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Cryobiology

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https://www.readbyqxmd.com/read/28341134/metabolic-changes-in-droplet-vitrified-semen-of-wild-endangered-persian-sturgeon-acipenser-persicus-borodin-1997
#1
Amirreza Abed-Elmdoust, Hamid Farahmand, Bagher Mojazi-Amiri, Gholamreza Rafiee, Ruhollah Rahimi
Comparative quantitative metabolite profiling can be used for better understanding of cell functions and dysfunctions in particular circumstances such as sperm banking which is an important approach for cryopreservation of endangered species. Cryopreservation techniques have some deleterious effects on spermatozoa which put the obtained results in controversy. Therefore, in the present study, quantitative (1)H NMR (Nuclear Magnetic Resonance) based metabolite profiling was conducted to evaluate metabolite changes related to energetics and some other detected metabolites in vitrified semen of critically endangered wild Acipenser persicus...
March 21, 2017: Cryobiology
https://www.readbyqxmd.com/read/28341133/sperm-cryopreservation-in-the-spermcasting-australian-flat-oyster-ostrea-angasi-by-a-programmable-freezing-method
#2
Md Mahbubul Hassan, Xiaoxu Li, Yibing Liu, Jian G Qin
Cryopreservation offers long-term storage of gametes without constraint from seasonal gamete maturation, provides opportunities to improve the efficiency of breeding and genetic programs, and protects endangered species from extinction due to epidemic diseases and natural disasters. In this study, a protocol for cryopreserving sperm of the spermcasting Australian flat oyster Ostrea angasi was developed by optimizing key factors influencing the quality of cryopreserved sperm. Dimethyl sulfoxide (DMSO) was non-toxic to sperm within the concentration and duration assessed in the toxicity experiment whereas 10% methanol or a higher concentration was toxic to sperm from the exposure duration of 30 min onwards...
March 21, 2017: Cryobiology
https://www.readbyqxmd.com/read/28315320/controlled-ice-nucleation-using-freeze-dried-pseudomonas-syringae-encapsulated-in-alginate-beads
#3
Lindong Weng, Shannon N Tessier, Anisa Swei, Shannon L Stott, Mehmet Toner
The control of ice nucleation is of fundamental significance in many process technologies related to food and pharmaceutical science and cryobiology. Mechanical perturbation, electromagnetic fields and ice-nucleating agents (INAs) have been known to induce ice nucleation in a controlled manner. But these ice-nucleating methods may suffer from cumbersome manual operations, safety concerns of external fields, and biocompatibility and recovery issues of INA particles, especially when used in living systems. Given the automatic ice-seeding nature of INAs, a promising solution to overcome some of the above limitations is to engineer a biocomposite that accommodates the INA particles but minimizes their interactions with biologics, as well as enabling the recovery of used particles...
March 14, 2017: Cryobiology
https://www.readbyqxmd.com/read/28302378/hypothermic-storage-of-isolated-spermatogonia-and-oogonia-from-rainbow-trout-oncorhynchus-mykiss
#4
Bahram Falahatkar, Samaneh Poursaeid, Ryota Kitada, Goro Yoshizaki
A growing number of fish species are endangered due to human activities. A short- or long-time preservation of gametes could conserve genetic resources of threatened fish species. The aim of this study was to evaluate a hypothermic condition for short-term preservation of spermatogonia and oogonia cells isolated from immature transgenic rainbow trout, Oncorhynchus mykiss, and to determine the maximum time point for further transplantation. Viability rate of germ cells was investigated after isolation and during storage at 4 °C up to 24 h...
March 13, 2017: Cryobiology
https://www.readbyqxmd.com/read/28288795/suppression-of-transforming-growth-factor-beta1-expression-in-keloids-after-cryosurgery
#5
Sara M Awad, Sahar A Ismail, Doaa S Sayed, Abeer E Refaiy, Rania Makboul
The biological mechanism underlying cryosurgical treatment of keloids remains unclear. Transforming growth factor-beta1 (TGF-β1) has been implicated in the pathogenesis of keloids and was reported to be the target of several therapeutic modalities. However, the effect of cryosurgery on its expression in keloid tissue has not been yet investigated. In this study, 26 consecutive keloid patients were treated with cryosurgery for 2-6 sessions. Keloids were biopsied before starting cryosurgery and after two treatment sessions for the immunohistochemical evaluation of TGF-β1 expression...
