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Clinical Biochemistry

Zufferey Fanny, Rahban Rita, Garcia Arnaud, Gagnebin Yoric, Boccard Julien, Tonoli David, Jeanneret Fabienne, Stettler Eric, Senn Alfred, Nef Serge, Rudaz Serge, F Rossier Michel
Steroids play an important role in sperm production and quality. These hormones have been extensively studied in blood, but poorly investigated in semen. The purpose of our study was to evaluate the relationship between sperm quality and steroid profiles in blood and semen in a small cohort of young Swiss men. Another objective was to determine whether the presence of xenobiotics or drugs could influence these profiles. Semen analysis was performed according to WHO guidelines, and steroid profiles in blood serum and seminal plasma were determined by two complementary approaches: a targeted investigation involving the quantification of a limited number of relevant steroids for testing putative correlations with sperm parameters and a global "steroidomic" analysis highlighting their complex metabolic relationship...
March 16, 2018: Clinical Biochemistry
Vander Borght Mélodie, Wyns Christine
Infertility is a disease characterized by the failure to establish a clinical pregnancy after 12 months of regular and unprotected sexual intercourse. It is estimated to affect between 8 and 12% of reproductive-aged couples worldwide. Males are found to be solely responsible for 20-30% of infertility cases but contribute to 50% of cases overall. Secondary infertility is the most common form of female infertility around the globe, often due to reproductive tract infections. The three major factors influencing the spontaneous probability of conception are the time of unwanted non-conception, the age of the female partner and the disease-related infertility...
March 16, 2018: Clinical Biochemistry
Jose C Jara-Aguirre, Steven W Smeets, Amy M Wockenfus, Brad S Karon
OBJECTIVE: Evaluate the effects of blood gas sample contamination with total parenteral nutrition (TPN)/lipid emulsion and dextrose 50% (D50) solutions on blood gas and electrolyte measurement; and determine whether glucose concentration can predict blood gas sample contamination with TPN/lipid emulsion or D50. DESIGN AND METHODS: Residual lithium heparin arterial blood gas samples were spiked with TPN/lipid emulsion (0 to 15%) and D50 solutions (0 to 2.5%). Blood gas (pH, pCO2, pO2), electrolytes (Na+, K+ ionized calcium) and hemoglobin were measured with a Radiometer ABL90...
March 16, 2018: Clinical Biochemistry
Tiago Antonio Tonietto, Marcio Manozzo Boniatti, Thiago Costa Lisboa, Marina Verçoza Viana, Moreno Calcagnotto Dos Santos, Carla Silva Lincho, José Augusto Santos Pellegrini, Josi Vidart, Jeruza Lavanholi Neyeloff, Gustavo Adolpho Moreira Faulhaber
BACKGROUND: Red blood cell distribution width (RDW) is a predictor of mortality in critically ill patients. Our objective was to investigate the association between the RDW at ICU discharge and the risk of ICU readmission or unexpected death in the ward. METHODS: A secondary analysis of prospectively collected data study was conducted including patients discharged alive from the ICU to the ward. The target variable was the RDW collected at ICU discharge. Elevated RDW was defined as an RDW > 16%...
March 14, 2018: Clinical Biochemistry
Andrea Padoan, Laura Sciacovelli, Ada Aita, Giorgia Antonelli, Mario Plebani
BACKGROUND: Measurement uncertainty (MU) estimation has been introduced by ISO 15189 for the accreditation of clinical laboratories. Although MU reporting is not required, its inclusion in medical reports is of potential assistance to physicians in results interpretation. METHODS: MU reporting was evaluated with respect to different test purposes, namely comparison with reference intervals (RI), patient monitoring or comparison with clinical decision limits. Clinical Biochemistry, Hematology, Coagulation and Clinical Immunology measurands were used as examples...
March 13, 2018: Clinical Biochemistry
Alexey A Konev, Daria V Serebryanaya, Ekaterina V Koshkina, Fedor V Rozov, Vladimir L Filatov, Stanislav V Kozlovsky, Andrey N Kara, Alexey G Katrukha, Alexander B Postnikov
BACKGROUND: N-terminal and C-terminal proteolytic fragments of IGF binding protein 4 (NT-IGFBP-4 and CT-IGFBP-4) were recently shown to predict adverse cardiac events in acute coronary syndrome (ACS) patients. NT-IGFBP-4 and CT-IGFBP-4 are products of the pregnancy-associated plasma protein-A (PAPP-A)-mediated cleavage of IGFBP-4. It has been demonstrated that circulating IGFBP-4 is partially glycosylated in its N-terminal region, although the influence of this glycosylation on PAPP-A-mediated proteolysis and the ratio of glycosylated/non-glycosylated IGFBP-4 fragments in human blood remain unrevealed...
