journal
MENU ▼
Read by QxMD icon Read
search

Journal of Cell Science

journal
https://www.readbyqxmd.com/read/28720596/elongator-regulates-hypocotyl-growth-in-darkness-and-during-photomorphogenesis
#1
Magdalena Woloszynska, Olimpia Gagliardi, Filip Vandenbussche, Steven De Groeve, Luis Alonso Baez, Pia Neyt, Sabine Le Gall, Jorge Fung, Paloma Mas, Dominique Van Der Straeten, Mieke Van Lijsebettens
Elongator promotes RNA polymerase II-mediated transcript elongation through epigenetic activities such as histone acetylation. Elongator regulates growth, development, immune response, sensitivity to drought and abscisic acid. We demonstrate that elo mutants exhibit defective hypocotyl elongation but have a normal apical hook in darkness and are hyposensitive to light during photomorphogenesis. These elo phenotypes are supported by transcriptome changes, i.e. downregulation of circadian clock components, positive regulators of skoto- or photomorphogenesis, hormonal pathways and cell wall biogenesis-related factors...
July 18, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28705837/cyb-1-a-c-elegans-b-type-cyclin-maintains-proper-position-and-number-of-centrosomes-during-spermatogenesis
#2
Sunghee Yoon, Ichiro Kawasaki, Yhong-Hee Shim
Depletion of cyb-1, a major B-type cyclin expressed during C. elegans spermatogenesis, caused a meiotic division arrest in diakinesis-stage spermatocytes with multiple and mispositioned centrosomes. Association of SUN-1 and ZYG-12, two nuclear membrane proteins, is critical for centrosome-nuclear envelope attachment. We found that sun-1 depletion caused centrosome defects similar to those caused by cyb-1 depletion in diakinesis-stage spermatocytes. In addition, Ser8 and Ser43 residues in SUN-1 were dephosphorylated in cyb-1-depleted diakinesis-stage spermatocytes...
July 13, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28705836/essential-and-selective-role-of-snx12-in-transport-of-endocytic-and-retrograde-cargo
#3
Amulya Priya, Jini Sugatha, Sameena Parveen, Sandra Lacas-Gervais, Prateek Raj, Jérôme Gilleron, Sunando Datta
The endosomal protein sorting machineries play vital roles in diverse physiologically important cellular processes. Much of the core membrane sorting apparatus are conserved in evolution, such as retromer, involved in the recycling of a diverse set of cargoes via retrograde trafficking route. Here, using a RNAi based loss of function study, we identified that SNX12 when suppressed, leads to severe blockage in CIM6PR transport and alters the morphology of the endocytic compartments. We demonstrate that SNX12 is involved in the early phase of CIM6PR transport and mediates receptor recycling upstream of the other well established SNX components of retromer...
July 13, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28701514/talin-the-master-of-integrin-adhesions
#4
REVIEW
Benjamin Klapholz, Nicholas H Brown
Talin has emerged as the key cytoplasmic protein that mediates integrin adhesion to the extracellular matrix. In this Review, we draw on experiments performed in mammalian cells in culture and Drosophila to present evidence that talin is the most important component of integrin adhesion complexes. We describe how the properties of this adaptor protein enable it to orchestrate integrin adhesions. Talin forms the core of integrin adhesion complexes by linking integrins directly to actin, increasing the affinity of integrin for ligands (integrin activation) and recruiting numerous proteins...
July 12, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28701513/the-prion-protein-regulates-glutamate-mediated-ca-2-entry-and-mitochondrial-ca-2-accumulation-in-neurons
#5
Agnese De Mario, Caterina Peggion, Maria Lina Massimino, Francesca Viviani, Angela Castellani, Marta Giacomello, Dmitry Lim, Alessandro Bertoli, Maria Catia Sorgato
The prion protein, PrP(C), whose conformational misfolding originates deadly prions, has a still unclarified cellular function despite decades of intensive research. Following our recent finding that PrP(C) limits Ca(2+) entry via store-operated Ca(2+) channels in neurons, we investigated whether the protein could also control the activity of ionotropic glutamate receptors (iGluRs). To this end, we compared local Ca(2+) movements in primary cerebellar granule neurons and cortical neurons, expressing, or not, PrP(C), transduced with genetically encoded Ca(2+) probes...
