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Journal of Membrane Biology

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https://www.readbyqxmd.com/read/28914342/quantification-of-the-intracellular-life-time-of-water-molecules-to-measure-transport-rates-of-human-aquaglyceroporins
#1
Madelene Palmgren, Malin Hernebring, Stefanie Eriksson, Karin Elbing, Cecilia Geijer, Samo Lasič, Peter Dahl, Jesper S Hansen, Daniel Topgaard, Karin Lindkvist-Petersson
Orthodox aquaporins are transmembrane channel proteins that facilitate rapid diffusion of water, while aquaglyceroporins facilitate the diffusion of small uncharged molecules such as glycerol and arsenic trioxide. Aquaglyceroporins play important roles in human physiology, in particular for glycerol metabolism and arsenic detoxification. We have developed a unique system applying the strain of the yeast Pichia pastoris, where the endogenous aquaporins/aquaglyceroporins have been removed and human aquaglyceroporins AQP3, AQP7, and AQP9 are recombinantly expressed enabling comparative permeability measurements between the expressed proteins...
September 15, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28894900/interaction-between-luteinizing-hormone-releasing-hormone-and-gm1-doped-cholesterol-sphingomyelin-vesicles-a-spectroscopic-study
#2
Zarrin Shahzadi, Chaitali Mukhopadhyay
Understanding the role of neural membrane in translocation and action of neurohormone is of great importance. Luteinizing hormone-releasing hormone (LHRH) is a neuropeptide hormone and it acts as a final signaling molecule by stimulating the synthesis of LH and FSH to maintain reproduction in all vertebrates. The receptors of LHRH are found in breast tumors and pituitary gland in the brain. Moreover, neural plasma membrane is also found to contain specific binding site for LHRH. The mechanism by which LHRH binds to membrane before it binds to the receptors is a very critical step and can have a profound impact upon the translation of peptide across the membrane...
September 11, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28887642/evaluation-of-the-functional-capacity-of-nk-cells-of-melanoma-patients-in-an-in-vitro-model-of-nk-cell-contact-with-k562-and-femx-tumor-cell-lines
#3
Gordana Konjevic, Ana Vuletic, Katarina Mirjacic Martinovic, Ana Krivokuca, Radmila Jankovic, Nada Babovic
NK cells of metastatic melanoma (MM) patients display impaired function, making them incapable to mount an effective antitumor response. In this study, we evaluated immunophenotypic characteristics and functional capacity of CD3(-)CD16(+) NK cells of MM patients in an in vitro model based on NK cell contact with an NK sensitive, K562, and a tumor-specific, melanoma FemX tumor cell line. Although our results indicate similar NK cell antitumor cytotoxic potential of MM patients in contact with both cell lines based on the expression of CD107a degranulation marker, there is a discrepancy in NK cell IFNγ production, as it is not significantly induced by FemX tumor cells, found to be, contrary to K562, HLA class I positive...
September 8, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28871398/effect-of-pulsed-electric-fields-pef-on-accumulation-of-magnesium-in-lactobacillus-rhamnosus-b-442-cells
#4
Małgorzata Góral, Urszula Pankiewicz
The aim of this study was to determine the effect of pulsed electric fields (PEF) on accumulation of magnesium ions in Lactobacillus rhamnosus B 442 cells. Under optimized conditions, this is, on 15 min exposure of the 20 h grown culture to PEF of the 2.0 kV/cm and 20 µs pulse width at concentration 400 μg Mg(2+)/mL medium, accumulation of magnesium in the biomass reached maximum 4.28 mg/g d.m. Optimization of PEF parameters caused an increase of magnesium concentration in the cells by 220% in comparison to the control not treated with PEF...
