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Archives of Oral Biology

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https://www.readbyqxmd.com/read/30025275/correlation-between-crestal-alveolar-bone-loss-with-intracanal-bacteria-and-apical-lesion-area-in-necrotic-teeth
#1
Francisco Wilker M G Muniz, Francisco Montagner, Rogério C Jacinto, Cassiano K Rösing, Brenda P F A Gomes
OBJECTIVE: This study aimed to analyze the correlation between crestal alveolar bone loss with the presence of some bacterial species in root canals and the apical lesion area of necrotic teeth. DESIGN: Data from 20 patients with diagnosis of pulp necrosis and acute apical abscesses, without active periodontal diseases, were evaluated. Patients with history of antibiotic usage three months prior to the study, with exposed pulp cavity, and with probing depth >3 mm were not included...
July 11, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/30031211/influence-of-chronic-alcoholism-and-estrogen-deficiency-on-the-immunohistochemical-expression-of-regulatory-proteins-of-the-bone-resorption-process-in-the-periodontium-of-wistar-rats
#2
Adriana Mathias Pereira de Silva Marchini, Gabriela de Fátima Santana-Melo, Miriane Carneiro Machado Salgado, Elis Andrade de Lima Zutin, Leonardo Marchini, Rosilene Fernandes da Rocha, Luana Marotta Reis de Vasconcellos
OBJECTIVE: The aim of this study was to investigate possible changes in immunohistochemical expression of proteins regulating the bone resorption process in the periodontium of rats subjected to alcoholism and/or estrogen deficiency. The investigated proteins were receptor activator of nuclear factor-kappa β ligand (RANKL), a protein that stimulates bone resorption, and osteoprotegerin (OPG), a protein that inhibits bone resorption. At the molecular level, decreased OPG expression and/or increased RANKL expression are consistent with a greater predisposition to bone resorption...
July 10, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/30015218/effect-of-chitosan-nanoparticles-on-the-inhibition-of-candida-spp-biofilm-on-denture-base-surface
#3
Brenna Louise Cavalcanti Gondim, Lúcio Roberto Cançado Castellano, Ricardo Dias de Castro, Giovanna Machado, Hugo Lemes Carlo, Ana Maria Gondim Valença, Fabíola Galbiatti de Carvalho
OBJECTIVES: Chitosan nanoparticles (ChNPs) have antifungal effects, however there is a lack of information about the effects of ChNPs against Candida biofilm on denture base surface. This study investigated the ChNPs effect against C. albicans biofilm adhesion and formation, and against Candida spp. biofilm on heat-cured acrylic resin. DESIGN: The ChNPs were synthetized (3800 μg/mL) and characterized by infra-red spectrophotometry and transmission electron microscopy...
July 9, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/30015217/in-silico-analysis-of-virulence-genes-in-an-emerging-dental-pathogen-a-baumannii-and-related-species
#4
J Vijayashree Priyadharsini, A S Smiline Girija, A Paramasivam
OBJECTIVES: Acinetobacter baumannii is an opportunistic pathogen which has recently been categorized as a high risk pathogen by World Health Organisation (WHO). The microbe has stealthily entered the oral cavity and has established itself as a potential pathogen by acquiring drug resistance and expression of several virulence genes. Surveillance on the type of virulence factors harboured by the organism will enable us to comprehend the mechanism of pathogenesis. The study was performed to screen for the presence of crucial virulence factors associated with Acinetobacter spp...
July 7, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29990588/investigation-on-the-relationship-of-dimensions-of-the-maxillary-sinus-drainage-system-with-the-presence-of-sinusopathies-a-cone-beam-computed-tomography-study
#5
Ana Beatriz Gomes de Carvalho, Andre Luiz Ferreira Costa, Acácio Fuziy, Afonso Celso Souza de Assis, José Ribamar Castro Veloso, Luiz Roberto Coutinho Manhães, Mauro Pedrine Santamaria, Sérgio Lucio Pereira de Castro Lopes
OBJECTIVE: This study sought to assess the relationship between the dimensions of the maxillary sinus drainage system with the content of sinuses. DESIGN: Three-hundred cone beam computed tomography images were selected from a database (105 males and 195 females). The images were assessed by a single investigator, trained and calibrated, performing image analysis. The length of the infundibulum and the ostium height in both maxillary sinuses were measured. The data were analyzed using Minitab 1, using 5% as a critical p-value...
