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Clinical Biochemistry

Michael Oellerich, Philipp Kanzow, Philip D Walson
No abstract text is available yet for this article.
January 14, 2017: Clinical Biochemistry
Rebecca K McCann, Karen Smith, Daniel R Gaya
BACKGROUND: Whilst C-reactive protein (CRP) is an established serum marker of inflammation, its use in gastroenterology has been limited by its poor sensitivity and specificity for GI disease.Faecal calprotectin (FC) has been adopted into mainstream GI practice as a sensitive but non-specific marker of intestinal inflammation. However, stool samples collection for FC can be challenging and the possibility of utilising a sensitive and specific serum biomarker of intestinal inflammation in luminal gastroenterology is an attractive prospect...
January 13, 2017: Clinical Biochemistry
Ana Carolina Breier, Jaqueline Cé, Jamila Mezzalira, Vanessa V Daitx, Vitoria C Moraes, Mariana P S Goldim, Janice C Coelho
BACKGROUND: The goal of this study was to assess the biochemical parameters of the enzymes α-l-iduronidase (IDUA) and arylsulfatase B (ASB), which are deficient in mucopolysaccharidosis (MPS) I and VI, respectively, in dried blood spot (DBS) samples impregnated on filter paper. METHODS AND RESULTS: The optimal pH, Km, and Vmax of IDUA and ASB in DBS are hereby presented. After these analyses, the reference values for the activities of these enzymes in DBS with cutoff of 3...
January 11, 2017: Clinical Biochemistry
Ola Z Ismail, Vipin Bhayana, Michael Kadour, Nathalie Lepage, Manjula Gowrishankar, Guido Filler
No abstract text is available yet for this article.
January 11, 2017: Clinical Biochemistry
Christopher McCudden
No abstract text is available yet for this article.
January 10, 2017: Clinical Biochemistry
Simon A Mahler, Jason P Stopyra, Fred S Apple, Robert F Riley, Gregory B Russell, Brian C Hiestand, James W Hoekstra, Cedric W Lefebvre, Bret A Nicks, David M Cline, Kim L Askew, David M Herrington, Gregory L Burke, Chadwick D Miller
OBJECTIVES: The HEART Pathway combines a decision aid and serial contemporary cardiac troponin I (cTnI) measures to achieve >99% sensitivity for major adverse cardiac events (MACE) at 30days and early discharge rates >20%. However, the impact of integrating high-sensitivity troponin (hs-cTn) measures into the HEART Pathway has yet to be determined. In this analysis we compare test characteristics of the HEART Pathway using hs-cTnI, hs-cTnT, or cTnI. DESIGN & METHODS: A secondary analysis of participants enrolled in the HEART Pathway RCT was conducted...
January 10, 2017: Clinical Biochemistry
Brittany N Goldstein, Jordan Wesler, Amy S Nowacki, Edmunds Reineks, Marvin R Natowicz
An assessment of blood ammonia concentration is common medical practice in the evaluation of an individual with an unexplained mental status change or coma. The determination of a blood ammonia level is most commonly done using a glutamate dehydrogenase (GLDH)-based assay, although there are many potential sources of artifact and the literature is inconsistent regarding key preanalytic issues. Using a GLDH-based assay, we first investigated matrix effects using three anticoagulants: heparin, EDTA and oxalate...
January 7, 2017: Clinical Biochemistry
C Bobin-Dubigeon, A Lefrançois, D Vansteene, M Dupé, M-P Joalland, Jean-Marie Bard
OBJECTIVES: To evaluate leptin and adiponectin as markers of undernutrition in cancer patients, and compared their performances with those of other biomarkers. DESIGN AND METHODS: This was a prospective and observational study of 132 patients with various types of cancer. Following the recommended professional criteria, we diagnosed undernutrition at the time of blood sampling for the biological analysis of leptin, adiponectin, paraoxonase (hydrolysis rate of three substrates: paraoxon (PON), phenylacetate (ARE) and thiolactone (LAC)), and the calculation of the Prognostic Inflammatory and Nutritional Index (PINI)...
