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Journal of Chromatographic Science

Robert Zakrzewski, Pawel Urbaniak, Arkadiusz Nowicki, Waldemar Tejchman
Hydrophobicity of the eight 2-thioxo-1,3-thiazolidin-4-one derivatives was determined experimentally by thin-layer chromatography and predicted by means of commercially available programmers. RM values were determined by reversed-phase thin-layer chromatography with using acetonitrile-water, methanol-water, acetone-water, propan-2-ol-water or 1,4-dioxane-water and compared with logP values calculated by using computer programs: HyperChem 8.0.10, Virtual Chemical Calculation Laboratory, ACD/LogP. The drug-likeness has been calculated using Molinspiration...
May 18, 2018: Journal of Chromatographic Science
Qian Shao, Lin Chang, Zhenya Wei, Yun Wei
Hyperoside, 3'-O-methylquercetin 3-O-β-D-galactopyranoside, astragalin and 3'-O-methylquercetin 3-O-β-D-glucopyranoside from an invasive weed Solanum rostratum Dunal were separated and purified successfully by high-speed counter-current chromatography (HSCCC) with a solvent system composed of n-hexane-ethyl acetate-methanol-water (1:7:1:7, v/v) and gradient elution mode preparative high-performance liquid chromatography (prep-HPLC) with low column temperature. In the sample pretreatment section, target compounds in aqueous extract of the weed were concentrated using solvent sublation...
May 16, 2018: Journal of Chromatographic Science
Shubo Dong, Hanyue Yang, Deju Ye
A rapid and sensitive high-performance liquid chromatography-mass spectrometry method was developed and validated to determine 4-fluoroaniline concentration in ezetimibe. Chromatographic separation was achieved on a Phenomenex Gemini-NX C18 column (150 × 4.6 mm, 3 μm) maintained at 30°C. The liquid chromatography system was operated in gradient mode with an injection volume of 20 μL at a flow rate of 1 mL/min. Mobile phase A was water and mobile phase B consisted of acetonitrile with 0.05% acetic acid. The detection was performed using a single quadrupole mass spectrometer in single ion monitoring mode by using positive ionization...
May 14, 2018: Journal of Chromatographic Science
Anggy Lusanna Gutiérrez Ortiz, Federico Berti, Luciano Navarini, Paola Crisafulli, Silvia Colomban, Cristina Forzato
Identification of both hydroxycinnamic and chlorogenic acids present in aqueous extracts of walnut leaves (Juglans regia L.) were carried out by using, for the first time, standard compounds not commercially available for qualitative identification. In particular, in addition to caffeic, ferulic, p-coumaric and sinapic acids, cis and trans mono-caffeoylquinic, dicaffeoylquinic, mono-feruloylquinic and cis and trans mono-p-coumaroylquinic acid isomers were detected and quantified by Ultra High Pressure Liquid Chromatography and the seasonal variations of these secondary metabolites were investigated...
May 14, 2018: Journal of Chromatographic Science
Negin Farshchi, Ali Abbasian, Kambiz Larijani
Limonene is a colorless liquid hydrocarbon and had been investigated as a plasticizer for many plastics. Prediction of solubility between different materials is an advantage in many ways, one of the most convenient ways to know the compatibility of materials is to determine the degree of solubility of them in each other. The concept of "solubility parameter" can help practitioners in this way.In this study, inverse gas chromatography (IGC) method at infinite dilution was used for determination of the thermodynamic properties of DL-p-mentha-1,8-diene, 4-Isopropyl-1-methylcyclohexene (DL-limonene)...
May 10, 2018: Journal of Chromatographic Science
Nourah Zoman Alzoman, Hadir Mohamed Maher, Hessa Al-Showiman, Ghada Ahmed Fawzy, Areej Mohammad Al-Taweel, Shagufta Perveen, Rasool Bakhsh Tareen, Ruba Mahmoud Al-Sabbagh
A precise, accurate, selective and sensitive capillary electrophoresis method using a diode array detector was developed for the first time for the determination of both scutellarein (SLN) and caffeic acid (CAA) in prepared Abelia triflora extract. Electrophoretic analysis was performed using a background electrolyte solution consisting of borax buffer (40 mM, pH 9.2) and a 200-nm detection wavelength. This method was fully validated according to The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines...
May 10, 2018: Journal of Chromatographic Science
Yuji Mano
A bioanalytical method for the simultaneous determination of oxcarbazepine (OXC) and its pharmacologically active metabolite, 10, 11-dihydro-10-hydroxycarbamazepine (HOXC), in human plasma was developed using a high-performance liquid chromatography with tandem mass spectrometry. After protein precipitation by acetonitrile, the analytes (OXC and HOXC) and a stable-labeled isotope of OXC as an internal standard (IS) were chromatographed on a Synergi Hydro-RP column (2.0 mm × 50 mm, 4 μm) with a gradient elution at a flow rate 0...
