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Tissue & Cell

SangMin Kim, Yong-Seok Han, Jun Hee Lee, Sang Hun Lee
Mesenchymal stem cells (MSCs) are widely used in transplantation therapy due to their multilineage differentiation potential, abundance, and immuno-modulating ability. However, the risk of allograft rejection limits their application. Here, we proposed a novel method to facilitate MSC transplantation with enhanced applicability and efficacy. We cultured human adipose-derived MSCs in a 3D culture under in vitro expansion conditions and under conventional 2D adherent culture conditions. MSC spheroids promoted extracellular matrix molecules that stimulate MSC proliferation, and produced more angiogenic cytokines such as vascular endothelial growth factor, hepatocyte growth factor, and fibroblast growth factor than 2D-cultured MSCs...
August 2018: Tissue & Cell
Kaustav Chakraborty, Soumya Chatterjee, Arindam Bhattacharyya
Idiopathic pulmonary fibrosis is an irreversible, progressive and lethal lung disease. Regulatory T cells (Tregs) and Th17 cells both are involved in lung fibrosis. But there are only few reports regarding the effect of Treg on other T cell subsets in experimental lung fibrosis. The aim of this study was to investigate the impact of Treg on Th17, CD4+CD28-T, CD4+CD28+T and CD8 + T cell subsets that could drive lung fibrosis. To reach the goal of our study, first we depleted Tregs by anti-CD25 mAb injection in experimental C57BL/6 mice model...
August 2018: Tissue & Cell
Zhang Zhang, Zichen Wang, Wei Rui, Shiwei Wu
Multipotent neural stem cells could generate neurons and glial cells. Wide studies have been conducted to disclose the mechanism underlying neural stem cell differentiation and multiple factors have been identified in this field, one of which is bioactive components including natural herbal medicine. In this study, we found that magnesium lithospermate B is able to promote proliferation of neural stem cell in vitro. Besides, magnesium lithospermate B also induces generation of more neuronal cells and less glial cells...
August 2018: Tissue & Cell
Ya O Kamenev, M G Eliseikina, S I Maslennikov, I Yu Dolmatov
Fine structural features of Mizuhopecten yessoensis in different embryonic and larval developmental stages from oocyte to early veliger have been studied. Spermatozoa have a conical head, consisting of a short acrosome, nucleus, and 4 mitochondria in the middle piece. The flagellum is approximately 40 μm in length. Oocyte cytoplasm contains numerous yolk granules, which are gradually expended during the larval development and disappear at the early veliger stage. Gastrula has two invaginations: shell gland anlage on the dorsal side and ventral side archenteron...
August 2018: Tissue & Cell
N J Edwards, R Stone, R Christy, C K Zhang, B Pollok, X Cheng
Despite advances in tissue engineering and regenerative medicine, skin regeneration and cutaneous wound healing remains a significant medical challenge. A bioengineered skin that stimulates the body's natural regeneration capability is needed to address the current lack of treatment options. To this end, a biocompatible collagen wound matrix was developed using an electrochemical deposition fabrication process. The advanced collagen wound matrix has relatively high tensile strength compared to normal collagen matrix made by the heat gelation process and open porosity, and serves as an excellent platform for cellular growth and differentiation...
August 2018: Tissue & Cell
Yosuke Mukae, Manabu Itoh, Ryo Noguchi, Kojiro Furukawa, Ken-Ichi Arai, Jun-Ichi Oyama, Shuji Toda, Koichi Nakayama, Koichi Node, Shigeki Morita
BACKGROUND: We havebeen attempting to use cardiac spheroids to construct three-dimensional contractilestructures for failed hearts. Recent studies have reported that neuralprogenitors (NPs) play significant roles in heart regeneration. However, theeffect of NPs on the cardiac spheroid has not yet been elucidated. OBJECTIVE: This studyaims to demonstrate the influence of NPs on the function of cardiac spheroids. METHODS: Thespheroids were constructed on a low-attachment-well plate by mixing humaninduced pluripotent stem (hiPS) cell-derived cardiomyocytes and hiPScell-derived NPs (hiPS-NPs)...
August 2018: Tissue & Cell
Nannan Shi, Xiaojing Chen, Rong Liu, Danping Wang, Min Su, Qiang Wang, Aiqin He, Hongmei Gu
Eukaryotic elongation factors 2 (eEF2) plays an essential role in the GTP-dependent translocation of the ribosome along mRNA. Previous studies have shown that eEF2 is overexpressed in various tumors. However, little is known about the role of eEF2 in ovarian cancer. The aim of the present study is to examine the effect of eEF2 on ovarian cancer proliferation. We first measured eEF2 protein expression by western blot using six fresh ovarian cancer tissues from G1 to G3. The results showed that eEF2 expression gradually increased from G1 to G3...