March 8, 2018: Clinical Biochemistry
Federica Braga, Mauro Panteghini
In addition to the correct implementation of calibration traceability, the definition and the achievement of an appropriate analytical performance specification for the total uncertainty budget (GU ) is essential to ensure that laboratory measurements are clinically usable. To understand if it is possible to fulfil these specifications, limits for combined uncertainty across the entire metrological traceability chain should be defined. We recommended that no more than one third of GU should be consumed by the uncertainty of higher order references and ≤50% of GU by the combined measurement uncertainty at the manufacturer's calibration level...
March 8, 2018: Clinical Biochemistry
Carel J Pretorius, Jillian R Tate, Urs Wilgen, Louise Cullen, Jacobus P J Ungerer
INTRODUCTION: We investigated the analytical performance, outlier rate, carryover and reference interval of the Beckman Coulter Access hsTnI in detail and compared it with historical and other commercial assays. MATERIALS AND METHODS: We compared the imprecision, detection capability, analytical sensitivity, outlier rate and carryover against two previous Access AccuTnI assay versions. We established the reference interval with stored samples from a previous study and compared the concordances and variances with the Access AccuTnI+3 as well as with two commercial assays...
March 7, 2018: Clinical Biochemistry
Teddy Léguillier, Romain Jouffroy, Marie Boisson, Agathe Boussaroque, Camille Chenevier-Gobeaux, Tarek Chaabouni, Benoît Vivien, Valérie Nivet-Antoine, Jean-Louis Beaudeux
AIM OF THE STUDY: We evaluated if the StatStrip Xpress Meter, a Lactate point of care testing (POCT) handled device, could be a valuable tool in the mobile intensive care units (MICU) to assess the severity of septic patients. METHODS: We first investigated POCT analytical performance, then, using samples collected from 50 identified septic patients admitted to the intensive care unit (ICU), we compared lactate values obtained with the device to those obtained with four central laboratory analysers: one whole blood and three plasma-based methods...
March 6, 2018: Clinical Biochemistry
Edward W Randell, Garry Short, Natasha Lee, Allison Beresford, Margaret Spencer, Marina Kennel, Zoë Moores, David Parry
OBJECTIVE: This study examines effectiveness of a project to enhance an autoverification (AV) system through application of Six Sigma (DMAIC) process improvement strategies. DESIGN AND METHODS: Similar AV systems set up at three sites underwent examination and modification to produce improved systems while monitoring proportions of samples autoverified, the time required for manual review and verification, sample processing time, and examining characteristics of tests not autoverified...
March 5, 2018: Clinical Biochemistry
Dong Ying, Congming Hong, Guiping Wen, Zimin Tang, Siling Wang, Xu Zhang, Chang Liu, Shengxiang Ge, Zizheng Zheng, Ningshao Xia
BACKGROUND: Hepatitis E virus (HEV)-caused acute viral hepatitis is a major threat to public health worldwide. Recently, an enzyme linked immunosorbent assay (ELISA) kit detecting the HEV antigen was reported to have good concordance with the HEV RNA load and showed good clinical performance. But the ELISA kits can barely satisfy the needs of community clinics. In this study, a fluorescent microbead-based immunoassay (FMIA) for detecting the HEV antigen was developed and evaluated. METHODS: A mouse anti-HEV monoclonal antibody (mAb) conjugated with fluorescent microbeads was used as capturing antibody and another mouse mAb was used as detection antibody...
March 5, 2018: Clinical Biochemistry
Hugo Alberto Rojas, Karla Simone da Silva Fernandes, Mariana Rodrigues Ottone, Kênia Cristina S Fonseca de Magalhães, Lucas Alverne Albuquerque, Julio Leonardo Barbosa Pereira, Gerival Vieira Júnior, José Lopes Sousa-Filho, Bruno Silva Costa, Valéria Cristina Sandrim, Marcos Dellaretti, Renata Toscano Simões
BACKGROUND: Intracranial aneurysms are arterial anomalies affecting 2% to 3% of the general population in the world and these ruptures are associated with a high mortality. Some risk factors, such as age, gender, smoking, alcohol, hypertension and familial history are associated with the number of aneurysms and their size. In addition, inflammatory processes within the blood vessels of the brain can activate matrix metalloproteinase-9 (MMP-9), which degrades various components of the extracellular matrix, such as elastin...
