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FEBS Letters

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https://www.readbyqxmd.com/read/28194753/interaction-between-rnf8-and-dyrk2-is-required-for-the-recruitment-of-dna-repair-molecules-to-dna-double-strand-breaks
#1
LETTER
Takenori Yamamoto, Naoe Taira Nihira, Satomi Yogosawa, Katsuhiko Aoki, Hiroyuki Takeda, Tatsuya Sawasaki, Kiyotsugu Yoshida
The genome of eukaryotic cells is frequently exposed to damage by various genotoxins. Phosphorylation of histone H2AX at Serine 139 (γ-H2AX) is a hallmark of DNA damage. RNF8 monoubiquitinates γ-H2AX with the Lys63-linked ubiquitin chain to tether DNA repair molecules at DNA lesions. A high-throughput screening identified RNF8 as a binding partner of dual specificity tyrosine phosphorylation-regulated kinase 2 (DYRK2). Notably, DNA damage-induced monoubiquitination of γ-H2AX is impaired in DYRK2-depleted cells...
February 14, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28192603/characterizing-isomir-variants-within-the-microrna-34-449-family
#2
LETTER
Olivier Mercey, Alexandra Popa, Amélie Cavard, Agnès Paquet, Benoît Chevalier, Nicolas Pons, Virginie Magnone, Joséphine Zangari, Patrick Brest, Laure-Emmanuelle Zaragosi, Gilles Ponzio, Kevin Lebrigand, Pascal Barbry, Brice Marcet
MiR-34/449 microRNAs are conserved regulators of multiciliated cell differentiation. Here, we evidence and characterize expression of two isomiR variant sequences from the miR-34/449 family in human airway epithelial cells. These isomiRs differ from their canonical counterparts miR-34b and miR-449c by one supplemental uridine at their 5'-end, leading to a one base shift in their seed region. Overexpression of canonical miR-34/449 or 5'-isomiR-34/449 induces distinct gene expression profiles and biological effects...
February 13, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28186641/dioleoyl-phosphatidic-acid-selectively-binds-to-%C3%AE-synuclein-and-strongly-induces-its-aggregation
#3
LETTER
Satoru Mizuno, Hirotaka Sasai, Aiko Kume, Daisuke Takahashi, Mamoru Satoh, Sayaka Kado, Fumio Sakane
α-Synuclein (α-syn), which causally links to Parkinson's disease, binds to vesicles containing phosphatidic acid (PA). However, the effects of the fatty acyl chains of PA on its ability to bind to α-syn protein remain unclear. Intriguingly, we reveal that among several PA species, 18:1/18:1-PA is the most strongly bound PA to the α-syn protein. Moreover, 18:1/18:1-PA more strongly enhances secondary structural changes from the random coil form to the α-helical form than 16:0/18:1-PA. Furthermore, 18:1/18:1-PA more markedly accelerates generation of multimeric and proteinase K-resistant α-syn protein compared to 16:0/18:1-PA...
February 10, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28186640/insights-into-the-formation-of-carlactone-from-in-depth-analysis-of-the-ccd8-catalyzed-reactions
#4
Mark Bruno, Martina Vermathen, Adrian Alder, Florian Wüst, Patrick Schaub, Rob van der Steen, Peter Beyer, Sandro Ghisla, Salim Al-Babili
Strigolactones (SLs) are a new class of phytohormones synthesized from carotenoids via carlactone. The complex structure of carlactone is not easily deducible from its precursor, a cis-configured β-carotene cleavage product, and is thus formed via a poorly understood series of reactions and molecular rearrangements, all catalyzed by only one enzyme, the carotenoid cleavage dioxygenase 8 (CCD8). Moreover, the reactions leading to carlactone are expected to form a second, yet unidentified product. In this study, we used (13) C and (18) O-labelling to shed light on the reactions catalyzed by CCD8...
February 10, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28186340/altered-lysosome-distribution-is-an-early-neuropathological-event-in-neurological-forms-of-gaucher-disease
#5
LETTER
Hila Zigdon, Anna Meshcheriakova, Tamar Farfel-Becker, Giora Volpert, Helena Sabanay, Anthony H Futerman
In the lysosomal storage disorder Gaucher disease, glucosylceramide (GlcCer) accumulates due to the defective activity of glucocerebrosidase. A subset of Gaucher disease patients develop neuropathology. We now show mislocalization of Limp2-positive puncta and a large reduction in the number of Lamp1-positive puncta, which are associated with impaired tubulin. These changes occur at an early stage in animal models of Gaucher disease, prior to development of overt symptoms and considerably earlier than neuronal loss...
