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Clinical Biochemistry

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https://www.readbyqxmd.com/read/28941592/dysregulation-of-serum-nadph-oxidase1-and-ferritin-levels-provides-insights-into-diagnosis-of-parkinson-s-disease
#1
Mohsen Hemmati-Dinarvand, Ali-Akbar Taher-Aghdam, Ali Mota, Sepideh Zununi Vahed, Nasser Samadi
OBJECTIVE: Parkinson's disease (PD) is a common neurodegenerative disease. Oxidative stress is considered as a key modulator in the development of PD. This study aimed to investigate associations between serum NOX1 (NADPH oxidase1), ferritin, selenium (Se) and uric acid (UA) levels and clinical parameters in patients with PD. DESIGN AND METHODS: Serum levels of NOX1, ferritin, Se and UA were measured in 40 PD patients and 40 healthy individuals. Receiver operating characteristic (ROC) analysis was performed to investigate incremental diagnostic value of each factor in the study groups...
September 20, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28941591/ige-monoclonal-gammopathy-a-case-report-and-literature-review
#2
REVIEW
C Hejl, R Mestiri, T Carmoi, S Bugier, D Chianea, C Renard, P Vest
Immunoglobulin E (IgE) gammopathy is a rare disorder, accounting for just 0.1% of all patients with multiple myeloma (MM). Herein, we report a case of IgE monoclonal gammopathy without any biological and clinical symptoms, and we review 63 published cases in the literature. Demographic, biological and clinical presentations and features appear to be similar to those of other subtypes of MM, with a median age of diagnosis of 67years. There is a slight excess of male patients, and incidence seems to increase with age...
September 20, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28941590/method-performance-and-clinical-workflow-outcomes-associated-with-meconium-and-umbilical-cord-toxicology-testing
#3
Ruth M Labardee, Jaime R Swartzwelder, Karen E Gebhardt, Justine A Pardi, Anna C Dawsey, R Brent Dixon, Steven W Cotten
OBJECTIVE: Neonatal abstinence syndrome (NAS) is a rising concern with unknown long-term effects. It is apparent that higher cost of care, impact on the community and reduced quality of life are associated with similar etiologies (e.g., fetal alcohol syndrome). Detection of drug exposure in utero allows for earlier intervention to potentially reduce undesired outcomes. Umbilical cord tissue (UCT) has been documented as a readily accessible specimen for detection of drug exposure and has emerged as an alternative specimen to meconium...
September 20, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28928007/removal-of-uremic-retention-products-by-hemodialysis-is-coupled-with-indiscriminate-loss-of-vital-metabolites
#4
Zhi-Hao Zhang, Jia-Rong Mao, Hua Chen, Wei Su, Yuan Zhang, Li Zhang, Dan-Qian Chen, Ying-Yong Zhao, Nosratola D Vaziri
BACKGROUND: Although dialysis ameliorates uremia and fluid and electrolytes disorders, annual mortality rate remains high in dialysis population reflecting its shortcoming in replacing renal function. Unlike the normal kidney, dialysis causes dramatic shifts in volume and composition of body fluids and indiscriminate removal of vital solutes. Present study was undertaken to determine the impact of hemodialysis on plasma metabolites in end-stage renal disease (ESRD) patients. METHODS: 80 hemodialysis patients and 80 age/gender-matched healthy controls were enrolled in the study...
September 18, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28928006/combining-nordtest-method-and-bootstrap-resampling-for-measurement-uncertainty-estimation-of-hematology-analytes-in-a-medical-laboratory
#5
Ming Cui, Lili Xu, Huimin Wang, Shaoqing Ju, Shuizhu Xu, Rongrong Jing
BACKGROUND: Measurement uncertainty (MU) is a metrological concept, which can be used for objectively estimating the quality of test results in medical laboratories. The Nordtest guide recommends an approach that uses both internal quality control (IQC) and external quality assessment (EQA) data to evaluate the MU. Bootstrap resampling is employed to simulate the unknown distribution based on the mathematical statistics method using an existing small sample of data, where the aim is to transform the small sample into a large sample...