July 3, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29990589/symmetry-of-root-anatomy-and-root-canal-morphology-in-maxillary-premolars-analyzed-using-cone-beam-computed-tomography
#6
Yi-Han Li, Shi-Jie Bao, Xiang-Wen Yang, Xiao-Mei Tian, Bin Wei, Yuan-Li Zheng
OBJECTIVE: This study aimed to use cone-beam computed tomography (CBCT) to evaluate the root anatomy and canal morphology of maxillary premolars in a Chinese population and determine their degree of bilateral symmetry. DESIGN: 774 CBCT images were retrospectively analyzed, representing 1387 maxillary first premolars and 1403 second premolars. The number of roots and canals were recorded. The morphology of root canal systems was determined according to Vertucci's classification...
June 28, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29979975/inhibitory-effects-of-tea-catechin-epigallocatechin-3-gallate-against-biofilms-formed-from-streptococcus-mutans-and-a-probiotic-lactobacillus-strain
#7
Ching-Yi Wu, Tzu-Yi Su, Mao-Yu Wang, Shue-Fen Yang, Kwei Mar, Shan-Ling Hung
OBJECTIVE: Effects of tea catechin epigallocatechin-3-gallate (EGCG) against biofilm formation by Streptococcus mutans and probiotic Lactobacillus casei in Yakult® (LcY) were examined. DESIGN: Biofilms were formed by S. mutans alone (Sm) and co-culture of S. mutans and LcY (Sm + LcY) in the absence or presence of EGCG. The biomass of biofilms, which were sonicated or not, was measured by the crystal violet assay. Biofilm morphology was observed by scanning electron microscopy...
June 26, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29957456/effects-of-anterior-tooth-crown-inclination-on-occlusal-relationship-a-study-in-three-dimensional-reconstruction
#8
Feng Feng, Yifan Liu, Jingwen Chi, Yuqiao Wang, Bin Xing, Yucheng Wang, Wen Liu
OBJECTIVE: This study aims to analyze the relationship between the central incisor crown inclination and occlusal relationship via three-dimensional study. METHODS: Ten Chinese volunteers with normal occlusion were selected from physical examination of college students. Lateral cephalograms and CT scans were taken, and the CT data were imported into Simplant pro 11.04 to reconstruct three-dimensional model. In the three-dimensional model, every tooth was separated independently...
June 21, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29945092/epigallocatechin-3-gallate-reduces-damage-to-osteoblast-like-cells-treated-with-zoledronic-acid
#9
Eduardo Pons-Fuster López, Juan Seoane Leston, Pia López Jornet
OBJECTIVE: analyze the effect of zoledronic acid (ZA) alone and in combination with epigallocatechin-3-gallate (EGCG) on human osteoblast cells. METHODS: SAOS-2 osteoblastic phenotype cell line treated with different doses (0.01-10 μM) of ZA or ZA + EGCG underwent cell viability, mineralization, collagen synthesis and cell migration tests. RESULTS: Micromolar (1 and 5 μM) doses of ZA reduced cell viability (p < 0.001), mineralization (p < 0...
June 20, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29957455/the-impact-of-dietary-consistency-on-structural-craniofacial-components-temporomandibular-joint-condyle-condylar-cartilage-alveolar-bone-and-periodontal-ligament-a-systematic-review-and-meta-analysis-in-experimental-in-vivo-research
#10
REVIEW
Rolf Scheidegger, Despina Koletsi, Theodore Eliades
OBJECTIVE: The aim of this systematic review was to provide a comprehensive synthesis of available evidence evaluating the effect of dietary loading on temporomandibular joint/condyle, condylar cartilage, alveolar bone of the mandible and the periodontal ligament in healthy mice and rats. DESIGN: Medline via PubMed, EMBASE and Open Grey databases were searched for published and unpublished literature. Search terms included "mandiblular condyle", "alveolar bone", "temporomandibular joint", "condylar cartilage", "periodontal ligament", "rat", "mice"...
