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Cryobiology

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https://www.readbyqxmd.com/read/30312592/optimization-of-protocols-for-microinjection-based-delivery-of-cryoprotective-agents-into-japanese-whiting-sillago-japonica-embryos
#1
Md Ariful Alam, Sheikh Mustafizur Rahman, Yoji Yamamoto, Ricardo Shohei Hattori, Toru Suzuki, Manabu Watanabe, Carlos Augusto Strüssmann
Microinjection has proven useful for introduction of low-permeability cryoprotective agents (CPAs) into fish eggs or embryos for cryopreservation. In this work, we examined the suitable conditions for single or combined microinjection into the perivitelline space (PS) and the yolk mass (YM) of embryos of the Japanese whiting, an alternative marine fish model for embryo cryopreservation studies. The parameters examined were injection volume, CPA type and concentration, vehicle (diluent), and suitable developmental stage...
October 9, 2018: Cryobiology
https://www.readbyqxmd.com/read/30312591/thermal-expansion-of-substrate-may-affect-adhesion-of-chinese-hamster-fibroblasts-to-surfaces-during-freezing
#2
Taylor Rutt, Nasim Eskandari, Maria Zhurova, Janet A W Elliott, Locksley E McGann, Jason P Acker, John A Nychka
Despite success in cryopreservation of cells in suspension, cryopreservation of cells in monolayers is still challenging. One of the major problems is detachment of the cells from the substrate which occurs during cryopreservation. We hypothesized that this detachment may be due to a mismatch in the coefficient of linear thermal expansion (αL ) between glass and the frozen cell layer which manifests as residual stress and stress relaxation. This mismatch results in a difference between the thermal expansion of ice and glass as they undergo temperature changes...
October 9, 2018: Cryobiology
https://www.readbyqxmd.com/read/30296411/properties-of-phase-transition-of-ice-binding-protein-from-arctic-yeast-leibp-utilizing-differential-scanning-calorimetry-dsc-and-raman-spectroscopy
#3
Sanghwa Lee, Jun Hyuck Lee, Han-Woo Kim, Jong Wook Hong
Ice binding proteins (IBPs) have been attracting significant interest on account of their characteristic of inhibiting ice growth and recrystallization. Owing to their unique characteristics, IBPs have been studied for applications in food, pharmaceuticals, and medicine, as well as from a general scientific point of view. In this study, we have used differential scanning calorimetry (DSC) and Raman spectroscopy as tools to understand the ice binding activity of the Arctic-yeast-originating extracellular ice binding glycoprotein (LeIBP) isolated from Leucosporidium sp...
October 5, 2018: Cryobiology
https://www.readbyqxmd.com/read/30296410/proteome-variation-of-the-rat-liver-after-static-cold-storage-assayed-in-an-ex-vivo-model
#4
Camila Knecht, Cecilia L Balaban, Joaquín V Rodríguez, Eduardo A Ceccarelli, Edgardo E Guibert, Germán L Rosano
Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associated with injury during cold storage and/or normothermic reperfusion using the isolated perfused rat liver model. Livers from male rats were subjected to either (1) cold storage for 24 h, (2) ex vivo normothermic reperfusion for 90 min or (3) cold storage for 24 h followed by ex vivo normothermic reperfusion for 90 min...
October 5, 2018: Cryobiology
https://www.readbyqxmd.com/read/30292812/hypothermic-pulsatile-perfusion-of-human-pancreas-preliminary-technical-feasibility-study-based-on-histology
#5
J Branchereau, K Renaudin, Delphine Kervella, S Bernadet, Georges Karam, Gilles Blancho, D Cantarovich
BACKGROUND: There are currently two approaches to hypothermic preservation for most solid organs: static or dynamic. Cold storage is the main method used for static storage (SS), while hypothermic pulsatile perfusion (HPP) and other machine perfusion-based methods, such as normothermic machine perfusion and oxygen persufflation, are the methods used for dynamic preservation. HPP is currently approved for kidney transplantation. METHODS: We evaluated, for the first time, the feasibility of HPP on 11 human pancreases contraindicated for clinical transplantation because of advanced age and/or history of severe alcoholism and/or abnormal laboratory tests...
