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Tissue & Cell

Dalia Ibrahim
The glycoconjugates pattern of acidic secretions and distribution of chemosensory cells (SCCs) in the respiratory mucosa of dromedary camels were analyzed so as to identify their functional role. Secretions of the goblet cells and mucous glandular cells were analyzed to evaluate the variety of sugar chains, focusing on the acidic glycoconjugates. Using lectin histochemistry, WGA, STL, DBA, SBA, VVA and RCA-120 intensely bound to the goblet cells. PNA and ECL labeled the goblet cells with moderate intensity...
April 2018: Tissue & Cell
Fei Peng, Xianxian Chen, Ting Meng, En Li, Yongkang Zhou, Shengzhou Zhang
The Chinese alligator Alligator sinensis is an endangered freshwater crocodilian species endemic to China. Hematology and serum biochemistry reference range are useful in the assessment and management of animal health condition. In this study, a total of 74 Chinese Alligators (30 males and 44 females) were examined to establish reference range values of hematology and serum biochemistry parameters during the active and hibernating periods. We measured and analyzed 9 hematology and 21 serum biochemistry parameters including 4 serum electrolyte parameters, and described the morphology of different types of blood cells...
April 2018: Tissue & Cell
V Gross, R Bährle, G Mayer
The taxon Tardigrada, commonly called "water bears", consists of microscopic, eight-legged invertebrates that are well known for their ability to tolerate extreme environmental conditions. Their miniscule body size means that tardigrades possess a small total number of cells, the number and arrangement of which may be highly conserved in some organs. Although mitoses have been observed in several organs, the rate and pattern of cell divisions in adult tardigrades has never been characterized. In this study, we incubated live tardigrades over a period of several days with a thymidine analog in order to visualize all cells that had divided during this time...
April 2018: Tissue & Cell
Hasan Huseyin Sahin, Alev Cumbul, Unal Uslu, Zeynep Yilmaz, Feriha Ercan, Inci Alican
This study evaluated ulceroprotective and antioxidant effect of 1,25 dihydroxyvitamin D3 against gastric damage in rats. Rats were treated intraperitoneally with either 1,25 dihydroxyvitamin D3 (0.25 μg/kg) or saline for 14 days. On day-15, the non-selective cyclooxygenase inhibitor indomethacin (10 mg/kg; subcutaneously), the inhibitor of sulfhydryl groups N-ethylmaleimide (10 mg/kg; intraperitoneally) or ATP-sensitive K+ channel blocker glibenclamide (10 mg/kg; orally) was given prior to 1,25 dihydroxyvitamin D3...
April 2018: Tissue & Cell
Bice Avallone, Monica Tizzano, Raimondo Cerciello, Maria Buglione, Domenico Fulgione
The epidermis of Tarentola mauritanica in the skin regions of back, flank and belly has been described using light and electron microscopy. This animal model was useful to give an insight of the functional pattern involved in pigmentation, cryptism and photosensitivity. Skin from back and flanks, in electron microscopy, shows a high concentration of chromatophores, among those melanophores, xanthophores and iridophores have been reported. Interestingly, in the flank-back transition region electron microscopy reveals the presence of nerve endings...
April 2018: Tissue & Cell
Marcelo Silva Barcellos, Glenda Dias, José Lino-Neto
The corn leafhopper, Dalbulus maidis, which is one of the most important vectors of maize diseases, had the morphology of its spermatozoa described more than 40 years ago. However, the description is imprecise, requiring certain rectifications. Hence, we re-describe the sperm morphology of this species using light microscopy and transmission electron microscopy. Their spermatozoa measured from 118.1 to 128.5 μm in length, this value being about three times smaller than what was previously described. The head region is formed by a small acrosome and the nucleus...
April 2018: Tissue & Cell
Dharmendra Kumar, Papori Sharma, Kennady Vijayalakshmy, Naresh L Selokar, Pradeep Kumar, Rasika Rajendran, P S Yadav
The objective of this study was to optimise the electroporation conditions for efficient integration of Venus construct in buffalo fetal fibroblasts using Sleeping Beauty (SB) based transposition and to produce Venus expressing transgenic cloned embryos through handmade cloning (HMC) approach. Primary culture of buffalo fetal fibroblast cells was established and subsequently cultured cells were co-transfected with Venus and helper plasmid at different combinations of electroporation condition. In different combinations of voltage, time and plasmid dose, we observed that 300 V, single pulse for 10 ms in 2 mm cuvette and 1...