July 12, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28687624/patellins-are-regulators-of-auxin-mediated-pin1-relocation-and-plant-development-in-arabidopsis-thaliana
#6
Ricardo Tejos, Cecilia Rodriguez-Furlan, Maciej Adamowski, Michael Sauer, Lorena Norambuena, Jiří Friml
Coordinated cell polarization in developing tissues is a recurrent theme in multicellular organisms. In plants, a directional distribution of the plant hormone auxin is at the core of many developmental programs. A feedback regulation of auxin on the polarized localization of PIN auxin transporters in individual cells has been proposed as a self-organizing mechanism for coordinated tissue polarization, but the molecular mechanisms linking auxin signalling to PIN-dependent auxin transport remain unknown. We performed a microarray-based approach to find regulators of the auxin-induced PIN relocation in the Arabidopsis thaliana root...
July 7, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28687623/nonmuscle-myosin-ii-b-regulates-epicardial-integrity-and-epicardial-derived-mesenchymal-cell-maturation
#7
Xuefei Ma, Derek C Sung, Yanqin Yang, Yoshi Wakabayashi, Robert S Adelstein
Nonmuscle myosin II-B (NMII-B) is essential for cardiac myocyte cytokinesis. The role of NMII-B in other cardiac cells is not known. Here we show that NMII-B is required in epicardial formation and function to support myocardial proliferation and coronary vessel development. Ablation of NMII-B in epicardial cells results in disruption of epicardial integrity with a loss of E-cadherin at cell-cell junctions and a focal detachment of epicardial cells from the myocardium. NMII-B ablated or blebbistatin inhibited epicardial explants demonstrate impaired mesenchymal cell maturation during epicardial epithelial-mesenchymal transition...
July 7, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28687622/polyphosphate-as-donor-of-high-energy-phosphate-for-the-synthesis-of-adp-and-atp
#8
Werner E G Müller, Shunfeng Wang, Meik Neufurth, Maria Kokkinopoulou, Qingling Feng, Heinz C Schröder, Xiaohong Wang
We studied the potential role of inorganic polyphosphate (polyP) as an energy source for ADP and ATP formation in the extracellular space. Using SaOS-2 we show that matrix vesicles are released into the extracellular space after incubation with polyP. These vesicles contain both the alkaline phosphatase (ALP) and the adenylate kinase (AK). Both enzymes translocate to the cell membrane in response to polyP. To distinguish the process(es) (i) AMP and ADP formation during ALP hydrolysis, from the (ii) ATP generation via the AK reaction, inhibition studies with the AK inhibitor A(5')P5(5')A were performed...
July 7, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28679697/spectraplakin-family-proteins-cytoskeletal-crosslinkers-with-versatile-roles
#9
REVIEW
Jamie Zhang, Jiping Yue, Xiaoyang Wu
The different cytoskeletal networks in a cell are responsible for many fundamental cellular processes. Current studies have shown that spectraplakins, cytoskeletal crosslinkers that combine features of both the spectrin and plakin families of crosslinkers, have a critical role in integrating these different cytoskeletal networks. Spectraplakin genes give rise to a variety of isoforms that have distinct functions. Importantly, all spectraplakin isoforms are uniquely able to associate with all three elements of the cytoskeleton, namely, F-actin, microtubules and intermediate filaments...
July 5, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28676501/lifespan-extension-by-peroxidase-dual-oxidase-mediated-ros-signaling-through-pyrroloquinoline-quinone-in-c-elegans
#10
Hiroyuki Sasakura, Hiroki Moribe, Masahiko Nakano, Kazuto Ikemoto, Kosei Takeuchi, Ikue Mori
Reactive oxygen species (ROS), originally characterized based on their harmful effects on cells or organisms, are now recognized as important signal molecules regulating various biological processes. In particular, low levels of ROS released from mitochondria extend lifespan. Here, we identified a novel mechanism of generating appropriate levels of ROS at the plasma membrane through a peroxidase/dual oxidase system, which could extend lifespan in Caenorhabditis elegans (C. elegans). A redox co-factor, pyrroloquinoline quinone (PQQ), activates dual oxidase (DUOX)/BLI-3 to produce the ROS H2O2 at the plasma membrane, which is subsequently degraded by peroxidase/MLT-7, eventually ensuring the adequate levels of ROS...
