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Tissue & Cell

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https://www.readbyqxmd.com/read/28087142/ultrastructural-characteristics-of-the-mature-spermatozoon-of-the-digenean-sclerodistomum-italicum-stossich-1893-hemiuroidea-sclerodistomidae-intestinal-parasite-of-hypocanthus-amia-teleostei-carangidae
#1
Papa Ibnou Ndiaye, Yann Quilichini, Adji Mama Marigo, Cheikh Tidiane Bâ, Vasyl V Tkach, Bernard Marchand
The mature spermatozoon of Sclerodistomum italicum is filiform, tapered at both ends and shows the following features: 2 axonemes of the 9+"1" pattern of the Trepaxonemata, mitochondrion, nucleus and parallel cortical microtubules. The specific features of the spermatozoon of S. italicum include the simultaneous presence of two types of extramembranous ornamentations, the presence of short cortical microtubules in the anterior part of the spermatozoon and the presence of only one bundle of cortical microtubules in the median part of the spermatozoon...
December 30, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28073590/objective-detection-of-apoptosis-in-rat-renal-tissue-sections-using-light-microscopy-and-free-image-analysis-software-with-subsequent-machine-learning-detection-of-apoptosis-in-renal-tissue
#2
Nayana Damiani Macedo, Aline Rodrigues Buzin, Isabela Bastos Binotti Abreu de Araujo, Breno Valentim Nogueira, Tadeu Uggere de Andrade, Denise Coutinho Endringer, Dominik Lenz
OBJECTIVE: The current study proposes an automated machine learning approach for the quantification of cells in cell death pathways according to DNA fragmentation. METHODS: A total of 17 images of kidney histological slide samples from male Wistar rats were used. The slides were photographed using an Axio Zeiss Vert.A1 microscope with a 40x objective lens coupled with an Axio Cam MRC Zeiss camera and Zen 2012 software. The images were analyzed using CellProfiler (version 2...
December 28, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28088324/protective-effect-of-sugar-cane-extract-against-dextran-sulfate-sodium-induced-colonic-inflammation-in-mice
#3
Bin Wang, Yansen Li, Masami Mizu, Toma Furuta, ChunMei Li
Sugar cane extract (SCE) exhibits various biological effects and has been reported to enhance animal growth performance. However, the effect of SCE on inflammation in animals is still obscure. To study the effects and underlying mechanism of SCE on dextran sulfate sodium (DSS)-induced colonic inflammation, forty female ICR mice (26.63±0.19g, 6-week-old) were assigned into four groups: a control group (Cont), a DSS-challenged group (DSS), a SCE-supplemented group (SCE), and a DSS+SCE group (DSS+SCE). Mice in Cont group and DSS group were fed basic diet and other mice received 1% SCE supplemented in basic diet from 6-week to 8-week-old...
December 26, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28049551/beta-catenin-can-induce-hair-follicle-stem-cell-differentiation-into-transit-amplifying-cells-through-c-myc-activation
#4
Qiong Shen, Weirong Yu, Yong Fang, Min Yao, Penggao Yang
Hair follicle stem cells play important roles in maintaining homeostasis and skin tissue self-renewal. Transit-amplifying cells represent the transition of cells from hair follicle stem cells into differentiated epidermal cells. Thus far, the signaling pathway and the molecular biological mechanism that regulate the proliferation and differentiation of hair follicle stem cells remain unclear. In this paper, we studied the relationship between β-catenin and c-myc during the process of the differentiation of hair follicle stem cells into transit-amplifying cells...
December 21, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28041835/comparison-of-cardiac-stem-cell-sheets-detached-by-versene-solution-and-from-thermoresponsive-dishes-reveals-similar-properties-of-constructs
#5
Konstantin V Dergilev, Pavel I Makarevich, Zoya I Tsokolaeva, Maria A Boldyreva, Irina B Beloglazova, Ekaterina S Zubkova, Mikhail Yu Menshikov, Yelena V Parfyonova
Cell sheets (CS) from c-kit+ cardiac stem cell (CSC) hold a potential for application in regenerative medicine. However, manufacture of CS may require thermoresponsive dishes, which increases cost and puts one in dependence on specific materials. Alternative approaches were established recently and we conducted a short study to compare approaches for detachment of CS from c-kit+ CSC. Our in-house developed method using chelation by Versene solution was compared to UpCell™ thermoresponsive plates in terms of CSC proliferation, viability, gap junction formation and engraftment in a model of myocardial infarction...
