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Advances in Experimental Medicine and Biology

Ewelina Witkowska-Sędek, Małgorzata Rumińska, Anna Stelmaszczyk-Emmel, Maria Sobol, Urszula Demkow, Beata Pyrżak
The growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis is involved in the regulation of the receptor activator of nuclear factor kappa B ligand (RANKL)/RANK/osteoprotegerin (OPG) system, but the exact mechanism of the associations is not fully explained. In this study we investigated the serum OPG and total sRANKL concentrations in short children who had differences in GH secretory status. We also investigated the associations between the GH/IGF-1 and OPG/RANKL systems in GH-deficient children during GH treatment...
October 5, 2018: Advances in Experimental Medicine and Biology
M A Rieger, V Liebers, M Nübling, T Brüning, B Brendel, F Hoffmeyer, M Raulf
Acute or chronic inhalation of endotoxin may lead to changes of lung function and inflammatory markers in the airways. Adaptation to workplace exposure may be possible. In this study, we investigated the possible difference in inflammatory markers assessed in nasal lavage fluid (NALF) in chronical exposure compared to voluntary subjects exposed acutely to endotoxin. We sought to define the variability of inflammatory markers in NALF and the dose-related changes after moderate exposure in naïve subjects. Endotoxin exposure (4-1039 EU/m3 ) resulted from routine work during one shift in sewage treatment plants...
October 5, 2018: Advances in Experimental Medicine and Biology
Ewa Rębacz-Maron, Anna Stangret, Iwona Teul
A lower socio-economic status (SES) associates with increased morbidity and mortality. The goal of this study was to define the influence of SES on lung function in indigenous male adolescents (aged 12.8-24.5 years) in Tanzania. The subjects were subdivided into two age groups: <17.5 (n = 189) and ≥ 17.5 years of age (n = 101). They underwent anthropometric and spirometry evaluation. The SES was assessed on the basis of economic family resources and the subjects' nutritional status, and was stratified into three categories: SES-1 (low), SES-2 (average), and SES-3 (high)...
October 2, 2018: Advances in Experimental Medicine and Biology
D Kowalczyk, K Szymański, K Cieślak, E Hallmann-Szelińska, L B Brydak
The diagnostic of influenza virus infections is possible using molecular biology methods as well as the analysis of anti-hemagglutinin (anti-HA) antibodies in the blood serum. The aim of this study was to determine the level of anti-HA antibodies in 7 age groups of patients during the 2015/2016 epidemic season in Poland. A total of 1050 serum samples were tested using the hemagglutination inhibition (HAI) assay. We confirmed the presence of anti-HA antibodies for the influenza virus strains: A/California/7/2009(H1N1)pdm09, A/Switzerland/9715293/2013(H3N2), and B/Phuket/3073/2013, which were the components of the influenza vaccine for the 2015/2016 epidemic season...
October 2, 2018: Advances in Experimental Medicine and Biology
Rolf Hirsch, Jochen Wiesner, Alexander Marker, Armin Bauer, Peter E Hammann, Andreas Vilcinskas
The spread of antibiotic-resistant human pathogens and the declining number of novel antibiotics in the development pipeline is a global challenge that has fueled the demand for alternative options. The search for novel drug candidates has expanded to include not only antibiotics but also adjuvants capable of restoring antibiotic susceptibility in multidrug-resistant (MDR) pathogens. Insect-derived antimicrobial peptides (AMPs) can potentially fulfil both of these functions. We tested two coleoptericins and one coleoptericin-like peptides from the invasive harlequin ladybird Harmonia axyridis against a panel of human pathogens...
September 30, 2018: Advances in Experimental Medicine and Biology
Małgorzata Wojciechowska, Maciej Zarębiński, Piotr Pawluczuk, Dagmara Gralak-Łachowska, Leszek Pawłowski, Wioletta Loska, Marzena Goszczyńska, Krzysztof Flis, Agnieszka Cudnoch-Jędrzejewska
Remote ischemic preconditioning (RIPC) exerts protection in remote organs. The purpose of this study was to investigate the potential of RIPC to prevent contrast induced nephropathy. One hundred and twenty four patients were randomized to elective percutaneous coronary intervention with or without RIPC. RIPC was performed using three cycles of 5-min inflation to 200 mmHg of a standard upper arm blood pressure cuff. The time between the last inflation cycle and the coronary intervention was less than 2 h. The primary endpoint was the incidence of contrast-induced nephropathy based on the standard criteria of the serum creatinine (SC) and cystatin C (CC) levels...
September 29, 2018: Advances in Experimental Medicine and Biology
Fatma Betül Ayanoğlu, Ayşe Eser Elçin, Yaşar Murat Elçin
Quantitative real-time polymerase chain reaction (qRT-PCR) is a widely utilized method for evaluating the gene expressions in stem cell research. This method enables researchers to obtain fast and precise results, but the accuracy of the data depends on certain factors, such as those associated with biological sample preparation and PCR efficiency. In order to achieve accurate and reliable results, it is of utmost importance to designate the reference genes, the expressions of which are suitable to all kinds of experimental conditions...