February 10, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28186333/the-exponential-growth-of-autophagy-related-research-from-the-humble-yeast-to-the-nobel-prize
#6
Noboru Mizushima
Autophagy was discovered more than half a century ago. In the early days, autophagy was studied mostly through the use of biochemical methods and electron microscopy. In the 1990s, yeast genetics was introduced to this field and brought about an exponential expansion. The 2016 Nobel Prize in Physiology or Medicine was eventually awarded to the scientist who spearheaded the rapid development of the field: Yoshinori Ohsumi. Here, I describe in a Nutshell how the autophagy machinery was discovered and how the autophagy research field has grown following the breakthroughs from yeast studies...
February 10, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28186330/exploring-the-mechanism-of-inhibition-of-human-telomerase-by-cysteine-reactive-compounds
#7
LETTER
Guillaume Kellermann, Florent Dingli, Vanessa Masson, Daniel Dauzonne, Evelyne Ségal-Bendirdjian, Marie-Paule Teulade-Fichou, Damarys Loew, Sophie Bombard
Telomerase is an almost universal cancer target that consists minimally of a core protein (hTERT) and an RNA (hTR). Some inhibitors of this enzyme are thought to function by the covalent binding to one or several cystein residues; however, this inhibition mechanism has never been investigated because of the difficulty in producing telomerase. In the present study, we use a recent method to produce recombinant hTERT to analyse the effect of cysteine reactive inhibitors on telomerase. Using mass-spectrometry (MS) and mutagenesis analysis, we identify several targeted residues in separated domains of the hTERT protein and show that cysteine-reactive reagents abolish the interaction with the CR4/5 region of hTR...
February 10, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28185264/amyloidogenicity-and-toxicity-of-the-reverse-and-scrambled-variants-of-amyloid-%C3%AE-1-42
#8
Devkee M Vadukul, Oyinkansola Gbajumo, Karen E Marshall, Louise C Serpell
β-amyloid 1-42 (Aβ1-42) is a self-assembling peptide that goes through many conformational and morphological changes before forming the fibrils that are deposited in extracellular plaques characteristic of Alzheimer's disease. The link between Aβ1-42 structure and toxicity is of major interest, in particular, the neurotoxic potential of oligomeric species. Many studies utilise reversed (Aβ42-1) and scrambled (AβS) forms of amyloid-β as control peptides. Here, using circular dichroism, Thioflavin T fluorescence and transmission electron microscopy, we reveal that both control peptides self-assemble to fibres within 24 hours...
February 10, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28173622/osteoclast-precursors-do-not-express-cd68-results-from-cd68-promoter-driven-rank-transgenic-mice
#9
Melissa F Jackson, Marta Scatena, Cecilia M Giachelli
Macrophages and osteoclasts are thought to derive from CD68 lineage marker-positive common myeloid precursors. We used the CD68 promoter to drive an inducible receptor activator of NF-κB (iRANK) construct that selectively activates RANK signaling in myeloid cells in vivo. The cytoplasmic portion of RANK was fused to a mutant FK506 binding domain, which selectively binds the chemical inducer of dimerization AP20187 and initiates signaling. iRANK mRNA was expressed in macrophages isolated from peritoneal cavity, spleen and bone marrow-derived myeloid cells...
February 7, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28173615/mlf1ip-promotes-normal-erythroid-proliferation-and-is-involved-in-the-pathogenesis-of-polycythemia-vera
#10
LETTER
Gege Feng, Tianjiao Zhang, Jinqin Liu, Xiaotang Ma, Bing Li, Lin Yang, Yue Zhang, Zefeng Xu, Tiejun Qin, Jiaxi Zhou, Gang Huang, Lihong Shi, Zhijian Xiao
Myelodysplasia/myeloid leukemia factor 1-interacting protein (MLF1IP) appears to be an erythroid lineage-specific gene in mice; however, its role in normal erythropoiesis and erythropoietic disorders have not yet been elucidated. Here, we found that MLF1IP is abundantly expressed in human erythroid progenitor cells and that MLF1IP-deficiency reduces cell proliferation resulting from cell cycle arrest. Moreover, MLF1IP expression is exclusively elevated in CFU-E cells from polycythemia vera (PV) patients, and MLF1IP transgenic mice develop a PV-like disorder...
