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FEBS Letters

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https://www.readbyqxmd.com/read/28304078/moving-messages-in-the-developing-brain-emerging-roles-for-mrna-transport-and-local-translation-in-neural-stem-cells
#1
REVIEW
Louis-Jan Pilaz, Debra L Silver
The mammalian cerebral cortex is a complex brain structure integral to our higher cognition. During embryonic cortical development, radial glial progenitors (RGCs) produce neurons and serve as physical structures for migrating neurons. Recent discoveries highlight new roles for RNA localization and local translation in RGCs, both at the cell body and at distal structures called basal endfeet. By implementing technologies from the field of RNA research to brain development, investigators can manipulate RNA binding proteins as well as visualize single molecule RNAs, live movement of mRNAs and their binding proteins, and translation...
March 17, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28303570/aggregation-of-mod5-is-affected-by-trna-binding-with-implications-for-trna-gene-mediated-silencing
#2
LETTER
D F Read, T J Waller, E Tse, D R Southworth, D R Engelke, P J Smaldino
Mod5 is a multifunctional protein that modifies a subset of tRNAs in the cytoplasm and is also required for an RNA-mediated form of transcriptional silencing. Previous in vivo studies have shown that the nuclear silencing function of Mod5 does not require that the causative tRNA gene encode a Mod5 substrate, though Mod5 is still required. However, previous data have not directly tested whether Mod5 can directly bind substrate and nonsubstrate RNAs. We herein demonstrate that Mod5 directly binds to both substrate and nonsubstrate RNAs, including a highly structured, non-tRNA sequence (5S-rRNA), consistent with previous in vivo data...
March 17, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28294307/smurf1-controls-s-phase-progression-and-tumorigenesis-through-wee1-degradation
#3
LETTER
Rongfei Wei, Jing Guo, Mengyuan Li, Xingjiu Yang, Ruimin Zhu, Hao Huang, Kejuan Li, Lingqiang Zhang, Ran Gao
Smad ubiquitination regulatory factor 1 (Smurf1) is a HECT-type E3 ubiquitin ligase that regulates several important signaling pathways, including the bone morphogenetic protein pathway and the transforming growth factor-beta (TGF-β) signaling pathway. However, the function of Smurf1 in cell cycle progression remains unclear. Here, we demonstrate that silencing of Smurf1 results in S phase arrest, confirming that Smurf1 is required for S phase progression. Furthermore, we demonstrate that Smurf1-mediated S phase progression is largely dependent on the ubiquitination-dependent degradation of Wee1...
March 14, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28295271/nlrp6-facilitates-the-interaction-between-tab2-3-and-trim38-in-rheumatoid-arthritis-fibroblast-like-synoviocytes
#4
LETTER
Yang Lin, Zhengqiang Luo
In the present study, we investigated the role of NOD-like receptor family pyrin domain containing 6 (NLRP6) in rheumatoid arthritis (RA) and explored the underlying mechanism. We found that both mRNA and protein levels of NLRP6 are attenuated in synovial tissues and fibroblast-like synoviocytes (FLS) of RA patients compared to patients with osteoarthritis (OA). We also observed that pro-inflammatory cytokine production is decreased and NF-κB activation is inhibited in NLRP6-overexpressing RA-FLS. Furthermore, we found that NLRP6 overexpression promotes TAB2/3 lysosome-dependent degradation, and we provide evidence to illustrate that NLRP6 plays the role of providing the docking site to facilitate the interaction between TAB2/3 and TRIM38 in RA-FLS...
March 10, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28295264/the-adaptors-grb10-and-grb14-are-calmodulin-binding-proteins
#5
LETTER
Irene García-Palmero, Noemí Pompas-Veganzones, Eduardo Villalobo, Sophie Gioria, Jacques Haiech, Antonio Villalobo
We identified the Grb7 family members, Grb10 and Grb14, as Ca(2+) -dependent CaM-binding proteins using Ca(2+) -dependent CaM-affinity chromatography. The potential CaM-binding sites were identified and experimentally tested using fluorescent-labelled peptides corresponding to these sites. The apparent affinity constant of these peptides for CaM, and the minimum number of calcium ions bound to CaM that are required for effective binding to these peptides were also determined. We prepared deletion mutants of the three adaptor proteins lacking the identified sites and determined that they lost or strongly diminished their CaM-binding capacity following the sequence Grb7 > Grb14 > Grb10...
