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Journal of Cellular Physiology

Mahdi Abbasian, Elham Mousavi, Zahra Arab-Bafrani, Amirhossein Sahebkar
Several surface markers have been proposed for the identification and characterization of colorectal cancer stem-like cells (CR-CSLCs). However, their reliability in CR-CSLCs identification remains controversial. This study evaluated the correlation between all candidate surface marker's expression and CSLCs properties (tumorigenicity) through monitoring in vivo tumor incidence and final tumor volume. PubMed, Web of Science, and Scopus databases were systematically searched until November 2017. A total of 27 studies were found that met the inclusion criteria for cluster of differentiation 133 (CD133) and CD44 markers...
October 14, 2018: Journal of Cellular Physiology
Hsiao-Chi Tsai, An-Chen Chang, Chun-Hao Tsai, Yuan-Li Huang, Lijun Gan, Chi-Kuan Chen, Shih-Chia Liu, Te-Yang Huang, Yi-Chin Fong, Chih-Hsin Tang
In recent years, osteosarcoma survival rates have failed to improve significantly with conventional treatment modalities because of the development of chemotherapeutic resistance. The human breast cancer resistance protein/ATP binding cassette subfamily G member 2 (BCRP/ABCG2), a member of the ATP-binding cassette family, uses ATP hydrolysis to expel xenobiotics and chemotherapeutics from cells. CCN family member 2 (CCN2) is a secreted protein that modulates the biological function of cancer cells, enhanced ABCG2 protein expression and activation in this study via the α6β1 integrin receptor and increased osteosarcoma cell viability...
October 14, 2018: Journal of Cellular Physiology
Kun Li, Chunmei Xiu, Qiang Zhou, Li Ni, Jun Du, Tingting Gong, Meng Li, Saijilafu, Huilin Yang, Jianquan Chen
Osteoblasts, the chief bone-forming cells, are differentiated from mesenchymal stromal/stem cells. Disruption of this differentiation process can cause osteoporosis, a bone disease characterized by low bone mass and deteriorated bone structure. Cholesterol has been implicated in pathogenesis of osteoporosis, and was recently identified as an endogenous activator of Hedgehog (Hh) signaling. However, its pathological and physiological roles in osteoblast differentiation are still poorly understood. Moreover, it is unclear whether these potential roles played by cholesterol are related to its capability to modulate Hh pathway...
October 14, 2018: Journal of Cellular Physiology
Min Zhang, Li Yan, Gui-Jie Wang, Ronghui Jin
Resistin, secreted by macrophages in tumor microenvironment, has never been investigated in pancreatic cancer models, despite a vibrant tumor microenvironment around pancreatic tumors. We evaluated serum resistin levels in healthy individuals versus pancreatic cancer patients representing different tumor grades. In vitro mechanistic analysis involved MiaPaCa-2 and SW1990 cells. Resistin signaling depends on binding of resistin to its cognitive receptors. Therefore, we silenced adenylyl cyclase-associated protein 1 (CAP1) and toll-like receptor 4 (TLR4), its two known receptors, individually as well as in combination, by short hairpin RNA (shRNA)...
October 14, 2018: Journal of Cellular Physiology
Walaa Hassan Nazmy, Eman Abdelmonem Elbassuoni, Fatma Farrag Ali, Rehab Ahmed Rifaai
Diabetes mellitus (DM) is closely associated with male infertility and sexual dysfunction. Recent data indicate that the proinsulin C-peptide (CP) exerts important physiological effects and shows the characteristics of an endogenous peptide hormone. So, this study was done to investigate the effect of C-peptide with or without insulin treatment on testicular function and architecture in diabetic rats. Rats were divided into the following groups: control, diabetic, and diabetic groups treated with either CP alone or combined with insulin...