September 18, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28928005/sensitive-and-selective-quantification-of-total-and-free-itraconazole-and-hydroxyitraconazole-in-human-plasma-using-ultra-performance-liquid-chromatography-coupled-to-tandem-mass-spectrometry
#6
Yosuke Suzuki, Ryota Tanaka, Nobuhiro Oyama, Ko Nonoshita, Kazuhiko Hashinaga, Kenji Umeki, Yuhki Sato, Kazufumi Hiramatsu, Jun-Ichi Kadota, Hiroki Itoh
OBJECTIVES: Protein-free (unbound) drug concentrations have been reported to be better biomarker of pharmacodynamics compared with total drug concentrations. In this study, we developed and validated an assay for the quantification of total and free itraconazole and hydroxyitraconazole, a main metabolite with antifungal activity, in human plasma using ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). DESIGN & METHODS: Plasma sample was ultra-filtrated for the measurement of free itraconazole and hydroxyitraconazole concentrations...
September 16, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28918132/assessing-precision-bias-and-sigma-metrics-of-53-measurands-of-the-alinity-ci-system
#7
Sten Westgard, Victoria Petrides, Sharon Schneider, Marvin Berman, Jörg Herzogenrath, Anthony Orzechowski
Assay performance is dependent on the accuracy and precision of a given method. These attributes can be combined into an analytical Sigma-metric, providing a simple value for laboratorians to use in evaluating a test method's capability to meet its analytical quality requirements. Sigma-metrics were determined for 37 clinical chemistry assays, 13 immunoassays, and 3 ICT methods on the Alinity ci system. METHODS: Analytical Performance Specifications were defined for the assays, following a rationale of using CLIA goals first, then Ricos Desirable goals when CLIA did not regular the method, and then other sources if the Ricos Desirable goal was unrealistic...
September 13, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28916437/sex-specific-99th-percentiles-derived-from-the-aacc-universal-sample-bank-for-the-roche-gen-5-ctnt-assay-comorbidities-and-statistical-methods-influence-derivation-of-reference-limits
#8
Ian L Gunsolus, Allan S Jaffe, Anne Sexter, Karen Schulz, Ranka Ler, Brittany Lindgren, Amy K Saenger, Sara A Love, Fred S Apple
OBJECTIVES: Our purpose was to determine a) overall and sex-specific 99th percentile upper reference limits (URL) and b) influences of statistical methods and comorbidities on the URLs. METHODS: Heparin plasma from 838 normal subjects (423 men, 415 women) were obtained from the AACC (Universal Sample Bank). The cobas e602 measured cTnT (Roche Gen 5 assay); limit of detection (LoD), 3ng/L. Hemoglobin A1c (URL 6.5%), NT-proBNP (URL 125ng/L) and eGFR (60mL/min/1.73m(2)) were measured, along with identification of statin use, to better define normality...
September 13, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28916439/on-the-path-to-evidence-based-reporting-of-serum-protein-electrophoresis-patterns-in-the-absence-of-a-discernible-monoclonal-protein-a-critical-review-of-literature-and-practice-suggestions
#9
Pak Cheung Chan, Yu Chen, Edward W Randell
No abstract text is available yet for this article.
September 12, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28916438/analytical-sensitivity-and-diagnostic-performance-of-serum-protein-electrophoresis-on-the-hydragel-30-protein-e-%C3%AE-1-%C3%AE-2-sebia-hydrasys-system
#10
Albert K Y Tsui, Dylan Thomas, Alison Hunt, Mathew Estey, Cathy-Lou Christensen, Trefor Higgins, Irwindeep Sandhu, Karina Rodriguez-Capote
BACKGROUND: Serum protein electrophoresis (SPE) and immunofixation electrophoresis (IFE) are used in the diagnosis and monitoring of plasma cell dyscrasias. IFE is considered the most sensitive method for the detection of monoclonal proteins (M-proteins), but it is not quantitative. The goal of this study was to establish the analytical sensitivity and diagnostic performance of SPE on the Sebia Hydrasys using HYDRAGEL 30 PROTEIN(E) β1-β2. METHODOLOGY: Patient sera with a previously identified M-protein (IgG, IgA or IgM) were serially diluted with a normal serum pool and electrophoresed on the Sebia Hydrasys using HYDRAGEL 30 PROTEIN(E) β1-β2...
