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Microvascular Research

Robin Mirdell, Aukje Nienke Lemstra-Idsardi, Simon Farnebo, Erik Tesselaar
OBJECTIVES: Laser speckle contrast imaging (LSCI) has seen limited use in the study of perfusion dynamics such as vasomotion. The aim of this study was to investigate the effects of a prolonged seated position on perfusion dynamics in the nail bed using LSCI. METHODS: Perfusion was recorded in digits II to IV bilaterally for 20 min during two separate sessions in ten healthy volunteers. The acclimatization period was 5 min for the 1st session and 20 min for the 2nd...
October 12, 2018: Microvascular Research
Wenbin Wang, Xingyang Zhu, Xiaolong Du, Aman Xu, Xiao Yuan, Yanqing Zhan, Mulin Liu, Shuanhu Wang
Venous thromboembolism (VTE), encompassing deep venous thrombosis (DVT) and pulmonary embolism (PE), is the third most common cardiovascular disease. miR-150 is one of important microRNAs which play critical role in various cellular function such as endothelial progenitor cells (EPCs). In this study, we investigate the effect of miR-150 on EPCs function ex vivo and thrombus resolution in vivo. We determined miR-150 expression in EPCs isolated from DVT patients and control subjects by RT-PCR. Potential target of miR-150 was confirmed by bioinformatics analysis and luciferase reporter respectively...
October 10, 2018: Microvascular Research
Anna Adamska, Aleksandra Araszkiewicz, Stanislaw Pilacinski, Agnieszka Gandecka, Agata Grzelka, Katarzyna Kowalska, Agnieszka Malinska, Michal Nowicki, Dorota Zozulinska-Ziolkiewicz
In patients with diabetes, functional changes in microcirculation and subclinical vascular pathology precede clinical manifestation of microangiopathic complications. The objective of this study was to evaluate the association between established vascular risk factors and density, maturity, and reactivity of dermal blood vessels in adults with type 1 diabetes (DM1). We included 148 DM1 patients (87 men) with a median (IQR) age of 40.5 (30.5-49) years and a median diabetes duration of 21 (17-29.5) years. The control group consisted of 13 healthy volunteers (6 men) with a median (IQR) age of 36 (31-43)...
October 9, 2018: Microvascular Research
Danilo Iannetta, Erin Calaine Inglis, Rogerio N Soares, Kaitlin M McLay, Silvia Pogliaghi, Juan M Murias
BACKGROUND: Cardiovascular disease (CVD) is associated with impairments in microvascular responsiveness. Therefore, reliably assessing microvascular function is clinically relevant. Thus, this study aimed to examine the reliability of the near-infrared spectroscopy (NIRS)-derived oxygen saturation (StO2 ) reperfusion slope, a measure of microvascular responsiveness, to four different vascular occlusion tests (VOT) of different durations in young and older participants. METHODS: Eight healthy young (29 ± 5 yr) and seven older (67 ± 4 yr) men participated in four NIRS combined with VOT (NIRS-VOT; 30 s, 1, 3, and 5 min) in the leg microvasculature on two visits separated by 1-2 weeks...
October 4, 2018: Microvascular Research
Jingjiang Xu, Yuandong Li, Shaozhen Song, William Cepurna, John Morrison, Ruikang K Wang
We report the development of a 1300 nm swept-source optical coherence tomography (SS-OCT) system specifically designed to perform OCT imaging and optical microangiography (OMAG) in rat eyes in vivo and its use in evaluating the effects of intraocular pressure (IOP) elevation on ocular circulation. The swept laser is operated in single longitude mode with a 90 nm bandwidth centered at 1300 nm and 200 kHz A-line rate, providing remarkable sensitivity fall-off performance along the imaging depth, a larger field of view of 2...
September 26, 2018: Microvascular Research
Elżbieta Grześk, Nasser Darwish, Wioleta Stolarek, Michał Wiciński, Bartosz Malinowski, Igor Burdziński, Grzegorz Grześk
BACKGROUND: Ischemia and reperfusion remain inseparable elements of numerous medical procedures such as by-pass surgery, organ transplantation or other cardiology and intervention radiology. The contraction of the smooth muscle of the vessel is considered to be one of the basic components leading to impaired perfusion, an increase in the oxygen deficit of the endothelium of the vessel, and subsequently also to tissues vascularized by the vessel. Main aim of this study was to evaluate the effect of ischemia and reperfusion on vascular smooth muscle cells stimulated pharmacologically with mastoparan-7 (direct G-protein activator) in comparison to stimulation of G-protein coupled receptor agonist - phenylephrine, and direct calcium channel activator - Bay K8644...
