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Acta Histochemica et Cytochemica

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https://www.readbyqxmd.com/read/29622850/expression-of-draxin-in-lung-carcinomas
#1
Younosuke Sato, Akira Matsuo, Shinji Kudoh, Liu Fang, Koki Hasegawa, Yohei Shinmyo, Takaaki Ito
Guidance molecules, such as Netrin-1, and their receptors have important roles in controlling axon pathfinding, modulate biological activities of various cancer cells, and may be a useful target for cancer therapy. Dorsal repulsive axon guidance protein (Draxin) is a novel guidance molecule that binds not only common guidance molecule receptors with Netrin-1, but also directly binds the EGF domain of Netrin-1 through a 22-amino-acid peptide (22aa). By immunostaining, Draxin was positively expressed in small cell carcinoma, adenocarcinoma (ADC), and squamous cell carcinoma of the lung...
February 27, 2018: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29622849/expression-and-dynamics-of-podoplanin-in-cultured-osteoblasts-with-mechanostress-and-mineralization-stimulus
#2
Tomohiro Takenawa, Takenori Kanai, Tetsuya Kitamura, Yoshitaka Yoshimura, Yoshihiko Sawa, Junichiro Iida
This study investigates the significance of the expression and dynamics of podoplanin in mechanostress and mineralization in cultured murine osteoblasts. Podoplanin increased in osteoblasts subjected to straining in non-mineralization medium, suggesting that the mechanostress alone is a podoplanin induction factor. In osteoblasts subjected to vertical elongation straining in the mineralization medium, the mRNA amounts of podoplanin, osteopontin, and osteocalcin were significantly larger than those in cells not subjected to straining, suggesting that mechanostress is the cause of a synergistic effect in the expression of these proteins...
February 27, 2018: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29622848/the-hdac-inhibitor-saha-prevents-colonic-inflammation-by-suppressing-pro-inflammatory-cytokines-and-chemokines-in-dss-induced-colitis
#3
Mohmand Noor Ali, Narantsog Choijookhuu, Hideaki Takagi, Naparee Srisowanna, Mai Nguyen Nhat Huynh, Yuya Yamaguchi, Phyu Synn Oo, Myat Tin Htwe Kyaw, Katsuaki Sato, Ryoji Yamaguchi, Yoshitaka Hishikawa
Inflammatory bowel disease (IBD) is an inflammatory disorder of the gastrointestinal tract that is caused by multiple factors, including dysfunction of the immune system and genetic and epigenetic alterations. Aberrant epigenetic regulation, especially histone acetylation, was found in biopsies from IBD patients and mouse models of colitis, suggesting that an epigenetic treatment approach may be useful for IBD therapy. Therefore, we investigated the effects of the histone deacetylase (HDAC) inhibitor, suberoylanilide hydroxamic acid (SAHA), in a mouse model of dextran sulfate sodium (DSS)-induced colitis...
February 27, 2018: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29622847/estrogen-regulates-mitochondrial-morphology-through-phosphorylation-of-dynamin-related-protein-1-in-mcf7-human-breast-cancer-cells
#4
Phyu Synn Oo, Yuya Yamaguchi, Akira Sawaguchi, Myat Tin Htwe Kyaw, Narantsog Choijookhuu, Mohmand Noor Ali, Naparee Srisowanna, Shin-Ichiro Hino, Yoshitaka Hishikawa
Estrogen affects mitochondrial function in various tissues, but the precise mechanism remains unclear. We, therefore investigated the effect on estrogen-regulated mitochondrial morphology by dynamin-related protein 1 (Drp1) and its Ser616-phosphorylated derivative (pDrp1Ser616 ) are involved in mitochondrial fission. MCF7 human breast cancer cells were treated with 17β-estradiol (E2 ), an estrogen receptor (ER) α and β antagonist (ICI 182, 780), an ERα antagonist (MPP), and an ERβ antagonist (PHTPP) for 24 hr...
February 27, 2018: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29622846/improved-serial-sectioning-techniques-for-correlative-light-electron-microscopy-mapping-of-human-langerhans-islets
#5
Sei Saitoh, Nobuhiko Ohno, Yurika Saitoh, Nobuo Terada, Satoshi Shimo, Kaoru Aida, Hideki Fujii, Tetsuro Kobayashi, Shinichi Ohno
Combined analysis of immunostaining for various biological molecules coupled with investigations of ultrastructural features of individual cells is a powerful approach for studies of cellular functions in normal and pathological conditions. However, weak antigenicity of tissues fixed by conventional methods poses a problem for immunoassays. This study introduces a method of correlative light and electron microscopy imaging of the same endocrine cells of compact and diffuse islets from human pancreatic tissue specimens...