October 14, 2018: Journal of Cellular Physiology
Xue-Liang Zhou, Yi-Hu Fang, Li Wan, Qi-Rong Xu, Huang Huang, Rong-Rong Zhu, Qi-Cai Wu, Ji-Chun Liu
PURPOSE: During myocardial infarction (MI), cardiac fibroblasts (CFs) transform into myofibroblast (CMT). This study aimed to investigate the crosstalk of Notch1 and transforming growth factor-β1 (TGF-β1)/Smad3 signaling in the regulation of CMT and myocardial fibrosis. METHODS: Primary CFs were isolated from young rats and treated with TGF-β1 or adenovirus to overexpress or knockdown Notch1 intracellular domain (N1ICD) or Smad3. RESULTS: TGF-β1 decreased the expression of fibroblast markers but increased the expression of myofibroblast markers in rat CFs...
October 14, 2018: Journal of Cellular Physiology
Paloma González-Rodríguez, Irene F Ugidos, Diego Pérez-Rodríguez, Berta Anuncibay-Soto, María Santos-Galdiano, Enrique Font-Belmonte, José Manuel Gonzalo-Orden, Arsenio Fernández-López
Brain-derived neurotrophic factor (BDNF) is considered as a putative therapeutic agent against stroke. Since BDNF role on oxidative stress is uncertain, we have studied this role in a rat brain slice ischemia model, which allows BDNF reaching the neural parenchyma. Hippocampal and cerebral cortex slices were subjected to oxygen and glucose deprivation (OGD) and then returned to normoxic conditions (reperfusion-like, RL). OGD/RL increased a number of parameters mirroring oxidative stress in the hippocampus that were reduced by the BDNF presence...
October 14, 2018: Journal of Cellular Physiology
Shupeng Shi, Mimi Tang, Honglei Li, Hui Ding, Yangfan Lu, Lijuan Gao, Qian Wu, Luo Zhou, Yujiao Fu, Bo Xiao, Mengqi Zhang
Angiogenesis is positively correlated with the survival rate of stroke patients. Therefore, studying factors that initiate and promote angiogenesis after ischemic stroke is crucial for finding novel and effective treatment targets that improve the prognosis of stroke. X-box binding protein l splicing (XBP1s) plays a positive regulatory role in cell proliferation and angiogenesis. However, the role and mechanism of XBP1s on the proliferation of brain microvascular endothelial cells (BMECs) and angiogenesis after cerebral ischemia remains unclear...
October 14, 2018: Journal of Cellular Physiology
Mohammad N Oskouie, Nazanin S Aghili Moghaddam, Alexandra E Butler, Parvin Zamani, Amirhossein Sahebkar
Curcumin, the bioactive pigment of turmeric which has polyphenolic-hydrophobic components, has been used for the treatment of a variety of diseases. However, due to its insignificant intestinal-liver metabolism, low stability, quick systemic elimination and its hydrophobic property with low solubility, curcumin has limited bioavailability. Exosomes are nanovesicles (30-100 nm) released from diverse cell types into extracellular and, ultimately, into bio-fluids in a tightly regulated manner. Exosomes are capable of transferring lipids, proteins, RNAs and DNAs, both with and without direct cell-to-cell contact...
October 14, 2018: Journal of Cellular Physiology
Filipe R Carvalho, Sofia M Calado, Gabriela A Silva, Gabriela S Diogo, Joana Moreira da Silva, Rui L Reis, M Leonor Cancela, Paulo J Gavaia
Type 1 diabetes mellitus (T1DM) has been associated to several cartilage and bone alterations including growth retardation, increased fracture risk, and bone loss. To determine the effect of long term diabetes on bone we used adult and aging Ins2 Akita mice that developed T1DM around 3-4 weeks after birth. Both Ins2 Akita and wild-type (WT) mice were analyzed at 4, 6, and 12 months to assess bone parameters such as femur length, growth plate thickness and number of mature and preapoptotic chondrocytes. In addition, bone microarchitecture of the cortical and trabecular regions was measured by microcomputed tomography and gene expression of Adamst-5, Col2, Igf1, Runx2, Acp5, and Oc was quantified by quantitative real-time polymerase chain reaction...
