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Tissue & Cell

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https://www.readbyqxmd.com/read/29429524/nintedanib-effects-on-delaying-cancer-progression-and-decreasing-cox-2-and-il-17-in-the-prostate-anterior-lobe-in-tramp-mice
#1
Letícia Ferreira Alves, Raquel Frenedoso da Silva, Valéria Helena Alves Cagnon
Prostate cancer is the most prevalent type of cancer in men around the world. Due to its high incidence, new therapies have been evaluated, including drugs capable of inhibiting the FGF/VEGF pathways, as Nintedanib. The aim herein was to evaluate the Nintedanib therapeutic effects on morphology and COX-2 and IL-17 levels in the prostate anterior lobe in different grades of the tumor progression in TRAMP mice. Animals were treated with Nintedanib at a dose of 10 mg/kg/day in initial and intermediate grades of tumor development...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429523/allicin-ameliorates-cognitive-impairment-in-app-ps1-mice-via-suppressing-oxidative-stress-by-blocking-jnk-signaling-pathways
#2
Hao Zhang, Ping Wang, Yixue Xue, Libo Liu, Zhen Li, Yunhui Liu
In middle and old age, Alzheimer's disease (AD) is a progressive neurodegenerative disorder of brain. As an increasingly aging population, AD represents a huge burden for the patients' family and the country. However, current therapeutical strategies have shown limited effectiveness. Allicin, which is the main composition of garlic, was reported to prevent the learning and memory impairment of AD mouse model. As the mechanism is not clear, in this study, we used the APP (amyloid precursor protein)/PS1 (presenilin 1) double transgenic mice, which express human mutant APP and PS1, to determine the protective effect of allicin on neurons...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429522/neurochemical-characteristics-of-calbindin-like-immunoreactive-coeliac-cranial-mesenteric-ganglion-complex-ccmg-neurons-supplying-the-pre-pyloric-region-of-the-porcine-stomach
#3
Katarzyna Palus, Michał Bulc, Marta Czajkowska, Bartosz Miciński, Jarosław Całka
The present study was designed to determine the distribution, morphology and co-localization of calbindin-D28k (CB) with other neuroactive substances in the coeliac-cranial mesenteric ganglion complex (CCMG) neurons supplying the prepyloric region of the porcine stomach. In all animals, a median laparotomy was performed and the fluorescent retrograde neuronal tracer Fast Blue was injected into the wall of the stomach prepyloric area. On the 28th day, all animals were euthanized and the CCMG complexes were then collected and processed for double-labelling immunofluorescence for CB and tyrosine hydroxylase (TH), galanin (GAL), somatostatin (SOM), leu 5-enkephalin (LENK), vasoactive intestinal peptide (VIP), substance P (SP) and cocaine- and amphetamine-regulated transcript peptide (CART), Immunohistochemistry revealed that 8...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429521/sex-steroid-receptors-profiling-is-influenced-by-nandrolone-decanoate-in-the-ampulla-of-the-fallopian-tube-post-treatment-and-post-recovery-analyses
#4
G H B Andrade, V A Simão, B R Souza, L G A Chuffa, I C C Camargo
Anabolic androgenic steroids (AAS) are recommended for therapeutic clinic, but their use has increased in recent decades for aesthetic reasons. No study has evaluated the impact of AAS in the fallopian tube, after treatment and recovery periods. Herein, the aim of study was to investigate the effects of Nandrolone Decanoate (ND), administered in different doses (1.87; 3.75; 7.5 and 15 mg/kg) on the ampulla of the fallopian tube in rats, following post-treatment (PT; 15 consecutive days) and post-recovery (PR; 30 consecutive days) periods...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429520/osteogenesis-induced-by-a-three-dimensional-bioimplant-composed-of-demineralised-bone-matrix-collagen-hydroxyapatite-and-bone-marrow-derived-cells-in-massive-bone-defects-an-experimental-study
#5
Carlos E Cuervo-Lozano, Adolfo Soto-Domínguez, Odila Saucedo-Cárdenas, Roberto Montes-de-Oca-Luna, Sergio Alonso-Romero, María Del Consuelo Mancías-Guerra, Eduardo Álvarez-Lozano