July 4, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28674075/clathrin-mediated-post-fusion-membrane-retrieval-influences-the-exocytic-mode-of-endothelial-weibel-palade-bodies
#11
Nicola L Stevenson, Ian J White, Jessica J McCormack, Christopher Robinson, Daniel F Cutler, Thomas D Nightingale
Weibel Palade bodies (WPB), the storage organelles of endothelial cells, are essential to normal haemostatic and inflammatory responses. Their major content protein is Von Willebrands factor (VWF) that, following secretagogue-stimulation, is released into the blood vessel lumen as large platelet-catching strings. This exocytosis changes the protein composition of the cell surface and also results in a net increase in the amount of plasma membrane. Compensatory endocytosis is thought to limit changes in cell size and retrieve fusion machinery and other misplaced integral membrane proteins following exocytosis, however little is known about the extent, timing, mechanism and precise function of compensatory endocytosis in endothelial cells...
July 3, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28663386/improved-akt-reporter-reveals-intra-and-inter-cellular-heterogeneity-and-oscillations-in-signal-transduction
#12
Dougall M Norris, Pengyi Yang, James R Krycer, Daniel J Fazakerley, David E James, James G Burchfield
Akt is a key node in a range of signal transduction cascades and plays a critical role in diseases such as cancer and diabetes. Fluorescently-tagged Akt reporters have been used to discern Akt localisation, yet it has not been clear how well these tools recapitulate the behaviour of endogenous Akt. Here we observed that fusion of eGFP to Akt impaired both insulin-stimulated plasma membrane recruitment and its phosphorylation. Endogenous-like responses were restored by replacing eGFP with TagRFP-T. The improved response magnitude and sensitivity afforded by TagRFP-T-Akt over eGFP-Akt enabled monitoring of signalling outcomes in single cells at physiological doses of insulin with subcellular resolution and revealed two previously unreported features of Akt biology...
June 29, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28663385/re-examining-the-role-of-cdc14-phosphatase-in-reversal-of-cdk-phosphorylation-during-mitotic-exit
#13
Brendan L Powers, Mark C Hall
Inactivation of cyclin-dependent kinase (Cdk) and reversal of Cdk phosphorylation are universally required for mitotic exit. In budding yeast, Cdc14 is essential for both and thought to be the major Cdk-counteracting phosphatase. However, Cdc14 is not required for mitotic exit in many eukaryotes, despite highly conserved biochemical properties. The question of how similar enzymes could have such disparate influences on mitotic exit prompted us to re-examine the contribution of budding yeast Cdc14. Using an auxin-inducible degron, severe Cdc14 depletion had no effect on the kinetics of mitotic exit and bulk Cdk substrate dephosphorylation, but caused a cell separation defect and was ultimately lethal...
June 29, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28655854/the-effect-of-sterol-structure-upon-clathrin-mediated-and-clathrin-independent-endocytosis
#14
JiHyun Kim, Ashutosh Singh, Maurizio DelPoeta, Deborah A Brown, Erwin London
Ordered lipid domains (rafts) in plasma membranes have been hypothesized to participate in endocytosis based on inhibition of endocytosis by removal or sequestration of cholesterol. To more carefully investigate the role of the sterol in endocytosis, we used a substitution strategy to replace cholesterol with sterols having various raft-forming abilities and chemical structures. Both clathrin-mediated endocytosis of transferrin and clathrin-independent endocytosis of clustered placental alkaline phosphatase were measured...
June 27, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28646092/deletion-analysis-of-agd1-reveals-domains-crucial-for-its-plasma-membrane-recruitment-and-function-in-root-hair-polarity
#15
Cheol-Min Yoo, Satoshi Naramoto, J Alan Sparks, Bibi Rafeiza Khan, Jin Nakashima, Hiroo Fukuda, Elison B Blancaflor
AGD1, a plant ACAP-type ADP-ribosylation factor-GTPase activating protein (ARF-GAP), functions in specifying root hair polarity in Arabidopsis thaliana To better understand how AGD1 modulates root hair growth, we generated full length and domain-deleted AGD1-green fluorescent protein (GFP) constructs, and followed their localization during root hair development. AGD1-GFP localized to the cytoplasm and was recruited to specific regions of the root hair plasma membrane (PM). Distinct PM AGD1-GFP signal was first detected along the site of root hair bulge formation...