May 4, 2018: Journal of Chromatographic Science
Jie-Hua Shi, Li-Hua Zhao, Guo-Feng Shen
A poly(Methacrylate-co-Ethyleneglycol dimethacrylate) monolithic column was prepared in situ for high performance liquid chromatography and characterized utilizing scanning electron microscopy, thermodynamic parameters and linear solvation energy relationship methodology. The results revealed that there were many microglobules forming the larger cluster in poly(Methacrylate-co-Ethyleneglycol dimethacrylate) monolith prepared under the selected preparation conditions, which composed continuously aligned macroporous channels through the monolith skeleton...
April 27, 2018: Journal of Chromatographic Science
Larissa Stebounova, Scott M Ebert, Logan T Murry, Christopher M Adams, Daryl J Murry
Ultra-performance liquid chromatography (UPLC) interfaced with atmospheric pressure chemical ionization mass-spectrometry was used to separate and quantify ursolic acid (UA) and oleanolic acid (OA) in human plasma. UA and OA were extracted from 0.5 mL human plasma using supported liquid extraction and separated utilizing an Acquity UPLC HSS column. The method has been validated for both UA and OA quantitation with a limit of detection of 0.5 ng/mL. The UPLC separations are carried out with isocratic elution with methanol and 5 mM ammonium acetate in water (85:15) as a mobile phase at a flow rate of 0...
April 26, 2018: Journal of Chromatographic Science
Wenli Hui, Qiuhui Li, Huiya Ma, Mengdi Wu, Kun Feng, Hongli Zhu, Pengfei Yang, Jun Li, Chao Chen, Kunping Yan
A rapid and simple high-performance liquid chromatography-ultraviolet method was developed for the separation and quantification of 15 sulfonamides (SAs) in foods of animal origin without the need of clean-up procedure. A mixture of acetonitrile-formic acid-ammonium acetate-water was used as the mobile phase to separate 15 SAs on a C18 column with gradient. The selected SAs were separated completely from the matrix mixture based on different retention behaviors at different concentration of acetonitrile. The effects of the additive of formic acid and ammonium acetate in mobile phases on the separation of SAs were also investigated...
April 26, 2018: Journal of Chromatographic Science
Xuan Zeng, Weiwei Su, Hong Liu, Yuying Zheng, Taobin Chen, Weijian Zhang, Zenghao Yan, Yang Bai, Hongliang Yao
A rapid resolution liquid chromatography tandem mass spectrometry method was developed and validated for simultaneous determination of rosuvastatin, naringin and naringenin in rat plasma. Chromatographic separation of analytes and internal standard (fluvastatin for rosuvastatin, while isoquercitrin for naringin and naringenin) was performed on Agilent Poroshell 120 EC-C18 column (3.0 × 50 mm, 2.7 μm) using gradient elution with a mobile phase of methanol and water, both with 0.1% formic acid (v/v). The detection was operated in multiple reaction monitoring mode to monitor the precursor-to-product ion transitions of m/z 579...
April 26, 2018: Journal of Chromatographic Science
Abubakar Lawal, Richard Chee Seng Wong, Guan Huat Tan, Lukman Bola Abdulra'uf, Ali Mohamed Ali Alsharif
Fruits and vegetables constitute a major type of food consumed daily apart from whole grains. Unfortunately, the residual deposits of pesticides in these products are becoming a major health concern for human consumption. Consequently, the outcome of the long-term accumulation of pesticide residues has posed many health issues to both humans and animals in the environment. However, the residues have previously been determined using conventionally known techniques, which include liquid-liquid extraction, solid-phase extraction (SPE) and the recently used liquid-phase microextraction techniques...
April 21, 2018: Journal of Chromatographic Science
Ruofeng Shang, Chao Zhang, Yunpeng Yi, Yu Liu, Wanxia Pu
A simple, sensitive and reproducible high-performance liquid chromatography method was developed and validated for the determination of 14-O-[(2-amino-1,3,4-thiadiazol-5-yl) thioacetyl] mutilin (ATTM), a new synthesized pleuromutilin derivative with potent antibacterial activity, in broiler chicken plasma after a single intravenous (i.v.), intramuscular (i.m.) or oral (p.o.) administration. Satisfactory separation was achieved on a ZORBAX Ecliplus C18 column (250 × 4.6, 5 μm) with UV detection at 279 nm, using a mobile phase comprising acetonitrile and ultrapure water (50:50, v/v)...