September 4, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28866739/effect-of-sodium-chloride-on-surface-associated-motility-of-acinetobacter-baumannii-and-the-role-of-aders-two-component-system
#5
P Malaka De Silva, Ayush Kumar
Acinetobacter baumannii is an important bacterial pathogen whose resistance to antibiotics is a growing concern worldwide. Among a wide array of resistance mechanisms displayed by A. baumannii, energy-dependent efflux of antibiotics by proteins belonging the resistance-nodulation-division family serves as an important one. AdeABC pump has been shown to be active in various clinical isolates. Regulation of this pump is controlled by the AdeRS two-component system. In this study, we show that the AdeRS system, in addition to modulating A...
September 2, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28861595/oxysterols-versus-cholesterol-in-model-neuronal-membrane-i-the-case-of-7-ketocholesterol-the-langmuir-monolayer-study
#6
Anita Wnętrzak, Katarzyna Makyła-Juzak, Anna Filiczkowska, Waldemar Kulig, Patrycja Dynarowicz-Łątka
Oxysterols are products of cholesterol oxidation. They can be formed endogenously (in both enzymatic and non-enzymatic reactions) as well as exogenously (delivered with food). Recent studies clearly demonstrate cytotoxic properties of these compounds, being mainly due to their incorporation into natural lipid bilayers. This process can influence mechanical and physicochemical properties of biomembrane-mainly by modifying the interactions between its components, which may result in the disruption of proper functioning of cell membrane and could lead to its degradation...
August 31, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28852815/mycobacterium-tuberculosis-major-facilitator-superfamily-transporters
#7
REVIEW
Ping Li, Yinzhong Gu, Jiang Li, Longxiang Xie, Xue Li, Jianping Xie
The cell membrane or biofilm serve as permeable barrier for xenobiotics to maintain the homeostasis of cells or bacterial community. Transport systems are essential for the uptake of nutrients and substances necessary for biofilm formation, efflux of deleterious compounds, as well as communication between cells and environment. Major facilitator superfamily (MFS) represents the largest secondary transporter family and is responsible for the transport of a broad spectrum of substrates with diverse physiochemical properties by utilizing the energy stored in electrochemical gradient across the membrane...
August 29, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28852814/identification-of-resveratrol-an-herbal-compound-as-an-activator-of-the-calcium-activated-chloride-channel-tmem16a
#8
Ran Chai, Yafei Chen, Hongbo Yuan, Xuzhao Wang, Shuai Guo, Jinlong Qi, Hailin Zhang, Yong Zhan, Hailong An
Calcium-activated chloride channels (CaCCs) play vital roles in a variety of physiological processes. Dysfunction of the CaCCs is implicated in many diseases. Drug discovery targeting at CaCCs has recently become possible with the determination that TMEM16A is the molecular identity of CaCCs. In this study, we demonstrated that resveratrol (RES), a Chinese traditional medicine compound, is a novel activator of TMEM16A. The yellow fluorescence protein quenching assay and measurement of intracellular calcium fluorescence intensity show that RES activates TMEM16A channels in an intracellular Ca(2+)-independent way...
August 29, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28840286/adrenal-chromaffin-cells-exposed-to-5-ns-pulses-require-higher-electric-fields-to-porate-intracellular-membranes-than-the-plasma-membrane-an-experimental-and-modeling-study
#9
Josette Zaklit, Gale L Craviso, Normand Leblanc, Lisha Yang, P Thomas Vernier, Indira Chatterjee
Nanosecond-duration electric pulses (NEPs) can permeabilize the endoplasmic reticulum (ER), causing release of Ca(2+) into the cytoplasm. This study used experimentation coupled with numerical modeling to understand the lack of Ca(2+) mobilization from Ca(2+)-storing organelles in catecholamine-secreting adrenal chromaffin cells exposed to 5-ns pulses. Fluorescence imaging determined a threshold electric (E) field of 8 MV/m for mobilizing intracellular Ca(2+) whereas whole-cell recordings of membrane conductance determined a threshold E-field of 3 MV/m for causing plasma membrane permeabilization...