March 10, 2017: Cryobiology
https://www.readbyqxmd.com/read/28284665/cryopreservation-and-multipotential-characteristics-evaluation-of-a-novel-type-of-mesenchymal-stem-cells-derived-from-small-tailed-han-sheep-fetal-lung-tissue
#6
Caiyun Ma, Changqing Liu, Xiangchen Li, Taofeng Lu, Chunyu Bai, Yanan Fan, Weijun Guan, Yu Guo
Lung mesenchymal stem cells (L-MSCs) characterized by plasticity, reduced relative immune privilege and high anti-fibrosis characteristics play the crucial role in lung tissue regenerative processes. However, up to date, the multi-differentiation potentials and application values of L-MSCs are still uncertain. In the current study, the Small Tailed Han Sheep embryo L-MSCs line from 12 samples, stocking 124 cryogenically-preserved vials, was successfully established by using primary culture and cell cryopreservation techniques...
March 8, 2017: Cryobiology
https://www.readbyqxmd.com/read/28283337/vitrification-of-porcine-immature-oocytes-association-of-equilibration-manners-with-warming-procedures-and-permeating-cryoprotectants-effects-under-two-temperatures
#7
Guoquan Wu, Baoyu Jia, Guobo Quan, Decai Xiang, Bin Zhang, Qingyong Shao, Qionghua Hong
The aim of this study was to evaluate the association of equilibration manners with warming procedures, and the different permeating cryoprotectants (pCPAs) effects under two temperatures, in terms of survival, maturation and subsequent parthenogenetic development of porcine immature oocytes after Cryotop vitrification. In Experiment 1, oocytes were equilibrated by exposure to 5% (v/v) ethylene glycol (EG) for 10 min (EM1) or stepwise to 7.5% (v/v) and 15% (v/v) EG for 2.5 min respectively (EM2). Warming procedures were performed in 1...
March 7, 2017: Cryobiology
https://www.readbyqxmd.com/read/28279680/antioxidant-effect-of-quercetin-in-an-extender-containing-dma-or-glycerol-on-freezing-capacity-of-goat-semen
#8
Afshin Seifi-Jamadi, Ejaz Ahmad, Mahdi Ansari, Hamid Kohram
This study was performed to evaluate the effectiveness of quercetin as a non-enzymatic antioxidant in combination with glycerol or Dimethylacetamide (DMA), on freezability of goat semen. Ejaculates from four healthy mature Mahabadi goats were collected using an artificial vagina. After primary processing, semen was pooled and extended by egg yolk based extender supplemented with different concentrations of quercetin (10 or 20 μM) along with 5% glycerol or DMA. The extended semen was frozen and sperm motility parameters, viability, abnormality, membrane integrity and lipid peroxidation were assessed after thawing...
March 6, 2017: Cryobiology
https://www.readbyqxmd.com/read/28257856/the-role-of-global-histone-post-translational-modifications-during-mammalian-hibernation
#9
Shannon N Tessier, Bryan E Luu, Jeffrey C Smith, Kenneth B Storey
Mammalian hibernators must cope with hypothermia, ischemia-reperfusion, and finite fuel reserves during days or weeks of continuous torpor. One means of lowering ATP demands during hibernation involves substantial transcriptional controls. The present research analyzed epigenetic regulatory factors as a means of achieving transcriptional control over cycles of torpor-arousal. This study analyzes differential regulation of select histone modifications (e.g. phosphorylation, acetylation, methylation), and identifies post-translational modifications on purified histones using mass spectrometry from thirteen-lined ground squirrels (Ictidomys tridecemlineatus)...
February 28, 2017: Cryobiology
https://www.readbyqxmd.com/read/28237626/effects-of-cryopreservation-at-various-temperatures-on-the-survival-of-kelp-grouper-epinephelus-moara-embryos-from-fertilization-with-cryopreserved-sperm
#10
Yongsheng Tian, Zhangfan Chen, Jiang Tang, Huimin Duan, Jieming Zhai, Bo Li, Wenhui Ma, Jiangchun Liu, Yunxia Hou, Zhengxiang Sun
Fish embryo cryopreservation is highly important for the long-term preservation of genomic and genetic information; however, few successful cases of fish embryo cryopreservation have been reported over the past 60 years. This is the first study to use Epinephelus moara embryos from fertilization with cryopreserved sperm as experimental material. Embryos that developed to the 16-22 somite stage and tail-bud stage were treated with the vitrification solution PMG3T according to a five-step equilibration method and cryopreserved at various temperatures and storage duration...
