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FEBS Letters

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https://www.readbyqxmd.com/read/28103624/biochemical-characterization-of-the-helicobacter-pylori-cag-t4ss-unique-component-cagu
#1
LETTER
Rajesh Kumari, Mohd Shariq, Navin Kumar, Gauranga Mukhopadhyay
Many strains of Helicobacter pylori encode a protein secreting type IV secretion system called the Cag-T4SS. Here, we characterize one of the Cag-T4SS specific components, CagU (HP0531). We report that CagU is a bacterial inner membrane-associated protein, partially processed at the C-terminus, and that it interacts with the VirB6 and VirB8 homologues CagW and CagV, respectively. The level of expression of CagU is partially affected in the absence of cagX, cagW and cagV. Deletion of cagU aborts surface localization of CagA and affects the expression levels of CagI and CagH, which are involved in the Cag-T4SS pilus formation...
January 19, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28101952/classical-biochemistry-reveals-the-complexity-of-the-mitochondrial-protein-import-system
#2
Dana Dayan, Abdussalam Azem
No abstract text is available yet for this article.
January 19, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28100013/cushing-s-syndrome-mutant-pka-l-205r-exhibits-altered-substrate-specificity
#3
LETTER
Joshua M Lubner, Kimberly L Dodge-Kafka, Cathrine R Carlson, George M Church, Michael F Chou, Daniel Schwartz
The PKA(L)(205R) hotspot mutation has been implicated in Cushing's Syndrome through hyperactive gain-of-function PKA signaling, however its influence on substrate specificity has not been investigated. Here, we employ the Proteomic Peptide Library (ProPeL) approach to create high-resolution models for PKA(WT) and PKA(L)(205R) substrate specificity. We reveal that the L205R mutation reduces canonical hydrophobic preference at the substrate P+1 position, and increases acidic preference in downstream positions...
January 18, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28094442/identifying-niche-mediated-regulatory-factors-of-stem-cell-phenotypic-state-a-systems-biology-approach
#4
Srikanth Ravichandran, Antonio Del Sol
Understanding how the cellular niche controls the stem cell phenotype is often hampered due to the complexity of variegated niche composition, its dynamics, and non-linear stem cell-niche interactions. Here, we propose a systems biology view that considers stem cell-niche interactions as a many-body problem amenable to simplification by the concept of mean field approximation. This enables approximation of the niche effect on stem cells as a constant field that induces sustained activation/inhibition of specific stem cell signaling pathways in all stem cells within heterogeneous populations exhibiting the same phenotype (niche determinants)...
January 17, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28094440/bidirectional-communication-between-sensory-neurons-and-osteoblasts-in-an-in-vitro-co-culture-system
#5
LETTER
Daisuke Kodama, Takao Hirai, Hisataka Kondo, Kazunori Hamamura, Akifumi Togari
Recent studies have revealed that the sensory nervous system is involved in bone metabolism. However, the mechanism of communication between neurons and osteoblasts is yet to be elucidated. In this study, we investigated the signaling pathways between sensory neurons of the dorsal root ganglion (DRG) and the osteoblast-like MC3T3-E1 cells by using an in vitro co-culture system. Our findings indicate that signal transduction from DRG-derived neurons to MC3T3-E1 cells is suppressed by antagonists of the AMPA receptor and the NK1 receptor...
January 17, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28094437/the-deubiquitinating-enzyme-usp50-regulates-inflammasome-activation-through-targeting-the-asc-adaptor-protein
#6
LETTER
Jae Young Lee, Dongyeob Seo, Jiyeon You, Sehee Chung, Jin Seok Park, Ji-Hyung Lee, Su Myung Jung, Youn Sook Lee, Seok Hee Park
NLRP3-mediated inflammasome activation promotes caspase-1-dependent production of IL-1β and requires the adaptor protein ASC. Compared to the priming and activation mechanisms of the inflammasome signaling pathway, post-translational ubiquitination/deubiquitination mechanisms controlling inflammasome activation have not been clearly addressed. We here demonstrate that the deubiquitinating enzyme USP50 binds to the ASC protein and subsequently regulates the inflammasome signaling pathway through deubiquitinating the lysine 63-linked polyubiquitination of ASC...
