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Clinical Biochemistry

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https://www.readbyqxmd.com/read/28803963/model-analysis-of-bidirectional-interference-in-two-stage-labeled-ligand-immunoassays
#1
Eleanor R Lewin, Amitava Dasgupta, Roland Valdes, Douglas F Stickle
OBJECTIVES: Immunoassays involving sample incubation followed by a wash step prior to introduction of labeled analyte are potentially subject to both positive and negative interference (bidirectional interference) by a competing ligand. We examine this phenomenon from a theoretical standpoint using a mathematical model for sequential-step immunoassays in the presence of interferent. DESIGN & METHODS: Competitive binding to antibody between analyte and interferent was modeled for sequential-step immunoassays...
August 10, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28803962/post-analytical-stability-of-23-common-chemistry-and-immunochemistry-analytes-in-incurred-samples
#2
Betina Klint Nielsen, Tina Frederiksen, Lennart Friis-Hansen, Pia Bükmann Larsen
BACKGROUND: Storage of blood samples after centrifugation, decapping and initial sampling allows ordering of additional blood tests. The pre-analytic stability of biochemistry and immunochemistry analytes has been studied in detail, but little is known about the post-analytical stability in incurred samples. METHODS: We examined the stability of 23 routine analytes on the Dimension Vista® (Siemens Healthineers, Denmark): 42-60 routine samples in lithium-heparin gel tubes (Vacutainer, BD, USA) were centrifuged at 3000×g for 10min...
August 10, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28803961/the-99th-percentile-and-imprecision-of-point-of-care-cardiac-troponin-i-in-comparison-to-central-laboratory-tests-in-a-large-reference-population
#3
Anne Greiser, Theresa Winter, Hala Mahfoud, Anders Kallner, Till Ittermann, Annette Masuch, Norbert Lubenow, Thomas Kohlmann, Andreas Greinacher, Matthias Nauck, Astrid Petersmann
OBJECTIVES: Determination of cardiac troponin (cTn) is central in the emergency department (ED) for the diagnosis of myocardial infarction. In view of adverse effects of long waiting time on patient outcome, implementation of point-of-care-testing (POCT) is suggested if the turn-around-time is longer than 60min. The present study aimed to determine the 99th percentile and imprecision of two POCT in a healthy population measuring cTnI and cTnT and compare these analytical characteristics against three central laboratory test (CLT) for cTnI...
August 10, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28802863/comparison-of-three-different-assays-for-measuring-thyroglobulin-and-thyroglobulin-antibodies-in-patients-with-chronic-lymphocytic-thyroiditis
#4
Xinqi Cheng, Songlin Yu, Cheng Jin, Song Han, Yingying Hu, Kui Zhang, Huaicheng Liu, Ling Qiu
OBJECTIVE: To evaluate how thyroglobulin (Tg) and thyroglobulin antibody (TgAb) results compare among different chemiluminescence methods. METHODS: Serum specimens from 153 patients with thyroiditis and 127 apparently healthy individuals were collected at the Peking Union Medical College Hospital from September 2014 to December 2014. TgAb and Tg levels were analyzed by using three different platforms manufactured by Roche, Beckman, and Siemens. The χ(2) test was performed to analyze the TgAb results from the different methods...
August 9, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28789859/performance-characteristics-of-a-high-sensitivity-cardiac-troponin-assay-using-plasma-and-whole-blood-samples
#5
P Li, N S Enea, R Zuk, H Tan, A H B Wu, A S Jaffe
No abstract text is available yet for this article.
August 5, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28782522/a-critical-appraisal-of-a-methodology-for-estimating-analytical-variation-from-sequential-patient-blood-gas-measurements
#6
LETTER
Sohrab Mansouri, Paul D'Orazio, Sharon Erhmeyer
No abstract text is available yet for this article.
August 4, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28779954/paraoxonase-1-pon1-and-stroke-the-dilemma-of-the-genetic-variation
#7
REVIEW
Amir Tajbakhsh, Mehdi Rezaee, Mahdi Rivandi, Faranak Forouzanfar, Fahimeh Afzaljavan, Alireza Pasdar
Ischaemic stroke, which is mostly caused by atherosclerosis, is one of the most widely causes of mortality and morbidity. One of the most important enzymatic activities of paraoxonase-1 (PON1) in association with high-density lipoprotein (HDL) is the prevention of low-density lipoprotein (LDL) oxidation, which gives it an anti-atherogenic activity. PON1 expression and activity is influenced by various factors; the most important of which is genetic polymorphism, mainly single nucleotide polymorphisms (SNPs)...
