journal
MENU ▼
Read by QxMD icon Read
search

Journal of General and Applied Microbiology

journal
https://www.readbyqxmd.com/read/28502957/1-methoxypyrrole-2-carboxamide-a-new-pyrrole-compound-isolated-from-streptomyces-griseocarneus-sww368
#1
Watsapon Wattanasuepsin, Bungonsiri Intra, Jirayut Euanorasetr, Yoshihiro Watanabe, Ratchanee Mingma, Wataru Fukasawa, Mihoko Mori, Atsuko Matsumoto, Kazuro Shiomi, Watanalai Panbangred
A new pyrrole compound, 1-methoxypyrrole-2-carboxamide, was obtained from a culture broth of Streptomyces griseocarneus SWW368, which was isolated from the rhizospheric soil under a Para rubber tree (Hevea brasiliensis). The chemical structure was elucidated by 1D NMR, 2D NMR, and MS, as a pyrrole ring with a N-methoxy group and a primary amide group. It exhibited antibacterial properties against Kocuria rhizophila, Staphylococcus aureus and Xanthomonas campestris pv. oryzae; however, cytotoxicity of the compound at 714 μM against several mammalian tumor cell lines, i...
May 15, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28502956/evaluation-of-biofilm-forming-ability-of-bacterial-strains-isolated-from-the-roof-of-an-old-house
#2
Marwa Jardak, Faten Abdelli, Rahma Laadhar, Raphael Lami, Didier Stien, Sami Aifa, Sami Mnif
The bacterial diversity associated with biofilm-forming ability was studied. Eighteen bacterial strains were isolated from a microbial film collected from the roof of an old house located in Sfax, Tunisia. The purity of these microorganisms was confirmed by microscopic observation after repeated streaking on a Tryptic Soy agar medium. Biofilm formation was estimated using preliminary tests including a motility test, microbial adhesion to solvents (MATS), and the Congo Red Agar method (CRA). Since these tests showed no significant result, microplate tests, such as crystal violet and resazurin assays, were used...
May 15, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28502955/blue-light-inhibits-the-enlargement-of-erythrobacter-litoralis-spheroplasts
#3
Koki Nishino, Hiromi Nishida
No abstract text is available yet for this article.
May 15, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28484117/highly-efficient-liposome-mediated-transformation-system-for-the-basidiomycetous-fungus-flammulina-velutipes
#4
Liang Shi, Dongdong Chen, Chao Xu, Ang Ren, Hanshou Yu, Mingwen Zhao
Flammulina velutipes is a well-known edible mushroom cultivated all over the world. However, because of the low transformation frequency, the expensive instruments required, and the complicated, time-consuming procedures necessary, there is insufficient genetic research on F. velutipes. In this study, we report a liposome-mediated transformation (LMT) system for the genetic transformation of F. velutipes. Using the LMT system, we obtained 82 ± 4 stable F. velutipes transformants per 10(5) protoplasts, which is a clear increase in transformation frequency compared to the other methods used...
May 2, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28484116/highly-efficient-gene-targeting-in-aspergillus-oryzae-industrial-strains-under-ligd-mutation-introduced-by-genome-editing-strain-specific-differences-in-the-effects-of-deleting-ecdr-the-negative-regulator-of-sclerotia-formation
#5
Hidetoshi Nakamura, Takuya Katayama, Tomoya Okabe, Kazuhiro Iwashita, Wataru Fujii, Katsuhiko Kitamoto, Jun-Ichi Maruyama
Numerous strains of Aspergillus oryzae are industrially used for Japanese traditional fermentation and for the production of enzymes and heterologous proteins. In A. oryzae, deletion of the ku70 or ligD genes involved in non-homologous end joining (NHEJ) has allowed high gene targeting efficiency. However, this strategy has been mainly applied under the genetic background of the A. oryzae wild strain RIB40, and it would be laborious to delete the NHEJ genes in many A. oryzae industrial strains, probably due to their low gene targeting efficiency...
