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Tissue & Cell

Inmaculada Ayala, Antonino Colanzi
The Golgi apparatus is a central organelle of the secretory pathway involved in the post-translational modification and sorting of lipids and proteins. In mammalian cells, the Golgi apparatus is composed of stacks of cisternae organized in polarized manner, which are interconnected by membrane tubules to constitute the Golgi ribbon, located in the proximity of the centrosome. Besides the processing and transport of cargo, the Golgi complex is actively involved in the regulation of mitotic entry, cytoskeleton organization and dynamics, calcium homeostasis, and apoptosis, representing a signalling platform for the control of several cellular functions, including signalling initiated by receptors located at the plasma membrane...
November 19, 2016: Tissue & Cell
Seung-Dam Heo, Changnam Park, Jeongtae Kim, Meejung Ahn, Taekyun Shin
The localization of galectin-3, a β-galactoside-binding animal lectin, was immunohistochemically studied in the ovaries of pigs to determine its expression in ovarian folliculogenesis. Various stages of ovarian follicles were identified in the ovaries of adult pigs. Galectin-3 was immunostained in the squamous follicular cells surrounding oocytes in primordial follicles and in the unilaminar granulosa cells of primary follicles, but not in oocytes of multilaminar follicles (including primary, secondary, and tertiary Graafian follicles)...
November 17, 2016: Tissue & Cell
Jovana Čukuranović Kokoris, Ivan Jovanović, Vukica Pantović, Miljan Krstić, Milica Stanojković, Verica Milošević, Slađana Ugrenović, Vesna Stojanović
The aim of this research was to quantify the changes in the morphology and density of the anterior pituitary folliculostellate (FS) and luteinizing hormone (LH) cells. Material was tissue of the pituitary gland of the 14 male cadavers. Tissue slices were immunohistochemically stained with monoclonal anti-LH antibody and polyclonal anti-S100 antibody for the detection of LH and FS cells, respectively. Digital images of the stained slices were afterwards morphometrically analyzed by ImageJ. Results of the morphometric analysis showed significant increase of the FS cells volume density in cases older than 70 years...
November 17, 2016: Tissue & Cell
J O A M van Baal, K K Van de Vijver, R Nieuwland, C J F van Noorden, W J van Driel, A Sturk, G G Kenter, L G Rikkert, C A R Lok
The peritoneum is an extensive serous organ with both epithelial and mesenchymal features and a variety of functions. Diseases such as inflammatory peritonitis and peritoneal carcinomatosis can induce disturbance of the complex physiological functions. To understand the peritoneal response in disease, normal embryonic development, anatomy in healthy conditions and physiology of the peritoneum have to be understood. This review aims to summarize and discuss the literature on these basic peritoneal characteristics...
November 13, 2016: Tissue & Cell
Arezou Pakfar, Shiva Irani, Hana Hanaee-Ahvaz
BACKGROUND: Optimization of the differentiation medium through using autologous factors such as PRP is of great consideration, but due to the complex, variable and undefined composition of PRP on one hand and lack of control over the absolute regulatory mechanisms in in vitro conditions or disrupted and different mechanisms in diseased tissue microenvironments in in vivo conditions on the other hand, it is complicated and rather unpredictable to get the desired effects of PRP making it inevitable to monitor the possible pathologic or undesired differentiation pathways and therapeutic effects of PRP...
November 12, 2016: Tissue & Cell
Mohammad Hossein Rajabian, Gholam Hossein Ghorabi, Bita Geramizadeh, Safoura Sameni, Maryam Ayatollahi
BACKGROUND: Chronic wounds present a major challenge in modern medicine. Even under optimal conditions, the healing process may lead to scarring and fibrosis. The ability of mesenchymal stem cells (MSCs) to differentiate into other cell types makes these cells an attractive therapeutic tool for cell transplantation. Both tissue-engineered construct and MSC therapy are among the current wound healing procedures and potential care. Chitosan has been widely applied in tissue engineering because of its biocompatibility and biodegradability...
