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Archives of Oral Biology

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https://www.readbyqxmd.com/read/28092867/expression-of-mmp-2-and-timp-1-during-rapid-maxillary-expansion-in-rats
#1
Jianwei Chen, Jing Zhou, Fan Li, Jianfeng Sun, Guifeng Li, Shujuan Zou, Qingsong Ye
OBJECTIVE: The aim of this study was to investigate the expression of MMP-2 and TIMP-1 during midpalatal suture expansion in rats. DESIGN: 72 male Wistar rats were randomly divided into 2 groups: the experimental group and the control group. In the experimental group, opening loops were applied across the midpalatal suture with an initial force of 50g, whereas in the control group, rats were subjected to sham installation of opening loops without activation. On day 1, 4, 7 and 14, nine rats from each group were sacrificed, and the maxillae were dissected and prepared for Immunohistochemistry (IHC) and RT- PCR examination of MMP-2 and TIMP-1 expression...
January 4, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28086152/time-dependent-deformation-behavior-of-dentin
#2
C Montoya, D Arola, E A Ossa
OBJECTIVE: The viscoelastic behavior of dentin and its ability to undergo time dependent deformation are considered to be important to oral functions and its capacity to resist fracture. There are spatial variations in the microstructure of dentin within the crown, which could be important to the viscous behavior. However, a spatially resolved description for the viscoelastic behavior of coronal dentin has not been reported. METHODS: In this investigation spherical indentations were made in three regions of coronal dentin including the outer, middle and inner regions...
January 4, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28063305/inhibitory-effects-of-lactobacillus-rhamnosus-and-lactobacillus-casei-on-candida-biofilm-of-denture-surface
#3
Young-Gyun Song, Sung-Hoon Lee
OBJECTIVE: Candida albicans biofilm is associated with denture-related stomatitis and oral candidiasis of elderly. Probiotics are beneficial bacteria and have antibacterial activity against pathogenic bacteria. The purpose of this study was to investigate the antifungal activity of various probiotics against C. albicans and the inhibitory effects of probiotics on Candida biofilm on the denture surface. DESIGN: The spent culture media of various probiotics were investigated the antifungal efficacy against C...
December 31, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28068523/rs12941170-at-sox9-gene-associated-with-orofacial-clefts-in-chinese
#4
Zhong-Lin Jia, Sha He, Shu-Yuan Jiang, Bi-He Zhang, Shi-Jun Duan, Jia-Yu Shi, Ning Huang, Wen-Chao Zhu, Bing Shi
OBJECTIVE: Non-syndromic orofacial cleftings (NSOCs) are considered as complex trait, which results from genetic and/or environmental modifiers. Current findings could only explain small portion of the NSOCs. SOX9 gene plays an important role during craniofacial development in animal models and the Pierre Robin sequence (PRS). However, its role in non-syndromic clefts remains unknown. DESIGN: In this study, we selected eight SNPs in and around SOX9 gene to make maximum coverage, and genotyped them by using RFLP-PCR and ligase detection reaction (LDR) methods to test its associations among 151 NSOCs (53 NSCLP, 52 NSCLO and 46 NSCPO) from Western Han Chinese population...
December 30, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28068524/enzyme-activities-in-parotid-saliva-of-patients-with-the-restrictive-type-of-anorexia-nervosa
#5
Elzbieta Paszynska, Agnieszka Slopien, Monika Dmitrzak-Weglarz, Christian Hannig
OBJECTIVE: In patients with anorexia nervosa (AN) specific signs may occur in the oral cavity, but there are conflicting reports about their significance, especially concerning changes in salivary composition. The aim of this clinical study was to evaluate the resting parotid flow rate (PFR) and the activity of the following enzymes in parotid saliva: amylase, aspartate amino transferase (AST), lysozyme, peroxidase, serine and acidic proteases in the acute phase of the restrictive type of AN and to compare the findings with those in healthy controls...
