journal
MENU ▼
Read by QxMD icon Read
search

FEBS Letters

journal
https://www.readbyqxmd.com/read/28440865/generation-of-wavy-structure-on-lipid-membrane-by-peripheral-proteins-a-linear-elastic-analysis
#1
Paritosh Mahata, Sovan Lal Das
We carry out a linear elastic analysis to study wavy structure generation on lipid membrane by peripheral membrane proteins. We model the lipid membrane as linearly elastic and anisotropic material. The hydrophobic insertion by proteins into the lipid membrane has been idealized as penetration of rigid rodlike inclusions into the membrane and the electrostatic interaction between protein and membrane has been modeled by a distributed surface traction acting on the membrane surface. With the proposed model we study curvature generation by several binding domains of peripheral membrane proteins containing BAR domains and amphipathic alpha-helices...
April 25, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28423184/the-differentiated-and-conserved-roles-of-swi5-sfr1-in-homologous-recombination
#2
REVIEW
Bilge Argunhan, Yasuto Murayama, Hiroshi Iwasaki
Homologous recombination (HR) is the process whereby two DNA molecules that share high sequence similarity are able to recombine to generate hybrid DNA molecules. Throughout evolution, the ability of HR to identify highly similar DNA sequences has been adopted for numerous biological phenomena including DNA repair, meiosis, telomere maintenance, ribosomal DNA amplification, and immunological diversity. Although Rad51 and Dmc1 are the key proteins that promote HR in mitotic and meiotic cells, respectively, accessory proteins that allow Rad51 and Dmc1 to effectively fulfil their functions have been identified in all examined model systems...
April 19, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28423182/crystal-structure-of-the-envz-periplasmic-domain-with-chaps
#3
LETTER
Eunha Hwang, Hae-Kap Cheong, Sang-Yoon Kim, Ohsuk Kwon, Katherine Y Blain, Senyon Choe, Kwon Joo Yeo, Yong Woo Jung, Young Ho Jeon, Chaejoon Cheong
Bacteria sense and respond to osmolarity through the EnvZ-OmpR two-component system. The structure of the periplasmic sensor domain of EnvZ (EnvZ-PD) is not available yet. Here, we present the crystal structure of EnvZ-PD in the presence of CHAPS detergent. The structure of EnvZ-PD shows similar folding topology to the PDC domains of PhoQ, DcuS, and CitA, but distinct orientations of helices and β-hairpin structures. The CD and NMR spectra of EnvZ-PD in the presence of cholate, a major component of bile salts, are similar to those with CHAPS...
April 19, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28423180/mir-19-regulates-breast-cancer-cell-aggressiveness-by-targeting-profilin-1
#4
LETTER
Jingjing Zhou, Xiaoxue Zhang, Haihui Shi, Chunsun Fan
MicroRNAs (miRNAs) have emerged as novel regulators of cancer genes. Here, we identify profilin 1 (Pfn1) as a miR-19 target gene. Pfn1 and miR-19 levels are inversely correlated in breast cancer cell lines. Overexpression of miR-19 reduces endogenous Pfn1 and promotes migration and invasion of MCF-7 cells. Inhibition of miR-19 reduces migration and invasion of MDA-MB-231 cells, which could be partly recovered by transfection of a specific Pfn1 siRNA. Furthermore, miR-19 expression is much higher in grade 3 than in grade 1 breast cancer tissues...
April 19, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28423179/glucan-synthase-like-2-is-indispensable-for-paramylon-synthesis-in-euglena-gracilis
#5
LETTER
Yuji Tanaka, Takahisa Ogawa, Takanori Maruta, Yuta Yoshida, Kazuharu Arakawa, Takahiro Ishikawa
The phytoflagellate Euglena gracilis produces a large amount of paramylon, a conglomerate of liner β-1,3-glucan chains, as a storage polysaccharide. Paramylon is synthesized from UDP-glucose, but its mechanism of formation is largely unknown. Two enzymes, glucan synthase-like (EgGSL) 1 and EgGSL2 were previously identified as candidates for paramylon synthesis in a Euglena transcriptome analysis. Here, we performed a reverse genetic analysis on these enzymes. Knockdown of EgGSL2, but not EgGSL1, significantly inhibits paramylon accumulation in Euglena cells...
