journal
MENU ▼
Read by QxMD icon Read
search

Journal of Cell Science

journal
https://www.readbyqxmd.com/read/27909249/mortalin-mediated-and-erk-controlled-targeting-of-hif-1%C3%AE-to-mitochondria-confers-resistance-to-apoptosis-under-hypoxia
#1
Ilias Mylonis, Maria Kourti, Martina Samiotaki, George Panayotou, George Simos
Hypoxia inducible factor-1 (HIF-1) is the main transcriptional activator of the cellular response to hypoxia and an important target of anticancer therapy. Phosphorylation by ERK stimulates the transcriptional activity of HIF-1α by inhibiting its CRM1-dependent nuclear export. Here, we demonstrate that phosphorylation by ERK also regulates the association of HIF-1α with a novel interaction partner identified as mortalin (GRP75) which mediates non-genomic involvement of HIF-1α in apoptosis. Mortalin binds specifically to HIF-1α lacking modification by ERK and their complex is localized outside the nucleus...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27909248/regulation-of-chemokine-receptor-ccr2-recycling-by-filamin-a-phosphorylation
#2
Mònica Pons, Ismael Izquierdo, Mireia Andreu-Carbó, Georgina Garrido, Jesús Planagumà, Olivia Muriel, M Isabel Geli, Anna M Aragay
Proper endosomal trafficking of ligand-activated G protein-coupled receptors (GPCRs) is essential to spatiotemporally tune their physiological responses. For the monocyte chemoattractant receptor 2 (CCR2B), endocytic recycling is important to sustain monocyte migration; while filamin A (FLNa) is essential for CCL2-induced monocyte migration. Here, we analyze the role of FLNa in the trafficking of CCR2B along the endocytic pathway. In FLNa knockdown cells, activated CCR2B accumulated in enlarged EEA-1-positive endosomes, which exhibited slow movement and fast fluorescence recovery, suggesting an imbalance between receptor entry and exit rates...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27909247/integration-of-transmembrane-domains-is-regulated-by-their-downstream-sequences
#3
Tina Junne, Martin Spiess
The Sec61 translocon catalyzes translocation of proteins into the endoplasmic reticulum and the lateral integration of transmembrane segments into the lipid bilayer. Integration is mediated by the hydrophobicity of a polypeptide segment consistent with thermodynamic equilibration between the translocon and the lipid membrane. Integration efficiency of a generic series of increasingly hydrophobic sequences (H-segments) was found to diverge significantly in different reporter constructs as a function of the ∼100 residues carboxyterminal of the H-segments...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27909246/retromer-wash-dependent-sorting-of-nutrient-transporters-requires-a-multivalent-interaction-network-with-ankrd50
#4
Arunas Kvainickas, Ana Jimenez Orgaz, Heike Nägele, Britta Diedrich, Kate J Heesom, Jörn Dengjel, Peter J Cullen, Florian Steinberg
Retromer and the associated actin polymerizing WASH-complex are essential for the endocytic recycling of a wide range of integral membrane proteins. A hereditary Parkinson's disease causing point mutation (D620N) in the retromer subunit VPS35 perturbs retromer's association with the WASH-complex and also with the uncharacterized protein Ankyrin Repeat Domain Containing Protein 50 (ANKRD50). Here, we firmly establish ANKRD50 as a novel and essential component of the SNX27-retromer-WASH supercomplex. Depletion of ANKRD50 in HeLa or U2OS cells phenocopied the loss of endosome to cell surface recycling of multiple transmembrane proteins seen upon suppression of SNX27, retromer or WASH-complex components...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27909245/heterochromatin-aggregation-during-dna-elimination-in-tetrahymena-is-facilitated-by-a-prion-like-protein
#5
Kensuke Kataoka, Kazufumi Mochizuki
Regulated aggregations of prion and prion-like proteins play physiological roles in various biological processes. However, their structural roles in the nucleus are poorly understood. Here, we show that the prion-like protein Jub6p is involved in the regulation of chromatin structure in the ciliated protozoan Tetrahymena thermophila Jub6p forms SDS-resistant aggregates when it is ectopically expressed in vegetative cells and binds to RNA in vitro. Jub6p is a heterochromatin component and is important for the formation of heterochromatin bodies during the process of programmed DNA elimination...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27909244/cd4-down-regulation-by-hiv-1-nef-reveals-distinct-roles-for-%C3%AE-1-and-%C3%AE-2-subunits-of-ap-1-complex-in-protein-trafficking