August 24, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28840273/a-kinetic-characterization-of-the-gill-na-k-atpase-from-the-semi-terrestrial-mangrove-crab-cardisoma-guanhumi-latreille-1825-decapoda-brachyura
#10
Daniel L Farias, Malson N Lucena, Daniela P Garçon, Fernando L Mantelatto, John C McNamara, Francisco A Leone
We provide a kinetic characterization of (Na(+), K(+))-ATPase activity in a posterior gill microsomal fraction from the semi-terrestrial mangrove crab Cardisoma guanhumi. Sucrose density gradient centrifugation reveals two distinct membrane fractions showing considerable (Na(+), K(+))-ATPase activity, but also containing other microsomal ATPases. The (Na(+), K(+))-ATPase, notably immuno-localized to the apical region of the epithelial pillar cells, and throughout the pillar cell bodies, has an M r of around 110 kDa and hydrolyzes ATP with V M = 146...
August 24, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28823021/control-by-low-levels-of-calcium-of-mammalian-cell-membrane-electropermeabilization
#11
Ciobanu Florin, Golzio Muriel, Kovacs Eugenia, Teissié Justin
Electric pulses, when applied to a cell suspension, induce a reversible permeabilization of the plasma membrane. This permeabilized state is a long-lived process (minutes). The biophysical molecular mechanisms supporting the membrane reorganization associated to its permeabilization remain poorly understood. Modeling the transmembrane structures as toroidal lipidic pores cannot explain why they are long-lived and why their resealing is under the control of the ATP level. Our results describe the effect of the level of free Calcium ions...
August 20, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28821926/insights-into-signaling-and-the-functional-complexity-of-biological-membranes
#12
EDITORIAL
Leonor Saiz
No abstract text is available yet for this article.
August 18, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28815271/the-prolidase-activity-oxidative-stress-and-nitric-oxide-levels-of-bladder-tissues-with-or-without-tumor-in-patients-with-bladder-cancer
#13
İlhan Gecit, Recep Eryılmaz, Servet Kavak, İsmail Meral, Halit Demir, Necip Pirinççi, Mustafa Güneş, Kerem Taken
This study was designed to evaluate the malondialdehyde (MDA), glutathione (GSH) and nitric oxide (NO) levels, and also prolidase, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) enzyme activities in malignant and benign cancers of bladder tissue. A total of 59 patients admitted to our clinic due to microscopic or macroscopic haematuria, were prospectively included in the study. Because of some reasons (no request to participate in the study, the inability to reach, other malignancies, alcohol consumption, metabolic disease), eight patients were excluded from study...
August 16, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28799139/the-effects-of-the-structure-and-composition-of-the-hydrophobic-parts-of-phosphatidylcholine-containing-systems-on-phosphatidylcholine-oxidation-by-ozone
#14
Elżbieta Rudolphi-Skórska, Maria Filek, Maria Zembala
The degree of lipid unsaturation is a parameter used to describe membrane susceptibility to oxidation. This paper highlights the importance of double bond distribution in the hydrophobic parts of lipid layers. The problem was studied by determining the effects induced by ozone dissolved in an aqueous phase acting on layers of unsaturated cholines of various molecular structures, including bi-unsaturated (DOPC), mono-unsaturated (POPC) and natural origin (soy PC). The destructive effects of ozone were quantified as the ratio of areas per molecule, which corresponded to a 1 mN/m rise in the layer surface pressure for oxidized to non-oxidized lipids (A lift/A lift(0) )...
August 10, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28776087/efficacy-of-in-vivo-electroporation-mediated-il-10-gene-delivery-on-survival-of-skin-flaps
#15
S Morteza Seyed Jafari, Maziar Shafighi, Helmut Beltraminelli, Benedikt Weber, Ralph A Schmid, Thomas Geiser, Amiq Gazdhar, Robert E Hunger
Despite advances in understanding the underlying mechanisms of flap necrosis and improvement in surgical techniques, skin flap necrosis after reconstructive surgery remains a crucial issue. We investigated the efficacy of electroporation-mediated IL-10 gene transfer to random skin flap with an aim to accelerate wound healing and improve skin flap survival. Nine male Wistar rats (300-330 g) were divided in two groups (a) control group (n = 5), only surgery no gene transfer, and (b) experimental group, received electroporation-mediated IL-10 gene transfer 24 h before the surgery as prophylaxis (n = 4)...
