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Bratislavské Lekárske Listy

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https://www.readbyqxmd.com/read/29322811/establishment-of-reverse-transcription-polymerase-chain-reaction-assay-for-simultaneous-detection-of-human-parainfluenza-virus-types-1-to-4-in-children-with-influenza-like-illnesses
#1
M GhelichKhan, S M Hosseini, B Khansarinejad, F Fotouhi
OBJECTIVES: The aim of this study was to develop an in-house multiplex reverse transcription polymerase chain reaction (mRT-PCR), which can recognize HPIV1-4 in clinical samples. BACKGROUND: Human parainfluenza virus (HPIV) is one of the major causes of viral respiratory infections and can affect people at any age, especially infants and young children. METHODS: Four sets of specific primers targeting conserved areas of hemagglutinin-neuraminidase (HN) genes of HPIV1-4, were designed and tested with type-related plasmid controls...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29322810/therapeutic-hypothermia-and-myocardium-in-perinatal-asphyxia-a-microvolt-t-wave-alternans-and-doppler-echocardiography-study
#2
D Karpuz, Y Celik, D Giray, B Tasdelen, O Hallioglu
OBJECTIVES: This is the first study evaluating the predictive value of myocardial performance on arrhythmia and mortality via tissue-Doppler and microvolt T-wave alternans in infants with hypoxic-ischemic encephalopathy treated with therapeutic hypothermia-rewarming. METHODS: The study included 23 term newborns having criteria for hypoxic-ischemic encephalopathy, and 12 controls. Tissue-Doppler imaging and T-wave alternans were performed in the first six hours after birth in patients from both groups and after hypothermia-rewarming treatment on the fifth day...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29322809/effects-of-2100-mhz-radio-frequency-radiation-on-ductus-epididymis-tissue-in-rats
#3
C Erdemli, S Omeroglu, B Sirav, M Colbay, N Seyhan, S Ozkan, I Yetkin
PURPOSE: The use of mobile phones is widespread since the beginning of 1990s. A great debate exists about the possible damage that the Radio Frequency - RF radiation from mobile phones exerts on different organs. The objective of this study was to investigate the possible histopathological effects of 2100 MHz RF radiation on rat ductus epididymis tissue using a light microscopy and immunohistochemical method after one or two month exposure. MATERIALS AND METHODS: The study was performed on 36 adult Wistar albino rats...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29322808/effects-of-epidermal-growth-factor-on-reduction-of-the-formation-of-thrombus-and-vessel-wall-healing-in-an-experimental-rat-model
#4
N Yumusak, U Yavuz, B Sarikaya, G Yucel
OBJECTIVES: The aim of the current study was to investigate the synergistic effects of epidermal growth factor (EGF) and Enoxaparin in thrombus resolution. METHOD: Forty rats were divided into five groups (n = 8/group). Thrombosis was induced in all groups except the Sham group. Group 1: Sham; Group 2: Phosphate buffered saline; Group 3: Enoxaparin; Group 4: EGF; Group 5: EGF+Enoxaparin. The treatments were applied 2 hours preoperatively, then postoperatively at 48 hours...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29322807/chronic-postsurgical-pain-in-mixed-surgical-population-does-an-acute-pain-service-make-a-difference
#5
V Kubricht, P Sevcik
OBJECTIVES: To investigate the influence of an Acute Pain Service (APS) on the incidence of chronic postsurgical pain (CPSP). To assess the acute pain intensity as a risk factor for CPSP. The impact of an APS on the incidence of CPSP has not yet been studied. METHODS: Retrospective questionnaire given to randomized cohorts study, performed in two hospitals - Hospital A with an APS and Hospital B without such service. 1444 patients underwent eight different surgical procedures in both hospitals within one year, 175 patients from each hospital were randomized...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29322806/periodontitis-aggravates-kidney-damage-in-obese-mice-by-mmp2-regulation
#6
P Chen, D Y Xuan, J C Zhang
OBJECTIVE:   To investigate the effect of periodontitis on the development of kidney damage in obese mice and its possible mechanism. METHODS:   C57 BL/6J mice were fed high‑fat (HF) or low‑fat (LF) diet and then divided into four groups:  obesity with periodontitis (HFP), obesity without periodontitis (HFC), normal mice with periodontitis (LFP) and normal mice without periodontitis (LFC). Serum indicators of renal function, namely serum total protein (TP), albumin (ALB), creatinine (Cr) and blood urea nitrogen (UREA) were measured...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29322805/wound-infections-after-median-sternotomy-treated-by-vac-therapy-summary-of-results-and-risk-factor-analysis
#7
M Hulman, B Bezak, P Artemiou, R Cikrai
