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Tissue & Cell

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https://www.readbyqxmd.com/read/28958480/protein-profiling-and-angiogenic-effect-of-hypoxia-cultured-human-umbilical-cord-blood-derived-mesenchymal-stem-cells-in-hindlimb-ischemia
#1
Kyu-Hyun Han, Ae-Kyeong Kim, Min-Hee Kim, Do-Hyung Kim, Ha-Nl Go, Donglim Kang, Jong Wook Chang, Soon Won Choi, Kyung-Sun Kang, Dong-Ik Kim
The aim of the present study was to investigate protein profiles of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) cultured in normoxic (21% O2) and hypoxic (1% O2) conditions, and evaluate oxygenation effects on angiogenesis in an ischemic hindlimb mouse model using a modified ischemic scoring system. Hypoxic conditions did not change the expression of phenotypic markers and increased adipogenesis and chondrogenesis. Epidermal growth factor (EGF), transforming growth factor alpha (TGF-α), TGF-β RII, and vascular endothelial growth factor (VEGF) were upregulated in the conditioned medium of hypoxic hUCB-MSCs, which are commonly related to angiogenesis and proliferation of biological processes by Gene Ontology...
September 20, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28947064/impact-of-pre-incubation-time-of-silk-fibroin-scaffolds-in-culture-medium-on-cell-proliferation-and-attachment
#2
Mehdi Amirikia, Seyed Mohammad Ali Shariatzadeh, Seyed Gholam Ali Jorsaraei, Malek Soleimani Mehranjani
Cell behaviours such as proliferation and attachment can be affected by the length of pre-incubation period of the scaffolds in the culture medium for long term. The aim of this study was to investigate the long term pre-incubation of 3D silk fibroin scaffolds in complete culture medium on cell attachment and proliferation. After the preparation of silk fibroin scaffolds by the technique of freeze drying, the scaffolds were pre-incubated in complete culture medium for 2 d, 6 d or 10 d before apical papilla stem cells (SCAP) seeding...
September 20, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28947065/enhanced-neuroprotective-effect-of-mild-hypothermia-with-vpa-against-ethanol-mediated-neuronal-injury
#3
Sandeep Kumar Vishwakarma, Avinash Bardia, L Chandrakala, Sana Arshiya, Syed Ameer Basha Paspala, Vishnupriya Satti, Aleem Ahmed Khan
INTRODUCTION: Progress in understanding pathophysiological mechanisms and the development of targeted regenerative strategies have been hampered by the lack of predictive disease models, specifically for the conditions to which affected cell types are inaccessible. The present study has aimed to unearth the role of valproic acid (VPA) and mild hypothermia (MH) as promising strategy to enhance the neuroprotective mechanisms in undifferentiated and differentiated human neural precursor cells (hNPCs) against ethanol-induced damage...
September 14, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28935358/ultrastructural-variation-tune-wing-coloration-of-a-moth-asota-caricae-fabricius-1775
#4
Monalisa Mishra, Nibedita Nayak, Harekrushna Sahoo
Butterflies and moths develop highly ordered coloration in their wing for signal transmission. We have investigated the ultrastructural arrangement of wing coloration of a moth Asota caricae, applying light, optical polarized, and scanning electron microscopy, and spectrophotometry. The forewing of the moth is brown in color with a white spot at the center. The hindwing is golden yellow in color with many black patches in it. The ventral part of the forewing and dorsal hindwing share the similar color pattern...
September 13, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28927747/astrocyte-elevated-gene-1-promotes-inflammation-and-invasion-of-fibroblast-like-synoviocytes-in-rheumatoid-arthritis
#5
Ruilong Hong, Kun Wang, Hongguang Shi
Astrocyte elevated gene-1 (AEG-1) was initially induced by HIV-1 infection and involved in tumor progression, migration and invasion as a nuclear factor-κB (NF-κB)-dependent gene. The present study we intended to investigate the protein expression of AEG-1 significantly associated with rheumatoid arthritis. Western blot analysis and immunohistochemistry demonstrated that AEG-1 was upregulated in synovial tissue of RA patients compared with the controls. Double immunofluorescent staining suggested that AEG-1 was expressed in fibroblast-like synoviocytes (FLS) of RA patients...