#6
Lucas A Tavares, Eulália M L da Silva, Mara E da Silva-Januário, Yunan C Januário, Julianne V de Cavalho, Érika S Czernisz, Gonzalo A Mardones, Luis L P daSilva
The HIV accessory protein Nef is a major determinant of viral pathogenesis that facilitates viral particle release, prevents viral antigen presentation and increases infectivity of new virus particles. These functions of Nef involve its ability to remove specific host proteins from the surface of infected cells, including the CD4 receptor. Nef binds to the adaptor protein 2 (AP-2) and CD4 in clathrin-coated pits, forcing CD4 internalization and subsequent target to lysosomes. Herein, we report that this lysosomal targeting requires a variant of the AP-1 comprising the isoform 2 of γ-adaptin...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27909243/3d-culture-models-of-tissues-under-tension
#7
REVIEW
Jeroen Eyckmans, Christopher S Chen
Cells dynamically assemble and organize into complex tissues during development, and the resulting three-dimensional (3D) arrangement of cells and their surrounding extracellular matrix in turn feeds back to regulate cell and tissue function. Recent advances in engineered cultures of cells to model 3D tissues or organoids have begun to capture this dynamic reciprocity between form and function. Here, we describe the underlying principles that have advanced the field, focusing in particular on recent progress in using mechanical constraints to recapitulate the structure and function of musculoskeletal tissues...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27909242/beneficial-effects-of-rapamycin-in-a-drosophila-model-for-hereditary-spastic-paraplegia
#8
Shiyu Xu, Michael Stern, James A McNew
The locomotor deficits in the hereditary spastic paraplegias (HSPs) reflect degeneration of upper motor neurons, but the mechanisms underlying this neurodegeneration are unknown. We established a Drosophila model for the HSP atlastin (atl), which encodes an ER fusion protein. Here we show that neuronal atl loss causes degeneration of specific thoracic muscles that is preceded by other pathologies including accumulation of aggregates containing poly-ubiquitin (poly-UB), increased generation of reactive oxygen species, and activation of the JNK/Foxo stress response pathway...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27888218/diverting-cert-mediated-ceramide-transport-to-mitochondria-triggers-bax-dependent-apoptosis
#9
Amrita Jain, Oliver Beutel, Katharina Ebell, Sergey Korneev, Joost C M Holthuis
A deregulation of ceramide biosynthesis in the ER is frequently linked to induction of mitochondrial apoptosis. While in vitro studies suggest that ceramides may initiate cell death by acting directly on mitochondria, their actual contribution to the apoptotic response in living cells is unclear. In here, we analyzed the consequences of targeting the biosynthetic flow of ceramides to mitochondria using a ceramide transfer protein equipped with an outer mitochondrial membrane anchor, mitoCERT. Cells expressing mitoCERT import ceramides into mitochondria and undergo Bax-dependent apoptosis...
November 25, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27888217/quantitative-fastfucci-assay-defines-cell-cycle-dynamics-at-single-cell-level
#10
Siang-Boon Koh, Patrice Mascalchi, Esther Rodriguez, Yao Lin, Duncan I Jodrell, Frances M Richards, Scott K Lyons
The fluorescence ubiquitination-based cell cycle indicator (FUCCI) is a powerful tool for use in live cells but current FUCCI-based assays have limited throughput and quantitative robustness. Here, we developed a lentiviral system that rapidly introduced FUCCI transgenes into cells, using an all-in-one expression cassette FastFUCCI. The approach obviated the need for sequential transduction and characterisation, improving labelling efficiency. We coupled the system to an automated imaging workflow capable of handling large datasets...