February 7, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28166363/elucidating-the-role-of-the-plg72-r30k-substitution-in-schizophrenia-susceptibility
#11
LETTER
Silvia Sacchi, Pamela Cappelletti, Luciano Pirone, Giovanni Smaldone, Emilia Pedone, Loredano Pollegioni
In the human brain, pLG72 interacts with the flavoenzyme D-amino acid oxidase (hDAAO), which is involved in catabolism of D-serine, a co-agonist of N-methyl-D-aspartate receptors. Here, we investigated the wild-type pLG72, the R30K variant associated with schizophrenia susceptibility, and the K62E variant. The protein conformation, oligomeric state, ligand- and hDAAO-binding properties are only slightly modified by the substitutions. All pLG72 variants inhibit hDAAO and lead to an increase in cellular (D/D+L)-serine...
February 6, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28155233/molecular-basis-for-tank-recognition-by-traf1-revealed-by-the-crystal-structure-of-traf1-tank-complex
#12
LETTER
Chang Min Kim, Jae-Hee Jeong, Young-Jin Son, Jun-Hyuk Choi, Sunghwan Kim, Hyun Ho Park
Tumor necrosis factor receptor-associated factor 1 (TRAF1) is a multi-functional adaptor protein involved in important processes of cellular signaling, including innate immunity and apoptosis. TRAF family member-associated NF-kappaB activator (TANK) has been identified as a competitive intracellular inhibitor of TRAF2 function. Although TRAF recognition by various receptors has been studied extensively in the field of TRAF-mediated biology, molecular and functional details of TANK recognition and interaction with TRAF1 have not been studied...
February 2, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28155229/processing-of-pats-a-morphogen-precursor-in-cell-extracts-of-anabaena-sp-pcc-7120
#13
LETTER
Lianglin Zhang, Fang Zhou, Shuai Wang, Xudong Xu
Upon N-stepdown, Anabaena sp. PCC 7120 differentiates heterocysts along filaments in a semi-regular pattern. A 17-amino acid peptide called PatS is a morphogen precursor for pattern formation. The principal PatS derivative involved in heterocyst patterning has been proposed to be the C-terminal peptide PatS-5 (RGSGR), PatS-6 (ERGSGR) or PatS-8 (CDERGSGR). We present the first evidence for processing of PatS in cell extracts of this cyanobacterium. PatS is probably cleaved between the C-terminal 7th and 8th amino acid residues, producing PatS-7 (DERGSGR), then converted into PatS-6 and PatS-5...
February 2, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28155219/recg-controls-dna-amplification-at-double-strand-breaks-and-arrested-replication-forks
#14
REVIEW
Benura Azeroglu, David Leach
DNA amplification is a powerful mutational mechanism that is a hallmark of cancer and drug resistance. It is therefore important to understand the fundamental pathways that cells employ to avoid over-replicating sections of their genomes. Recent studies demonstrate that, in the absence of RecG, DNA amplification is observed at sites of DNA double-strand break repair (DSBR) and of DNA replication arrest that are processed to generate double-strand ends. RecG also plays a role in stabilising joint molecules formed during DSBR...
February 2, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28144940/production-of-alkenes-and-novel-secondary-products-by-p450-oletje-using-novel-h2-o2-generating-fusion-protein-systems
#15
LETTER
Sarah Matthews, Kang Lan Tee, Nicholas J Rattray, Kirsty J McLean, David Leys, David A Parker, Richard T Blankley, Andrew W Munro
Jeotgalicoccus sp. 8456 OleTJE (CYP152L1) is a fatty acid decarboxylase cytochrome P450 that uses hydrogen peroxide (H2 O2 ) to catalyse production of terminal alkenes, which are industrially important chemicals with biofuel applications. We report enzyme fusion systems in which Streptomyces coelicolor alditol oxidase (AldO) is linked to OleTJE . AldO oxidizes polyols (including glycerol), generating H2 O2 as a co-product and facilitating its use for efficient OleTJE -dependent fatty acid decarboxylation. AldO activity is regulatable by polyol substrate titration, enabling control over H2 O2 supply to minimise oxidative inactivation of OleTJE and prolong activity for increased alkene production...