April 2018: Tissue & Cell
Haiying Wang, Danping Wang, Chunsun Li, Xingsong Zhang, Xiaolin Zhou, Jianan Huang
Karyopherin β1 (Kpnβ1), also known as importin-β, is part of the karyopherin superfamily of nuclear transport proteins. Kpnβ1 is an oncogene that is overexpressed in various human cancers. Recent studies have showed that Kpnβ1 is one of the leading causes of cancer-related deaths in the world. However, the role of Kpnβ1 in non-small cell lung cancer (NSCLC) remains uncertain. In this study, we used western blotting to show that Kpnβ1 expression is higher in lung-cancer tissues and cells, and immunohistochemistry analysis revealed that Kpnβ1 was significantly associated with the clinicopathological features of NSCLC...
April 2018: Tissue & Cell
Saeid Vakilian, Mohammad Norouzi, Mahsa Soufi-Zomorrod, Iman Shabani, Simzar Hosseinzadeh, Masoud Soleimani
Since ancient times, some herbal medicines have been extensively used for burn and wound treatments, showing preference to the common synthetic medications by virtue of having less side effects and faster healing rate. In this study, hybrid nanofibrous scaffolds of poly-l-lactic-acid (PLLA) and gelatin incorporated L. inermis were fabricated via electrospinning technique. Morphology and characteristics of the scaffolds were studied by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR), respectively...
April 2018: Tissue & Cell
J Martínez-Salvador, A Ruiz-Torner, A Blasco-Serra, F Martínez-Soriano, A A Valverde-Navarro
We studied the effect of alcohol on the pineal gland of 48 male Wistar rats. Animals were divided into control and experimental groups. The experimental group underwent a previous progressive alcoholisation period with ethanol diluted in water at a concentration of 40%. Animals were sacrificed at 3, 6, 9 and 12 months, and the ultrastructure, karyometric indices, and number of synaptic bodies in the pineal gland were analysed. The results showed progressive morphologic alterations in the ethanol-treated animals, which culminated in fatty degeneration of the pineal parenchyma after 6 months...
April 2018: Tissue & Cell
Sandeep Kumar Vishwakarma, Nusrath Fatima, Chandrakala Lakkireddy, Nagarapu Raju, Avinash Bardia, A Sandhya, Syed Ameer Basha Paspala, Vishnupriya Satti, Aleem Ahmed Khan
INTRODUCTION: Ethanol exposure to developing brain may alter the growth and differentiation of neurological cells resulting in unfavorable pathologies. Earlier studies have provided very limited mechanistic insights of cellular and molecular mechanisms which do not mimic with human situation due to varying cell types and poses potential challenges for investigation. Therefore, the present study was undertaken to evaluate the role of ABC transporters and heat shock proteins mediated response in human neural precursor cells (NPCs) and its lineages during proliferation and lineage differentiation against ethanol exposure...
April 2018: Tissue & Cell
Ying Li, Yan Zhang, Shounan Qi, Guanfang Su
Reliable disease models are essential for investigations on mechanisms and therapies. The Retinal organotypic culture can maintain the architecture and cellular connections within the tissue in vitro. The system is a refined retinal experiment platform. It narrows the gap between cell line studies and in vivo models and is flexible enough for sophisticated experimental procedures. It greatly reduces the consumption of time or resources. Retinas of many species in various development stages have been used for diverse explorations based on their morphologies and physical characteristics...
April 2018: Tissue & Cell
Letícia Ferreira Alves, Raquel Frenedoso da Silva, Valéria Helena Alves Cagnon
Prostate cancer is the most prevalent type of cancer in men around the world. Due to its high incidence, new therapies have been evaluated, including drugs capable of inhibiting the FGF/VEGF pathways, as Nintedanib. The aim herein was to evaluate the Nintedanib therapeutic effects on morphology and COX-2 and IL-17 levels in the prostate anterior lobe in different grades of the tumor progression in TRAMP mice. Animals were treated with Nintedanib at a dose of 10 mg/kg/day in initial and intermediate grades of tumor development...
February 2018: Tissue & Cell
Hao Zhang, Ping Wang, Yixue Xue, Libo Liu, Zhen Li, Yunhui Liu
In middle and old age, Alzheimer's disease (AD) is a progressive neurodegenerative disorder of brain. As an increasingly aging population, AD represents a huge burden for the patients' family and the country. However, current therapeutical strategies have shown limited effectiveness. Allicin, which is the main composition of garlic, was reported to prevent the learning and memory impairment of AD mouse model. As the mechanism is not clear, in this study, we used the APP (amyloid precursor protein)/PS1 (presenilin 1) double transgenic mice, which express human mutant APP and PS1, to determine the protective effect of allicin on neurons...