April 19, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28417461/2-keto-3-deoxy-l-galactonate-mediates-the-induction-of-genes-involved-in-d-galacturonic-acid-utilization-in-aspergillus-niger
#6
LETTER
Ebru Alazi, Claire Khosravi, Tim G Homan, Saskia du Pré, Mark Arentshorst, Marcos Di Falco, Thi T M Pham, Mao Peng, Maria Victoria Aguilar-Pontes, Jaap Visser, Adrian Tsang, Ronald P de Vries, Arthur F J Ram
In Aspergillus niger, the enzymes encoded by gaaA, gaaB and gaaC catabolize D-galacturonic acid (GA) consecutively into L-galactonate, 2-keto-3-deoxy-L-galactonate, pyruvate and L-glyceraldehyde, while GaaD converts L-glyceraldehyde to glycerol. Deletion of gaaB or gaaC results in severely impaired growth on GA and accumulation of L-galactonate and 2-keto-3-deoxy-L-galactonate, respectively. Expression levels of GA-responsive genes are specifically elevated in the ∆gaaC mutant on GA as compared to the reference strain and other GA catabolic pathway deletion mutants...
April 18, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28417458/resistin-causes-g1-arrest-in-colon-cancer-cells-through-upregulation-of-socs3
#7
LETTER
Snahlata Singh, Surbhi Chouhan, Naoshad Mohammad, Manoj Kumar Bhat
Resistin, a pro-inflammatory cytokine, is elevated in a number of pathological disorders, including cancer. The serum resistin level in colon cancer patients is elevated and correlates with tumor grade. However, the implications of increased resistin on colon cancer cells remain unclear. In the present study, we find that resistin binds to TLR4 on colon cancer cell membranes and initiates TLR4-MyD88-dependent activation of ERK. In addition, the upregulation of SOCS3 by ERK downregulates the JAK2/STAT3 pathway and causes the arrest of cells in G1 phase...
April 18, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28407231/an-inhibitory-rna-aptamer-against-the-lambda-ci-repressor-shows-transcriptional-activator-activity-in-vivo
#8
LETTER
Shoji Ohuchi, Beatrix Suess
An RNA aptamer is one of the promising components for constructing artificial genetic circuits. In this study, we developed a transcriptional activator based on an RNA aptamer against one of the most frequently applied repressor proteins, lambda phage cI. In vitro selection (SELEX), followed by in vivo screening identified an RNA aptamer with the intended transcriptional activator activity from an RNA pool containing a 40-nucleotide long random region. Quantitative analysis showed 35-fold elevation of reporter expression upon aptamer expression...
April 13, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28391641/promiscuity-in-post-transcriptional-control-of-gene-expression-drosophila-sex-lethal-and-its-regulatory-partnerships
#9
REVIEW
Rebecca Moschall, Monika Gaik, Jan Medenbach
The Drosophila RNA-binding protein Sex-lethal (Sxl) is a potent post-transcriptional regulator of gene expression that controls female development. It regulates the expression of key factors involved in sex-specific differences in morphology, behaviour, and dosage compensation. Functional Sxl protein is only expressed in female flies, where it binds to U-rich RNA motifs present in its target mRNAs to regulate their fate. Sxl is a very versatile regulator that, by shuttling between the nucleus and the cytoplasm, can regulate almost all aspects of post-transcriptional gene expression including RNA processing, nuclear export, and translation...
April 9, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28391640/detecting-the-significant-flux-backbone-of-escherichia-coli-metabolism
#10
LETTER
Oriol Güell, Francesc Sagués, M Ángeles Serrano
The heterogeneity of computationally predicted reaction fluxes in metabolic networks within a single flux state can be exploited to detect their significant flux backbone. Here, we disclose the backbone of Escherichia coli, and compare it with the backbones of other bacteria. We find that, in general, the core of the backbones is mainly composed of reactions in energy metabolism corresponding to ancient pathways. In E. coli, the synthesis of nucleotides and the metabolism of lipids form smaller cores which rely critically on energy metabolism...
