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Laboratory Animals

Natalie Burden, John Kendrick, Lindsay Knight, Victoria McGregor, Helen Murphy, Malcolm Punler, Hans van Wijk
Bile duct cannulation (BDC) studies are usually carried out in the rat to support the absorption, distribution, metabolism and excretion profiling of novel agrochemicals and pharmaceuticals. The different aspects of these studies (e.g. surgical preparation, dosing and collection of bile) can be intricate and/or technically complex. The animals are often kept singly housed for the duration of the studies following surgical implantation of the cannulas. The generation of insufficient data to meet the study objectives, for example due to failure in cannula patency, can result in the need to repeat these studies...
October 2017: Laboratory Animals
Henri G M J Bertrand, Svenja Springer, Wesley Burnside, Charlotte Sandersen, Paul A Flecknell
Volatile agents for anaesthesia are widely used for anaesthetizing laboratory primates, and isoflurane is one of the most frequently used agents. Sevoflurane has been shown to offer a more rapid recovery than isoflurane in a number of species, but no comparisons have been made in non-human primates. This study compared the recovery characteristics of isoflurane and sevoflurane in rhesus macaques undergoing experimental neurosurgery. Twelve primates (7 males and 5 females) were randomly allocated to the treatment groups...
October 2017: Laboratory Animals
Vijay Pal Singh
No abstract text is available yet for this article.
October 2017: Laboratory Animals
Yong Cheng, Lucas D Liepert, Justin Waller, W Matthew Leevy, Jeff S Schorey
Intratracheal injection is a traditional technique used in small animal studies of highly contagious airborne pathogens such as Mycobacterium tuberculosis. However, current techniques of intratracheal injection generally involve procedures that pose a risk of incident injury and infection for researchers, and may also cause collateral damage to experimental animals during the installation process. Here we describe an intratracheal injection method that was enabled by a three dimensional printing of a custom platform...
October 2017: Laboratory Animals
Elin Manell, Marianne Jensen-Waern, Patricia Hedenqvist
Pigs are commonly used in diabetes research due to their many physiological similarities to humans. They are especially useful in imaging procedures because of their large size. However, to achieve imaging procedures the pig must lie completely still, and thus needs to be anaesthetized. Most anaesthetic drugs used in laboratory animals affect carbohydrate metabolism by the inhibition of insulin release. The aim of this pilot study was primarily to develop an anaesthetic protocol for pigs that did not have an effect on blood glucose levels throughout the 3 h of anaesthesia; and secondly, to evaluate the most promising protocol in combination with an oral glucose tolerance test (OGTT)...
October 2017: Laboratory Animals
Julie L Eiseman, Michael Sciullo, Hong Wang, Jan H Beumer, Charles C Horn
Several cancer chemotherapies cause nausea and vomiting, which can be dose-limiting. Musk shrews are used as preclinical models for chemotherapy-induced emesis and for antiemetic effectiveness. Unlike rats and mice, shrews possess a vomiting reflex and demonstrate an emetic profile similar to humans, including acute and delayed phases. As with most animals, dosing of shrews is based on body weight, while translation of such doses to clinically equivalent exposure requires doses based on body surface area. In the current study body surface area in musk shrews was directly assessed to determine the Meeh constant (Km) conversion factor (female = 9...
October 2017: Laboratory Animals
Aurelie Anic Thomas, Johann Detilleux, Charlotte Friederike Sandersen, Paul Andrew Flecknell
The intrathecal (IT) route of administration represents a means to reduce the dose of morphine administered for analgesia, potentially minimizing interactions between opioid effects and experimental outcomes. Perceived technical difficulty, and previously described invasive methods, may limit its use. This report describes a minimally invasive technique for IT administration of morphine by direct transcutaneous lumbosacral puncture in rats; and assesses antinociceptive properties of morphine in anaesthetized rats...
October 2017: Laboratory Animals
David Tibor Lauber, András Fülöp, Tibor Kovács, Krisztián Szigeti, Domokos Máthé, Attila Szijártó
In recent decades, imaging devices have become indispensable tools in the basic sciences, in preclinical research and in modern drug development. The rapidly evolving high-resolution in vivo imaging technologies provide a unique opportunity for studying biological processes of living organisms in real time on a molecular level. State of the art small-animal imaging modalities provide non-invasive images rich in quantitative anatomical and functional information, which renders longitudinal studies possible allowing precise monitoring of disease progression and response to therapy in models of different diseases...
October 2017: Laboratory Animals
Paul K J D de Jonge, Marije Sloff, Heinz P Janke, Barbara B M Kortmann, Robert P E de Gier, Paul J Geutjes, Egbert Oosterwijk, Wout F J Feitz
It is common to test medical devices in large animal studies that are or could also be used in humans. In this short report we describe the use of a ureteral J-stent for the evaluation of biodegradable tubular constructs for tissue reconstruction, and the regeneration of ureters in Saanen goats. Similarly to a previous study in pigs, the ureteral J-stent was blindly inserted until some resistance was met. During evaluation of the goats after three months, perforation of the renal cortex by the stent was observed in four out of seven animals...
