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Journal of Microscopy

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https://www.readbyqxmd.com/read/29786842/correlated-time-variation-of-bulk-microstructure-and-rheology-in-asphalt-binders
#1
A Ramm, N Sakib, A Bhasin, M C Downer
We use near-infrared dark-field optical microscopy to probe isothermal time variation of the volume fraction of naturally-occurring, subsurface microstructures in PG 64-22 asphalt binders at temperature T=30∘C, following a rapid heating (cooling) increment |ΔT|=20∘C from initial temperature T0=10∘C(50∘C). We compare these microstructure variations with isothermal time variations of the magnitude |G30∗(t)| of the bulk complex shear modulus measured for identical sample conditions with a Dynamic Shear Rheometer...
May 22, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29782656/expansion-microscopy
#2
REVIEW
I Cho, J Y Seo, J Chang
Super-resolution optical microscopy techniques have revolutionized how we see and understand biology. In recent past, a new super-resolution optical microscopy technique called expansion microscopy (ExM) was developed. Unlike other pre-existing super-resolution imaging techniques, this technique achieves super-resolution by physically expanding biological specimens via a swellable hydrogel. After the development of ExM, various techniques based on ExM but with improved performance in various aspects, have been developed...
May 21, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29762877/visualisation-of-insect-tracheal-systems-by-lactic-acid-immersion
#3
Y Ruan, Y Li, M Zhang, X Chen, Z Liu, S Wang, S Jiang
The endeavours to reveal the tracheal system of insects and some arachnids has a long history. The traditional way to observe a tracheal system in an insect body is by utilising the glycerin immersion method. In this study, we developed the lactic acid immersion method, which reveals a more complete tracheal system. By mounting various types of live specimens or body parts directly into lactic acid, multiple intact and complex tracheal systems were clearly visualised. The lactic acid immersion contributed to revealing tracheal systems by penetrating body tissue while reserving enough time for observation before the penetration of the tracheae...
May 15, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29762874/direct-manipulation-of-metallic-nanosheets-by-shear-force-microscopy
#4
Z Bi, W Cai, Y Wang, G Shang
Micro/nanomanipulation is a rapidly growing technology and holds promising applications in various fields, including photonic/electronic devices, chemical/biosensors etc. In this work, we present that shear force microscopy (ShFM) can be exploited to manipulate metallic nanosheets besides imaging. The manipulation is realized via controlling the shear force sensor probe position and shear force magnitude based on our homemade ShFM system under an optical microscopy for in situ observation. The main feature of the ShFM system is usage of a piezoelectric bimorph sensor, which has the ability of self-excitation and detection...
May 15, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29727472/local-rotations-due-to-mixed-type-misfit-dislocation-at-%C3%AE-fe-2-o-3-%C3%AE-al-2-o-3-heterostructure-interface
#5
Y Wang, Z Tao, J Yu, X Zhang
The misfit dislocations at α-Fe2 O3 /α-Al2 O3 heterostructure interfaces were investigated by high-resolution transmission electron microscopy (HRTEM), geometric phase analysis (GPA) and dislocation density tensor analysis. When imaged along the [112¯0] direction, the misfit dislocation core is a mixed-type, which can be characterised by one extra (11¯02) plane and one extra (1¯104) plane of α-Al2 O3 . Dislocation density tensor analysis gave a very high accuracy in determining the corresponding Burgers vectors of two extra half-planes...
May 4, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29722438/influence-of-cnts-decomposition-during-reactive-friction-stir-processing-of-an-al-mg-alloy-on-the-correlation-between-microstructural-characteristics-and-microtextural-components
#6
F Khodabakhshi, M Nosko, A P Gerlich
Multipass friction-stir processing was employed to uniformly disperse multiwalled carbon nanotubes (MW-CNTs) within an Al-Mg alloy metal matrix. Decomposition of MW-CNTs occurs in situ as a result of solid-state chemical reactions, forming fullerene (C60) and aluminium carbide (Al4 C3 ) phases during reactive high temperature severe plastic processing. The effects of this decomposition on the microstructural features, dynamic restoration mechanisms and crystallographic microtextural developments are studied for the first time by using electron backscatter diffraction (EBSD) and transmission electron microscopy (TEM) analysis...
May 3, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29702728/collagen-iv-and-laminin-1-expression-in-embryonic-mouse-lens-using-principal-components-analysis-technique
#7
O Sijilmassi, J M López Alonso, M C Barrio Asensio, A Del Río Sevilla
Immunohistochemistry section staining is not always easy to interpret. Manual quantification of immunohistochemical staining is limited by the observer visual ability to detect changes in level staining. Hence, the quantification of immunostaining by means of digital image analysis allows us to measure accurately protein expression percentages in immunobiological stained tissues and ensures to overcome the visual limitations. We perform an experimental study to analyse the impact of folic acid (FA) deficiency into collagen IV and laminin-1 expression in the embryonic mouse lens...
