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Archives of Oral Biology

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https://www.readbyqxmd.com/read/28898790/cytotoxicity-and-potential-anti-inflammatory-activity-of-velutin-on-raw-264-7-cell-line-differentiation-implications-in-periodontal-bone-loss
#1
Carlos Brito, Alexander Stavroullakis, Tatiane Oliveira, Anuradha Prakki
OBJECTIVES: Hypoxia-inducible factor-1α (HIF-1α) has been implicated in periodontal tissue inflammation and possibly in osteoclast differentiation, while polyphenols are known to be anti-inflammatory natural compounds that are capable of regulating the NF-κB protein complex pathway. The objective of this study was to investigate cytotoxicity and HIF-1α expression through the NF-κB pathway by polyphenol velutin (Euterpe oleracea Mart.), found in the pulp of acai fruit, during inflammatory RAW 264...
September 2, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28892665/difference-in-glycogen-metabolism-glycogen-synthesis-and-glycolysis-between-normal-and-dysplastic-malignant-oral-epithelium
#2
Hitoshi Aizawa, Shin-Ichi Yamada, Tiepeng Xiao, Tetsu Shimane, Kiyonori Hayashi, Fangfang Qi, Hirokazu Tanaka, Hiroshi Kurita
BACKGROUND: The purpose of this study was to investigate a difference in glycogen metabolism (glycogen synthesis and glycolysis) between the iodine stained (normal non-keartinized) and the unstained (dysplasctic/malignant) oral epithelium. METHODS: Twenty-one frozen tissue samples of iodine-stained and unstained mucosal tissue were obtained from 21 OSCC patients. Serial frozen sections were cut and examined with the hematoxylin-eosin and periodic acid-Schiff methods and immunohistochemical (IHC) staining for Ki67, P53, molecules associated with glycogenesis (i...
September 2, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28869827/effects-of-dexamethasone-and-nimesulide-on-bisphosphonate-related-osteonecrosis-of-the-jaw-an-experimental-study
#3
Camila Carvalho de Oliveira, Paulo Goberlânio de Barros Silva, Antonio Ernando Carlos Ferreira, Romélia Pinheiro Gonçalves, Fabrício Bitu de Sousa, Mário Rogério Lima Mota, Ana Paula Negreiros Nunes Alves
OBJECTIVE: To evaluate the effects of dexamethasone (DEX) and nimesulide (NIM) on Bisphosphonate-related Osteonecrosis of the Jaw (BRONJ) in rats. DESIGN: BRONJ was induced by zoledronic acid (ZA) infusion (0.2mg/kg) in Wistar rats (n=8), followed by extraction of the left lower first molar (BRONJ groups). Control groups (n=40) received saline (IV). For eight weeks, DEX (0.04, 0.4, 4mg/kg) or saline (SAL) were administered by gavage 24h before each infusion of ZA or saline (IV), or NIM (10...
August 30, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28865353/mechanisms-involved-in-facial-heat-hyperalgesia-induced-by-endothelin-1-in-female-rats
#4
Rafael Fernandes de Souza, Luana Lechenakoski de Oliveira, Carina Fernanda Mattedi Nones, Renata Cristiane Dos Reis, Erika Ivanna Araya, Caroline Machado Kopruszinski, Giles Alexander Rae, Juliana Geremias Chichorro
OBJECTIVE: Pronociceptive responses to endothelins in the trigeminal system seem to be mediated by ETA and ETB receptors, which have been shown to be expressed in neurons of the trigeminal ganglion of humans and rats. The present study aimed to evaluate the ability of endothelin-1 (ET-1) to induce facial heat hyperalgesia in female rats, the contribution of ETA and ETB receptors to this response, as well as the mechanisms underlying heat hyperalgesia induced by ET-1. DESIGN: ET-1 (100pmol/50μL) was injected into the upper lip and heat hyperalgesia was evaluated for up to 6h...
