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Cryobiology

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https://www.readbyqxmd.com/read/28807662/a-preliminary-study-of-osmotic-dehydration-in-zebrafish-embryos-implications-for-vitrification-and-ultra-fast-laser-warming
#1
Michelle H Connolly, Estefania Paredes, Peter Mazur
To date, traditional cryopreservation techniques have not been amenable to zebrafish (Danio rerio) embryos, due in part to their large yolky eggs, which have a low surface to volume ratio and limited permeability to water and cryoprotectants. However, recent vitrification and ultra-rapid warming studies in mice have demonstrated successful preservation by dehydrating 85% or more of their total water content. We hypothesized that this approach may help overcome the barriers to embryo cryopresrvation among D...
August 11, 2017: Cryobiology
https://www.readbyqxmd.com/read/28803846/embryo-survival-and-birth-after-minimum-volume-vitrification-or-slow-freezing-of-in-vivo-and-in-vitro-produced-ovine-embryos
#2
P C Dos Santos-Neto, F Cuadro, N Barrera, M Crispo, A Menchaca
The objective was to evaluate pregnancy outcomes and birth rate of in vivo derived vs. in vitro produced ovine embryos submitted to different cryopreservation methods. A total of 197 in vivo and 240 in vitro produced embryos were cryopreserved either by conventional freezing, or by vitrification with Cryotop or Spatula MVD methods on Day 6 after insemination/fertilization. After thawing/warming and transfer, embryo survival rate on Day 30 of gestation was affected by the source of the embryos (in vivo 53.3%, in vitro 20...
August 10, 2017: Cryobiology
https://www.readbyqxmd.com/read/28803845/improvement-of-post-thaw-sperm-survivals-using-liquid-nitrogen-vapor-in-a-spermcasting-oyster-ostrea-angasi
#3
Md Mahbubul Hassan, Xiaoxu Li, Jian G Qin
Low survival of cryopreserved sperm impedes the application of cryopreservation technique in spermcasting oyster species. This study developed a simple method of liquid nitrogen vapor freezing to improve post-thaw sperm survival in the spermcasting oyster Ostrea angasi. The results indicate that the permeable cryoprotectants, dimethyl sulfoxide (DMSO), ethylene glycol (EG) and propylene glycol (PG) were non-toxic to sperm up to 20% concentration and 90 min exposure whereas methanol at 10% or higher was toxic to sperm for any exposure over 30 min...
August 10, 2017: Cryobiology
https://www.readbyqxmd.com/read/28782503/cryopreservation-of-rat-hepatocytes-with-disaccharides-for-cell-therapy
#4
Liana Monteiro da Fonseca Cardoso, Marcelo Alves Pinto, Andrea Henriques Pons, Luiz Anastácio Alves
Cryopreservation of hepatocytes is a crucial step in the implementation of cell therapy for treating certain liver diseases. In the present study we investigated the effect of the some disaccharides on the cryopreservation of rat hepatocytes. Liver cells were frozen in media in the presence or absence of low concentrations of Me2SO (5% Me2SO) supplemented with varying concentrations of disaccharides (sucrose, glucose and trehalose). After 7 days of cryopreservation, the hepatocytes were thawed and viability was measure by exclusion of trypan blue and by the MTT technique, as well as by determining albumin production...
August 4, 2017: Cryobiology
https://www.readbyqxmd.com/read/28782504/cryoelectrolysis-an-acute-case-study-in-the-pig-liver
#5
Franco Lugnani, Enric Gunther, Pedro Torrecillas, Carlos Galacho, Adolfo Jiménez Garrido, Paul Mikus, Nina Klein, Michael K Stehling, Matteo Macchioro, Liel Rubinsky, Narayan Raju, Boris Rubinsky
We report results from an acute, single case study in the pig liver on the effects of a tissue ablation protocol (we named cryoelectrolysis) in which 10 min of cryosurgery, with a commercial cryosurgical probe, are delivered after 10 min of electrolysis generated by a current of about 60 mA. The histological appearance of tissue treated with cryoelectrolysis is compared with the appearance of tissue treated with 10 min of cryosurgery alone and with 10 min of electrolysis alone. Histology done after 3 h survival shows that the mixed rim of live and dead cells found around the ablated lesion in both cryosurgery and electrolytic ablation is replaced by a sharp margin between life and dead cells in cryoelectrolysis...