June 19, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29929068/are-hertwig-s-epithelial-root-sheath-cells-necessary-for-periodontal-formation-by-dental-follicle-cells
#11
Yongwen Guo, Weihua Guo, Jie Chen, Guoqing Chen, Weidong Tian, Ding Bai
OBJECTIVE: The role of Hertwig's epithelial root sheath (HERS) cells in periodontal formation has been controversial. This study aimed to further clarify whether HERS cells participate in formation of the periodontium, and the necessity of HERS cells in differentiation of dental follicle cells (DFCs) for periodontal regeneration. DESIGN: HERS cells and DFCs were isolated and identified from post-natal 7-day Sprauge-Dawley rats. In vitro, direct co-culture of HERS cells and DFCs as well as the individual culture of HERS and DFCs were performed and followed by alizarin red staining and the quantitative real-time polymerase chain reaction analysis...
June 18, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29933139/effects-of-citric-acid-modified-with-fluoride-nano-hydroxyapatite-and-casein-on-eroded-enamel
#12
Ayşe Dündar, Abdulkadir Şengün, Canan Başlak, Mahmut Kuş
OBJECTIVES: The aim of this in vitro study was to evaluate the effects of citric acid containing fluoride, nano-hydroxyapatite, and casein on eroded enamel. DESIGN: The crowns of 120 extracted bovine incisors were embedded in acrylic resin. An enamel window (2 × 3 mm) was created on the surface. Before in vitro pellicle formation samples were eroded in 1% citric acid (pH = 3.2) for 1 h at 36 °C and were randomly classified to eight groups (n = 15) as follows: Positive control: 1% citric acid, Negative control: Distilled water, F1: 0...
June 15, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29929069/a-novel-electron-microscopic-method-for-measurement-of-mineral-content-in-enamel-lesions
#13
C Fowler, R J M Lynch, D Shingler, D Walsh, C Carson, A Neale, R J Willson, A Brown
OBJECTIVE: To assess Scanning Electron Microscopy in Back-Scattered Emission mode (BSE-SEM) for measurement of lesion mineral content as a function of depth. Direct comparison is made with Transverse Micro-Radiography (TMR) and Surface Micro-Hardness (SMH) on carious and erosive lesions. DESIGN: Caries lesions prepared from sound bovine enamel at 37 °C and pH 4.6 in unsaturated (7d) or part-saturated (8d, 4.1 mM Ca2+ , 8 mM Pi) lactic acid /methyl cellulose gel system, followed by TMR analysis...
June 13, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29929057/serotype-a-of-aggregatibacter-actinomycetemcomitans-down-regulates-the-increased-serotype-b-induced-cytokine-and-chemokine-production-in-dendritic-cells
#14
Leticia Rojas, Samanta Melgar-Rodríguez, Jaime Díaz-Zúñiga, Carla Alvarez, Gustavo Monasterio, Carolina Rojas, Paola Carvajal, Rolando Vernal
OBJECTIVE: In Aggregatibacter actinomycetemcomitans, different serotypes have been described based on LPS antigenicity. Mixed infection with the different A. actinomycetemcomitans serotypes is frequent in periodontitis patients; accordingly, the role of this bacterial species in the pathogenesis of periodontitis may differ depending whether patients or periodontal lesions harbour one or more of the A. actinomycetemcomitans serotypes. We hypothesized that different combinations of these serotypes could be associated with distinct host responses and hence different inflammatory patterns...
June 12, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29979974/effects-of-far-infrared-radiation-by-isotropic-high-density-carbon-on-the-human-oral-mucosa
#15
Isao Kimura, Toshiro Yamamoto, Koya Nakamura, Toshihiro Uenishi, Taku Asai, Masakazu Kita, Narisato Kanamura
OBJECTIVES: Wound healing of the oral mucosal epithelium through the application of far infrared radiation emitted by isotropic high-density carbon was investigated in order to clarify the preventive and therapeutic effects of isotropic high-density carbon on oral mucosal injury. MATERIALS AND METHODS: A carbon massager with an isotropic high-density carbon tip was used. Far infrared radiation was applied to the human buccal mucosal squamous cell carcinoma cell line, HO-1-N-1 using a carbon massager, and cell growth factors and heat shock protein levels were measured using real-time RT-PCR and ELISA...
