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Tissue & Cell

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https://www.readbyqxmd.com/read/28302318/regional-differences-of-tibial-and-femoral-cartilage-in-the-chondrocyte-gene-expression-immunhistochemistry-and-composite-in-different-stages-of-osteoarthritis
#1
A Lahm, D Dabravolski, H Spank, H Merk, J Esser, R Kasch
The function of articular cartilage as an avascular tissue is mainly served by collagen type II and proteoglycan molecules. Within this matrix homeostasis between production and breakdown of the matrix is exceptionally sensitive. The current study was conducted to identify regional differences in specific alterations in cartilage composition during the osteoarthritic process of the human knee joint. Therefor the changes in the expression of the key molecules of the extracellular matrix were measured in dependence of the anatomical side (femoral vs tibial) and associated with immunohistochemistry and quantitative measurement...
February 24, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28279494/sustaining-immunity-during-starvation-in-bivalve-mollusc-a-costly-affair
#2
Elizabeth Mahapatra, Dishari Dasgupta, Navodipa Bhattacharya, Suvrotoa Mitra, Debakana Banerjee, Soumita Goswami, Nabanita Ghosh, Avijit Dey, Sudipta Chakraborty
Complete or partial depletion of resource in a freshwater habitat is a common phenomenon. As a consequence, aquatic fauna including bivalve molluscs may be exposed to dietary stress on a seasonal basis. Haemocyte based innate immune profile of the freshwater mollusc Lamellidens marginalis (Bivalvia: Eulamellibranchiata) was evaluated under starvation induced stress for a maximum period of 32 days in a controlled laboratory condition. During starvation, the bivalve haemocytes maintained a homeostasis in phagocytic efficacy and nitric oxide generation ability with respect to the control...
February 21, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28242104/structural-features-of-the-spermatozoon-of-a-passeridan-bird-the-carib-grackle-quiscalus-lugubris
#3
Tom A Aire, Lizette du Plessis, Mahesh S Deokar, Eugene Rennie, Sunil K Gupta
The spermatozoon of the Carib grackle, Quiscalus lugubris, a member of the family Icteridae, is generally similar in organization to the passerine-type of spermatozoon, in being highly elongated and displaying a helical structure of the acrosome, nucleus and principal piece of the tail. There are subtle variations in acrosomal structural features between this organelle in the grackle and that in some of the very few passerine species of birds in which the spermatozoon has been studied. The proximal centriole is present, and, thus, the Carib grackle is the third passeridan bird in which this organelle, hitherto regarded as absent in passerine birds, has been described in the spermatozoon...
February 21, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28242105/an-immunohistochemical-study-on-the-distribution-of-vasotocin-neurons-in-the-brain-of-two-weakly-electric-fish-gymnotus-omarorum-and-brachyhypopomus-gauderio
#4
Paula Pouso, Milka Radmilovich, Ana Silva
Hypothalamic nonapeptides (arginin vasotocin-vasopressin, oxytocin-isotocin) are known to modulate social behaviors across vertebrates. The neuroanatomical conservation of nonapeptide systems enables the use of novel vertebrate model species to identify general strategies of their functional mechanisms. We present a detailed immunohistochemical description of vasotocin (AVT) cell populations and their projections in two species of weakly electric fish with different social structure, Gymnotus omarorum and Brachyhypopomus gauderio...
February 16, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28222889/pre-cancer-risk-assessment-in-habitual-smokers-from-dic-images-of-oral-exfoliative-cells-using-active-contour-and-svm-analysis
#5
Susmita Dey, Ripon Sarkar, Kabita Chatterjee, Pallab Datta, Ananya Barui, Santi P Maity
Habitual smokers are known to be at higher risk for developing oral cancer, which is increasing at an alarming rate globally. Conventionally, oral cancer is associated with high mortality rates, although recent reports show the improved survival outcomes by early diagnosis of disease. An effective prediction system which will enable to identify the probability of cancer development amongst the habitual smokers, is thus expected to benefit sizable number of populations. Present work describes a non-invasive, integrated method for early detection of cellular abnormalities based on analysis of different cyto-morphological features of exfoliative oral epithelial cells...