November 25, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27888216/drosophila-sulf1-is-required-for-the-termination-of-intestinal-stem-cell-division-during-regeneration
#11
Masahiko Takemura, Hiroshi Nakato
Stem cell division is activated to trigger regeneration in response to tissue damage. The molecular mechanisms by which this stem cell mitotic activity is properly repressed at the end of regeneration are poorly understood. Here we show that a specific modification of heparan sulfate (HS) is critical in regulating Drosophila intestinal stem cell (ISC) division during normal midgut homeostasis and regeneration. Loss of the extracellular HS endosulfatase Sulf1 results in increased ISC division during normal homeostasis, which is caused by upregulation of mitogenic signaling including the JAK/STAT, EGFR, and Hedgehog pathways...
November 25, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27888215/super-resolution-microscopy-reveals-the-reorganization-of-glut4-on-plasma-membrane-regulated-by-insulin-resistance
#12
Lan Gao, Junling Chen, Jing Gao, Hongda Wang, Wenyong Xiong
GLUT4 is essential for glucose uptake in skeletal muscles and adipocytes, which play central roles in whole-body glucose metabolism. Here, using direct stochastic optical reconstruction microscopy (dSTORM) to investigate the characteristics of the plasma membrane (PM) fused GLUT4 at the single-molecule level, we demonstrated that insulin and insulin resistance regulated the spatial organization of GLUT4 in adipocytes. Stimulation of insulin shifted the balance of GLUT4 on the PM toward a more dispersed configuration...
November 25, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27884932/drosophila-wash-is-required-for-integrin-mediated-cell-adhesion-cell-motility-and-lysosomal-neutralization
#13
Benedikt M Nagel, Meike Bechtold, Luis Garcia Rodriguez, Sven Bogdan
The Wiskott-Aldrich Syndrome Protein and SCAR Homologue (WASH) is a conserved actin nucleation promoting factor controlling Arp2/3 complex activity in endosomal sorting and recycling. Previous studies have identified WASH as an essential regulator in Drosophila development. Here, we show that homozygous wash mutant flies are viable and fertile. We demonstrate that Drosophila WASH has conserved functions in integrin receptor recycling and lysosome neutralization. WASH generates actin patches on endosomes and lysosomes mediating both functions...
November 24, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27872153/xpf-suppresses-mutagenic-consequences-of-bacterial-phagocytosis-in-dictyostelium
#14
Lucas B Pontel, Judith Langenick, Ivan V Rosado, Xiao-Yin Zhang, David Traynor, Robert R Kay, Ketan J Patel
As time passes, mutations accumulate in the genomes of all living organisms. These changes promote genetic diversity, but also precipitate ageing and the initiation of cancer. Food is a common source of mutagens, but little is known about how nutritional factors cause lasting genetic changes in the consuming organism. Here, we describe an unusual genetic interaction between DNA repair in the unicellular amoeba - Dictyostelium discoideum - and its natural bacterial food source. Dictyostelium deficient in the DNA repair nuclease Xpf displays a severe and specific growth defect when feeding on bacteria...
November 21, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27872152/an-unconventional-interaction-between-dis1-tog-and-mal3-eb1-promotes-the-fidelity-of-chromosome-segregation
#15
Yuzy Matsuo, Sebastian P Maurer, Masashi Yukawa, Silva Zakian, Martin R Singleton, Thomas Surrey, Takashi Toda
Dynamic microtubule plus ends interact with various intracellular target regions such as the cell cortex and the kinetochore. Two conserved families of microtubule plus-end tracking proteins, XMAP215/TOG and EB1, play pivotal roles in regulating microtubule dynamics. Here we study the functional interplay between fission yeast Dis1/XMAP215 and Mal3/EB1. Using an in vitro microscopy assay, we find that purified Dis1 autonomously tracks growing microtubule ends and is a bona fide microtubule polymerase. Mal3 recruits additional Dis1 to microtubule ends, explaining the synergistic enhancement of microtubule dynamicity by these proteins...