June 11, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29929070/effects-of-caffeic-acid-phenethyl-ester-application-on-dentin-mmp-2-stability-of-bond-strength-and-failure-mode-of-total-etch-and-self-etch-adhesive-systems
#16
Vivianne Oliveira Pedrosa, Fabiana Mantovani Gomes França, Cecilia Pedroso Turssi, Flávia Lucisano Botelho do Amaral, Lucas Novaes Teixeira, Elizabeth Ferreira Martinez, Roberta Tarkany Basting
OBJECTIVE: Investigate the long-term effect of dentin pretreatment with 0.05 or 0.1% caffeic acid phenethyl ester (CAPE) on (1) bond strength of resin composite to dentin by a three-step etch-and-rinse (Adper Scotchbond Multipurpose/ ASB) or a two-step self-etch adhesive system (Clearfil SE Bond/ CSE), (2) their fracture mode, (3) the micromorphological features of the hybrid layer formed; and (4) the level of MMP-2 in dentin (after application, using a correlative immunoexpression/quantification approach)...
June 11, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29929058/responses-to-spaceflight-of-mouse-mandibular-bone-and-teeth
#17
Didem Dagdeviren, Zana Kalajzic, Douglas J Adams, Ivo Kalajzic, Alan Lurie, Maija I Mednieks, Arthur R Hand
OBJECTIVE: To determine if spaceflight and microgravity affect non-weight bearing bones and development and mineralization of teeth, reasoning that combining an organ and a cellular level approach can lead to greater insights about these effects. DESIGN: Mandibles and incisors of mice flown on the US STS-135 space shuttle mission and the Russian Bion-M1 satellite were studied using micro-computed tomography and immunohistochemistry. Ground controls were mice housed in standard vivarium cages and flight habitats...
June 7, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29960917/identification-of-major-matrix-metalloproteinase-20-proteolytic-processing-products-of-murine-amelogenin-and-tyrosine-rich-amelogenin-peptide-using-a-nuclear-magnetic-resonance-spectroscopy-based-method
#18
Garry W Buchko, Rajith Jayasinha Arachchige, Jinhui Tao, Barbara J Tarasevich, Wendy J Shaw
OBJECTIVE: The aim of this study was to identify major matrix metalloproteinase-20 (MMP20) proteolytic processing products of amelogenin over time and determine if the tyrosine-rich amelogenin peptide (TRAP) was a substrate of MMP20. DESIGN: Recombinant15 N-labeled murine amelogenin and 13 C,15 N-labeled TRAP were incubated with MMP20 under conditions where amelogenin self-assembles into nanospheres. Digestion products were fractionated by reverse-phase high-performance liquid chromatography at various time points...
June 6, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29929056/n-acetyl-cysteine-inhibits-lipopolysaccharide-mediated-induction-of-interleukin-6-synthesis-in-mc3t3-e1-cells-through-the-nf-kb-signaling-pathway
#19
Ling Guo, Hui Zhang, Wangyang Li, Danting Zhan, Min Wang
BACKGROUND: Interleukin-6 (IL-6) is a potent stimulator of osteoclastic activity. Lipopolysaccharide (LPS) has been shown to regulate the expression of potent inflammatory factors, including TNF-α and IL-6. Currently, effective therapeutic treatments for bacteria-caused bone destruction are limited. N-acetyl cysteine (NAC) is an antioxidant small molecule that possibly modulates osteoblastic differentiation. However, whether NAC can affect the LPS-mediated reduction of IL-6 synthesis in MC3T3-E1 cells is still unknown...
June 6, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29913322/culture-supernatants-of-oral-cancer-cells-induce-impaired-ifn-%C3%AE-production-of-pdcs-partly-through-the-down-regulation-of-tlr-9-expression
#20
Nannan Han, Zun Zhang, Houyu Jv, Jingzhou Hu, Min Ruan, Chenping Zhang
OBJECTIVES: The aim of the present study was to investigate whether tumor-derived supernatants down-regulate the immune function of plasmacytoid dendritic cells (pDCs) in oral cancer and the potential molecular mechanisms of this effect. MATERIALS AND METHODS: Immunohistochemistry (IHC) and flow cytometry were used to detect tumor-infiltrating and peripheral blood pDCs. MTS and flow cytometry were employed to evaluate the immune response of CD4+ T cells. Real-time PCR and ELISA assays were used to identify TLR-7 and TLR-9 expression, IFN-α production and tumor-secreted soluble cytokines...
June 5, 2018: Archives of Oral Biology
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