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Journal of Membrane Biology

Ignat Printsev, Daniel Curiel, Kermit L Carraway
The canonical function of the endoplasmic reticulum-associated degradation (ERAD) system is to enforce quality control among membrane-associated proteins by targeting misfolded secreted, intra-organellar, and intramembrane proteins for degradation. However, increasing evidence suggests that ERAD additionally functions in maintaining appropriate levels of a subset of membrane-associated proteins. In this 'quantity control' capacity, ERAD responds to environmental cues to regulate the proteasomal degradation of specific ERAD substrates according to cellular need...
October 14, 2016: Journal of Membrane Biology
Mario Suwalsky, Pablo Zambrano, María José Gallardo, Fernando Villena, Malgorzata Jemiola-Rzeminska, Kazimierz Strzalka
Thimerosal (THI, ethyl-mercury thiosalicylate) is added to vaccines as a preservative; as a consequence, infants may have been exposed to bolus doses of Hg that collectively added up to nominally 200 µg Hg during the first 6 months of life. While several studies report an association between THI-containing vaccines and neurological disorders, other studies do not support the causal relation between THI and autism. With the purpose to understand the molecular mechanisms of the toxic effect of THI it was assayed on human red cells and in bilayers built-up of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), classes of phospholipids found in the outer and inner monolayers of the human erythrocyte membrane, respectively...
October 13, 2016: Journal of Membrane Biology
Mamatha M Pillai, J Gopinathan, B Indumathi, Y R Manjoosha, K Santosh Sahanand, B K Dinakar Rai, R Selvakumar, Amitava Bhattacharyya
In this study, silk fibroin nanofibrous scaffolds were developed to investigate the attachment and proliferation of primary human meniscal cells. Silk fibroin (SF)-polyvinyl alcohol (PVA) blended electrospun nanofibrous scaffolds with different blend ratios (2:1, 3:1, and 4:1) were prepared. Morphology of the scaffolds was characterized using atomic force microscopy (AFM). The hybrid nanofibrous mats were crosslinked using 25 % (v/v) glutaraldehyde vapor. In degradation study, the crosslinked nanofiber showed slow degradation of 20 % on weight after 35 days of incubation in simulated body fluid (SBF)...
October 11, 2016: Journal of Membrane Biology
Anatoly Kataev, Olga Zherelova, Valery Grishchenko
Interaction of a HAMLET-like La-OA cytotoxic complex (human α-lactalbumin-oleic acid) and its constituents with the excitable plasmalemma of giant Chara corallina cells was investigated. The voltage-clamp technique was used to study Ca(2+) and Cl(-) transient currents in the plasmalemma of intact cells. The action of the complex and OA on the target cell membrane has a dose-dependent character. It was found that the La-OA complex has an inhibiting effect on Ca(2+) current across the plasmalemma, while α-lactalbumin alone does not affect the electrophysiological characteristics of the cellular membrane...
September 16, 2016: Journal of Membrane Biology
Carlos de la Haba, Antoni Morros, Paz Martínez, José R Palacio
Macrophage activation is essential for a correct and efficient response of innate immunity. During oxidative stress membrane receptors and/or membrane lipid dynamics can be altered, leading to dysfunctional cell responses. Our aim is to analyze membrane fluidity modifications and cell function under oxidative stress in LPS-activated macrophages. Membrane fluidity of individual living THP-1 macrophages was evaluated by the technique two-photon microscopy. LPS-activated macrophage function was determined by TNFα secretion...
September 12, 2016: Journal of Membrane Biology
Luz M López-Marín, Blanca E Millán-Chiu, Karen Castaño-González, Carmen Aceves, Francisco Fernández, Alfredo Varela-Echavarría, Achim M Loske
No abstract text is available yet for this article.
September 7, 2016: Journal of Membrane Biology
Svetlana S Efimova, Anastasiia A Zakharova, Ludmila V Schagina, Olga S Ostroumova
The effects of local anesthetics (LAs), including aminoamides and aminoesters, on the characteristics of single gramicidin A (GA) channels in 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) bilayers were studied. Aminoamides, namely lidocaine (LDC), prilocaine (PLC), mepivacaine (MPV), and bupivacaine (BPV), reduced the conductance of GA channels. Aminoesters influenced the current fluctuations induced by GA differently; procaine (PC) did not affect the fluctuations, whereas tetracaine (TTC) distinctly reduced the conductance of single GA channels...
