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Biochimica et Biophysica Acta

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https://www.readbyqxmd.com/read/28232091/purification-characterization-and-cytotoxicity-assessment-of-ageritin-the-first-ribotoxin-from-the-basidiomycete-mushroom-agrocybe-aegerita
#1
Nicola Landi, Severina Pacifico, Sara Ragucci, Rosario Iglesias, Simona Picolella, Adolfo Amici, Antonella M A Di Giuseppe, Antimo Di Maro
BACKGROUND: Several species belonging to Ascomycota phylum produce extracellular ribonucleases, known as ribotoxins, which exhibit RNase activity through the cleavage of a single phosphodiester bond, located at the universally conserved sarcin/ricin loop of the large rRNA leading inhibition of protein biosynthesis. Clarifying the structure-function relationship in ribotoxins is interesting for their use in human tumour therapy and in construction of pests resistant transgenic plants. RESULTS: The ribotoxin Ageritin has been isolated for the first time from Basidiomycetes class...
February 19, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28232026/the-crystal-structures-of-native-hydroquinone-1-2-dioxygenase-from-sphingomonas-sp-ttnp3-and-of-substrate-and-inhibitor-complexes
#2
Marta Ferraroni, Stefano Da Vela, Boris A Kolvenbach, Philippe F X Corvini, Andrea Scozzafava
The crystal structure of hydroquinone 1,2-dioxygenase, a Fe(II) ring cleaving dioxygenase from Sphingomonas sp. strain TTNP3, which oxidizes a wide range of hydroquinones to the corresponding 4-hydroxymuconic semialdehydes, has been solved by Molecular Replacement, using the coordinates of PnpCD from Pseudomonas sp. strain WBC-3. The enzyme is a heterotetramer, constituted of two subunits α and two β of 19 and 38kDa, respectively. Both the two subunits fold as a cupin, but that of the small α subunit lacks a competent metal binding pocket...
February 19, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28219769/epitranscriptomic-regulation-of-viral-replication
#3
REVIEW
Camila Pereira-Montecinos, Fernando Valiente-Echeverría, Ricardo Soto-Rifo
RNA plays central roles in biology and novel functions and regulation mechanisms are constantly emerging. To accomplish some of their functions within the cell, RNA molecules undergo hundreds of chemical modifications from which N6-methyladenosine (m(6)A), inosine (I), pseudouridine (ψ) and 5-methylcytosine (5mC) have been described in eukaryotic mRNA. Interestingly, the m(6)A modification was shown to be reversible, adding novel layers of regulation of gene expression through what is now recognized as epitranscriptomics...
February 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28219768/copper-therapy-reduces-intravascular-hemolysis-and-derepresses-ferroportin-in-mice-with-mosaic-mutation-atp7a-mo-ms-an-implication-for-copper-mediated-regulation-of-the-slc40a1-gene-expression
#4
Małgorzata Lenartowicz, Rafał R Starzyński, Aneta Jończy, Robert Staroń, Justyna Antoniuk, Wojciech Krzeptowski, Paweł Grzmil, Aleksandra Bednarz, Olga Pierzchała, Mateusz Ogórek, Zenon Rajfur, Zbigniew Baster, Paweł Lipiński
Mosaic mutant mice displaying functional dysfunction of Atp7a copper transporter (the Menkes ATPase) are an established animal model of Menkes disease and constitute a convenient tool for investigating connections between copper and iron metabolisms. This model allows to explore changes in iron metabolism in suckling mutant mice suffering from systemic copper deficiency as well as in young and adult ones undergone copper therapy, which reduces lethal effect of the Atp7a gene mutation. Our recent study demonstrated that 14-day-old mosaic mutant males display blood cell abnormalities associated with intravascular hemolysis, and show disturbances in the functioning of the hepcidin-ferroportin regulatory axis, which controls systemic iron homeostasis...