September 12, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28912097/igg-subclasses-quantitation-analytical-performance-of-the-binding-site-spaplus%C3%A2-human-assay-and-comparison-with-siemens-bnii%C3%A2-assay
#11
Ana Sarnago, Rosa M Pascual, María J Moreno, Begoña Laíz, Oscar Fuster
OBJECTIVES: Accurate evaluation of analyzers is highly recommended before these devices are broadly introduced for routine testing. Concerning quantification of IgG subclasses (IgGSc), standardization has not yet been reached and thus different assays might lead to different results. Here we report the analytical performances of The Binding Site (TBS) SPAPLUS® human IgGSc assay and the concordance with the Siemens BNII® human IgGSc assay. DESIGN AND METHODS: We evaluated precision, LoB, LoD and linearity of TBS SPAPLUS® human IgGSc immunoassay...
September 11, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28912096/analytical-verification-and-method-comparison-of-the-advia-centaur%C3%A2-intact-parathyroid-hormone-assay
#12
Esther Fernández, Josep Lluís Bedini, Xavier Filella
OBJECTIVES: This study is the first verification of the novel iPTH Siemens ADVIA Centaur® Intact Parathyroid Hormone (iPTHm) chemiluminescence immunoassay based on monoclonal antibodies. We also compared the iPTH results obtained using this assay with the previous ADVIA Centaur® Parathyroid Hormone assay (iPTHp) based on polyclonal antibodies. DESIGN AND METHODS: The analytical performance study of iPTHm assay included LoD, LoQ, intra- and inter-assay reproducibility, and linearity...
September 11, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28899631/biochemical-differences-in-the-mass-and-activity-tests-of-lipoprotein-associated-phospholipase-a2-explain-the-discordance-in-results-between-the-two-assay-methods
#13
Shaoqiu Zhuo, Robert L Wolfert, Chong Yuan
OBJECTIVES: There are two platforms for the detection of Lp-PLA2 in sera or plasmas: by its enzymatic activity (PLAC® activity test) and by its mass concentration (PLAC® mass test). It has been long recognized that these two platforms are not correlated well. The underlying cause for this is therefore investigated by the biochemical characterization of the two PLAC tests. DESIGN & METHODS: Human sera with and without the treatment by detergent were fractionated by using a Superose-6 column in phosphate buffered saline and the phospholipid associated Lp-PLA2 was assessed by both PLAC mass and activity tests...
September 9, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28893597/evaluation-of-a-provincial-intervention-to-reduce-redundant-hemoglobin-a1c-testing
#14
Irene Ma, Maggie Guo, Jeannine Viczko, Christopher Naugler
BACKGROUND: The Canadian Diabetes Association recommends testing most individuals with diabetes mellitus using HbA1c once every 3months. In Alberta, Canada, a provincial intervention to reduce inappropriate HbA1c test orders was implemented to align with this guideline, where only one HbA1c test request every 90days for most individuals can be processed. Here, we evaluated the provincial intervention to reduce redundant HbA1c testing in Alberta. METHODS: Total counts of all HbA1c tests ordered within Edmonton and Calgary, Alberta, were collected from the respective Laboratory Information Systems each month between January 2013 and December 2016...
