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Clinical Biochemistry

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https://www.readbyqxmd.com/read/29782819/analytical-performance-of-an-immunoassay-to-measure-proenkephalin
#1
Leslie J Donato, Jeffrey W Meeusen, John C Lieske, Deborah Bergmann, Andrea Sparwaßer, Allan S Jaffe
BACKGROUND: Endogenous opioids, enkephalins, are known to increase with acute kidney injury. Since the mature pentapeptides are unstable, we evaluated the performance of an assay that measures proenkephalin 119-159 (PENK), a stable peptide formed concomitantly with mature enkephalins. METHODS: PENK assay performance was evaluated on two microtiterplate/chemiluminescence sandwich immunoassay formats that required 18 or 1 h incubation times. PENK concentration was measured in plasma from healthy individuals to establish a reference interval and in patients with varied levels of kidney function and comorbidities to assess the association with measured glomerular filtration rate (mGFR) using iothalamate clearance...
May 18, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29777658/case-report-uncommon-sulfamethoxazole-crystalluria
#2
Vincent Castiglione, Etienne Cavalier, Romy Gadisseur
No abstract text is available yet for this article.
May 16, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29763574/how-low-can-you-go-analytical-performance-of-five-automated-testosterone-immunoassays
#3
Sonia L La'ulu, Kimberly J Kalp, Joely A Straseski
BACKGROUND: Testosterone is commonly measured using immunoassays, yet concerns with the accuracy and quality of testing by these methods exist, particularly for low testosterone concentrations. Study objectives were to evaluate selective performance characteristics, including functional sensitivity (FS), of 5 automated immunoassays for total testosterone. METHODS: FS, imprecision, assay interference, limit of blank, linearity, and accuracy were assessed using the Abbott ARCHITECT i2000SR , SIEMENS ADVIA Centaur and IMMULITE 2000, Beckman Coulter DxI 800, and Roche MODULAR E170...
May 12, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29750940/the-importance-of-low-level-qc-for-high-sensitivity-troponin-assays
#4
Peter E Hickman, Gus Koerbin, Tony Badrick, Carmen Oakman, Julia M Potter
BACKGROUND: With the advent of the new high-sensitivity troponin assays, it is becoming critical to measure troponin accurately to low concentrations. To ensure assay performance is acceptable, appropriate QC must be run. METHODS: In addition to the routine use of commercial QC material, we prepared pools of human QC material with low troponin concentrations close to the limit of quantitation, and ran these regularly on our laboratory analysers. RESULTS: Over 3 years we found no drift or shift in our hs-cTnI assay...
May 8, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29750939/first-high-sensitivity-cardiac-troponin-i-assay-cleared-by-the-united-states-food-and-drug-administration-validation-and-implications
#5
Robert H Christenson, Kristin Mullins, Show-Hong Duh
BACKGROUND: High-Sensitivity (hs) cardiac troponin (cTn) assays are categorized by two criteria: (i) cTn values above the limit of detection (LoD) for >50% of male and female healthy cohorts of ≥300 individuals; (ii) imprecision ≤10% total %CV for sex-specific 99th-percentile clinical decision values (CDVs). No documented hs-Tn assay has yet been FDA-cleared. METHODS: The PATHFAST cTnI-II assay's LoD was 2.3 ng/L using CLSI EP-17. The AACC Universal Sample Bank of 847 healthy men (50...
May 8, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29750938/different-top-down-approaches-to-estimate-measurement-uncertainty-of-whole-blood-tacrolimus-mass-concentration-values
#6
Raül Rigo-Bonnin, Aurora Blanco-Font, Francesca Canalias
BACKGROUND: Values of mass concentration of tacrolimus in whole blood are commonly used by the clinicians for monitoring the status of a transplant patient and for checking whether the administered dose of tacrolimus is effective. So, clinical laboratories must provide results as accurately as possible. Measurement uncertainty can allow ensuring reliability of these results. The aim of this study was to estimate measurement uncertainty of whole blood mass concentration tacrolimus values obtained by UHPLC-MS/MS using two top-down approaches: the single laboratory validation approach and the proficiency testing approach...