March 10, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28295262/spatially-and-temporally-regulating-translation-via-mrna-binding-proteins-in-cellular-and-neuronal-function
#6
REVIEW
P G Donlin-Asp, W Rossoll, G J Bassell
Coordinated regulation of mRNA localization and local translation are essential steps in cellular asymmetry and function. It is increasingly evident that mRNA binding proteins play critical functions in controlling the fate of mRNA, including when and where translation occurs. In this review, we discuss the robust and complex roles that mRNA binding proteins play in the regulation of local translation that impact cellular function in vertebrates. First, we discuss the role of local translation in cellular polarity and possible links to vertebrate development and patterning...
March 10, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28281299/investigating-the-effect-of-available-redox-protein-ratios-for-the-conversion-of-a-steroid-by-a-myxobacterial-cyp260a1
#7
LETTER
Yogan Khatri, Alexander Schifrin, Rita Bernhardt
Since cytochromes P450 are external monooxygenases, available surrogate redox partners have been used to reconstitute the P450 activity. However, the effect of various ratios of P450s and the redox proteins have not been extensively studied so far, although different combinations of the redox partners have shown variations in substrate conversion. To address this issue, CYP260A1 was reconstituted with various ratios of adrenodoxin and adrenodoxin reductase to convert 11-deoxycorticosterone, and the products were characterized by NMR...
March 9, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28281280/a-key-role-of-nuclear-factor-y-in-the-refeeding-response-of-fatty-acid-synthase-in-adipocytes
#8
LETTER
Makiko Nishi-Tatsumi, Naoya Yahagi, Yoshinori Takeuchi, Naoki Toya, Ayako Takarada, Yuki Murayama, Yuichi Aita, Yoshikazu Sawada, Xiaoying Piao, Yukari Oya, Akito Shikama, Yukari Masuda, Midori Kubota, Yoshihiko Izumida, Takashi Matsuzaka, Yoshimi Nakagawa, Motohiro Sekiya, Yoko Iizuka, Yasushi Kawakami, Takashi Kadowaki, Nobuhiro Yamada, Hitoshi Shimano
Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad-luc analytical system, we identified the inverted CCAAT element (ICE) around -100 nucleotides in the Fasn promoter as a critical cis-element for the refeeding response in adipocytes...
March 9, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28267209/identification-of-primary-and-secondary-uba-footprints-on-the-surface-of-ubiquitin-in-cell-mimicking-crowded-solution
#9
LETTER
Francesca Munari, Andrea Bortot, Serena Zanzoni, Mariapina D'Onofrio, David Fushman, Michael Assfalg
Despite significant advancements in our understanding of ubiquitin-mediated signaling, the influence of the intracellular environment on formation of transient ubiquitin-partner complexes remains poorly explored. In our work, we introduce macromolecular crowding as a first level of complexity towards the imitation of a cellular environment in the study of such interactions. Using NMR spectroscopy, we find that the stereospecific complex of ubiquitin and ubiquitin-associated domain (UBA) is minimally perturbed by the crowding agent Ficoll...
March 7, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28267204/c-terminal-truncation-of-gsk-3%C3%AE-enhances-its-dephosphorylation-by-pp2a
#10
LETTER
Nana Jin, Yue Wu, Wen Xu, Cheng-Xin Gong, Khalid Iqbal, Fei Liu
Glycogen synthase kinase-3β (GSK-3β) is the major tau kinase. Its phosphorylation at Ser9 suppresses the activity. In Alzheimer's disease (AD) brain, GSK-3β is truncated at the C-terminus by over-activated calpain I, leading to an increase in its activity. However, the effect of truncation on its phosphorylation is unknown. We found here that in AD brain and in cultured cells, C-terminally truncated GSK-3β is less phosphorylated at Ser9 than the full-length enzyme. The truncation promotes GSK-3β nuclear translocation and enhances its interaction with protein phosphatase 2A (PP2A), leading to dephosphorylation...