October 2017: Laboratory Animals
Nuno Franco
No abstract text is available yet for this article.
August 2017: Laboratory Animals
Anton M Pluschke, Gregory S Simmons, Helen L Keates, Ranald D A Cameron, Dagong Zhang, John D Wright, Barbara A Williams
Jugular catheterization is a common procedure used under experimental conditions. However, there is considerable variation in the reported techniques, particularly for grower pigs (>40 kg and <60 kg) when larger volumes of blood per sample (>10 mL) are required. This paper provides a complete methodology including the use of current equipment and anaesthetic regimen for grower pigs. This surgical jugular catheterization method was carried out in 30 large white grower pigs. Firstly, the pigs were habituated to human handling for at least two weeks prior to surgery...
August 2017: Laboratory Animals
Jose Orellana, Eleanor Weir
No abstract text is available yet for this article.
June 2017: Laboratory Animals
Paulin Jirkof, Beat M Riederer, Arieh Bomzon
No abstract text is available yet for this article.
June 2017: Laboratory Animals
Grupo de Trabajo de la Secal
No abstract text is available yet for this article.
June 2017: Laboratory Animals
Beat M Riederer, Arieh Bomzon, Paulin Jirkof
Good communication is important for the dissemination of research results. Here, we summarize the advice that was given to authors at the LA seminar on scientific writing at the FELASA Congress 2016 in Brussels, Belgium on 13-16 June 2016, with the aim of improving the quality of submitted papers and of avoiding common mistakes in scientific reports. See .
June 2017: Laboratory Animals
Ekaterina A Litvinova, Elena N Kozhevnikova, Ksenia M Achasova, Galina V Kontsevaya, Mikhail P Moshkin
Infections with Helicobacter spp. are known to have serious effects on rodent health, especially in immunocompromised animals. In this study three approaches were used to eradicate Helicobacter spp. infection in mice with a deficiency in intestinal proteoglycan (mucin2), namely triple oral antibiotic therapy (amoxicillin, clarithromycin and metronidazole), cross-fostering of neonatal pups by surrogate mothers negative for Helicobacter spp., and in vitro fertilization (IVF) with embryo transfer into Helicobacter-free mothers...
June 2017: Laboratory Animals
Sara Hestehave, Gordon Munro, Tina Brønnum Pedersen, Klas S P Abelson
Researchers performing experiments on animals should always strive towards the refinement of experiments, minimization of stress and provision of better animal welfare. An adequate analgesic strategy is important to improve post-operative recovery and welfare in laboratory rats and mice. In addition, it is desirable to provide post-operative analgesia using methods that are minimally invasive and stressful. This study investigated the antinociceptive effects of orally administered buprenorphine ingested in Nutella® in comparison with subcutaneous buprenorphine administration...
June 2017: Laboratory Animals
Louise Saul, Dario Besusso, Richard J Mellanby
The pathogenesis of human autoimmune disorders is incompletely understood. This has led to the development of numerous murine models in which the pathogenesis of autoimmunity can be probed and the efficacy of novel therapies can be tested. One of the most widely-used murine models of autoimmunity is experimental autoimmune encephalomyelitis (EAE). To induce autoimmune pathology, mice are often immunized with an autoantigen alongside an adjuvant, typically complete Freund's adjuvant (CFA). Unfortunately, CFA causes significant inflammation at the site of administration...
June 2017: Laboratory Animals
Vivi Flou Hjorth Jensen, Anne-Marie Mølck, Martin Mårtensson, Mette Aagaard Strid, Melissa Chapman, Jens Lykkesfeldt, Ingrid Brück Bøgh
Group housing is considered to be important for rats, which are highly sociable animals. Single housing may impact behaviour and levels of circulating stress hormones. Rats are typically used in the toxicological evaluation of insulin analogues. Human insulin (HI) is frequently used as a reference compound in these studies, and a comparator model of persistent exposure by HI infusion from external pumps has recently been developed to support toxicological evaluation of long-acting insulin analogues. However, this model requires single housing of the animals...
June 2017: Laboratory Animals
Julia Zorn, Bärbel Ritter, Manuel Miller, Monika Kraus, Emily Northrup, Markus Brielmeier
One limitation to housing rodents in individually ventilated cages (IVCs) is the ineffectiveness of traditional health monitoring programs that test soiled bedding sentinels every quarter. Aerogen transmission does not occur with this method. Moreover, the transmission of numerous pathogens in bedding is uncertain, and sentinel susceptibility to various pathogens varies. A novel method using particle collection from samples of exhaust air was developed in this study which was also systematically compared with routine health monitoring using soiled bedding sentinels...
June 2017: Laboratory Animals
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