October 4, 2018: Cryobiology
https://www.readbyqxmd.com/read/30292811/cryopreservation-of-human-umbilical-vein-and-porcine-corneal-endothelial-cell-monolayers
#6
Nasim Eskandari, Leah A Marquez-Curtis, Locksley E McGann, Janet A W Elliott
Cryopreservation of endothelium is one of the major challenges in the cryopreservation of complex tissues. Human umbilical vein endothelial cells (HUVECs) in suspension are available commercially and recently their post-thaw cell membrane integrity was significantly improved by cryopreservation in 5% dimethyl sulfoxide (Me2 SO) and 6% hydroxyethyl starch (HES). However, cryopreservation of cells in monolayers has been elusive. The exact mechanisms of damage during cell monolayer cryopreservation are still under investigation...
October 4, 2018: Cryobiology
https://www.readbyqxmd.com/read/30292810/post-rapid-freezing-growth-of-antarctic-strain-of-heterococcus-sp-monitored-by-cell-viability-and-chlorophyll-fluorescence
#7
Alla Orekhova, Miloš Barták, Josef Hájek
The soil microalgae of the genus Heterococcus are found in cold environments and have been reported for the terrestrial ecosystems of several Sub-Antarctic and Antarctic Islands. This study focused on resistance of Heterococcus sp. to sub-zero temperature. Heterococcus sp. was isolated from soil samples from James Ross Island, Antarctica. Culture of Heterococcus sp. grown in liquid medium were used to study ribitol effects at sub-zero temperatures on the species resistance to rapid freezing (RF, immersion of a sample into liquid nitrogen) and consequent cultivation on agar...
October 4, 2018: Cryobiology
https://www.readbyqxmd.com/read/30266383/impact-of-vitrification-on-granulosa-cell-survival-and-gene-expression
#8
Maria Kokotsaki, Mario Mairhofer, Christian Schneeberger, Julian Marschalek, Detlef Pietrowski
INTRODUCTION: Cryopreservation of ovarian tissue is an essential step in Ovarian Tissue Banking. In order to prevent the formation of ice crystals, typically the tissue is slowly frozen using a cryoprotectant. As an alternative the method of ultra-fast freezing by vitrification becomes more attention for freezing ovarian tissue because it has successfully been used for oocytes, embryos and sperm. However the impact of vitrification on granulosa cells, which are an essential part of ovarian tissue is uncertain...
September 25, 2018: Cryobiology
https://www.readbyqxmd.com/read/30257179/changes-of-soluble-sugars-and-atp-content-during-dmso-droplet-freezing-and-pvs3-droplet-vitrification-of-potato-shoot-tips
#9
Claudia Köpnick, Marion Grübe, Johanna Stock, Angelika Senula, Hans-Peter Mock, Manuela Nagel
The potato's great genetic diversity needs to be maintained for future agricultural applications and can be preserved at ultra-low temperatures. To decipher detailed physiological processes, the aim of the study was to analyze the regrowth in 28 gene bank accessions and to reveal metabolite changes in a subset of four accessions that showed pronounced differences after shoot tip cryopreservation using DMSO droplet freezing and PVS3 droplet vitrification. Regrowth varied in all 28 genotypes ranging from 5% ('Kagiri') to 100% ('Karakter') and was higher after PVS3 droplet vitrification (71 ± 19%) than after cryopreservation using DMSO (54 ± 17%)...
September 23, 2018: Cryobiology
https://www.readbyqxmd.com/read/30219374/the-application-of-convolution-neural-network-based-cell-segmentation-during-cryopreservation
#10
Momoh Karmah Mbogba, Zeeshan Haider, S M Chapal Hossain, Daobin Huang, Kashan Memon, Fazil Panhwar, Zeling Lei, Gang Zhao
For most of the cells, water permeability and plasma membrane properties play a vital role in the optimal protocol for successful cryopreservation. Measuring the water permeability of cells during subzero temperature is essential. So far, there is no perfect segmentation technique to be used for the image processing task on subzero temperature accurately. The ice formation and variable background during freezing posed a significant challenge for most of the conventional segmentation algorithms. Thus, a robust and accurate segmentation approach that can accurately extract cells from extracellular ice that surrounding the cell boundary is needed...