March 5, 2018: Clinical Biochemistry
Natália B Andriguetti, Roberta Z Hahn, Lilian F Lizot, Suziane Raymundo, Jose L Costa, Kelly F da Cunha, Ramon M M Vilela, Helena M Kluck, Gilberto Schwartsmann, Marina V Antunes, Rafael Linden
BACKGROUND: Paclitaxel (PCT) is a chemotherapeutic drug widely used for the treatment of several types of tumors, and its use is associated with severe adverse events, mainly neurologic and hematopoietic toxicities. The relation between systemic exposure and clinical response to PCT was previously described, making paclitaxel a potential candidate for therapeutic drug monitoring (TDM). The use of dried blood spot (DBS) sampling could allow complex sampling schedules required for TDM of PCT...
March 4, 2018: Clinical Biochemistry
Giuseppe Lippi, Janne Cadamuro, Alexander von Meyer, Ana-Maria Simundic
No abstract text is available yet for this article.
March 3, 2018: Clinical Biochemistry
Clare Fiala, Eleftherios P Diamandis
No abstract text is available yet for this article.
March 2, 2018: Clinical Biochemistry
Etienne Cavalier, Margot Salsé, Anne-Marie Dupuy, Anne-Sophie Bargnoux, Florence Watar, Jean-Claude Souberbielle, Pierre Delanaye, Jean-Paul Cristol
BACKGROUND: 3rd generation PTH assays only detect the bioactive 1-84 fragment. Since standardization is still lacking, each new PTH assay requires to establish reference values and to assess the impact in the medical care of the mineral and bone disorders in hemodialyzed patients. METHODS: Using Fujirebio Lumipulse G wPTH assay, serum PTH levels were measured in a population of 439 healthy subjects from France and Belgium PTH levels were also determined in 119 hemodialyzed patients...
February 28, 2018: Clinical Biochemistry
Manal Elnenaei, Derek Minney, David B Clarke, Andrew Kumar-Misir, Syed Ali Imran
OBJECTIVE: The clinical presentation of pituitary dysfunction is typically variable and may often be insidious, resulting in delayed diagnosis by up to decades. The complexity of presentation and difficulty in pattern recognition of first line hormone tests result in challenges in early diagnosis of this condition. The aim of this study was to determine the impact of reflective testing and interpretive commenting on the early detection and management of such cases from primary care. METHODS: Prospective audit over 12 months in which first line pituitary target organ hormones were identified via a reflex algorithm in the laboratory information system...
February 24, 2018: Clinical Biochemistry
Chiara Bellia, Martina Zaninotto, Chiara Cosma, Luisa Agnello, Giulia Bivona, Mariela Marinova, Bruna Lo Sasso, Mario Plebani, Marcello Ciaccio
OBJECTIVES: Glycated Albumin (GA) has been proposed as a screening marker for diabetes in Asian countries in the last years. Nevertheless, few studies have been conducted in Caucasian population. The aim of this study is to evaluate the clinical usefulness of GA in diabetes diagnosis in Caucasian asymptomatic subjects considered at risk of diabetes based on medical history and Fasting Plasma Glucose (FPG). DESIGN AND METHODS: Three hundred and thirty-four Caucasian subjects having one or more risk factor for diabetes, and/or FPG ranging from 5...
February 24, 2018: Clinical Biochemistry
Douglas F Stickle, Jay H Herman, Ross Molinaro
BACKGROUND: Estimated average glucose (AG) is generally reported along with hemoglobin A1c measurements according to a standard calculation. Given a normal red blood cell lifetime of 120 days, serial A1c measurements at intervals <120 days are not completely independent. For short interval measurements, a change in AG (ΔAG) necessarily underestimates the change in average glucose operative during the interval (ΔG). We use a model for kinetics of HbA1c to evaluate the theoretical relationship between ΔAG and ΔG for HbA1c measurements made at intervals between 0 and 120 days...
February 24, 2018: Clinical Biochemistry
Julia M Kubiak, Guri Grimnes, Kevin D Cashman, Elena Kamycheva, Kirsten Dowling, Zuzana Skrabáková, Rolf Jorde
OBJECTIVE: Evaluate the effects of serum 25-hydroxyvitamin D (25(OH)D) levels, vitamin D binding protein (DBP) and genetic factors on C3-epimerization of 25(OH)D and follow the tracking of the epimer during one year. DESIGN: Cross-sectional and longitudinal study. METHODS: Data from eight previously conducted, Tromsø based studies (3 observational, 5 randomized controlled trials) were combined. 25(OH)D serum samples were re-analyzed with a LC-MS/MS method that also resolves and measures the metabolite C3-epi-25(OH)D3...
February 21, 2018: Clinical Biochemistry
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