May 8, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29750937/the-potential-role-of-a-turbidimetric-heart-type-fatty-acid-binding-protein-assay-to-aid-in-the-interpretation-of-persistently-elevated-non-changing-cardiac-troponin-i-concentrations
#7
Peter A Kavsak, Craig Ainsworth, Donald M Arnold, Terry Scott, Lorna Clark, Josko Ivica, Katharine Mackett, Richard Whitlock, Andrew Worster
BACKGROUND: Elevated and non-changing high-sensitivity cardiac troponin (hs-cTn) concentrations may suggest a process other than acute injury, possibly due to chronic condition(s) causing the elevation, an analytical error/interference or the formation of macrocomplexes. Heart-type fatty acid binding protein (H-FABP) might be useful in this setting to identify the etiology of abnormally high and non-changing cTn concentrations which could aid clinical decision making in the hospital setting...
May 8, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29746826/high-sensitivity-cardiac-troponin-i-and-risk-of-incident-atrial-fibrillation-hospitalisation-in-an-australian-community-based-cohort-the-busselton-health-study
#8
Kun Zhu, Joseph Hung, Mark Divitini, Kevin Murray, Ee Mun Lim, Andrew St John, John P Walsh, Matthew Knuiman
OBJECTIVE: The identification of individuals at risk of atrial fibrillation (AF) remains a challenge. We investigated whether high-sensitive cardiac troponin I (hs-cTnI) is an independent predictor of incident atrial fibrillation (AF) hospitalisation in an Australian community-based cohort. METHODS: Serum hs-cTnI was measured in 1641 men and 2189 women in the Busselton Health Study 1994/1995 Cohort aged 25-84 years at baseline. Data on incident AF hospitalisation over 20 years follow-up were obtained by data linkage...
May 7, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29738695/an-approach-to-analytical-validation-and-testing-of-body-fluid-assays-for-the-automated-clinical-laboratory
#9
Darci R Block, Lucas J Ouverson, Craig A Wittwer, Amy K Saenger, Nikola A Baumann
BACKGROUND: Biochemical analysis of body fluids may lend insight into the pathogenesis of disease. Most commercially-available clinical laboratory methods are intended for blood-derived specimens and/or urine and laboratories must characterize the analytical performance of these methods for other specimen types such as body fluids. The aim of this work is to demonstrate one approach for characterizing analytical performance of assays for clinical analysis of body fluids. METHODS: Residual waste samples were obtained from clinically ordered testing...
May 5, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29729229/triple-test-with-tumor-markers-cyfra-21-1-he4-and-progrp-might-contribute-to-diagnosis-and-subtyping-of-lung-cancer
#10
Elif Tugce Korkmaz, Deniz Koksal, Funda Aksu, Z Gunnur Dikmen, Duygu Icen, Emin Maden, Sevgen Onder, Filiz Akbiyik, Salih Emri
BACKGROUND AND AIM: Early diagnosis and histological subtyping are important issues in the management of patients with lung cancer (LC). The aim of this study is to investigate the diagnostic value of a panel of serum tumor markers in newly diagnosed patients with LC. METHODS: Venous blood samples were collected from 99 patients with LC (42 adenocarcinoma, 35 squamous, and 22 small cell carcinoma) and 30 patients with benign lung disease. Progastrin releasing peptide (ProGRP), squamous cell carcinoma antigen (SCCAg), cytokeratin 19-fragments (CYFRA 21...
