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FEBS Letters

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https://www.readbyqxmd.com/read/28653419/conformational-switch-of-harmonin-a-submembrane-scaffold-protein-of-the-hair-cell-mechanoelectrical-transduction-machinery
#1
LETTER
Amel Bahloul, Elise Pepermans, Bertrand Raynal, Nicolas Wolff, Florence Cordier, Patrick England, Sylvie Nouaille, Bruno Baron, Aziz El-Amraoui, Jean-Pierre Hardelin, Dominique Durand, Christine Petit
Mutations in the gene encoding harmonin, a multi-PDZ domain-containing submembrane protein, cause Usher syndrome type 1 (congenital deafness and balance disorder, as well as early-onset sight loss). The structure of the protein and biological activities of its three different classes of splice isoforms (a, b, and c) remain poorly understood. Combining biochemical and biophysical analyses, we show that harmonin-a1 can switch between open and closed conformations through intramolecular binding of its C-terminal PDZ-binding motif to its N-terminal supramodule NTD-PDZ1 and a flexible PDZ2-PDZ3 linker...
June 27, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28649760/immunometabolism-of-human-autoimmune-diseases-from-metabolites-to-extracellular-vesicles
#2
REVIEW
Paola de Candia, Veronica De Rosa, Vincenzo Gigantino, Gerardo Botti, Antonio Ceriello, Giuseppe Matarese
Immunometabolism focuses on the mechanisms regulating the impact of metabolism on lymphocyte activity and autoimmunity outbreak. The adipose tissue is long known to release adipokines, either pro- or anti-inflammatory factors bridging nutrition and immune function. More recently, adipocytes were discovered to also release extracellular vesicles (EVs) containing a plethora of biological molecules, including metabolites and microRNAs, which can regulate cell function/metabolism in distant tissues, suggesting that immune regulatory function by the adipose tissue may be far more complex than originally thought...
June 26, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28649756/lacto-ghrestatin-a-novel-bovine-milk-derived-peptide-suppresses-ghrelin-secretion
#3
LETTER
Hayato Aoki, Junya Nakato, Takafumi Mizushige, Hiroshi Iwakura, Masaru Sato, Hideyuki Suzuki, Ryuhei Kanamoto, Kousaku Ohinata
Ghrelin, an endogenous peptide isolated from the stomach, is known to stimulate food intake after peripheral administration. We found that the enzymatic digest of β-lactoglobulin decreases ghrelin secretion from the ghrelin-producing cell line MGN3-1. The peptides present in the digest were comprehensively analyzed using the nanoLC-OrbitrapMS. Among them, we identified that the nonapeptide LIVTQTMKG, corresponding to β-lactoglobulin(1-9), suppresses ghrelin secretion from MGN3-1 cells. We named LIVTQTMKG 'lacto-ghrestatin'...
June 26, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28649728/death-by-translation-ribosome-assisted-degradation-of-mrna-by-endonuclease-toxins
#4
Joel G Belasco
No abstract text is available yet for this article.
June 26, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28649717/dissecting-the-variation-in-transcriptional-circuits-between-na%C3%A3-ve-and-primed-pluripotent-states
#5
LETTER
Aritra Deb, Arijita Sarkar, Zhumur Ghosh
Naive and primed pluripotent states are very similar to each other, but subtle differences exist in their maintenance and differentiation programs. Transcription factors (TFs) play a key role towards maintaining pluripotency and cellular reprogramming. However, TF expression dynamics and regulatory mechanisms in naive and primed pluripotent states are poorly understood. Here, we performed a comprehensive transcriptional analysis of both states, which revealed a gene expression pattern in mESCs (naïve state) that appear to be distinct from mEpiSCs (primed state)...