February 27, 2018: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29622845/a-newly-defined-area-of-the-mouse-anterior-hypothalamus-involved-in-septohypothalamic-circuit-perifornical-area-of-the-anterior-hypothalamus-pefah
#6
Noriko Horii-Hayashi, Mayumi Nishi
Although the hypothalamus is classified into more than 10 compartments, it still contains uncharacterized areas. In this study, we identified a new triangular-shaped area between the paraventricular hypothalamic nucleus (PVN) and the fornix area in the mouse anterior hypothalamus, which is enriched in chondroitin sulfate proteoglycans (CSPGs). We designated this region as the perifornical area of the anterior hypothalamus (PeFAH) based on its anatomical location. As evidenced by Nissl staining, the PeFAH was distinguishable as an area of relatively low density...
February 27, 2018: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29343881/validation-of-anti-csp%C3%AE-snap25-tyrosine-hydroxylase-ubiquitin-cleaved-caspase-3-and-pser-pkc-motif-antibodies-for-utilization-in-western-blotting
#7
Toshihiko Shirafuji, Takehiko Ueyama, Shigeru Tanaka, Izumi Hide, Naoaki Saito, Norio Sakai
There are many commercial antibodies with little information provided by their suppliers as to their reliability. Accordingly, commercial antibodies require proper validation before being used in scientific research. In this study, we validated several commercial antibodies, including anti-CSPα, SNAP25, tyrosine hydroxylase, ubiquitin, cleaved caspase 3, and pSer PKC motif. Anti-CSPα, SNAP25, and tyrosine hydroxylase antibodies could detect their endogenous target proteins with some degree of cross-reactivity...
December 26, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29343880/novel-application-of-loop-mediated-isothermal-amplification-for-rapid-detection-of-gene-translocation
#8
Ibu Matsuzaki, Hideto Iguchi, Yurina Mikasa, Hiromu Morishita, Katsuya Okuda, Keita Nakaguchi, Yuki Mori, Yoshifumi Iwahashi, Kenji Warigaya, Masakazu Fujimoto, Fumiyoshi Kojima, Shin-Ichi Murata
Identification of fusion genes in cancer is essential for pathological diagnosis and clinical therapy. Although methods for detection of fusion genes, such as fluorescence in situ hybridization (FISH) and real-time polymerase chain reaction (PCR), have been developed in last two decades, these methods are not ideal for detection of these genetic alterations owing to their high cost and time-consuming procedures. In this study, we developed novel application for detection of gene translocations using loop-mediated isothermal amplification (LAMP)...
December 26, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29343879/ccn3-expression-marks-a-sulfomucin-nonproducing-unique-subset-of-colonic-goblet-cells-in-mice
#9
Shintaro Akiyama, Wakana Mochizuki, Yoichi Nibe, Yuka Matsumoto, Kei Sakamoto, Shigeru Oshima, Mamoru Watanabe, Tetsuya Nakamura
Intestinal goblet cells are characterized by their unique morphology and specialized function to secrete mucins. Although it is known that they are a heterogeneous population of cells, there have been few studies that relate the expression of a particular gene with functionally distinct subpopulations of intestinal goblet cells. Here we show that CCN3, a gene encoding a member of the CCN family proteins, is induced by inhibition of Notch signaling in colonic epithelial cells and expressed in goblet cells in mice...
December 26, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29343878/cxcl14-like-immunoreactivity-exists-in-somatostatin-containing-endocrine-cells-and-in-the-lamina-propria-and-submucosal-somatostatinergic-nervous-system-of-mouse-alimentary-tract
#10
Hirohumi Suzuki, Kentaro Yamada, Yasuhiro Matsuda, Minoru Onozuka, Toshiharu Yamamoto
In the present study, we investigated the distribution of CXCL14 immunoreactive endocrine cells and neurons in mouse alimentary tract by immunohistochemistry. CXCL14 immunoreactive endocrine cells were found as closed-type cells in the stomach and open-type cells in the small intestine. The immunostaining of these endocrine cells corresponded with that of the somatostatin-containing endocrine cells. Only a few CXCL14 immunoreactive endocrine cells were seen in the large intestine. CXCL14 immunoreactive fibers were observed in the muscular layer from the stomach to the rectum with most abundance in the rectum...
