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Journal of Medical Microbiology

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https://www.readbyqxmd.com/read/28150580/biocompatible-biodegradable-polymeric-antibacterial-nanoparticles-for-enhancing-the-effects-of-the-third-generation-cephalosporin-against-resistant-bacteria
#1
Sana Mushtaq, Jawad Akbar Khan, Faiz Rabbani, Usman Latif, Muhammad Arfan, Muhammad Arfat Yameen
PURPOSE: The present study attempted to enhance the antibacterial activity of ceftriaxone with biodegradable polymer against Gram-positive (MRSA) and -negative (E. coli) bacteria. METHODOLOGY: MRSA and E. coli were collected and identified by biochemical and molecular tests. Blank and ceftriaxone loaded CNPs were prepared by ionic gelation method. The in-vitro antibiotic susceptibility studies were performed by disc diffusion, agar well plate method, E-test and time kill assay...
February 1, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28150578/resistotyping-phenotyping-and-genotyping-of-new-delhi-metallo-%C3%AE-lactamase-ndm-among-gram-negative-bacilli-from-iranian-patients
#2
Dariush Shokri, Mohammad Rabbani Khorasgani, Seyed Masih Fatemi, Abbas Soleimani-Delfan
PURPOSE: The purpose of this study was investigation of New Delhi metallo-β-lactamase (NDM) production among gram negative bacilli. METHODOLOGY: Antibiogram-Resistotyping and detection of NDM in clinical isolates of Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa and comparative evaluation of the diagnostic performance of three phenotypic methods for NDM detection considering PCR as gold standard were performed. Minimum Inhibitory Concentration (MIC) of antibiotics against NDM positive strains by E-test and clonal relationship analysis using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) in these strains were determined...
February 1, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28141509/development-validation-and-testing-costs-of-an-in-house-real-time-polymerase-chain-reaction-assay-for-the-detection-of-chlamydia-trachomatis
#3
Camila Gurgel Dos Santos, Meritxell Sabidó, André Luiz Leturiondo, Cynthia de Oliveira Ferreira, Thielle Pereira da Cruz, Adele Schwartz Benzaken
To improve screening of Chlamydia trachomatis (CT) in Brazil, an accurate and affordable method is needed. The objective of this study was to develop and assess the performance and costs of a new in-house real time polymerase chain reaction (qPCR) assay for the diagnosis of CT infection. Asymptomatic women aged 14-25 years who attended primary health services in Manaus, Brazil, were screened for CT using the Digene® Hybrid Capture II (HCII CT-ID) CT-ID DNA-test. A subset of cervical specimens were tested using an in-house qPCR and a commercial qPCR (Artus qPCR) as a reference test...
January 27, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28141498/first-genotype-identification-of-trichosporon-asahii-in-sfax-tunisia
#4
Hayet Sellami, Houaida Trabelsi, Sourour Neji, Imen Amouri, Fatma Cheikhrouhou, Fattouma Makni, Ali Ayadi
PURPOSE: The objectives of our study were species identification and genotyping of Trichosporon isolates collected at the Parasitology and Mycology Laboratory in Sfax, Tunisia. METHODOLOGY: Molecular identification was carried out by analyzing the IGS1 regions of the rDNA of 30 Trichosporon isolates. RESULTS: Trichosporon asahii was the most frequent species detected. Furthermore, four genotypes were identified in Tunisia: 1 (46.4%), 4 (35...
January 27, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28126051/loop-mediated-isothermal-amplification-lamp-assay-for-rapid-detection-of-streptococcus-agalactiae-group-b-streptococcus-gbs-in-vaginal-swabs-a-proof-of-concept-study
#5
James Patrick McKenna, Ciara Cox, Derek John Fairley, Rachael Burke, Michael D Shields, Alison Watt, Peter Valentine Coyle
PURPOSE: Neonatal infection with Streptococcus agalactiae (Group B streptococcus - GBS) is a life threatening condition which is preventable if colonised mothers are identified and given antibiotic prophylaxis during labour. Conventional culture is time consuming and unreliable, and many available non-culture diagnostics are too complex to implement routinely at point of care. Loop-mediated isothermal amplification (LAMP) is a method which enables the rapid and specific detection of target nucleic acid sequences in clinical material without requirements for extensive sample preparation...