April 27, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29698565/automated-detection-of-fluorescent-cells-in-in-resin-fluorescence-sections-for-integrated-light-and-electron-microscopy
#8
J Delpiano, L Pizarro, C J Peddie, M L Jones, L D Griffin, L M Collinson
Integrated array tomography combines fluorescence and electron imaging of ultrathin sections in one microscope, and enables accurate high-resolution correlation of fluorescent proteins to cell organelles and membranes. Large numbers of serial sections can be imaged sequentially to produce aligned volumes from both imaging modalities, thus producing enormous amounts of data that must be handled and processed using novel techniques. Here, we present a scheme for automated detection of fluorescent cells within thin resin sections, which could then be used to drive automated electron image acquisition from target regions via 'smart tracking'...
April 26, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29683482/parametric-investigations-on-the-saturation-intensity-of-coumarin-102-for-stimulated-emission-depletion-application
#9
H-Y Qin, W-X Zhao, W Zhao, C Zhang, X-Q Feng, S-P Liu, K-G Wang
Stimulated emission depletion (STED) microscopy performed using continuous-wave (CW) lasers has been investigated and developed by Willig et al. (Nature Methods, 2007, 4(11):915) for nearly a decade. Kuang et al. (Review of Scientific Instruments, 2010, 81:053709) developed the CW STED microscopy technique with 405 nm excitation and 532 nm depletion beams. In their research, Coumarin 102 dye was adopted and was found to be depletable. In this study, a parametric investigation of the depletion of Coumarin 102 dye is carried out experimentally...
April 23, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29676795/application-of-a-reflective-microscope-objective-for-multiphoton-microscopy
#10
Mohammad M Kabir, Aakash M Choubal, Kimani C Toussaint
Reflective objectives (ROs) mitigate chromatic aberration across a broad wavelength range. Yet, a systematic performance characterisation of ROs has not been done. In this paper, we compare the performance of a 0.5 numerical-aperture (NA) reflective objective (RO) with a 0.55 NA standard glass objective (SO), using two-photon fluorescence (TPF) and second-harmonic generation (SHG). For experiments spanning ∼1 octave in the visible and NIR wavelengths, the SO leads to defocusing errors of 25-40% for TPF images of subdiffraction fluorescent beads and 10-12% for SHG images of collagen fibres...
April 20, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29676794/classify-epithelium-stroma-in-histopathological-images-based-on-deep-transferable-network
#11
X Yu, H Zheng, C Liu, Y Huang, X Ding
Recently, the deep learning methods have received more attention in histopathological image analysis. However, the traditional deep learning methods assume that training data and test data have the same distributions, which causes certain limitations in real-world histopathological applications. However, it is costly to recollect a large amount of labeled histology data to train a new neural network for each specified image acquisition procedure even for similar tasks. In this paper, an unsupervised domain adaptation is introduced into a typical deep convolutional neural network (CNN) model to mitigate the repeating of the labels...
April 20, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29676793/massively-parallel-approximate-bayesian-computation-for-estimating-nanoparticle-diffusion-coefficients-sizes-and-concentrations-using-confocal-laser-scanning-microscopy
#12
M Röding, M Billeter
We implement a massively parallel population Monte Carlo approximate Bayesian computation (PMC-ABC) method for estimating diffusion coefficients, sizes and concentrations of diffusing nanoparticles in liquid suspension using confocal laser scanning microscopy and particle tracking. The method is based on the joint probability distribution of diffusion coefficients and the time spent by a particle inside a detection region where particles are tracked. We present freely available central processing unit (CPU) and graphics processing unit (GPU) versions of the analysis software, and we apply the method to characterize mono- and bidisperse samples of fluorescent polystyrene beads...
April 20, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29676789/electron-backscattered-diffraction-analysis-of-friction-stir-processed-nanocomposites-produced-via-spark-plasma-sintering
#13
B Sadeghi, P Cavaliere, M Shamanian, M Sanayei, J A Szpunar, M Nosko
In the present study, Spark Plasma Sintered (SPSed) aluminium matrix composites were severely deformed through Friction stir processing (FSP). Pure aluminium powders and bimodal sized Al2 O3 particles (80 nm and 25 μm) were firstly mixed by ball milling and then consolidated by spark plasma sintering. The effect of the heat input as well the bimodal particle size of the alumina on the materials' microstructure and texture development was evaluated by electron back scattered diffraction (EBSD) analysis. The EBSD analysis clearly showed that the SPSed nanocomposites possessed bimodal aluminium matrix grain structure as well as a crystallography characterised by random texture...
April 20, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29655273/a-review-of-techniques-for-visualising-soft-tissue-microstructure-deformation-and-quantifying-strain-ex-vivo
#14
C M Disney, P D Lee, J A Hoyland, M J Sherratt, B K Bay
Many biological tissues have a complex hierarchical structure allowing them to function under demanding physiological loading conditions. Structural changes caused by ageing or disease can lead to loss of mechanical function. Therefore, it is necessary to characterise tissue structure to understand normal tissue function and the progression of disease. Ideally intact native tissues should be imaged in 3D and under physiological loading conditions. The current published in situ imaging methodologies demonstrate a compromise between imaging limitations and maintaining the samples native mechanical function...