February 22, 2017: Cryobiology
https://www.readbyqxmd.com/read/28223021/trehalose-improves-rabbit-sperm-quality-during-cryopreservation
#11
Zhendong Zhu, Xiaoteng Fan, Yang Pan, Yinghua Lu, Wenxian Zeng
High levels of reactive oxygen species are associated with spermatozoa cryopreservation, which bring damage to functional spermatozoa. The aim of the present study was to investigate whether and how the freezing extenders supplemented with trehalose was beneficial for the survival of rabbit spermatozoa. semen was diluted with Tris-citrate-glucose extender addition of different concentrations of trehalose. Addition of 100 mM trehaose significantly improved post-thaw rabbit sperm parameters, such as motility, acrosome integriy, membrane integrity and mitochondrial membrane potential...
February 20, 2017: Cryobiology
https://www.readbyqxmd.com/read/28216339/the-effect-of-tribulus-terrestris-extract-on-motility-and-viability-of-human-sperms-after-cryopreservation
#12
Atefeh Asadmobini, Mitra Bakhtiari, Sara Khaleghi, Farzaneh Esmaeili, Ali Mostafaei
BACKGROUND: Semen cryopreservation produces significant amounts of reactive oxygen species (ROS), which may lead to impairment of sperm morphology, function, and ultimately, male fertility. Since Tribulus terrestris has antioxidant and free-radical-scavenging properties, this study aims to reveal the effect of the Tribulus terrestris extract on motility and vitality of human sperms after cryopreservation. MATERIALS AND METHODS: Semen specimens from 80 healthy volunteers were divided into eight groups: fresh control (group I), freeze control (group II), groups III, IV, and V, which had 20, 40, and 50 μg/mL doses of Tribulus terrestris extract added before cryopreservation, and groups VI, VII, and VIII, which were supplemented by these extract doses after the freeze-thaw process...
February 17, 2017: Cryobiology
https://www.readbyqxmd.com/read/28209499/blastocyst-development-after-intracytoplasmic-sperm-injection-of-equine-oocytes-vitrified-at-the-germinal-vesicle-stage
#13
Heloísa Siqueira Canesin, Joao Gatto Brom-de-Luna, Young-Ho Choi, Isabel Ortiz, Mouhamadou Diaw, Katrin Hinrichs
We evaluated the meiotic and developmental competence of GV-stage equine oocytes vitrified under different conditions. In a preliminary study, using dimethyl sulfoxide (D), ethylene glycol (EG) and sucrose (S) as cryoprotectants, the maturation rate was higher for cumulus-oocyte complexes (COCs) held overnight before vitrification (37%) than for those vitrified immediately (14%; P < 0.05). Thereafter, all COCs were held overnight before vitrification. In Experiment 1 we compared 1 min (1 m) and 4 min (4 m) exposure to vitrification and warming solutions; oocytes that subsequently matured were fertilized by ICSI...
February 13, 2017: Cryobiology
https://www.readbyqxmd.com/read/28193448/cryopreservation-of-bufotes-viridis-embryos-by-vitrification
#14
Zahra Derakhshan, Mohsen Nokhbatolfoghahai, Shahla Zahiri
Loss of biodiversity among amphibians is a current concern. Our hypothesis is that the embryos of amphibian species at risk of extinction could be cryopreserved by vitrification, using methods which have proved successful with fish oocyte. To test this hypothesis, samples of four cryoprotectants - methanol (MeOH), dimethyl sulphoxide (Me2SO), propylene glycol (PG) and polyethylene glycol (PEG), some singly, some in combination, were plunged in liquid nitrogen for 5 min to find the best solution for vitrification...
February 11, 2017: Cryobiology
https://www.readbyqxmd.com/read/28192075/the-effect-of-additive-compounds-on-glycerol-induced-damage-to-human-chondrocytes
#15
Joshua Hahn, Leila Laouar, Janet A W Elliott, Gregory S Korbutt, Nadr M Jomha
High concentrations of cryoprotective agents are required for cryopreservation techniques such as vitrification. Glycerol is a common cryoprotective agent used in cryopreservation protocols but this agent is toxic at high concentrations. This work is an attempt to mitigate the toxic effects of high concentrations of glycerol on intact chondrocytes in human knee articular cartilage from total knee arthroplasty patients by simultaneous exposure to glycerol and a variety of additive compounds. The resulting cell viability in the cartilage samples as measured by membrane integrity staining showed that, in at least one concentration or in combination, all of the tested additive compounds (tetramethylpyrazine, ascorbic acid, chondroitin sulphate, glucosamine sulphate) were able to reduce the deleterious effects of glycerol exposure when examination of membrane integrity took place on a delayed time frame...