August 30, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28886585/human-papillomavirus-infection-in-oral-potentially-malignant-disorders-and-cancer
#5
REVIEW
Xun Chen, Yu Zhao
Human papillomavirus (HPV) infects keratinocytes in the mucosa or skin, and persistent infection with HPV may lead to premalignant lesions and invasive cancer, especially cervical cancer. It has also been hypothesized that HPV infection is an etiological factor of oral squamous cell carcinoma and oral precancerous disorders such as lichen planus, leukoplakia, and erythroplakia. A high percentage of HPV in oral lesions supports the possible viral contribution, but an association of HPV infection with these lesions remains to be established...
August 26, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28866437/adiponectin-prevents-orthodontic-tooth-movement-in-rats
#6
Sigrid Haugen, Kristin Matre Aasarød, Astrid Kamilla Stunes, Mats Peder Mosti, Tanya Franzen, Vaska Vandevska-Radunovic, Unni Syversen, Janne Elin Reseland
OBJECTIVE: The objective was to examine the effects of repetitive local administration of adiponectin on experimental tooth movement in rats. MATERIALS AND METHODS: The maxillary right first molar of male Wistar rats (n=24) was moved mesially for 14days, with local adiponectin injections (0.2 or 2μg) every third day. Micro-computed tomography was performed at days 0, 6 and 14 and molar movement, bone density and bone volume fraction were calculated from the scans...
August 26, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28886584/human-pulpal-blood-flow-in-different-root-formation-stages-measured-with-transmitted-light-plethysmography
#7
Khongorzul Ganbold, Satoko Kakino, Hideharu Ikeda, Michiyo Miyashin
OBJECTIVES: To determine the pulp vitality after traumatic injury, dentists often use pulp sensitivity tests, which can be ambiguous in young permanent teeth with incomplete root formation. Transmitted-light plethysmography (TLP) is a non-invasive objective method that uses a 525-nm LED to detect blood volume change in the pulp. The present study aimed (1) to investigate pulpal blood flow with TLP and optical characteristics in healthy permanent maxillary incisors in different root formation stages, and (2) to assess the influences of body growth of the children and tooth color on the TLP amplitude...
August 25, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28866438/observations-on-fluid-flow-from-exposed-dentine-in-primary-teeth-an-in-vitro-study
#8
Monticha Rangcharoen, Varisara Sirimaharaj, Sitthichai Wanachantararak, Noppakun Vongsavan, Bruce Matthews
OBJECTIVE: To investigate fluid flow through dentine in primary teeth in vitro using the replica technique, and to compare the results with those obtained from permanent dentine. DESIGN: The experiments were carried out on 22 extracted, mandibular, primary, incisor teeth. The incisal edge was removed to 1mm below the dentino-enamel junction and half the exposed surface etched with phosphoric acid. The exposed dentine was blotted dry and the pressure in the pulp cavity held at 0, 15, 30 or 45cm H2O above atmospheric for 30s...
August 25, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28865352/is-it-really-penetration-locomotion-of-devitalized-enterococcus-faecalis-cells-within-dentinal-tubules-of-bovine-teeth
#9
Jasmin Kirsch, Sabine Basche, Jörg Neunzehn, Maria Dede, Martin Dannemann, Christian Hannig, Marie-Theres Weber
OBJECTIVE: The aim of the present study was to evaluate the penetration characteristics of devitalized and vital E. faecalis cells into root dentinal tubules. DESIGN: Thirteen root canals were incubated with devitalized (4days, 7days, 14days, 28days) and vital (28days) E. faecalis strains (streptomycin-resistant strains) after root canal enlargement (size 80, taper 0.02) with 3 % NaOCl solution. The smear layer was intentionally removed with 20 % EDTA before inoculation...
August 25, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28843745/structural-mechanical-and-chemical-evaluation-of-molar-incisor-hypomineralization-affected-enamel-a-systematic-review
#10
REVIEW
Karim Elhennawy, David John Manton, Felicity Crombie, Paul Zaslansky, Ralf J Radlanski, Paul-Georg Jost-Brinkmann, Falk Schwendicke
OBJECTIVES: To systematically assess and contrast reported differences in microstructure, mineral density, mechanical and chemical properties between molar-incisor-hypomineralization-affected (MIH) enamel and unaffected enamel. METHODS: Studies on extracted human teeth, clinically diagnosed with MIH, reporting on the microstructure, mechanical properties or the chemical composition and comparing them to unaffected enamel were reviewed. Electronic databases (PubMed, Embase and Google Scholar) were screened; hand searches and cross-referencing were also performed...