September 13, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28919010/germ-cell-proliferation-and-apoptosis-during-testicular-regression-in-a-seasonal-breeding-fish-kept-in-captivity
#6
Yves Moreira Ribeiro, Santer Alvares de Matos, Fabricio Flavio Theophilo Domingos, Helio Batista Dos Santos, Augusto Bicalho Cruz Vieira, Nilo Bazzoli, Elizete Rizzo
Cell proliferation and apoptosis regulate germ cells stock and sperm production, eliminate anomalous gametes, and are essential parameters to consider in fish farming. Herein, spermatogenic activity as well as germ cell proliferation and apoptosis were assessed in Leporinus taeniatus, a seasonal breeding species from the São Francisco River basin, Brazil. Testes of 24 adult fishes from a farming station were sampled between December and July and processed for light and transmission electron microscopy and immunohistochemistry for PCNA and TUNEL assay...
September 12, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28893403/energy-restriction-affect-liver-development-in-hu-sheep-ram-lambs-through-hippo-signaling-pathway
#7
Ting-Ting Zhang, Guo-Min Zhang, Yu-Hang Jin, Yi-Xuan Guo, Zhen Wang, Yi-Xuan Fan, M A El-Samahy, Feng Wang
This study aimed to evaluate the effects of dietary energy restriction on postnatal liver development in Hu sheep ram lambs. A total of 16 ram lambs were randomly divided into two groups: 100% energy requirements diet and 55% energy requirements diet, which were fed for 75 d. Results showed that the final body and liver weights decreased with energy restriction (p <0.05). Energy restriction caused a significant decrease in the levels of circulating insulin-like growth factor 1 (IGF1) and an increase in growth hormone secretion (p <0...
September 2, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28843337/up-regulation-of-traf2-suppresses-neuronal-apoptosis-after-rat-spinal-cord-injury
#8
Guanhua Xu, Jinlong Zhang, Lingling Wang, Zhiming Cui, Xu Sun, Zhen Liu, Zezhang Zhu, Yong Qiu
Tumor necrosis factor receptor-associated factor 2 (TRAF2) for signal transduction of the cell death receptor is well established. However, the role of TRAF2 in spinal cord injury (SCI) remains unclear. In this study, we detected the dynamic change patterns of TRAF2 expression using an acute spinal cord contusion (SCC) model in adult rats. Western blot analysis and immunohistochemistry identified significant upregulation of TRAF2 after SCI. Double-immunofluorescent staining demonstrated that the upregulated TRAF2 was found predominantly in neurons...
August 16, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28843336/the-effect-of-trimetazidine-and-diazoxide-on-immunomodulatory-activity-of-human-embryonic-stem-cell-derived-mesenchymal-stem-cell-secretome
#9
Saeed Jahandideh, Faezeh Maghsood, Nastaran Mohammadi Ghahhari, Majid Lotfinia, Mohammad Mohammadi, Behrooz Johari, Mehdi Kadivar
Comprehensive proteome profiling of the factors secreted by mesenchymal stem cells (MSCs), referred to as secretome, revealed that it consists of cytokines, chemokines, growth factors, extracellular matrix proteins, and components of regeneration, vascularization, and hematopoiesis pathways. Harnessing this MSC secretome for therapeutic applications requires the optimization of production of secretary molecules. A variety of preconditioning methods have been introduced, which subject cells to stimulatory molecules to create the preferred response and stimulate persistent effects...
August 16, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28823837/ultrastructure-of-the-larval-midgut-of-bittacus-planus-mecoptera-bittacidae-and-neopanorpa-longiprocessa-mecoptera-panorpidae
#10
Lu Liu, Bao-Zhen Hua
Bittacidae and Panorpidae are the two largest families in Mecoptera. The larvae of Bittacidae are different from those of Panorpidae in external morphology and habits, and have an interesting habit of spraying the body surface with soil through the anus. However, it remains unknown to date whether the larval midguts are different in structure between the two families. Here the ultrastructure of the larval midguts of the hangingfly Bittacus planus Cheng and the scorpionfly Neopanorpa longiprocessa Hua & Chou were compared using light, scanning, and transmission electron microscopy...