January 6, 2017: Clinical Biochemistry
Philip Goldwasser, Andrea Roche-Recinos, Robert H Barth
OBJECTIVES: Sodium concentration is measured by either indirect (INa) or direct potentiometry (DNa), on chemistry and gas panels, respectively. A spurious difference between these methods (ΔNa=INa-DNa) can be confusing to the clinician. For example, variation in serum total protein (TP) is well known to selectively interfere with INa. Red cells have been suggested to interfere with DNa, but both positive and negative interference have been reported. In this study, the effect of gas panel hemoglobin (Hb) on ΔNa was examined...
January 5, 2017: Clinical Biochemistry
Mohammad Osman Pervez, Magnus Nakrem Lyngbakken, Peder Langeland Myhre, Jon Brynildsen, Eva Camilla Langsjøen, Arne Didrik Høiseth, Geir Christensen, Torbjørn Omland, Helge Røsjø
BACKGROUND: Mid-regional pro-adrenomedullin (MR-proADM) is a surrogate marker for adrenomedullin; a hormone that attenuates myocardial remodeling. Accordingly, we hypothesized that MR-proADM could provide diagnostic and prognostic information in patients with acute dyspnea. METHODS AND RESULTS: We measured MR-proADM by a commercial ELISA on hospital admission in 311 patients with acute dyspnea and compared the utility of MR-proADM with N-terminal pro-B-type natriuretic peptide (NT-proBNP)...
January 5, 2017: Clinical Biochemistry
Lars Komorowski, Winfried Stoecker, Johanna Fraune, Christian Probst
No abstract text is available yet for this article.
December 30, 2016: Clinical Biochemistry
Thomas Boehm, Sophie Pils, Elisabeth Gludovacz, Helen Szoelloesi, Karin Petroczi, Otto Majdic, Andrea Quaroni, Nicole Borth, Peter Valent, Bernd Jilma
OBJECTIVES: Diamine oxidase (DAO) is essential for extracellular degradation of histamine. For decades activity assays with inherent limitations were used to quantify the relative amounts of DAO. No reference DAO standard is available. Absolute DAO amounts cannot be determined. Controversy exists, whether DAO is circulating or not in non-pregnant individuals. The role of DAO as biomarker in various diseases is ambiguous. It is not clear, whether precise quantification of human DAO antigen using commercially available enzyme-linked immunosorbent assays (ELISAs) is possible...
December 29, 2016: Clinical Biochemistry
Camilla Linder, Katarina Wide, Malin Walander, Olof Beck, Lars L Gustafsson, Anton Pohanka
OBJECTIVES: To investigate if dried blood spots could be used for therapeutic drug monitoring of the antiepileptic drugs, carbamazepine, lamotrigine and valproic acid in children with epilepsy. METHODS: Fingerprick blood samples from 46 children at a neuropediatric outpatient clinic was collected on filterpaper at the same time as capillary plasma sampling. A validated dried blood spot liquid chromatography tandem mass spectrometry method for carbamazepine, lamotrigine and valproic acid was compared with the routine plasma laboratory methods...
December 24, 2016: Clinical Biochemistry
Florence Van Obbergh, Laurent Knoops, Timothy Devos, Yves Beguin, Carlos Graux, Fleur Benghiat, Khalil Kargar-Samani, Deborah Bauwens, André Efira, Christian Dubois, Cécile Springael, Luc Montfort, Thierry Connerotte, Arnaud Capron, André Delannoy, Pierre Wallemacq
: This retrospective multicenter study in patients with chronic myeloid leukemia in chronic phase was undertaken to confirm the clinical relevance of imatinib plasma concentrations monitoring in daily practice. Forty-one patients, with 47 imatinib plasma measurements, were analyzed during treatment with imatinib given at a fixed 400mg daily dose. A significant inverse relationship of imatinib concentration with the patients' weight was observed (Pearson's test: p=0.02, R(2)=0.1). More interestingly, patients with poor response (switched to another tyrosine kinase inhibitor because of imatinib failure, or because of disease progression after an initial response) displayed a significantly lower mean imatinib concentration as compared to patients maintained on imatinib (822ng/mL vs 1099ng/mL; Student's t-test, p=0...
