journal
MENU ▼
Read by QxMD icon Read
search

Journal of Cell Science

journal
https://www.readbyqxmd.com/read/28646093/emerging-roles-of-mechanical-forces-in-chromatin-regulation
#1
REVIEW
Yekaterina A Miroshnikova, Michele M Nava, Sara A Wickström
Cells are constantly subjected to a spectrum of mechanical cues, such as shear stress, compression, differential tissue rigidity and strain, to which they adapt by engaging mechanisms of mechanotransduction. While the central role of cell adhesion receptors in this process is established, it has only recently been appreciated that mechanical cues reach far beyond the plasma membrane and the cytoskeleton, and are directly transmitted to the nucleus. Furthermore, changes in the mechanical properties of the perinuclear cytoskeleton, nuclear lamina and chromatin are critical for cellular responses and adaptation to external mechanical cues...
June 23, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28646092/deletion-analysis-of-agd1-reveals-domains-crucial-for-its-plasma-membrane-recruitment-and-function-in-root-hair-polarity
#2
Cheol-Min Yoo, Satoshi Naramoto, J Alan Sparks, Bibi Rafeiza Khan, Jin Nakashima, Hiroo Fukuda, Elison B Blancaflor
AGD1, a plant ACAP-type ADP-ribosylation factor-GTPase activating protein (ARF-GAP), functions in specifying root hair polarity in Arabidopsis thaliana To better understand how AGD1 modulates root hair growth, we generated full length and domain-deleted AGD1-green fluorescent protein (GFP) constructs, and followed their localization during root hair development. AGD1-GFP localized to the cytoplasm and was recruited to specific regions of the root hair plasma membrane (PM). Distinct PM AGD1-GFP signal was first detected along the site of root hair bulge formation...
June 23, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28646091/stress-specific-p38-map-kinase-activation-is-sufficient-to-drive-egf-receptor-endocytosis-but-not-nuclear-translocation
#3
Alejandra Tomas, Sylwia Jones, Simon O Vaughan, Daniel Hochhauser, Clare E Futter
P38 MAP kinase-dependent EGF receptor (EGFR) endocytosis is induced by stress. Ligand and stresses like X-rays, reportedly promote nuclear traffic of endocytosed EGFR for regulation of gene transcription and DNA repair. We fail to detect EGFR endocytosis or nuclear transport following X-ray treatment of HeLa or head and neck cancer cells, despite extensive DNA damage induction. Apparent nuclear staining with EGFR extracellular domain antibody remained present despite reduced/absent EGFR expression and so did not represent nuclear EGFR...
June 23, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28646090/cellular-functions-of-wasp-family-proteins-at-a-glance
#4
REVIEW
Olga Alekhina, Ezra Burstein, Daniel D Billadeau
Proteins of the Wiskott-Aldrich syndrome protein (WASP) family function as nucleation-promoting factors for the ubiquitously expressed Arp2/3 complex, which drives the generation of branched actin filaments. Arp2/3-generated actin regulates diverse cellular processes, including the formation of lamellipodia and filopodia, endocytosis and/or phagocytosis at the plasma membrane, and the generation of cargo-laden vesicles from organelles including the Golgi, endoplasmic reticulum (ER) and the endo-lysosomal network...
June 23, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28630166/deficiency-of-phb-complex-impairs-respiratory-supercomplex-formation-and-activates-mitochondrial-flashes
#5
Chongshu Jian, Fengli Xu, Tingting Hou, Tao Sun, Li Jinghang, Heping Cheng, Xianhua Wang
Prohibitins (prohibitin 1, PHB1, and prohibitin 2, PHB2) are evolutionally conserved and ubiquitously expressed mitochondrial protein. PHBs form multimeric ring complexes acting as scaffolds in the inner mitochondrial membrane. Mitochondrial flashes (mitoflashes) are newly discovered mitochondrial signaling events which reflect electrical and chemical excitations of the organelle. Here we investigate possible roles of PHBs in the regulation of mitoflash signaling. Down-regulation of PHBs increases mitoflash frequency by up to 5...