February 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28219767/the-biochemical-alterations-underlying-post-burn-hypermetabolism
#5
REVIEW
Christopher Auger, Osai Samadi, Marc G Jeschke
A severe burn can trigger a hypermetabolic state which lasts for years following the injury, to the detriment of the patient. The drastic increase in metabolic demands during this phase renders it difficult to meet the body's nutritional requirements, thus increasing muscle, bone and adipose catabolism and predisposing the patient to a host of disorders such as multi-organ dysfunction and sepsis, or even death. Despite advances in burn care over the last 50 years, due to the multifactorial nature of the hypermetabolic phenomenon it is difficult if not impossible to precisely identify and pharmacologically modulate the biological mediators contributing to this substantial metabolic derangement...
February 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28219766/mitochondrial-and-endoplasmic-reticulum-dysfunction-and-related-defense-mechanisms-in-critical-illness-induced-multiple-organ-failure
#6
REVIEW
Steven E Thiessen, Greet Van den Berghe, Ilse Vanhorebeek
Patients with critical illness-induced multiple organ failure suffer from a very high morbidity and mortality, despite major progress in intensive care. The pathogenesis of this condition is complex and incompletely understood. Inadequate tissue perfusion and an overwhelming inflammatory response with pronounced cellular damage have been suggested to play an important role, but interventions targeting these disturbances largely failed to improve patient outcome. Hence, new therapeutic perspectives are urgently needed...
February 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28219722/regulation-and-control-of-nitric-oxide-no-in-macrophages-protecting-the-professional-killer-cell-from-its-own-cytotoxic-arsenal-via-mrp1-and-gstp1
#7
REVIEW
Z Kovacevic, S Sahni, H Lok, M J Davies, D A Wink, D R Richardson
We recently demonstrated that a novel storage and transport mechanism for nitric oxide (NO) mediated by glutathione-S-transferase P1 (GSTP1) and multidrug resistance protein 1 (MRP1/ABCC1), protects M1-macrophage (M1-MØ) models from large quantities of endogenous NO. This system stores and transports NO as dinitrosyl-dithiol-iron complexes (DNICs) composed of iron, NO and glutathione (GSH). Hence, this gas with contrasting anti- and pro-tumor effects, which has been assumed to be a freely diffusible, is a tightly-regulated species in M1-MØs...
February 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28219721/magnetic-nanoparticles-a-multifunctional-vehicle-for-modern-theranostics
#8
REVIEW
M Angelakeris
Magnetic nanoparticles provide a unique multifunctional vehicle for modern theranostics since they can be remotely and non-invasively employed as imaging probes, carrier vectors and smart actuators. Additionally, special delivery schemes beyond the typical drug delivery such as heat or mechanical stress may be magnetically triggered to promote certain cellular pathways. To start with, we need magnetic nanoparticles with several well-defined and reproducible structural, physical, and chemical features, while bio-magnetic nanoparticle design imposes several additional constraints...
February 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28219720/lipidomics-in-research-on-yeast-membrane-lipid-homeostasis
#9
Anton I P M de Kroon
Mass spectrometry is increasingly used in research on membrane lipid homeostasis, both in analyses of the steady state lipidome at the level of molecular lipid species, and in pulse-chase approaches employing stable isotope-labeled lipid precursors addressing the dynamics of lipid metabolism. Here my experience with, and view on mass spectrometry-based lipid analysis is presented, with emphasis on aspects of quantification of membrane lipid composition of the yeast Saccharomyces cerevisiae. This article is part of a Special Issue entitled: BBALIP_Lipidomics Opinion Articles edited by Sepp Kohlwein...
February 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28219719/cysteine-deprivation-prevents-induction-of-peroxisome-proliferator-activated-receptor-gamma-2-and-adipose-differentiation-of-3t3-l1-cells
#10
Nadia Nabil Haj-Yasein, Ole Berg, Fredrik Jernerén, Helga Refsum, Hilde I Nebb, Knut Tomas Dalen
Plasma cysteine is strongly associated with body fat mass in human cohorts and diets low in cysteine prevents fat accumulation in mice. It is unclear if plasma cysteine affects fat development or if fat accumulation raises plasma cysteine. To determine if cysteine affects adipogenesis, we differentiated 3T3-L1 preadipocytes in medium with reduced cysteine. Cells incubated in media with 10-20μM cysteine exhibited reduced capacity to differentiate into triacylglycerol-storing mature adipocytes compared with cells incubated with 50μM cysteine...
