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Journal of Cellular Physiology

Tahereh Bakhtiari, Afshin Ghaderi, Sayyed Reza Safaee Nodehi, Zahra Aghazadeh, Farzaneh Tofighi Zavareh, Fahimeh Jafarnezhad-Ansariha, Anis Barati, Abbas Mirshafiey
β-d-Mannuronic acid (M2000), a novel non-steroidal anti-inflammatory drug (NSAID) with immunosuppressive properties, has been previously shown to exhibit potential therapeutic effects on autoimmune diseases. Immunosuppression therapy has been a standard approach for myelodysplastic syndrome (MDS) for many years. We evaluated the effect of M2000 on isolated peripheral blood mononuclear cells (PBMCs) from patients with MDS. The PBMCs were isolated from 13 patients with MDS and 13 normal donors. The cells were then treated with low, moderate, and high doses of M2000 and diclofenac as a control group...
December 10, 2018: Journal of Cellular Physiology
Rasoul Abdollahzadeh, Abdolreza Daraei, Yaser Mansoori, Masoumeh Sepahvand, Mahsa M Amoli, Javad Tavakkoly-Bazzaz
Breast cancer (BC) is the most frequently occurring malignancy in women worldwide. Despite the substantial advancement in understanding the molecular mechanisms and management of BC, it remains the leading cause of cancer death in women. One of the main reasons for this obstacle is that we have not been able to find the Achilles heel for the BC as a highly heterogeneous disease. Accumulating evidence has revealed that noncoding RNAs (ncRNAs), play key roles in the development of BC; however, the involving of complex regulatory interactions between the different varieties of ncRNAs in the development of this cancer has been poorly understood...
December 10, 2018: Journal of Cellular Physiology
Xuan Yang, Jia-Bin Cai, Rui Peng, Chuan-Yuan Wei, Jia-Cheng Lu, Chao Gao, Zhao-Zuo Shen, Peng-Fei Zhang, Xiao-Yong Huang, Ai-Wu Ke, Guo-Ming Shi, Jia Fan
Hepatocellular carcinoma (HCC) is one of the most fatal cancers with common features of invasion and metastasis. Recent evidence indicate that the long noncoding RNA NORAD is a potential oncogene and is significantly upregulated in several cancers. However, the general biological role and clinical value of NORAD in HCC remains unknown. Here, NORAD expression was measured in 29 paired tumor and paratumor tissues via quantitative real-time polymerase chain reaction (qPCR). The effects of NORAD on HCC cell malignant potential were investigated via NORAD overexpression and knockdown both in vitro and in vivo...
December 10, 2018: Journal of Cellular Physiology
Xiaofan Chen, Rongdi Yuan, Ling Gao, Chanjuan Huang, Wei Fan, Jian Ye, Chunlin Chen
OBJECTIVE: To study the mechanism of collapsin response mediator protein-2 (CRMP-2) phosphorylation changes and cyclin-dependent kinase 5 (CDK5) expression after optic nerve injury. METHODS: Optic nerve injury rat models were constructed, the messenger RNA (mRNA) level of CRMP-2 in optic nerve tissues was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) after building models 0, 3, 7, and 14 days. The protein expression of CRMP-2, phospho-CRMP-2 (p-CRMP-2), and CDK5 were also determined by western blot analysis...
December 10, 2018: Journal of Cellular Physiology
Yanwei Yin, Lei Du, Xuezhen Li, Xiaoyan Zhang, Yongli Gao
OBJECTIVE: This study aimed to investigate the expression levels of miR-133a-3p and collagen type I α 1 (COL1A1) in esophageal squamous cell carcinoma (ESCC) to find out the relationship between miR-133a-3p and COL1A1 and their influence on ESCC propagation, migration, invasion, and apoptosis. METHODS: The messenger RNA expression levels of miR-133a-3p and COL1A1 in ESCC were detected by quantitative reverse-transcription polymerase chain reaction. The expression of COL1A1 protein was examined via western blot analysis and immunohistochemistry assay...