February 9, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28222887/rare-er-protein-misfolding-mistrafficking-disorders-therapeutic-developments
#6
REVIEW
Ramanath Narayana Hegde, Advait Subramanian, Prathyush Pothukuchi, Seetharaman Parashuraman, Alberto Luini
The presence of a functional protein at the appropriate location in the cell is the result of the processes of transcription, translation, folding and trafficking to the correct destination. There are numerous diseases that are caused by protein misfolding, mainly due to mutations in the respective gene. The consequences of this misfolding may be that proteins effectively lose their function, either by being removed by the cellular quality control machinery or by accumulating at the incorrect intracellular or extracellular location...
February 9, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28222888/acinar-cell-response-to-liquid-diet-during-rats-growth-period-differs-in-submandibular-and-sublingual-glands-from-that-in-parotid-glands
#7
Shigeru Takahashi, Hiroki Uekita, Hiroto Taniwaki, Takanori Domon
Continuously feeding a liquid diet to growing rodents strongly inhibits parotid gland growth, due to suppressed growth of acinar cells. This study investigated whether a liquid diet had a similar effect on submandibular and sublingual glands of growing rats. Rats were weaned on day 21 after birth and then fed a pellet diet in the control group and a liquid diet in the experimental group for 0, 1, 2, 4, and 8 weeks. Their submandibular and sublingual glands were excised, weighed, and examined histologically, immunohistochemically (using antibodies to 5'-bromo-2-deoxyuridine and cleaved caspase 3), and ultrastructurally...
February 8, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28238347/liver-and-kidney-toxicity-induced-by-afzal-smokeless-tobacco-product-in-oman
#8
Nawal Al-Mukhaini, Taher Ba-Omar, Elsadig Eltayeb, Aisha Al-Shihi, Nafila Al-Riyami, Jamila Al-Belushi, Kawthar Al-Adawi
Afzal, the common smokeless tobacco product (STP) in Oman, is believed to contain toxins that may impair the function of some organs such as liver and kidney. An aqueous extract from Afzal was added to drinking water to be administrated orally to Wistar albino rats (n=72) young and adult from both genders weighing between 60-80g and 150-240g respectively for 8 weeks. Animals were divided into three groups: control (distilled water instead of Afzal extract), low-dose (3mgnicotine/kgbodyweight/day) and high-dose (6mgnicotine/kgbodyweight/day)...
February 6, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28190551/proliferation-of-canine-bone-marrow-derived-mesenchymal-stem-cells-on-different-nanomaterial-based-thin-film-scaffolds
#9
Kinsuk Das, Bhabesh Mili, Madhusoodan A P, Abhishek Chandra Saxena, Ajay Kumar, Praveen Singh, Med Ram Verma, Mihir Sarkar, Sadhan Bag
Stem cell niche research uses nanotechnologies to mimic the extra-cellular microenvironment to promote proliferation and differentiation. The aim of designing different scaffolds is to simulate the best structural and environmental pattern for extracellular matrix. This experiment was designed to study the proliferative behaviour of canine bone marrow deriver mesenchymal stem cells (MSCs) on different nanomaterial based thin film scaffolds of carbon nanotubes (CNT), chitosan and poly ε-caprolactone. Similar number of cells was seeded on the scaffolds and standard cell culture flask, taken as control...
February 6, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28237322/effect-of-chronic-administration-of-sildenafil-citrate-viagra-on-the-histology-of-the-retina-and-optic-nerve-of-adult-male-rat
#10
Sohair A Eltony, Sally Y Abdelhameed
BACKGROUND: Abnormal vision has been reported by 3% of patients treated with sildenafil citrate (Viagra). Although many men use Viagra for an extended period for treatment of erectile dysfunction, the implications of the long term-daily use of it on the retina and optic nerve are unclear. AIM OF THE WORK: To investigate the effect of chronic daily use of sildenafil citrate in a dose equivalent to men preferred therapeutic dose on the histology of the retina and optic nerve of adult male rat...