May 2, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28484115/microbially-reduced-graphene-oxide-shows-efficient-electricity-ecovery-from-artificial-dialysis-wastewater
#6
Yuko Goto, Naoko Yoshida
Anodes are crucial in determining the electricity recovery of microbial fuel cells (MFCs). In this study, graphene oxide (GO) was used as an anodic material for electricity recovery from artificial dialysis wastewater (ADWW). Anaerobic incubation of ADWW with GO for 21 days produced a hydrogel complex containing embedded microbial cells and microbially reduced GO (rGO). The rGO complex recovered 540 to 810 μA/cm(3) of catalytic current from ADWW after 10 days of electrochemical cultivation at 200 mV (vs. Ag/AgCl), which was approximately thirty times higher than that recovered from graphite felt (GF), a representative anode in MFCs...
May 2, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28392542/new-plasmids-for-the-disruption-and-repeated-use-of-selection-markers-in-saccharomyces-cerevisiae
#7
Kenshiro Nihei, Tsutomu Kishi
No abstract text is available yet for this article.
April 7, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28392541/analysis-of-strain-specific-genes-in-glutamic-acid-producing-corynebacterium-glutamicum-ssp-lactofermentum-aj-1511
#8
Yousuke Nishio, Chie Koseki, Naoto Tonouchi, Kazuhiko Matsui, Shinichi Sugimoto, Yoshihiro Usuda
Strains of the bacterium, Corynebacterium glutamicum, are widely used for the industrial production of L-glutamic acid and various other substances. C. glutamicum ssp. lactofermentum AJ 1511, formerly classified as Brevibacterium lactofermentum, and the closely related C. glutamicum ATCC 13032 have been used as industrial strains for more than 50 years. We determined the whole genome sequence of C. glutamicum AJ 1511 and performed genome-wide comparative analysis with C. glutamicum ATCC 13032 to determine strain-specific genetic differences...
April 7, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28392540/isolation-of-streptococcus-gallolyticus-with-very-high-degradability-of-condensed-tannins-from-feces-of-the-wild-japanese-rock-ptarmigans-on-mt-tateyama
#9
Sayaka Tsuchida, Koichi Murata, Moriya Ohkuma, Kazunari Ushida
No abstract text is available yet for this article.
April 7, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28344193/polyphasic-insights-into-the-microbiomes-of-the-takamatsuzuka-tumulus-and-kitora-tumulus
#10
Junta Sugiyama, Tomohiko Kiyuna, Miyuki Nishijima, Kwang-Deuk An, Yuka Nagatsuka, Nozomi Tazato, Yutaka Handa, Junko Hata-Tomita, Yoshinori Sato, Rika Kigawa, Chie Sano
Microbial outbreaks and related biodeterioration problems have affected the 1300-year-old multicolor (polychrome) mural paintings of the special historic sites Takamatsuzuka Tumulus (TT) and Kitora Tumulus (KT). Those of TT are designated as a national treasure. The microbiomes of these tumuli, both located in Asuka village, Nara, Japan, are critically reviewed as the central subject of this report. Using culture-dependent methods (conventional isolation and cultivation), we conducted polyphasic studies of the these microbial communities and identified the major microbial colonizers (Fusarium spp...
March 24, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28331163/in-vitro-evaluation-of-candidate-bacillus-spp-for-animal-feed
#11
Sirima Mingmongkolchai, Watanalai Panbangred
The aim of this study was to select aerobic spore-formers for animal feed based on their in vitro probiotic potential, including their enzyme-producing ability and safety assessment. Seven isolates out of 187 spore-forming bacteria were selected for their ability to produce cellulase (89.21-1668.32 U/ml), xylanase (1399.68-4351.10 U/ml), and phytase (2.72-28.70 U/ml). Among seven isolates, five had activities towards a broad range of p-nitrophenyl esters with acyl chain lengths from C2 to C12. The probiotic properties of all selected isolates varied with respect to their acid and bile salt tolerance under simulated gastrointestinal tract (GIT) conditions, and their adherence ability to human intestinal cell lines (Caco-2 and HT-29)...