Treatment of massive bone defects is one of the most difficult problems to solve in orthopedics. At present, there is no consensus on the best way to resolve these problems. The aim of our study was to evaluate the effect of a three-dimensional bioimplant over massive bone defects, and to analyse if it improves the speed and quality of integration in recipient bone compared to allograft treatment. Fifteen female lambs with massive bone defects, surgically created in their tibias, were randomly divided into three groups of five lambs each: Group I -treated with the bioimplant; Group 2 -treated with the bioimplant plus nucleated cells of autologous bone marrow; Group 3 -treated with a frozen allograft...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429519/a-method-for-the-long-term-cultivation-of-mammalian-cells-in-the-absence-of-oxygen-characterization-of-cell-replication-hypoxia-inducible-factor-expression-and-reactive-oxygen-species-production
#6
Balbina J Plotkin, James W Davis, Luigi Strizzi, Peter Lee, Jayme Christoffersen-Cebi, Joan Kacmar, Orlando J Rivero, Norhan Elsayed, Nicholas Zanghi, Brent Ito, Ira M Sigar
The center of tumors, stem cell niches and mucosal surfaces all represent areas of the body that are reported to be anoxic. However, long-term study of anoxic cell physiology is hindered by the lack of a sustainable method permitting cell cultivation in the complete absence of oxygen. A novel methodology was developed that enabled anoxic cell cultivation (17d maximum time tested) and cell passage. In the absence of oxygen, cell morphology is significantly altered. All cells tested exhibited morphologic changes, i...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429518/epigenetic-status-of-buffalo-fibroblasts-treated-with-sodium-butyrate-a-chromatin-remodeling-agent
#7
Papori Sharma, A S Yadav, N L Selokar, Dharmendra Kumar, S S Dhaka, P S Yadav
The somatic cells having higher levels of DNA methylation and reducing it in donor cells before nuclear transfer (NT) by treating them with chemicals, may improve cloning efficiency of NT embryos by providing donor cells with similar epigenetic characteristics as in vivo embryos. Therefore, the present study was planned to understand mechanism of epigenetic changes in donor cells (buffalo fibroblasts) treated with different concentration of sodium butyrate (NaBu)-a chromatin remodeling agent. The cultured fibroblasts purity and lineage were confirmed by fibroblast specific protein and gene markers (Vimentin, Tubulin and Cytokeratin) at different passages using immuno-staining and qPCR respectively...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429517/in-vitro-identification-of-a-stem-cell-population-from-canine-hair-follicle-bulge-region
#8
Raquel V G de Castro, Mariana R Tavares, Fabiana F Bressan, Naira C G Pieri, Amanda Baracho Trindade Hill, Aline F Souza, Nathan da R N Cruz, Daniele S Martins, Carlos E Ambrósio, Flávio V Meirelles, Joaquim M Garcia
Skin is an extensive and easily accessible organ possessing various cell types that are constantly renewed. Previous studies have suggested the presence of a stem cell niche at the bulge region of the hair follicle, which contains cells positive for CD200 and CD34. Thus, this study sought to identify these cell populations in canine skin cells using the following methods 1- collecting samples of adult and fetal skin and isolating and culturing these cells using a method of simple enzymatic digestion and 2- testing the cell cultures for CD200 and CD34 in vitro and comparing them with skin tissue samples (in situ)...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429516/lipoic-acid-inhibited-desflurane-induced-hippocampal-neuronal-apoptosis-through-caspase3-and-nf-kappab-dependent-pathway
#9
Hui Zhao, Meimei Bu, Binglu Li, Yong Zhang
Desfluraneis a widely-used general anesthetics. However, recent reports showed its significant side effect in the nervous system. Desflurane could lead to the neuronal death and affect the working memory. Unfortunately, the mechanism underlying the action of desflurane is still not clear and there is still no potent medicine to prevent the lesion in the central nervous system caused by general anesthetics. In this study, we found α-lipoic acid, an antioxidant exerting protective effect on multiple cells tissues, could resist the neurotoxicity caused by desflurane exposure...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429515/mice-dental-pulp-and-periodontal-ligament-endothelial-cells-exhibit-different-proangiogenic-properties
#10
Mohammad Ali Saghiri, Armen Asatourian, Christine M Sorenson, Nader Sheibani