February 5, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28320513/morphology-and-ultrastructure-of-the-adrenal-gland-in-bactrian-camels-camelus-bactrianus
#11
Wen-Ling Ye, Feng-Ling Wang, Hong-Ju Wang, Jian-Lin Wang
In the present study, we examined the morphological features of the adrenal gland in Bactrian camel by means of digital anatomy, light and electron microscopy. Our findings testified that the gland was divided into three parts, capsule, cortex and medulla from outside to inside as other mammals, and the cortex itself was further distinguished into four zones: zona glomerulosa, zona intermedia, zona fasciculate and zona reticularis. Notably, the zona intermedia could be seen clearly in the glands from females and castrated males, whereas it was not morphologically clear in male...
January 31, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28209368/correlation-of-micro-vessel-density-and-c-myc-expression-in-breast-tumor-of-mice-following-mesenchymal-stem-cell-therapy
#12
Maryam Adelipour, Fatemeh Babaei, Mohammadreza Mirzababaei, Abdolamir Allameh
Stem cell therapy for degenerative diseases has been established; however there are controversies over the treatment of solid tumors with stem cell transplantation. In the present study, the anti-tumor action of mesenchymal stem cells (MSCs) has been examined in a mouse model of breast cancer with emphasize on tumor growth, angiogenesis and c-Myc expression in breast tumors. For this purpose, MSCs were isolated from bone marrow of Balb/c mice and characterized. A Balb/c mouse model of breast cancer was developed and subjected to cell therapy intra venous (I...
January 30, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28187870/the-unique-organization-of-filamentous-actin-in-the-medullary-canal-of-the-medulla-oblongata
#13
Bai-Hong Tan, Chun-Yan Guo, Tian-Qing Xiong, Ling-Meng Chen, Yan-Chao Li
In the central canal, F-actin is predominantly localized in the apical region, forming a ring-like structure around the circumference of the lumen. However, an exception is found in the medulla oblongata, where the apical F-actin becomes interrupted in the ventral aspect of the canal. To clarify the precise localization of F-actin, the fluorescence signals for F-actin were converted to the peroxidase/DAB reaction products in this study by a phalloidin-based ultrastructural technique, which demonstrated that F-actin is located mainly in the microvilli and terminal webs in the ependymocytes...
January 24, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28256256/therapeutic-potentials-of-mesenchymal-stem-cells-on-the-renal-cortex-of-experimentally-induced-hypertensive-albino-rats-relevant-role-of-nrf2
#14
Eman M Mohamed, Mai A Samak
Bone marrow derived-mesenchymal stem cells (BM-MSCs) have brought great attention in regenerative medicine field, various experimental & clinical trials were held to investigate their therapeutic effects in different disorders. We designed a histological & immunohistochemical study to evaluate effectiveness of MSCs therapy in withhold of end-stage renal disease (ESRD) secondary to hypertension which has become a growing & striking public health problem. 30 adult male albino rats were utilized, 20 of them were exposed to experimental induction of hypertension, then divided equally to MSCs treated group (injected with 1×10(6) fluorescent labeled cell i...
January 20, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28162243/seasonal-morphophysiological-variations-in-the-prostatic-complex-of-the-tarabul-s-gerbil-gerbillus-tarabuli
#15
Arezki Kheddache, Elara N'tima Moudilou, Yamina Zatra, Naouel Aknoun-Sail, Zaina Amirat, Jean-Marie Exbrayat, Farida Khammar
Gerbillus tarabuli is a nocturnal Saharan rodent which has an annual reproductive cycle characterized by the reproductive activity in spring and a long phase of sexual quiescence in other seasons. We describe the morphology and hormonal regulation of the prostatic complex of this rodent in the two periods, based on anatomical, histological, morphometric, and immunohistochemical analyses. The organisation of this prostatic complex is similar to that reported for Meriones unguiculatus, but different from the prostate of Psammomys obesus, the rat, and the mouse...