March 22, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28331162/carbon-and-nitrogen-depletion-induced-nucleophagy-and-selective-autophagic-sequestration-of-a-whole-nucleus-in-multinucleate-cells-of-the-filamentous-fungus-aspergillus-oryzae
#12
Takashi Kikuma, Takahiro Mitani, Takahiro Kohara, Jun-Ichi Maruyama, Katsuhiko Kitamoto
Autophagy is a conserved cellular degradation process in eukaryotes, in which cytoplasmic components and organelles are digested in vacuoles/lysosomes. Recently, autophagic degradation of nuclear materials, termed "nucleophagy", has been reported. In the multinucleate filamentous fungus Aspergillus oryzae, a whole nucleus is degraded by nucleophagy after prolonged culture. While developing an H2B-EGFP processing assay for the evaluation of nucleophagy in A. oryzae, we found that nucleophagy is efficiently induced by carbon or nitrogen depletion...
March 22, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28331161/isolation-of-cyanobacterial-mutants-exhibiting-growth-defects-under-microoxic-conditions-by-transposon-tagging-mutagenesis-of-synechocystis-sp-pcc-6803
#13
Kazuki Terauchi, Riho Sobue, Yuho Furutani, Rina Aoki, Yuichi Fujita
Cyanobacteria are photosynthetic prokaryotes that perform oxygenic photosynthesis by extracting electrons from water, with the generation of oxygen as a byproduct. Cyanobacteria use oxygen not only for respiration to produce energy in the dark but also for biosynthesis of various metabolites, such as heme and chlorophyll. Oxygen levels dynamically fluctuate in the field environments, from hyperoxic at daytime to almost anaerobic at night. Thus, adaptation to anaerobiosis should be important for cyanobacteria to survive in low-oxygen and anaerobic environments...
March 22, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28154339/construction-of-a-photo-responsive-chimeric-histidine-kinase-in-escherichia-coli
#14
Mayuko Hori, Shyunsuke Oka, Yoshimi Sugie, Hokuto Ohtsuka, Hirofumi Aiba
Two-component signal transduction systems (TCS), that are also referred to as His to Asp phosphorelay systems, are involved in widespread cellular responses to diverse signals from bacteria to plants. Previously, we succeeded in reconstructing a cyanobacterial photo-perception system in Escherichia coli by employing a CcaS-CcaR two-component system from Nostoc punctiforme. In this study, we have added a photo-responsive ability to ArcB-ArcA (anoxic redox control) TCS of E. coli by fusing a cyanobacterial photoreceptor domain of CcaS with an intracellular histidine kinase (HK) domain of ArcB...
March 17, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28123132/purification-and-characterization-of-a-novel-extracellular-neutral-metalloprotease-from-cerrena-albocinnamomea
#15
Shigeki Hamada, Keisuke Kubota, Masanobu Sagisaka
We selected a fungus secreting a neutral protease from soil and identified it as the basidiomycete fungus Cerrena albocinnamomea according to its ITS-5.8S rDNA and 28S rDNA-D1/D2 sequences. A major extracellular protease isolated from C. albocinnamomea was purified approximately 44-fold through two purification steps. SDS-PAGE analyses of the purified protease revealed a single band, and its molecular mass of 39,756 Da was determined using MALDI-TOF-MS. The enzyme was optimally active at approximately pH 7...