March 7, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28267202/the-role-of-histidines-in-amyloid-%C3%AE-fibril-assembly
#11
LETTER
Kristoffer Brännström, Tohidul Islam, Linda Sandblad, Anders Olofsson
Low pH has a strong stabilising effect on the fibrillar assembly of amyloid β, which is associated with Alzheimer's disease. The stabilising effect is already pronounced at pH 6.0, suggesting that protonation of histidines might mediate this effect. Through the systematic substitution of the three native histidines in Aβ for alanines, we have evaluated their role in fibril stability. Using surface plasmon resonance, we show that at neutral pH the fibrillar forms of all His-Ala variants are destabilised by a factor of 4-12 compared to wild-type Aβ...
March 7, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28263378/akin10-a-representative-arabidopsis-snf1-related-protein-kinase-1-snrk1-phosphorylates-and-downregulates-plant-hmg-coa-reductase
#12
LETTER
Jekson Robertlee, Keiko Kobayashi, Masashi Suzuki, Toshiya Muranaka
HMG-CoA reductase (HMGR) is a key enzyme in the mevalonate pathway for sterols and cytosolic isoprenoid production. Although HMGR kinases from spinach, barley, and cauliflower tissues have been strongly suggested as members of SNF1-related protein kinases 1 (SnRK1), the phosphorylation and inactivation of HMGR by plant SnRK1s has not been demonstrated. In this study, we elucidated that AKIN10, an Arabidopsis SnRK1, acts as an HMGR kinase. The recombinant AKIN10 phosphorylates and inactivates AtHMGR1S using recombinant GRIK1 as the AKIN10 activator...
March 6, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28247503/characterization-of-an-extradiol-dioxygenase-involved-in-the-catabolism-of-lignin-derived-biphenyl
#13
LETTER
Eugene Kuatsjah, Hong-Ming Chen, Stephen G Withers, Lindsay D Eltis
In the catabolism of lignin-derived biphenyl by Sphingobium sp. SYK-6, LigZ catalyzes the cleavage of 2,2',3-trihydroxy-3'-methoxy-5,5'-dicarboxybiphenyl (OH-DDVA) to a meta-cleavage product (MCP) identified here as 4,11-dicarboxy-8-hydroxy-9-methoxy-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (DCHM-HOPDA). DCHM-HOPDA is transformed non-enzymatically, likely to a lactone (k = 0.13±0.01 min(-1) , pH 7.5). This is hydrolyzed to the dienolate at alkaline pH (apparent pKa ~11.3). Only the dienolate is a substrate for LigY, the putative MCP hydrolase...
March 1, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28245055/c-terminal-dimerization-of-apo-cyclic-amp-receptor-protein-validated-in-solution
#14
Dae-Won Sim, Jae-Wan Choi, Ji-Hun Kim, Kyoung-Seok Ryu, Myeongkyu Kim, Hee-Wan Yu, Ku-Sung Jo, Eun-Hee Kim, Min-Duk Seo, Young-Ho Jeon, Bong-Jin Lee, Young Pil Kim, Hyung-Sik Won
Although cyclic AMP receptor protein (CRP) has long served as a typical example of effector-mediated protein allostery, mechanistic details into its regulation have been controversial due to discrepancy between the known crystal structure and NMR structure of apo-CRP. Here, we report that the recombinant protein corresponding to its C-terminal DNA-binding domain (CDD) forms a dimer. This result, together with structural information obtained in the present NMR study, is consistent with the previous crystal structure and validates its relevance also in solution...
February 28, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28245054/fission-yeast-apc-c-activators-slp1-and-fzr1-sequentially-trigger-two-consecutive-nuclear-divisions-during-meiosis
#15
LETTER
Yuji Chikashige, Miho Yamane, Kasumi Okamasa, Hiroko Osakada, Chihiro Tsutsumi, Yuki Nagahama, Noriko Fukuta, Tokuko Haraguchi, Yasushi Hiraoka
In meiosis, two rounds of nuclear division occur consecutively without DNA replication between the divisions. We isolated a fission yeast mutant in which the nucleus divides only once to generate two spores, as opposed to four, in meiosis. In this mutant, we found that the initiation codon of the slp1(+) gene is converted to ATA, producing a reduced amount of Slp1. As a member of the Fizzy family of APC/C activators, Slp1 is essential for vegetative growth; however, the mutant allele shows a phenotype only in meiosis...