September 29, 2018: Advances in Experimental Medicine and Biology
Iwona Stanisławska, Bożena Witek, Marek Łyp, Danuta Rochon-Szmejchel, Adam Wróbel, Wojciech Fronczyk, Agnieszka Kamińska, Adam Kołątaj, Daniel Załuski
In this study, the effect of glutathione (GSH) on the activity of hydrolytic enzymes of lysosomal, microsomal, and cytosolic fractions was studied in the mouse hepatocytes. The experiments involved 30 Swiss male mice, divided into the experimental and control groups of 15 mice each. The former group received injections of 12 μL/g of GSH solution at a dose of 100 μg/g body weight, whereas the latter received 12 μL/g of physiological saline, all given intraperitoneally daily for 7 days. Then, fragments of liver tissue were collected from the euthanized animals and processed to obtain lysosomal, microsomal, and cytosolic fractions of hepatocytes...
September 29, 2018: Advances in Experimental Medicine and Biology
Benjamin E Turk
The zinc-dependent metalloprotease anthrax lethal factor (LF) is the enzymatic component of a toxin thought to have a major role in Bacillus anthracis infections. Like many bacterial toxins, LF is a secreted protein that functions within host cells. LF is a highly selective protease that cleaves a limited number of substrates in a site-specific manner, thereby impacting host signal transduction pathways. The major substrates of LF are mitogen-activated protein kinase kinases (MKKs), which lie in the middle of three-component phosphorylation cascades mediating numerous functions in a variety of cells and tissues...
September 29, 2018: Advances in Experimental Medicine and Biology
Patrycja Naumczyk, Angelika K Sawicka, Beata Brzeska, Agnieszka Sabisz, Krzysztof Jodzio, Marek Radkowski, Karolina Czachowska, Paweł J Winklewski, Karolina Finc, Edyta Szurowska, Urszula Demkow, Arkadiusz Szarmach
This study seeks to define the role of predictive values of the motor speed, inhibition control, and fluid and crystallized intelligence in estimating the cortical thickness in healthy elderly. Forty-six older healthy subjects (37 women, 9 men) over 60 years of age were included in the study. The participants were examined on 3.0 T MRI scanners. The protocol included standard anatomical sequences, to exclude brain pathology, and a high-resolution T1-weighted sequence used to estimate the cortical thickness...
September 29, 2018: Advances in Experimental Medicine and Biology
Iwona Patyk, Cezary Rybacki, Agata Kalicka, Agnieszka Rzeszotarska, Jolanta Korsak, Andrzej Chciałowski
Chronic obstructive pulmonary disease (COPD) is a progressive disease underlain by airway inflammation. Despite trials with new generations of anti-inflammatory drugs to alleviate the disease burden, the effective curative treatment remains elusive. In this context, the aim of this study was to assess the influence of simvastatin, a leading member of the family of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, known to display anti-inflammatory and immunomodulatory activity, on symptoms and lung function, as well as the proportion of inflammatory cells, cytokines, proteolytic enzymes, and surfactant protein D (SP-D) content in bronchoalveolar lavage fluid (BALF) in COPD patients...
September 26, 2018: Advances in Experimental Medicine and Biology
M Wojda, J Kostrzewa-Janicka, P Śliwiński, P Bieleń, P Jurkowski, R Wojda, E Mierzwińska-Nastalska
Obstructive sleep apnea (OSA) is defined as episodes of upper airway obstruction occurring during sleep. Conservative treatment of OSA consists of continuous positive airway pressure (CPAP). An alternative treatment in mild-to-moderate OSA could be the use of intraoral mandibular advancement devices (MAD). The aim of this study was to evaluate therapeutic efficacy of MAD in OSA patients intolerant to CPAP. The study group included 8 patients, who fulfilled specific inclusion criteria during a dental examination, out of the 30 CPAP intolerant patients who were referred for the possible use of MAD...
September 26, 2018: Advances in Experimental Medicine and Biology
Esra Aydemir Çoban, Fikrettin Şahin
Tumors consists of subpopulation of cells in which each subtype has contributes to tumor progression. Specifically one subtype known as cancer stem cells are associated with the initiation, progression, resistance to conventional therapies and metastasis. Metastasis is leading cause of cancer related deaths. Overall it is important to consider cancer as a whole in which a mutated cell proliferating indefinitely and forming its hierarchy consisting of subgroups with different molecular signatures. To be able to target this disease we need to evaluate every step including initiation, progression, survival, angiogenesis and finally migration and repopulation...
September 26, 2018: Advances in Experimental Medicine and Biology
A Płóciniczak, A Baszczuk, A Ludziejewska, H Winiarska, S Michalak, G Kasprzak, D Formanowicz, S Cofta, E Wysocka
The antioxidant enzyme paraoxonase-1 (PON1) may limit oxidative stress in the development of cardiovascular diseases (CVD) and obstructive sleep apnea (OSA). The aim of the study was to determine PON1 gene L55M polymorphism in OSA-positive and OSA-negative subjects, along with paraoxonase activity of the enzyme (PON1-act). Caucasians aged 25-75, with BMI 19.0-53.0 kg/m2 and no acute or severe chronic disorder underwent polysomnography, and OSA-negative (n = 44) and OSA-positive (n = 57) groups were established...
