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Acta Histochemica et Cytochemica

Fumi Nozaki, Yukari Hirotani, Yoko Nakanishi, Hiromi Yamaguchi, Haruna Nishimaki, Hiroko Noda, Xiaoyan Tang, Hisae Yamamoto, Atsuko Suzuki, Toshimi Seki, Shinobu Masuda
p62, also called sequestosome 1 (SQSTM1), is a multifunctional signaling molecule that affects cell proliferation. Recently, we found accumulation of p62 in apocrine carcinoma of the breast, however, the biological role of p62 expression in apocrine carcinoma still remains unclear. To investigate whether p62 might contribute to tumor cell proliferation in apocrine carcinomas, we used the MDA-MB-453 (androgen receptor-positive, HER2-type) and MFM223 (androgen receptor-positive, triple-negative type) breast cancer cell lines as models of molecular apocrine carcinoma...
August 30, 2016: Acta Histochemica et Cytochemica
Takanori Onouchi, Kazuya Shiogama, Takahiro Matsui, Yasuyoshi Mizutani, Kouhei Sakurai, Ken-Ichi Inada, Yutaka Tsutsumi
Neutrophil extracellular traps (NETs) represent an extracellular, spider's web-like structure resulting from cell death of neutrophils. NETs play an important role in innate immunity against microbial infection, but their roles in human pathological processes remain largely unknown. NETs and fibrin meshwork both showing fibrillar structures are observed at the site of fibrinopurulent inflammation, as described in our sister paper [Acta Histochem. Cytochem. 49; 109-116, 2016]. In the present study, immunoelectron microscopic study was performed for visualizing NETs and fibrin fibrils (thick fibrils in our tongue) in formalin-fixed, paraffin-embedded sections of autopsied lung tissue of legionnaire's pneumonia...
August 30, 2016: Acta Histochemica et Cytochemica
Kazuya Shiogama, Takanori Onouchi, Yasuyoshi Mizutani, Kouhei Sakurai, Ken-Ichi Inada, Yutaka Tsutsumi
Neutrophil extracellular traps (NETs) are extracellular fibrillary structures composed of degraded chromatin and granules of neutrophil origin. In fibrinopurulent inflammation such as pneumonia and abscess, deposition of fibrillar eosinophilic material is a common histopathological finding under hematoxylin-eosin staining. Expectedly, not only fibrin fibrils but also NETs consist of the fibrillar material. The aim of the present study is to analyze immunohistochemically how NETs are involved in the inflammatory process...
August 30, 2016: Acta Histochemica et Cytochemica
Gazi Jased Ahmed, Eri Tatsukawa, Kota Morishita, Yasuaki Shibata, Fumio Suehiro, Masanobu Kamitakahara, Taishi Yokoi, Takehiko Koji, Masahiro Umeda, Masahiro Nishimura, Tohru Ikeda
The implantation of biomaterials induces a granulomatous reaction accompanied by foreign body giant cells (FBGCs). The characterization of multinucleated giant cells (MNGCs) around bone substitutes implanted in bone defects is more complicated because of healing with bone admixed with residual bone substitutes and their hybrid, and the appearance of two kinds of MNGCs, osteoclasts and FBGCs. Furthermore, the clinical significance of osteoclasts and FBGCs in the healing of implanted regions remains unclear. The aim of the present study was to characterize MNGCs around bone substitutes using an extraskeletal implantation model and evaluate the clinical significance of osteoclasts and FBGCs...
June 28, 2016: Acta Histochemica et Cytochemica
Minenori Ishido, Tomohiro Nakamura
Aquaporin-4 (AQP4) is a selective water channel that is located on the plasma membrane of myofibers in skeletal muscle and is bound to α1-syntrophin. It is considered that AQP4 is involved in the modulation of homeostasis in myofibers through the regulation of water transport and osmotic pressure. However, it remains unclear whether AQP4 expression is altered by skeletal muscle hypertrophy to modulate water homeostasis in myofibers. The present study investigated the effect of muscle hypertrophy on the changes in AQP4 and α1-syntrophin expression patterns in myofibers...