December 10, 2018: Journal of Cellular Physiology
Kebing Zhou, Pingbo Yao, Jun He, Hong Zhao
Lipid autophagy (lipophagy) is defined as a selective autophagy process in which some intracellular lipid droplets are selectively degraded by autophagic lysosomes pathway. The occurrence of lipophagy was first discovered in liver tissues. Additionally, abundant evidence indicated that the occurrence of hepatic lipophagy has been implicated in many liver diseases including fatty liver diseases, nonalcoholic fatty liver diseases, liver fibrosis, and liver cirrhosis. However, recent studies suggested that hepatic lipophagy occurs not only in liver tissue but also in other nonliver tissues and cells...
December 10, 2018: Journal of Cellular Physiology
Farzaneh Ghafarian, Mehran Pashirzad, Majid Khazaei, Majid Rezayi, Seyed Mahdi Hassanian, Gordon A Ferns, Amir Avan
Cardiovascular disease (CVD) is the major cause of death globally; therefore, there is a need for the identification of a valid biomarker that accurately predicts the risk of developing CVD, and novel therapeutic approaches for its treatment. Exosomes are very small extracellular vesicles containing protein, lipid, transcription factors, messenger RNAs, noncoding RNA, and nucleic acid contents that are important players in intercellular communication, and that act via long-range signals or cell-to-cell contact...
December 10, 2018: Journal of Cellular Physiology
Yaron Ilan
Microtubules (MT) and actin microfilaments are dynamic cytoskeleton components involved in a range of intracellular processes. MTs play a role in cell division, beating of cilia and flagella, and intracellular transport. Over the past decades, much knowledge has been gained regarding MT function and structure, and its role in underlying disease progression. This makes MT potential therapeutic targets for various disorders. Disturbances in MT and their associated proteins are the underlying cause of diseases such as Alzheimer's disease, cancer, and several genetic diseases...
December 10, 2018: Journal of Cellular Physiology
Elaheh Esmaeili, Mahsa Khalili, Alireza Naderi Sohi, Simzar Hosseinzadeh, Behnaz Taheri, Masoud Soleimani
Magnetic iron oxide nanoparticles are a well-explored class of nanomaterials known for their high magnetization and biocompatibility. They have been used in various biomedical applications such as drug delivery, biosensors, hyperthermia, and magnetic resonance imaging (MRI) contrast agent. It is necessary to surface modify the nanoparticles with a biocompatible moiety to prevent their agglomeration and enable them to target to the defined area. Dendrimers have attracted considerable attention due to their small size, monodispersed, well-defined globular shape, and a relative ease incorporation of targeting ligands...
December 10, 2018: Journal of Cellular Physiology
Qiong Xie, Zipu Yu, Yuan Lu, Jingya Fan, Yiming Ni, Liang Ma
Son of sevenless (SOS) is one of the guanine nucleotide exchange factors that can regulate the mitogen-activated protein kinase/extracellular signal regulated kinase signal pathway via controlling the activation of Ras. microRNAs are key regulon of gene expression and would be treated as tumor biomarkers or therapeutic targets. In this study, we find that miR-148a-3p acts as a tumor-suppressor in the development and progression of non-small-cell lung cancer (NSCLC). miR-148a-3p inhibits NSCLC cells proliferation and epithelial-mesenchymal transition by reducing the expression of SOS2, which refers Ras activating...
December 10, 2018: Journal of Cellular Physiology
Ying Zhang, Longhui Ma, Changhong Ren, Kaiyin Liu, Xin Tian, Di Wu, Yuchuan Ding, Junfa Li, Cesar V Borlongan, Xunming Ji
Remote ischemic postconditioning (RIPC) is a promising neuroprotective strategy for ischemic stroke. Here, we employed a focal ischemic stroke mouse model to test the hypothesis that poststroke collateral circulation as a potent mechanism of action underlying the therapeutic effects of immediate RIPC. During reperfusion of cerebral ischemia, the mice were randomly assigned to receive RIPC, granulocyte colony-stimulating factor (G-CSF) as a positive control, or no treatment. At 24 hr, we found RIPC and G-CSF increased monocytes/macrophages in the dorsal brain surface and in the spleen, coupled with enhanced leptomeningeal collateral flow compared with nontreatment group...