September 12, 2018: Microvascular Research
Sivasankar Shanmuganathan, Narayanasamy Angayarkanni
PURPOSE: Diabetic retinopathy (DR) is characterized by pro-inflammatory, pro-angiogenic and pro-fibrotic environment during the various stages of the disease progression. Basement membrane changes in the retina and formation of fibrovascular membrane are characteristically seen in DR. In the present study the effect of Alcoholic (AlE) extracts of Triphala an ayurvedic herbal formulation and its chief compounds, Chebulagic (CA), Chebulinic (CI) and Gallic acid (GA) were evaluated for TGFβ1-induced anti-fibrotic activity in choroid-retinal endothelial cells (RF/6A)...
September 4, 2018: Microvascular Research
Lin Deng, Hong Chen, Na Wei, Zhaodi Zhang, Guonian Wang
BACKGROUND: Dexmedetomidine (DEX) is an α2 -adrenergic receptor agonist commonly used during perioperative periods due to its sedation and analgesia effect. It is confirmed that DEX has cardioprotective effects against ischemia/reperfusion (I/R) injury. We investigated whether DEX administration is beneficial to type 2 diabetic rats subjected to I/R injury. METHODS: The diabetes model was established by providing a high-fat diet for 2 weeks followed by injecting 35 mg/kg streptozotocin (STZ)...
September 1, 2018: Microvascular Research
Rafi Sheikh, Josefine Bunke, Rannveig Linda Thorisdottir, Jenny Hult, Kajsa Tenland, Bodil Gesslein, Nina Reistad, Malin Malmsjö
BACKGROUND: The time taken for epinephrine to achieve its optimal effect during local anesthesia has recently become the subject of debate. The time from injection to commencement of surgery is traditionally quoted to be 7 to 10 min, while recent reports claim that it may take 30 min to achieve maximum hypoperfusion, which would prolong the time required for surgical procedures. The discrepancy may be related to difficulties associated with the techniques used to measure blood perfusion...
August 25, 2018: Microvascular Research
Haifeng Chen, Nan Lu, Changsheng Xu, Meifan Zheng
OBJECTIVE: To study the mechanism of heat shock protein 27 (HSP27) phosphorylation in the visfatin-induced proliferation and its protective effect against high-glucose induced apoptosis in HUVECs. METHODS: Human umbilical vascular endothelial cells (HUVECs) were isolated from umbilical veins and treated with visfatin. The proliferation of HUVECs was measured by the MTT assay and the apoptosis of HUVECs was detected by TUNEL assay. The phospho-HSP27 expression was detected by Western blot...
August 23, 2018: Microvascular Research
Karthikka Palanisamy, Ragavachetty Nagaraj Nareshkumar, Suganya Sivagurunathan, Rajiv Raman, Konerirajapuram Natarajan Sulochana, Subbulakshmi Chidambaram
Neovascularization in retina and choroid involves interplay of many cytokines and growth factors. Vascular endothelial growth factor (VEGF) being a pro-angiogenic molecule has been found to be high in aqueous and vitreous humour of patients with proliferative diabetic retinopathy (PDR). VEGF is also found in the fibroblast and retinal pigment epithelial cells (RPE) of choroidal neovascular (CNV) membranes isolated from patients. Though anti-VEGF agents cause regression of clinically visible new vessels, there is evidence that they increase the occurrence of retinal tractional detachment and other adverse effects in PDR and CNV treatments...
August 23, 2018: Microvascular Research
Desiree Leduc-Galindo, Per Qvist, Andrea E Tóth, Tue Fryland, Morten S Nielsen, Anders D Børglum, Jane H Christensen
The blood-brain barrier consists of a tightly sealed monolayer of endothelial cells being vital in maintaining a stable intracerebral microenvironment. The barrier is receptive to leakage upon exposure to environmental factors, like hypoxia, and its disruption has been suggested as a constituent in the pathophysiology of both neurological and psychiatric disorders. The schizophrenia associated ZEB1 gene encodes a transcription factor susceptible to transcriptional control by a hypoxia induced factor, HIF1A, known to be implicated in blood-brain barrier dysfunction...
August 23, 2018: Microvascular Research
Marie-Pierre Cyr, Alexandra Pinard, Olivia Dubois, Mélanie Morin
BACKGROUND: Microvascular assessment has become increasingly used in gynecology to better understand the pathophysiology of various vulvar conditions and to study sexual function. Alteration in blood perfusion of the vulvar area has been observed using laser technologies in women with provoked vestibulodynia (PVD), the leading cause of vulvar pain. However, no studies have thus far investigated the reliability of and agreement between lasers for evaluating vulvar blood perfusion. OBJECTIVES: The main objective was to investigate the between-session reliability of the laser Doppler perfusion imaging (LDPI) and laser speckle contrast imaging (LSCI) for assessing blood perfusion of the vulvar vestibule in women with PVD...