February 10, 2017: Cryobiology
https://www.readbyqxmd.com/read/28192076/thermo-mechanical-stress-analysis-of-cryopreservation-in-cryobags-and-the-potential-benefit-of-nanowarming
#16
Prem Solanki, John C Bischof, Yoed Rabin
Cryopreservation by vitrification is the only promising solution for long-term organ preservation which can save millions of lives across the world. One of the challenges in cryopreservation of large-size tissues and organs is to prevent fracture formation due to the tendency of the material to contract with temperature. The current study focuses on a pillow-like shape of a cryobag, while exploring various strategies to reduce thermo-mechanical stress during the rewarming phase of the cryopreservation protocol, where maximum stresses are typically found...
February 9, 2017: Cryobiology
https://www.readbyqxmd.com/read/28161239/cryopreservation-of-bovine-somatic-cells-using-antifreeze-polyamino-acid-carboxylated-poly-l-lysine
#17
Takuro Fujikawa, Takaaki Ando, Yuki Gen, Suong-Hyu Hyon, Chikara Kubota
Carboxylated poly-l-lysine (CPLL) is an ampholytic polymer compound, obtained by converting 65 mol% of amino groups to carboxyl groups after synthesizing ε-poly-l-lysine aqueous solution and succinic anhydride. CPLL has cryoprotective properties similar to those of anti-freeze protein. The addition of CPLL to freezing medium has been reported to improve the post-thawing survival rate of murine cells, human induced pluripotent stem (iPS) cells, embryonic stem (ES) cells and embryos. In this study, investigating CPLL for its effectiveness as a new cryoprotective material is aimed...
February 1, 2017: Cryobiology
https://www.readbyqxmd.com/read/28137430/0%C3%A2-%C3%A2-c-is-better-thawing-temperature-optimization-study-for-cancer-cryoablation-in-a-mouse-model-with-green-fluorescent-protein-labeled-lewis-lung-cancer
#18
Tian Zhou, Quanwang Li, Xiaoyi Chi, Linyi Li, Jianyu Rao, Kaiwen Hu
PURPOSE: There are two kinds of thawing temperatures commonly adopted in cancer cryosurgery. We attempted to compare their efficacy differences in this study to optimize the surgical method. METHOD: Forty-five C57BL/6 J mice with GFP-labeled Lewis lung cancer were randomized into three groups (n = 15 for each): control group, T0 group (thawing temperature 0 °C), and T40 group (thawing temperature 40 °C). Cryoablation was performed using a combined surgical system...
January 27, 2017: Cryobiology
https://www.readbyqxmd.com/read/28137429/full-term-potential-of-goat-in-vitro-produced-embryos-after-different-cryopreservation-methods
#19
José Carlos Ferreira-Silva, Marcelo Tigre Moura, Túlio Diego Silva, Luis Rennan Sampaio Oliveira, Adauto Chiamenti, Vicente José Figueirêdo Freitas, Marcos Antonio Lemos Oliveira
Cryopreservation of preimplantation embryos represents a major challenge due to their shape and relatively large cells. Embryo source and cryopreservation method are key factors to cryotolerance efficiency and few reports have investigated more promising protocols for goat embryos. The study was aimed to compare different cryopreservation methods for goat in vitro produced (IVP) embryos. Goat blastocysts were subjected to conventional freezing (CF), Dimethyl sulfoxide vitrification (DMSO-V) and Dimethylformamide vitrification (DMF-V)...
January 27, 2017: Cryobiology
https://www.readbyqxmd.com/read/28115174/effect-of-storage-conditions-on-the-ldl-effectiveness-in-ovine-sperm-cryopreservation
#20
P P N Snoeck, L C O Moura, M C Silva, M Machado-Neves, M I V Melo, L G D Heneine, M Henry
The present study evaluated the effect of storage conditions on the LDL efficacy for cryopreserving ovine sperm. In this way, we compared egg yolk extender with three different forms for LDL storing, LDL diluted in Tris-glucose extender and stored in frozen (i) and freeze-dried (ii) states and LDL stored pure and added into the extender prior to use (iii). We also tested the effect of two storage temperatures (-20 and -80 °C) and three storage times (30, 60, 120 d). Frozen and freeze-dried extenders containing LDL, as well as LDL stored pure, improved post-thaw sperm quality...
January 20, 2017: Cryobiology
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