December 16, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28011039/the-direction-of-human-mesenchymal-stem-cells-into-the-chondrogenic-lineage-is-influenced-by-the-features-of-hydrogel-carriers
#6
A Hansson, A Wenger, H Barreto Henriksson, S Li, B R Johansson, H Brisby
Low back pain is a major public health issue in the Western world, one main cause is believed to be intervertebral disc (IVD) degeneration. To halt/diminish IVD degeneration, cell therapy using different biomaterials e.g. hydrogels as cell carriers has been suggested. In this study, two different hydrogels were examined (in vitro) as potential cell carriers for human mesenchymal stem cells (hMSCs) intended for IVD transplantation. The aim was to investigate cell-survival and chondrogenic differentiation of hMSCs when cultured in hydrogels Puramatrix(®) or Hydromatrix(®) and potential effects of stimulation with growth hormone (GH)...
December 13, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28007425/different-golgi-ultrastructure-across-species-and-tissues-implications-under-functional-and-pathological-conditions-and-an-attempt-at-classification
#7
REVIEW
Alexander A Mironov, Irina S Sesorova, Elena V Seliverstova, Galina V Beznoussenko
The Golgi complex (GC) is the central station of the secretory pathway, through which several paths of intracellular transport are connected. The main function of the GC is glycosylation of proteins and lipids, and their subsequent sorting. The structure of the GC is extremely complicated, although in general it is unbelievably similar across different cells types and under different functional and pathological conditions. However, there are also a lot of differences between the GCs in different cells and under different normal and pathological conditions...
December 10, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28011040/cranial-nerves-in-the-australian-lungfish-neoceratodus-forsteri-and-in-fossil-relatives-osteichthyes-dipnoi
#8
A Kemp
Three systems, two sensory and one protective, are present in the skin of the living Australian lungfish, Neoceratodus forsteri, and in fossil lungfish, and the arrangement and innervation of the sense organs is peculiar to lungfish. Peripheral branches of nerves that innervate the sense organs are slender and unprotected, and form before any skeletal structures appear. When the olfactory capsule develops, it traps some of the anterior branches of cranial nerve V, which emerged from the chondrocranium from the lateral sphenotic foramen...
December 9, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28034554/biological-characters-of-human-dermal-fibroblasts-derived-from-foreskin-of-male-infertile-patients
#9
Fang Fang, Ke Ni, Yiting Cai, Zhen Ye, Jin Shang, Shiliang Shen, Chengliang Xiong
Dermal fibroblasts play a vital role in maintaining skin function. They not only synthesize and secrete extracellular matrix molecules, but also produce a complex mixture of bioactive factors, which both contribute to immune regulation and wound healing. Fibroblasts isolated from skin tissue exhibit wide range of potentials, especially in regenerative medicine. The use of fibroblast cultures for medical purposes requires standardization of cell preparations. To achieve this, we isolated and characterized dermal fibroblasts from human foreskin with a standardized method...
December 8, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/27939404/proliferation-and-apoptosis-in-subcutaneous-adipose-tissue-of-lactating-cows-with-different-genetic-merit-for-milk-yield
#10
Monica Colitti, Nataliya Pošćić, Bruno Stefanon
The aim of this study was to investigate the adipocyte size and fate in subcutaneous fat (scAT) of cows diverging for genetic merit at mid lactation stage, when anabolic activity increases and animals are in a state of positive energy balance. Twenty mid lactation cows (180±20days in milk) grouped according to the Estimated Breeding Values (EBV) for milk yield in plus (EBVp) and minus (EBVm) variants were selected. Average of adipocytes area, proliferation and apoptotic labelling index as well as DLK-1 expression, a marker of pre-adipocytes, were immunohistochemically evaluated in scAT biopsies...