May 2, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29709501/spurious-testosterone-laboratory-results-in-a-patient-taking-synthetic-alkaline-phosphatase-asfotase-alfa
#11
Alina G Sofronescu, Meredith Ross, Eric Rush, Whitney Goldner
OBJECTIVES: We report a case of discordant total and free testosterone values in a patient with hypogonadism and juvenile hypophosphatasia after he initiated treatment with asfotase alfa, recombinant tissue non-specific alkaline phosphatase. METHODS: Total testosterone was evaluated using immunoassay pre and post initiation of therapy with asfotase alfa, and free testosterone was evaluated using radioimmunoassay and LC-MS/MS while on asfotase alfa therapy. RESULTS: Total testosterone measured by immunoassay was normal prior to therapy with asfotase alfa, and was low post initiation of therapy...
April 27, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29702062/in-response-to-letter-to-editor-by-vivek-kumar-garg-et-al-on-our-paper-dysregulation-of-serum-nadph-oxidase1-and-ferritin-levels-provides-insights-into-diagnosis-of-parkinson-s-disease-hemmati-dinarvand-m-et-al-2017-50-18-1087-1092
#12
LETTER
Mohsen Hemmati-Dinarvand, Aliakbar Taheraghdam, Ali Mota, Sepideh Zununi Vahed, Nasser Samadi
No abstract text is available yet for this article.
April 24, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29702061/aggrecanase-degradation-of-type-iii-collagen-is-associated-with-clinical-knee-pain
#13
A C Bay-Jensen, C F Kjelgaard-Petersen, K K Petersen, L Arendt-Nielsen, H L Quasnichka, A Mobasheri, M A Karsdal, D J Leeming
There is a lack of biochemical markers for non-invasive and objective assessment of symptomatic osteoarthritis (OA). Aggrecanase activity has been shown to be associated with joint deterioration and symptomatic disease through the degradation of extracellular matrix proteins, such as type III collagen. Our study aimed to identify and develop a novel biomarker by measuring an aggrecanase-mediated type III collagen neoepitope, and correlate levels of this biomarker with OA joint pain. Mass spectrometric analysis of purified type III collagen, degraded by the aggrecanase A Disintigrin and Metalloproteinase with Thrombospondin motif (ADAMTS), revealed a fragment generated by ADAMTS-1, -4 and -8...
April 24, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29698621/diagnostic-and-prognostic-value-of-h-fabp-in-acute-coronary-syndrome-still-evidence-to-bring
#14
REVIEW
Giulia Bivona, Luisa Agnello, Chiara Bellia, Bruna Lo Sasso, Marcello Ciaccio
The assessment of chest pain patients presenting to the emergency area (EA) is still a clinical challenge, as the majority of patients are not diagnosed with ACS. New generation high sensitivity c-Tn (hs-cTn) assays have showed better performances compared to the standard c-Tn. However, hs-Tn still presents some limitations. Hence, novel, early biomarkers are needed in this setting. Among all, heart-type fatty acid binding protein (H-FABP) has been largely investigated. This article reviews the studies evaluating H-FABP performance in diagnosing AMI and stratifying chest pain patients by risk...
April 23, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29684370/analytical-validation-and-investigation-on-reference-intervals-of-aldosterone-and-renin-in-chinese-han-population-by-using-fully-automated-chemiluminescence-immunoassays
#15
Lingyan Deng, Zhigang Xiong, Huijun Li, Xiaomei Lei, Liming Cheng
BACKGROUND: The aldosterone/renin ratio (ARR) is recommended to screen for primary aldosteronism (PA) in hypertension. We estimated fully automated chemiluminescence immunoassays (CLIA) for plasma aldosterone concentrations (PAC) and plasma direct renin concentrations (PRC) and investigated their reference intervals in Chinese Han population. METHODS: PAC and PRC were measured on a fully automated analyzer (LIAISON XL, DiaSorin, Italy). Performance characteristics were estimated according to CLSI proved guidelines...