January 17, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28094435/new-interactions-between-tumor-suppressor-fhit-protein-and-a-non-hydrolyzable-analog-of-its-ap4-a-substrate
#7
LETTER
Agnieszka Krakowiak, Beata Kocoń-Rębowska, Rafał Dolot, Danuta Piotrzkowska
Fragile histidine triad protein (Fhit) is a protein which primarily hydrolyses dinucleoside polyphosphates. To investigate possible interactions between the protein and a substrate, we used a non-hydrolyzable phosphorothioate analog of Ap4 A, containing 5-bromo-2'-deoxyUridine instead of one adenosine residue. Photocrosslinking, followed by LC-MS experiments, determined a complex in which the probe was covalently linked to the NDSIYEELQK peptide (residues 110-119). The peptide was located within the "disordered" region, which is invisible in the known crystal structures of Fhit...
January 17, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28090648/disordering-promotes-epigenetic-order
#8
Gesche Tallen, Karl Riabowol
No abstract text is available yet for this article.
January 16, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28084023/crystal-structure-of-a-family-80-chitosanase-from-mitsuaria-chitosanitabida
#9
LETTER
Yutaka Yorinaga, Takashi Kumasaka, Masaki Yamamoto, Kensaku Hamada, Makoto Kawamukai
Chitosanases belong to glycoside hydrolase families 5, 7, 8, 46, 75 and 80 and hydrolyse glucosamine polymers produced by partial or full deacetylation of chitin. Herein, we determined the crystal structure of chitosanase from the β-proteobacterium Mitsuaria chitosanitabida (McChoA) at 1.75 Å resolution; the first structure of a family 80 chitosanase. McChoA is a 34 kDa extracellular protein of 301 amino acids that fold into two (upper and lower) globular domains with an active site cleft between them. Key substrate-binding features are conserved with family 24 lysozymes and family 46 chitosanases...
January 13, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28079283/rab8a-regulates-insulin-stimulated-glut4-translocation-in-c2c12-myoblasts
#10
LETTER
Hanbing Li, Liting Ou, Jiannan Fan, Mei Xiao, Cuifang Kuang, Xu Liu, Yonghong Sun, Yingke Xu
Rab proteins are important regulators of GLUT4 trafficking in muscle and adipose cells. It is still unclear which Rabs are involved in insulin-stimulated GLUT4 translocation in C2C12 myoblasts. In this study, we detect the colocalization of Rab8A with GLUT4 and the presence of Rab8A at vesicle exocytic sites by TIRFM imaging. Overexpression of dominant-negative Rab8A (T22N) diminishes insulin-stimulated GLUT4 translocation, while constitutively active Rab8A (Q67L) augments it. In addition, knockdown of Rab8A inhibits insulin-stimulated GLUT4 translocation, which is rescued by replenishment of RNAi-resistant Rab8A...
January 12, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28079255/moonlighting-at-replication-forks-a-new-life-for-homologous-recombination-proteins-brca1-brca2-and-rad51
#11
REVIEW
Arun Mouli Kolinjivadi, Vincenzo Sannino, Anna de Antoni, Hervé Técher, Giorgio Baldi, Vincenzo Costanzo
Coordination between DNA replication and DNA repair ensures maintenance of genome integrity, which is lost in cancer cells. Emerging evidence has linked Homologous Recombination (HR) proteins RAD51, BRCA1 and BRCA2 to the stability of nascent DNA. This function appears to be distinct from Double Strand Break (DSB) repair and is in part due to the prevention of MRE11-mediated degradation of nascent DNA at stalled forks. The role of RAD51 in fork protection resembles the activity described for its prokaryotic ortholog RecA, which prevents nuclease-mediated degradation of DNA and promotes replication fork restart in cells challenged by DNA damaging agents...