January 19, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28109546/influence-of-feeding-habits-in-the-endocrine-pancreas-of-insectivore-bat-pteronotus-personatus-and-nectarivore-bat-anoura-geoffroyi-a-comparative-stereological-and-immunohistochemical-study
#16
Clarice Machado-Santos, Júlio Cesar Fraulob Aquino, Patrício Adriano da Rocha, Marcelo Abidu-Figueiredo, Lycia de Brito-Gitirana, Armando Sales
Pteronotus personatus as an insectivore bat and has a diet that consists of a high protein diet, whereas the diet of Anoura geoffroyi, a predominantly nectarivore bat, is rich in simple sugars like sucrose, glucose and fructose. Considering that diet influences the activation of different pathways, which may influence morphological adaptations in the gastrointestinal system, the aim of this study was to compare the morphology of the endocrine pancreas in P. personatus and A. geoffroyi. For this, histological, stereological and immunohistochemical methods were used...
January 16, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28087142/ultrastructural-characteristics-of-the-mature-spermatozoon-of-the-digenean-sclerodistomum-italicum-stossich-1893-hemiuroidea-sclerodistomidae-intestinal-parasite-of-hypocanthus-amia-teleostei-carangidae
#17
Papa Ibnou Ndiaye, Yann Quilichini, Adji Mama Marigo, Cheikh Tidiane Bâ, Vasyl V Tkach, Bernard Marchand
The mature spermatozoon of Sclerodistomum italicum is filiform, tapered at both ends and shows the following features: 2 axonemes of the 9+"1" pattern of the Trepaxonemata, mitochondrion, nucleus and parallel cortical microtubules. The specific features of the spermatozoon of S. italicum include the simultaneous presence of two types of extramembranous ornamentations, the presence of short cortical microtubules in the anterior part of the spermatozoon and the presence of only one bundle of cortical microtubules in the median part of the spermatozoon...
December 30, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28073590/objective-detection-of-apoptosis-in-rat-renal-tissue-sections-using-light-microscopy-and-free-image-analysis-software-with-subsequent-machine-learning-detection-of-apoptosis-in-renal-tissue
#18
Nayana Damiani Macedo, Aline Rodrigues Buzin, Isabela Bastos Binotti Abreu de Araujo, Breno Valentim Nogueira, Tadeu Uggere de Andrade, Denise Coutinho Endringer, Dominik Lenz
OBJECTIVE: The current study proposes an automated machine learning approach for the quantification of cells in cell death pathways according to DNA fragmentation. METHODS: A total of 17 images of kidney histological slide samples from male Wistar rats were used. The slides were photographed using an Axio Zeiss Vert.A1 microscope with a 40x objective lens coupled with an Axio Cam MRC Zeiss camera and Zen 2012 software. The images were analyzed using CellProfiler (version 2...
December 28, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28088324/protective-effect-of-sugar-cane-extract-against-dextran-sulfate-sodium-induced-colonic-inflammation-in-mice
#19
Bin Wang, Yansen Li, Masami Mizu, Toma Furuta, ChunMei Li
Sugar cane extract (SCE) exhibits various biological effects and has been reported to enhance animal growth performance. However, the effect of SCE on inflammation in animals is still obscure. To study the effects and underlying mechanism of SCE on dextran sulfate sodium (DSS)-induced colonic inflammation, forty female ICR mice (26.63±0.19g, 6-week-old) were assigned into four groups: a control group (Cont), a DSS-challenged group (DSS), a SCE-supplemented group (SCE), and a DSS+SCE group (DSS+SCE). Mice in Cont group and DSS group were fed basic diet and other mice received 1% SCE supplemented in basic diet from 6-week to 8-week-old...
December 26, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28049551/beta-catenin-can-induce-hair-follicle-stem-cell-differentiation-into-transit-amplifying-cells-through-c-myc-activation
#20
Qiong Shen, Weirong Yu, Yong Fang, Min Yao, Penggao Yang
Hair follicle stem cells play important roles in maintaining homeostasis and skin tissue self-renewal. Transit-amplifying cells represent the transition of cells from hair follicle stem cells into differentiated epidermal cells. Thus far, the signaling pathway and the molecular biological mechanism that regulate the proliferation and differentiation of hair follicle stem cells remain unclear. In this paper, we studied the relationship between β-catenin and c-myc during the process of the differentiation of hair follicle stem cells into transit-amplifying cells...
December 21, 2016: Tissue & Cell
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