August 2018: Tissue & Cell
Amany Mohamed Shalaby, Noha Gamal Bahey
Nandrolone decanoate is an anabolic-androgenic steroid that is frequently used at a very high dose to improve the physical performance. Recently, this drug has been abused by athletes to augment their muscle mass and improve their physical performance. However, this could have an impact on other body systems with the potential increase in its harmful effect. Therefore, the aim of this study was to evaluate the effect of administering a supraphysiological dose of nandrolone decanoate on the hepatic functions and structure of the adult rat and to test the potential reversibility after nandrolone withdrawal...
August 2018: Tissue & Cell
Min Sun, Xiao-Lin Du, Jun-Qing Liu, Hans-Uwe Dahms, Lan Wang
The morphological and cytological changes of oogenesis and ovarian development were described in the freshwater crab Sinopotamon henanense through macroscopic and microscopic examinations. Serial histological dissections of the ovaries demonstrated that oocyte development was asynchronous. Oogenesis was divided into four distinct stages including six phases: oogonium stage, the first phase (OI) and the second phase (OII) comprising the previtellogenic stage, the third phase (OIII), the fourth phase (OIV) and the fifth phase (OV), comprising the vitellogenic stage and the sixth phase representing the mature stage...
August 2018: Tissue & Cell
Tasuku Kiyuna, Takashi Murakami, Yasunori Tome, Kentaro Igarashi, Kei Kawaguchi, Kentaro Miyake, Masuyo Miyake, Yunfeng Li, Scott D Nelson, Sarah M Dry, Arun S Singh, Tara A Russell, Shree Ram Singh, Fuminori Kanaya, Fritz C Eilber, Robert M Hoffman
Pleomorphic liposarcoma (PLPS) is a heterogeneous resistant group of tumors. Complete surgical resection is the only known way to treat PLPS. PLPS is reristant to both radiation and chemotherapy. Therefore, precise individualized therapy is needed to improve outcome of advanced PLPS patients. In this study, a patient-derived orthotopic xenograft (PDOX) model of a PDGFRA-amplified PLPS was established in the biceps femoris of nude mice by surgical orthotopic implantation (SOI) in order to match the patient. The PLPS PDOX was treated with pazopanib (PAZ) which targets PDGFRA, as well as with temozolomide (TEM) and first-line therapy doxorubicin (DOX)...
August 2018: Tissue & Cell
Xudong Wang, Haizhu Wang, Shutao Zhao, Pengda Sun, Dacheng Wen, Tianzhou Liu, Hao Liu, Zecheng Yang, Zhiming Ma
Eukaryotic translation initiation factor 3 subunit H (EIF3H) is required for the progression of several types of cancer. However, little is known about the function of EIF3H in gastric carcinoma. To address this issue, in the present study, we investigated EIF3H genetic alterations in and expression of EIF3H in gastric cancer tissue samples using cBioPortal and Oncomine databases. Endogenous EIF3H expression was knocked down in MGC80-3 and AGS gastric cancer cell lines by lentivirus-mediated RNA interference...
August 2018: Tissue & Cell
Zhaohui Yu, Hua Xu, Hailin Wang, Youhua Wang
Forkhead box c1 (Foxc1) is a vital member of the Fox family of transcription factors which play important roles in numerous biological processes including metabolism, differentiation, proliferation, apoptosis, migration, invasion and longevity. However, up to date, the role of Foxc1 in the development of Rheumatoid Arthritis (RA) has not been fully elucidated. In the present study, the markedly higher expression of Foxc1 was observed in fibroblast-like synoviocytes (FLSs) of RA compared to control. Besides, we found that Foxc1 had a co-localization with THY1 (a marker for fibroblast-like synoviocytes)...
August 2018: Tissue & Cell
Dev Kumar Das, Surajit Bose, Asok Kumar Maiti, Bhaskar Mitra, Gopeswar Mukherjee, Pranab Kumar Dutta
Identification of various constituent layers such as epithelial, subepithelial, and keratin of oral mucosa and characterization of keratin pearls within keratin region as well, are the important and mandatory tasks for clinicians during the diagnosis of different stages in oral cancer (such as precancerous and cancerous). The architectural variations of epithelial layers and the presence of keratin pearls, which can be observed in microscopic images, are the key visual features in oral cancer diagnosis. The computer aided tool doing the same identification task would certainly provide crucial aid to clinicians for evaluation of histological images during diagnosis...
August 2018: Tissue & Cell
M V T Araujo, M A Spadella, A B Chies, G V Arruda, T de M Santos, M M Cavariani, R F Domeniconi
Head and neck cancers are common in several regions of the world and the treatment usually includes radiotherapy. This treatment can generate adverse effects to the salivary flow, with a relationship between the dose and the damage caused. Salivary gland cells are highly permeable to water and therefore, they express aquaporins (AQPs). This study analyzed changes in the expression and location of these proteins and identified morphological changes induced by low radiation in rat submandibular gland. Female rats were divided into control and irradiated groups...