June 28, 2016: Acta Histochemica et Cytochemica
Surang Chomphoo, Wilaiwan Mothong, Tarinee Sawatpanich, Pipatphong Kanla, Hiroyuki Sakagami, Hisatake Kondo, Wiphawi Hipkaeo
EFA6 (exchange factor for ARF6) activates Arf6 (ADP ribosylation factor 6) by exchanging ADP to ATP, and the resulting activated form of Arf6 is involved in the membrane dynamics and actin re-organization of cells. The present study was attempted to localize EFA6 type D (EFA6D) in mouse adrenocortical cells in situ whose steroid hormone secretion is generally considered not to depend on the vesicle-involved regulatory mechanism. In immunoblotting, an immunoreactive band with the same size as brain EFA6D was detected in homogenates of adrenal cortical tissues almost free of adrenal capsules and medulla...
June 28, 2016: Acta Histochemica et Cytochemica
Katsunori Nochioka, Hiroaki Okuda, Kouko Tatsumi, Shoko Morita, Nahoko Ogata, Akio Wanaka
Choroidal neovascularization is one of the major pathological changes in age-related macular degeneration, which causes devastating blindness in the elderly population. The molecular mechanism of choroidal neovascularization has been under extensive investigation, but is still an open question. We focused on sonic hedgehog signaling, which is implicated in angiogenesis in various organs. Laser-induced injuries to the mouse retina were made to cause choroidal neovascularization. We examined gene expression of sonic hedgehog, its receptors (patched1, smoothened, cell adhesion molecule down-regulated by oncogenes (Cdon) and biregional Cdon-binding protein (Boc)) and downstream transcription factors (Gli1-3) using real-time RT-PCR...
April 28, 2016: Acta Histochemica et Cytochemica
Maosheng Zhan, Yumiko Hori, Naoki Wada, Jun-Ichiro Ikeda, Yuuki Hata, Keigo Osuga, Eiichi Morii
Angiogenic factor with G-patch and FHA domain 1 (AGGF1) is a novel angiogenic factor that was first described in Klippel-Trenaunay syndrome, a congenital vascular disease associated with capillary and venous malformations. AGGF1, similar to vascular endothelial growth factor (VEGF), has been shown to promote strong angiogenesis in chick embryos in vivo. Blocking AGGF1 expression prevented vessel formation, which suggests AGGF1 is a potent angiogenic factor linked to vascular malformations. So far, AGGF1 expression studies in human vascular lesions have not been performed...
April 28, 2016: Acta Histochemica et Cytochemica
Kiyokazu Morioka, Hiromi Takano-Ohmuro
Six isoforms of actins encoded by different genes have been identified in mammals including α-cardiac, α-skeletal, α-smooth muscle (α-SMA), β-cytoplasmic, γ-smooth muscle (γ-SMA), and γ-cytoplasmic actins (γ-CYA). In a previous study we showed the localization of α-SMA and other cytoskeletal proteins in the hairs and their appendages of developing rats (Morioka K., et al. (2011) Acta Histochem. Cytochem. 44, 141-153), and herein we determined the localization of γ type actins in the same tissues and organs by immunohistochemical staining...
April 28, 2016: Acta Histochemica et Cytochemica
Shigeyuki Mukudai, Ken Ichi Matsuda, Hideki Bando, Keiko Takanami, Takeshi Nishio, Yoichiro Sugiyama, Yasuo Hisa, Mitsuhiro Kawata
The medullary vagal motor nuclei, the nucleus ambiguus (NA) and dorsal motor nucleus of the vagus (DMV), innervate the respiratory and gastrointestinal tracts. We conducted immunohistochemical analysis of expression of the androgen receptor (AR) and estrogen receptor α (ERα), in relation to innervation of the trachea and esophagus via vagal motor nuclei in mice. AR and ERα were expressed in the rostral NA and in part of the DMV. Tracing experiments using cholera toxin B subunit demonstrated that neurons of vagal motor nuclei that innervate the trachea and esophagus express AR and ERα...