December 10, 2018: Journal of Cellular Physiology
Fang Wang, Aihong Mao, Jinzhou Tang, Qianjing Zhang, Junfang Yan, Yupei Wang, Cuixia Di, Lu Gan, Chao Sun, Hong Zhang
Prostate cancer (CaP) is the second most common cancer in men worldwide in 2012, and radiation therapy is one of the most common definitive treatment options for localized CaP. However, radioresistance is a major challenge for the current radiotherapy, accumulating evidences suggest microRNAs (miRNAs), as an important regulator in cellular ionizing radiation (IR) responses, are closely correlated with radiosensitivity in many cancers. Here, we identified microRNA-16-5p(miR-16-5p) is significantly upregulated in CaP LNCaP cells following IR and can enhance radiosensitivity through modulating Cyclin D1/E1-pRb-E2F1 pathway...
December 10, 2018: Journal of Cellular Physiology
Laura Pizzuti, Antonio Giordano, Andrea Michelotti, Marco Mazzotta, Clara Natoli, Teresa Gamucci, Claudia De Angelis, Elisabetta Landucci, Lucrezia Diodati, Laura Iezzi, Lucia Mentuccia, Agnese Fabbri, Maddalena Barba, Giuseppe Sanguineti, Paolo Marchetti, Silverio Tomao, Luciano Mariani, Ida Paris, Vito Lorusso, Simona Vallarelli, Alessandra Cassano, Francesca Airoldi, Armando Orlandi, Luca Moscetti, Domenico Sergi, Maria Giuseppina Sarobba, Giuseppe Tonini, Daniele Santini, Valentina Sini, Enzo Veltri, Angela Vaccaro, Laura Ferrari, Michele De Tursi, Nicola Tinari, Antonino Grassadonia, Filippo Greco, Andrea Botticelli, Nicla La Verde, Claudio Zamagni, Daniela Rubino, Enrico Cortesi, Valentina Magri, Giulia Pomati, Simone Scagnoli, Elisabetta Capomolla, Ramy Kayal, Angelo Fedele Scinto, Domenico Corsi, Marina Cazzaniga, Lucio Laudadio, Samantha Forciniti, Maria Mancini, Luisa Carbognin, Patrizia Seminara, Sandro Barni, Riccardo Samaritani, Mario Roselli, Ilaria Portarena, Antonio Russo, Corrado Ficorella, Katia Cannita, Silvia Carpano, Mirco Pistelli, Rossana Berardi, Ruggero De Maria, Isabella Sperduti, Gennaro Ciliberto, Patrizia Vici
Data from 423 human epidermal growth factor receptor 2-negative (HER2-), hormone receptor-positive (HR+) advanced breast cancer (aBC) patients treated with palbociclib and endocrine therapy (ET) were provided by 35 Italian cancer centers and analyzed for treatment outcomes. Overall, 158 patients were treated in first line and 265 in second/later lines. We observed 19 complete responses and 112 partial responses. The overall response rate (ORR) was 31% (95% confidence interval [CI], 26.6-35.4) and clinical benefit was 52...
December 10, 2018: Journal of Cellular Physiology
Romana Šínová, Jana Kudová, Kristina Nešporová, Sergej Karel, Romana Šuláková, Vladimír Velebný, Lukáš Kubala
The stimulation of myocardium repair is restricted due to the limited understanding of heart regeneration. Interestingly, endogenous opioid peptides such as dynorphins and enkephalins are suggested to support this process. However, the mechanism-whether through the stimulation of the regenerative capacity of cardiac stem cells or through effects on other cell types in the heart-is still not completely understood. Thus, a model of the spontaneous cardiomyogenic differentiation of mouse embryonic stem (mES) cells via the formation of embryoid bodies was used to describe changes in the expression and localization of opioid receptors within cells during the differentiation process and the potential of the selected opioid peptides, dynorphin A and B, and methionin-enkephalins and leucin-enkephalins, to modulate cardiomyogenic differentiation in vitro...
December 10, 2018: Journal of Cellular Physiology
Samantha Milanesi, Daniela Verzola, Francesca Cappadona, Barbara Bonino, Abitha Murugavel, Roberto Pontremoli, Giacomo Garibotto, Francesca Viazzi
Renal proximal tubular cells (PTECs) participate in several mechanisms of innate immunity, express toll-like receptors (TLRs), and proinflammatory cytokines. Hyperuricemia may be a promoter of inflammation and renal damage. Angiotensin II (Ang II) modulate immune and inflammatory responses in renal tubular cells. With the aim to evaluate the effect of uric acid (UA) and Ang II on oxidative stress and inflammation mediated by toll-like receptor 4 (TLR4) activation in human PTECs, human kidney 2 (HK2) were incubated for 24 hr with UA (12 mg/dl) and Ang II (10 -7  M)...