April 9, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28391636/impaired-hypoxic-tolerance-in-app23-mice-a-dysregulation-of-neuroprotective-globin-levels
#11
LETTER
Zoë P Van Acker, Evi Luyckx, Wendy Van Leuven, Eva Geuens, Peter P De Deyn, Debby Van Dam, Sylvia Dewilde
Though neuroglobin confers neuroprotection against Alzheimer's disease (AD) pathology, its expression becomes downregulated in late-stage AD. Here, we provide evidence that indicates that this decrease is associated with the AD-linked angiopathy. While wild-type mice of different ages show upregulated cerebral neuroglobin expression upon whole-body hypoxia, APP23 mice exhibit decreased cerebral transcription of neuroglobin. Interestingly, transcription of cytoglobin, whose involvement in amyloid pathology still needs to be elucidated, follows a similar pattern...
April 9, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28391617/identification-and-three-dimensional-structure-of-carnobacteriocin-xy-a-class-iib-bacteriocin-produced-by-carnobacteria
#12
LETTER
Jeella Z Acedo, Kaitlyn M Towle, Christopher T Lohans, Mark Miskolzie, Ryan T McKay, Thomas A Doerksen, John C Vederas, Leah A Martin-Visscher
In this study, we report that CbnX (33 residues) and CbnY (29 residues) comprise a class IIb (two-component) bacteriocin in Carnobacteria. Individually, CbnX and CbnY are inactive, but together act synergistically to exert a narrow spectrum of activity. The structures of CbnX and CbnY in structure-inducing conditions were determined and strongly resemble other class IIb bacteriocins (i.e. LcnG, PlnEF, PlnJK). CbnX has an extended, amphipathic α-helix and a flexible C-terminus. CbnY has two α-helices (one hydrophobic, one amphipathic) connected by a short loop and a cationic C-terminus...
April 9, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28391595/human-mettl12-is-a-mitochondrial-methyltransferase-that-modifies-citrate-synthase
#13
LETTER
Virginie F Rhein, Joe Carroll, Shujing Ding, Ian M Fearnley, John E Walker
The protein methylome in mammalian mitochondria has been little studied until recently. Here, we describe that lysine-368 of human citrate synthase is methylated and that the modifying enzyme, localized in the mitochondrial matrix, is methyltransferase-like protein 12 (METTL12), a member of the family of 7β-strand methyltransferases. Lysine-368 is near the active site of citrate synthase, but removal of methylation has no effect on its activity. In mitochondria, it is possible that some or all of the enzymes of the citric acid cycle, including citrate synthase, are organized in metabolons to facilitate channeling of substrates between participating enzymes...
April 9, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28380258/an-essential-role-of-intestinal-cell-kinase-in-lung-development-is-linked-to-the-perinatal-lethality-of-human-eco-syndrome
#14
LETTER
Yixin Tong, So Hyun Park, Di Wu, Wenhao Xu, Stacey J Guillot, Li Jin, Xudong Li, Yalin Wang, Chyuan-Sheng Lin, Zheng Fu
Human endocrine-cerebro-osteodysplasia (ECO) syndrome, caused by the loss-of-function mutation R272Q in the intestinal cell kinase (ICK) gene, is a neonatal-lethal developmental disorder. To elucidate the molecular basis of ECO syndrome, we constructed an Ick R272Q knock-in mouse model that recapitulates ECO pathological phenotypes. Newborns bearing Ick R272Q homozygous mutations die at birth due to respiratory distress. Ick mutant lungs exhibit not only impaired branching morphogenesis associated with reduced mesenchymal proliferation but also significant airspace deficiency in primitive alveoli concomitant with abnormal interstitial mesenchymal differentiation...
April 5, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28380257/tdp-43-and-fus-en-route-from-the-nucleus-to-the-cytoplasm
#15
REVIEW
Helena Ederle, Dorothee Dormann
Misfolded or mislocalized RNA-binding proteins (RBPs) and, consequently, altered mRNA processing, can cause neuronal dysfunction, eventually leading to neurodegeneration. Two prominent examples are the RBPs TAR DNA-binding protein of 43 kDa (TDP-43) and fused in sarcoma (FUS), which form pathological messenger ribonucleoprotein aggregates in patients suffering from amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), two devastating neurodegenerative disorders. Here, we review the multiple functions of TDP-43 and FUS in mRNA processing, both in the nucleus and in the cytoplasm...