August 8, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28789814/colocalization-of-metastasis-associated-proteins-1-2-and-estrogen-receptor-alpha-in-rat-epididymis
#11
Pang Cheng, Jie Zhao, Huilian Qiao, He Ma, Binfang Ma, Jinhua Wei, Yunqing Li, Zhen Li
It has been suggested that metastasis-associated proteins 1 and 2 (MTA1 and MTA2) are capable of suppressing estrogen receptor alpha (ERα) transactivation activity in breast cancer cells. ERα, which is present in the epididymis, is a crucial mediator of maintaining the luminal environment necessary for proper sperm maturation and function. The present study was undertaken to analyze the expression profile of both MTA1 and MTA2 in the epididymis of rats and to ascertain whether MTA1/2 colocalizes with ERα in the epididymis and primary cultured epididymal epithelial cells...
July 29, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28780992/functional-morphology-of-the-olfactory-mucosa-and-olfactory-bulb-in-fossorial-rodents-the-east-african-root-rat-tachyoryctes-splendens-and-the-naked-mole-rat-heterocephalus-glaber
#12
P N Onyono, B M Kavoi, S G Kiama, A N Makanya
Optimal functioning of the olfactory system is critical for survival of fossorial rodents in their subterranean lifestyle. This study examines the structure of the olfactory mucosa and olfactory bulb of two fossorial rodents exhibiting distinct social behaviors, the East African root rat and the naked mole rat. The social naked mole rat displayed simpler ethmoturbinates consisting of dorsomedial and broad discoid/flaplike parts that projected rostrally from the ethmoid bone. In the solitary root rat however, the ethmoturbinates were highly complex and exhibited elaborate branching which greatly increased the olfactory surface area...
July 28, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28764928/tissue-engineering-dentin-pulp-complex-regeneration-approaches-a-review
#13
REVIEW
Batool Hashemi-Beni, Maryam Khoroushi, Mohammad Reza Foroughi, Saeed Karbasi, Abbas Ali Khademi
Dental pulp is a highly specialized tissue that preserves teeth. It is important to maintain the capabilities of dental pulp before a pulpectomy by creating a local restoration of the dentin-pulp complex from residual dental pulp. The articles identified were selected by two reviewers based on entry and exit criteria. All relevant articles indexed in PubMed, Springer, Science Direct, and Scopus with no limitations from 1961 to 2016 were searched. Factors investigated in the selected articles included the following key words: Dentin-Pulp Complex, Regeneration, Tissue Engineering, Scaffold, Stem Cell, and Growth Factors...
July 25, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28756875/glycopattern-analysis-of-acidic-secretion-in-the-intestine-of-the-red-eared-slender-turtle-trachemys-scripta-elegans-testudines-emydidae
#14
Giovanni Scillitani, Donatella Mentino, Maria Mastrodonato
The secretion of the goblet cells in the intestine of Trachemys scripta elegans was studied in situ by histochemical methods to analyze the diversity of sugar chains, with particular regard to the acidic glycans. Conventional histochemical stains (Periodic acid-Schiff, Alcian Blue pH 2.5, High Iron Diamine) and binding with ten FITC-labelled lectins combined with chemical and enzymatic pre-treatments were used to characterize the oligosaccharidic chains. The intestine can be divided into three regions, i.e...
July 23, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28851519/bioactive-glass-ceramic-nanoparticles-coated-poly-l-lactic-acid-scaffold-improved-osteogenic-differentiation-of-adipose-stem-cells-in-equine
#15
Fatemeh Sadat Mahdavi, Abdolreza Salehi, Ehsan Seyedjafari, Abdollah Mohammadi-Sangcheshmeh, Abdolreza Ardeshirylajimi
Horses with big bone fractures have low chance to live mainly due to the lake of a proper treatment strategy. We believe that further attempts in equine bone tissue engineering will probably be required to meet all the needs for the lesion therapies. Therefore in this study we aimed to investigate the osteogenic differentiation capacity of equine adipose-derived stem cells (e-ASCs) on nano-bioactive glass (nBGs) coated poly(l-lactic acid) (PLLA) nanofibers scaffold (nBG-PLLA). Using electrospinning technique, PLLA scaffold was prepared successfully and coated with nBGs...
July 20, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28844402/gastroprotective-and-mucosa-homeostatic-activities-of-coconut-milk-and-water-on-experimentally-induced-gastropathies-in-male-wistar-rats
#16
K O Ajeigbe, W M Owonikoko, V Egbe, I Iquere, G Adeleye
In this biphasic study, 45 male wistar rats were divided into 9 groups. In Phase 1, Group 1 was treated with normal saline and served as the overall control, group 2 was treated with 95% Ethanol and represents the ulcer control, groups 3 and 4 received coconut water (CW; 4ml/100g BWt) and milk (CM; 4ml/100g BWt) for 4weeks while group 5 received Omeprazole (Omep; 20mg/kg BWt) during terminal week. 95% Ethanol-induced ulceration followed the treatments in all except group 1. In the second phase, Group 1 was the overall control, group 2 served as ulcer control by receiving acetic acid only, group 3 received coconut milk, and group 4 received omep...