September 13, 2018: Cryobiology
https://www.readbyqxmd.com/read/30218644/faster-cheaper-defined-and-efficient-vitrification-for-immature-porcine-oocytes-through-modification-of-exposure-time-macromolecule-source-and-temperature
#11
Ruth Appeltant, Tamás Somfai, Kazuhiro Kikuchi
Vitrification reduces the developmental competence of porcine immature oocytes. We investigated the effects of modifying various factors on the viability and development of oocytes after vitrification. These factors included: 1) exposure to the vitrification solution, 2) macromolecule addition (bovine serum albumin (BSA) or polyvinyl pyrrolidone (PVP)), 3) treatment with cytochalasin B, 4) equilibration temperature, and 5) vitrification method (microdrop or Cryotop). Oocytes were equilibrated and vitrified using medium containing ethylene glycol and propylene glycol...
September 13, 2018: Cryobiology
https://www.readbyqxmd.com/read/30165074/cryopreservation-of-plagiognathops-microlepis-sperm
#12
Chen Du, Ying-Li Han, Ying-Xue Shi, Jun-Quan Zhu
Sperm was collected from cultured male fish and cryopreserved in 0.25 mL straws for the study of sperm cryopreservation. Different parameters were evaluated, including extender, dilution ratio, cryoprotectant type and concentration, equilibrium time, cooling height (in a two-step cooling protocol), and thawing temperature. The optimum result was obtained when the sperm was diluted at a 1:7 ratio in D-16 with 5% DMSO as a cryoprotectant, equilibrated for 20 min, held at 3 cm above liquid nitrogen for 10 min, and then stored in liquid nitrogen...
August 27, 2018: Cryobiology
https://www.readbyqxmd.com/read/30148986/construction-of-human-artificial-ovary-from-cryopreserved-ovarian-tissue-appearance-of-apoptosis-and-necrosis-after-enzymatic-isolation-of-follicles
#13
Viola Maria Schmidt, Evgenia Isachenko, Gunter Rappl, Gohar Rahimi, Bettina Hanstein, Bernd Morgenstern, Peter Mallmann, Vladimir Isachenko
Earlier it was shown that number of retrieved follicles was significantly higher in Tumor Dissociation Enzyme (TDE)-treatment group compare to standard Liberase TM-group. The aim of our present investigations was to examine the effect of TDE on appearance of apoptosis and necrosis in follicles and stromal cells after digesting of cryopreserved ovarian cortex. Fresh and frozen ovarian cortex fragments (OCF) from 14 patients (29 ± 6 years old), sized 20-210 mm3 were randomly distributed into four treatment groups and digested with 16% TDE or 0...
August 24, 2018: Cryobiology
https://www.readbyqxmd.com/read/30144407/role-of-synthetic-antifreeze-agents-in-catalyzing-ice-nucleation
#14
Lindong Weng, Anisa Swei, Mehmet Toner
Nature endows antifreeze (glyco)proteins (AF(G)Ps) with the excellent capability of inhibiting ice crystal growth. Recent years have also witnessed the emergence of many potent AF(G)P mimics such as poly (vinyl alcohol) (PVA). As researchers are revealing the molecular mechanisms of inhibiting ice crystal growth by AF(G)Ps and their synthetic substitutes, there remains no agreement about their effect on ice nucleation. In this study, we report the observation of ice nucleation catalyzed by PVA of different polymerization degrees using a freeze-on-a-chip platform which allows the monitoring of freezing and melting events over hundreds of monodisperse, picoliter-sized aqueous droplets...
August 22, 2018: Cryobiology
https://www.readbyqxmd.com/read/30125538/cryosurvival-after-exposure-of-ivf-derived-nellore-embryos-to-different-cryoprotectants-and-exposure-times-during-vitrification
#15
Juliano F Souza, Landry L Lienou, Ana P R Rodrigues, E Alexandrino, Tania V Cavalcante, Regiane R Santos, Jose R Figueiredo, Francisca Elda F Dias
We evaluated the effects of the vitrification solution (i.e., ethylene glycol (EG) + dimethyl sulfoxide (DMSO) with or without propylene glycol (PG)) and of exposure time on the re-expansion and hatching rates of vitrified Bos indicus embryos. In vitro produced embryos (n = 1050) were divided into seven groups: control group (non-vitrified embryos) and six vitrification groups with different cryoprotectant concentrations and exposure times. After vitrification, embryos were cultured for determination of re-expansion and hatching rates...