INTRODUCTION: The aim of this study is to summarize results and analyze risk factors for the development of wound infection in heart surgery patients after median sternotomy. METHOD: In this retrospective analysis with assessment of multiple risk factors, we examined 143 patients with infection after median sternotomy treated with VAC therapy from total of 4,650 patients operated in our department from 2012 to 2015. RESULTS: Total of 143 patients developed significant SSI treated by VAC therapy following cardiac surgery...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29322804/conservative-management-of-biopsy-confirmed-high-grade-squamous-intraepithelial-lesions
#8
J Hederlingova, Ch W Redman, J Zahumensky
OBJECTIVES: Follow-up of women with biopsy-confirmed CIN2+ who were either treated immediately with LLETZ or managed conservatively to determine the rates of patients back on routine screening programme after a median of three years in two groups. METHODS: In this retrospective study, 310 patients were involved who had undergone biopsy with result of CIN2+ between January 2011 and December 2014. Depending on the management, i.e. based on whether cytology and colposcopy follow-up or immediate treatment were performed, they were divided in two groups...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29322803/is-the-prevalence-of-the-medication-related-osteonecrosis-of-the-jaws-underestimated-evaluation-in-oncological-and-non-oncological-disease
#9
B Galis, J Zajko, D Hirjak, L Vanko, I Kupcova, J Jurkemik, P Gengelova, K Mikuskova, K Halmova, M Riznic, L Czako
OBJECTIVES: The purpose of this study was to evaluate the prevalence of medication-related osteonecrosis of the jaw in Slovak population and compare the literature findings, whether the prevalence of MRONJ is underestimated. BACKGROUND: Antiresorptive drugs significantly increase quality of life, although during therapy, or in post-treatment period, osteonecrosis of the jaws might occur as a severe adverse effect. Medication-related osteonecrosis of the jaws (MRONJ) is a severe problem that has been observed in the past few years...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29322802/cardiac-telocytes-as-principal-interstitial-cells-for-myocardial-reparation-and-regeneration-after-infarction-our-hope
#10
I Varga, J Kyselovic, L Danihel, M Klein, T Barczi, P Galfiova, L Danisovic
According to our knowledge, this is the first research experiment that focuses on the study of the distribution of c-kit positive cells at the sites of myocardial infarction in human hearts (Fig. 3, Ref. 16).
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29322801/paraoxonase-activity-in-sera-of-patients-with-non-alcoholic-fatty-liver-disease
#11
M Fedelesova, V Kupcova, J Luha, L Turecky
The results of our study showed significantly decreased levels of PON1 in patients with chronic liver diseases (controls 185 ± 14 U/l, NAFLD 160 ± 15 U/l, chronic hepatitis 99 ± 18 U/l, cirrhosis 52 ± 11 U/l). There were significant correlations of PON activities with standard liver function tests (Tab. 1, Ref. 5).
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29216730/the-influence-of-cranio-cervical-rehabilitation-in-patients-with-myofascial-temporomandibular-pain-disorders
#12
K Halmova, D Holly, P Stanko
OBJECTIVE: The aim of the study was to prove the causality between the craniocervical dysfunction and myofascial pain in the head and neck and to demonstrate the clinical value and usefulness of physiotherapy as one of the therapeutic options for myofascial pain. METHODS: The group of patients diagnosed with myofascial dysfunctional pain syndrome contained 98 patients out of which 79 patients (81 %) were females and 19 patients (19 %) were males. The majority of the patients were aged between 26 and 35 years; the total age range was 14-77 years with the average of 38 years...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29216729/periostin-normalizes-levels-of-cardiac-markers-in-rats-with-experimental-isoproterenol-cardiotoxicity
#13
A K Devrim, M Sozmen, T Devrim, M Sudagidan, M Cinar, Y B Kabak
BACKGROUND: Although the molecular mechanism of cardiac healing is not fully understood, myocardial infarction is one of the most usual diagnoses in hospitalized patients in industrialized nations while periostin has been recently suggested to have a potential in tissue repairing following myocardial ischemia. OBJECTIVES: To investigate the effects of periostin on the levels of selected cardiac parameters (cardiac troponin I and T, creatine kinase and creatine kinase isoenzyme-MB), antioxidant/lipid peroxidation parameters (superoxide dismutase, catalase, and malondialdehyde), hepatic parameters (alkaline phosphatase, lactate dehydrogenase, aspartate and alanine transaminases) as well as lipids (total cholesterol, triglyceride, high, low and very‑low‑density lipoproteins) in a rat model of isoproterenol---induced myocardial injury...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29216728/optimization-of-eva-green-real-time-mpcr-for-differentiating-c-jejuni-coli-directly-from-feces
#14
M Pavlova, V Velev, E Dobreva, G Asseva, I Ivanov, P Petrov, A Mangarov, I Tomova, T Kantardjiev