February 2018: Tissue & Cell
Katarzyna Palus, Michał Bulc, Marta Czajkowska, Bartosz Miciński, Jarosław Całka
The present study was designed to determine the distribution, morphology and co-localization of calbindin-D28k (CB) with other neuroactive substances in the coeliac-cranial mesenteric ganglion complex (CCMG) neurons supplying the prepyloric region of the porcine stomach. In all animals, a median laparotomy was performed and the fluorescent retrograde neuronal tracer Fast Blue was injected into the wall of the stomach prepyloric area. On the 28th day, all animals were euthanized and the CCMG complexes were then collected and processed for double-labelling immunofluorescence for CB and tyrosine hydroxylase (TH), galanin (GAL), somatostatin (SOM), leu 5-enkephalin (LENK), vasoactive intestinal peptide (VIP), substance P (SP) and cocaine- and amphetamine-regulated transcript peptide (CART), Immunohistochemistry revealed that 8...
February 2018: Tissue & Cell
G H B Andrade, V A Simão, B R Souza, L G A Chuffa, I C C Camargo
Anabolic androgenic steroids (AAS) are recommended for therapeutic clinic, but their use has increased in recent decades for aesthetic reasons. No study has evaluated the impact of AAS in the fallopian tube, after treatment and recovery periods. Herein, the aim of study was to investigate the effects of Nandrolone Decanoate (ND), administered in different doses (1.87; 3.75; 7.5 and 15 mg/kg) on the ampulla of the fallopian tube in rats, following post-treatment (PT; 15 consecutive days) and post-recovery (PR; 30 consecutive days) periods...
February 2018: Tissue & Cell
Carlos E Cuervo-Lozano, Adolfo Soto-Domínguez, Odila Saucedo-Cárdenas, Roberto Montes-de-Oca-Luna, Sergio Alonso-Romero, María Del Consuelo Mancías-Guerra, Eduardo Álvarez-Lozano
Treatment of massive bone defects is one of the most difficult problems to solve in orthopedics. At present, there is no consensus on the best way to resolve these problems. The aim of our study was to evaluate the effect of a three-dimensional bioimplant over massive bone defects, and to analyse if it improves the speed and quality of integration in recipient bone compared to allograft treatment. Fifteen female lambs with massive bone defects, surgically created in their tibias, were randomly divided into three groups of five lambs each: Group I -treated with the bioimplant; Group 2 -treated with the bioimplant plus nucleated cells of autologous bone marrow; Group 3 -treated with a frozen allograft...
February 2018: Tissue & Cell
Balbina J Plotkin, James W Davis, Luigi Strizzi, Peter Lee, Jayme Christoffersen-Cebi, Joan Kacmar, Orlando J Rivero, Norhan Elsayed, Nicholas Zanghi, Brent Ito, Ira M Sigar
The center of tumors, stem cell niches and mucosal surfaces all represent areas of the body that are reported to be anoxic. However, long-term study of anoxic cell physiology is hindered by the lack of a sustainable method permitting cell cultivation in the complete absence of oxygen. A novel methodology was developed that enabled anoxic cell cultivation (17d maximum time tested) and cell passage. In the absence of oxygen, cell morphology is significantly altered. All cells tested exhibited morphologic changes, i...
February 2018: Tissue & Cell
Papori Sharma, A S Yadav, N L Selokar, Dharmendra Kumar, S S Dhaka, P S Yadav
The somatic cells having higher levels of DNA methylation and reducing it in donor cells before nuclear transfer (NT) by treating them with chemicals, may improve cloning efficiency of NT embryos by providing donor cells with similar epigenetic characteristics as in vivo embryos. Therefore, the present study was planned to understand mechanism of epigenetic changes in donor cells (buffalo fibroblasts) treated with different concentration of sodium butyrate (NaBu)-a chromatin remodeling agent. The cultured fibroblasts purity and lineage were confirmed by fibroblast specific protein and gene markers (Vimentin, Tubulin and Cytokeratin) at different passages using immuno-staining and qPCR respectively...
February 2018: Tissue & Cell
Raquel V G de Castro, Mariana R Tavares, Fabiana F Bressan, Naira C G Pieri, Amanda Baracho Trindade Hill, Aline F Souza, Nathan da R N Cruz, Daniele S Martins, Carlos E Ambrósio, Flávio V Meirelles, Joaquim M Garcia
Skin is an extensive and easily accessible organ possessing various cell types that are constantly renewed. Previous studies have suggested the presence of a stem cell niche at the bulge region of the hair follicle, which contains cells positive for CD200 and CD34. Thus, this study sought to identify these cell populations in canine skin cells using the following methods 1- collecting samples of adult and fetal skin and isolating and culturing these cells using a method of simple enzymatic digestion and 2- testing the cell cultures for CD200 and CD34 in vitro and comparing them with skin tissue samples (in situ)...
February 2018: Tissue & Cell
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