September 3, 2016: Journal of Membrane Biology
Ross G Johnson, Hung C Le, Kristen Evenson, Shelby W Loberg, Tori M Myslajek, Andrea Prabhu, Ann-Marie Manley, Colette O'Shea, Haiying Grunenwald, Madelaine Haddican, Patrick M Fitzgerald, Timothy Robinson, Bruno A Cisterna, Juan C Sáez, Tai-Feng Liu, Dale W Laird, Judson D Sheridan
It is now clear that connexin-based, gap junction "hemichannels" in an undocked state are capable of opening and connecting cytoplasm to the extracellular milieu. Varied studies also suggest that such channel activity plays a vital role in diverse cell processes and abnormal hemichannel activity contributes to pathogenesis. To pursue fundamental questions in this area, investigators require methods for studying hemichannel permeability and dynamics that are quantitative, sensitive, versatile, and available to most cellular and molecular laboratories...
September 1, 2016: Journal of Membrane Biology
Mario Suwalsky, José Colina, María José Gallardo, Malgorzata Jemiola-Rzeminska, Kazimierz Strzalka, Marcela Manrique-Moreno, Benjamín Sepúlveda
Gallic acid (GA) is a polyphenol present in many plants. This study was aimed to investigate the molecular interaction of GA with the human erythrocyte membrane and to determine its antioxidant capacity. The molecular interaction with the membrane of human red cells and the antioxidant property was assayed on both human red cells and molecular models of its membrane. Observations by optical, scanning electron, and defocusing microscopy demonstrated that GA is capable to convert red cells from their normal biconcave shape to crenated echinocytes...
August 27, 2016: Journal of Membrane Biology
Rabea M Wagner, Lara Kricks, Daniel Lopez
Membrane organization is usually associated with the correct function of a number of cellular processes in eukaryotic cells as diverse as signal transduction, protein sorting, membrane trafficking, or pathogen invasion. It has been recently discovered that bacterial membranes are able to compartmentalize their signal transduction pathways in functional membrane microdomains (FMMs). In this review article, we discuss the biological significance of the existence of FMMs in bacteria and comment on possible beneficial roles that FMMs play on the harbored signal transduction cascades...
August 26, 2016: Journal of Membrane Biology
Agnese Denzi, Elena Della Valle, Francesca Apollonio, Marie Breton, Lluis M Mir, Micaela Liberti
Smart drug delivery systems represent an interesting tool to significantly improve the efficiency and the precision in the treatment of a broad category of diseases. In this context, a drug delivery mediated by nanosecond pulsed electric fields seems a promising technique, allowing for a controlled release and uptake of drugs by the synergy between the electropulsation and nanocarriers with encapsulated drugs. The main concern about the use of electroporation for drug delivery applications is the difference in dimension between the liposome (nanometer range) and the cell (micrometer range)...
August 25, 2016: Journal of Membrane Biology
Luz M López-Marín, Blanca E Millán-Chiu, Karen Castaño-González, Carmen Aceves, Francisco Fernández, Alfredo Varela-Echavarría, Achim M Loske
Shock waves are known to permeabilize eukaryotic cell membranes, which may be a powerful tool for a variety of drug delivery applications. However, the mechanisms involved in shock wave-mediated membrane permeabilization are still poorly understood. In this study, the effects on both the permeability and the ultrastructural features of two human cell lineages were investigated after the application of underwater shock waves in vitro. Scanning Electron Microscopy of cells derived from a human embryo kidney (HEK)-293 and Michigan Cancer Foundation (MCF)-7 cells, an immortalized culture derived from human breast adenocarcinoma, showed a small amount of microvilli (as compared to control cells), the presence of hole-like structures, and a decrease in cell size after shock wave exposure...
August 22, 2016: Journal of Membrane Biology
Lu Lai, Ya-Ping Li, Ping Mei, Wu Chen, Feng-Lei Jiang, Yi Liu
The mitochondrial toxicity induced by GSH-CdTe Quantum dots (QDs) of different sizes was investigated. The decreases in absorbance and transmission electron microscopy images show that QDs induce the swelling of mitochondria. Results of flow cytometry indicate that QDs cause a reduction of mitochondrial membrane potential (MMP). A remarkable increase in fluidity of protein regions of mitochondrial membrane is observed, whereas the lipid regions are not obviously affected. Cyclosporin A (CsA) effectively prevents the QD-induced mitochondrial swelling...