December 29, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28043014/expression-of-matrix-metalloproteinases-2-9-and-reversion-inducing-cysteine-rich-protein-with-kazal-motifs-in-gingiva-in-periodontal-health-and-disease
#6
Nian Liu, Yingguang Cao, Guangxun Zhu
BACKGROUND AND OBJECTIVE: Periodontitis is characterized by pathological destruction of extracellular matrix (ECM) of periodontal tissues. Matrix metalloproteinases (MMPs) promote the occurrence and development of periodontitis by degrading almost all proteins of ECM. RECK (reversion-inducing-cysteine-rich protein with kazal motifs), a novel membrane-anchored inhibitor of MMPs, could regulate the expression of MMP-2 and MMP-9 at post-transcriptional level. The study was to investigate the expression of RECK in healthy and diseased human gingival tissues and to correlate it with the production of MMP-2 and MMP-9...
December 26, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28043013/transient-receptor-potential-melastatin-trpm-8-is-expressed-in-freshly-isolated-native-human-odontoblasts
#7
Kento Tazawa, Hideharu Ikeda, Nobuyuki Kawashima, Takashi Okiji
OBJECTIVE: Cold-sensitive ion channels, such as transient receptor potential melastatin (TRPM) 8 and transient receptor potential ankyrin (TRPA) 1, may play a crucial role in the nociceptive function of odontoblasts, whereas expression of these TRP channels in human native odontoblasts remains to be elucidated. This study aimed to analyze the expression of TRPM8 and TRPA1 in freshly isolated native human odontoblasts. DESIGN: Odontoblasts were isolated from freshly extracted healthy human teeth (n=4); after removing the inner pulp tissues from the pulp chambers, odontoblasts remaining on the dentin surface were washed out with phosphate buffered saline and collected...
December 23, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28040606/steinert-syndrome-and-repercussions-in-dental-medicine
#8
REVIEW
Helena Baptista, Inês Lopes Cardoso
Steinert syndrome, also called myotonic dystrophy type 1, is a genetic disorder with autosomal dominant transmission characterized by myotonia and a multisystemic clinical picture that affects several tissues of the human body. The most common systemic phenotypes are: muscular, cardiac, respiratory, CNS, ocular, gynecological, digestive, orthopedical, as well as cognitive and psychological symptoms (cognitive decline). Muscles involved in voluntary movement are highly affected by myotonia especially distal muscles of upper limbs...
December 23, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28043012/tnf-alpha-stimulation-increases-dental-pulp-stem-cell-migration-in-vitro-through-integrin-alpha-6-subunit-upregulation
#9
Lei Shi, Shanqi Fu, Sidra Fahim, Shuang Pan, He Lina, Xiaodan Mu, Yumei Niu
OBJECTIVE: The dissemination of stem cells into tissues requiring inflammatory and reparative response is fundamentally dependent upon their chemotactic migration. Expression of TNF-α is up regulated in inflamed pulps. Dental pulp cells are also known to express integrin α6 subunit. Expression of integrin subunit α6 has been linked to the acquisition of migratory potential in a wide variety of cell types in both pathological and physiological capacities. Therefore, in this study we examined the effects of a pleiotropic cytokine TNF-α on the migration of hDPSCs and investigated its relationship with expression of integrin α6 in hDPSCs during chemotactic migration...
December 15, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27978477/only-severe-malocclusion-correlates-with-mastication-deficiency
#10
Pierre Bourdiol, Delphine Soulier-Peigue, Pauline Lachaze, Emmanuel Nicolas, Alain Woda, Martine Hennequin
OBJECTIVES: The relation between level of dentofacial deformity and extent of masticatory deficiency was studied. DESIGN: Three groups of human young adults were formed: (i) subjects needing orthodontics plus orthognathic surgery (SevDFD, n=18), (ii) subjects needing orthodontic treatment only (ModDFD, n=12), and (iii) subjects needing no treatment (NoDFD, n=12). For mastication tests, carrot boluses were collected at the deglutition time. Bolus particle size range was expressed as d50 value, which was compared with the Masticatory Normative Indicator (MNI)...
