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Cryobiology

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https://www.readbyqxmd.com/read/29458044/cryoprotective-effect-of-antifreeze-polyamino-acid-carboxylated-poly-l-lysine-on-bovine-sperm-a-technical-note
#1
Takuro Fujikawa, Shuji Imamura, Motoyuki Tokumaru, Takaaki Ando, Yuki Gen, Suong-Hyu Hyon, Chikara Kubota
In this study, investigating Carboxylated Poly-L-Lysine (CPLL) for its effectiveness as a new cryoprotectant for bovine sperm is aimed. CPLL is an ampholytic polymer compound, has cryoprotective properties similar to those of anti-freeze protein. The cryopreservation medium used for control group consisted of 6.5% (v/v) glycerol, the cryopreservation medium used for experimental group consisted of 3.25% (v/v) glycerol + 0.5% (w/v) CPLL. There was no consequential difference in sperm motility parameter after thawing whereas there was huge distinction for sperm membrane integrity rate (control vs experimental; 49...
February 16, 2018: Cryobiology
https://www.readbyqxmd.com/read/29458043/optimization-of-a-protective-medium-for-enhancing-the-viability-of-freeze-dried-bacillus-amyloliquefaciens-b1408-based-on-response-surface-methodology
#2
Lingjuan Han, Taixun Pu, Xi Wang, Beiling Liu, Yonghong Wang, Juntao Feng, Xing Zhang
Response surface methodology (RSM) is a commonly used system to optimize cryoprotectants of biocontrol strains when they are subjected to preparations. Various kinds of cryoprotectants and centrifugal conditions were tested to improve the survival of biocontrol agents after freeze-drying. To determine the optimum levels of incorporation of three cryoprotectants (glucose, trehalose and xylitol) in the freeze-drying process of strain Bacillus amyloliquefaciens B1408, a range of experiments based on Box-Behnken Design (BBD) were conducted...
February 16, 2018: Cryobiology
https://www.readbyqxmd.com/read/29458042/pretreatment-with-cholesterol-loaded-cyclodextrins-prevents-loss-of-motility-associated-proteins-during-cryopreservation-of-addra-gazelle-nanger-dama-ruficollis-spermatozoa
#3
Jessye Wojtusik, Yan Wang, Budhan S Pukazhenthi
Sperm cryopreservation is challenging, often resulting in irreversible damage to spermatozoa, as indicated by decreased motility, viability, and/or acrosomal integrity. Developing cryopreservation protocols for gametes of endangered species compounds the complexity of technique optimization; samples are difficult to obtain and numbers are limited. Cryopreservation of sperm collected from the critically endangered addra gazelle (Nanger dama ruficollis), a member of the Bovidae family, resulted in significant loss of motility, which was prevented by pretreatment with cholesterol-loaded cyclodextrin (CLC)...
February 16, 2018: Cryobiology
https://www.readbyqxmd.com/read/29458041/role-of-superoxide-ion-formation-in-hypothermia-rewarming-induced-contractile-dysfunction-in-cardiomyocytes
#4
Niccole Schaible, Young Soo Han, Torkjel Tveita, Gary C Sieck
Rewarming following accidental hypothermia is associated with circulatory collapse due primarily to impaired cardiac contractile (systolic) function. Previously, we found that reduced myofilament Ca2+ sensitivity underlies hypothermia/rewarming (H/R)-induced cardiac contractile dysfunction. This reduced Ca2+ sensitivity is associated with troponin I (cTnI) phosphorylation. We hypothesize that H/R induces reactive oxygen species (ROS) formation in cardiomyocytes, which leads to cTnI phosphorylation and reduced myofilament Ca2+ sensitivity...
February 16, 2018: Cryobiology
https://www.readbyqxmd.com/read/29452078/ice-free-cryopreservation-of-heart-valve-tissue-the-effect-of-adding-mitoq-to-a-vs83-formulation-and-its-influence-on-mitochondrial-dynamics
#5
Yulong Sui, Qing Fan, Bin Wang, Jixian Wang, Qing Chang
No abstract text is available yet for this article.
February 13, 2018: Cryobiology
https://www.readbyqxmd.com/read/29448018/applications-of-gray-level-variation-detection-method-to-intracellular-ice-formation
#6
Yabo Wang, Kai Zhu, Xiaochuan Zhang, Hongwei Ji
Intracellular ice formation (IIF) is the major cause of death in cells subjected to freezing. The occurrence of intracellular ice prevents the penetration of light into the camera and makes the image dark. Therefore, the gray-level variation can reflect the IIF. However, cell deformation is accompanied with IIF, especially for larger cells. It is necessary to account this entire phenomenon together in a single method. In this paper, the normalized parameter C defined by the gray-level variation depending on the displacement was defined to reflect the gray-level change of each pixel point in the region of interest of the image...