December 26, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29276316/a-new-electron-microscopic-method-to-observe-the-distribution-of-phosphatidylinositol-3-4-bisphosphate
#11
Sharmin Aktar, Sho Takatori, Takuma Tsuji, Minami Orii, Yuki Ohsaki, Jinglei Cheng, Toyoshi Fujimoto
Phosphatidylinositol 3,4-bisphosphate [PtdIns(3,4)P2 ] is a phosphoinositide that plays important roles in signal transduction, endocytosis, and cell migration among others. The intracellular distribution of PtdIns(3,4)P2 has mainly been studied by observing the distribution of GFP-tagged PtdIns(3,4)P2 -binding protein domains in live cells and by labeling with anti-PtdIns(3,4)P2 antibody in fixed cell samples, but these methods only offer low spatial resolution results and may have pitfalls. In the present study, we developed an electron microscopic method to observe the PtdIns(3,4)P2 distribution using the SDS-treated freeze-fracture replica labeling method...
October 24, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29276315/a-device-for-ribbon-collection-for-array-tomography-with-scanning-electron-microscopy
#12
Taro Koike, Yosky Kataoka, Mitsuyo Maeda, Yuji Hasebe, Yuuki Yamaguchi, Mitsuo Suga, Akira Saito, Hisao Yamada
"Array tomography" is a method used to observe the fine structure of cells and tissues in a three-dimensional view. In this method, serial ultrathin sections in the ribbon state (ribbons) are mounted on a solid substrate and observed by scanning electron microscopy (SEM). The method may also be used in conjunction with post-embedding immunocytochemistry. However, it is difficult to mount many serial ribbons on a substrate manually. We developed an inexpensive laboratory-made device that mounts ribbons by pulling a nylon fishing line and lifting the substrate up from the water in a knife boat...
October 24, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/28928542/developmental-contribution-of-wnt-signal-responsive-cells-to-mouse-reproductive-tract-formation
#13
Ryuma Haraguchi, Riko Kitazawa, Aki Murashima, Gen Yamada, Sohei Kitazawa
In mammals, the müllerian duct (MD) is an embryonic tubular structure that gives rise to the female reproductive tract (FRT). The MD originates from the coelomic epithelium (CoE) and takes on a rostral to caudal shape to establish the primary structure of the FRT under the regulation of morphogenetic signals. During these developmental processes, the MD and its derivatives require proper regulation of the Wnt-signaling-pathway. Here, to investigate the developmental contribution of FRT primordia under the influence of the Wnt-signaling, genetic lineage tracing was carried out using TopCreER/Rosa-LacZ mice to follow the fate of Wnt-signal-responsive cells during reproductive tract formation...
August 25, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/28928541/morphological-and-functional-analyses-of-the-tight-junction-in-the-palatal-epithelium-of-mouse
#14
Noriko Shiotsu, Tadafumi Kawamoto, Mariko Kawai, Mika Ikegame, Yasuhiro Torii, Hiroyuki Sasaki, Toshio Yamamoto
Tight junction (TJ) is one of the cell-cell junctions and known to have the barrier and fence functions between adjacent cells in both simple and stratified epithelia. We examined the distribution pattern, constitutive proteins, and permeability of TJ in the stratified squamous epithelium of the palatal mucosa of mice. Ultrastructural observations based on the ultrathin section and freeze-fracture methods revealed that poorly developed TJs are located at the upper layer of the stratum granulosum. The positive immunofluorescence of occludin (OCD), claudin (CLD)-1 and -4 were localized among the upper layer of the stratum granulosum showing a dot-like distribution pattern...
August 25, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/28744028/expression-of-c-kit-and-cytokeratin-5-in-the-submandibular-gland-after-release-of-long-term-ligation-of-the-main-excretory-duct-in-mice
#15
Hiroshi Watanabe, Haruka Takahashi, Miyuki Hata-Kawakami, Akira Tanaka
Long-term submandibular duct obstruction is thought to cause irreversible atrophy and dysfunction of the submandibular gland. As an atrophic gland may be induced clinically by chronic or recurrent infection, it is generally removed surgically. However, the regenerative capacity of atrophic submandibular glands after long-term obstruction is not completely understood. We studied the regenerative capacity of the mouse submandibular duct using an aneurysm clip ligation model. We documented changes in the size, histologic structure and gene expression of the submandibular gland after 2 months of ligation, and 1 week, 1 month and 2 months after clip removal...
