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Tissue & Cell

Elena V Polishchuk, Roman S Polishchuk
Labeling of specific molecules for electron microscopy provides an extremely powerful tool to investigate subcellular distribution of proteins, lipids and sugars with resolution of a few nanometers. Moreover, this technology offers a unique opportunity to see how the pattern of these molecules changes in different physiological or pathological conditions. Pre-embedding labeling evolved as one of the easiest method for molecule detection in electron microscopy. It was named "pre-embedding" because the procedure involves a labeling step before the embedding of the specimen in the resin, its subsequent sectioning and analysis of sections under the electron microscope...
November 20, 2018: Tissue & Cell
Caroline Kizilyaprak, York-Dieter Stierhof, Bruno M Humbel
Volume microscopy has become an important method in cellular biology. In contrast to tedious serial sectioning volumes can now far more conveniently be obtained with serial-block face and focussed ion beam scanning electron microscopy. Serial-block face scanning electron microscopy is the instrument of choice for large volumes whereas focussed ion beam scanning electron microscopy has its merits in high voxel resolution. These aspects are discussed along with some specific applications of a focussed ion beam scanning electron microscope...
September 26, 2018: Tissue & Cell
Jéssica Costa, Rute Pereira, Jorge Oliveira, Ângela Alves, Ângela Marques-Magalhães, Amaro Frutuoso, Carla Leal, Nuno Barros, Rui Fernandes, Diogo Queiroz Almeida, Márcia Barreiro, Alberto Barros, Mário Sousa, Rosália Sá
The human oocyte zona pellucida (ZP) is made of four glycoproteins, ZP1-ZP4. Recently, the prostate adenocarcinoma and prostate cancer PC3 cell-line were shown to express the human oocyte ZP3 glycoprotein, which was evaluated in a single report subject to patent. To further clarify whether oocyte zona pellucida glycoproteins are expressed in prostate cancer tissue and PC3-cells, in this report we evaluated protein expression of the four ZP glycoproteins in normal prostate tissue, prostate adenocarcinoma tissue and PC3-cells, and performed quantitative mRNA expression of the four ZP glycoproteins in the PC3 cell-line...
December 2018: Tissue & Cell
B N Singh, K Pramanik
Lack of potential regenerative medicine to reconstruct damaged cartilage tissue has accelerated investigation and development of potential biomaterial for cartilage tissue engineering. In this study, we fabricated micron-sized non-mulberry silk fibroin fiber (SFF) using N,N-Dimethylacetamide (DMAC )/10% LiBr solution and further used to develop SFF reinforced chitosan(CH) based porous scaffold with desired pore size, porosity, swelling and structural stability. The developed scaffold was characterized for its various physico-chemical, mechanical and biological properties...
December 2018: Tissue & Cell
Rafael Monteiro Fernandes, Priscila Cunha Nascimento, Leonardo Oliveira Bittencourt, Ana Carolina Alves de Oliveira, Bruna Puty, Luana Ketlen Reis Leão, Luanna Melo Pereira Fernandes, Sabrina Cartágenes, Cristiane Socorro Ferraz Maia, Rafael Rodrigues Lima
Ethanol (EtOH) consumption is a risk factor for central nervous system damage, especially during adolescence. This study aimed to investigate the possible effects of chronic EtOH forced administration on gray and white matter of the spinal cord, from adolescence to adulthood. For this, male Wistar rats were administered EtOH by gavage (6.5 g/kg/day; 22.5% w/v) from the 35th to the 90th day of life, while control animals received only distilled water. After exposure, animals were euthanized and their spinal cords processed to obtain cervical and thoracic segments for histological analyses...
December 2018: Tissue & Cell
Xianxian Chen, Qinqin Wei, Jing Wang, Fei Peng, En Li, YongKang Zhou, Shengzhou Zhang
The Chinese alligator (Alligator sinensis) is an endemic rare crocodilian species in China. In this study, we investigated the cytochemical patterns of peripheral blood cells in Chinese alligators for the first time by a range of cytochemical staining techniques including periodic acid-Schiff(PAS), sudan black B(SBB), peroxidase(POX), alkaline phosphatase(AKP), acid phosphatase(ACP), chloroacetic acid AS-D naphthol esterase(AS-D) and α-naphthol acetate esterase(α-NAE) staining. Erythrocytes were positive for PAS, and negative for all the other staining; heterophils were strongly positive for SBB, POX, ACP, AKP, AS-D and α-NAE, while weakly positive for PAS staining; eosinophils were strongly positive for PAS, POX, AKP, ACP and AS-D, and weakly positive for SBB and α-NAE staining; basophils were strongly positive for PAS, positive for POX, ACP, AKP and α-NAE, and weakly positive for AS-D, while negative for SBB staining; monocytes were weakly positive for PAS, ACP, AKP and α-NAE, while negative for SBB, POX and AS-D staining; lymphocytes were weakly positive for PAS and α-NAE, negative for all the other staining; thrombocytes were weakly positive for PAS, and negative for all the other staining...