February 12, 2018: Cryobiology
https://www.readbyqxmd.com/read/29448017/fetal-bovine-serum-free-cryopreservation-methods-for-clinical-banking-of-human-adipose-derived-stem-cells
#7
Seah Park, Dong Ryul Lee, Ji Sun Nam, Chul Woo Ahn, Haekwon Kim
The use of fetal bovine serum (FBS) as a cryopreservation supplement is not suitable for the banking of mesenchymal stem cells (MSCs) due to the risk of transmission of disease as well as xenogeneic immune reactions in the transplanted host. Here, we investigated if human serum albumin (HSA), human serum (HS), or knockout serum replacement (KSR) can replace FBS for the cryopreservation of MSCs. In addition, we examined the characteristics of MSCs after multiple rounds of cryopreservation. Human adipose-derived stem cells (ASCs) cryopreserved with three FBS replacements, 9% HSA, 90% HS, or 90% KSR, in combination with 10% dimethyl sulfoxide (Me 2 SO) maintained stem cell properties including growth, immunophenotypes, gene expression patterns, and the potential to differentiate into adipogenic, osteogenic, and chondrogenic lineages, similar to ASCs frozen with FBS...
February 12, 2018: Cryobiology
https://www.readbyqxmd.com/read/29432715/attenuation-of-cryopreservation-induced-oxidative-stress-by-antioxidant-impact-of-coenzyme-q10-on-the-quality-of-post-thawed-buck-spermatozoa
#8
Iman Yousefian, Mojtaba Emamverdi, Amir Karamzadeh-Dehaghani, Raziyeh Sabzian-Melei, Mahdi Zhandi, Ahmad Zare-Shahneh
The aim of this study was to determine the quality of post-thawed buck spermatozoa by attenuation of cryopreservation-induced oxidative stress using CoQ10, a lipophilic antioxidant. Ejaculates at every sampling period were collected from four Mahabadi bucks, pooled and diluted in soybean lecithin-based extenders containing 0 (negative control, NC), 0.5 (CQ0.05), 1 (CQ1), and 1.5 (CQ1.5) μM CoQ10 and 0.9% (v/v) DMSO (positive control, PC). The diluted semen was gradually cooled to 4 °C, then frozen and stored in liquid nitrogen...
February 9, 2018: Cryobiology
https://www.readbyqxmd.com/read/29428531/vitrification-of-one-cell-mouse-embryos-in-cryotubes
#9
Shinsuke Seki, Keita Basaki, Yukie Komatsu, Yasuyoshi Fukuda, Megumi Yano, Yuhei Matsuo, Takahiro Obata, Yukihisa Matsuda, Kazutoshi Nishijima
Preventing intracellular ice formation is essential to cryopreserve cells. Prevention can be achieved by converting cell water into a non-crystalline glass, that is, to vitrify. The prevailing belief is that to achieve vitrification, cells must be suspended in a solution containing a high concentration of glass-inducing solutes and cooled rapidly. In this study, we vitrified 1-cell mouse embryos and examined the effect of the cooling rate, the warming rate, and the concentration of cryoprotectant on cell survival...
February 8, 2018: Cryobiology
https://www.readbyqxmd.com/read/29427573/cryoprotective-role-of-organic-zn-and-cu-supplementation-in-goats-capra-hircus-diet
#10
Arunachalam Arangasamy, Mayasula Venkata Krishnaiah, Narasimhaiah Manohar, Sellappan Selvaraju, Guvvala Pushpa Rani, Nira Manik Soren, Ippala Janardhan Reddy, Janivara Parameshwaraiah Ravindra
The current study focused on cryopreservation and assessment of characters of post-thaw semen of indigenous Osmanabadi bucks maintained with standard diet, supplemented with different concentrations of organic zinc (Zn), copper (Cu) or in combination, for a period of 180 days. The different doses of organic Zn and Cu were fed per kg DM basis, Zn groups (low: Zn20, medium: Zn40 and high: Zn60, Cu groups: (low: Cu12.5, medium: Cu25 and high: Cu37.5 and combination of Zn + Cu groups (low: Zn20 + Cu12.5, medium: Zn40 + Cu25 and high: Zn60 + Cu37...