April 14, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29655172/sparse-coding-denoising-applied-to-reversible-conformational-switching-of-a-porphyrin-self-assembled-monolayer-induced-by-scanning-tunnelling-microscopy
#15
J Oliveira, A M Bragança, L Alcácer, J Morgado, M Figueiredo, J Bioucas-Dias, Q Ferreira
Scanning tunnelling microscopy (STM) was used to induce conformational molecular switching on a self-assembled monolayer of zinc-octaethylporphyrin on a graphite/tetradecane interface at room temperature. A reversible conformational change controlled by applying a tip voltage was observed. Consecutive STM images acquired at alternating tip voltages showed that at 0.4 V the porphyrin monolayer presents a molecular arrangement formed by alternate rows with two different types of structural conformations and when the potential is increased to 0...
April 14, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29630741/comparison-of-hyperspectral-classification-methods-for-the-analysis-of-cerium-oxide-nanoparticles-in-histological-and-aqueous-samples
#16
M P S Idelchik, J Dillon, L Abariute, M A Guttenberg, A Segarceanu, N M Neu-Baker, S A Brenner
Hyperspectral imaging (HSI) and classification are established methods that are being applied in new ways to the analysis of nanoscale materials in a variety of matrices. Typically, enhanced darkfield microscopy (EDFM)-based HSI data (also known as image datacubes) are collected in the wavelength range of 400-1000 nm for each pixel in a datacube. Utilising different spectral library (SL) creation methods, spectra from pixels in the datacube corresponding to known materials can be collected into reference spectral libraries (RSLs), which can be used to classify materials in datacubes of experimental samples using existing classification algorithms...
April 6, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29608216/comparisons-of-the-topographic-characteristics-and-electrical-charge-distributions-among-babesia-infected-erythrocytes-and-extraerythrocytic-merozoites-using-afm
#17
L Scudiero, W DE J Mercado-Rojano, A Rudolph, J Wang, J M Laughery, C E Suarez
Tick-borne Babesia parasites are responsible for costly diseases worldwide. Improved control and prevention tools are urgently needed, but development of such tools is limited by numerous gaps in knowledge of the parasite-host relationships. We hereby used atomic force microscopy (AFM) and frequency-modulated Kelvin probe potential microscopy (FM-KPFM) techniques to compare size, texture, roughness and surface potential of normal and infected Babesia bovis, B. bigemina and B. caballi erythrocytes to better understand the physical properties of these parasites...
April 2, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29603229/hrtemfringeanalyzer-a-free-python-module-for-an-automated-analysis-of-fringe-pattern-in-transmission-electron-micrographs
#18
Ivo Alxneit
A python module (HRTEMFringeAnalyzer) is reported to evaluate the local crystallinity of samples from high-resolution transmission electron microscopy images in a mostly automated fashion. The user only selects the size of a square analyser window and a step size which translates the window in the micrograph. Together they define the resolution of the results obtained. Regions where fringe patterns are visible are identified and their lattice spacing d and direction ϕ as well as the corresponding mean errors σ determined...
March 30, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29600565/recent-research-on-stimulated-emission-depletion-microscopy-for-reducing-photobleaching
#19
REVIEW
C Li, S Liu, W Wang, W Liu, C Kuang, X Liu
Stimulated emission depletion (STED) microscopy is a useful tool in investigation for super-resolution realm. By silencing the peripheral fluorophores of the excited spot, leaving only the very centre zone vigorous for fluorescence, the effective point spread function (PSF) could be immensely squeezed and subcellular structures, such as organelles, become discernable. Nevertheless, because of the low cross-section of stimulated emission and the short fluorescence lifetime, the depletion power density has to be extremely higher than the excitation power density and molecules are exposed in high risk of photobleaching...
March 30, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29574724/extremely-thin-layer-plastification-for-focused-ion-beam-scanning-electron-microscopy-an-improved-method-to-study-cell-surfaces-and-organelles-of-cultured-cells
#20
E G VAN Donselaar, B Dorresteijn, D Popov-Čeleketić, W J VAN DE Wetering, T C Verrips, T Boekhout, C T W M Schneijdenberg, A T Xenaki, T P VAN DER Krift, W H Müller
Since the recent boost in the usage of electron microscopy in life-science research, there is a great need for new methods. Recently minimal resin embedding methods have been successfully introduced in the sample preparation for focused-ion beam scanning electron microscopy (FIB-SEM). In these methods several possibilities are given to remove as much resin as possible from the surface of cultured cells or multicellular organisms. Here we introduce an alternative way in the minimal resin embedding method to remove excess of resin from two widely different cell types by the use of Mascotte filter paper...
March 25, 2018: Journal of Microscopy
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