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Clinical Biochemistry

D M Donegan, A Algeciras-Schimnich, O Hamidi, W F Young, T Nippoldt, I Bancos, D Erickson
Measuring the plasma corticotropin (ACTH) concentration is an important step in determining the underlying cause in patients with hypercortisolemia. Interfering substances in immunoassays can lead to erroneous results impacting clinical management. We describe a case series of 12 patients, the majority of whom were being investigated for possible Cushing's syndrome and in whom inconsistencies between the clinical picture and biochemical testing raised concerns of assay interference. ACTH assay interference resulted in falsely elevated ACTH concentrations using the Siemens Immulite assay and consequently led to additional unnecessary testing...
November 10, 2018: Clinical Biochemistry
Ying Zhang, Weibo Ma, Guocheng Wang, Yaqi Lv, Yaguang Peng, Xiaoxia Peng
OBJECTIVE: To examine the effect of different limits of acceptability on the Hoffmann method for selecting "healthy" populations from laboratory test data. METHODS: Thyroid stimulating hormone (TSH) and free thyroxine (FT4 ) were measured in Shunyi Women's and Children's Hospital of Beijing Children's Hospital. The sample size of participants of TSH and FT4 for reference intervals (RIs) establishment was 10,864 and 10,799, respectively. RIs were calculated by Hoffmann method with different acceptable deviations (α value)...
November 8, 2018: Clinical Biochemistry
Saif Zaman, Boris I Chobrutskiy, Jay S Patel, Blake M Callahan, Wei Lue Tong, Moody M Mihyu, George Blanck
OBJECTIVE: To assess the potential impact of mutant ECM amino acids (AA) on melanoma-related matrix metalloproteinase-7 (MMP7) activity. DESIGN AND METHODS: We applied a novel scripted algorithm, based on the MEROPS database, to reveal mutant-dependent sensitivity changes across the cancer genome atlas, melanoma dataset. RESULTS: This approach revealed a strong bias in favor of mutant AA dependent protease sensitivity increases. Thus, melanoma specimens with relatively few mutations had only MMP7 mutant sensitive, ECM peptides...
November 8, 2018: Clinical Biochemistry
Yutong Zhang, Rui Ban, Qiang Shi, Chenglin Tian
BACKGROUND: The measurement of carcinoembryonic antigen, carbohydrate antigen series biomarkers in cerebrospinal fluid (CSF), is useful for the diagnosis of brain metastasis and leptomeningeal metastases to a certain extent. Their serum/CSF ratios may be of benefit to earlier diagnosis and treatment. However, the normal reference values of the ratios were not available. Accordingly, in this study we analyzed the serum/CSF ratios of tumor markers levels in non-neoplastic diseases patients for possible normal values...
November 4, 2018: Clinical Biochemistry
Gregory Baxter-Parker, Lloyd Roffe, Sean Cross, Chris Frampton, Gary J Hooper, Steven Gieseg
CONTEXT: Knee arthroplasty surgery is significant trauma, leading to an activated immune system causing inflammation and oxidative stress. Many current biomarkers are invasive, costly, and often slow to analyse, limiting their use for rapid inflammatory measurements. OBJECTIVES: We have examined the use of urinary neopterin and total neopterin in knee arthroplasty patients to non-invasively measure oxidative stress and inflammation from immune system activation...
November 3, 2018: Clinical Biochemistry
Vasilije Novakovic, Samire Abdija, Trine Borowik, Lene Gredal, Kirsten Vikkelsø Madsen, Katja Kemp Jacobsen
BACKGROUND: Deviation in blood collection procedures is a central source of preanalytical variation affecting overall analytical and diagnostic precision. The procedure of venous blood collection for ionized calcium is hypothesized to affect analytical results. Here, we evaluate the effect of blood collection with and without a discard tube, and storage duration on results of P-Ionized Calcium (pH adjusted = 7.4). METHODS: We collected 100 paired venous blood tubes from randomly selected outpatients using a winged blood collection...