February 7, 2018: Cryobiology
https://www.readbyqxmd.com/read/29408447/cryopreservation-effects-on-sperm-function-and-fertility-in-two-threatened-crane-species
#11
Megan E Brown, Ram P Singh, Budhan Pukazhenthi, Carol L Keefer, Nucharin Songsasen
The capacity to cryopreserve semen from captive cranes facilitates production of offspring from behaviorally incompatible or geographically separated pairs, and allows for long-term preservation of valuable genetic materials. The present study sought to develop effective cryopreservation protocols for whooping (Grus americana) and white-naped (Grus vipio) cranes, through examining the influences of two permeating (DMA and Me2SO) and one non-permeating (sucrose) cryoprotectants, as well as vitamin E on post-thaw sperm survival...
February 3, 2018: Cryobiology
https://www.readbyqxmd.com/read/29397923/improved-cryosurvival-of-stallion-spermatozoa-after-colloid-centrifugation-is-independent-of-the-addition-of-seminal-plasma
#12
Essraa M Al-Essawe, Anders Johannisson, Manuela Wulf, Christine Aurich, Jane M Morrell
Addition of seminal plasma (SP) prior to cryopreservation may influence stallion sperm cryosurvival. The objective of this study was to investigate the addition of pooled SP from "good" or "bad" freezer stallions to spermatozoa selected by single layer centrifugation (SLC) prior to cryopreservation on post-thaw sperm quality. Semen from 12 stallions was collected; 5 mL was frozen as control (C) and the remainder was processed by SLC to remove SP and was divided into three aliquots: i) SLC sample without SP (SLC); ii) SLC plus pooled SP from "good freezer" stallions (SLC-GF); iii) SLC plus pooled SP from "bad freezer" stallions (SLC-BF)...
February 1, 2018: Cryobiology
https://www.readbyqxmd.com/read/29397921/high-capacity-ice-recrystallization-endpoint-assay-employing-superhydrophobic-coatings-that-is-equivalent-to-the-splat-assay
#13
Laurie A Graham, Prashant Agrawal, Richard D Oleschuk, Peter L Davies
We have developed an ice recrystallization inhibition (IRI) assay system that allows the side-by-side comparison of up to a dozen samples treated in an identical manner. This system is ideal for determining, by serial dilution, the IRI 'endpoint' where the concentration of a sample is reached that can no longer inhibit recrystallization. Samples can be an order of magnitude smaller in volume (<1 μL) than those used for the conventional 'splat' assay. The samples are pipetted into wells cut out of a superhydrophobic coating on sapphire slides that are covered with a second slide and then snap-frozen in liquid nitrogen...
February 1, 2018: Cryobiology
https://www.readbyqxmd.com/read/29397922/the-effects-of-different-preservation-methods-on-ide-leuciscus-idus-sperm-and-the-longevity-of-sperm-movement
#14
G Bernáth, Zs Csenki, Z Bokor, L Várkonyi, J Molnár, T Szabó, Á Staszny, Á Ferincz, K Szabó, B Urbányi, L O Pap, B Csorbai
The present study investigated the effects of chilled storage and cryopreservation on ide sperm motility and fertilizing capacity alongside the longevity of sperm movement. The parameters of motility (progressive motility-pMOT, curvilinear velocity-VCL and straightness-STR) have been recorded during 48 h of chilled storage (4 °C) at 24-h intervals. The longevity of sperm movement was measured following activation for up to 120 s (in a range at 10-120 s) in freshly stripped and thawed sperm. A formerly established cryopreservation method was tested on ide sperm where motility parameters, hatching rate and larval malformation (according to 7 category groups) were investigated...
January 31, 2018: Cryobiology
https://www.readbyqxmd.com/read/29407225/chilling-causes-perivitelline-granule-formation-in-activated-zebrafish-oocytes
#15
Fatemeh Jalali, Angela J DiBenedetto, Jens O M Karlsson
Chilling sensitivity in oocytes of the zebrafish represents a potential obstacle to their successful cryopreservation. Here, we report the first cryomicroscopic observations of the response of zebrafish oocytes to chilling conditions. In activated stage V oocytes that had been exposed to hypothermic temperatures, we observed a latent effect of chilling, manifesting as a granular precipitate that appeared in the perivitelline fluid upon return to 28.5 °C. The granules were visible in unstained oocytes under transmitted light microscopy, and the resulting perivitelline turbidity increased in a dose-dependent manner with decreasing chilling temperature (p < 0...
January 30, 2018: Cryobiology
https://www.readbyqxmd.com/read/29366764/deglycerolization-of-red-blood-cells-a-new-dilution-filtration-system
#16
Jing Liu, Weiping Ding, Xiaoming Zhou, Yufeng Kang, Lili Zou, Chengpan Li, Xingxia Zhu, Dayong Gao
In this work, we present a new version of the dilution-filtration system for rapidly deglycerolizing a large volume of cryopreserved blood. In our earlier system, one of the major problems was the damage induced to the red blood cells (RBCs) due to high osmolality change at the dilution point. Therefore, we devised a new system to solve this problem. First, we theoretically simulated the osmolality variation in the new system and the variation of the maximum and minimum volumes of the RBCs at the dilution point to examine the effects of operating parameters/conditions...