August 18, 2018: Cryobiology
https://www.readbyqxmd.com/read/30125537/dietary-fish-oil-supplemented-with-vitamin-e-improves-quality-indicators-of-rooster-cold-stored-semen-through-reducing-lipid-peroxidation
#16
Reza Safari Asl, Farid Shariatmadari, Mohsen Sharafi, Mohammad Amir Karimi Torshizi, Abdolhossein Shahverdi
Cockerel semen is sensitive to cooling, which limits chilled storage of semen for more than 24 h. Results of artificial insemination with cold-stored semen are not desirable. This study was conducted to evaluate the effects of dietary fish oil and vitamin E (vitE) for cold-storage of rooster semen and its effects on parameters of semen during 48 h cooling preservation. Roosters were assigned into four dietary treatments; 1) control group received a basal diet, 2) vitE group received a basal diet supplemented with 200 mg/kg vitE, 3) fish oil group (FO) received a basal diet supplemented with 2% fish oil and 4) fish oil and vitE group received a basal diet supplemented with 2% fish oil and 200 mg/kg vitE (FO + vitE)...
August 18, 2018: Cryobiology
https://www.readbyqxmd.com/read/30114383/convective-heat-transfer-coefficients-of-open-and-closed-cryotop-%C3%A2-systems-under-different-warming-conditions
#17
M V Santos, M Sansinena, J Chirife, N Zaritzky
The warming of cryopreserved samples supported by small volume devices is governed by heat transfer phenomena which are mathematically described by the solution of the transient heat conduction partial differential equations; the convective heat transfer coefficient (h) is an important parameter involved in the boundary condition which is related to the fluid dynamic behavior at the interface device-warming fluid (water, sucrose solution or air). Unfortunately, h values for small volume devices (i.e. Cryotop® ) have not been experimentally determined...
August 14, 2018: Cryobiology
https://www.readbyqxmd.com/read/30098997/effect-of-sericin-supplementation-on-heat-shock-protein-70-hsp70-expression-redox-status-and-post-thaw-semen-quality-in-goat
#18
Vidya Sagar Reddy, Brijesh Yadav, Chhote Lal Yadav, Mukul Anand, Dilip Kumar Swain, Devendra Kumar, Deepika Kritania, Arun Kumar Madan, Jitender Kumar, Sarvajeet Yadav
Cryopreservation results in substantial deterioration of heat shock protein 70 (HSP70) and ultra-structural changes in sperm organelles, resulting in a marked reduction in post-thaw semen quality. The present study was aimed to explicate the effect of sericin supplementation on expression profile of HSP70, redox status and post-thaw semen quality in Barbari goat. Five Barbari bucks were used to collect thirty semen ejaculates by using artificial vagina and each ejaculate was divided into three aliquots to which sericin was supplemented at 0% (Control), 0...
August 10, 2018: Cryobiology
https://www.readbyqxmd.com/read/30098996/single-blastocyst-transfer-set-and-pregnancy-outcome-of-day-5-and-day-6-human-blastocysts-vitrified-using-a-closed-device
#19
Romualdo Sciorio, K J Thong, Susan J Pickering
This study investigates the utility of the Rapid-i closed device for vitrification of human blastocysts on day-5 (D5) and day-6 (D6) of development and the implantation and pregnancy rate following single blastocyst transfer (SBT) of warmed D5/D6 blastocysts. This retrospective cohort study was performed at Edinburgh Assisted Conception Programme, EFREC, Royal Infirmary of Edinburgh between January 2013 and January 2017. Good quality blastocysts were vitrified on D5 or D6 using Irvine Vitrification medium (Irvine Scientific-USA) and the Rapid-I closed Vitrification System™ (Vitrolife, Sweden)...
August 10, 2018: Cryobiology
https://www.readbyqxmd.com/read/30098995/lycopene-and-alpha-lipoic-acid-improve-semen-antioxidant-enzymes-activity-and-cashmere-goat-sperm-function-after-cryopreservation
#20
Fa Ren, Tianyu Feng, Guichao Dai, Yanhua Wang, Haijing Zhu, Jianhong Hu
Cashmere goats, a unique biological resource in China, have the highest cashmere yield and best fiber quality in the world. The goal of this study was to determine the effects of cryopreserving with lycopene (LP) and alpha-lipoic acid (ALA) on the physiological characteristics of Cashmere goat spermatozoa. The results showed that sperm motility, acrosome integrity, membrane integrity, and mitochondrial activity of goat spermatozoa were greater in extenders containing 1.0 mg/mL LP and 10 μg/mL ALA (P < 0...
August 10, 2018: Cryobiology
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