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Journal of Membrane Biology

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https://www.readbyqxmd.com/read/28647752/possible-effects-of-electric-fields-on-a-pair-of-spherical-cells
#1
Yu Zheng, Jing Xue, Yang Gao, Lei Dong, Jun-Rong Dou, Wei Ma
Electric fields (EF) can induce some physiological or biological effects in neural tissues, which have been explored in many applications such as electroporation. The key to understand the possible underlying mechanisms of such effects tend to be the induced transmembrane potential. Although transmembrane potentials have already been the subject of many theoretical studies, most previous works concerning this topic are mainly focused on the situations of isolated cells. In previous studies, cells are often considered to be three-compartment models with different electroconductivities in different regions (the three compartments are often intracellular regions, membrane, and extracellular regions)...
June 24, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28623474/epithelial-fluid-transport-is-due-to-electro-osmosis-80-plus-osmosis-20
#2
Jorge Fischbarg, Julio A Hernandez, Andrey A Rubashkin, Pavel Iserovich, Veronica I Cacace, Carlos F Kusnier
Epithelial fluid transport, an important physiological process shrouded in a long-standing enigma, may finally be moving closer to a solution. We propose that, for the corneal endothelium, relative proportions for the driving forces for fluid transport are 80% of paracellular electro-osmosis, and 20% classical transcellular osmosis. These operate in a cyclical process with a period of 9.2 s, which is dictated by the decrease and exhaustion of cellular Na(+). Paracellular electro-osmosis is sketched here, and partially discussed as much as the subject still allows; transcellular osmosis is presented at length...
June 16, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28597209/molecular-dynamics-simulation-reveals-unique-interplays-between-a-tarantula-toxin-and-lipid-membranes
#3
Lei Wu, Si-Si Xie, Er Meng, Wen-Ying Li, Long Liu, Dong-Yi Zhang
Tarantula toxins compose an important class of spider toxins that target ion channels, and some are known to interact with lipid membranes. In this study, we focus on a tarantula toxin, Jingzhaotoxin-III (JZTx-III) that specifically targets the cardiac voltage-gated sodium channel Na[Formula: see text]1.5 and is suspected to be able to interact with lipid membranes. Here, we use an all-atom model and long-term molecular dynamics simulations to investigate the interactions between JZTx-III and lipid membranes of different compositions...
June 8, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28488084/loop-diuretics-diminish-hemolysis-induced-by-%C3%AE-hemolysin-from-escherichia-coli
#4
Carl Martin Söderström, Steen K Fagerberg, Mette B Brogaard, Jens Leipziger, Marianne Skals, Helle A Praetorius
Uropathogenic Escherichia coli often produce the virulence factor α-hemolysin (HlyA), and the more severe the infection, the likelier it is to isolate HlyA-producing E. coli from patients. HlyA forms pores upon receptor-independent insertion of the toxin into biological membranes and it has been substantiated that HlyA-induced hemolysis is amplified by toxin-induced ATP release and activation of P2X receptors. Thus, hemolysis inflicted by HlyA is a protracted process involving signal transduction. It consists of early, marked cell shrinkage followed by swelling and eventually lysis...
May 9, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28484798/asymmetric-patterns-of-small-molecule-transport-after-nanosecond-and-microsecond-electropermeabilization
#5
Esin B Sözer, C Florencia Pocetti, P Thomas Vernier
Imaging of fluorescent small molecule transport into electropermeabilized cells reveals polarized patterns of entry, which must reflect in some way the mechanisms of the migration of these molecules across the compromised membrane barrier. In some reports, transport occurs primarily across the areas of the membrane nearest the positive electrode (anode), but in others cathode-facing entry dominates. Here we compare YO-PRO-1, propidium, and calcein uptake into U-937 cells after nanosecond (6 ns) and microsecond (220 µs) electric pulse exposures...
May 8, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28470363/erratum-to-inhibition-of-the-innate-immune-receptors-for-foreign-dna-sensing-improves-transfection-efficiency-of-gene-electrotransfer-in-melanoma-b16f10-cells
#6
Masa Bosnjak, Urska Kamensek, Gregor Sersa, Danijela Stolfa, Jaka Lavrencak, Maja Cemazar
No abstract text is available yet for this article.
May 3, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28456838/theoretical-study-of-molecular-transport-through-a-permeabilized-cell-membrane-in-a-microchannel
#7
Masoumeh Mahboubi, Saeid Movahed, Reza Hosseini Abardeh, Vahid Hoshyargar
A two-dimensional model is developed to study the molecular transport into an immersed cell in a microchannel and to investigate the effects of finite boundary (a cell is suspended in a microchannel), amplitude of electric pulse, and geometrical parameter (microchannel height and size of electrodes) on cell uptake. Embedded electrodes on the walls of the microchannel generate the required electric pulse to permeabilize the cell membrane, pass the ions through the membrane, and transport them into the cell. The shape of electric pulses is square with the time span of 6 ms; their intensities are in the range of 2...