December 2018: Tissue & Cell
Xiaofeng Guo, Dongmei Zhang, Xing Zhang, Jiawei Jiang, Pengfei Xue, Chunshuai Wu, Jinlong Zhang, Guohua Jin, Zhen Huang, Jian Yang, Xinhui Zhu, Wei Liu, Guanhua Xu, Zhiming Cui, Guofeng Bao
Fibroblast-like synoviocytes (FLSs) play an essential role in rheumatoid arthritis (RA) by promoting synovitis, pannus growth and cartilage/bone destruction. Increased proliferation, migration and invasion of FLSs greatly contribute to RA initiation and progression. Dual-specificity tyrosine-regulated kinase 1A (Dyrk1A), a serine/threonine kinase, regulates MAPK pathway activation, and governs the proliferation and differentiation of neuronal progenitor cells and cancer cells. Till now, the expression and possible function of Dyrk1A in RA FLSs have not been explored...
December 2018: Tissue & Cell
Razieh Dalirfardouei, Khadijeh Jamialahmadi, Elahe Mahdipour
BACKGROUND: Mesenchymal stem cells (MSCs) are currently the most promising candidates in regenerative medicine. Nonetheless, there are several limitations associated with the MSC tissue source such as infrequent and invasive bone marrow sampling methods. To overcome these limitations, we have procured MSCs from the menstrual blood (MenSCs) as a non-invasive source using a straightforward and cost-effective method. Moreover, we isolated MenSCs-derived exosomes as a safe and highly effective approach to exert the paracrine effects of MSCs...
December 2018: Tissue & Cell
Elham Abedin, Roya Lari, Nasser Mahdavi Shahri, Masoud Fereidoni
Extracellular matrix (ECM) controls cellular behaviors such as proliferation, migration, and differentiation. The decellularized matrix of mammalian tissues has been used as a scaffold for the repair and reconstruction of tissue defects. In this study, for the first time demineralized and decellularized human epiphyseal bone matrix was used as a scaffold for a bone generation. Human epiphyseal bone was demineralized by hydrochloric acid and then decellularized by three methods of physical (slow freezing and snap freeze-thaw), enzymatic (trypsin 0...
December 2018: Tissue & Cell
Osamu Kobayashi, Shigekazu Tomizuka, Shota Shimizu, Ryuichiro Machida
Spermatogenesis in the freshwater pearl mussel Margaritifera laevis was investigated using light and electron microscopy. The testes of M. laevis are composed of numerous acini. We observed type A spermatogonia, large cells of irregular shape, solely near the acinus basal lamina. Type A spermatogonia proliferate and become type B spermatogonia, which are also irregular in shape and form clusters of germ cells of the same developmental stage. The numerous clusters differ with respect to developmental stage and are arranged randomly along the acinus periphery...
December 2018: Tissue & Cell
Arup Mistri, Usha Kumari, Swati Mittal, Ajay Kumar Mittal
The present study is concerned with the expression and localization of nitric oxide synthase (NOS) isoforms, nNOS, eNOS and iNOS in the epidermis and the gill epithelium of Chaca chaca by means of immunohistochemical techniques. nNOS immunoreactivity was observed in the outer layer epithelial cells of the epidermis, outer epithelium of gill filaments at their distal regions and in between the secondary lamellae. iNOS positive cells were observed at intervals in the epidermis from basal layer to superficial layer, in outer layers of epithelium of the gill filament and in epithelium of the secondary lamellae...
December 2018: Tissue & Cell
Akram Al Abbar, Norshariza Nordin, Nadiah Ghazalli, Syahril Abdullah
Induced pluripotent stem cells (iPSCs) have great potentials for regenerative medicine. However, serious concerns such as the use of the viral-mediated reprogramming strategies and exposure of iPSCs to animal products from feeder cells and serum-containing medium have restricted the application of iPSCs in the clinics. Therefore, the generation of iPSCs with minimal viral integrations and in non-animal sourced and serum-free medium is necessary. In this report, a polycistronic lentiviral vector carrying Yamanaka's factors was used to reprogram mouse fibroblasts into iPSCs in feeder- and xeno-free culture environment...
December 2018: Tissue & Cell
Wissam Hamidatou Khati, Elara N Moudilou, Jean-Marie Exbrayat, Sadjia Hammouche
Gerbillus tarabuli is a nocturnal seasonal breeder desert rodent with a main breeding season in spring and summer, and sexual quiescence in winter. This species is an interesting model for studying testis function in rodents. Therefore, the present study was performed firstly to investigate the stages of seminiferous epithelium cycle of Gerbillus tarabuli with a histological, morphometric and statistical study. And secondly to investigate the expression and possible variations in cellular distribution of RFamide-related peptide-3 (RFRP-3) - the mammalian ortholog of avian gonadotropin-inhibitory hormone (GnIH) - during seminiferous epithelium cycle using immunohistochimestry...