February 28, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28236657/gfh-factors-and-nusa-cooperate-to-stimulate-transcriptional-pausing-and-termination
#16
LETTER
Aleksei Agapov, Anna Olina, Daria Esyunina, Andrey Kulbachinskiy
Lineage-specific Gfh factors from the radioresistant bacterium Deinococcus radiodurans, which bind within the secondary channel of RNA polymerase, stimulate transcriptional pausing at a wide range of pause signals (elemental, hairpin-dependent, post-translocated, backtracking-dependent, consensus pauses) and increase intrinsic termination. Universal bacterial factor NusA, which binds near the RNA exit channel, enhances the effects of Gfh factors on termination and hairpin-dependent pausing but do not act on other pause sites...
February 25, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28236654/sortilin-1-knockout-alters-basal-adipose-glucose-metabolism-but-not-diet-induced-obesity-in-mice
#17
Jibiao Li, David J Matye, Yifeng Wang, Tiangang Li
Sortilin 1 (Sort1) is a trafficking receptor that has been implicated in the regulation of plasma cholesterol in humans and mice. Here, we use metabolomics and hyperinsulinemic-euglycemic clamp approaches to obtain further understanding of the in vivo effects of Sort1 deletion on diet-induced obesity as well as on adipose lipid and glucose metabolism. Results show that Sort1 knockout does not affect Western diet-induced obesity or adipose fatty acid and ceramide concentrations. Under the basal fasting state, chow-fed Sort1 knockout mice have decreased adipose glycolytic metabolites, but Sort1 deletion does not affect insulin-stimulated tissue glucose uptake during the insulin clamp...
February 25, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28236646/an-invertebrate-sting-from-shrimp-activates-an-innate-immune-defense-against-bacterial-infection
#18
Haoyang Li, Sheng Wang, Kai Lǚ, Bin Yin, Bang Xiao, Sedong Li, Jianguo He, Chaozheng Li
It has been proposed that invertebrate stimulators of interferon genes (STINGs) do not take part in the innate immune response to infection. Herein, we identified a new STING homolog from pacific white shrimp Litopenaeus vannamei (LvSTING). Some amino acids crucial for recognizing cyclic dinucleotides in mammals are highly conserved in LvSTING. Moreover, LvSTING expression can be robustly induced by challenge with the Gram-negative bacteria Vibrio parahaemolyticus. Silencing of LvSTING contributes to decreased expression of the antimicrobial peptide PEN4 and renders shrimp more susceptible to V...
February 25, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28236635/the-competing-endogenous-rna-network-of-cyp4z1-and-pseudogene-cyp4z2p-exerts-an-anti-apoptotic-function-in-breast-cancer
#19
LETTER
Cheng Li, Lufeng Zheng, Yu Xin, Zhoulin Tan, Yan Zhang, Xia Meng, Zhigang Wang, Tao Xi
The competing endogenous RNA network (ceRNET) is involved in tumorigenesis and has become a hot spot of research. Our previous studies have revealed that the ceRNET between CYP4Z1 and the pseudogene CYP4Z2P promotes angiogenesis and mediates tamoxifen resistance in breast cancer. Nevertheless, the effects of this ceRNET on cell apoptosis and related mechanisms remain unclear. In this study, we found that downregulation of CYP4Z1 or the CYP4Z2P 3'-UTR promotes cell apoptosis, mirroring the functions of human telomerase reverse transcriptase (hTERT)...
February 25, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28235243/the-microrna-mir-124-inhibits-vascular-smooth-muscle-cell-proliferation-by-targeting-s100-calcium-binding-protein-a4-s100a4
#20
LETTER
Nakwon Choe, Duk-Hwa Kwon, Sera Shin, Yong Sook Kim, Young-Kook Kim, Jaetaek Kim, Youngkeun Ahn, Gwang Hyeon Eom, Hyun Kook
S100 calcium-binding protein A4 (S100A4) induces proliferation and migration of vascular smooth muscle cells (VSMCs). We aimed to find the microRNA regulating S100A4 expression. S100A4 transcripts are abruptly increased in the acute phase of carotid arterial injury one day later (at day 1) but gradually decreases at days 7 and 14. Bioinformatics analysis reveal that miR-124 targets S100A4. VSMC survival is attenuated by miR-124 mimic but increased by miR-124 inhibitor. miR-124 decreases immediately after carotid arterial injury but dramatically increases at days 7 and 14...
February 24, 2017: FEBS Letters
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