August 3, 2017: Cryobiology
https://www.readbyqxmd.com/read/28778690/comparative-analysis-of-the-transcriptome-of-the-overwintering-desert-beetle-microdera-punctipennis
#6
Kuerban Tusong, Xiaoxing Guo, Shanshan Meng, Xiaoning Liu, Ji Ma
The cold tolerance mechanisms of insect have been studied extensively on the model species Drosophila and a few other species at the transcriptional level. However studies on insects that inherit strong cold tolerance are limited. Cold hardy Tenebrionid beetle Microdera punctipennis is endemic to Gurbantonggut Desert, northwest of China. However, its genomic information is lacking. To investigate the overwintering mechanisms of M. punctipennis adult, RNA-seq was performed on the winter adults and the control adults that were kept in laboratory at 30 °C...
August 1, 2017: Cryobiology
https://www.readbyqxmd.com/read/28774548/safety-and-efficiency-of-oocyte-vitrification
#7
REVIEW
Neelke De Munck, Gábor Vajta
As the oocyte is the starting point for a new life, artificial reproductive technology (ART) techniques should not affect the (ultra) structural and functional integrity, or the developmental competence. Oocyte vitrification -one of the most significant achievements in human ART during the past decade- should therefore be a safe and efficient technique. This review discusses the principles and developments of the existing and future techniques, applications possibilities and safety concerns. The broad range of vitrification media and devices that are currently available, show differences in their effects on the oocyte ultrastructure and preimplantation development...
August 1, 2017: Cryobiology
https://www.readbyqxmd.com/read/28757053/cryopreservation-of-indian-red-jungle-fowl-gallus-gallus-murghi-semen-with-polyvinylpyrrolidone
#8
Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, Zartasha Zafar, Iftikhar Hussain, Julian Santiago-Moreno, Elisabeth Blesbois
The Indian red jungle fowl is a sub-species of the genus Gallus native to South Asia; facing high risks of extinction in its native habitat. During cryopreservation, permeable cryoprotectants like glycerol are usually employed and we previously showed encouraging results with 20% glycerol. Because bird spermatozoa contain very little intracellular water, the possibility of replacing an internal cryoprotectant by an external one is opened. In the present study, we tested the replacement of internal cryoprotectant glycerol by the external cryoprotectant Polyvinylpyrrolidone (PVP)...
July 27, 2017: Cryobiology
https://www.readbyqxmd.com/read/28754211/survival-of-a-cardiac-arrested-victim-with-hypothermia-despite-severely-elevated-serum-potassium-9-0-mmol-l
#9
LETTER
Mathieu Pasquier, Tomasz Darocha, Paul Husby
No abstract text is available yet for this article.
July 25, 2017: Cryobiology
https://www.readbyqxmd.com/read/28743515/the-influence-of-systemic-cryotherapy-on-selected-hemodynamic-parameters-and-the-assessment-of-the-safety-of-its-use-in-patients-with-successfully-treated-hypertension
#10
Ewa Wujek-Krajewska, Agnieszka Cudnoch-Jędrzejewska, Liana Puchalska, Barbara Chybowska, Maciej Janiszewski, Dominika Klimczak, Marek Kuch
No abstract text is available yet for this article.
July 22, 2017: Cryobiology
https://www.readbyqxmd.com/read/28716599/maintaining-viability-and-characteristics-of-cholangiocarcinoma-tissue-by-vitrification-based-cryopreservation
#11
Min Zeng, Qiu-Rui Yang, Gong-Bo Fu, Yuan Zhang, Xu Zhou, Wei-Jian Huang, Hong-Dan Zhang, Wei-Jian Li, Zhen-Yu Wang, He-Xin Yan, Bo Zhai
Tumor tissue has great clinical and scientific value which relies highly on the proper preservation of primary materials. Conventional tumor tissue cryopreservation using slow-freezing method has yielded limited success, leading to significant cell loss and morphological damage. Here we report a standardized vitrification-based cryopreservation method, by which we have successfully vitrified and warmed 35 intrahepatic cholangiocarcinoma (ICC) tissues with up to 80% viability of the fresh tumor tissues. Cryopreserved ICC tissue could generate patient-derived xenografts (PDXs) with take rates of 68...