January 24, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28126049/molecular-epidemiological-survey-of-treponema-pallidum-in-pregnant-women-in-the-zhabei-district-of-shanghai
#6
Yingying Lu, Qi Wu, Li Wang, Lingting Ji
:  Objectives: To evaluate the epidemiological characteristics of syphilis, such as incidence, vertical transmission, and genotypes, in pregnant women in the Zhabei District of Shanghai. In addition, the changes in genotypes and the efficiency of genotyping was evaluated. METHODS: We screened 3,022 pregnant women for syphilis in the Shanghai Zhabei Central Hospital; Whole blood , plasma, earlobe blood, and specimens from genital ulcers or skin/mucosal lesions were collected from syphilis positive patients...
January 24, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28126044/rv1458c-a-new-diagnostic-marker-for-identification-of-m-tuberculosis-complex-in-a-novel-duplex-pcr-assay
#7
Kamal Shrivastava, Kushal Garima, Anshika Narang, Kausik Bhattacharyya, Ekta Vishnoi, Roshan Kumar Singh, Anil Choudhary, Rajendra Prasad, Mridula Bose, Mandira Varma-Basil
We explored the efficiency of Rv1458c, a putative ABC drug transporter specific for the Mycobacterium tuberculosis complex (MTBC) as a diagnostic marker. A 190 bp region of Rv1458c and a 300bp region of hsp65 were targeted in a novel duplex PCR assay and compared with PCR restriction analysis (PRA) using restriction enzymes NruI and BamHI. Speciation of a subset of the isolates (n=50) was confirmed by sequencing. Clinical isolates (n=426) obtained from clinically suspected patients of pulmonary tuberculosis; and mycobacterial (n=13) and non-mycobacterial (n=8) reference strains were included in the study...
January 23, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28126043/high-yield-extraction-of-e-coli-rna-from-human-whole-blood
#8
Johannes Brennecke, Simone Kraut, Klara Zwadlo, Senthil Kumar Gandi, David Pritchard, Kate Templeton, Till Bachmann
Studies of bacterial transcriptomics during bloodstream infections are limited to-date because unbiased extraction of bacterial mRNA from whole blood in sufficient quantity and quality has proved challenging. We addressed the key aspect of this issue and investigated approaches for the lysis of the human blood cells and the stabilisation of bacterial RNA in human whole blood samples containing E. coli cells. We compared four lysis agents: Triton X-100, saponin, ammonium chloride and the commercial MolYsis lysis buffer CM for their ability to yield high amounts of undegraded RNA and investigated their impact on the bacterial gene expression...
January 23, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28113042/molecular-diagnosis-of-non-serotypeable-shigella-spp-problems-and-prospects
#9
Dhiviya Prabaa Muthuirulandi Sethuvel, Naveen Kumar Devanga Ragupathi, Shalini Anandan, Kamini Walia, Balaji Veeraraghavan
No abstract text is available yet for this article.
January 22, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28109201/genetic-analyses-of-the-fusion-protein-genes-in-human-parainfluenza-virus-types-1-and-3-among-patients-with-acute-respiratory-infections-in-eastern-japan-from-2011-to-2015
#10
Rika Tsutsui, Hiroyuki Tsukagoshi, Koo Nagasawa, Masaki Takahashi, Yuki Matsushima, Akihide Ryo, Makoto Kuroda, Hideki Takami, Hirokazu Kimura
PURPOSE: To genetically explorer the fusion protein gene (F) in human parainfluenza virus type 1 (HPIV1) and type 3 (HPIV3) strains, we analyzed them in the patients with acute respiratory infections (ARI) in Eastern Japan from 2011 to 2015. METHODOLOGY: We constructed phylogenetic trees based on the HPIV and HPIV3 F gene using the maximum likelihood method and conducted p-distance, and selective pressure analyses. We also predicted the linear epitopes of the protein in the prototype strains...