August 3, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28766006/tissue-level-cardiac-electrophysiology-studied-in-murine-myocardium-using-a-microelectrode-array-autonomic-and-thermal-modulation
#16
Jem D Lane, David Montaigne, Andrew Tinker
Cardiac electrophysiology is regulated by the autonomic nervous system, and this has both pathophysiological, and possibly therapeutic importance. Furthermore, chamber differences in electrophysiology exist between atria and ventricles, yet there have been few direct comparisons. There is substantial literature on ion channel modulation at the single-cell level but less work on how this affects tissue-level parameters. We used a microelectrode array system to explore these issues using murine atrial and ventricular tissue slices...
August 1, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28741121/interaction-of-newly-platinum-ii-with-tris-2-carboxyethyl-phosphine-complex-with-dna-and-model-lipid-membrane
#17
Hanna Pruchnik, Teresa Kral, Martin Hof
Structural properties of plasmid DNA and model lipid membrane treated with newly synthesized platinum(II) complex cis-[PtCl2{P(CH2CH2COOH)3}2] (cis-DTCEP for short) were studied and compared with effects of anticancer drug cisplatin, cis-[Pt(NH3)2Cl2] (cis-DDP for short). Time Correlated Single Photon Counting Fluorescence Correlation Spectroscopy (TCSPC-FCS) was employed to study interactions between those platinum complexes and DNA. The TCSPC-FCS results suggest that bonding of cis-DTCEP derivative to DNA leads to plasmid strain realignment towards much more compact structure than in the case of cis-DDP...
July 25, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28735341/the-effect-of-nanosecond-high-voltage-electric-pulses-on-the-shape-and-permeability-of-polymersome-guvs
#18
Tina Batista Napotnik, Gianluca Bello, Eva-Kathrin Sinner, Damijan Miklavčič
Polymersomes, vesicles composed of block copolymers, are promising candidates as membrane alternatives and functional containers, e.g., as potential carriers for functional molecules because of their stability and tunable membrane properties. In the scope of possible use for membrane protein delivery to cells by electrofusion, we investigated the cytotoxicity of such polymersomes as well as the effects of nanosecond electric pulses with variable repetition rate on the shape and permeability of polymersomes in buffers with different conductivities...
July 22, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28711950/mathematical-models-of-electrical-activity-in-plants
#19
REVIEW
Ekaterina Sukhova, Elena Akinchits, Vladimir Sukhov
Electrical activity plays an important role in plant life; in particular, electrical responses can participate in the reception of the action of stressors (local electrical responses and oscillations) and signal transduction into unstimulated parts of the plant (action potential, variation potential and system potential). Understanding the mechanisms of electrical responses and subsequent changes in physiological processes and the prediction of plant responses to stressors requires the elaboration of mathematical models of electrical activity in plant organisms...
July 15, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28660286/hydrocinnamic-acid-inhibits-the-currents-of-wt-and-sqt3-syndrome-related-mutants-of-kir2-1-channel
#20
Shuxi Ren, Chunli Pang, Yayue Huang, Chengfen Xing, Yong Zhan, Hailong An
Gain of function in mutations, D172N and E299V, of Kir2.1 will induce type III short QT syndrome. In our previous work, we had identified that a mixture of traditional Chinese medicine, styrax, is a blocker of Kir2.1. Here, we determined a monomer, hydrocinnamic acid (HA), as the effective component from 18 compounds of styrax. Our data show that HA can inhibit the currents of Kir2.1 channel in both excised inside-out and whole-cell patch with the IC50 of 5.21 ± 1.02 and 10.08 ± 0.46 mM, respectively...
June 28, 2017: Journal of Membrane Biology
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