November 9, 2016: Tissue & Cell
Daniel A Lujan, Selina Garcia, Jennifer Vanderhoof, Joshua Sifuentes, Yekaterina Brandt, Yuehan Wu, Xun Guo, Therese Mitchell, Tamara Howard, Helen J Hathaway, Rebecca S Hartley
RNA binding proteins (RBPs) regulate gene expression by controlling mRNA export, translation, and stability. When altered, some RBPs allow cancer cells to grow, survive, and metastasize. Cold-inducible RNA binding protein (CIRP) is overexpressed in a subset of breast cancers, induces proliferation in breast cancer cell lines, and inhibits apoptosis. Although studies have begun to examine the role of CIRP in breast and other cancers, its role in normal breast development has not been assessed. We generated a transgenic mouse model overexpressing human CIRP in the mammary epithelium to ask if it plays a role in mammary gland development...
November 5, 2016: Tissue & Cell
Stephanie L Shorter, Ahmad J H Albaghdadi, Frederick W K Kan
In the present study, we examined the morphology of cilia and expression of the dynein intermediate chain 2 (DNAI2) in the oviduct of non-obese diabetic (NOD) mice. Results obtained with immunohistochemistry showed that DNAI2 expression was reduced in oviducts of diabetic NOD (dNOD) mice, as compared to that observed in the normoglycemic NOD (cNOD) group, especially in the acyclic dNOD mice. Oviductal cilia of dNOD mice appeared to be reduced in number. Results obtained with Western blot analysis revealed that the expression of DNAI2 protein was significantly less in oviducts of dNOD mice as compared to that of cNOD mice corroborating the results obtained with immunohistochemistry...
October 29, 2016: Tissue & Cell
Yuliya Krivova, Alexandra Proshchina, Valeriy Barabanov, Olga Leonova, Sergey Saveliev
In the pancreas of many mammals including humans, endocrine islet cells can be integrated with the nervous system components into neuro-insular complexes. The mechanism of the formation of such complexes is not clearly understood. The present study evaluated the interactions between the nervous system components, epithelial cells and endocrine cells in the human pancreas. Foetal pancreas, gestational age 19-23 weeks (13 cases) and 30-34 weeks (7 cases), were studied using double immunohistochemical labeling with neural markers (S100 protein and beta III tubulin), epithelial marker (cytokeratin 19 (CK19)) and antibodies to insulin and glucagon...
October 26, 2016: Tissue & Cell
Laila Rashed, Doaa Mostafa Gharib, Rania Elsayed Hussein, Ola Tork, Azza Abusree
AIM: To study the effect of intravenous injection of bone marrow mesenchymal stem cells (BMMSCs), alone and combined with NO inducer in gastric ulcer healing in a rat model. METHODS: Rats were divided into controls, gastric ulcer, gastric ulcer receiving mesenchymal stem cells (MSCs), gastric ulcer receiving NO inducer (l-Arginine), gastric ulcer receiving MSCs plus NO inducer (l-Arginine) groups. MSCs were given in a dose of (10(6)cells) by intravenous injection...
September 28, 2016: Tissue & Cell
Maeve Long, Jeremy C Simpson
The Golgi complex is the central unit of the secretory pathway, modifying, processing and sorting proteins and lipids to their correct cellular localisation. Changes to proteins at the Golgi complex can have deleterious effects on the function of this organelle, impeding trafficking routes through it, potentially resulting in disease. It is emerging that several Rho GTPase proteins, namely Cdc42, RhoBTB3, RhoA and RhoD are at least in part localised to the Golgi complex, and a number of studies have shown that dysregulation of their levels or activity can be associated with cellular changes which ultimately drive cancer progression...
September 28, 2016: Tissue & Cell
Lizette du Plessis, John T Soley
Head shaping in mammalian sperm is regulated by a number of factors including acrosome formation, nuclear condensation and the action of the microtubular manchette. A role has also been suggested for the attendant Sertoli cells and the perinuclear theca (PT). In comparison, relatively little information is available on this topic in birds and the presence of a PT per se has not been described in this vertebrate order. This study revealed that a similar combination of factors contributed to head shaping in the ostrich, emu and rhea, although the Sertoli cells seem to play a limited role in ratites...
September 28, 2016: Tissue & Cell
H El-Tahry, Hany E Marei, A Shams, M El-Shahat, H Abdelaziz, M Abd El-Kader
No abstract text is available yet for this article.
September 22, 2016: Tissue & Cell
Susanne Smaardijk, Jialin Chen, Frank Wuytack, Peter Vangheluwe
Dysregulation of the Golgi/Secretory Pathway Ca(2+) transport ATPase SPCA2 is implicated in breast cancer. During lactation and in luminal breast cancer types, SPCA2 interacts with the plasma membrane Ca(2+) channel Orai1, promoting constitutive Ca(2+) influx, which is termed store independent Ca(2+) entry (SICE). The mechanism of SPCA2/Orai1 interaction depends on the N- and C-termini of SPCA2. These extensions may play a dual role in activating not only Orai1, but also Ca(2+) transport into the Golgi/secretory pathway, which we tested by investigating the impact of various SPCA2 N- and/or C-terminal truncations on SICE and Ca(2+) transport activity of SPCA2...