August 19, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28841473/-metabolism-of-odontoblast-like-cells-submitted-to-transdentinal-irradiation-with-blue-and-red-led
#11
Leopoldina de Fátima Dantas de Almeida, Fernanda Gonçalves Basso, Ana Paula Silveira Turrioni, Carlos Alberto de-Souza-Costa, Josimeri Hebling
OBJECTIVES: The present study evaluated the trans-dentinal effect of light emitting diodes (LEDs) irradiation on the metabolism of odontoblast-like cells. METHODS: Seventy-two dentin discs (0.2mm thick) were obtained from human molar teeth. MDPC-23 cells (20,000 cells/disc) were seeded on the pulpal side of the discs using DMEM, supplemented with 10% fetal bovine serum (FBS). After 12h, the culture medium was replaced with DMEM containing 0.5% FBS. After additional 12h, blue (455±10nm) or red (630±10nm) LEDs were used at irradiances of 80 and 40mW/cm(2), respectively, to irradiate the occlusal side of the discs...
August 17, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28858630/quantitative-assessment-of-salivary-oral-bacteria-according-to-the-severity-of-dental-caries-in-childhood
#12
Natália H Colombo, Paula F Kreling, Laís F F Ribas, Jesse A Pereira, Christine A Kressirer, Marlise I Klein, Anne C R Tanner, Cristiane Duque
This study aimed to assess differences in selected oral bacteria in children according to the severity of dental caries. One hundred and thirty-six children, 36-60 months old were divided into three groups according to caries status: caries-free (CF) (n=47), early childhood caries (ECC) (n=40) and severe-early childhood caries (S-ECC) (n=49). Saliva was collected for detection and quantification of selected oral streptococci, Actinomyces naeslundii, Lactobacillus spp., Bifidobacterium spp., and Scardovia wiggsiae by quantitative-polymerase chain reaction...
August 16, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28841474/relatively-low-invasive-capacity-of-porphyromonas-gingivalis-strains-into-human-gingival-fibroblasts-in-vitro
#13
Ju Young Jang, Keum Jin Baek, Youngnim Choi, Suk Ji
OBJECTIVE: Bacterial invasion into host cells is a common strategy to escape the host immune system. Gingival fibroblasts (GFs) are the most predominant non-phagocytic cell type in gingival connective tissue. Therefore, invasion into GFs was thought to be the first strategy for the survival of Porphyromonas gingivalis. The present study compared the invasive ability of P. gingivalis into GFs with those of other red-complex and relatively less pathogenic bacterial strains, especially Fusobacterium nucleatum...
August 16, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28822800/amelogenin-induces-m2-macrophage-polarisation-via-pge2-camp-signalling-pathway
#14
Kensuke Yamamichi, Takao Fukuda, Terukazu Sanui, Kyosuke Toyoda, Urara Tanaka, Yuki Nakao, Karen Yotsumoto, Hiroaki Yamato, Takaharu Taketomi, Takeshi Uchiumi, Fusanori Nishimura
OBJECTIVES: Amelogenin, the major component of the enamel matrix derivative (EMD), has been suggested as a bioactive candidate for periodontal regeneration. Apart from producing a regenerative effect on periodontal tissues, amelogenin has also been reported to have an anti-inflammatory effect. However, the precise molecular mechanisms underlying these effects remain unclear. In the present study, we examined the immunomodulatory effects of amelogenin on macrophages. DESIGN: Human phorbol 12-myristate 13-acetate (PMA)-differentiated U937 macrophages and CD14+ peripheral blood-derived monocytes (PBMC)-derived macrophages were stimulated with recombinant amelogenin (rM180)...