November 2, 2018: Clinical Biochemistry
Saranya Kittanakom, Vinh Ly, Anthony Arnoldo, Aimee Beattie, Peter A Kavsak
No abstract text is available yet for this article.
October 27, 2018: Clinical Biochemistry
Damien Gruson
Oxytocin (OT) is a nonapeptide hormone mainly synthesized in the magnocellular neurons of the paraventricular and supraoptic nucleus of the hypothalamus. In the extra-hypothalamic brain areas, OT acts like neurotransmitters and modulators. The physiological functions of OT are multiple. OT participates to the coordination and control of gonadal development and reproduction. OT appears also as an important regulator of social behaviors such as affiliative, parental, and romantic behaviors. Recent evidence suggests other roles for OT such as potent effects on cardiometabolic functions or involvement in stress-related disorders...
October 27, 2018: Clinical Biochemistry
Jose Cervera, Sohrab Mansouri, Prasad V A Pamidi
No abstract text is available yet for this article.
October 24, 2018: Clinical Biochemistry
Michael K Palmer, Philip J Barter, Pia Lundman, Stephen J Nicholls, Peter P Toth, Björn W Karlson
Treating elevated low-density lipoprotein cholesterol (LDL-C) to risk-stratified target levels is recommended in several guidelines. Thus, accurate estimation of LDL-C is required. LDL-C is typically calculated using the Friedewald equation: (total cholesterol) - (non-high-density lipoprotein cholesterol [non-HDL-C]) - (triglycerides/5). As the equation uses a fixed value equal to 5 as a divisor for triglycerides, it does not account for inter-individual variability, often resulting in underestimation of risk and potentially undertreatment...
October 23, 2018: Clinical Biochemistry
Christine Wyns, Joely Straseski, Damien Gruson
No abstract text is available yet for this article.
October 19, 2018: Clinical Biochemistry
Megumi Sato, Ryunosuke Ohkawa, Hann Low, Madoka Nishimori, Shigeo Okubo, Akira Yoshimoto, Kouji Yano, Takahiro Kameda, Yutaka Yatomi, Minoru Tozuka
BACKGROUND: Serum amyloid A (SAA), which is one of the acute phase proteins, alters the structure of HDL by associating with it during circulation. We focused on whether SAA influences the values of HDL-cholesterol (HDL-C) measurements when using a homogeneous assay. METHODS: HDLs were isolated by ultracentrifugation from serum samples of 248 patients that were stratified into three groups based on their serum SAA concentrations (low: SAA ≤ 8 μg/mL; middle: 8 < SAA ≤ 100 μg/mL; and high: SAA > 100 μg/mL)...
October 17, 2018: Clinical Biochemistry
Jérôme A Denis, Alessandra Mazzola, Guillaume Nguyen, Jean-Marc Lacorte, Christine Brochet, Annette K Larsen, Filoména Conti
CA 19-9 (carbohydrate antigen 19-9) is a tumor marker widely used for surveillance of patients with pancreatic cancer. However, even high levels of CA 19-9 may not necessarily be cancer-associated thereby complicating the diagnosis. This case report highlights a transient increase of CA 19-9 in a triple transplanted patient with cystic fibrosis and continuous immunosuppression for 20 years who was under antibiotics. This case emphasizes the need for a balanced interpretation of biological results, especially in cases where many confounding factors are present such as diabetes, chronic renal failure, cystic fibrosis and infections...
October 17, 2018: Clinical Biochemistry
Susana Coimbra, Flávio Reis, Cátia Ferreira, Sara Nunes, Sofia Viana, Alice Catarino, Petronila Rocha-Pereira, Luís Belo, Luís Monteiro, Cristina Catarino, Alice Santos-Silva
OBJECTIVES: Weight loss achieved by laparoscopic adjustable gastric banding (LAGB) induces an increase in high-density lipoprotein cholesterol (HDLc) but a small effect on low-density lipoprotein (LDL), although changes in their quality (size and composition) are uncertain. Our aim was to study the impact of weight loss, achieved 13-months after LAGB, on inflammation and dyslipidemia, focusing on HDL and LDL subfractions, and oxidized LDL (oxLDL). DESIGN & METHODS: We evaluated standard lipid profile, HDL and LDL subfractions, oxLDL, interleukin (IL)-6 and C-reactive protein (CRP), in twenty obese patients, before (T0) and 13-months after LAGB (T1), and in seventeen healthy controls...