November 24, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28034555/impact-of-dengue-virus-serotype-denv-2-infection-on-liver-of-balb-c-mice-a-histopathological-analysis
#11
S Sakinah, Sivan Padma Priya, Sharmilah Kumari, Fatin Amira, Poorani K, Hiba Alsaeedy, Mok Pooi Ling, Hui-Yee Chee, Akon Higuchi, Abdullah A Alarfaj, Murugan A Munusamy, Kadarkarai Murugan, Che Norma Mat Taib, Palanisamy Arulselvan, Mariappan Rajan, Vasantha Kumari Neela, Rukman Awang Hamat, Giovanni Benelli, S Suresh Kumar
In this research, we characterized the histopathological impact of dengue virus (serotype DENV-2) infection in livers of BALB/c mice. The mice were infected with different doses of DENV-2 via intraperitoneal injection and liver tissues were processed for histological analyses and variation was documented. In the BALB/c mouse model, typical liver tissues showed regular hepatocyte architecture, with normal endothelial cells surrounding sinusoid capillary. Based on histopathological observations, the liver sections of BALB/c mice infected by DENV-2 exhibited a loss of cell integrity, with a widening of the sinusoidal spaces...
November 23, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/27894594/alterations-of-golgi-organization-in-alzheimer-s-disease-a-cause-or-a-consequence
#12
REVIEW
Inmaculada Ayala, Antonino Colanzi
The Golgi apparatus is a central organelle of the secretory pathway involved in the post-translational modification and sorting of lipids and proteins. In mammalian cells, the Golgi apparatus is composed of stacks of cisternae organized in polarized manner, which are interconnected by membrane tubules to constitute the Golgi ribbon, located in the proximity of the centrosome. Besides the processing and transport of cargo, the Golgi complex is actively involved in the regulation of mitotic entry, cytoskeleton organization and dynamics, calcium homeostasis, and apoptosis, representing a signalling platform for the control of several cellular functions, including signalling initiated by receptors located at the plasma membrane...
November 19, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/27890351/unilaminar-follicular-cells-transiently-express-galectin-3-during-ovarian-folliculogenesis-in-pigs
#13
Seung-Dam Heo, Changnam Park, Jeongtae Kim, Meejung Ahn, Taekyun Shin
The localization of galectin-3, a β-galactoside-binding animal lectin, was immunohistochemically studied in the ovaries of pigs to determine its expression in ovarian folliculogenesis. Various stages of ovarian follicles were identified in the ovaries of adult pigs. Galectin-3 was immunostained in the squamous follicular cells surrounding oocytes in primordial follicles and in the unilaminar granulosa cells of primary follicles, but not in oocytes of multilaminar follicles (including primary, secondary, and tertiary Graafian follicles)...
November 17, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/27884532/morphometric-analysis-of-the-folliculostellate-cells-and-luteinizing-hormone-gonadotropic-cells-of-the-anterior-pituitary-of-the-men-during-the-aging-process
#14
Jovana Čukuranović Kokoris, Ivan Jovanović, Vukica Pantović, Miljan Krstić, Milica Stanojković, Verica Milošević, Slađana Ugrenović, Vesna Stojanović
The aim of this research was to quantify the changes in the morphology and density of the anterior pituitary folliculostellate (FS) and luteinizing hormone (LH) cells. Material was tissue of the pituitary gland of the 14 male cadavers. Tissue slices were immunohistochemically stained with monoclonal anti-LH antibody and polyclonal anti-S100 antibody for the detection of LH and FS cells, respectively. Digital images of the stained slices were afterwards morphometrically analyzed by ImageJ. Results of the morphometric analysis showed significant increase of the FS cells volume density in cases older than 70 years...
November 17, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/27890350/the-histophysiology-and-pathophysiology-of-the-peritoneum
#15
REVIEW
J O A M van Baal, K K Van de Vijver, R Nieuwland, C J F van Noorden, W J van Driel, A Sturk, G G Kenter, L G Rikkert, C A R Lok
The peritoneum is an extensive serous organ with both epithelial and mesenchymal features and a variety of functions. Diseases such as inflammatory peritonitis and peritoneal carcinomatosis can induce disturbance of the complex physiological functions. To understand the peritoneal response in disease, normal embryonic development, anatomy in healthy conditions and physiology of the peritoneum have to be understood. This review aims to summarize and discuss the literature on these basic peritoneal characteristics...