February 27, 2016: Acta Histochemica et Cytochemica
Maiko Fujii, Hideo Tanaka, Akihiro Nakamura, Chinatsu Suzuki, Yoshinori Harada, Tetsuro Takamatsu, Kenji Hamaoka
Kawasaki disease (KD) is a systemic vasculitis in infants that develops predominantly in the coronary arteries. Despite the clinically transient nature of active inflammation in childhood albeit rare complications (e.g., coronary artery aneurysm), KD has recently been suggested to increase the incidence of ischemic heart diseases in young adulthood. However, little is known about the histopathology of the coronary artery long after development of the acute KD vasculitis. To address this, we conducted histological studies of rabbit coronary arteries in adolescent phase after induction of the KD-like vasculitis induced by horse serum administration...
February 27, 2016: Acta Histochemica et Cytochemica
Erina Ikeda, Tetsuya Goto, Kaori Gunjigake, Kayoko Kuroishi, Masae Ueda, Shinji Kataoka, Takashi Toyono, Mitsushiro Nakatomi, Yuji Seta, Chiaki Kitamura, Tatsuji Nishihara, Tatsuo Kawamoto
Several theories have been proposed regarding pain transmission mechanisms in tooth. However, the exact signaling mechanism from odontoblasts to pulp nerves remains to be clarified. Recently, ATP-associated pain transmission has been reported, but it is unclear whether ATP is involved in tooth pain transmission. In the present study, we focused on the vesicular nucleotide transporter (VNUT), a transporter of ATP into vesicles, and examined whether VNUT was involved in ATP release from odontoblasts. We examined the expression of VNUT in rat pulp by RT-PCR and immunostaining...
February 27, 2016: Acta Histochemica et Cytochemica
Yasuyoshi Mizutani, Kazuya Shiogama, Takanori Onouchi, Kouhei Sakurai, Ken-Ichi Inada, Yutaka Tsutsumi
In chronic inflammatory lesions of autoimmune and infectious diseases, plasma cells are frequently observed. Antigens recognized by antibodies produced by the plasma cells mostly remain unclear. A new technique identifying these corresponding antigens may give us a breakthrough for understanding the disease from a pathophysiological viewpoint, simply because the immunocytes are seen within the lesion. We have developed an enzyme-labeled antigen method for microscopic identification of the antigen recognized by specific antibodies locally produced in plasma cells in inflammatory lesions...
February 27, 2016: Acta Histochemica et Cytochemica
Shinichi Ohno
Recent advances in molecular and genetic techniques have led to establishment of new biomedical fields; however, morphological techniques are still required for a more precise understanding of functioning cells and tissues. Conventional preparation procedures involve a series of chemical fixation, alcohol dehydration, paraffin or epoxy resin embedding, sectioning, and staining steps. In these steps, technical artifacts modify original morphologies of the cells being examined. Furthermore, difficulties are associated with capturing dynamic images in vivo using conventional chemical fixation...
February 27, 2016: Acta Histochemica et Cytochemica
Eun-Shil Lee, Se-Jin Jeong, Yeoun-Hee Kim, Chang-Jin Jeon
The present study aimed to investigate the influence of the host retinal microenvironment on cell migration and differentiation using Neuro2a (N2a) cells transduced with green fluorescent protein. N2a cells were transplanted into the vitreous cavities of developing mouse eyes (C57BL/6) on postnatal days 1, 5, and 10 (P1, 5, and 10). To analyze the effects of the host microenvironment on neural differentiation of N2a cells in vitro, cells were treated with a conditioned medium (CM) collected from retinal cells cultured at each developmental stage...
