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Tissue & Cell

T Martinovic, D Ciric, I Pantic, K Lalic, I Rasulic, S Despotovic, I Lalic, D Djuricic, V Bumbasirevic, T Kravic-Stevovic
Type 2 diabetes is an endocrine disorder characterized with hyperglycemia, hyperinsulinemia and insulin resistance. Morphological changes in cell nuclei in diabetes were recently detected. The aim of this study was to compare electron microscopic features of lymphocyte nuclei in type 2 diabetes and healthy individuals using conventional computer assisted methods, fractal analysis and gray level co-occurrence matrix (GLCM) analysis of nuclear chromatin. Mononuclear cells taken from the peripheral blood of newly diagnosed type 2 diabetes patients, metformin treated type 2 diabetes patients and healthy individuals were analyzed with transmission electron microscope...
June 2018: Tissue & Cell
Seong Yeong An, Jung Sun Heo
This study examined the effects of low oxygen tension on the osteogenic differentiation of embryonic stem cells (ESCs) in a three-dimensional culture system. The high expression levels of hypoxia-related proteins hypoxia-inducible factor-1α and vascular endothelial growth factor were first validated in ESCs subjected to hypoxic conditions compared with normoxic controls. The osteogenic differentiation of hypoxic ESCs with either osteogenic or osteogenic factor-free media was subsequently evaluated by measuring alkaline phosphatase activity, intracellular calcium levels, matrix mineralization, and the protein levels of osteogenic markers Runt-related transcription factor 2 and osterix...
June 2018: Tissue & Cell
Malgorzata Kotula-Balak, Agnieszka Milon, Piotr Pawlicki, Malgorzata Opydo-Chanek, Anna Pacwa, Klaudia Lesniak, Malgorzata Sekula, Marta Zarzycka, Monika Bubka, Waclaw Tworzydlo, Barbara Bilinska, Anna Hejmej
In this study, we demonstrate, for the first time, estrogen-related receptor (ERR) regulation of the physiological and biochemical status of testicular tumor Leydig cells. In a mouse tumor Leydig cells, ERRs (α, β, and γ) were silenced via siRNA. Cell morphology and cell physiology (proliferation and observation of monolayer formation) were performed by inverted phase-contrast microscope. Leydig cell functional markers (steroid receptors and signaling molecules) were examined by immunofluorescence and Western blotting...
June 2018: Tissue & Cell
Thafar Almela, Sarmad Al-Sahaf, Ian M Brook, Kimia Khoshroo, Morteza Rasoulianboroujeni, Farahnaz Fahimipour, Mohammadreza Tahriri, Erfan Dashtimoghadam, Robert Bolt, Lobat Tayebi, Keyvan Moharamzadeh
Recent advances in three-dimensional printing technology have led to a rapid expansion of its applications in tissue engineering. The present study was designed to develop and characterize an in vitro multi-layered human alveolar bone, based on a 3D printed scaffold, combined with tissue engineered oral mucosal model. The objective was to incorporate oral squamous cell carcinoma (OSCC) cell line spheroids to the 3D model at different anatomical levels to represent different stages of oral cancer. Histological evaluation of the 3D tissue model revealed a tri-layered structure consisting of distinct epithelial, connective tissue, and bone layers; replicating normal oral tissue architecture...
June 2018: Tissue & Cell
Ramesh Periasamy, Daniel V Surbek, Andreina Schoeberlein
The loss of oligodendrocyte progenitor cells (OPC) is a hallmark of perinatal brain injury. Our aim was to develop an in vitro culture condition for human chorion-derived mesenchymal stem cells (MSC) that enhances their stem cell properties and their capability to differentiate towards OPC-like cells. MSC were grown either in serum replacement medium (SRM) or serum-containing medium (SM) and tested for their morphology, proliferation, secretome, migration, protein expression and differentiation into OPC-like cells...
June 2018: Tissue & Cell
Satish Kumar Ramraj, Katie M Smith, Naveena B Janakiram, Coralee Toal, Ankita Raman, Doris Mangiaracina Benbrook
Human fallopian tube fimbria secretory epithelial cells (hFTSECs) are considered an origin of ovarian cancer and methods for their culture from fallopian tube specimens have been reported. Our objective was to determine whether characteristics of the donors or surgeries were associated with the capacities of fimbria specimens to generate hFTSEC cultures or their immune profiles. There were no surgical complications attributable to fallopian tube removal. Attempts to establish primary hFTSEC cultures were successful in 37 of 55 specimens (67%)...
June 2018: Tissue & Cell
Aline Freitas de Paula Melo, Monique Culturato Padilha Mendonça, Raquel de Mendonça Rosa-Castro
Kefir is a probiotic fermented milk product produced from grains with a complex composition of bacteria and yeasts that live in a symbiotic association. Anti-proliferative, anti-inflammatory, and anti-mutagenic effects are some of the health beneficial properties of kefir grains. The present study was conducted to evaluate whether regular consumption of kefir milk would be capable of preventing the development of pre-neoplastic lesions induced by azoxymethane (AOM). Aberrant crypt foci were induced in BALB-c mice via 2 subcutaneous injections of azoxymethane (15 mg/kg) and kefir was administered by daily gavage for 8 weeks (5 ml/kg)...