March 17, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28123131/silver-nanoparticles-as-an-antimicrobial-agent-a-case-study-on-staphylococcus-aureus-and-escherichia-coli-as-models-for-gram-positive-and-gram-negative-bacteria
#16
Eman Zakaria Gomaa
The current research was focused on the characterization and antimicrobial activity of silver nanoparticles (AgNPs) produced by Bacillus licheniformis NM120-17. The synthesis was initially observed by a colour change from pale yellow to brown which was further confirmed by UV-Vis spectroscopy. The AgNPs were characterized using TEM, EDAX and FTIR. The synthesized nanoparticles were found to be spherical and uniformly distributed with a size in the range of 9-27.5 nm. The antibacterial activities and acting mechanism of AgNPs were studied with respect to Staphylococcus aureus and Escherichia coli by measuring the growth curves, protein and reducing sugar leakage, respiratory chain dehydrogenase activity, as well as the formation of bactericidal reactive oxygen species (ROS)...
March 17, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/27990001/archaeal-histone-distribution-is-associated-with-archaeal-genome-base-composition
#17
Hiromi Nishida, Taku Oshima
A subpopulation of Archaea possesses histones, which are similar to eukaryotic histones H3 and H4. However, archaeal histones are smaller than H3 and H4, and are not post-translationally modified. In addition, the fundamental unit of archaeal histones might be a dimer. The organization of archaeal nucleosomes, therefore, differs from that of eukaryotic nucleosomes. The base compositions of archaeal genome are much more diversified than those of eukaryote and the archaeal histones have more diversified amino acid sequences, which are reflected by their varied isoelectric points...
March 17, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/27990000/phenotypic-difference-between-%C3%AE-srl-reca-306-and-%C3%AE-reca-km-elucidated-by-next-generation-sequencing-combined-with-a-long-pcr-system
#18
COMPARATIVE STUDY
Shingo Suzuki, Akihiro Kaidow, Takuma Meya, Anri Masuya, Takashi Shiina
Many significant gene mutations in E. coli have contributed to the development of genetics. Among these, a commonly used recA mutation, Δ(srl-recA)306 has been sequenced by a next-generation sequencer combined with a long PCR. An original report described that Δ(srl-recA)306 cells were deleted from srlR to recA genes in their genome. The next-generation sequencer enables more accurate details to be determined. We ask whether both surrounding genes from hypF to norV for srlR and alaS for recA is there first...
March 17, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/27989999/candidate-nematicidal-proteins-in-a-new-pseudomonas-veronii-isolate-identified-by-its-antagonistic-properties-against-xiphinema-index
#19
Hayron Canchignia, Fabiola Altimira, Christian Montes, Evelyn Sánchez, Eduardo Tapia, María Miccono, Daniel Espinoza, Carlos Aguirre, Michael Seeger, Humberto Prieto
The nematode Xiphinema index affects grape vines and transmits important viruses associated with fanleaf degeneration. Pseudomonas spp. are an extensive bacterial group in which important biodegradation and/or biocontrol properties can occur for several strains in the group. The aim of this study was to identify new Pseudomonas isolates with antagonist activity against X. index. Forty bacterial isolates were obtained from soil and root samples from Chilean vineyards. Thirteen new fluorescent pseudomonads were found and assessed for their antagonistic capability...
March 17, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/27989998/phylogenetic-analysis-reveals-the-taxonomically-diverse-distribution-of-the-pseudomonas-putida-group
#20
Kenta Yonezuka, Jun Shimodaira, Michiro Tabata, Shoko Ohji, Akira Hosoyama, Daisuke Kasai, Atsushi Yamazoe, Nobuyuki Fujita, Takayuki Ezaki, Masao Fukuda
Pseudomonas putida is well-known for degradation activities for a variety of compounds and its infections have been reported. Thus, P. putida includes both clinical and nonclinical isolates. To date, no reports have examined the phylogenetic relationship between clinical and nonclinical isolates of the P. putida group. In this study, fifty-nine strains of P. putida group containing twenty-six clinical, and thirty-three nonclinical, isolates, were subjected to phylogenetic and taxonomic analyses based on 16S rRNA gene sequences and nine housekeeping gene sequences, including argS, dnaN, dnaQ, era, gltA, gyrB, ppnK, rpoB, and rpoD, to obtain insights into the diversity of species in this group...
March 17, 2017: Journal of General and Applied Microbiology
journal
journal
22022
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"