June 26, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/28744027/the-effect-of-ultraviolet-b-on-fibrillin-1-and-fibrillin-2-in-human-non-pigmented-ciliary-epithelial-cells-in-vitro
#16
Yuki Shiroto, Shingo Terashima, Yoichiro Hosokawa, Kyoko Oka, Keitaro Isokawa, Eichi Tsuruga
The ciliary zonules link the lens to the ciliary body in the eye, controlling the thickness of the lens for focusing through their characteristic elasticity. The ciliary zonules are composed of oxytalan fibers. Physiological or pathological damage to the ciliary zonules, including exposure to ultraviolet (UV)-A and UV-B components, can lead to lens dislocation. However, no studies have shown whether UV affects the ciliary zonule. Here, we assessed the effects of UV light on human nonpigmented ciliary epithelial cells (HNPCECs)...
June 26, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/28522884/profiles-of-periglomerular-cells-in-the-olfactory-bulb-of-prokineticin-type-2-receptor-deficient-mice
#17
Atsuko Kubo, Mitsugu Sujino, Koh-Hei Masumoto, Atsuko Fujioka, Toshio Terashima, Yasufumi Shigeyoshi, Mamoru Nagano
Both prokineticin receptor 2 (pkr2) and prokineticin 2 (pk2) gene-deficient mice have hypoplasia of the main olfactory bulb (MOB). This hypoplasia has been attributed to disruption of the glomerulus that is caused by loss of afferent projection from olfactory sensory neurons (OSN), and to the impaired migration of granule cells, a type of interneuron. In the present study, we examined whether migration of the second type of interneuron, periglomerular cells (PGC), is dependent on the pkr2 expression by observing the localization of distinct subpopulations of PGC: calretinin (CR)-, calbindin (CB)- and tyrosine hydroxylase (TH)-expressing neurons...
April 27, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/28522883/in-situ-evaluation-of-estrogen-receptor-dimers-in-breast-carcinoma-cells-visualization-of-protein-protein-interactions
#18
Erina Iwabuchi, Yasuhiro Miki, Katsuhiko Ono, Yoshiaki Onodera, Hironobu Sasano
The estrogen receptor (ER) functions as a dimer and is involved in several different biological functions. However ER dimeric proteins have not been identified by in situ methodologies. Structured illumination microscopy (SIM) has been recently developed, which enabled the localization of protein and protein interaction. Therefore, in this study, we firstly demonstrated that ERs formed both homodimers and heterodimers in breast carcinoma cell lines using Nikon's SIM (N-SIM). ERα/α homodimers were detected in the nuclei of both ERα-positive MCF-7 and T-47D cells; 23...
April 27, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/28522882/monoclonal-suncus-antibodies-generation-of-fusion-partners-to-produce-suncus-suncus-hybridomas
#19
Yoshikazu Sado, Satoko Inoue, Yasuko Tomono, Makoto Matsuyama, Masaki Fukushima, Toshitaka Oohashi, Takamichi Jogahara, Sen-Ichi Oda
We used suncus (Suncus murinus; house musk shrew) to generate partner cells for cell fusion to produce suncus monoclonal antibodies. Suncus are insectivores that are genetically distant to rodents, and recognize antigens and epitopes that are not immunogenic in mice and rats, which are the animals most commonly used in basic life science research and from which monoclonal antibodies are usually produced. To date, monoclonal antibodies from suncus have not been generated due to the lack of a plasmacytoma fusion partner...
April 27, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/28522881/notch-signaling-and-maintenance-of-sox2-expression-in-rat-anterior-pituitary-cells
#20
Khongorzul Batchuluun, Morio Azuma, Ken Fujiwara, Takashi Yashiro, Motoshi Kikuchi
After publication of reports describing the presence of stem/progenitor cells among non-hormone-producing cells in the pituitary, the mechanism responsible for proliferation and differentiation generated considerable interest. Several studies have suggested that Notch signaling is involved. In the present study, we examined the histochemical relationship between Notch signaling molecules and the transcription factor SOX2 in rat pituitary. Combined in situ hybridization and immunohistochemistry showed that Notch2 mRNA and SOX2 were co-expressed at embryonic day 14...
April 27, 2017: Acta Histochemica et Cytochemica
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