June 2018: Tissue & Cell
Anne-Eva van der Wijk, Ilse M C Vogels, Henk A van Veen, Cornelis J F van Noorden, Reinier O Schlingemann, Ingeborg Klaassen
The inner blood-retinal barrier (BRB) is made up by the neurovascular unit, consisting of endothelial cells, pericytes and glial cells. The BRB maintains homeostasis of the neural retina, but in pathological eye conditions the neurovascular unit is often disrupted, causing BRB loss. Here, we investigated in detail temporal and spatial recruitment of the neurovascular unit in the neonatal mouse retina from postnatal day (P)3 to P25 employing immunohistochemical staining of vascular endothelium (isolectin B4), pericytes (α-SMA and NG2) and astrocytes (GFAP)...
June 2018: Tissue & Cell
Inci Turan, Hale Sayan Ozacmak, V Haktan Ozacmak, Figen Barut, I Diler Ozacmak
BACKGROUND: Intestinal ischemia and reperfusion (I/R) induces oxidative stress, inflammatory response, and acute lung injury. S-nitrosoglutathione (GSNO), a nitric oxide donor, has been documented to have protective effects on experimental ischemia models. AIM: The aim of this study was to examine the effect of GSNO on I/R-induced intestine and lung damage and detect the potential mechanisms emphasizing the protective role of GSNO. METHODS: Intestinal I/R was induced by occluding the superior mesenteric artery for 30 min followed by reperfusion for 180 min...
June 2018: Tissue & Cell
J Y Jiang, Y Y Pan, Y Cui, J F Fan, Q Li, S J Yu
We isolated and purified yak (Bos grunniens) endometrial epithelial cells and assayed different concentrations of estradiol (E2 ) and progesterone (P4 ) with respect to secretion of epidermal growth factor (EGF) and insulin-like growth factor-1 (IGF-1). Uterine epithelia were confirmed with Feulgen staining, karyotype analysis, and immunohistochemistry. Then, cells were treated with E2 and P4 and cultured in serum-free medium for 24 h. EGF and IGF-1 were measured with immunofluorescence, Real-time PCR (RT-PCR), Western blot (WB), and ELISA...
June 2018: Tissue & Cell
Lara Paro Dias, Ângela C Malheiros Luzo, Bruno B Volpe, Marcela Durán, Sofia E M Galdames, Luiz A B Ferreira, Nelson Durán, Wagner J Fávaro
This study describes the effects of a promising therapeutic alternative for non-muscle invasive bladder cancer (NMIBC) based on Bacillus Calmette-Guerin (BCG) intravesical immunotherapy combined with Platelet-rich plasma (PRP) in an animal model. Furthermore, this study describes the possible mechanisms of this therapeutic combination involving Toll-like Receptors (TLRs) 2 and 4 signaling pathways. NMIBC was induced by treating female Fischer 344 rats with N-methyl-N-nitrosourea (MNU). After treatment with MNU, the animals were distributed into four experimental groups: Control (without MNU) group, MNU (cancer) group, MNU + PRP group, MNU + BCG group and MNU + PRP + BCG group...
June 2018: Tissue & Cell
Rosa Jahangiri, Fatemeh Mosaffa, Masoumeh Gharib, Amir Nader Emami Razavi, Afshin Abdirad, Khadijeh Jamialahmadi
PAX2 (paired box gene 2) is a transcription factor, which is involved in both cell proliferation and carcinogenesis. This study aimed to investigate PAX2 expression in tamoxifen resistant (TAM-R) and tamoxifen sensitive (TAM-S) breast carcinoma patients and analyze its correlation with clinicopathological characteristics and survival. Immunohistochemical analysis was performed to evaluate PAX2 protein expression in 36 TAM-R and 36 TAM-S formalin-fixed paraffin-embedded (FFPE) breast tumor tissues. Data analysis indicated that PAX2 expression was significantly higher in TAM-S group in comparison to TAM-R (P = 0...
June 2018: Tissue & Cell
Kiran Yellappa Vajanthri, Parul Yadav, Suruchi Poddar, Sanjeev Kumar Mahto
Optogenetics is a new and emerging field that involves techniques of optics and genetic engineering to influence cellular functionality. In this work, we have successfully incorporated a non-selective cationic channel channelrhodopsin-2 (ChR2) into human hepatocellular carcinoma (HepG2) cells. A plasmid construct AAV-CAG-ChR2-GFP was used for liposomal transfection into the cells. ChR2 is a light sensitive membrane channel that opens upon illumination with blue light. Plasmid DNA isolation from E. coli XL 10 gold bacteria by alkaline lysis method resulted in a DNA concentration of 1150 μg/mL...