February 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28212887/erratum-to-toxoplasma-gondii-immune-mapped-protein-1-is-anchored-to-the-inner-leaflet-of-the-plasma-membrane-and-adopts-a-novel-protein-fold-biochim-biophys-acta-protein-proteomics-1865-2-2017-208-219
#11
Yonggen Jia, Stefi Benjamin, Qun Liu, Yingqi Xu, Sunil Kumar Dogga, Jing Liu, Stephen Matthews, Dominique Soldati-Favre
No abstract text is available yet for this article.
February 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28216426/no-control-of-mitochondrial-function-in-normal-and-transformed-cells
#12
Celia H Tengan, Carlos T Moraes
Nitric oxide (NO) is a signaling molecule with multiple facets and involved in numerous pathological process, including cancer. Among the different pathways where NO has a functionally relevant participation, is the control of mitochondrial respiration and biogenesis. NO is able to inhibit the electron transport chain, mainly at Complex IV, regulating oxygen consumption and ATP generation, but at the same time, can also induce increase in reactive oxygen and nitrogen species. The presence of reactive species can induce oxidative damage or participate in redox signaling...
February 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28216340/cellular-vacuolization-caused-by-overexpression-of-the-pikfyve-binding-deficient-vac14-l156r-is-rescued-by-starvation-and-inhibition-of-vacuolar-atpase
#13
Ulf Schulze, Beate Vollenbröker, Alexander Kühnl, Daniel Granado, Samet Bayraktar, Ursula Rescher, Hermann Pavenstädt, Thomas Weide
Phosphoinositides (PI) and converting enzymes are crucial determinants of organelle identity and morphology. One important endolysosomal specific PI is PI(3,5)P2, generated by the PIKfyve kinase, which orchestrates in combination with Vac14 and Fig4. Dysfunction of this complex leads to large intracellular vacuoles in various cell types and is linked to neurological diseases. Here, we characterize the vacuolization phenotype caused by overexpression of the PIKfyve binding deficient mutant Vac14(L156R) in podocytes, which represent specialized cells of the kidney...
February 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28216225/molecular-characterization-of-myoglobin-from-sciurus-vulgaris-meridionalis-primary-structure-kinetics-and-spectroscopic-studies
#14
Antonella M A Di Giuseppe, Luigi Russo, Rosita Russo, Sara Ragucci, J Valentina Caso, Carla Isernia, Angela Chambery, Antimo Di Maro
Myoglobins (Mbs) are heme-proteins involved in dioxygen storage necessary for metabolic respiration. Mbs are intensely investigated as archetype to investigate structure/function relationship in globular proteins. In this work, the myoglobin from S. vulgaris meridionalis has been for the first time isolated and purified with a high yield and homogeneity. The primary structure characterization has been performed by applying a strategy based on high resolution tandem mass spectrometry. Proximal (position 93, α-helix F8) and distal histidinyl residues (position 64, α-helix E7) as well as most of the amino acid residues (i...
February 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28216224/targeted-proteomics-driven-verification-of-biomarker-candidates-associated-with-breast-cancer-aggressiveness
#15
Iva Procházková, Juraj Lenčo, Alena Fučíková, Jiří Dresler, Lenka Čápková, Roman Hrstka, Rudolf Nenutil, Pavel Bouchal
Breast cancer is the most common and molecularly relatively well characterized malignant disease in women, however, its progression to metastatic cancer remains lethal for 78% of patients 5years after diagnosis. Novel markers could identify the high risk patients and their verification using quantitative methods is essential to overcome genetic, inter-tumor and intra-tumor variability and translate novel findings into cancer diagnosis and treatment. We recently identified 13 proteins associated with estrogen receptor, tumor grade and lymph node status, the key factors of breast cancer aggressiveness, using untargeted proteomics...