October 17, 2018: Clinical Biochemistry
Lucie Petrova, Filip Vrbacky, Miriam Lanska, Alzbeta Zavrelova, Pavel Zak, Katerina Hrochova
No abstract text is available yet for this article.
October 16, 2018: Clinical Biochemistry
Paul D Simonson, Katie H Kim, Gabrielle Winston-McPherson, Rugvedita S Parakh, Diane Yamaguchi, Anna E Merrill, Jane A Dickerson, Dina N Greene
BACKGROUND: Due to the narrow therapeutic range of digoxin, determining serum/plasma digoxin concentrations is critical for assessing patients with congestive heart failure, atrial fibrillation, and certain types of arrhythmias. However, digoxin quantification by competitive immunoassays is susceptible to interferences that may alter the accuracy of its measurement in patient plasma. This study aimed to characterize the extent of bilirubin interference in three commonly used digoxin immunoassays...
October 11, 2018: Clinical Biochemistry
Angelo Minucci, Maurizio Lalle, Rossella De Leo, Giorgia Mazzuccato, Giovanni Scambia, Andrea Urbani, Anna Fagotti, Paola Concolino, Ettore Capoluongo
OBJECTIVES: In silico splicing analysis, a mini-gene assay and splicing data, obtained using RNA from blood samples, have shown that the BRCA1 c.5332G > A variant induces exon 21 skipping. However, despite these evidences, up to date, this variant is unclassified. The aim of this study is to provide further molecular and clinical evidence for the BRCA1 c.5332G > A variant in a patient with high grade serous ovarian carcinoma (HGSOC) to allow a definitive classification of this variant...
October 10, 2018: Clinical Biochemistry
Jennifer Taher, Jake Cosme, Brian A Renley, David J Daghfal, Paul M Yip
OBJECTIVES: The Abbott Alinity family of chemistry and immunoassay systems recently launched with early adopters contributing imprecision and bias data, which was consolidated to assess the performance of Alinity assays across multiple sites using the Sigma metric. Multi-site Sigma metrics were determined for 3 ion-selective electrodes, 12 photometric assays, and 3 immunoassays across 11 independent laboratory sites in 9 countries. METHODS: Total allowable error (TEa) goals followed a previously defined hierarchy that used CLIA as the primary goal...
October 10, 2018: Clinical Biochemistry
Jianqiu Wu, Weiyan Tang, Laiquan Huang, Ning Hou, Jing Wu, Xianfeng Cheng, Dawei Ma, Pudong Qian, Qian Shen, Wenjie Guo, Wei Peng, Yufei Liu, Cunshun Jiang, Jifeng Feng
OBJECTIVE: To evaluate cell-free DNA (cfDNA) in plasma as a promising biomarker for lymphoma, altered levels of cfDNA and its association with clinical parameters are investigated in patients suffered from lymphomas. METHODS: Peripheral blood specimens were collected from 60 patients with lymphoma during initial diagnosis and those of another 107 patients with lymphoma during treated stage were also collected, 93 healthy volunteers were selected as control group...
October 4, 2018: Clinical Biochemistry
Raül Rigo-Bonnin, Francesca Canalias
BACKGROUND: Due to the high inter-variability in immunosuppressants pharmacokinetics, therapeutic drug monitoring of these drugs is essential in order to minimize the risk of rejection after organ transplantation. Thus, results facilitated to clinicians by clinical laboratories for these drugs should be as reliable as possible. The knowledge of metrological traceability and performing compatibility studies can allow ensuring reliability of these results, mainly, when laboratories introduce substantial changes in their measurement procedures, e...
October 3, 2018: Clinical Biochemistry
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