December 22, 2016: Clinical Biochemistry
Guilherme A Behr, Jay P Patel, Marg Coote, Jose C F Moreira, Daniel P Gelain, Meir Steiner, Benicio N Frey
OBJECTIVES: Previous studies have reported that salivary concentrations of certain hormones correlate with their respective serum levels. However, most of these studies did not control for potential blood contamination in saliva. In the present study we developed a statistical method to test the amount of blood contamination that needs to be avoided in saliva samples for the following hormones: cortisol, estradiol, progesterone, testosterone and oxytocin. DESIGN & METHODS: Saliva and serum samples were collected from 38 healthy, medication-free women (mean age=33...
December 16, 2016: Clinical Biochemistry
Pia Bükmann Larsen, Allan Linneberg, Torben Hansen, Lennart Friis-Hansen
BACKGROUND: Despite international efforts to standardize C-peptide and insulin calibrators and immunoassays, platform dependent differences still exist, and platform specific reference intervals are hence needed for correct interpretation. We therefore wanted to establish traceable reference intervals for C-peptide and insulin. METHODS: In 623 consecutively recruited participants, insulin and C-peptide were measured using the Cobas e411 (Roche Diagnostics, Switzerland)...
December 16, 2016: Clinical Biochemistry
Steven Hy Wong, Kamisha L Johnson-Davis, Krista Garrison, Joe D Rankin, Chro S Muhammad
OBJECTIVES: Everolimus (EVR), a mTOR inhibitor immunosuppressant approved for renal, liver and cardiac transplants. This study established the appropriate TDM performances of the Thermo Scientific QMS EVR Assay by using the Beckman DxC, followed by comparison to the Thermo Scientific Indiko and a previously published LC-MS/MS assay, and Beckman AU680 and AU5800 analyzers. DESIGN AND METHODS: The study initially established acceptable linearity, precision and accuracy of the QMS EVR turbidimetric assay...
December 15, 2016: Clinical Biochemistry
Asma Blel, Yann Orven, Nicolas Pallet, Jean François Chasse, Benoit Vedie, Marie Anne Loriot, Jean Louis Paul, Céline Narjoz
No abstract text is available yet for this article.
December 14, 2016: Clinical Biochemistry
Dimitrios Kerimis, Christos K Kontos, Spyridon Christodoulou, Iordanis N Papadopoulos, Andreas Scorilas
OBJECTIVES: Several miRNAs are aberrantly expressed in cancer. miR-24-3p is involved in cancer-related cellular processes, including cell cycle control, cell growth, proliferation, and apoptosis. In this study, we examined the potential diagnostic and prognostic significance of miR-24-3p expression in colorectal adenocarcinoma. DESIGN AND METHODS: Total RNA was isolated from 182 colorectal adenocarcinoma specimens and 86 paired non-cancerous colorectal mucosae. After polyadenylation of 2μg total RNA and reverse transcription into first-strand cDNA using an oligo-dT-adapter primer, miR-24-3p expression was quantified using an in-house-developed reverse-transcription real-time quantitative PCR method, based on the SYBR Green chemistry...
December 7, 2016: Clinical Biochemistry
Jinny Jeffery, Zoe J Vincent, Ruth M Ayling, Stephen J Lewis
BACKGROUND: Metronidazole is an oral antibiotic which is widely used in the treatment of patients with Clostridium difficile associated disease. METHODS: This article describes the validation of a LC-MS/MS assay for the measurement of metronidazole in human faecal samples. RESULTS: Matrix matched and aqueous standards showed no significant difference in performance for the routine calibration of the assay. D(4) deuterated metronidazole internal standard eluted with a different retention time to the undeuterated metronidazole on chromatography, hence zidovudine was used as an internal standard...
December 5, 2016: Clinical Biochemistry
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