January 20, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28100314/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-rapid-identification-of-fungal-rhinosinusitis-pathogens
#11
Xinxin Lu, Yanfei Huang, Mingxin Zhang, Min Zhu, Mei Wang, Yufeng Sun, Haitong Gu, Jingjing Cao, Xue Li, Shaoya Zhang, Jinglin Wang
BACKGROUND: filamentous fungi are the most important pathogens causing fungal rhinosinusitis (FRS). Current laboratory diagnosis of FRS pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time-consuming and expertise-dependent. Although matrix -assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been employed to identify various fungi, its performance for the identification of FRS fungi is less clear...
January 17, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28100300/molecular-analysis-and-species-diversity-of-nocardia-in-hospital-environment-of-a-developing-country-a-potential-health-hazard
#12
Hossein Ali Rahdar, Davood Azadi, Hasan Shojaei, Abbas Daei Naser
BACKGROUND: Despite hundreds of reports on the isolation of Nocardia from clinical samples, the presence and diversity of Nocardia species capable of survival in a harsh and adverse condition such as hospital environment have not been comprehensively studied. The aim of this study was to assess the Nocardia species diversity in the hospital environment to provide a better insight on their potential threat as a reservoir for the development of nosocomial infections. MATERIALS AND METHODS: A total of 90 hospital water, dust and soil samples collected from 30 hospitals were analyzed for the presence of Nocardia using standard protocols for isolation and characterization of the isolates...
January 17, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28100311/the-evaluation-of-three-different-bottles-in-bactec-9240-automated-blood-culture-system-and-direct-identification-of-candida-species-to-shorten-the-turnaround-time-of-blood-culture
#13
Egemen Gokbolat, Yasemin Oz, Selma Metintas
Candida spp. are the most common causes of fungemia. Rapid and accurate diagnostic methods are very important for appropriate management of candidemia. At present, blood culture is the essential diagnostic test despite it has a long detection time and low sensitivity rate. We aimed to investigate the ways to shorten the turnaround time from blood culture collection to final identification in candidemia. Sixty clinical bloodstream isolates of Candida were included and the Plus Aerobic/F, Peds Plus/F and Mycosis IC/F (MICF) bottles were used with BACTEC 9240 blood culture instrument...
January 16, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28086079/a-large-prolonged-outbreak-of-hepatitis-a-associated-with-consumption-of-frozen-berries-italy-2013-14
#14
Gaia Scavia, Valeria Alfonsi, Stefania Taffon, Martina Escher, Roberto Bruni, Dario De Medici, Simona Di Pasquale, Sarah Guizzardi, Benedetta Cappelletti, Stefania Iannazzo, Nadia Marina Losio, Enrico Pavoni, Lucia Decastelli, Anna Rita Ciccaglione, Michele Equestre, Maria Elena Tosti, Caterina Rizzo
In 2013/2014 Italy experienced one of the largest community-wide prolonged outbreak of Hepatitis A virus (HAV) through over the country. Enhanced human epidemiological and microbiological surveillance together with microbiological monitoring of HAV in food and trace-back investigation were conducted in order to give a comprehensive description of the outbreak investigation carried out in Italy by a multidisciplinary National Task Force in collaboration with regional and local public health authorities. Moreover, control strategies of foodborne HAV infection in both the human and food sectors are described...
January 13, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28086078/role-and-mechanism-of-the-hsp70-molecular-chaperone-machines-in-bacterial-pathogens
#15
Ciamak- Ghazaei
Heat shock proteins (HSP) are highly conserved, stress-inducible, ubiquitous proteins that maintain homeostasis in both eukaryotes and prokaryotes. Hsp70 proteins belong to the HSP family and enhance bacterial survival in hostile environments. Hsp70, known as DnaK in prokaryotes, supports numerous processes such as the assembly and disassembly of protein complexes, the refolding of misfolded and clustered proteins, membrane translocation, and the regulation of regulatory proteins. The chaperone-based activity of Hsp70 depends on dynamic interactions between its two domains known as the ATPase domain and the substrate binding domain...