June 26, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28649707/coordination-of-the-third-step-of-protein-splicing-in-two-cyanobacterial-inteins
#6
LETTER
Christian K Ramsoomair, Anthony E Yakely, Laura M Urbanski, Kevin Karanja, Zachary T Giaccone, Nicolle M Siegart, Chunyu Wang, Alvin V Gomez, Julie N Reitter, Kenneth V Mills
The third step of protein splicing is cyclization of Asn coupled to peptide bond cleavage. In two related cyanobacterial inteins, this step is facilitated by Asn or Gln. For a Synechococcus sp. PCC7002 intein, the isolated third step of protein splicing is more efficient with its native Asn than with substitution to Gln. For a Trichodesmium erythraeum intein, its native Gln facilitates the third step as efficiently as with Asn. Despite these differences, the yield of splicing is not affected, suggesting that the third step is influenced by mechanism-linked conformational changes...
June 26, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28644554/n-5-n-10-methylenetetrahydromethanopterin-reductase-from-methanocaldococcus-jannaschii-also-serves-as-a-methylglyoxal-reductase
#7
LETTER
Danielle V Miller, Michelle Ruhlin, W Keith Ray, Huimin Xu, Robert H White
In Methanocaldococcus jannaschii, methylglyoxal is required for aromatic amino acid biosynthesis. Previously, the reduction of methylglyoxal to lactaldehyde in M. jannaschii cell extracts using either NADPH or F420 H2 was demonstrated; however, the enzyme responsible was not identified. Using NADPH as the reductant, the unknown enzyme was purified from cell extracts of M. jannaschii and determined to be the F420 -dependent N(5) , N(10) -methylenetetrahydromethanopterin reductase (Mer). Here, we report that the recombinantly overexpressed Mer is able to use NADPH and methylglyoxal (KM of 1...
June 23, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28643334/gastric-cancer-derived-exosomes-promote-peritoneal-metastasis-by-destroying-the-mesothelial-barrier
#8
LETTER
Guang Deng, Jinglei Qu, Ye Zhang, Xiaofang Che, Yu Cheng, Yibo Fan, Simeng Zhang, Di Na, Xiujuan Qu, Yunpeng Liu
An intact mesothelium serves as a protective barrier to inhibit peritoneal carcinomatosis. Cancer-derived exosomes can mediate directional tumor metastasis; however, little is known about whether gastric cancer-derived exosomes will destroy the mesothelial barrier and promote peritoneal dissemination. Here, we demonstrate that gastric cancer-derived exosomes facilitate peritoneal metastasis by causing mesothelial barrier disruption and peritoneal fibrosis. Injury of peritoneal mesothelial cells elicited by gastric cancer-derived exosomes is through concurrent apoptosis and mesothelial-to-mesenchymal transition (MMT)...
June 22, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28640976/subconductance-states-of-mitochondrial-chloride-channels-implication-for-functionally-coupled-tetramers
#9
LETTER
Tomasek Milan, Anton Misak, Marian Grman, Zuzana Tomaskova
Recently, it has been discovered that isoforms of intracellular chloride channels (CLIC) are present in cardiac mitochondria. By reconstituting rat cardiac mitochondrial chloride channels into bilayer lipid membranes, we detected three equally separated subconductance states with conductance increment of 45 pS and <2% occupancy. The observed rare events of channel decomposition into substates, accompanied by disrupted gating, provide an insight into channel quaternary structure. Our findings suggest that the observed channels work as four functionally- coupled subunits with synchronized gating...
June 22, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28640956/mir-188-promotes-senescence-of-lineage-negative-bone-marrow-cells-by-targeting-map3k3-expression
#10
LETTER
Yue Zheng, Hua Liu, Ye Kong
Lin-BMCs have reparative potential for overcoming endothelial dysfunction and reducing cardiovascular risk. Here, we found that miR-188 is upregulated and MAP3K3 is downregulated in aged lin-BMCs, whereas their expression is reversed in young lin-BMCs. We identified and confirmed MAP3K3 as a direct target of miR-188. MiR-188 overexpression or MAP3K3 silencing in young lin-BMCs increases p16 and p21 expression, enhances cell senescence, and decreases the ability for cell proliferation, migration, and tube formation...