December 10, 2018: Journal of Cellular Physiology
Haidong He, Ping Hu, Yuyan Tang, Xudong Xu
OBJECTIVE: This study aimed to explore the underlying function of Gubenxiezhuo dialysis on the distribution of gut microflora uremia. METHODS: A uremia rat model was constructed, and the morphology of renal tissue was determined using the hematoxylin-eosin (H&E) staining. Moreover, the blood samples were collected and the expression of IL-1β, IL-6, and CRP was determined using enzyme-linked immunosorbent assay. Following these experiments, the gut tissues of rats were collected and the distribution of gut microbiota was explored using real-time PCR...
December 10, 2018: Journal of Cellular Physiology
Homa Nomani, Golnaz Bayat, Amirhossein Sahebkar, Amir Farjam Fazelifar, Farveh Vakilian, Vahid Jomezade, Thomas P Johnston, Amir Hooshang Mohammadpour
Cardiac complications including arrhythmia and especially atrial fibrillation (AF) are common causes of death in β-thalassemia patients. The main factor in the etiopathogenesis of these complications is iron overload, which results in increased oxidative stress. Although there is a known association between cardiac complications and iron overload in β-thalassemia patients, there is no comprehensive review on AF and excessive iron with a focus on oxidative stress in these patients. The aim of this article was to review the different aspects of AF in β-thalassemia patients with a focus on the prevention and treatment of AF by using iron chelators and/or anti-oxidants...
December 10, 2018: Journal of Cellular Physiology
Xiumei Chen, Jianzhong Lin, Tingting Hu, Zhiyun Ren, Linnan Li, Irbaz Hameed, Xiaoyu Zhang, Chen Men, Yan Guo, Di Xu, Yiyang Zhan
Oxidized low-density lipoprotein (Ox-LDL)-induced endothelial cell injury plays a crucial role in the pathogenesis of atherosclerosis (AS). Plasma galectin-3 (Gal-3) is elevated inside and drives diverse systemic inflammatory disorders, including cardiovascular diseases. However, the exact role of Gal-3 in ox-LDL-mediated endothelial injury remains unclear. This study explores the effects of Gal-3 on ox-LDL-induced endothelial dysfunction and the underlying molecular mechanisms. In this study, Gal-3, integrin β1, and GTP-RhoA in the blood and plaques of AS patients were examined by ELISA and western blot respectively...
December 10, 2018: Journal of Cellular Physiology
Fatemeh Ghanaatian, Naser-Aldin Lashgari, Amir Hossein Abdolghaffari, Seyed M Rajaee, Yunes Panahi, George E Barreto, Alexandra E Butler, Amirhossein Sahebkar
Multiple sclerosis (MS) is a disease that has shown a considerable increase in prevalence in recent centuries. Current knowledge about its etiology is incomplete, and therefore it cannot be managed optimally utilizing targeted therapeutic regimens at each stage of the disease. MS progresses in different stages, beginning with a cascade of inflammation. The pivotal spark to initiate this cascade seems to be the migration of Th17 into the central nervous system across the blood-brain barrier (BBB) through the disrupted tight junctions...
December 10, 2018: Journal of Cellular Physiology
Hossein Nikzad, Hamed Sabzalipoor, Javad Amini Mahabadi, Elahe Seyedhosseini, Seyed Ehsan Enderami, Seyed Mohammad Gheibi Hayat, Amirhosein Sahebkar
New perspectives have been opened by advances in stem cell research for reproductive and regenerative medicine. Several different cell types can be differentiated from stem cells (SCs) under suitable in vitro and in vivo conditions. The differentiation of SCs into male germ cells has been reported by many groups. Due to their unlimited pluripotency and self-renewal, embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) can be used as valuable tools for drug delivery, disease modeling, developmental studies, and cell-based therapies in regenerative medicine...
December 10, 2018: Journal of Cellular Physiology
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