August 2018: Tissue & Cell
Naoki Hiraishi, Masanori Terai, Mutsunori Fujiwara, Junko Aida, Naotaka Izumiyama-Shimomura, Naoshi Ishikawa, Ken-Ichiro Tomita, Yoko Matsuda, Tomio Arai, Kaiyo Takubo, Toshiyuki Ishiwata
In order to investigate the population dynamics of telomere status, we measured the telomere lengths of glandular cells in the adenohypophysis (AH) and pituicytes, a type of glial cell, in the neurohypophysis (NH) of 128 autopsied humans (65 men, 63 women, 0 and 102 years) using our original quantitative fluorescence in situ hybridization (Q-FISH) method. Telomeres in the AH shortened with aging in both men and women, but those of pituicytes did not. Pituicyte telomeres were significantly longer in women than in men...
August 2018: Tissue & Cell
T Martinovic, D Ciric, I Pantic, K Lalic, I Rasulic, S Despotovic, I Lalic, D Djuricic, V Bumbasirevic, T Kravic-Stevovic
Type 2 diabetes is an endocrine disorder characterized with hyperglycemia, hyperinsulinemia and insulin resistance. Morphological changes in cell nuclei in diabetes were recently detected. The aim of this study was to compare electron microscopic features of lymphocyte nuclei in type 2 diabetes and healthy individuals using conventional computer assisted methods, fractal analysis and gray level co-occurrence matrix (GLCM) analysis of nuclear chromatin. Mononuclear cells taken from the peripheral blood of newly diagnosed type 2 diabetes patients, metformin treated type 2 diabetes patients and healthy individuals were analyzed with transmission electron microscope...
June 2018: Tissue & Cell
Seong Yeong An, Jung Sun Heo
This study examined the effects of low oxygen tension on the osteogenic differentiation of embryonic stem cells (ESCs) in a three-dimensional culture system. The high expression levels of hypoxia-related proteins hypoxia-inducible factor-1α and vascular endothelial growth factor were first validated in ESCs subjected to hypoxic conditions compared with normoxic controls. The osteogenic differentiation of hypoxic ESCs with either osteogenic or osteogenic factor-free media was subsequently evaluated by measuring alkaline phosphatase activity, intracellular calcium levels, matrix mineralization, and the protein levels of osteogenic markers Runt-related transcription factor 2 and osterix...
June 2018: Tissue & Cell
Malgorzata Kotula-Balak, Agnieszka Milon, Piotr Pawlicki, Malgorzata Opydo-Chanek, Anna Pacwa, Klaudia Lesniak, Malgorzata Sekula, Marta Zarzycka, Monika Bubka, Waclaw Tworzydlo, Barbara Bilinska, Anna Hejmej
In this study, we demonstrate, for the first time, estrogen-related receptor (ERR) regulation of the physiological and biochemical status of testicular tumor Leydig cells. In a mouse tumor Leydig cells, ERRs (α, β, and γ) were silenced via siRNA. Cell morphology and cell physiology (proliferation and observation of monolayer formation) were performed by inverted phase-contrast microscope. Leydig cell functional markers (steroid receptors and signaling molecules) were examined by immunofluorescence and Western blotting...
June 2018: Tissue & Cell
Thafar Almela, Sarmad Al-Sahaf, Ian M Brook, Kimia Khoshroo, Morteza Rasoulianboroujeni, Farahnaz Fahimipour, Mohammadreza Tahriri, Erfan Dashtimoghadam, Robert Bolt, Lobat Tayebi, Keyvan Moharamzadeh
Recent advances in three-dimensional printing technology have led to a rapid expansion of its applications in tissue engineering. The present study was designed to develop and characterize an in vitro multi-layered human alveolar bone, based on a 3D printed scaffold, combined with tissue engineered oral mucosal model. The objective was to incorporate oral squamous cell carcinoma (OSCC) cell line spheroids to the 3D model at different anatomical levels to represent different stages of oral cancer. Histological evaluation of the 3D tissue model revealed a tri-layered structure consisting of distinct epithelial, connective tissue, and bone layers; replicating normal oral tissue architecture...
June 2018: Tissue & Cell
Ramesh Periasamy, Daniel V Surbek, Andreina Schoeberlein
The loss of oligodendrocyte progenitor cells (OPC) is a hallmark of perinatal brain injury. Our aim was to develop an in vitro culture condition for human chorion-derived mesenchymal stem cells (MSC) that enhances their stem cell properties and their capability to differentiate towards OPC-like cells. MSC were grown either in serum replacement medium (SRM) or serum-containing medium (SM) and tested for their morphology, proliferation, secretome, migration, protein expression and differentiation into OPC-like cells...
June 2018: Tissue & Cell
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