July 13, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28720305/ecto-5-nucleotidase-cd73-regulates-bone-formation-and-remodeling-during-intramembranous-bone-repair-in-aging-mice
#17
Vivian Bradaschia-Correa, Anne M Josephson, Alexander J Egol, Matthew M Mizrahi, Kevin Leclerc, Jason Huo, Bruce N Cronstein, Philipp Leucht
Ecto-5'-nucleotidase (CD73) generates adenosine, an osteoblast activator and key regulator of skeletal growth. It is unknown, however, if CD73 regulates osteogenic differentiation during fracture healing in adulthood, and in particular how CD73 activity regulates intramembranous bone repair in the elderly. Monocortical tibial defects were created in 46-52-week-old wild type (WT) and CD73 knock-out mice (CD73(-/-)) mice. Injury repair was analyzed at post-operative days 5, 7, 14 and 21 by micro-computed tomography (micro-CT), histomorphometry, proliferating cell nuclear antigen (PCNA) immunostaining, alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) histochemistry...
July 4, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28732559/socs-1-is-involved-in-tnf-%C3%AE-induced-mitochondrial-dysfunction-and-apoptosis-in-renal-tubular-epithelial-cells
#18
Chunyang Du, Fang Yao, Yunzhuo Ren, Yunxia Du, Jinying Wei, Haijiang Wu, Huijun Duan, Yonghong Shi
Tumor necrosis factor-α (TNF-α) is suggested to induce mitochondrial dysfunction and apoptosis of renal tubular epithelial cells that possibly exacerbates renal function in chronic kidney disease (CKD). Here we investigated whether suppressor of cytokine signaling-1 (SOCS-1), an inhibitor of cytokine signaling, was involved in TNF-α-induced human renal tubular epithelial cells (HKCs) oxidative stress and apoptosis. TNF-α promoted the protein and mRNA expression of SOCS-1 in a time and dose dependent manner, along with increased cell apoptosis and activation of apoptosis signal regulating kinase-1(ASK1) in HKCs...
June 30, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28669519/ex-vivo-models-of-the-retinal-pigment-epithelium-rpe-in-long-term-culture-faithfully-recapitulate-key-structural-and-physiological-features-of-native-rpe
#19
Savannah A Lynn, Gareth Ward, Eloise Keeling, Jenny A Scott, Angela J Cree, David A Johnston, Anton Page, Enrique Cuan-Urquizo, Atul Bhaskar, Martin C Grossel, David A Tumbarello, Tracey A Newman, Andrew J Lotery, J Arjuna Ratnayaka
The Retinal Pigment Epithelium (RPE) forms the primary site of pathology in several blinding retinopathies. RPE cultures are being continuously refined so that dynamic disease processes in this important monolayer can be faithfully studied outside the eye over longer periods. The RPE substrate, which mimics the supportive Bruch's membrane (BrM), plays a key role in determining how well in-vitro cultures recapitulate native RPE cells. Here, we evaluate how two different types of BrM substrates; (1) a commercially-available polyester transwell membrane, and (2) a novel electrospun scaffold developed in our laboratory, could support the generation of realistic RPE tissues in culture...
June 19, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28684045/effect-of-monocytes-macrophages-on-the-osteogenic-differentiation-of-adipose-derived-mesenchymal-stromal-cells-in-3d-co-culture-spheroids
#20
Hongbo Tang, Yang Zhang, John A Jansen, Jeroen J J P van den Beucken
This study aimed to investigate the distinctive roles of the monocytes and macrophages on osteogenic differentiation of adipose-derived mesenchymal stromal cells (ADMSCs) in 3D spheroid co-cultures. We hypothesized that monocytes or macrophages (subtypes pro-inflammatory M1 and pro-wound healing M2) would affect the osteogenic differentiation of ADMSCs in 3D spheroids and that cell-cell interactions between monocytes/macrophages and ADMSCs play an important role in the osteogenic differentiation process of ADMSCs...
June 15, 2017: Tissue & Cell
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