January 12, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28074470/evidence-that-oxidative-dephosphorylation-by-the-non-heme-fe-ii-%C3%AE-ketoglutarate-ump-oxygenase-occurs-by-stereospecific-hydroxylation
#12
LETTER
Anwesha Goswami, Xiaodong Liu, Wenlong Cai, Thomas P Wyche, Tim S Bugni, Maïa Meurillon, Suzanne Peyrottes, Christian Perigaud, Koichi Nonaka, Jürgen Rohr, Steven G Van Lanen
LipL and Cpr19 are non-heme, mononuclear Fe(II)-dependent, α-ketoglutarate (αKG):UMP oxygenases that catalyze the formation of CO2 , succinate, phosphate, and uridine-5'-aldehyde, the last of which is a biosynthetic precursor for several nucleoside antibiotics that inhibit bacterial translocase I (MraY). To better understand the chemistry underlying this unusual oxidative dephosphorylation and establish a mechanistic framework for LipL and Cpr19, we report herein the synthesis of two biochemical probes - [1',3',4',5',5'-(2) H]UMP and the phosphonate derivative of UMP - and their activity with both enzymes...
January 11, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28066891/new-clues-on-sidt2-being-a-cation-conducting-protein-in-lysosomal-membranes
#13
Norbert Klugbauer
No abstract text is available yet for this article.
January 8, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28054709/domain-analysis-of-ras-association-domain-family-member-6-rassf6-upon-interaction-with-mdm2
#14
LETTER
Aradhan Sarkar, Hiroaki Iwasa, Shakhawoat Hossain, Xiaoyin Xu, Takeru Sawada, Takanobu Shimizu, Junichi Maruyama, Kyoko Arimoto-Matsuzaki, Yutaka Hata
Tumor suppressor RASSF6 has Ras-association domain (RA) and Salvador/RASSF/Hippo domain (SARAH). RASSF6 antagonizes MDM2, stabilizes p53, and induces apoptosis and cell cycle arrest. We previously demonstrated the interaction between RASSF6 and MDM2, but did not determine how both proteins interact with each other. We have shown here that N-terminal, RA, and SARAH domains of RASSF6 interact with MDM2 at distinct regions. RA binds to the RING-finger region of MDM2 and stabilizes p53. SARAH binds RA and blocks the interaction between RA and MDM2...
January 5, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28032905/the-amino-acid-transporter-slc1a3-is-plasma-membrane-localised-in-adipocytes-and-its-activity-is-insensitive-to-insulin
#15
LETTER
James R Krycer, Daniel J Fazakerley, Rosemary J Cater, Kristen Thomas, Sheyda Naghiloo, James G Burchfield, Sean J Humphrey, Robert J Vandenberg, Renae M Ryan, David E James
The hormone insulin coordinates the catabolism of nutrients by protein phosphorylation. Phosphoproteomic analysis identified insulin-responsive phosphorylation of the Glu/Asp transporter SLC1A3/EAAT1 in adipocytes. The role of SLC1A3 in adipocytes is not well-understood. We show that SLC1A3 is localised to the plasma membrane and the major regulator of acidic amino acid uptake in adipocytes. However, its localisation and activity were unaffected by insulin or mutation of the insulin-regulated phosphosite. The latter was also observed using a heterologous expression system in Xenopus laevis oocytes...
December 29, 2016: FEBS Letters
https://www.readbyqxmd.com/read/28032897/adipocyte-specific-disruption-of-mouse-cnot3-causes-lipodystrophy
#16
LETTER
Xue Li, Masahiro Morita, Chisato Kikuguchi, Akinori Takahashi, Toru Suzuki, Tadashi Yamamoto
Lipodystrophy involves a loss of adipose tissue. In mice, disruption of adipose tissue Cnot3, a subunit of the CCR4-NOT deadenylase complex, causes adipose tissue anomalies. In Cnot3(ad-/-) mice, white adipose tissue (WAT) decreases concomitantly with enhanced inflammation, whereas brown adipose tissue (BAT) increases and contains larger lipid droplets. Cnot3(ad-/-) mice show hyperinsulinemia, hyperglycemia, insulin resistance, and glucose intolerance, and cannot maintain body temperature during cold exposure...