August 10, 2016: Journal of Membrane Biology
Qiongxian Yan, Shaoxun Tang, Xuefeng Han, Musibau Adungbe Bamikole, Chuanshe Zhou, Jinhe Kang, Min Wang, Zhiliang Tan
Free fatty acids (FFAs) in plasma are essential substrates for de novo synthesis of milk fat, or directly import into mammary cells. The physico-chemical properties of mammary cells membrane composition affected by FFAs with different chain lengths and saturability are unclear yet. Employing GC, FTIR and fluorescence spectroscopy, the adsorption capacity, phospholipids content, membrane proteins conformation, lipid peroxidation product, and free sulfhydryl of plasma membranes (PMs) interacted with different FFAs were determined...
July 28, 2016: Journal of Membrane Biology
P Thomas Vernier, Lluis M Mir, Damijan Miklavčič
No abstract text is available yet for this article.
October 2016: Journal of Membrane Biology
Lukas J Motloch, Tina Gebing, Sara Reda, Astrid Schwaiger, Martin Wolny, Uta C Hoppe
Mitochondrial Ca(2+) uptake (mCa(2+) uptake) is thought to be mediated by the mitochondrial Ca(2+) uniporter (MCU). UCP2 and UCP3 belong to a superfamily of mitochondrial ion transporters. Both proteins are expressed in the inner mitochondrial membrane of the heart. Recently, UCP2 was reported to modulate the function of the cardiac MCU related channel mCa1. However, the possible role of UCP3 in modulating cardiac mCa(2+) uptake via the MCU remains inconclusive. To understand the role of UCP3, we analyzed cardiac mCa1 single-channel activity in mitoplast-attached single-channel recordings from isolated murine cardiac mitoplasts, from adult wild-type controls (WT), and from UCP3 knockout mice (UCP3(-/-))...
August 2016: Journal of Membrane Biology
Katarzyna Janicka, Izabella Jastrzebska, Aneta Dorota Petelska
Diosgenin (Dio) has shown many treatment properties, but the most important property is cytotoxic activity in cancer cells. In this study, we investigated monolayers of Dio, cholesterol (Ch), and phosphatidylcholine (PC) at the air/water interface. The measurements were carried with a Langmuir Teflon trough and a Nima 9000 tensiometer program. The surface tension values of pure and mixed monolayers were used to calculate π-A isotherms and determine molecular surface areas. We were able to demonstrate the formation of complexes between Dio and PC and Dio and Ch molecules also...
August 2016: Journal of Membrane Biology
Stine Krog Frandsen, Anna K McNeil, Ivana Novak, Paul L McNeil, Julie Gehl
Electroporation-based treatments and other therapies that permeabilize the plasma membrane have been shown to be more devastating to malignant cells than to normal cells. In this study, we asked if a difference in repair capacity could explain this observed difference in sensitivity. Membrane repair was investigated by disrupting the plasma membrane using laser followed by monitoring fluorescent dye entry over time in seven cancer cell lines, an immortalized cell line, and a normal primary cell line. The kinetics of repair in living cells can be directly recorded using this technique, providing a sensitive index of repair capacity...
August 2016: Journal of Membrane Biology
M Salai Mathi Selvi, G Hariharan
Wavelet method is a recently developed tool in applied mathematics. The mathematical model of the steady-state immobilized enzyme electrodes is discussed. This theoretical model is based on one-dimensional heat conduction equations containing a non-linear term related to Michaelis-Menten kinetics. An efficient Chebyshev wavelet-based technique is applied to solve the non-linear diffusion equation for the steady-state condition. A simple expression of the substrate concentration is obtained as a function of the Thiele modulus [Formula: see text] and [Formula: see text](kinetic parameter)...
August 2016: Journal of Membrane Biology
Carlos A Báez-Pagán, Natalie Del Hoyo-Rivera, Orestes Quesada, José David Otero-Cruz, José A Lasalde-Dominicci
The nicotinic acetylcholine receptor (nAChR), located in the cell membranes of neurons and muscle cells, mediates the transmission of nerve impulses across cholinergic synapses. In addition, the nAChR is also found in the electric organs of electric rays (e.g., the genus Torpedo). Cholesterol, which is a key lipid for maintaining the correct functionality of membrane proteins, has been found to alter the nAChR function. We were thus interested to probe the changes in the functionality of different nAChRs expressed in a model membrane with modified cholesterol to phospholipid ratios (C/P)...
August 2016: Journal of Membrane Biology
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