Read by QxMD icon Read

Laboratory Animals

Penny S Reynolds, Jacquelyn McCarter, Christopher Sweeney, Bassem M Mohammed, Donald F Brophy, Bernard Fisher, Erika J Martin, Ramesh Natarajan
Poor quality data in preclinical trials can result from inconsistent and unstandardized experimental processes. Unpredictable pre-intervention variability generates unreliable data, biases outcomes and results in needless waste of animals and resources. We applied Define-Measure-Analyse-Improve-Control (DMAIC) quality improvement processes to pilot development of a swine model of trauma, haemorrhagic shock and coagulopathy. The goal was to reduce variability through protocol standardization and error reduction...
October 9, 2018: Laboratory Animals
Tina Nitezki, Burkhard Kleuser, Stephanie Krämer
This case report addresses the problem of underreporting negative results and adverse side effects in animal testing. We present our findings regarding a hyperphagic mouse model associated with unforeseen high mortality. The results outline the necessity of reporting detailed information in the literature to avoid duplication. Obese mouse models are essential in the study of obesity, metabolic syndrome and diabetes mellitus. An experimental model of obesity can be induced by the administration of gold thioglucose (GTG)...
October 5, 2018: Laboratory Animals
Jamie Ahloy-Dallaire, Jon D Klein, Jerry K Davis, Joseph P Garner
Routine health assessment of laboratory rodents can be improved using automated home cage monitoring. Continuous, non-stressful, objective assessment of rodents unaware that they are being watched, including during their active dark period, reveals behavioural and physiological changes otherwise invisible to human caretakers. We developed an automated feeder that tracks feed intake, body weight, and physical appearance of individual radio frequency identification-tagged mice in social home cages. Here, we experimentally induce illness via lipopolysaccharide challenge and show that this automated tracking apparatus reveals sickness behaviour (reduced food intake) as early as 2-4 hours after lipopolysaccharide injection, whereas human observers conducting routine health checks fail to detect a significant difference between sick mice and saline-injected controls...
October 4, 2018: Laboratory Animals
Helen Gray, Alexander Thiele, Candy Rowe
Rhesus macaques ( Macaca mulatta) used in behavioural neuroscience are often required to complete cognitively complex tasks, for which a high level of motivation is essential. To induce motivation, researchers may implement fluid-restriction protocols, whereby freely available water is limited, such that fluid can be used as a reward in the laboratory. A variety of different rewards and schedules are used, but there exists a lack of data assessing their effectiveness. In this study, we aimed to quantify fluid preference in rhesus macaques and to use these preferences to compare the motivational quality of different reward schedules: the monkey's previous reward (i...
October 3, 2018: Laboratory Animals
Elina Akalestou, Laurent Genser, Francesco Villa, Ioannis Christakis, Shilpa Chokshi, Roger Williams, Francesco Rubino
Gastric bypass surgery, an operation that restricts the stomach and bypasses the duodenum and part of the jejunum, results in major improvement or remission of type 2 diabetes. Duodenual-jejunal bypass was developed by one of the authors (FR) as an experimental, stomach-sparing variant of gastric bypass surgery to investigate weight-independent mechanisms of surgical control of diabetes. Duodenual-jejunal bypass has been shown to improve various aspects of glucose homeostasis in rodents and in humans, thus providing an experimental model for investigating mechanisms of action of surgery and elusive aspects of gastrointestinal physiology...
September 18, 2018: Laboratory Animals
Agustina Resasco, Ana C Carranza Martin, Miguel A Ayala, Silvina L Diaz, Cecilia Carbone
We have developed a new method for the measurement of subcutaneous tumour volume which consists in taking photographs of mice in their home cages, to refine the standard method of measurement with calipers. We consider this new method to be non-aversive, as it may be more compatible with mice behavioural preferences and, therefore, improve their welfare. Photographs are captured when mice voluntarily go into an acrylic tube containing graph paper that is later used as a scale. Tumour volumes measured with the caliper and the non-aversive photographic method were compared to those obtained by water displacement volume and weight...
August 21, 2018: Laboratory Animals
Dallas W Henderson, Brian C Small
The turquoise killifish Nothobranchius furzeri is an increasingly popular model species for comparative vertebrate research, and the basic physiology including responses to stressful stimuli are of primary interest. We exposed adult killifish to a single or repeated periods of acute confinement followed by analysis of tissue cortisol and plasma cortisol concentrations. Individuals were also sampled for messenger RNA (mRNA) expression of corticotropin-releasing hormone ( CRH), mineralocorticoid receptor ( MR), and glucocorticoid receptor ( GR) in the brain to examine the effects of repeated stress events on constitutive expression of these important stress axis components...