Dental pulp is a highly vascularized tissue with a high regenerative capacity. This is attributed to its unique blood supply and the presence of progenitor or postnatal dental pulp stem cells. Here we aimed to isolate and compare the angiogenic properties of endothelial cells (EC) prepared from mouse dental pulp and periodontal ligament (PDL). EC were isolated from 4-week-old wild type immorto mice. Mice were sacrificed and after mandible isolation, the molar and incisor teeth and the PDL from molar teeth were dissected...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429514/culturing-substrates-influence-the-morphological-mechanical-and-biochemical-features-of-lung-adenocarcinoma-cells-cultured-in-2d-or-3d
#11
Adriele Prina-Mello, Namrata Jain, Baiyun Liu, Jason I Kilpatrick, Melissa A Tutty, Alan P Bell, Suzanne P Jarvis, Yuri Volkov, Dania Movia
Alternative models such as three-dimensional (3D) cell cultures represent a distinct milestone towards capturing the realities of cancer biology in vitro and reduce animal experimentation in the preclinical stage of drug discovery. Significant work remains to be done to understand how substrates used in in vitro alternatives influence cancer cells phenotype and drug efficacy responses, so that to accurately link such models to specific in vivo disease scenarios. Our study describes how the morphological, mechanical and biochemical properties of adenocarcinoma (A549) cells change in response to a 3D environment and varying substrates...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429513/protective-roles-of-rutin-against-restraint-stress-on-spermatogenesis-in-testes-of-adult-mice
#12
Asif Mehfooz, Quanwei Wei, Kaizhi Zheng, Mohamed Babo Fadlalla, Gracijela Maltasic, Fangxiong Shi
In order to investigate the effects of Rutin against restraint stress, 50 adult male mice were divided into five groups: control, restraint stress (RS), and RS with 2 doses of Rutin treatments. Mice were restrained in a conical tube for 4 h daily and Rutin was injected intraperitoneally for 15 consecutive days. Restraint stress significantly decreases body weights, testis and epididymis weights, thymus weights, visceral fats, serum concentrations of testosterone, sperm counts, sperm motility and sperm viability, while it increases serum epinephrine levels, adrenal gland weights and abnormal sperms...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429512/effect-of-human-umbilical-cord-blood-derived-cd34-hematopoietic-stem-cell-on-the-expression-of-wnt4-and-p53-genes-in-a-rat-model-of-hepatocellular-carcinoma
#13
Rania Naiem Sherif, Hussein Abdellatif, Noha Hazem, Neven A Ebrahim, Dalia Saleh, Gamal Shiha, Huda Eltahry, Kamal G Botros, Omar M Gabr
BACKGROUND AND AIM OF THE WORK: Hepatocellular carcinoma (HCC) is the most frequent primary liver malignancy. Chronic liver injuries as chronic hepatitis C and hepatitis B viruses, aflatoxins consumption and nonalcoholic fatty liver disease are well-established causes of HCC. HCC is associated with a series of molecular changes, as alternation in glypican-3, P53 expression and Wnt/β-catenin pathway. Hepatic cancer progenitor cells could contribute to HCC development. This research aimed to study the effectiveness of human CD34+ hematopoietic stem cell on Wnt4 and P53 genes expression, histopathological grading and hepatic progenitor cells percentage in HCC rat model...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429511/hypoxia-perturbs-endothelium-by-re-organizing-cellular-actin-architecture-nitric-oxide-offers-limited-protection
#14
Akila Swaminathan, Dharanibalan Kasiviswanathan, Uma Maheswari Balaguru, Gopi Krishna Kolluru, Geetha SuryaKumar, Suvro Chatterjee
Exposure to hypoxia causes structural changes in the endothelial cell (EC) monolayer that alter its permeability. There was a report earlier of impairment of nitric oxide (NO) production in endothelium. The intervention of NO in the altered cellular arrangements of actin cytoskeleton in endothelium for rectification of paracellular gaps in endothelium under hypoxia was observed. The present study demonstrates hypoxia inducing paracellular gaps in hypoxia-exposed blood capillaries in chick embryo extravascular model...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429510/characterization-and-spatial-relationships-of-the-hepatic-vascular-biliary-tracts-and-their-associated-pancreocytes-and-macrophages-in-the-model-fish-guppy-poecilia-reticulata-a-study-of-serial-sections-by-light-microscopy
#15
Sisandra Sousa, Maria J Rocha, Eduardo Rocha