September 8, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28888879/fast-form-alpha-2-macroglobulin-a-marker-for-protease-activation-in-plasma-exposed-to-artificial-surfaces
#15
Daniel Biltoft, Jørgen B Gram, Anette Larsen, Anna-Marie B Münster, Johannes J Sidelmann, Karsten Skjoedt, Yaseelan Palarasah
OBJECTIVES: Investigation of the blood compatibility requires a number of sensitive assays to quantify the activation of the blood protein cascades and cells induced by biomaterials. A global assay measuring the blood compatibility of biomaterials could be a valuable tool in such regard. In this study, we investigated whether an enzyme-linked immunosorbent assay (ELISA), that specifically measures the electrophoretic "fast form" of α2-macroglobulin (F-α2M), could be a sensitive and global marker for activation of calcium dependent and in-dependent proteases in plasma exposed to biomaterials in vitro...
September 6, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28888878/effect-of-vitamin-d-supplementation-on-knee-osteoarthritis-a-systematic-review-and-meta-analysis-of-randomized-clinical-trials
#16
REVIEW
Naicheng Diao, Bo Yang, Fei Yu
OBJECTIVE: To provide evidence regarding the effect of vitamin D supplementation on symptomatic knee osteoarthritis (OA). METHODS: A systematic review and meta-analysis was performed to quantitatively pool the results from randomized clinical trials. Studies were identified from a search of the Embase, MEDLINE and Web of Science databases up to January 22, 2017, and also from conference abstracts, ClinicalTrials.gov and the reference lists of identified studies...
September 6, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28867331/reference-ranges-for-serum-s100b-neuroprotein-specific-to-infants-under-four-months-of-age
#17
Jeanne Simon-Pimmel, Fleur Lorton, Damien Masson, Damien Bouvier, Matthieu Hanf, Christèle Gras-Le Guen
BACKGROUND: Minor head traumatisms are a common reason for consultation in paediatric emergency departments. The diagnosis of traumatic brain injuries involves performing a cranial computed tomography (CCT), associated with a risk of cancer due to the radiation. The serum S100B is an effective biomarker used to reduce reliance on CCT. While reference ranges have been determined, the limited number of cases regarding infants less than 4months of age has not allowed this biomarker to be used with this age group...
September 1, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28864294/preanalytical-errors-in-transfusion-medicine-reply-to-74-year-old-female-with-new-monoclonal-protein-on-serum-immunofixation-electrophoresis
#18
LETTER
Caleb Cheng, Amit Gokhale, Christopher A Tormey, Jeanne E Hendrickson, Burak Bahar
No abstract text is available yet for this article.
August 30, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28860054/detection-of-contactin-2-in-cerebrospinal-fluid-csf-of-patients-with-alzheimer-s-disease-using-fluorescence-correlation-spectroscopy-fcs
#19
Madhurima Chatterjee, Bernd Nöding, Eline A J Willemse, Marleen J A Koel-Simmelink, Wiesje M van der Flier, Detlev Schild, Charlotte E Teunissen
OBJECTIVES: Alzheimer's disease (AD) is the most common cause of dementia in the world. As many AD biomarkers occur at rather low abundances in CSF or blood, techniques of very high sensitivity and accuracy are important as diagnostic tools in the clinic. Here, we aimed to provide proof of concept of the use of a single molecule detection technique, Fluorescence Correlation Spectroscopy (FCS) for detection of novel candidate biomarkers for AD. DESIGN AND METHODS: FCS detects the diffusion times of the antigen-antibody complexes in highly diluted sample solutions, thus eliminating the need of large sample volumes and allows estimating the concentration of the target antigen...
August 28, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28847718/the-investigation-of-interferences-in-immunoassay
#20
REVIEW
Greg Ward, Aaron Simpson, Lyn Boscato, Peter E Hickman
Immunoassay procedures have a wide application in clinical medicine and as such are used throughout clinical biochemistry laboratories both for urgent and routine testing. Clinicians and laboratory personnel are often presented with immunoassay results which are inconsistent with clinical findings. Without a high index of suspicion interferences will often not be suspected. Artifactual results can be due to a range of interferences in immunoassays which can include cross reacting substances, heterophile antibodies, autoantibodies and the high dose hook effect...
August 26, 2017: Clinical Biochemistry
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