June 19, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28630165/the-cell-adhesion-molecule-chl1-interacts-with-patched-1-to-regulate-apoptosis-during-postnatal-cerebellar-development
#6
Jelena Katic, Gabriele Loers, Jelena Tosic, Melitta Schachner, Ralf Kleene
The immunoglobulin superfamily adhesion molecule close homolog of L1 (CHL1) plays important roles during nervous system development. Here, we identified the hedgehog receptor patched-1 (PTCH1) as novel CHL1 binding partner and showed that CHL1 interacts with the first extracellular loop of PTCH1 via its extracellular domain. Co-localization and -immunoprecipitation of CHL1 with PTCH1 suggest an association of CHL1 with this major component of the hedgehog signaling pathway. The trans-interaction of CHL1 with PTCH1 promotes neuronal survival in cultures of dissociated cerebellar granule cells and of organotypic cerebellar slices...
June 19, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28615413/breaking-the-color-barrier-a-multi-selective-antibody-reporter-offers-innovative-strategies-of-fluorescence-detection
#7
Eugenio Gallo, Jonathan W Jarvik
A novel bi-partite fluorescence platform exploits the high affinity and selectivity of antibody scaffolds to capture and activate small molecule fluorogens. In this report we investigated the property of multi-selectivity activation from a single antibody against diverse cyanine-family fluorogens. Our fluorescence screen identified three cell impermeant fluorogens; each with unique emission spectra (blue, green, and red) and nanomolar affinities. Most importantly, as a protein fusion-tag to G-protein-coupled receptors the antibody biosensor retained full activity - displaying bright fluorogen signals with minimal background on live cells...
June 14, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28615412/insights-into-cortical-microtubule-nucleation-and-dynamics-in-arabidopsis-leaf-cells
#8
Noriyoshi Yagi, Sachihiro Matsunaga, Takashi Hashimoto
Plant microtubules (MTs) are nucleated from the γ-tubulin-containing ring complex (γTuRC). In cortical MT arrays of interphase plant cells, γTuRC is preferentially recruited to the lattice of preexisting MTs, where it initiates MT nucleation in either a branch- or bundle-forming manner, or dissociates without nucleation. In this study, we analyzed how γTuRCs influence MT nucleation and dynamics in cotyledon pavement cells of Arabidopsis thaliana We found that γTuRC nucleated MTs at angles of approximately 40 degrees toward the plus-ends of existing MTs, or in predominantly antiparallel bundles...
June 14, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28606991/caspase-digestion-resistant-homozygous-keratin-18-mutation-protects-mouse-liver-from-apoptosis-and-injury
#9
Han-Na Yoon, Sung Yeon Yoon, Jin Hyuck Hong, Nam-On Ku
Cytoskeletal keratin 18 (K18) undergoes caspase-mediated digestion during apoptosis, which leads to dramatic keratin filament disassembly. We studied the significance of K18 caspase digestion in a mouse model. We generated transgenic mice expressing human K18 caspase digestion-resistant double-mutant, K18-D238/397E, in mouse (m) K18-null background, and compared their response to Fas-mediated injury. Notably, K18-D238/397E;mK18-null mice were significantly more resistant to Fas-induced injury as compared with K18-WT;mK18-null mice (23% vs 57% lethality, respectively; p<0...
June 12, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28606990/the-formin-daam-is-required-for-coordination-of-the-actin-and-microtubule-cytoskeleton-in-axonal-growth-cones
#10
Szilárd Szikora, István Földi, Krisztina Tóth, Ede Migh, Andrea Vig, Beáta Bugyi, József Maléth, Péter Hegyi, Péter Kaltenecker, Natalia Sanchez-Soriano, József Mihály
Directed axonal growth depends on proper coordination of the actin and microtubule cytoskeleton in the growth cone. However, despite the relatively large number of proteins implicated in actin-microtubule cross-talk, the mechanisms whereby actin polymerization is coupled to microtubule stabilization and advancement in the peripheral growth cone remained largely unclear. Here we identified the formin DAAM as a novel factor playing a role in concerted regulation of actin and microtubule remodeling in Drosophila primary neurons...