July 14, 2017: Cryobiology
https://www.readbyqxmd.com/read/28716598/effect-of-lecithin-nanoliposome-or-soybean-lecithin-supplemented-by-pomegranate-extract-on-post-thaw-flow-cytometric-microscopic-and-oxidative-parameters-in-ram-semen
#12
Mahdieh Mehdipour, Hossein Daghigh Kia, Maryam Nazari, Abouzar Najafi
This investigation was carried out to study the effect of soybean lecithin 1.5% (wt/vol) (0, 2.5, 5 and 7.5 mg l(-1) pomegranate extract (PE)) or PE-loaded lecithin nanoliposome (0, 2.5, 5 and 7.5 mg l(-1)) to Tris-based extender. Sperm motility (CASA), viability, membrane integrity (HOS test), abnormalities, mitochondrial activity, apoptosis status, lipid peroxidation, total antioxidant capacity (TAC)) and antioxidant activities (GPX, SOD) were investigated following freeze-thawing. No significant differences (P < 0...
July 14, 2017: Cryobiology
https://www.readbyqxmd.com/read/28716597/cells-isolated-from-cryopreserved-dental-follicle-display-similar-characteristics-to-cryopreserved-dental-follicle-cells
#13
Hefeng Yang, Jie Li, JingJing Sun, Weihua Guo, Hui Li, Jinglong Chen, Yu Hu, Weidong Tian, Song Li
Dental follicle tissue is a promising resource of mesenchymal stem cells for cytotherapeutic approaches and tissue engineering applications. There are two procedures for banking of human dental follicle stem cells have been reported. Conventional method requires cell isolation, expansion and immediate cryopreservation. Whereas dental follicle stem cells can be isolated from cryopreserved dental follicle fragments. The aim of this study was to compare the characteristics of dental follicle cells isolated from cryopreserved fragments (DFCs-CF) with dental follicle cells recovered from cryopreserved cells (DFCs-CC)...
July 14, 2017: Cryobiology
https://www.readbyqxmd.com/read/28716596/quality-comparison-of-umbilical-cord-blood-cryopreserved-with-conventional-versus-automated-systems
#14
Kyeong Mi Kim, Ji Young Huh, Jin Ju Kim, Myung Seo Kang
Umbilical cord blood (CB) banks usually freeze and store CB for clinical transplantation using conventional controlled-rate freezer or the automated BioArchive system. The aim of this study is to compare the quality of CB cryopreserved with conventional and automated methods and to make clear the cause of the quality difference between the two methods. The experiment used 80 CB units: 40 were conventionally cryopreserved and the remainder were cryopreserved with a BioArchive. After thawing, the following measures of CB quality were compared: recovery rates of cell count, cell viability of total nucleated cells (TNCs), mononuclear cells (MNCs), and CD34+ cells, as well as colony-forming unit-granulocyte/macrophage (CFU-GM) content...
July 14, 2017: Cryobiology
https://www.readbyqxmd.com/read/28697988/the-addition-of-reduced-glutathione-to-cryopreservation-media-induces-changes-in-the-structure-of-motile-subpopulations-of-frozen-thawed-boar-sperm
#15
Efrén Estrada, Maria M Rivera Del Álamo, Joan E Rodríguez-Gil, Marc Yeste
Adding cryopreservation media with reduced glutathione (GSH) has previously been shown to maintain the motility, membrane integrity and fertilizing ability of frozen-thawed boar sperm, although the effects of GSH on good (GFE) and poor freezability (PFE) ejaculates rely upon the intrinsic ejaculate freezability. The resilience to withstand freeze-thawing procedures has previously been related to the existence of a specific distribution of motile sperm subpopulations, which differs between GFE and PFE. Thus, the main aim of this study was to determine whether the addition of GSH to freezing media has any impact on the distribution of motile sperm subpopulations in GFE and PFE...