April 5, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28380254/molecular-characterization-of-lysyl-oxidase-mediated-extracellular-matrix-remodeling-during-mouse-decidualization
#16
LETTER
Shu-Yun Li, Yan Jiaqi, Zhuo Song, Yue-Fang Liu, Min-Jie Song, Jia-Wen Qin, Zeng-Ming Yang, Xiao-Huan Liang
The establishment of decidualization is a prerequisite of successful pregnancy. Lysyl oxidase (Lox) is a copper-containing amine oxidase which catalyzes cross-linking of collagen and elastin in the ECM. Lox is expressed in the subluminal stroma surrounding the implanting blastocyst on day 5 of pregnancy. From days 6 to 8, the signals for Lox mRNA and protein are strongly detected in the decidual cells. The expression of Lox is under the control of estrogen via the GSK-3β/β-catenin/c-myc pathway. Dtprp is decreased by the inhibition of Lox activity...
April 5, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28380253/structural-analysis-of-sgvp-involved-in-carbon-sulfur-bond-formation-during-griseoviridin-biosynthesis
#17
LETTER
Qin Li, Yan Chen, Guiqin Zhang, Huaidong Zhang
Griseoviridin is a broad-spectrum antibiotic with antibacterial and antifungal activity. In the griseoviridin biosynthetic pathway, SgvP catalyzes formation of the carbon-sulfur bond in griseoviridin. Herein we report the recombinant expression and characterization of SgvP from Streptomyces griseoviridis NRRL2427. We also present the 2.6 Å crystal structure of SgvP, which is the first structure of a cytochrome P450 involved in carbon-sulfur bond formation in griseoviridin. Structural analysis indicates that Pro237 in the I-helix of SgvP may play a critical role in dioxygen binding and proton transfer during the catalytic cycle...
April 5, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28376244/structural-communication-between-the-chromophore-binding-pocket-and-the-n-terminal-extension-in-plant-phytochrome-phyb
#18
LETTER
Francisco Velázquez Escobar, David Buhrke, Maria Fernandez Lopez, Sintayehu Manaye Shenkutie, Silke von Horsten, Lars-Oliver Essen, Jon Hughes, Peter Hildebrandt
The N-terminal extension (NTE) of plant phytochromes has been suggested to play a functional role in signaling photoinduced structural changes. Here, we use resonance Raman spectroscopy to study the effect of the NTE on the chromophore structure of B-type phytochromes from two evolutionarily distant plants. NTE deletion seems to have no effect on the chromophore in the inactive Pr state, but alters the torsion of the C-D ring methine bridge and the surrounding hydrogen bonding network in the physiologically active Pfr state...
April 4, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28370139/mutations-in-the-tetramer-interface-of-human-glucose-6-phosphate-dehydrogenase-reveals-kinetic-differences-between-oligomeric-states
#19
LETTER
Americo Tavares Ranzani, Artur Torres Cordeiro
Glucose-6-phosphate dehydrogenase (G6PDH) catalyzes the oxidation of glucose-6-phoshate to 6-phospho-gluconolactone with the concomitant reduction of NADP(+) to NADPH. In solution, the recombinant human G6PDH is known to be active as dimers and tetramers. To distinguish between the kinetic properties of dimers and tetramers of the G6PDH is not trivial. Steady-state kinetic experiments are often performed at low enzyme concentrations, which favor the dimeric state. The present work describes two novel human G6PDH mutants, one that creates four disulfide bonds among apposing dimers, resulting in a "cross-linked" tetramer, and another that prevents the dimer to dimer association...
March 30, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28369931/a-farnesoic-acid-responsive-transcription-factor-hot1-regulates-yeast-hypha-morphogenesis-in-candida-albicans
#20
LETTER
Chan-Hong Ahn, Sohyoung Lee, Eunji Cho, Heegyu Kim, Beomkoo Chung, Wanki Park, Shin Jongheon, Ki-Bong Oh
Candida albicans hyphal formation is inhibited by a quorum-sensing molecule, farnesoic acid, which accumulates in the medium as the cells proliferate. We recently showed that Pho81 is essential for the inhibition of hyphal growth by farnesoic acid. Here, we describe a newly identified regulator, Hot1, which increases the expression of PHO81. The binding site of Hot1 in the PHO81 promoter region was identified by DNase I protection assay. The hot1Δ mutant grows extensively as filaments. Furthermore, the inhibition of hyphal formation and the repression of major signaling pathway components in response to farnesoic acid are defective in hot1Δ mutant cells...
March 30, 2017: FEBS Letters
journal
journal
21981
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"