEC genomes were screened to find unique regions present in EIEC genomes using a comparative genomics approach and differentiating genetic loci that are suitable PCR targets were identified...
July 3, 2018: Journal of Medical Microbiology
Helena Žemličková, Lucia Mališová, Petra Španělová, Vladislav Jakubů, Jana Kozáková, Martin Musílek, Matej Medvecký, Costas C Papagiannitsis
No abstract text is available yet for this article.
June 29, 2018: Journal of Medical Microbiology
Lauren M Terry, Clare R Barker, Martin R Day, David R Greig, Timothy J Dallman, Claire Jenkins
PURPOSE: Antimicrobial resistance (AMR) profiles of 754 strains of Shigella dysenteriae isolated between 2004 and 2017 from UK travellers reporting symptoms of gastrointestinal (GI) disease were reviewed to look for evidence of emerging AMR associated with travellers' diarrhoea. METHODOLOGY: A travel history was provided for 72.7 % (548/754) of cases, of which 90.9 % (498/548) reported travel outside the UK within 7 days of onset of symptoms, and 9.1 % (50/498) reported no travel in that time frame...
June 29, 2018: Journal of Medical Microbiology
A J Hughes, C A Knoten, A R Morris, A R Hauser
PURPOSE: Pseudomonas aeruginosa expresses a type III secretion system (T3SS) that activates the host inflammasome-mediated immune response. We examined the role of inflammasome activation in severe infection outcomes. METHODS: We infected C57BL/6 (B6) mice lacking inflammasome components ASC or caspase-1/11 with a highly virulent strain of P. aeruginosa, PSE9, using a mouse model of pneumonia. We evaluated inflammasome activation in vitro by infecting bone marrow-derived macrophages (BMDMs) with PSE9 and measuring cell death and release of inflammasome-dependent cytokines IL-18 and IL-1β...
June 29, 2018: Journal of Medical Microbiology
Mengkai Qiao, Wei Yong, Xuan Wang, Wei Li, Zhong Zhang, Min He, Limin Shi, Yaqian Wang, Guoxiang Xie, Jie Ding
PURPOSE: Enteroviruses (EV) 71 and coxsackievirus A (CVA) 16 are the most prevalent EV serotypes responsible for hand, foot and mouth disease (HFMD). Nevertheless, CVA6 was found to be the leading cause of HFMD in the Nanjing area, of China in 2013. This study aims to provide insights into the occurrence of the emergent recombinant CVA6 through examination of the evolutionary history and the involved recombination events. METHODOLOGY: The viral protein1 (VP1) and non-structural (NS) 2C and 3D of 28 Nanjing CVA6 strains were aligned, among which the full-length sequences of eight strains were further characterized...
June 27, 2018: Journal of Medical Microbiology
E Lopes, S Piçarra, P L Almeida, H de Lencastre, M Aires-de-Sousa
Multidrug-resistant bacteria pose a major threat to effective antibiotics and alternatives to fight multidrug-resistant pathogens are needed. We synthetized molybdenum oxide (MoO3) nanoparticles (NP) and determined their antibacterial activity against 39 isolates: (i) eight Staphylococcus aureus, including representatives of methicillin-resistant S. aureus epidemic clones; (ii) six enterococci, including vancomycin-resistant isolates; and (iii) 25 Gram-negative isolates (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Enterobacter cloacae), including extended spectrum beta-lactamases and carbapenemases producers...
June 25, 2018: Journal of Medical Microbiology
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