June 12, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28600325/hira-deficiency-in-muscle-fibers-causes-hypertrophy-and-susceptibility-to-oxidative-stress
#11
Nicolas Valenzuela, Benjamin Soibam, Lerong Li, Jing Wang, Lauren A Byers, Yu Liu, Robert J Schwartz, M David Stewart
Nucleosome assembly proceeds through DNA replication-coupled or replication-independent mechanisms. For skeletal myocytes, whose nuclei have permanently exited the cell cycle, replication-independent assembly is the only mode available for chromatin remodeling. For this reason, any nucleosome composition alterations accompanying transcriptional responses to physiological signals must occur through a DNA replication-independent pathway. HIRA is the histone chaperone primarily responsible for replication-independent incorporation of histone variant H3...
June 9, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28600324/reconstitution-of-the-oocyte-nucleolus-in-mice-by-a-single-nucleolar-protein-npm2
#12
Sugako Ogushi, Kazuo Yamagata, Chikashi Obuse, Keiko Furuta, Teruhiko Wakayama, Martin M Matzuk, Mitinori Saitou
The mammalian oocyte nucleolus, the most prominent subcellular organelle in the oocyte, is vital in early development, yet its key functions and constituents remain unclear. We show here that the parthenotes/zygotes derived from enucleolated oocytes exhibited abnormal heterochromatin formation around parental peri-centromeric DNAs, which led to a significant mitotic delay and frequent chromosome mis-segregation upon the first mitotic division. A proteomic analysis identified NPM2 as a dominant component of the oocyte nucleolus...
June 9, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28600323/rab7-palmitoylation-is-required-for-efficient-endosome-to-tgn-trafficking
#13
Graziana Modica, Olga Skorobogata, Etienne Sauvageau, Adriano Vissa, Christopher M Yip, Peter K Kim, Hugo Wurtele, Stephane Lefrancois
Retromer is a multimeric protein complex that mediates endosome-to-TGN and endosome-to-plasma membrane trafficking of integral membrane proteins. Dysfunction of this complex has been linked to Alzheimer's and Parkinson's disease. The recruitment of retromer to endosomes is regulated by Rab7 to coordinate endosome-to- TGN trafficking of cargo-receptor complexes. Rab7 is also required for the degradation of internalized integral membrane proteins such as the epidermal growth factor receptor. We found that Rab7 is palmitoylated and that this modification is not required for membrane anchoring...
June 9, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28600322/rac1-switching-at-the-right-time-and-location-is-essential-for-fc%C3%AE-receptor-mediated-phagosome-formation
#14
Yuka Ikeda, Katsuhisa Kawai, Akira Ikawa, Kyoko Kawamoto, Youhei Egami, Nobukazu Araki
Lamellipodia are sheet-like cell protrusions driven by actin polymerization mainly through Rac1, a GTPase molecular switch. In Fcγ receptor-mediated phagocytosis of IgG-opsonized erythrocytes (IgG-Es), Rac1 activation is required for lamellipodial extension along the surface of IgG-Es. However, the significance of Rac1 deactivation in phagosome formation is poorly understood. Our live-cell imaging and electron microscopy revealed that RAW264 macrophages expressing a constitutively active Rac1 mutant showed defects in phagocytic cup formation, while lamellipodia were formed around IgG-Es...
June 9, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28600321/importin-beta-and-crm1-control-a-ranbp2-spatiotemporal-switch-essential-for-mitotic-kinetochore-function
#15
Eugenia Gilistro, Valeria de Turris, Michela Damizia, Annalisa Verrico, Sara Moroni, Riccardo De Santis, Alessandro Rosa, Patrizia Lavia
Protein conjugation with SUMO is a post-translational modification that modulates protein interactions and localisation. RANBP2 is a large nucleoporin endowed with SUMO E3 ligase and SUMO-stabilising activity and is implicated in some cancer types. RANBP2 is part of a larger complex, comprising SUMO-modified RANGAP1, the GTP-hydrolysis activating factor for the GTPase RAN. During mitosis, the RANBP2/SUMO-RANGAP1 complex localises to the mitotic spindle and to kinetochores after microtubule attachment. Here we have addressed the mechanisms that regulate this localisation and how they affect kinetochore functions...