August 2, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28764886/are-the-thiol-disulfide-redox-status-and-hdl-cholesterol-levels-associated-with-pulmonary-embolism-thiol-disulfide-redox-status-in-pulmonary-embolism
#8
Ebru Sengul Parlak, Murat Alisik, Aysegul Karalezli, Ayse Gokcen Sayilir, Serdal Bastug, Mukremin Er, Hatice Canan Hasanoglu, Ozcan Erel
No abstract text is available yet for this article.
July 29, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28764885/plasma-oxalate-in-relation-to-egfr-in-patients-with-primary-hyperoxaluria-enteric-hyperoxaluria-and-urinary-stone-disease
#9
Majuran Perinpam, Felicity T Enders, Kristin C Mara, Lisa E Vaughan, Ramila A Mehta, Nickolay Voskoboev, Dawn S Milliner, John C Lieske
BACKGROUND: Since plasma oxalate (POx) concentrations increase at lower glomerular filtration rate (GFR) levels, even among those without enteric (EH) or primary hyperoxaluria (PH), the appropriate thresholds for considering a disorder of oxalate metabolism are poorly defined. The current study was completed to establish relationships between POx, GFR, and urine oxalate excretion (UOx) among patients with PH, EH, and routine urinary stone disease (USD). METHODS: The most recent POx measurement on all Mayo Clinic patients between 2005 and 2015 were electronically pulled from the Lab Information System together with the closest serum creatinine within 14days and 24h urine study within 60days...
July 29, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28756070/diagnostic-and-prognostic-value-of-cystatin-c-in-acute-heart-failure
#10
Tobias Breidthardt, Zaid Sabti, Ronny Ziller, Frank Rassouli, Raphael Twerenbold, Nikola Kozhuharov, Etienne Gayat, Samyut Shrestha, Sara Barata, Patrick Badertscher, Jasper Boeddinghaus, Thomas Nestelberger, Christian Mueller
BACKGROUND: The accurate early diagnosis of acute kidney injury (AKI) in patients with acute heart failure (AHF) is an unmet clinical need. Cystatin C might improve the early detection of AKI. METHODS: 207 patients presenting to the emergency department with AHF were enrolled. Cystatin C was measured in plasma in a blinded fashion at presentation and serially thereafter. The potential of Cystatin C levels to predict AKI was assessed as the primary endpoint. Long-term mortality was assessed as a secondary endpoint...
July 26, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28754333/effect-of-allopurinol-and-uric-acid-normalization-on-serum-lipids-hyperuricemic-subjects-a-systematic-review-with-meta-analysis
#11
REVIEW
Verônica Moreira Ferreira de Castro, Angelita Cristine de Melo, Vinícius Silva Belo, Valéria Ernestânia Chaves
Although uric acid is not part of any definition of metabolic syndrome, a number of studies have shown strong associations between the concentration of uric acid and metabolic syndrome or its components. The purpose of this systematic review with meta-analysis was to evaluate, using prospective interventional studies, the effects of allopurinol therapy and uric acid normalization on serum concentrations of triacylglycerol, total-cholesterol, LDL-cholesterol and HDL-cholesterol in hyperuricemic subjects. A systematic search of the PubMed and Scopus databases was performed following the guidelines described in the PRISMA statement...
July 25, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28754332/mortality-risk-of-elevated-alkaline-phosphatase-in-patients-with-coronary-artery-disease-and-percutaneous-coronary-intervention
#12
LETTER
Tomoyuki Kawada
No abstract text is available yet for this article.
July 25, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28754331/response-to-the-letter-to-the-editor-mortality-risk-of-elevated-alkaline-phosphatase-in-patients-with-coronary-artery-disease-and-percutaneous-coronary-intervention
#13
LETTER
Gjin Ndrepepa, Adnan Kastrati
No abstract text is available yet for this article.
July 25, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28746862/generation-of-monoclonal-antibodies-and-development-of-an-immunofluorometric-assay-for-the-detection-of-cuzd1-in-tissues-and-biological-fluids
#14
Sofia Farkona, Antoninus Soosaipillai, Panagiota Filippou, Dimitrios Korbakis, Stefano Serra, Felix Rückert, Eleftherios P Diamandis, Ivan M Blasutig
BACKGROUND: CUB and zona pellucida-like domain-containing protein 1 (CUZD1) was identified as a pancreas-specific protein and was proposed as a candidate biomarker for pancreatic related disorders. CUZD1 protein levels in tissues and biological fluids have not been extensively examined. The purpose of the present study was to generate specific antibodies targeting CUZD1 to assess CUZD1 expression within tissues and biological fluids. METHODS: Mouse monoclonal antibodies against CUZD1 were generated and used to perform immunohistochemical analyses and to develop a sensitive and specific enzyme-linked immunosorbent assay (ELISA)...