November 13, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/27887732/expressions-of-pathologic-markers-in-prp-based-chondrogenic-differentiation-of-human-adipose-derived-stem-cells
#16
Arezou Pakfar, Shiva Irani, Hana Hanaee-Ahvaz
BACKGROUND: Optimization of the differentiation medium through using autologous factors such as PRP is of great consideration, but due to the complex, variable and undefined composition of PRP on one hand and lack of control over the absolute regulatory mechanisms in in vitro conditions or disrupted and different mechanisms in diseased tissue microenvironments in in vivo conditions on the other hand, it is complicated and rather unpredictable to get the desired effects of PRP making it inevitable to monitor the possible pathologic or undesired differentiation pathways and therapeutic effects of PRP...
November 12, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/27865438/evaluation-of-bone-marrow-derived-mesenchymal-stem-cells-for-full-thickness-wound-healing-in-comparison-to-tissue-engineered-chitosan-scaffold-in-rabbit
#17
Mohammad Hossein Rajabian, Gholam Hossein Ghorabi, Bita Geramizadeh, Safoura Sameni, Maryam Ayatollahi
BACKGROUND: Chronic wounds present a major challenge in modern medicine. Even under optimal conditions, the healing process may lead to scarring and fibrosis. The ability of mesenchymal stem cells (MSCs) to differentiate into other cell types makes these cells an attractive therapeutic tool for cell transplantation. Both tissue-engineered construct and MSC therapy are among the current wound healing procedures and potential care. Chitosan has been widely applied in tissue engineering because of its biocompatibility and biodegradability...
November 9, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/27837912/cold-inducible-rna-binding-protein-in-mouse-mammary-gland-development
#18
Daniel A Lujan, Selina Garcia, Jennifer Vanderhoof, Joshua Sifuentes, Yekaterina Brandt, Yuehan Wu, Xun Guo, Therese Mitchell, Tamara Howard, Helen J Hathaway, Rebecca S Hartley
RNA binding proteins (RBPs) regulate gene expression by controlling mRNA export, translation, and stability. When altered, some RBPs allow cancer cells to grow, survive, and metastasize. Cold-inducible RNA binding protein (CIRP) is overexpressed in a subset of breast cancers, induces proliferation in breast cancer cell lines, and inhibits apoptosis. Although studies have begun to examine the role of CIRP in breast and other cancers, its role in normal breast development has not been assessed. We generated a transgenic mouse model overexpressing human CIRP in the mammary epithelium to ask if it plays a role in mammary gland development...
December 2016: Tissue & Cell
https://www.readbyqxmd.com/read/27823763/structure-of-neuro-endocrine-and-neuro-epithelial-interactions-in-human-foetal-pancreas
#19
Yuliya Krivova, Alexandra Proshchina, Valeriy Barabanov, Olga Leonova, Sergey Saveliev
In the pancreas of many mammals including humans, endocrine islet cells can be integrated with the nervous system components into neuro-insular complexes. The mechanism of the formation of such complexes is not clearly understood. The present study evaluated the interactions between the nervous system components, epithelial cells and endocrine cells in the human pancreas. Foetal pancreas, gestational age 19-23 weeks (13 cases) and 30-34 weeks (7 cases), were studied using double immunohistochemical labeling with neural markers (S100 protein and beta III tubulin), epithelial marker (cytokeratin 19 (CK19)) and antibodies to insulin and glucagon...
December 2016: Tissue & Cell
https://www.readbyqxmd.com/read/27823762/alterations-in-oviductal-cilia-morphology-and-reduced-expression-of-axonemal-dynein-in-diabetic-nod-mice
#20
Stephanie L Shorter, Ahmad J H Albaghdadi, Frederick W K Kan
In the present study, we examined the morphology of cilia and expression of the dynein intermediate chain 2 (DNAI2) in the oviduct of non-obese diabetic (NOD) mice. Results obtained with immunohistochemistry showed that DNAI2 expression was reduced in oviducts of diabetic NOD (dNOD) mice, as compared to that observed in the normoglycemic NOD (cNOD) group, especially in the acyclic dNOD mice. Oviductal cilia of dNOD mice appeared to be reduced in number. Results obtained with Western blot analysis revealed that the expression of DNAI2 protein was significantly less in oviducts of dNOD mice as compared to that of cNOD mice corroborating the results obtained with immunohistochemistry...
December 2016: Tissue & Cell
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