January 12, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28086073/evolutionary-and-genetic-analysis-of-human-bocavirus-genotype-1-strains-reveals-an-evidence-of-intragenomic-recombination
#16
Ahmed S Abdel-Moneim, Mahmoud M Kamel, Naglaa M Hassan
Human bocavirus (HBoV) is divided into 4 genotypes: 1 to 4 with the HBoV-1 is the most commonly prevalent genotype. The current study intended to genetically analyze the full length genome of the HBoV-1 of recently detected Egyptian strains. Seven overlapping sets of primers were developed to amplify a nearly complete HBoV-1 genome from the clinical samples. The primer sets were tested on three recently identified Egyptian HBoV-1 strains with viral loads ≥105/ml. Sequencing was conducted using the same sets of primers...
January 11, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28086068/improved-effect-of-amikacin-loaded-poly-d-l-lactide-co-glycolide-plga-nanoparticles-against-planktonic-and-biofilm-cells-of-pseudomonas-aeruginosa
#17
Parastoo Sabaeifard, Ahya Abdi-Ali, Carlos Gamazo, Juan M Irache, Mohammad Reza Soudi
Amikacin is one of the most effective antibiotics against Pseudomonas aeruginosa infections but, according to its high toxicity, the use of this antibiotic has been clinically limited. In the present study, amikacin was successfully loaded into a new formulation of nanoparticles based on PLGA 50:50. Amikacin-loaded nanoparticles showed desirable antibacterial and antibiofilm characteristics. Flow cytometric studies showed that these nanoparticles were able to interact with Planktonic and biofilm bacterial cells...
January 11, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28068218/comparison-of-self-collected-meatal-swabs-with-urine-specimens-for-the-diagnosis-of-chlamydia-trachomatis-and-neisseria-gonorrhoeae-in-men
#18
Lisa Berry, Belinda Stanley
Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) are common bacterial causes of sexually transmitted infection. Self-taken meatal swabs are a possible alternative to urine samples in testing for infection; however the data surrounding their use are limited. We carried out a prospective service review in a large sexual health clinic comparing urine samples and self-taken meatal swabs in men presenting for sexual transmissible infection screening for CT and GC with the BD Viper XTR system. We found an overall prevalence of CT infections was 10...
January 9, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28068217/manual-curation-and-reannotation-of-the-genomes-of-clostridium-difficile-630%C3%AE-erm-and-clostridium-difficile-630
#19
Henning Dannheim, Thomas Riedel, Meina Neumann-Schaal, Boyke Bunk, Isabel Schober, Cathrin Spröer, Cynthia Maria Chibani, Sabine Gronow, Heiko Liesegang, Jörg Overmann, Dietmar Schomburg
We resequenced the genome of Clostridium difficile 630Δerm (DSM 28645), a model strain commonly used for the generation of insertion mutants. The genome sequence was obtained by a combination of single-molecule real-time (SMRT) and Illumina sequencing technology. Detailed manual curation and comparison to the previously published genomic sequence revealed sequence differences including inverted regions and the presence of plasmid pCD630. Manual curation of our previously deposited genome sequence of the parental strain 630 (DSM 27543) led to an improved genome sequence...
January 9, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28068213/utilization-of-size-polymorphism-in-its1-and-its2-regions-for-identification-of-pathogenic-yeast-species
#20
Hossein Khodadadi, Ladan Karimi, Niloufar Jalali-Zand, Hassan Adin, Hossein Mirhendi
Despite the existence of a variety of available yeast identification strategies, easier and more cost-effective methods are required for routine use in clinical laboratories. The internal transcribed spacer (ITS) regions of fungal rRNA genes exhibit variable sizes depending on the yeast species. In the present study, fragment size polymorphism (FSP) analysis of the ITS1 and ITS2 regions for identification of the clinically most important yeast species was assessed. The ITS1 and ITS2 regions of 190 strains, including isolates of 31 standard strains and 159 clinical isolates, were separately PCR-amplified with two primer sets: ITS1-ITS2 and ITS3-ITS4...
January 9, 2017: Journal of Medical Microbiology
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