December 29, 2016: FEBS Letters
https://www.readbyqxmd.com/read/28032891/split-bioid-a-proximity-biotinylation-assay-for-dimerization-dependent-protein-interactions
#17
LETTER
Sofie De Munter, Janina Görnemann, Rita Derua, Bart Lesage, Junbin Qian, Ewald Heroes, Etienne Waelkens, Aleyde Van Eynde, Monique Beullens, Mathieu Bollen
The biotin-identification (BioID) protocol uses a mutant of the biotin ligase BirA (BirA*) fused to a protein-of-interest to biotinylate proximate proteins in intact cells. Here, we show that two inactive halves of BirA* separately fused to a catalytic and regulatory subunit of protein phosphatase PP1 reconstitute a functional BirA* enzyme upon heterodimerization of the phosphatase subunits. We also demonstrate that this BirA*-fragment complementation approach, termed split-BioID, can be used to screen for substrates and other protein interactors of PP1 holoenzymes...
December 29, 2016: FEBS Letters
https://www.readbyqxmd.com/read/28029689/identification-of-a-stable-complex-between-a-nife-hydrogenase-catalytic-subunit-and-its-maturation-protease
#18
LETTER
Marta Albareda, Grant Buchanan, Frank Sargent
Salmonella enterica serovar Typhimurium has the ability to use molecular hydrogen as a respiratory electron donor. This is facilitated by three [NiFe]-hydrogenases termed Hyd-1, Hyd-2 and Hyd-5. Hyd-1 and Hyd-5 are homologous oxygen-tolerant [NiFe]-hydrogenases. A critical step in the biosynthesis of a [NiFe]-hydrogenase is the proteolytic processing of the catalytic subunit. In this work, the role of the maturation protease encoded within the Hyd-5 operon, HydD, was found to be partially complemented by the maturation protease encoded in the Hyd-1 operon, HyaD...
December 28, 2016: FEBS Letters
https://www.readbyqxmd.com/read/28027394/ire1%C3%AE-nucleotide-sequence-cleavage-specificity-in-the-unfolded-protein-response
#19
LETTER
Juthakorn Poothong, Pattarawut Sopha, Randal J Kaufman, Witoon Tirasophon
Inositol requiring enzyme 1 (IRE1) is a conserved sensor of the unfolded protein response (UPR) that has protein kinase and endoribonuclease (RNase) enzymatic activities and thereby initiates HAC1/XBP1 splicing. Previous studies demonstrated that human IRE1α (hIRE1α) does not cleave S. cerevisiae HAC1 mRNA. Using an in vitro cleavage assay, we show that adenine to cytosine nucleotide substitution at the +1 position in the 3' splice site of HAC1 RNA is required for specific cleavage by hIRE1α. A similar restricted nucleotide specificity in the RNA substrate was observed for XBP1 splicing in vivo...
December 27, 2016: FEBS Letters
https://www.readbyqxmd.com/read/28027393/the-self-association-of-hmgb1-and-its-possible-role-in-the-binding-to-dna-and-cell-membrane-receptors
#20
REVIEW
Wresti L Anggayasti, Ricardo L Mancera, Steve Bottomley, Erik Helmerhorst
HMGB1, a chromatin protein, interacts with DNA and controls gene expression. However, when HMGB1 is released from apoptotic or damaged cells it triggers pro-inflammatory reactions by interacting with various receptors, mainly RAGE and TLRs. The self-association of HMGB1 has been found to be crucial for its DNA-related biological functions. It is influenced by several factors, such as ionic strength, pH, specific divalent metal cations, redox environment and acetylation. This self-association may also play a role in the interaction with RAGE and TLRs and the concomitant inflammatory responses...
December 27, 2016: FEBS Letters
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