October 14, 2018: Journal of Cellular Physiology
Nian-Kang Sun, Abhidha Kohli, Shang-Lang Huang, Ting-Chang Chang, Chuck C-K Chao
We report here that the androgen receptor (AR) and ABCB1 are upregulated in a model of acquired taxol resistance (txr) in ovarian carcinoma cells. AR silencing sensitizes txr cells to taxol threefold, whereas ectopic AR expression in AR-null HEK293 cells induces resistance to taxol by 1.7-fold. AR activation using the agonist dihydrotestosterone (DHT) or sublethal taxol treatment upregulates ABCB1 expression in both txr cells and AR-expressing HEK293 cells. In contrast, AR inactivation using the antagonist bicalutamide downregulates ABCB1 expression and enhances cytotoxicity to taxol...
October 14, 2018: Journal of Cellular Physiology
Xiangcheng Xiao, Qiongjing Yuan, Yusa Chen, Zhihua Huang, Xi Fang, Haixia Zhang, Ling Peng, Ping Xiao
Although long noncoding RNA (LncRNA) are important players in the initiation and progression of many pathological processes, the role of LncRNAENST00000453774.1 (LncRNA 74.1) in renal fibrosis still remains unclear. Lentivirus mediated LncRNA 74.1 overexpressing HK2 cells and overexpression mice models were constructed. HK2 cells induced by transforming growth factor-β (TGF-β) in vitro, and the mice UUO model in vivo were used to simulate renal fibrosis. The expression of LncRNA 74.1 was significantly downregulated in the TGF-β-induced HK-2 cell fibrosis and clinical renal fibrosis specimens...
October 14, 2018: Journal of Cellular Physiology
Arturo Bevilacqua, Jessica Dragotto, Alessandro Giuliani, Mariano Bizzarri
Mice exposed to continuous light undergo functional and histological changes that mimic those of human Polycystic Ovary Syndrome (PCOS). We herein induced the syndrome by exposing 30-day-old females to 10 weeks of permanent light. Ovarian morphology and histology, as well as reproductive parameters (time of observed pregnancy/delivery) were investigated. Ovaries of PCOS-modeled mice showed lack of tertiary follicles and corpora lutea, altered ovarian architecture, and increased thickness of the theca layer...
October 14, 2018: Journal of Cellular Physiology
Xing-Yu Hong, Jie Lin, Wei-Wei Gu
Aberrant blood vessel functioning and systemic circulation are key causes for vascular disorders; cardiovascular, cerebrovascular, renal artery stenosis, and peripheral artery diseases. Epidemiological and basic science evidence supported genetic reasons, compounded by obesity, hypercholesterolemia, hypertension, diabetes, and smoking as risk factors. This is an umbrella review of risk factors and therapies in vascular disorders, exploring systematic reviews and meta-analyses studies in PubMed, Cochrane, Embase, and Central published in January 2000-May 2018...
October 14, 2018: Journal of Cellular Physiology
Xin-Yue Lian, Ji-Chun Ma, Jing-Dong Zhou, Ting-Juan Zhang, De-Hong Wu, Zhao-Qun Deng, Zhi-Hui Zhang, Xi-Xi Li, Pin-Fang He, Yang Yan, Jiang Lin, Jun Qian
The current study was aimed to investigate integrin beta-like 1 (ITGBL1) methylation pattern and its clinical relevance in patients with acute myeloid leukemia (AML). Real-time methylation-specific polymerase chain reaction (PCR; RQ-MSP) and bisulfite sequencing PCR (BSP) were performed to detect the methylation of ITGBL1 promoter. Real-time quantitative PCR (RT-qPCR) was performed to analyze ITGBL1 transcript level. The results showed that ITGBL1 methylation level in 131 patients with AML was significantly higher than 29 controls (p < 0...