January 20, 2018: Cryobiology
https://www.readbyqxmd.com/read/29366763/long-term-preservation-of-freeze-dried-rabbit-sperm-by-adding-rosmarinic-acid-and-different-chelating-agents
#17
Paula Domingo, Maite Olaciregui, Noelia González, Ignacio De Blas, Lydia Gil
Freeze-drying (FD) technique has been applied as an alternative technology to preserve gene resources to allow simple sperm preservation and shipment at 4 °C. Nevertheless, DNA sperm might be damaged by mechanical or oxidative stress throughout FD procedure. Therefore, suitable protection to maintain DNA integrity is required. The aim of this study was to determine the effect of rosmarinic acid (RA) as an antioxidant and two chelating agents (EGTA and EDTA) on the DNA integrity of freeze-dried rabbit sperm after storage of the samples at 4 °C and room temperature for 8 months...
January 20, 2018: Cryobiology
https://www.readbyqxmd.com/read/29355519/successful-sperm-cryopreservation-of-the-brown-marbled-grouper-epinephelus-fuscoguttatus-using-propylene-glycol-as-cryoprotectant
#18
Maisarah Yusoff, Mohd Badrul Nizam Hassan, Muhammad Ikhwanuddin, Shahreza Md Sheriff, Fatimah Hashim, Sufian Mustafa, Ivan Chong Chu Koh
This study developed the cryopreservation of brown-marbled grouper spermatozoa for practical application. We examined 32 cryodiluents, developed from four types of cryoprotectants [propylene glycol (PG), dimethyl-sulphoxide (Me2SO), dimethyl-acetamide (DMA) and ethylene glycol (EG)] at four concentrations of 5, 10, 15 and 20% in combination with two extenders [Fetal bovine serum (FBS) and artificial seminal plasma (ASP). Cooling rates were examined by adjusting the height of straws (2.5-12.5 cm) from the liquid nitrogen (LN) vapour and cooled for 5 min before immersion into LN...
January 18, 2018: Cryobiology
https://www.readbyqxmd.com/read/29352980/effect-of-two-cooling-protocols-on-the-post-thaw-characteristics-of-iberian-ibex-sperms
#19
Milagros C Esteso, Adolfo Toledano-Díaz, Cristina Castaño, Jorgea Pradiee, Antonio Lopez-Sebastián, Julián Santiago-Moreno
The rate at which lethal intracellular ice forms during sperm cryopreservation is highly dependent on the cooling protocol. The present work compares two cooling protocols for use with Iberian ibex (Capra pyrenaica) sperm by assessing the effects on the motility, viability, and size of frozen-thawed sperm cells. Ejaculates, obtained from six adult ibex males via transrectal, ultrasound-guided massage of the accessory sex glands plus electroejaculation if necessary, were cooled via either 1) Protocol 1 (decelerating cooling), involving cooling in liquid nitrogen vapor from 5 °C to -35 °C (40 °C/min), from -35 °C to -65 °C (17 °C/min), and then from -65 °C to -85 °C (3 °C/min); or 2) Protocol 2 (accelerating cooling) involving cooling in a biological freezer from 5 °C to -5 °C (4 °C/min), from -5 °C to -110 °C (25 °C/min), and then from -110 °C to -140 °C (35 °C/min)...
January 15, 2018: Cryobiology
https://www.readbyqxmd.com/read/29352979/evaluation-of-sheep-ovarian-tissue-cryopreserved-by-slow-freezing-or-vitrificaton-after-chick-embryo-chorioallantoic-membrane-transplantation
#20
Mahboubeh Vatanparast, Mohammad Ali Khalili, Nahid Yari, Marjan Omidi, Mehdi Mohsenzadeh
The aim of our investigations was to compare the effectiveness of two methods for cryopreservation of sheep ovarian tissue, slow freezing and vitrification. The quality of cryopreserved tissues was evaluated after 5 days of thawing and chorioallantoic membrane (CAM) transplantation. Follicular structure, stromal integrity and neovascularization were assessed. The areas of fibrosis and necrosis were measured using MICROVISIBLE software, and proliferation was assessed with Ki-67 immunostaning. After 5 days of culture, the proportion of primordial follicles decreased, whereas the primary and intermediary follicles increased insignificantly (p > ...
January 15, 2018: Cryobiology
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