September 23, 2018: Advances in Experimental Medicine and Biology
Wojciech Krajewski, Urszula Halska, Sławomir Poletajew, Radosław Piszczek, Bartosz Bieżyński, Mateusz Matyjasek, Andrzej Tukiendorf, Fryderyk Menzel, Małgorzata Mazur, Joanna Rymaszewska, Romuald Zdrojowy
The standard of care in non-muscle invasive bladder cancer consists of transurethral tumor resection. The aim of this study was to evaluate the influence of transurethral resection of bladder tumor (TURB) on the patients' self-reported depression, anxiety, sexual satisfaction, and erectile dysfunction. Psychological condition of 252 male patients who underwent TURB was prospectively evaluated. The Hospital Anxiety and Depression Scale (HADS), simplified International Index of Erectile Function (IIEF-5), and Sexual Satisfaction Questionnaires (SSQ) were administered to patients before and 10 days after tumor resection...
September 23, 2018: Advances in Experimental Medicine and Biology
Maciej Grzywnowicz, Emilia Majsiak, Józef Gaweł, Karolina Miśkiewicz, Zbigniew Doniec, Ryszard Kurzawa
Despite being clinically largely irrelevant, antibodies against cross-reactive carbohydrate determinants (CCD) are an important issue in the in vitro diagnostics, as they may produce false positive or falsely elevated results of the immunoglobulin E class (asIgE) in relation to the actually present level of asIgE. The present chapter demonstrates an effective resolution of this diagnostic issue by the use of a CCD inhibitor in in vitro tests. A synthetic CCD inhibitor, Polycheck® CCD inhibitor, was used in the laboratory diagnostics of 24 children diagnosed with allergic diseases...
September 23, 2018: Advances in Experimental Medicine and Biology
Christian Monsé, Birger Jettkant, Bernd Karl Heinrich Schramm, Horst Christoph Broding, Matthias Knappe, Manfred Michl, Frank Hoffmeyer, Kirsten Sucker, Thomas Brüning, Jürgen Bünger
Potash miners can become exposed to carbon dioxide (CO2 ) during the blasting of basalt intrusions or loading and transporting the blasted salt. In a cross-shift study, we compared physiological effects of acute exposure to elevated CO2 concentrations in miners after long-term exposure to evaluate the possible health risks. A group of 119 miners was assessed by clinical examination, lung function tests, and blood gas content directly before and after the shift. A cumulative CO2 exposure was measured using personal monitors...
September 22, 2018: Advances in Experimental Medicine and Biology
Nhat Chau Truong, Khanh Hong-Thien Bui, Phuc Van Pham
INTRODUCTION: Since the 1980s, adipose-derived stem cells (ASCs) have become a powerful and potential source for stem cell-based therapy, regenerative medicine, and even drug delivery in cancer treatment. The development of off-the-shelf mesenchymal stem cells (MSCs), including ASCs, has rapidly advanced in recent years with several clinical trials and approved products. In this technology, ASCs should be expanded long term in order to harvest higher cell number. In this study, senescence of ASCs after long-term expansion was evaluated...
September 22, 2018: Advances in Experimental Medicine and Biology
E Hallmann-Szelińska, K Cieślak, K Szymański, D Kowalczyk, M R Korczyńska, I Paradowska-Stankiewicz, L B Brydak
In the influenza season 2016/2017 in Europe, the predominant virus was A/H3N2/. In Poland, the percentage of people vaccinated against influenza was 3.33%. European I-MOVE+ project shows how important it is to monitor the effectiveness of influenza vaccine. The project demonstrates that a match between the circulating vaccine strains and those included in the vaccine for the Northern Hemisphere was low-to-moderate. In the present study, there were 379 patients and 296 control subjects examined in hospitals in Poland as part of I-MOVE+ project...
September 11, 2018: Advances in Experimental Medicine and Biology
Ali Golchin, Mahmoud Rekabgardan, Ramezan Ali Taheri, Mohammad Reza Nourani
Regenerative medicine (RM) is a promising new field of medicine that has mobilized several new tools to repair or replace lost or damaged cells or tissues by stimulating natural regenerative mechanisms nearby cell and tissue-based therapy approaches. However, mesenchymal stem cell (MSC) based therapy has been shown to be safe and effective to a certain degree in multiple clinical trial studies (CTSs) of several diseases, in most MSC CTSs the efficacy of treatment has been reported low. Therefore, researchers have focused on efficacy enhancing of MSC to improve migratory and homing, survival, stemness, differentiation and other therapeutic applicable properties by using different approaches...
August 29, 2018: Advances in Experimental Medicine and Biology
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