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Biochemical Genetics

Valérie Roussel, Alain Van Wormhoudt
The genetic differentiation among the populations of the European abalone Haliotis tuberculata was investigated using different markers to better understand the evolutionary history and exchanges between populations. Three markers were used: mitochondrial cytochrome oxidase I (COI), the sperm lysin nuclear gene, and eight nuclear microsatellites. These markers present different characteristics concerning mutation rate and inheritance, which provided complementary information about abalone history and gene diversity...
October 20, 2016: Biochemical Genetics
Mazen Abuzayed, Nourhan El-Dabba, Anne Frary, Sami Doganlar
Gene diversity (GD), also called polymorphism information content, is a commonly used measure of molecular marker polymorphism. Calculation of GD for dominant markers such as AFLP, RAPD, and multilocus SSRs is valuable for researchers. To meet this need, we developed a free online computer program, GDdom, which provides easy, quick, and accurate calculation of dominant marker GD with a commonly used formula. Results are presented in tabular form for quick interpretation.
October 14, 2016: Biochemical Genetics
Antonis Goulas, Dimitrios Agapakis, Athanassios Apostolidis, Dimitra Gouda, Sotirios Anastassiadis, Christina Trakatelli, Christos Savopoulos, Apostolos I Hatzitolios
Catalase represents perhaps the most effective antioxidant defense in the body under conditions of increased oxidative stress, and rs1001179 (CAT-262C >T) is its most extensively studied gene polymorphism. Using an established PCR-RFLP method for genotyping, we examined the association of rs1001179 with glycated hemoglobin (HbA1c) and plasma lipids using univariate analyses with age, sex, body mass index (BMI), smoking, and alcohol abuse as covariates, in a group of dyslipidemic patients from northern Greece...
October 4, 2016: Biochemical Genetics
Setareh Mirzavand, Karim Sorkheh, Mohammad Reza Siahpoosh
Plant peroxidases belong to a multigene family and possess highly conserved domains allowing designing oligonucleotide primers to amplify DNA sequences coding for peroxidases from plants with unsequenced genomes. Peroxidase gene-based polymorphism among wild Pistacia species, and correlation between peroxidase markers were deduced in this study. Wild Pistacia germplasm was evaluated using 30 peroxidase-specific primers. Targeted-PCR amplification of genomic DNA from 100 wild accessions of Pistacia species produced polymorphisms giving a similarity range from 0...
October 4, 2016: Biochemical Genetics
Atieh Hakimi, Maryam Zolfaghari, Karim Sorkheh
Glycyrrhiza glabra is an endangered and national-protected medicinal plant species distributed in semi-arid and arid areas of South of Iran. This study addresses the genetic diversity and relationship between populations in different habitats by amplified fragment length polymorphism (AFLP). The plant materials consisted of 90 individuals from nine different populating areas of Dezful, Ramhormoz, Ahvaz, Abadan, Khorramshahr, Behbahan, Haft-tapeh, Andimeshk, and Shushtar. Twenty-three AFLP primer combinations generated a total of 1019 bands with 94...
September 28, 2016: Biochemical Genetics
Ozhan Simsek, Pembe Curuk, Fatma Aslan, Melda Bayramoglu, Tolga Izgu, Jaime A Teixeira da Silva, Yildiz Aka Kacar, Yesim Yalcin Mendi
No abstract text is available yet for this article.
September 17, 2016: Biochemical Genetics
Marian Ticlea, Lavinia Melania Bratu, Florian Bodog, Ovidiu Horea Bedreag, Alexandru Florin Rogobete, Zorin Petrisor Crainiceanu
Regarding genetic biomarkers for early assessment and monitoring the clinical course in polytrauma patients with sepsis, in recent years a remarkable evolution has been highlighted. One of the main representatives is the exosome miRNAs. In this paper, we would like to present in more details the various methods of using exosome miRNAs as a biomarker for monitoring polytrauma patients with sepsis, as well as establishing a belated outcome by aggregating the entire clinical aspects. The use of exosome miRNAs for late evaluating and monitoring the clinical evolution of polytrauma patients can bring significant improvements in current clinical practice through the optimization and modulation of intensive care according to the needs of each patient individually...