August 12, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28829993/effect-of-different-dental-burs-for-experimental-induction-of-pulpitis-in-mice
#15
Xilin Shi, Zhengmao Li, Ying He, Qianzhou Jiang, Xuechao Yang
OBJECTIVE: To evaluate the effect of using different dental burs on the development of pulpal inflammation after pulpal exposure in mice. DESIGN: Eighty-eight C57BL/6 mice were randomly assigned to group A (n=40), group B (n=40) and control group (n=8). The pulps of the maxillary first molars were occlusally exposed using ¼ round burs and polishing burs in group A and B respectively. Animals were sacrificed randomly at 0h, 4h, 8h, 12h and 24h after pulpal exposure...
August 11, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28822799/effect-of-application-sequence-of-fluoride-and-cpp-acp-on-remineralization-of-white-spot-lesions-in-primary-teeth-an-in-vitro-study
#16
Ola B Al-Batayneh, Reem A Jbarat, Susan N Al-Khateeb
OBJECTIVE: To explore how application sequence of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride influences remineralization of enamel white spot lesions (WSL) in primary teeth. DESIGN: In this in-vitro study, artificial WSLs were created in 130 primary teeth. Teeth were divided into 4 groups (n=27) and a control group (n=22) and exposed to one of the following remineralization regimens for 10 weeks: Group-1; 500ppm fluoride dentifrice; Group-2; 10% w/v CPP-ACP; Group-3; fluoride applied first, then CPP-ACP; Group-4; CPP-ACP applied first, then fluoride, and Group-5 was control...
August 10, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28802191/a-novel-method-for-sex-estimation-using-3d-computed-tomography-models-of-tooth-roots-a-volumetric-analysis
#17
Seyedeh M Kazzazi, Elena F Kranioti
Advances in technologies such as computed tomography (CT) scanning have allowed for further examination of dental sexual dimorphism in modern and archaeological populations.
August 4, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28810187/histochemical-and-immunohistochemical-examination-of-odontoblasts-petroblasts-in-petrodentine-formation-of-lungfish
#18
Shunya Oka, Ichiro Sasagawa, Mikio Ishiyama
OBJECTIVE: Petrodentine, the core of the lungfish tooth plate, is a well-mineralized tissue similar to mammalian enamel and analogous to enameloid in fish teeth. Petrodentine is formed solely by petroblasts, which are specialized odontoblasts, whereas enameloid is a composite tissue produced by both odontoblasts and dental epithelial cells. To clarify the details of petrodentine formation, petroblasts were investigated using histochemical and immunohistochemical techniques. METHODS: Extant lungfish (Lepidosiren paradoxa) were used in this study...
August 3, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28818706/diabetes-increases-interleukin-17-levels-in-periapical-hepatic-and-renal-tissues-in-rats
#19
Mariane Maffei Azuma, João Eduardo Gomes-Filho, Annelise Katrine Carrara Prieto, Renata Oliveira Samuel, Valéria Marçal Felix de Lima, Dóris Hissako Sumida, Edilson Ervolino, Luciano Tavares Angelo Cintra
OBJECTIVES: This study aimed to evaluate the association between endodontic infection and diabetes on interleukin-17 levels in periapical, hepatic, and renal tissues of rats. DESIGN: Forty male rats were divided into groups: normoglycemic rats (N), normoglycemic rats with apical periodontitis (N-AP), rats with experimental diabetes (ED), and rats with experimental diabetes and apical periodontitis (ED-AP). Diabetes was induced by intravenous streptozotocin injection, and blood sugar levels were monitored to confirm disease development...
August 2, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28783551/extracellular-hsp72-induces-proinflammatory-cytokines-in-human-periodontal-ligament-fibroblast-cells-through-the-tlr4-nf%C3%AE%C2%BAb-pathway-in-vitro
#20
Chihiro Arai, Yoshiaki Nomura, Masazumi Matsuzawa, Nobuhiro Hanada, Yoshiki Nakamura
OBJECTIVE: The aim of the present study was to examine the effect of extracellular heat shock protein (HSP) 72 on human periodontal ligament fibroblast cells (hPDLFs) in vitro. DESIGN: hPDLFs were stimulated by recombinant human HSP72 (rhHSP72). TAK-242 was used to inhibit toll-like receptor 4 (TLR4) activity. Interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF)-α mRNA levels were analyzed by real-time PCR and protein levels were analyzed by enzyme-linked immunosorbent assay...
July 30, 2017: Archives of Oral Biology
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