September 17, 2016: Tissue & Cell
Sae Bin Lee, A-Ram Lim, Dong Kyun Rah, Kyung Soo Kim, Hyun Jin Min
Heat shock protein 90 is a chaperone molecule that aids in proper folding of target proteins. Recently, heat shock protein 90 was found to play a role in would healing through regulation of fibroblast functions. The aim of the present study was to investigate the role of heat shock protein 90 in collagen synthesis in human dermal fibroblasts. The effects of transforming growth factor-β, 17-N-allylamino-17-demethoxygeldanamycin, and transfection of heat shock protein 90 were evaluated by real-time PCR, western blot, and immunofluorescence assays...
September 13, 2016: Tissue & Cell
Panuroot Aguilar, Veera Lertchirakarn
CD24 was suggested as a marker to SCAPs and has been reported for a decade. CD24 has been shown to involve stem cell activities such as self-renewal, proliferation and differentiation. However, the percentage variations of CD24 positive cells were reported among the studies. It is possible that this variation may affect these SCAPs behaviors. In this study, the variation was confirmed. To elucidate the influence of CD24 positive cells quantity on SCAPs stem cell behaviors, the 3 cell lines with the most maximum and the least numbers of CD24 positive cells (High-CD24 and Low-CD24 group) were selected to study...
October 2016: Tissue & Cell
Panarat Rattanawarawipa, Prasit Pavasant, Thanaphum Osathanon, Waleerat Sukarawan
Lithium Chloride (LiCl) has been used as a canonical Wnt pathway activator due to its ability to inhibit a glycogen synthase kinase-3. The aim of the present study was to investigate the effect of LiCl on cell proliferation and osteogenic differentiation in stem cells isolated from human exfoliated deciduous teeth (SHEDs). SHEDs were isolated and cultured in media supplemented with LiCl at 5, 10, or 20mM. The results demonstrated that LiCl significantly decreased SHEDs colony forming unit ability in a dose dependent manner...
October 2016: Tissue & Cell
L Pardyak, A Kaminska, J Galas, A Ptak, B Bilinska, M Kotula-Balak
We report the effects of polychlorinated napthalanes (PCNs) on the mRNA expression of estrogen-related receptors (ERRs) α, β and γ, calcium (Ca2+) concentration, and sex steroid secretion in mouse primary and tumor Leydig cells. The cells were exposed to a mixture of PCNs (10nM) alone or in combination with one of sex steroid receptor antagonists; 182,780 (ICI; 10μM); hydroxyflutamide (HF; 10(-4)M) and G-coupled estrogen receptor antagonist (G15; 10nM) respectively. The expression of mRNAs and protein for ERRα, β, and γ was detected in primary and tumor Leydig cells...
October 2016: Tissue & Cell
Carolyn K Novaleski, Emily E Kimball, Masanobu Mizuta, Bernard Rousseau
Clinical voice disorders pose significant communication-related challenges to patients. The purpose of this study was to quantify the rate of apoptosis and tumor necrosis factor-alpha (TNF-α) signaling in vocal fold epithelial cells in response to increasing time-doses and cycle-doses of vibration. 20 New Zealand white breeder rabbits were randomized to three groups of time-doses of vibration exposure (30, 60, 120min) or a control group (120min of vocal fold adduction and abduction). Estimated cycle-doses of vocal fold vibration were extrapolated based on mean fundamental frequency...
October 2016: Tissue & Cell
Jia Chen, Dan Zhang, Qin Li, Dan Yang, Zengjie Fan, Dongyang Ma, Liling Ren
The repair and reconstruction of large bone defects remains as a significant clinical challenge mainly due to the insufficient vascularization. The prefabrication of vascular network based on cell sheet technique brings a promising potential for sufficient vascularization due to rich extracellular matrix (ECM) of cell sheets. However, the effect of different cell sheet ECM micro-environment on the formation of a vascular network has not been well understood. Here our goal is to study the effect of different cell sheets on the formation of a vascular network...
October 2016: Tissue & Cell
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