August 16, 2018: Microvascular Research
Wellington Santana da Silva Júnior, Maria das Graças Coelho de Souza, José Firmino Nogueira Neto, Eliete Bouskela, Luiz Guilherme Kraemer-Aguiar
OBJECTIVE: In patients with diabetes, dipeptidyl peptidase 4 (DPP4) inhibition is associated with attenuation of inflammation and endothelial dysfunction. Here, we investigated the associations between constitutive DPP4 activity, inflammatory biomarkers, and microvascular reactivity in subjects with excess body weight without diabetes. METHODS: Forty subjects of BMI ≥ 25.0 kg/m2 and without diabetes were cross-sectionally evaluated. We assessed microvascular blood flow and vasomotion by laser Doppler flowmetry, and measured at baseline, 30, and 60 min after a standardized meal: DPP4 activity, glucose, insulin, hs-CRP, TNF-α, IL-6, PAI-1, ICAM-1, and VCAM-1...
July 30, 2018: Microvascular Research
Kumiko Kaifu, Seiji Ueda, Nobutaka Nakamura, Takanori Matsui, Nana Yamada-Obara, Ryotaro Ando, Yusuke Kaida, Masami Nakata, Maki Matsukuma-Toyonaga, Yuichiro Higashimoto, Kei Fukami, Yusuke Suzuki, Seiya Okuda, Sho-Ichi Yamagishi
We have previously shown that albuminuria and renal levels of advanced glycation end products (AGEs), receptor for AGEs (RAGE), and oxidative stress are suppressed in dipeptidyl peptidase-4 (DPP-4)-deficient diabetic rats, thus suggesting the crosstalk between AGE-RAGE axis and DPP-4 in experimental diabetic nephropathy. Therefore, we examined here the role of DPP-4 in AGE-evoked inflammatory reactions in human proximal tubular cells. Proteins were extracted from proximal tubular cells, and conditioned medium was collected, both of which were subjected to western blot analysis using anti-DPP-4 antibody...
July 26, 2018: Microvascular Research
Henner Hanssen, Alice Minghetti, Oliver Faude, Arno Schmidt-Trucksäss, Lukas Zahner, Johannes Beck, Lars Donath
BACKGROUND: Psychiatric disorders are associated with a high prevalence of cardiovascular disease. Regular exercise is known to reduce depressive symptoms and improve vascular function, in turn lowering cardiovascular risk. We aimed to investigate the effects of different exercise modalities on retinal vessel diameters as a microvascular biomarker and depression severity index in patients suffering from unipolar depression. METHODS: 23 patients (female: 19, male: 4, age: 36...
July 24, 2018: Microvascular Research
A Gandecka, A Araszkiewicz, S Piłaciński, B Wierusz-Wysocka, D Zozulińska-Ziółkiewicz
AIM: The aim of this study was to assess the relationship between sudomotor function and microvascular perfusion in patients with type 1 diabetes (DM1). METHODS: We evaluated 415 patients (206 women), with DM1, median age of 41 (IQR: 33-53) years, disease duration of 25 (IQR: 20-32) years. We assessed metabolic control of diabetes and the presence of peripheral and cardiac autonomic neuropathy. Sudomotor function was assessed using Sudoscan device by electrochemical skin conductance (ESC)...
July 22, 2018: Microvascular Research
Alexa Caldwell, Jan Niklas Morick, Anne-Marie Jentsch, Annette Wegner, Dragan Pavlovic, Nadia Al-Banna, Christian Lehmann
BACKGROUND: Sepsis involves dysfunctional glucose metabolism. Among patients with sepsis, hyperglycemia is frequent and insulin administration has been evaluated for glycemic control to improve patient outcomes. Only few studies have examined the hyperglycemic microcirculation and the impact of insulin on the microvasculature in sepsis. OBJECTIVE: To study the functional capillary density (FCD) and leukocyte activation within the intestinal microcirculation in endotoxin-induced experimental sepsis...
September 2018: Microvascular Research
Xiaoyu Wang, Xuejie Han, Minghui Li, Yu Han, Yun Zhang, Shiqi Zhao, Yue Li
Ticagrelor has been reported to decrease cardiovascular mortality compared with clopidogrel. This benefit cannot be fully explained by the more efficient platelet inhibition. Many studies demonstrated that ticagrelor improved endothelial function, leaving the mechanism elusive though. The present study aims to investigate whether ticagrelor protects against endothelial dysfunction induced by angiotensinII (AngII) through alleviating endoplasmic reticulum (ER) stress. Male Sprague Dawley rats were infused with AngII or vehicle and administrated with ticagrelor or vehicle for 14 days...
September 2018: Microvascular Research
Hooman Salavati, M Soltani, Saeid Amanpour
The mechanisms involved in tumor growth mainly occur at the microenvironment, where the interactions between the intracellular, intercellular and extracellular scales mediate the dynamics of tumor. In this work, we present a multi-scale model of solid tumor dynamics to simulate the avascular and vascular growth as well as tumor-induced angiogenesis. The extracellular and intercellular scales are modeled using partial differential equations and cellular Potts model, respectively. Also, few biochemical and biophysical rules control the dynamics of intracellular level...
September 2018: Microvascular Research
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