February 1, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28140449/the-deubiquitinating-enzyme-usp5-regulates-notch-and-rtk-signaling-during-drosophila-eye-development
#16
LETTER
Xuemei Ling, Qinzhu Huang, Yanqin Xu, Yuxiao Jin, Ying Feng, Weijie Shi, Xiaolei Ye, Yi Lin, Ling Hou, Xinhua Lin
Usp5 belongs to the USP family of deubiquitinating enzymes (DUBs), which comprises the largest class of DUBs. We previously reported that loss of Usp5 impairs development of photoreceptors in Drosophila eyes, but the detailed mechanism remained unclear. In this study, we demonstrate that Usp5 regulates both Notch and receptor tyrosine kinase (RTK) signaling. Loss of Usp5 results in upregulation of Notch signaling and downregulation of RTK signaling, leading to impaired photoreceptor development. Moreover, genetic rescue experiments with Su(H) or Notch RNAi indicate that they mediate the regulation of RTK signaling by Usp5...
January 31, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28134446/cesium-reversibly-suppresses-hela-cell-proliferation-by-inhibiting-cellular-metabolism
#17
LETTER
Daisuke Kobayashi, Kei Kakinouchi, Tomoki Nagae, Toshihiko Nagai, Kiyohito Shimura, Akihiro Hazama
The aim of the present study was to investigate the influence of Cs(+) on cultured human cells. We find that HeLa cell growth is suppressed by the addition of 10 mM CsCl into the culture media. In the Cs(+) -treated cells, the intracellular Cs(+) and K(+) concentrations are increased and decreased, respectively. This leads to a decrease in activity of the glycolytic enzyme pyruvate kinase, which uses K(+) as a cofactor. Cs(+) -treated cells show an intracellular pH shift towards alkalization. Based on these results, CsCl presumably suppresses HeLa cell proliferation by inducing an intracellular cation imbalance that affects cell metabolism...
January 30, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28130884/the-pufx-quinone-channel-enables-the-lh1-antenna-to-bind-more-carotenoids-for-light-collection-and-photoprotection
#18
LETTER
John D Olsen, Elizabeth C Martin, C Neil Hunter
Photosynthesis in some phototrophic bacteria requires the PufX component of the reaction centre-light-harvesting 1-PufX (RC-LH1-PufX) complex, which creates a pore for quinone/quinol (Q/QH2 ) exchange across the LH1 barrier surrounding the RC. However, photosynthetic bacteria such as Thermochromatium (T.) tepidum do not require PufX because there are fewer carotenoid binding sites, creating multiple pores in the LH1 ring for Q/QH2 exchange. We show that an αTrp-24 →Phe alteration of the Rhodobacter (Rba...
January 28, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28130840/investigating-the-nucleic-acid-interactions-of-histone-derived-antimicrobial-peptides
#19
Sukin Sim, Penny Wang, Brittany N Beyer, Kara J Cutrona, Mala L Radhakrishnan, Donald E Elmore
While many antimicrobial peptides (AMPs) disrupt bacterial membranes, some translocate into bacteria and interfere with intracellular processes. Buforin II and DesHDAP1 are thought to kill bacteria by interacting with nucleic acids. Here, molecular modeling and experimental measurements are used to show that neither nucleic acid binding peptide selectively binds DNA sequences. Simulations and experiments also show that changing lysines to arginines enhances DNA binding, suggesting that including additional guanidinium groups is a potential strategy to engineer more potent AMPs...
January 28, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28130831/identification-of-amino-acid-residues-important-for-the-arsenic-resistance-function-of-arabidopsis-abcc1
#20
LETTER
Jie Zhang, Jae-Ung Hwang, Won-Yong Song, Enrico Martinoia, Youngsook Lee
The Arabidopsis ATP-Binding Cassette (ABC) transporter ABCC1 sequesters arsenic (As)-phytochelatin conjugates into the vacuole, thereby conferring As resistance. Here, we report the results of a screen for phosphorylation-dependent regulation sites of AtABCC1. Variants of AtABCC1 harboring mutations that replaced amino acid residues Tyr(682) , Tyr(709) , Tyr(822) , Ser(846) , Ser(1278) , or Thr(1408) with alanine confer reduced resistance and decrease the intracellular As content relative to wild-type AtABCC1 when heterologously expressed in the SM7 yeast strain...
January 28, 2017: FEBS Letters
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