September 9, 2016: Biochemical Genetics
Ozhan Simsek, Pembe Curuk, Fatma Aslan, Melda Bayramoglu, Tolga Izgu, Jaime A Teixeira da Silva, Yildiz Aka Kacar, Yesim Yalcin Mendi
The genus Cyclamen (family Myrsinaceae) contains about 20 species, most of which occur in the Mediterranean region. Turkey has critically important Cyclamen genetic resources. Molecular characterization of plant materials collected from different regions of Turkey in which Cyclamen species grow naturally, namely Adana, Antalya, Aydın, Muğla, İzmir, Denizli, Kahramanmaraş, Osmaniye, Eskişehir, Trabzon, and Rize provinces, was performed using RAPD and SRAP markers. DNA was successfully amplified by 30 RAPD primers and 14 SRAP primer pairs...
September 7, 2016: Biochemical Genetics
Gulsen Ayaz, Zekai Halici, Abdulmecit Albayrak, Emre Karakus, Elif Cadirci
This study aimed to investigate the effects of the 5-HT7 receptor agonist (LP44) and antagonist (SB269970) on LPS-induced in vivo tissue damage and cell culture by molecular methods. This study was conducted in two steps. For in vivo studies, 24 female rats were divided into four groups. Group I: healthy; II (2nd h): LPS 5 mg/kg administered intraperitoneally (i.p.); III (4th h): LPS 5 mg/kg administered i.p.; IV (8th h): LPS 5 mg/kg administered i.p. For in vitro studies, we used the A549 cell line. Groups: I control (healthy) (2-4 h); II LPS: 1 µg/ml E...
September 1, 2016: Biochemical Genetics
Pui Shen Lau, Kenny Voon Gah Leong, Chin Eng Ong, Amelia Nathania Hui Min Dong, Yan Pan
Cytochrome P450 (CYP) 2C19 is essential for the metabolism of clinically used drugs including omeprazole, proguanil, and S-mephenytoin. This hepatic enzyme exhibits genetic polymorphism with inter-individual variability in catalytic activity. This study aimed to characterise the functional consequences of CYP2C19*23 (271 G>C, 991 A>G) and CYP2C19*24 (991 A>G, 1004 G>A) in vitro. Mutations in CYP2C19 cDNA were introduced by site-directed mutagenesis, and the CYP2C19 wild type (WT) as well as variants proteins were subsequently expressed using Escherichia coli cells...
August 30, 2016: Biochemical Genetics
Mehmet Basbag, Ali Aydin, Muhammet Sakiroglu
Drought is a major stress factor for agricultural production including alfalfa production. One way to counterbalance the yield losses is the introgression of drought tolerant germplasm into breeding programs. As an effort to exploit such germplasm, 16 individual plants were selected from the Southeastern Turkey from their natural habitat and clonally propagated in field trials with an ultimate goal to use the germplasm as parents for releasing a synthetic cultivar. Forage yield and forage quality traits were evaluated and molecular genetic diversity among genotypes were determined using inter simple sequence repeat markers...
August 27, 2016: Biochemical Genetics
Misbah Majid, Akbar Masood, Showkat Ahmad Kadla, Iqra Hameed, Bashir A Ganai
Type 2 diabetes mellitus (T2DM) is characterized by chronic hyperglycemia associated with insulin resistance and relative insulin deficiency. T2DM is believed to be attributable to the combined effect of genetic and environmental factors. Peroxisome proliferator-activated receptor gamma 2 (PPARγ2) is one of the main candidate genes that are implicated in T2DM. A common proline 12 alanine (Pro12Ala) polymorphism in PPARγ2 has been shown to be associated with T2DM. The aim of this work was to investigate the possible role of PPARγ2 gene polymorphism, as a genetic risk factor for T2DM...
August 27, 2016: Biochemical Genetics
Mustafa Akbulut, Mehmet Polat, Sezai Ercisli, Karim Sorkheh
Prunus angustifolia var. angustifolia is a deciduous shrub with 1.5-3.0 m plant height and mostly found in rural areas between 1400 and 1600 m in northeastern Turkey including Bayburt, Gumushane, and Kars provinces. The plant has multistems and well known to adapt severe winter and dry-hot summer conditions. In this study, we aimed to explore the genetic diversity within this specie and also to investigate its phylogenetic relationship with economically important the other cherry species; Prunus avium, Prunus cerasus, Prunus laurocerasus, and Prunus mahaleb...
August 25, 2016: Biochemical Genetics
Júlia Halász, Noémi Makovics-Zsohár, Ferenc Szőke, Sezai Ercisli, Attila Hegedűs
Polyploid Prunus spinosa (2n = 4×) and P. insititia (2n = 6×) represent enormous genetic potential in Central Europe, which can be exploited in breeding programmes. In Hungary, 17 cultivar candidates were selected from wild-growing populations including 10 P. spinosa, 4 P. insititia and three P. spinosa × P. domestica hybrids (2n = 5×). Their taxonomic classification was based on their phenotypic characteristics. Six simple sequence repeats (SSRs) and the multiallelic S-locus genotyping were used to characterize genetic variability and reliable identification of the tested accessions...