October 14, 2018: Journal of Cellular Physiology
Majid Ahmadi, Mahnaz Ghaebi, Samaneh Abdolmohammadi-Vahid, Sanaz Abbaspour-Aghdam, Kobra Hamdi, Sedigheh Abdollahi-Fard, Shahla Danaii, Parisa Mosapour, Ladan Koushaeian, Sanam Dolati, Reza Rikhtegar, Farnaz Dabiri Oskouei, Leili Aghebati-Maleki, Mohammad Nouri, Mehdi Yousefi
BACKGROUND: Recurrent miscarriage (RM) has a multifactorial etiology mainly due to chromosomal abnormalities and immunological factors. Treating RM has remained to be a challenging issue and the role of intravenous immunoglobulin (IVIG) in treating RM is still controversial. MATERIALS AND METHODS: This study aimed to evaluate the changes in natural killer (NK) cells' frequency and cytotoxicity in patients with RM who received the IVIG therapy. A total of 78 women with a history of three or more recurrent miscarriages were included and their peripheral blood was drawn in case of positive pregnancy test...
October 14, 2018: Journal of Cellular Physiology
Lixin Zhang, Cui Ma, Xiaoying Wang, Siyu He, Qian Li, Yutian Zhou, Ying Liu, Min Zhang, Xiufeng Yu, Xijuan Zhao, Fei Li, Da-Ling Zhu
Abnormal airway smooth muscle cells (ASMCs) proliferation is an important pathological process in airway remodeling contributes to increased mortality in asthma. Mitochondrial dynamics and metabolism have a central role in the maintenance of the cell function. In this study, lipopolysaccharide (LPS)-induced ASMCs proliferative model was used to investigate the effect of mitochondria on the proliferation of ASMCs and the possible mechanism. We used cell and molecular biology to determine the effect of dynamin-related protein 1 (Drp1) on LPS-mediated ASMCs cell cycle progression and glycolysis...
October 14, 2018: Journal of Cellular Physiology
Meng Cai, Sitao Li, Yunfei Shuai, Jie Li, Jieqiong Tan, Qiyi Zeng
Tumor necrosis factor α (TNF-α), a pivotal cytokine in sepsis, protects the host against pathogens by promoting an inflammatory response while simultaneously inducing apoptosis of the vascular endothelium. Unfortunately, inhibitors targeting certain components of the TNF-α signaling pathway to reduce cellular apoptosis have failed to translate into clinical applications, partly due to the adverse effects of excessive immunosuppression. In an attempt to discover potential targets in the TNF-α signaling pathway to modulate moderate inflammation and apoptosis during the development of sepsis, we performed a pooled genome-wide CRISPR/Cas9 knockout screen in human umbilical vein endothelial cells (HUVECs)...
October 14, 2018: Journal of Cellular Physiology
Jalal Choupani, Mohammad R Alivand, Sima M Derakhshan, Mohammad Zaeifizadeh, Mahmoud S Khaniani
Distinct metastasis is one of the main causes of breast cancer (BC)-related mortality and epithelial-mesenchymal transition (EMT) is a primary step in metastasis dissemination. On the other hand, doxorubicin (DOX) is an effective chemotherapeutic agent against BC; unfortunately, its clinical use is limited by dose-dependent side effects. Therefore, extensive efforts have been dedicated to suppressing metastasis of BC and also to overcome DOX side effects together with keeping its antitumor efficacy. Studies supported the role of oleuropein (OLEU) in reducing DOX-induced side effects besides its antitumor actions...
October 14, 2018: Journal of Cellular Physiology
Amirhossein Bahreyni, Melika Rezaei, Afsane Bahrami, Majid Khazaei, Hamid Fiuji, Mikhail Ryzhikov, Gordon A Ferns, Amir Avan, Seyed Mahdi Hassanian
Aberrant microRNA (miR) expression is implicated in multiple human malignancies. miR-21, acting as a proto-oncogene, is involved in a variety of cellular processes and tumorigenesis and is frequently overexpressed in some cancer types. Several tumor suppressors, metastatic, and apoptotic genes have been identified as miR-21 targets, including Ras homolog gene family member B, PTEN, Sprouty2, programmed cell death 4, Integrin-β4, and E-cadherin thereby regulating tumor growth, invasion, and metastasis. There is a growing evidence that miR-21 expression is associated with clinical outcomes in patients with colorectal cancer (CRC)...
October 14, 2018: Journal of Cellular Physiology
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