December 8, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27951410/non-syndromic-severe-hypodontia-caused-by-a-novel-frameshift-insertion-mutation-in-the-homeobox-of-the-msx1-gene
#11
Mushriq F Abid, M A Simpson, Christos Petridis, M T Cobourne, P T Sharpe
OBJECTIVE: Inherited congenital anomalies in tooth number, particularly hypodontia are relatively common. Although substantial progress has been made that permits a better understanding of the causes of tooth agenesis, overall knowledge of the phenotype:genotype correlations in this anomaly are still lacking. The aim in this study was to identify the causal gene mutation(s) in a family of two sisters with severe hypodontia (oligodontia) including 2nd premolars and 1st and 3rd molars, using whole exome sequencing (WES)...
December 6, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28012366/influence-of-low-level-laser-therapy-on-the-healing-process-of-autogenous-bone-block-grafts-in-the-jaws-of-systemically-nicotine-modified-rats-a-histomorphometric-study
#12
Juliano Milanezi de Almeida, Ricardo Oliveira de Moraes, David Jonathan Rodrigues Gusman, Paula Lazilha Faleiros, Maria José Hitomi Nagata, Valdir Gouveia Garcia, Letícia Helena Theodoro, Alvaro Francisco Bosco
OBJECTIVE: To analyze the influence of low-level laser therapy (LLLT) on the bone healing process of autogenous bone block grafts installed in nicotine systemically modified rats. METHODS: Seventy-two rats (Wistar) were randomly assigned into 4 groups (n=18). SS-BG: saline application+bone graft. SS-BG/LLLT: saline application+bone graft+LLLT. NIC-BG: nicotine application+bone graft. NIC-BG/LLLT: nicotine application+bone graft+LLLT. After 30days of application of solutions, all animals received autogenous bone block graft in the jaw, with the donation from the parietal bone's calvarial area...
December 5, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27930925/effects-of-green-tea-and-bisphosphonate-association-on-dental-socket-repair-of-rats
#13
Edson Yoshihiro Mada, Alana Claro Cunha Santos, Angelica Cristina Fonseca, Claudia Cristina Biguetti, Fernando Tozze Alves Neves, Patrícia Pinto Saraiva, Mariza Akemi Matsumoto
OBJECTIVES: To evaluate the effects of green tea intake and zoledronic acid intravenous therapy on teeth socket repair. DESIGN: Sixty male albinus Wistar rats were divided into 4 groups: C-Control, intravenous (IV) 0.9% saline solution (SS), GT-1% green tea in drinking water and IV SS, BP-IV zoledronic acid (BP), and BP+GT-IV BP and 1% green tea. 0.035mg/kg of BP was administered every two weeks. After ten weeks, right upper molars were extracted and the green tea started to be offered for GT and BP+GT...
December 5, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28061390/wettability-and-surface-morphology-of-eroded-dentin-treated-with-chitosan
#14
Mirian Saavedra Ururahy, Fabiana Almeida Curylofo-Zotti, Rodrigo Galo, Lucas Fabricio Bahia Nogueira, Ana Paula Ramos, Silmara Aparecida Milori Corona
OBJECTIVE: To assess the effect of chitosan, at concentrations of 2.5% and 5.0%, on the wettability of the eroded dentin, followed by analysis of surface morphology by SEM. METHODS: 104 bovine dentin slabs were ground, polished and then immersed in 20mL of citric acid (pH=3.2) under continuous stirring for 2h. Specimens were randomly divided according to the dentin substrate: sound and eroded, and then, subdivided into 4 groups (n=10): without rewetting (control), 1% acetic acid, 2...
December 2, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28061389/in-vitro-proliferation-of-periodontal-ligament-like-tissue-on-extracted-teeth
#15
T Iwata, C Mino, T Kawata
OBJECTIVE: Transplantation of autologous teeth is a routine component of orthodontic treatment. The aim of this study was to develop a method for the regeneration of damaged periodontal ligament (PDL) on extracted teeth using a three-dimensional culture system. DESIGN: We used the maxillary first premolars or third molars extracted from patients for orthodontic treatment. The extracted teeth were stained with toluidine blue to measure the residual PDL area. After confirming damage of the periodontal tissue on the root surface of the extracted teeth, we tried to regenerate the periodontal tissue...