December 25, 2015: Acta Histochemica et Cytochemica
Seiichi Daikuhara, Takeshi Uehara, Kayoko Higuchi, Noriko Hosaka, Mai Iwaya, Yasuhiro Maruyama, Kazuyuki Matsuda, Norikazu Arakura, Eiji Tanaka, Hiroyoshi Ota
The biological characteristics and roles of insulin-like growth factor II mRNA-binding protein 3 protein (IMP3) expression in small-intestinal adenocarcinoma were investigated. The value of IMP3 immunostaining in the diagnosis of small-intestinal epithelial lesions was also evaluated. Immunohistochemical expression of IMP3 in normal small-intestinal mucosa adjacent to adenoma and adenocarcinoma lesions, and inflamed duodenal and ileal mucosa was analyzed. Samples assessed were: duodenal ulcer (n=6), Crohn's disease (n=5), low-grade small-intestinal adenoma (n=10), high-grade small-intestinal adenoma (n=13), small-intestinal adenocarcinoma (n=23), lymph node metastases (LNM; n=7), and preoperative biopsies of small-intestinal adenocarcinoma (n=6)...
December 25, 2015: Acta Histochemica et Cytochemica
Morio Azuma, Alimuddin Tofrizal, Rita Maliza, Khongorzul Batchuluun, Dini Ramadhani, Rahimi Syaidah, Takehiro Tsukada, Ken Fujiwara, Motoshi Kikuchi, Kotaro Horiguchi, Takashi Yashiro
The extracellular matrix (ECM) is important in creating cellular environments in tissues. Recent studies have demonstrated that ECM components are localized in anterior pituitary cells and affect cell activity. Thus, clarifying the mechanism responsible for ECM maintenance would improve understanding of gland function. Tissue inhibitors of metalloproteinases (TIMPs) are endogenous inhibitors of matrix metalloproteinases and participate in ECM degradation. In this study, we investigated whether cells expressing TIMPs are present in rat anterior pituitary gland...
December 25, 2015: Acta Histochemica et Cytochemica
Norio Iijima, Ken Takumi, Keisuke Matsumoto, Hitoshi Ozawa
The neuropeptide kisspeptin plays an important role in fertility and the onset of puberty, stimulating gonadotropin-releasing hormone (GnRH) neurons to activate the hypothalamic-pituitary-gonadal axis. Several studies have demonstrated a morphological interaction between kisspeptin- and GnRH-expressing neurons; however, few have addressed the interaction of kisspeptin with other neuronal subtypes. We recently showed that fibers immunoreactive for kisspeptin were densely distributed in the dorsal part of the arcuate nucleus...
December 25, 2015: Acta Histochemica et Cytochemica
Hirohumi Suzuki, Toshiharu Yamamoto
Immunohistochemical techniques were employed to investigate the distribution of the chemokine CXCL14, in the mouse pancreas. CXCL14-immunoreactive cells were detected in the peripheral region of the pancreatic islets and were immunoreactive for somatostatin, but not for glucagon, insulin, and pancreatic polypeptide. Immunoelectron microscopy indicated that the CXCL14-like peptide and somatostatin co-existed in the secretory granules. CXCL14, secreted from somatostatin-containing cells, may modulate insulin secretion in a paracrine fashion, and play a novel role in glucose homeostasis in addition to its well-known chemotactic activities...
December 25, 2015: Acta Histochemica et Cytochemica
Toshihiro Hosokawa, Yoshiki Tsuchiya, Naoki Okubo, Tatsuya Kunimoto, Yoichi Minami, Hiroyoshi Fujiwara, Yasuhiro Umemura, Nobuya Koike, Toshikazu Kubo, Kazuhiro Yagita
Cartilage tissues possess intrinsic circadian oscillators, which influence chondrocyte function and chondrocyte specific gene expression. However, it is not fully understood how chondrogenesis influences the circadian clock, and vice versa. Thus, we established ATDC5 cells which were stably transfected with the Bmal1:luc reporter and revealed robust circadian rhythms in ATDC5 cells during differentiation. Moreover, the circadian clock in ATDC5 cells was strongly reset by PTH in a circadian time-dependent manner...
December 25, 2015: Acta Histochemica et Cytochemica
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