June 9, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28600320/limited-distal-organelles-and-synaptic-function-in-extensive-monoaminergic-innervation
#16
Juan Tao, Dinara Bulgari, David L Deitcher, Edwin S Levitan
Organelles such as neuropeptide-containing dense-core vesicles (DCVs) and mitochondria travel down axons to supply synaptic boutons. DCV distribution among en passant boutons in small axonal arbors is mediated by circulation with bidirectional capture. However, it is not known how organelles are distributed in extensive arbors associated with volume transmission and neuromodulation by monoamines and neuropeptides and mammalian dopamine neuron vulnerability. Therefore, we studied presynaptic organelle distribution in Drosophila octopamine neurons that innervate ∼20 muscles with ∼1500 boutons...
June 9, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28596242/smooth-muscle-function-and-myosin-polymerization
#17
Pasquale Chitano, Lu Wang, Gabrielle Y Y Tin, Mitsuo Ikebe, Peter D Paré, Chun Y Seow
Smooth muscle is able to function over a much broader length range than striated muscle. The ability to maintain contractility after a large length change is thought to be due to an adaptive process involving restructuring of the contractile apparatus to maximize overlap between the contractile filaments. The molecular mechanism for the length-adaptive behavior is largely unknown. In smooth muscle adapted to different lengths we quantified myosin monomers, basal and activation-induced myosin light chain (MLC) phosphorylation, shortening-velocity, power-output and active force...
June 8, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28596241/disruption-of-rab8a-and-rab11a-causes-formation-of-basolateral-microvilli-in-neonatal-enteropathy
#18
Qiang Feng, Edward M Bonder, Amy C Engevik, Lanjing Zhang, Matthew J Tyska, James R Goldenring, Nan Gao
Misplaced formation of microvilli to basolateral domains and intracellular inclusions in enterocytes are pathognomonic features in congenital enteropathy associated with STX3 mutation. While the demonstrated binding of Myo5b to the Rab8a and Rab11a small GTPases in vitro implicates cytoskeleton-dependent membrane sorting, the mechanisms underlying the microvillar location defect remain unclear. By selective or combinatory disruption of Rab8a and Rab11a membrane traffic in vivo, we demonstrate that transport of distinct cargo to the apical brush-border rely on either individual or both Rab regulators, whereas certain basolateral cargos are redundantly transported by both factors...
June 8, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28596240/control-of-mitochondrial-homeostasis-by-endocytic-regulatory-proteins
#19
Trey Farmer, James B Reinecke, Shuwei Xie, Kriti Bahl, Naava Naslavsky, Steve Caplan
Mitochondria play essential roles in cellular energy processes, including ATP production, control of ROS, and apoptosis. While mitochondrial function is regulated by the dynamics of fusion and fission, mitochondrial homeostasis remains incompletely understood. Recent studies implicate Dynamin-2 and dynamin-related protein-1 (Drp1), as GTPases involved in mitochondrial fission. We identify the ATPase and endocytic protein, EHD1, as a novel regulator of mitochondrial fission. EHD1-depletion induces a static and elongated network of mitochondria in the cell...
June 8, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28596239/electrophoresis-of-cell-membrane-heparan-sulfate-regulates-galvanotaxis-in-glial-cells
#20
Yu-Ja Huang, Paula Schiapparelli, Kristen Kozielski, Jordan Green, Emily Lavell, Hugo Guerrero-Cazares, Alfredo Quinones-Hinojosa, Peter Searson
Endogenous electric fields modulate many physiological processes by promoting directional migration, a process known as galvanotaxis. Despite the importance of galvanotaxis in development and disease, the mechanism by which cells sense and migrate directionally in an electric field remains unknown. Here we show that electrophoresis of cell surface heparan sulfate (HS) critically regulates this process. HS was found to be localized at the anode-facing side in fetal neural progenitor cells (fNPC), fNPC-derived astrocytes, and brain tumor initiating cells (BTICs), regardless of their direction of galvanotaxis...
June 8, 2017: Journal of Cell Science
journal
journal
21391
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"