The guppy is a tropical fish that has been used as an experimental model organism in science. It is a species well adapted to the natural environment and that can support adverse environmental conditions, and so, at occasions, its presence can be indicative of environmental disturbances. Moreover, as the liver is very important when studying fish diseases, the knowledge of normal microanatomy is essential to assess histological changes, e.g., related to environmental change or toxic pollutants. The target organ of this histological study is the liver...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429509/physical-stimulation-and-scaffold-composition-efficiently-support-osteogenic-differentiation-of-mesenchymal-stem-cells
#16
Sepide Heydari Asl, Hoorieh Hosseinpoor, Kazem Parivar, Nasim Hayati Roodbari, Hana Hanaee-Ahvaz
BACKGROUND: Despite significant achievements in the field of tissue engineering, simplification and improvement of the existing protocols are of great importance. The use of complex differentiation media, due to the presence of multiple factors, may have some undesired effects on cell health and functions. Thus, minimizing the number of involved factors, while maintaining the differentiation efficiency, provides less costly and controllable conditions. Adipose-derived Mesenchymal stem cells (ASCs), the adult stem cells present in adipose tissue, can be a suitable source of stem cells due to abundant and ease of access...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29061447/pdk1-promotes-apoptosis-of-chondrocytes-via-modulating-mapk-pathway-in-osteoarthritis
#17
Qingtian Ge, Hailin Wang, Xinbao Xu, Libin Xu, Leilei Zhai, Ran Tao
3-Phosphoinositide dependent protein kinase-1 (PDK1), a serine threonine kinase, belongs to the AGC kinase family and is associated with apoptosis. The aim of this study was to investigate the expression of PDK1 (3-Phosphoinositide dependent protein kinase-1) in articular cartilage with osteoarthritis (OA) and to analyze the relationship between PDK1 and chondrocyte apoptosis. Immunohistochemistry and RT-PCR analysis showed that the expression of PDK1 in articular cartilage of OA patients and healthy controls...
December 2017: Tissue & Cell
https://www.readbyqxmd.com/read/29122297/cytochemical-identification-of-turbot-myeloperoxidase-positive-granulocytes-by-potassium-iodide-and-oxidized-pyronine-y-staining
#18
Heng Chi, Li-Lian Wen, Zhi-Hai Sui, Qing-Lei Sun, Li Sun
Cytomorphological and cytochemical staining are important methods for the identification of cell types, in particular in fish which often lack biological tools such as specific antibodies. Myeloperoxidase (MPO) is usually used as an intracellular marker of neutrophil accumulation in tissues and a marker of neutrophil activity in plasma. In this study, we reported a potassium iodide and oxidized pyronine Y (KI-PyY) staining method for rapid and highly sensitive detection of MPO-positive cells in turbot blood, peritoneum, and tissues...
November 3, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/29162289/apelin-apj-system-is-responsible-for-stress-induced-increase-in-atrial-natriuretic-peptide-expression-in-rat-heart
#19
Vecihe Nimet Izgut-Uysal, Nuray Acar, Ilknur Birsen, Filiz Ozcan, Ozlem Ozbey, Hakan Soylu, Sema Avci, Filiz Tepekoy, Gokhan Akkoyunlu, Gultekin Yucel, Ismail Ustunel
BACKGROUND: The cardiovascular system is a primary target of stress and stress is the most important etiologic factor in cardiovascular diseases. Stressors increase expressions of atrial natriuretic peptide (ANP) and apelin in cardiac tissue. AIM: The aim of the present study was to investigate whether stress-induced apelin has an effect on the expression of ANP in the right atrium of rat heart. METHODS: The rats were divided into the control, stress and F13A+stress groups...
October 31, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/29089090/transdifferentiation-of-mucous-neck-cells-into-chief-cells-in-fundic-gastric-glands-shown-by-gna-lectin-histochemistry
#20
Laura Gómez-Santos, Edurne Alonso, Lucio Díaz-Flores, Juan Francisco Madrid, Francisco José Sáez
The epithelium of the gastric mucosa and its glands in the corpus of rat stomach contains mucous surface cells (MSCs), parietal cells, mucous neck cells (MNCs), zymogenic or chief cells (ZCs), several types of enteroendocrine cells, and intermediate cells with characteristics between MNCs and ZCs also called transitional or prezymogenic cells (pre-ZCs). The aim of our work was to analyze the expression of Mannose (Man) in the rat gastric glands by means of Galanthus nivalis lectin (GNA) histochemistry to identify the differences between MNC, pre-ZCs and ZCs and to establish the relationships between these cells...
October 28, 2017: Tissue & Cell
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