December 2018: Tissue & Cell
Anastasiia Kosykh, Arkadii Beilin, Kirill Sukhinich, Ekaterina Vorotelyak
Neural crest stem cells that located in the postnatal hair follicle (HF-NCSC) are considered a promising tool for treatment of nervous system diseases and injuries. It is well known that HF-NCSC can be used in the spinal cord and sciatic nerve reparation but their ability to restore brain structures is poorly studied. In this article we are investigating the interaction between HF-NCSC and a nerve tissue (embryonic and adult). We have found out that HF-NCSC isolated from adult mice grow and differentiate in accordance with the mouse embryo developmental stage when co-cultured with the embryonic nerve tissue...
October 2018: Tissue & Cell
Hoda H Anan, Rania A Zidan, Samia A Abd El-Baset, Manar M Ali
Despite its wide range of application, cyclophosphamide (CP) exhibits a wide range of adverse effects including reproductive toxicity. The emerging field of zinc oxide nanoparticles (ZnO NPs) therapy may provide a new hope for prevention of CP induced gonadal toxicity. Herein, we aim to investigate the possible role of ZnO NPs as a new strategy to protect against CP induced testicular injury. Sixty adult male albino rats were divided into 3 groups; control, CP treated and CP + ZnO NPs treated groups. CP group was injected with CP (5 mg/kg/day), whereas CP + ZnO NPs group was concomitantly injected with CP and ZnO NPs (5 mg/kg/day)...
October 2018: Tissue & Cell
Caterina Peña, Renata Vargas, Juan Pablo Hernandez-Fonseca, Jesús Mosquera
The up-regulation of HLA antigens is important during heart inflammatory events and myofibroblasts may modulate the expression of this molecule in tissues. To test this possibility, the effect of cardiac myofibroblast:macrophage contact and the production of myofibroblast inhibitor factor(s) on the macrophage HLA (Ia) expression were studied. Listeria monocytogenes-elicited Ia + peritoneal macrophages (high Ia expression) were co-cultured with cardiac myofibroblasts for 3 and 7 days (myofibroblast contact)...
October 2018: Tissue & Cell
Haifeng Fu, Feiyue Xing, Yi Lv, Boning Zeng, Pengtao You, Jing Liu
The Notch signaling pathway plays a key role in cell proliferation and development that is closely related to an inverted CCAAT box binding protein (ICBP90), but little is known about whether there is a correlation between Notch signaling and ICBP90. The aim of the current study was to elucidate this. MTT assay and flow cytometry were used to determine the proliferation, cell cycle and apoptosis of HepG2 or Hepa1-6 cells treated by N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT), a specific inhibitor of the Notch pathway...
October 2018: Tissue & Cell
Quanwei Wei, Nazar Ali Korejo, Jingle Jiang, Mulin Xu, Kaizhi Zheng, Dagan Mao, Fangxiong Shi
Acute gastric mucosal injuries are serious clinical problems worldwide and are principally found with different types of stresses in animals. A constant challenge is to find original plant products that can combat stress. In the present study, we examined the effects of big-leaf mulberry extracts on stomach injury, and the activity of nitric oxide synthases (NOS) and total antioxidant activity (TAO) in the gastric mucosae of mice during water immersion and restraint stress (WIRS). Our data showed that WIRS-exposed mice produced several injuries and showed an enhanced iNOS, reduced eNOS activity, and decreased TAO activity in the stomach, whereas pretreatment with big-leaf mulberry extracts increased TAO activitiy...
October 2018: Tissue & Cell
Toshihiko Fukuzawa, Yutaka Kikuchi
Isolation and culture of Xenopus laevis neural tubes resulted in differentiation of melanophores and iridophores from neural crest cells; the differentiated melanophores and iridophores were then maintained in culture for more than 6 months. Guanosine has been reported to promote reflecting platelet formation in melanin-producing pigment cells; however, the process of pigment organellogenesis is still unclear. In the present study, unusual light-reflecting pigment cells were observed upon addition of guanosine to the neural tube culture system, which contained melanosomes specific to melanophores, and reflecting platelets specific to iridophores...
October 2018: Tissue & Cell
Mirna Luciano de Gois da Silva, George Laylson da Silva Oliveira, Dayseanny de Oliveira Bezerra, Hermínio José da Rocha Neto, Matheus Levi Tajra Feitosa, Napoleão Martins Argôlo Neto, Marcia Dos Santos Rizzo, Maria Acelina Martins de Carvalho
Cell replacement through neural stem cells has been a promising alternative therapy for neurodegenerative diseases. It was evaluated the possible protect and/or prevent role of neurospheres in experimental models of epilepsy by the use of biomarkers of oxidative stress and histopathological analysis. After 1 h of the epileptic inductions by pilocarpine, pentylenotetrazole and picrotoxin, rats were infused with a suspension of 2 × 106 cells/0.25 mL, marked with Qtracker® 655, via caudal vein. In the control group epilepsy was not induced, but received the cell infusion under the same conditions of other groups...
October 2018: Tissue & Cell
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