April 13, 2018: Journal of Chromatographic Science
Man Liao, Xiaoye Cheng, Xia Zhang, Xinpeng Diao, Caijuan Liang, Lantong Zhang
Trollius ledebourii has been more involved in Mongolian medicine and is often used as a type of tea for heat-clearing and detoxifying in the populus. In this study, a rapid and sensitive method was established for the qualitative and quantitative analyses of the major constituents in T. ledebourii. Ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry was developed for the identification of the multi-constituents in T. ledebourii. A total of 37 chemical constituents in T...
April 13, 2018: Journal of Chromatographic Science
Xia Zhang, Caijuan Liang, Changlin Li, Mingde Bu, Liying Bu, Yuande Xiao, Hongguang Sun, Lantong Zhang
This study reported the identification and determination of the main components of Commelina communis Linn. A total of 62 compounds were identified in C. communis Linn. extract, which included 29 flavonoids and flavonoid glycosides, 17 phenolic acids, 4 alkaloids, 1 pyrimidine alkaloids, 3 sterols and 8 fatty acids and others by ultra high performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry. Moreover, a specific, simple, rapid and sensitive high performance liquid chromatography with tandem mass spectrometry method was developed and validated for determination of 13 components of C...
April 11, 2018: Journal of Chromatographic Science
Bin Zheng, Hongmei Hu, Xiaoning Zhang, Yuanming Guo, Xiaojun Zhang, Lijian Xue, Xingwei Xiang, Lu Zhang
A simple, rapid and novel method has been developed and validated for the determination of seven indicator polychlorinated biphenyls (PCBs) in seafood samples by gas chromatography coupled to electron capture detector. Freeze-dried samples were done first ultrasonic extraction by n-hexane:methylene chloride:acetone (3:1:1, v/v), and then one-step clean-up (dispersive solid-phase extraction clean-up) or two-step clean-up (concentrated sulfuric acid purification and dispersive solid-phase extraction clean-up) was selected according to the lipid contents of the samples, if the lipid content was no more than 1%, one-step clean-up was used, otherwise, two-step clean-up was chose...
April 11, 2018: Journal of Chromatographic Science
Feng Li, Hong Wu, Liang-Liang Sun, Huan Wu, Rong Wang, Shu-Ping Li, Wen-Yu Wang, Li Dai, Zheng-Rong Zhang, Jun Fu, Ran Deng
A simple and effective method of high performance liquid chromatography (HPLC) with diode array detection was established to identify the origin of Achyranthes bidentata Blume and evaluate its quality, based on chromatographic fingerprint combined with the similarity analysis, hierarchical cluster analysis and the quantitative analysis of multi-components by single marker (QAMS). In the chromatographic fingerprint, 16 peaks were selected as the common model to evaluate the similarities among 18 batches (S1-S18) of A...
April 11, 2018: Journal of Chromatographic Science
Rafael Henrique Dias Reis, Fávero Reisdorfer Paula, Michel Mansur Machado, Jonathaline Apollo Duarte, Luís Flávio Souza de Oliveira, Clésio Soldateli Paim, Marcelo Donadel Malesuik
Stability studies of the pharmaceutically important compound finasteride were conducted in order to evaluate decomposition of the drug under forced degradation conditions. A simple stability-indicating liquid chromatography method was developed and validated for the evaluation of finasteride and degradation products formed in pharmaceutical preparations and the raw material. Isocratic LC separation was achieved on a C18 column using a mobile phase of o-phosphoric acid (0.1% v/v), adjusted to pH 2.8 with triethylamine (10% v/v) and acetonitrile (52:48 v/v), with a flow rate of 1...
April 9, 2018: Journal of Chromatographic Science
Magda M Ayad, F Belal, Mervet M Hosney, Heba Elmansi, Nora Elsayed
The proposed method describes a high performance liquid chromatographic method with fluoremetric detection for the determination of cisatracurium (CIS) and propofol (PRP) simultaneously, which are co-administered as a pre-operative injection mixture. The separation of the two drugs was achieved using monolithic column (100 mm and 4.6 mm internal diameter) and mixture of methanol and 0.1 M phosphate buffer in the ratio of 80:20 (v/v) at pH 4.5 as a mobile phase. The fluorescence detection was carried out at 230/324 nm...
April 9, 2018: Journal of Chromatographic Science
Igor I Miroshnichenko, Natalia V Baymeeva
A quantitative method was developed to support therapeutic drug monitoring of eight antipsychotic drugs: chlorpromazine, haloperidol, zuclopenthixol, clozapine, risperidone, quetiapine, aripiprazole or olanzapine and some active metabolites (dehydroaripiprazole, N-desmethylclozapine and 9-hydroxyrisperidone) in human serum. Separation of the compounds was achieved using a Zorbax SB-C18 (150 mm × 4.6 mm, 5 μm) column and mass-spectrometric detection in multiple reaction monitoring mode. Human blood samples were collected in vacutainer tubes and the analytes were extracted with methyl-tert-butyl ether...
April 7, 2018: Journal of Chromatographic Science
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