August 20, 2016: Biochemical Genetics
Nazila Fathi Maroufi, Khadijeh Farzaneh, Mahdi Alibabrdel, Leila Zarei, Omid Cheraghi, Sina Soltani, Soheila Montazersaheb, Maryam Akbarzadeh, Mohammad Nouri
The metabolic syndrome (MetS) is one of the most important risk factors for type 2 diabetes and cardiovascular disease. This syndrome is characterized by abdominal obesity, hypertension, insulin resistance, and dyslipidemia. The plasma origin of Cholesteryl ester transfer protein (CETP) is responsible for transferring cholesterol esters from high-density lipoprotein particles to apolipoprotein B containing lipoproteins compartment. We conducted this study to investigate the association between CETP gene Taq1B (rs708272) polymorphism in the metabolic syndrome among Iranian subjects...
August 5, 2016: Biochemical Genetics
Amir Nejad-Moghaddam, Sohiela Ajdary, Eisa Tahmasbpour, Farhad Riazi Rad, Yunes Panahi, Mostafa Ghanei
Oxidative stress and inflammation are one of the main pathological consequences of sulfur mustard on human lungs. Unfortunately, there is no effective treatment to mitigate pathological effects of sulfur mustard in mustard lungs. Here, we aimed to evaluate potential efficacy of systemic mesenchymal stem cells administration on expression of oxidative stress- and inflammation-related genes in sulfur mustard-exposed patients. Our patient received 100 million cells per injection, which was continued for four injections within 2 months...
August 2, 2016: Biochemical Genetics
Samet Özer, Resul Yilmaz, Ergün Sonmezgoz, Şeyma Ünuvar, Ömer Ates
Familial Mediterranean fever (FMF) is an autosomal recessively inherited disease characterized by recurrent self-limited attacks of fever accompanied by aseptic inflammation of serosal spaces, joints and skin, peritonitis, pleuritis, and arthritis. Clinical features differ according to genetics variants. The aim of this study was to identify relationship between IL-6 -174G/C gene polymorphisms and clinical features, disease severity score (DSS) and proteinuria in children diagnosed with FMF. In this study, 99 children who were followed-up in Gaziosmanpasa University Medical Faculty Department of Pediatrics and diagnosed with Familial Mediterranean fever according to Tel-Hashomer criteria were included...
July 30, 2016: Biochemical Genetics
İlhan Elmaci, Meric A Altinoz
Pancreatic cancer (PC) and glioblastoma multiforme (GBM) are among the human cancers with worst prognosis which require an urgent need for efficient therapies. Here, we propose to apply to treat both malignancies with a triple combination of drugs, which are already in use for different indications. Recent studies demonstrated a considerable link between risk of PC and diabetes. In experimental models, anti-diabetogenic agents suppress growth of PC, including metformin (M), pioglitazone (P) and lithium (L)...
October 2016: Biochemical Genetics
F Mohseni, Z Rashvand, R Najafipour, Sh Hadizadeh, S Moghbelinejad
Different studies have shown that -238 G>A polymorphism in promoter region of tumor necrosis factor alpha (TNF-α) gene is associated with increased risk of non-alcoholic fatty liver disease (NAFLD). The current study investigates the association between metabolic parameters and nutritional intakes with -238 G>A of TNF-α promoter gene polymorphism among the Iranian patients with NAFLD. In this study, 75 patients with NAFLD and 76 individuals as control were enrolled. Dietary intakes were assessed using a semi-quantitative food-frequency questionnaire...
October 2016: Biochemical Genetics
Muhammad Wasim, Fazli Rabbi Awan, Syeda Sadia Najam, Abdul Rehman Khan, Haq Nawaz Khan
Leptin protein consists of 167 amino acids, which is mainly secreted from the white adipose tissue. This protein acts on the hypothalamic regions of the brain which control eating behavior, thus playing a significant role in maintaining body's metabolism. Leptin receptors belong to glycoprotein 130 (gp130) family of cytokine receptors and exist in six isoforms (LEPR a-f), and all the isoforms are encoded by LEPR gene; out of these isoforms, the LEPR-b receptor is the 'longest form,' and in most of the cases, mutations in this isoform cause severe obesity...
October 2016: Biochemical Genetics
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