April 29, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28451712/studies-of-lipid-monolayers-prepared-from-native-and-model-plant-membranes-in-their-interaction-with-zearalenone-and-its-mixture-with-selenium-ions
#8
Barbara Gzyl-Malcher, Maria Filek, Elżbieta Rudolphi-Skórska, Apolonia Sieprawska
The impact of zearalenone and selenate ions on the monolayers of 1,2-dipalmitoyl-phosphatidylcholine (DPPC), 1,2-dipalmitoyl-3-trimethylammonium-propane (DPTAP), and the lipid mixtures (phospholipids and galactolipids) extracted from wheat plasmalemma has been studied using Langmuir trough technique and Brewster angle microscopy (BAM). The zearalenone is a mycotoxin that exerts toxic effects on the cells of plants and animals. Monolayers' properties were characterized by surface pressure (π)-molecular area (A) isotherms...
April 27, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28434115/interaction-of-selected-phenylpropenes-with-dipalmitoylphosphatidylcholine-membrane-and-their-relevance-to-antibacterial-activity
#9
Riham Gharib, Amal Najjar, Lizette Auezova, Catherine Charcosset, Hélène Greige-Gerges
The effect of structurally closely related phenylpropenes (PPs), estragole, anethole, eugenol, and isoeugenol, on the fluidity of dipalmitoyl phosphatidyl choline (DPPC) liposome membrane was investigated by DSC, Raman, and fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH). Liposomes were prepared by thin-film hydration method at various DPPC:PP molar ratios. The DPH anisotropy measurements of blank and PP-loaded liposomes were performed at 28, 41, and 50 °C, which correspond, respectively, to gel phase, main transition temperature of DPPC, and liquid phase...
April 22, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28417145/relationship-in-pacemaker-neurons-between-the-long-term-correlations-of-membrane-voltage-fluctuations-and-the-corresponding-duration-of-the-inter-spike-interval
#10
Alberto Seseña Rubfiaro, José Rafael Godínez, Juan Carlos Echeverría
Several studies of the behavior in the voltage and frequency fluctuations of the neural electrical activity have been performed. Here, we explored the particular association between behavior of the voltage fluctuations in the inter-spike segment (VFIS) and the inter-spike intervals (ISI) of F1 pacemaker neurons from H. aspersa, by disturbing the intracellular calcium handling with cadmium and caffeine. The scaling exponent α of the VFIS, as provided by detrended fluctuations analysis, in conjunction with the corresponding duration of ISI to estimate the determination coefficient R (2) (48-50 intervals per neuron, N = 5) were all evaluated...
April 17, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28386629/an-in-vitro-study-of-the-antioxidant-and-antihemolytic-properties-of-buddleja-globosa-matico
#11
Mario Suwalsky, José Duguet, Hernán Speisky
The antioxidant and antihemolytic properties contained in the leaves of Buddleja globosa (B. globosa), also known as "Matico," were determined. Aqueous extracts of leaves were assayed in human erythrocytes and molecular models of its membrane. The latter were bilayers built-up of lipids located in the outer and inner leaflets of the erythrocyte membrane. Observations by scanning electron microscopy showed that the extract altered the morphology of erythrocytes inducing the formation of crenated echinocytes...
April 6, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28243693/effect-of-cooling-on-cell-volume-and-viability-after-nanoelectroporation
#12
Claudia Muratori, Andrei G Pakhomov, Olga N Pakhomova
Electric pulses of nanosecond duration (nsEP) are emerging as a new modality for tissue ablation. Plasma membrane permeabilization by nsEP may cause osmotic imbalance, water uptake, cell swelling, and eventual membrane rupture. The present study was aimed to increase the cytotoxicity of nsEP by fostering water uptake and cell swelling. This aim was accomplished by lowering temperature after nsEP application, which delayed the membrane resealing and/or suppressed the cell volume mechanisms. The cell diameter in U-937 monocytes exposed to a train of 50, 300-ns pulses (100 Hz, 7 kV/cm) at room temperature and then incubated on ice for 30 min increased by 5...
April 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28239748/%C3%AE-synuclein-s-uniquely-long-amphipathic-helix-enhances-its-membrane-binding-and-remodeling-capacity
#13
Anthony R Braun, Michael M Lacy, Vanessa C Ducas, Elizabeth Rhoades, Jonathan N Sachs
α-Synuclein is the primary protein found in Lewy bodies, the protein and lipid aggregates associated with Parkinson's disease and Lewy body dementia. The protein folds into a uniquely long amphipathic α-helix (AH) when bound to a membrane, and at high enough concentrations, it induces large-scale remodeling of membranes (tubulation and vesiculation). By engineering a less hydrophobic variant of α-Synuclein, we previously showed that the energy associated with binding of α-Synuclein's AH correlates with the extent of membrane remodeling (Braun et al...
April 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28280854/deficiency-in-sperm-egg-protein-interaction-as-a-major-cause-of-fertilization-failure
#14
REVIEW
Soudabeh Sabetian, Mohd Shahir Shamsir
Complete elucidation of fertilization process at molecular level is one of the unresolved challenges in sexual reproduction studies, and understanding the molecular mechanism is crucial in overcoming difficulties in infertility and unsuccessful in vitro fertilization. Sperm-oocyte interaction is one of the most remarkable events in fertilization process, and deficiency in protein-protein interactions which mediate this interaction is a major cause of unexplained infertility. Due to detection of how the various defects of sperm-oocyte interaction can affect fertilization failure, different experimental methods have been applied...