April 20, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29684369/alpha-fetoprotein-in-pericardial-peritoneal-and-pleural-fluids-a-body-fluid-matrix-evaluation
#16
William E Owen, Joshua J H Hunsaker, Jonathan R Genzen
OBJECTIVES: Alpha-fetoprotein (AFP) measurement in pericardial, peritoneal (ascites), and pleural fluids is sometimes requested by clinicians as supportive evidence in the evaluation of suspected malignancy. As commercially available, Food and Drug Administration (FDA)-cleared AFP assays are not validated for these fluid types, laboratories must complete additional validation studies to comply with regulatory requirements for body fluid testing. The objective of this study was therefore to conduct a matrix evaluation for these body fluid types using the Beckman Access AFP assay on the UniCel DxI 800 immunoassay system...
April 20, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29684368/order-of-draw-practices-in-venous-blood-sampling-at-clinical-biochemistry-departments-in-the-danish-health-care-system
#17
Katja Kemp Jacobsen, Ida Brandt, Anne Vindahl Christensen, Bjørk Anine Rimsø, Camilla Julie Krøier, Michelle Sørensen, Julie Smith, Kathrine Overgaard Foss Jensen, Jeppe Madura Larsen
BACKGROUND: Deviation in blood collection procedures is a central source of preanalytical variation affecting overall analytical and diagnostic precision. The order of draw of venous sampling is suspected to affect analytical results, in particular for coagulation analysis. Here we compare the procedures in venous blood sampling among clinical biochemistry departments to assess the uniformity of order of blood draw and adherence to international guidelines in the Danish health care system...
April 20, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29684367/commutability-and-traceability-in-eqa-programs
#18
Tony Badrick, Wilson Punyalack, Peter Graham
OBJECTIVES: The concept of commutability of samples has focused laboratories on the importance of traceability. However, the critical role of External Quality Assurance (EQA) in achieving the primary role of traceability (i.e. facilitating comparable patient results in different laboratories) has largely been lost. The aim of this paper is to review the role of EQA in achieving traceable results. DESIGN AND METHODS: The role of commutability and traceability in EQA and Internal Quality Control (IQC) are discussed...
April 20, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29680706/elevated-igm-against-n%C3%AE%C2%B5-carboxyethyl-lysine-modified-apolipoprotein-a1-peptide-141-147-in-taiwanese-with-alzheimer-s-disease
#19
Ching-Yu Lin, Jau-Jiuan Sheu, I-Shih Tsai, Sen-Te Wang, Li-Yu Yang, I-Uen Hsu, Hui-Wen Chang, Horng-Mo Lee, Shu-Huei Kao, Ching-Kuo Lee, Chien-Ho Chen, Yung-Feng Lin
OBJECTIVE: Advanced glycation end products (AGEs) are involved in the pathogenesis of Alzheimer's disease (AD). Specific AGEs and related autoantibodies may be early AD markers. Apolipoprotein A1 (ApoA1) and its post-translational modifications (PTMs) are associated with neurodegeneration and thus selected to test the hypothesis. METHODS: Serum samples from totally 64 AD or health control (HC) Taiwanese were analyzed. ApoA1 was isolated from the serum and examined through LC-MS/MS and PTM analyses...
April 19, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29679552/serum-retinol-binding-protein-4-and-galectin-3-binding-protein-as-novel-markers-for-postmenopausal-nonalcoholic-fatty-liver-disease
#20
Hong Cai, Sha Lu, Yueming Chen, Sayantana Das Mbbs Mrcog, Zheng Niu, Guangchao Zhuo, Lei Lai, Zhifen Zhang
OBJECTIVE: To investigate the differential protein expression before and after menopause in women with nonalcoholic fatty liver disease (NAFLD) and to explore novel markers for menopausal NAFLD. METHODS: Eight serum samples collected from pre- or post-menopausal women with NAFLD were analysed by iTRAQ 2D-LC-MS/MS. Two protein candidates were selected and verified by enzyme-linked immunosorbent assay (ELISA) in serum samples collected from a one hundred and fifty-three female population subsequently, including 51 in post-menopausal status with NAFLD, 41 in pre-menopausal with NAFLD, 19 healthy individuals in post-menopausal status and 42 healthy pre-menopausal women...
April 19, 2018: Clinical Biochemistry
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