June 23, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28646091/stress-specific-p38-map-kinase-activation-is-sufficient-to-drive-egf-receptor-endocytosis-but-not-nuclear-translocation
#16
Alejandra Tomas, Sylwia Jones, Simon O Vaughan, Daniel Hochhauser, Clare E Futter
P38 MAP kinase-dependent EGF receptor (EGFR) endocytosis is induced by stress. Ligand and stresses like X-rays, reportedly promote nuclear traffic of endocytosed EGFR for regulation of gene transcription and DNA repair. We fail to detect EGFR endocytosis or nuclear transport following X-ray treatment of HeLa or head and neck cancer cells, despite extensive DNA damage induction. Apparent nuclear staining with EGFR extracellular domain antibody remained present despite reduced/absent EGFR expression and so did not represent nuclear EGFR...
June 23, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28630166/deficiency-of-phb-complex-impairs-respiratory-supercomplex-formation-and-activates-mitochondrial-flashes
#17
Chongshu Jian, Fengli Xu, Tingting Hou, Tao Sun, Li Jinghang, Heping Cheng, Xianhua Wang
Prohibitins (prohibitin 1, PHB1, and prohibitin 2, PHB2) are evolutionally conserved and ubiquitously expressed mitochondrial protein. PHBs form multimeric ring complexes acting as scaffolds in the inner mitochondrial membrane. Mitochondrial flashes (mitoflashes) are newly discovered mitochondrial signaling events which reflect electrical and chemical excitations of the organelle. Here we investigate possible roles of PHBs in the regulation of mitoflash signaling. Down-regulation of PHBs increases mitoflash frequency by up to 5...
June 19, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28630165/the-cell-adhesion-molecule-chl1-interacts-with-patched-1-to-regulate-apoptosis-during-postnatal-cerebellar-development
#18
Jelena Katic, Gabriele Loers, Jelena Tosic, Melitta Schachner, Ralf Kleene
The immunoglobulin superfamily adhesion molecule close homolog of L1 (CHL1) plays important roles during nervous system development. Here, we identified the hedgehog receptor patched-1 (PTCH1) as novel CHL1 binding partner and showed that CHL1 interacts with the first extracellular loop of PTCH1 via its extracellular domain. Co-localization and -immunoprecipitation of CHL1 with PTCH1 suggest an association of CHL1 with this major component of the hedgehog signaling pathway. The trans-interaction of CHL1 with PTCH1 promotes neuronal survival in cultures of dissociated cerebellar granule cells and of organotypic cerebellar slices...
June 19, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28615413/breaking-the-color-barrier-a-multi-selective-antibody-reporter-offers-innovative-strategies-of-fluorescence-detection
#19
Eugenio Gallo, Jonathan W Jarvik
A novel bi-partite fluorescence platform exploits the high affinity and selectivity of antibody scaffolds to capture and activate small molecule fluorogens. In this report we investigated the property of multi-selectivity activation from a single antibody against diverse cyanine-family fluorogens. Our fluorescence screen identified three cell impermeant fluorogens; each with unique emission spectra (blue, green, and red) and nanomolar affinities. Most importantly, as a protein fusion-tag to G-protein-coupled receptors the antibody biosensor retained full activity - displaying bright fluorogen signals with minimal background on live cells...
June 14, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28615412/insights-into-cortical-microtubule-nucleation-and-dynamics-in-arabidopsis-leaf-cells
#20
Noriyoshi Yagi, Sachihiro Matsunaga, Takashi Hashimoto
Plant microtubules (MTs) are nucleated from the γ-tubulin-containing ring complex (γTuRC). In cortical MT arrays of interphase plant cells, γTuRC is preferentially recruited to the lattice of preexisting MTs, where it initiates MT nucleation in either a branch- or bundle-forming manner, or dissociates without nucleation. In this study, we analyzed how γTuRCs influence MT nucleation and dynamics in cotyledon pavement cells of Arabidopsis thaliana We found that γTuRC nucleated MTs at angles of approximately 40 degrees toward the plus-ends of existing MTs, or in predominantly antiparallel bundles...
June 14, 2017: Journal of Cell Science
journal
journal
21391
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"