November 30, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27836101/tumor-necrosis-factor-alpha-stimulates-cytokine-expression-and-transient-sensitization-of-trigeminal-nociceptive-neurons
#16
Zachary L Durham, Jordan L Hawkins, Paul L Durham
OBJECTIVE: Elevated levels of tumor necrosis factor- alpha (TNF-α) in the capsule of the temporomandibular joint (TMJ) are implicated in the underlying pathology of temporomandibular disorders (TMD). TMD are a group of conditions that result in pain in the TMJ and/or muscles of mastication, and are associated with significant social and economic burdens. The goal of this study was to investigate the effect of elevated TNF-α levels in the TMJ capsule on nocifensive behavioral response to mechanical stimulation of trigeminal neurons and regulation of cytokines within the trigeminal ganglion...
November 4, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27825678/microbiological-and-clinical-assessment-of-the-abutment-and-non-abutment-teeth-of-partial-removable-denture-wearers
#17
Luciana Costa, Cássio do Nascimento, Valéria Oliveira Pagnano de Souza, Vinícius Pedrazzi
OBJECTIVE: The aim of this study was assessing the changes in both clinical and microbiological parameters of healthy individuals after rehabilitation with removable partial denture (RPD). DESIGN: 11 women received unilateral or bilateral free-end saddle RPD in the mandibular arch. Clinical and microbiological parameters of abutment, non-abutment, and antagonist teeth were assessed at baseline (RPD installation) and after 7, 30, 90, and 180days of function. The Checkerboard DNA-DNA hybridization technique was used to identify and quantify up to 43 different microbial species from subgingival biofilm samples...
November 3, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27825679/regulation-of-matrix-metalloproteinase-secretion-by-green-tea-catechins-in-a-three-dimensional-co-culture-model-of-macrophages-and-gingival-fibroblasts
#18
Marie-Pierre Morin, Daniel Grenier
OBJECTIVES: Elevated levels of matrix metalloproteinases (MMPs) have been associated with the active phases of tissue and bone destruction in periodontitis, an inflammatory disease characterized by a significant breakdown of tooth support. In the present study, we used a three-dimensional (3D) co-culture model of macrophages and gingival fibroblasts to investigate the ability of a green tea extract and its major constituent epigallocatechin-3-gallate (EGCG) to regulate the secretion of MMP-3, -8, and -9...
November 2, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27825675/adhesion-of-porphyromonas-gingivalis-and-tannerella-forsythia-to-dentin-and-titanium-with-sandblasted-and-acid-etched-surface-coated-with-serum-and-serum-proteins-an-in-vitro-study
#19
Sigrun Eick, Christian Kindblom, Danuta Mizgalska, Anna Magdoń, Karolina Jurczyk, Anton Sculean, Andreas Stavropoulos
OBJECTIVE: To evaluate the adhesion of selected bacterial strains incl. expression of important virulence factors at dentin and titanium SLA surfaces coated with layers of serum proteins. METHODS: Dentin- and moderately rough SLA titanium-discs were coated overnight with human serum, or IgG, or human serum albumin (HSA). Thereafter, Porphyromonas gingivalis, Tannerella forsythia, or a six-species mixture were added for 4h and 24h. The number of adhered bacteria (colony forming units; CFU) was determined...
November 2, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27825677/altered-autophagy-and-sympathetic-innervation-in-salivary-glands-from-high-fat-diet-mice
#20
Polliane Morais de Carvalho, Maria Beatriz Duarte Gavião, Guy Howard Carpenter
OBJECTIVE: to investigate the effects of a high fat diet (HFD) on salivary glands in vivo, in a mouse model. In particular, whether it will induce the appearance of fat cells in salivary glands, alterations related to autophagy, mTOR pathway and sympathetic innervation. DESIGN: 27 adult female ICR mice were separated in six groups. Three groups fed with (HFD) containing 55% fat, for one, two and three month and another three groups fed with normal diet (2.7% of fat), for the same time periods...
October 29, 2016: Archives of Oral Biology
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