OBJECTIVE: To develop and optimize a rapid molecular method for diagnosing campylobacteriosis directly from a clinical fecal sample and at the same time for determining the most common causing agents - C. jejuni/coli. MATERIALS AND METHODS: 38 clinical fecal samples from hospitalized patients with diarrheal syndrome were tested using a rapid immunochromatographic test. All positive samples were tested for confirmation by culturing in a microaerophilic atmosphere...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29216727/effect-of-egf-on-bax-bcl-2-and-fas-expression-in-ulcerous-disease-and-n87-cell-line
#15
F Soylemez, O I Ay, E Ay, E Altintas, C H Turkseven, M E Erdal
BACKGROUND: To evaluate the effect of epithelial growth factor (EGF) in primary culture of ulcer patients and N87 cell line on expressions of apoptotic genes. METHODS: Ulcer patients who attended Gastroenterology Clinic of Mersin University Medical Faculty were included in this study. Three different doses of EGF were applied to the primary culture of biopsy samples from ulcer patients and gastric cancer cell-line (ATCC-NCI-N87) . The expression levels of Bax, Bcl-2 and Fas genes were measured with quantitative real-time PCR (qRT-PCR)...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29216726/high-serum-levels-of-igf-i-and-igfbp3-may-increase-comorbidity-risk-for-asthmatic-patients
#16
M Acat, U Toru Erbay, S Sahin, O Arik, C Ayada
OBJECTIVE: Asthma is known as a chronic inflammatory lung disease which has also systemic features. Insulin-like growth factor I (IGF-I) plays a role for asthma pathogenesis. Controversially, IGF-binding protein 3 (IGFBP3) blocks asthma development. That is why IGF-I and IGFBP3 are targeted for future therapeutic treatments of asthma. We aimed to investigate serum level of IGF-I and IGFBP3 in patients with asthma. This study was performed in 27 asthma and 23 healthy individuals. Serum levels of IGF-I and IGFBP3 were measured by human ELISA assay kits...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29216725/decreased-circulatory-microrna-4478-as-a-specific-biomarker-for-diagnosing-non-st-segment-elevation-myocardial-infarction-nstemi-and-its-association-with-soluble-leptin-receptor
#17
R Gholikhani-Darbroud, F Khaki-Khatibi, F Mansouri, M Hajahmadipoorrafsanjani, M Ghojazadeh
OBJECTIVE: This study aimed to assess the usefulness of circulatory microRNA-4478 (miR-4478) and soluble leptin receptor (sLEPR) in prognosis and diagnosis of non-ST-segment elevation myocardial infarction (NSTEMI) and to introduce miR-4478 as a new biomarker for NSTEMI disease. This study aimed also to examine correlation between miR-4478 and soluble leptin receptor and effects of miR-4478 on leptin receptor concentration. BACKGROUND: MicroRNAs could be used as predictive biomarkers for diseases...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29216724/inhibition-by-egb761-of-the-effect-of-cellphone-radiation-on-the-male-reproductive-system
#18
F Gevrek, D Aydin, S Ozsoy, H Aygun, C Bicer
OBJECTIVES: To determine the effects of Egb761 on testicular tissues and semen parameters in rats exposed to cellphone waves. BACKGROUND: EGb761 has antioxidant properties as a free-radical scavenger. Cellphone electromagnetic radiation (EMR) induces oxidative stress in cells. METHODS: Twenty-one Wistar albino male adult rats were divided into three groups (control, experimental, treatment), including seven rats in each. The experimental and treatment groups were exposed to cellphone EMR (0...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29216723/effect-of-aquaporin-1-and-4-on-masticatory-muscles-degeneration-as-a-result-of-aging
#19
M K Tumer, M Demir, M Cicek
OBJECTIVE: The changes in the mouth structures due to aging cause some structural and functional changes by affecting masticatory muscles over time. The aim of this study was to evaluate the aging-related histopathologic changes and immunohistochemically assessed aquaporin 1 and 4 expressions on masseter and temporal muscles. MATERIAL AND METHODS: 14 Balb/c white mice (50-80 g) were used in this study. Group I consisted of young animals (2-month-individuals) (n = 7) and Group II consisted of older animals (18-month-old) (n = 7)...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29216722/the-influence-of-orthodontic-non-extraction-treatment-on-the-change-in-the-inclination-and-position-of-incisors-in-the-europoid-race
#20
A Koniarova, E Sedlata Juraskova, M Spidlen, D Statelova
AIM: To detect post-treatment change in the inclination and position of incisors in cases treated with orthodontic non- extraction therapy. MATERIALS AND METHODS: The group consisted of 102 patients without extractions in lower and upper dental arch during orthodontic treatment. Cephalogram examination evaluated the position of the lower incisor to point A by Downs-pogonion line (-1 to APo) and inclination of the lower incisor to mandibular line (-1 to ML), position of the upper incisor to nasion-pogonion line (+1 to NPo), inclination of the upper incisor to nasion-sella line (+1 to NS) and the size of the inter-incisival angle between upper and central lower incisor (-1 to +1)...
2017: Bratislavské Lekárske Listy
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