June 22, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28640525/early-stages-in-the-biogenesis-of-eukaryotic-%C3%AE-barrel-proteins
#11
REVIEW
Tobias Jores, Doron Rapaport
The endosymbiotic organelles mitochondria and chloroplasts harbour, similarly to their prokaryotic progenitors, β-barrel proteins in their outer membrane. These proteins are encoded on nuclear DNA, translated on cytosolic ribosomes, and imported into their target organelles by a dedicated machinery. Recent studies have provided insights into the import into the organelles and the membrane insertion of these proteins. Although the cytosolic stages of their biogenesis are less well defined, it is speculated that upon their synthesis, chaperones prevent β-barrel proteins from aggregation and keep them in an import-competent conformation...
June 22, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28640495/control-of-mus81-nuclease-during-the-cell-cycle
#12
REVIEW
Boris Pfander, Joao Matos
DNA replication and homologous recombination rely on the formation of branched DNA structures that physically link chromosomes. Such DNA-based connections, which arise during S phase, are typically disengaged prior to entry into mitosis, in order to ensure proper chromosome segregation. Exceptions can, however, occur: replication stress, or elevated levels of DNA damage, may cause cells to enter mitosis with unfinished replication as well as carrying recombination intermediates, such as Holliday junctions. Hence, cells are equipped with pathways that recognize and process branched DNA structures, and evolved mechanisms to enhance their function when on the verge of undergoing cell division...
June 22, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28640390/transiently-disordered-tails-accelerate-folding-of-globular-proteins
#13
LETTER
Saurav Mallik, Tanaya Ray, Sudip Kundu
Numerous biological proteins exhibit intrinsic disorder at their termini, which are associated with multifarious functional roles. Here, we show the surprising result that an increased percentage of terminal short transiently disordered regions with enhanced flexibility (TstDREF) is associated with accelerated folding rates of globular proteins. Evolutionary conservation of predicted disorder at TstDREFs and drastic alteration of folding rates upon point-mutations suggest critical regulatory role(s) of TstDREFs in shaping the folding kinetics...
June 22, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28640365/the-c-elegans-wrn-helicase-promotes-double-strand-dna-break-repair-by-mediating-end-resection-and-checkpoint-activation
#14
LETTER
Jin-Sun Ryu, Hyeon-Sook Koo
The protein associated with Werner syndrome, WRN, is involved in DNA repair, checkpoint activation, and telomere maintenance. To better understand the involvement of WRN in double-strand DNA break (DSB) repair, we analyzed the combinatorial role of WRN-1, the C. elegans WRN helicase, in conjunction with EXO-1 and DNA-2 nucleases. We found that WRN-1 cooperates with DNA-2 to resect DSB ends in a pathway acting in parallel to EXO-1. The wrn-1 mutants show an aberrant accumulation of RPA and RAD-51, and the same pattern of accumulation is also observed in checkpoint-defective strains...
June 22, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28640363/small-molecule-stabilization-of-the-p53-14-3-3-protein-protein-interaction
#15
LETTER
Richard G Doveston, Ave Kuusk, Sebastian Andrei, Seppe Leysen, Qing Cao, Paola Castaldi, Adam Hendricks, Hongming Chen, Helen Boyd, Christian Ottmann
14-3-3 proteins are positive regulators of the tumor suppressor p53, the mutation of which is implicated in many human cancers. Current strategies for targeting of p53 involve restoration of wild-type function or inhibition of the interaction with MDM2, its key negative regulator. Despite the efficacy of these strategies, the alternate approach of stabilizing the interaction of p53 with positive regulators and, thus, enhancing tumor suppressor activity, has not been explored. Here, we report the first example of small-molecule stabilization of the 14-3-3 - p53 protein-protein interaction and demonstrate the potential of this approach as a therapeutic modality...