November 21, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27856508/how-cells-respond-to-environmental-cues-insights-from-bio-functionalized-substrates
#16
REVIEW
Verena Ruprecht, Pascale Monzo, Andrea Ravasio, Zhang Yue, Ekta Makhija, Pierre Olivier Strale, Nils Gauthier, G V Shivashankar, Vincent Studer, Corinne Albiges-Rizo, Virgile Viasnoff
Biomimetic materials have long been the (he)art of bioengineering. They usually aim at mimicking in vivo conditions to allow in vitro culture, differentiation and expansion of cells. The past decade has witnessed a considerable amount of progress in soft lithography, bio-inspired micro-fabrication and biochemistry, allowing the design of sophisticated and physiologically relevant micro- and nano-environments. These systems now provide an exquisite toolbox with which we can control a large set of physicochemical environmental parameters that determine cell behavior...
November 17, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27856507/perforating-the-nuclear-boundary-how-nuclear-pore-complexes-assemble
#17
REVIEW
Marion Weberruss, Wolfram Antonin
The nucleus is enclosed by the nuclear envelope, a double membrane which creates a selective barrier between the cytoplasm and the nuclear interior. Its barrier and transport characteristics are determined by nuclear pore complexes (NPCs) that are embedded within the nuclear envelope, and control molecular exchange between the cytoplasm and nucleoplasm. In this Commentary, we discuss the biogenesis of these huge protein assemblies from approximately one thousand individual proteins. We will summarize current knowledge about distinct assembly modes in animal cells that are characteristic for different cell cycle phases and their regulation...
November 17, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27852836/perturbations-in-actin-dynamics-reconfigure-protein-complexes-that-modulate-gcn2-activity-and-promote-an-eif2-response
#18
Richard C Silva, Evelyn Sattlegger, Beatriz A Castilho
Genetic and pharmacological interventions in yeast and mammalian cells have suggested a cross-talk between the actin cytoskeleton and protein synthesis. Regulation of the activity of the translation initiation factor 2 (eIF2) is a paramount mechanism for cells to rapidly adjust the rate of protein synthesis and to trigger reprogramming of gene expression to adapt in response to internal and external cues. Here we show that disruption of F-actin in mammalian cells inhibits translation in a GCN2-dependent manner, correlating with increased levels of uncharged tRNA...
November 16, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27852835/mzt1-regulates-microtubule-nucleation-by-linking-%C3%AE-turc-assembly-to-adapter-mediated-targeting-and-activation
#19
Rosa Ramírez Cota, Neus Teixidó-Travesa, Artur Ezquerra, Susana Eibes, Cristina Lacasa, Joan Roig, Jens Lüders
Regulation of the γ-tubulin ring complex (γTuRC) through targeting and activation restricts nucleation of microtubules to microtubule organizing centers (MTOCs), aiding in the assembly of ordered microtubule arrays. However, the mechanistic basis of this important regulation remains poorly understood. Here we show that in human cells γTuRC integrity, determined by the presence of γ-tubulin complex proteins (GCPs) 2-6, is a prerequisite for interaction with the targeting factor NEDD1, impacting on essentially all γ-tubulin dependent functions...
November 16, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27875278/ratiometric-analysis-of-acridine-orange-staining-in-the-study-of-acidic-organelles-and-autophagy
#20
Marcos Paulo Thomé, Eduardo Cremonese Filippi-Chiela, Emilly Schlee Villodre, Celina Borges Migliavaca, Giovana Ravizzoni Onzi, Karina Bettega Felipe, Guido Lenz
Acridine orange (AO) is a cell permeable green fluorophore that can be protonated and trapped in Acidic Vesicular Organelles (AVOs). Its metachromatic shift to red fluorescence is concentration-dependent and, therefore, AO fluoresces red in AVOs, such as autolysosomes. This makes AO staining a quick, accessible and reliable method to assess the volume of AVOs, which increases upon autophagy induction. Here we describe a ratiometric analysis of autophagy using AO, considering the red-to-green fluorescence intensity ratio (R/GFIR) to quantify flow cytometry and fluorescence microscopy data of AO-stained cells...
November 14, 2016: Journal of Cell Science
journal
journal
21391
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"