August 20, 2018: Laboratory Animals
Randi Lundberg
Mouse colonized with human fecal microbiota is an interesting model concept with pros and cons like any other model system. The concept provides an ecologically relevant context to study food component and drug metabolism, and is an invaluable tool for phenotype transfer studies to prove the role of the gut microbiota in health and disease. The major drawbacks are the difficulties with transferring certain components of the human microbiota to the recipient mice, and immunological abnormalities observed in these mice...
August 8, 2018: Laboratory Animals
Marlene Resch, Tania Neels, Alexander Tichy, Rupert Palme, Thomas Rülicke
Intravenous (IV) administration in mice is predominantly performed via the lateral tail veins. The technique requires adequate training before it can be used safely and routinely. A novel anaesthesia induction chamber has been developed to simplify the treatment and to facilitate IV injection in mice, particularly for untrained personnel. We have assessed the benefits of the chamber in refining IV injection in isoflurane-anaesthetized mice in direct comparison with the common restrainer method on conscious animals...
August 8, 2018: Laboratory Animals
Louise K Jensen, Nicole L Henriksen, Henrik E Jensen
During the last 10 years the number of porcine models for human bacterial infectious diseases has increased. In the future, this tendency is expected to continue and, therefore, the aim of the present review is to describe guidelines for the development and reporting of these models. The guidelines are based on a review of 122 publications of porcine models for different bacterial infectious diseases in humans. The review demonstrates a substantial lack of information in most papers which hampers reproducibility and continuation of the work that was established in the models...
August 8, 2018: Laboratory Animals
Hedwig Stegmann, Tobias Bäuerle, Katharina Kienle, Sven Dittrich, Muhannad Alkassar
Preclinical cardiovascular research is the foundation of our understanding and broad knowledge of heart function and cardiovascular disease. Reliable cardiac imaging modalities are the basis for applicable results. Four-dimensional cardiac magnetic resonance (4D CMR) has been set as the gold standard for in-vivo assessment of ventricular function in rodents. However, technical improvements in echocardiography now allow us to image the whole heart, which makes four-dimensional echocardiography (4DE) a possible alternative to 4D CMR...
August 6, 2018: Laboratory Animals
Vera Baumans, Pim Rooymans, Jan-Bas Prins
No abstract text is available yet for this article.
August 2, 2018: Laboratory Animals
Yanzi Zhang, Bo Cui, Chunyu Gong, Yidan Tang, Jianxiong Zhou, Yi He, Jin Liu, Jing Yang
It is important to develop a feasible animal model of regional anesthesia other than sciatic nerve blockade for pharmacokinetic investigations of new local anesthetics or analgesia in upper extremity surgery. Herein, we explored a nerve stimulator (NS)-guided brachial plexus block (BPB) in a rat model. The anatomy of the brachial plexus in rats was delineated in cadavers, and various BPBs were examined. The puncture point was located 0.5-1.0 cm below the lateral one-third of the clavicle. The efficacy and safety of the NS-guided BPB were evaluated using an injection of 2% lidocaine or 0...
July 26, 2018: Laboratory Animals
Alexandra Kovalčíková, Marianna Gyurászová, Roman Gardlík, Martin Boriš, Peter Celec, Ľubomíra Tóthová
Representative urine collection that respects the standards of animal welfare is still an issue in experimental nephrology. The commonly used metabolic cages induce stress in rodents. In mice, the volume of collected urine is sometimes insufficient for further analysis. The aim of this experiment was to analyse the effects of time of day, temperature and 2%, 5% or 10% sucrose solutions on diuresis, weight change and liquid intake of adult mice placed in metabolic cages for urine collection. Mice were placed in metabolic cages for 12 h during the day or night at standard ambient (22℃) and thermoneutral (28℃) temperatures...
July 25, 2018: Laboratory Animals
Marcel Gyger, Manuel Berdoy, Ismene Dontas, Martine Kolf-Clauw, Ana Isabel Santos, Mats Sjöquist
This document describes how the Federation of European Laboratory Animal Science Associations (FELASA) accreditation addresses both the Directive 2010/63/EU and the related European Commission guidance document. The four EU Functions and beyond: FELASA accredits courses that fulfil the requirements of Functions A, B, C and D as defined by EU Directive, Article 23, as well as for designated veterinarians and specialists in laboratory animal science. Modularity and mobility: Cohesive courses for Functions and for very specific topics are accredited, but flexibility and mobility are possible: a researcher can start his/her training with one FELASA accredited course and complete other modules with another...
July 24, 2018: Laboratory Animals
Jean-Philippe Mocho
No abstract text is available yet for this article.
October 2018: Laboratory Animals
Beat M Riederer, Gavin E Jarvis, Paulin Jirkof
No abstract text is available yet for this article.
October 2018: Laboratory Animals
Janet Rodgers
No abstract text is available yet for this article.
October 2018: Laboratory Animals
(no author information available yet)
No abstract text is available yet for this article.
August 2018: Laboratory Animals
(no author information available yet)
No abstract text is available yet for this article.
August 2018: Laboratory Animals
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"