July 23, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28739222/biomarkers-of-cancer-cachexia
#15
REVIEW
Audrey Loumaye, Jean-Paul Thissen
Cachexia is a complex multifactorial syndrome, characterized by loss of skeletal muscle and fat mass, which affects the majority of advanced cancer patients and is associated with poor prognosis. Interestingly, reversing muscle loss in animal models of cancer cachexia leads to prolong survival. Therefore, detecting cachexia and maintaining muscle mass represent a major goal in the care of cancer patients. However, early diagnosis of cancer cachexia is currently limited for several reasons. Indeed, cachexia development is variable according to tumor and host characteristics...
July 21, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28738179/erratum-to-establishing-reference-intervals-for-galectin-3-concentrations-in-serum-requires-careful-consideration-of-its-biological-determinants-clin-biochem-50-2017-599-604
#16
Magdalena Krintus, Marek Kozinski, Tomasz Fabiszak, Jacek Kubica, Mauro Panteghini, Grazyna Sypniewska
No abstract text is available yet for this article.
July 21, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28736056/variability-of-glycated-hemoglobin-levels-in-the-first-year-post-renal-transplantation-in-patients-without-diabetes
#17
Ana Laura Pimentel, Joíza Lins Camargo
BACKGROUND: Glycated hemoglobin (HbA1c) is an alternative test used for the diagnosis and monitoring of diabetes in kidney transplant recipients. Immunosuppressive drugs are the most important risk factors related with changes in the glucose metabolism after transplant. It is still unknown if they impact on the variability of HbA1c. We assessed the variability of HbA1c levels in a group of renal transplant recipients without diabetes during the first year after transplant. METHODS: We estimated the variability of HbA1c in a group of 95 Brazilian kidney transplant recipients...
July 20, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28736055/monitoring-urinary-orosomucoid-in-patients-undergoing-cardiac-surgery-a-promising-novel-inflammatory-marker
#18
Péter Kustán, Balázs Szirmay, Tamás Kőszegi, Andrea Ludány, Gábor L Kovács, Attila Miseta, Diána Mühl, Balázs Németh, István Kiss, Ádám Németh, Sándor Szabados, Zénó Ajtay
BACKGROUND: Urinary biomarkers might provide non-invasive tool for monitoring of systemic processes. We aimed to investigate the time-course of urinary orosomucoid (u-ORM) excretion after cardiac surgery hypothesizing that u-ORM is an early and sensitive marker of systemic inflammatory activation. METHODS: During a 5-day follow-up study we monitored u-ORM levels in cardiovascular patients who underwent on-pump cardiac surgery (n=38). The patients baseline data were compared to healthy control individuals (n=40)...
July 20, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28736054/low-sensitivity-of-anion-gap-to-detect-clinically-significant-lactic-acidosis-in-the-emergency-department
#19
Q Xu, S HowlettClyne, A Fuezery, G S Cembrowski
INTRODUCTION: Lactic acidosis represents the pathologic accumulation of lactate and hydrogen ions. It is important to efficiently diagnose lactic acidosis as delayed treatment will lead to poor patient outcomes. As plasma lactate levels may not be rapidly available, some physicians may use elevated anion gaps to test for the need to measure lactate. All Edmonton metropolitan hospitals have Radiometer blood gas/electrolyte instruments in the ED or close by. As lactate is measured for each set of electrolytes, we were able to determine the effectiveness of a screening anion gap for lactic acidosis...
July 20, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28734906/results-from-15years-of-quality-surveillance-for-a-national-indigenous-point-of-care-testing-program-for-diabetes
#20
Mark Shephard, Anne Shephard, Bridgit McAteer, Tamika Regnier, Kristina Barancek
INTRODUCTION: Diabetes is a major health problem for Australia's Aboriginal and Torres Strait Islander peoples. Point-of-care testing for haemoglobin A1c (HbA1c) has been the cornerstone of a long-standing program (QAAMS) to manage glycaemic control in Indigenous people with diabetes and recently, to diagnose diabetes. METHODS: The QAAMS quality management framework includes monthly testing of quality control (QC) and external quality assurance (EQA) samples. Key performance indicators of quality include imprecision (coefficient of variation [CV%]) and percentage acceptable results...
July 19, 2017: Clinical Biochemistry
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