February 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28216054/lipidomics-en-route-to-accurate-quantitation
#16
REVIEW
Sin Man Lam, He Tian, Guanghou Shui
Accurate quantitation is prerequisite for the sustainable development of lipidomics via enabling its applications in various biological and biomedical settings. In this review, the technical considerations and limitations of existent lipidomics technologies, particularly in terms of accurate quantitation; as well as the potential sources of errors along a typical lipidomic workflow that could ultimately give rise to quantitative inaccuracies will be addressed. Furthermore, the pressing need to exercise stricter definitions of terms and protocol standardization pertaining to quantitative lipidomics will be critically discussed, as quantitative accuracy may substantially impact upon the persevering development of lipidomics in the long run...
February 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28216046/inactivation-of-iron-sulfur-cluster-biogenesis-regulator-sufr-in-synechocystis-sp-pcc-6803-induces-unique-iron-dependent-protein-level-responses
#17
Linda Vuorijoki, Arjun Tiwari, Pauli Kallio, Eva-Mari Aro
BACKGROUND: Iron-sulfur (Fe-S) clusters are protein-bound cofactors associated with cellular electron transport and redox sensing, with multiple specific functions in oxygen-evolving photosynthetic cyanobacteria. The aim here was to elucidate protein-level effects of the transcriptional repressor SufR involved in the regulation of Fe-S cluster biogenesis in the cyanobacterium Synechocystis sp. PCC 6803. METHODS: The approach was to quantitate 94 pre-selected target proteins associated with various metabolic functions using SRM in Synechocystis...
February 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28216027/corrigendum-to-metabolomic-based-biomarker-discovery-for-non-invasive-lung-cancer-screening-a-case-study-biochimica-et-biophysica-acta-bba-general-subjects-vol-1860-2016-pp-2682-2687
#18
Keiron O'Shea, Simon J S Cameron, Keir E Lewis, Chuan Lu, Luis A J Mur
No abstract text is available yet for this article.
February 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28215579/mitochondrial-dna-maintenance-defects
#19
REVIEW
Ayman W El-Hattab, William J Craigen, Fernando Scaglia
The maintenance of mitochondrial DNA (mtDNA) depends on a number of nuclear gene-encoded proteins including a battery of enzymes forming the replisome needed to synthesize mtDNA. These enzymes need to be in balanced quantities to function properly that is in part achieved by exchanging intramitochondrial contents through mitochondrial fusion. In addition, mtDNA synthesis requires a balanced supply of nucleotides that is achieved by nucleotide recycling inside the mitochondria and import from the cytosol. Mitochondrial DNA maintenance defects (MDMDs) are a group of diseases caused by pathogenic variants in the nuclear genes involved in mtDNA maintenance resulting in impaired mtDNA synthesis leading to quantitative (mtDNA depletion) and qualitative (multiple mtDNA deletions) defects in mtDNA...
February 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28215578/mfn2-protects-dopaminergic-neurons-exposed-to-paraquat-both-in-vitro-and-in-vivo-implications-for-idiopathic-parkinson-s-disease
#20
Fanpeng Zhao, Wenzhang Wang, Chunyu Wang, Sandra L Siedlak, Hisashi Fujioka, Beisha Tang, Xiongwei Zhu
Mitochondrial dynamics and quality control plays a critical role in the maintenance of mitochondrial homeostasis and function. Pathogenic mutations of many genes associated with familial Parkinson's disease (PD) caused abnormal mitochondrial dynamics, suggesting a likely involvement of disturbed mitochondrial fission/fusion in the pathogenesis of PD. In this study, we focused on the potential role of mitochondrial fission/fusion in idiopathic PD patients and in neuronal cells and animals exposed to paraquat (PQ), a commonly used herbicide and PD-related neurotoxin, as models for idiopathic PD...
February 16, 2017: Biochimica et Biophysica Acta
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