June 2018: Tissue & Cell
Kei Kawaguchi, Kentaro Igarashi, Kentaro Miyake, Thinzar M Lwin, Masuyo Miyake, Tasuku Kiyuna, Ho Kyoung Hwang, Takashi Murakami, Jonathan C Delong, Shree Ram Singh, Bryan Clary, Michael Bouvet, Michiaki Unno, Robert M Hoffman
Pancreatic cancer is resistant to treatment and needs precision individualized therapy to improve the outcome of this disease. Previously, we demonstrated that trametinib (TRA), a MEK inhibitor, could inhibit a pancreatic cancer patient-derived orthotopic xenograft (PDOX). In the present study, we show that gemcitabine (GEM) in combination with TRA was more effective than TRA alone. We implanted a patient pancreatic cancer orthotopically in the pancreatic tail of nude mice to establish the PDOX model. After seven weeks of tumor growth, we divided 32 pancreatic-cancer PDOX nude mice into 4 groups of eight: untreated control; GEM (once a week for 2 weeks); TRA (14 consecutive days); GEM + TRA (GEM: once a week for 2 weeks, TRA:14 consecutive days)...
June 2018: Tissue & Cell
Carlos Landa-Solís, Anell Olivos-Meza, Brenda Olivos-Díaz, Adriana Hernández-Rangel, Víctor Hugo Cárdenas-Soria, Maria Cristina Velasquillo-Martínez, José Clemente Ibarra-Ponce de León
Today, regenerative medicine requires new sources of multipotent stem cells for their differentiation to chondrocytes using the mediums of differentiation available in the market. This study aimed to determine whether the Mesenchymal Stem Cells (MSCs) isolated from Mobilized Peripheral Blood (MPB) in sheep using the Granulocyte Colony-Stimulating Factor (G-CSF), have the ability of first acquire a fibroblast-like morphology after being forced out of the bone marrow niche by G-CSF and second, if the cells have the capacity to express collagen type-II α I in primary culture using a human commercial media of differentiation...
June 2018: Tissue & Cell
Aliaa S A Al-Afify, Gehan El-Akabawy, Neveen M El-Sherif, Fatma El-Nabawya A El-Safty, Mostafa M El-Habiby
Osteoarthritis (OA), the most common type of arthritis, is a disabling progressive disease mainly affecting the elderly and is becoming a major public health problem. Current therapies for OA provide only palliative pain relief and therapeutic candidates that are able to slow the progression of structural deterioration is a major unmet need for this disorder. Avocado soybean unsaponifiables (ASU) has proven its safety and effectiveness in clinical studies of knee osteoarthritis (OA); however, whether ASU exerts structure-modifying effects is still to be elucidated...
June 2018: Tissue & Cell
Meda-Romana Simu, Emoke Pall, Teodora Radu, Maria Miclaus, Bogdan Culic, Anca-Stefania Mesaros, Alexandrina Muntean, Gabriela Adriana Filip
In this study we designed a composite biomaterial based on a high viscosity soft propolis extract (70% propolis) and shell clam, with antiseptic and osteoinductive qualities, that can be used in dentistry, orthopedics and other areas where hard tissue regeneration is needed. We assessed it in interaction with stabilized human cells isolated from dental papilla of wisdom teeth (D1MSCs). We performed detailed characterization of the obtained material by Scanning Electronic Microscopy (SEM), X-Ray Diffraction (XRD), Energy Dispersive X-Ray Spectroscopy (EDX), Fourier Transform Infrared Spectroscopy (FTIR) techniques...
June 2018: Tissue & Cell
Xiu-Hua Li, Shun-Jin Liu, Xiao-Yu Liu, Hai-Yu Zhao, Mao-Geng Yang, De-Xin Xu, Jing Guo, Jing-Hui Li, Juan-Juan Li
Sirtuin 3 (SIRT3) mediates cellular resistance toward various forms of stress. SIRT3 expression in the developing brain, especially its localization in various glial cell types, has not been fully explored. This study aimed to determine SIRT3 expression in the brain of neonatal rats subjected to hypoxia. By immunohistochemistry, immunofluorescence and Western blotting, we show here that SIRT3 expression in the periventricular white matter was up-regulated in hypoxia group compared with the control group at the corresponding time points...
June 2018: Tissue & Cell
Dalia Ibrahim
The glycoconjugates pattern of acidic secretions and distribution of chemosensory cells (SCCs) in the respiratory mucosa of dromedary camels were analyzed so as to identify their functional role. Secretions of the goblet cells and mucous glandular cells were analyzed to evaluate the variety of sugar chains, focusing on the acidic glycoconjugates. Using lectin histochemistry, WGA, STL, DBA, SBA, VVA and RCA-120 intensely bound to the goblet cells. PNA and ECL labeled the goblet cells with moderate intensity...
April 2018: Tissue & Cell
Fei Peng, Xianxian Chen, Ting Meng, En Li, Yongkang Zhou, Shengzhou Zhang
The Chinese alligator Alligator sinensis is an endangered freshwater crocodilian species endemic to China. Hematology and serum biochemistry reference range are useful in the assessment and management of animal health condition. In this study, a total of 74 Chinese Alligators (30 males and 44 females) were examined to establish reference range values of hematology and serum biochemistry parameters during the active and hibernating periods. We measured and analyzed 9 hematology and 21 serum biochemistry parameters including 4 serum electrolyte parameters, and described the morphology of different types of blood cells...
April 2018: Tissue & Cell
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