June 22, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28627101/depletion-of-cdc-25-3-a-c-elegans-ortholog-of-cdc25-increases-physiological-germline-apoptosis
#16
LETTER
Minhee Sung, Ichiro Kawasaki, Yhong-Hee Shim
In Caenorhabditis elegans hermaphrodites, physiological germline apoptosis is higher in cdc-25.3 mutants than in wild type. The elevated germline apoptosis in cdc-25.3 mutants seems to be induced by accumulation of double-stranded DNA breaks (DSBs). Both DNA-damage and synapsis checkpoint genes are required to increase the germline apoptosis. Notably, the number of germ cells that lose P-granule components, PGL-1 and PGL-3, increase in cdc-25.3 mutants, and the increase in germline apoptosis requires the activity of SIR-2...
June 19, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28626879/snipper-an-eri1-homologue-affects-histone-mrna-abundance-and-is-crucial-for-normal-drosophila-melanogaster-development
#17
LETTER
Alexiadis Anastasios, Delidakis Christos, Kalantidis Kriton
The conserved 3'-5' RNA exonuclease ERI1 is implicated in RNA interference inhibition, 5.8S rRNA maturation, and histone mRNA maturation and turnover. The single ERI1 homologue in Drosophila melanogaster Snipper (Snp) is a 3'-5' exonuclease, but its in vivo function remains elusive. Here, we report Snp requirement for normal Drosophila development, since its perturbation leads to larval arrest and tissue-specific downregulation results in abnormal tissue development. Additionally, Snp directly interacts with histone mRNA, and its depletion results in drastic reduction in histone transcript levels...
June 19, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28626869/identification-of-mirnas-involved-in-drg-neurite-outgrowth-and-their-putative-targets
#18
LETTER
Dario Motti, Jessica K Lerch, Matt C Danzi, Jared H Gans, Frank Kuo, Tatiana I Slepak, John L Bixby, Vance P Lemmon
Peripheral neurons regenerate their axons after injury. Transcriptional regulation by microRNAs (miRNAs) is one possible mechanism controlling regeneration. We profiled miRNA expression in mouse dorsal root ganglion (DRG) neurons after a sciatic nerve crush, and identified 49 differentially expressed miRNAs. We evaluated the functional role of each miRNA using a phenotypic analysis approach. To predict the targets of the miRNAs we employed RNA-Sequencing and examined transcription at the isoform level. We identify thousands of differentially expressed isoforms and bioinformatically associate the miRNAs that modulate neurite growth with their putative target isoforms to outline a network of regulatory events underlying peripheral nerve regeneration...
June 19, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28627120/new-insights-into-human-primordial-germ-cells-and-early-embryonic-development-from-single-cell-analysis
#19
REVIEW
Jörg Otte, Wasco Wruck, James Adjaye
Human preimplantation developmental studies are difficult to accomplish due to associated ethical and moral issues. Preimplantation cells are rare and exist only in transient cell states. From a single cell, it is very challenging to analyse the origination of the heterogeneity and complexity inherent to the human body. However, recent advances in single-cell technology and data analysis have provided new insights into the process of early human development and germ cell specification. In this Review, we examine the latest single-cell datasets of human preimplantation embryos and germ cell development, compare them to bulk cell analyses, and interpret their biological implications...
June 18, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28617934/the-anaerobic-biosynthesis-of-plasmalogens
#20
REVIEW
Howard Goldfine
The biosynthesis of plasmalogens in anaerobic bacteria differs fundamentally from that in animal cells. Firstly, the formation of the alk-1'-enyl ether bond in animal cells is oxygen-dependent. Secondly, the first step in plasmalogen formation in animal cells is an acylation of dihydroxyacetonephosphate (DHAP), which has been ruled out as a precursor in anaerobes. In bacteria the alk-1'-enyl ether bond is formed after the fully formed acyl glycerolipids are synthesized. Evidence will be presented for the conversion of the sn-1 acyl-linked chain to an O-alk-1'-enyl ether by an as yet unknown mechanism...
June 15, 2017: FEBS Letters
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