March 9, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28251264/calcium-induced-mitochondrial-permeability-transitions-parameters-of-ca-2-ion-interactions-with-mitochondria-and-effects-of-oxidative-agents
#15
Nina G Golovach, Vitali T Cheshchevik, Elena A Lapshina, Tatsiana V Ilyich, Ilya B Zavodnik
We evaluated the parameters of Ca(2+)-induced mitochondrial permeability transition (MPT) pore formations, Ca(2+) binding constants, stoichiometry, energy of activation, and the effect of oxidative agents, tert-butyl hydroperoxide (tBHP), and hypochlorous acid (HOCl), on Ca(2+) -mediated process in rat liver mitochondria. From the Hill plot of the dependence of MPT rate on Ca(2+) concentration, we determined the order of interaction of Ca(2+) ions with the mitochondrial sites, n = 3, and the apparent Kd = 60 ± 12 µM...
March 1, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28238117/membrane-permeabilization-of-pathogenic-yeast-in-alternating-sub-microsecond-electromagnetic-fields-in-combination-with-conventional-electroporation
#16
Vitalij Novickij, Eglė Lastauskienė, Jurgita Švedienė, Audrius Grainys, Gediminas Staigvila, Algimantas Paškevičius, Irutė Girkontaitė, Auksė Zinkevičienė, Svetlana Markovskaja, Jurij Novickij
Recently, a novel contactless treatment method based on high-power pulsed electromagnetic fields (PEMF) was proposed, which results in cell membrane permeabilization effects similar to electroporation. In this work, a new PEMF generator based on multi-stage Marx circuit topology, which is capable of delivering 3.3 T, 0.19 kV/cm sub-microsecond pulses was used to permeabilize pathogenic yeast Candida albicans separately and in combination with conventional square wave electroporation (8-17 kV/cm, 100 μs)...
February 25, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28229180/erratum-to-high-resolution-structure-of-a-membrane-protein-transferred-from-amphipol-to-a-lipidic-mesophase
#17
V Polovinkin, I Gushchin, M Sintsov, E Round, T Balandin, P Chervakov, V Shevchenko, P Utrobin, A Popov, V Borshchevskiy, A Mishin, A Kuklin, D Willbold, V Chupin, J-L Popot, V Gordeliy
No abstract text is available yet for this article.
February 22, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28224174/spectroscopic-polarographic-and-microcalorimetric-studies-on-mitochondrial-dysfunction-induced-by-ethanol
#18
Long Ma, Jia-Xin Dong, Can Wu, Xue-Yi Li, Jing Chen, Hong Zhang, Yi Liu
Liver mitochondria are involved in several important life processes; mitochondrial dysfunction and disorders are implicated in several human diseases. Alcohol permeates all tissues of the body and exerts some intrinsic hepatotoxicity. In this work, our results demonstrated that ethanol caused a series of mitochondria permeability transition pore (MPTP) opening factors such as mitochondrial swelling, increased permeability of H(+) and K(+), collapsed membrane potential, and increased membrane fluidity. Furthermore, mitochondrial ultrastructure alternation observed clearly by transmission electron microscopy and the release of Cytochrome c could explain the MPTP opening from another aspect...
February 21, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28214974/interaction-of-scots-pine-defensin-with-model-membrane-by-coarse-grained-molecular-dynamics
#19
Elena Ermakova, Yuriy Zuev
Plant defensins are a part of the innate immune system of plants that acts against a broad range of pathogens. Many plant defensins, including pine defensins, show strong antifungal activity that is associated with their ability to penetrate into the fungal cell membrane. However, the exact molecular mechanism of their action remains poorly defined. To obtain insight into the mechanism of protein-membrane interaction, we applied a coarse-grained molecular dynamics simulation to study the interaction of pine defensin with two model membranes: the first consisted of zwitterion-neutral POPC molecules and the second was composed of combined anionic POPG and POPC...
February 18, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28204840/inhibition-of-the-innate-immune-receptors-for-foreign-dna-sensing-improves-transfection-efficiency-of-gene-electrotransfer-in-melanoma-b16f10-cells
#20
Masa Bosnjak, Urska Kamensek, Gregor Sersa, Danijela Stolfa, Jaka Lavrencak, Maja Cemazar
Gene electrotransfer upregulate DNA pattern recognition receptors or DNA sensors, which are part of the innate immune system. In this study, we tested if addition of the cocktail of innate immune system inhibitors to the cells during gene electrotransfer (GET) can increase transfection efficiency and cell survival. The results indicate that this cocktail can decrease cytosolic DNA sensors expression after GET, and consequently increase cell survival and transfection efficiency in B16 cells, but only in highly metastatic B16F10 subtype...
February 15, 2017: Journal of Membrane Biology
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