July 8, 2017: Cryobiology
https://www.readbyqxmd.com/read/28693953/cryopreservation-of-luciola-praeusta-kiesenwetter-coleoptera-lampyridae-embryos-by-vitrification
#16
Kang Li, Xuanli Chen, Xiaoling Song, Xi Wu, Yi Xian
There is an urgent need to preserve the ever-decreasing number of different species of fireflies all over the world. We sought to develop a vitrification procedure to cryopreserve the firefly embryos. The late stages of Luciola praeusta Kiesenwetter embryos were collected. Several impermeable and permeable protectants with various concentrations in different mediums (TNM-FH insect medium, Grace's medium, Dulbecco's Modification of Eagle's Medium (DMEM) and Dulbecco's Phosphate-Buffered Saline (DPBS)) was used...
July 7, 2017: Cryobiology
https://www.readbyqxmd.com/read/28668447/effect-of-cryopreservation-on-viability-and-growth-efficiency-of-stromal-epithelial-cells-derived-from-neonatal-human-thymus
#17
Valentin P Shichkin, Oleksandr I Gorbach, Olga A Zuieva, Nataliia I Grabchenko, Irina A Aksyonova, Boris M Todurov
The thymus is the major site of T lymphocyte generation and so is critical for a functional adaptive immune system. Since, thymectomy is a component of neonatal surgery for congenital heart diseases, it provides great potential for collection and storage of thymic tissue for autologous transplantation. However, specific investigation into the optimum parameters for thymic tissue cryopreservation have not been conducted. In this research, we evaluated the effect of different cryoprotective media compositions, which included penetrating (Me2SO, glycerol) and non-penetrating (dextran-40, sucrose, hydroxyethyl starch) components, on the viability and functionality of frozen-thawed human thymic samples to select an optimal cryoprotective medium suitable for long-term storage of thymic tissue and a stromal-epithelial enriched population...
June 28, 2017: Cryobiology
https://www.readbyqxmd.com/read/28645681/thermal-thresholds-of-cardiovascular-hl-1-cell-destruction-by-cryothermal-exposure
#18
Jeunghwan Choi, John Bischof
The use of thermal based therapies for treatment of atrial fibrillation is increasing [2,9]. While numerous reports are available in the literature regarding the efficacy of cryotherapy on pulmonary vein survival, there are no reports specifically at the cellular level that establish thermal thresholds and mechanisms of cellular destruction. The current article reports on the response of HL-1 cardiomyocytes to cooling rates and end temperatures during cryothermal exposure. The focus is on establishment of in vitro thresholds while also establishing mechanisms of action due to biophysical events (i...
June 20, 2017: Cryobiology
https://www.readbyqxmd.com/read/28645680/collection-processing-and-freezing-of-equine-bone-marrow-cells
#19
Fernanda Z Bastos, Fernanda C M Barussi, Thasla F Santi, Bianca P Vieira, Alexandra C Senegaglia, Fernanda F Cruz, Pedro V Michelotto
There is no consensus on aspects of equine bone marrow collection and processing. The study aimed to describe the collection of large volumes of bone marrow from horses of advanced age, with emphasis on bone marrow mononuclear cells (BMMCs) recovery and viability after cryopreservation. Fourteen horses, aged 3-24 years, were divided into three experiments. E1 studied the feasibility of collecting 200 mL from the sternums of horses of advanced age; E2 examined the number of cells obtained from the first and last syringe of each puncture; and E3 investigated the influence of heparin concentration on the prevention of cell aggregation, and cell viability after freezing in liquid nitrogen...
June 20, 2017: Cryobiology
https://www.readbyqxmd.com/read/28641947/effectiveness-of-human-spermatozoa-biomarkers-as-indicators-of-structural-damage-during-cryopreservation
#20
María José Gómez-Torres, Llanos Medrano, Alejandro Romero, Pedro José Fernández-Colom, Jon Aizpurúa
Human spermatozoa cryopreservation techniques are used to maintain and protect male fertility in cases such as infertility and malignancy treatments. However, during cryopreservation, the spermatozoa's metabolic rate is reduced and they undergo dramatic functional and structural changes owing to exposure to cryoprotectants and freezing-thawing procedures. While the effects of cryopreservation on cells are documented, to date the induced cryodamage on structural and/or functional sperm biomarkers is not well established at multivariate scale...
June 19, 2017: Cryobiology
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