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M V Santos, M Sansinena, J Chirife, N Zaritzky
The warming of cryopreserved samples supported by small volume devices is governed by heat transfer phenomena which are mathematically described by the solution of the transient heat conduction partial differential equations; the convective heat transfer coefficient (h) is an important parameter involved in the boundary condition which is related to the fluid dynamic behavior at the interface device-warming fluid (water, sucrose solution or air). Unfortunately, h values for small volume devices (i.e. Cryotop® ) have not been experimentally determined...
August 13, 2018: Cryobiology
Vidya Sagar Reddy, Brijesh Yadav, Chhote Lal Yadav, Mukul Anand, Dilip Kumar Swain, Devendra Kumar, Deepika Kritania, Arun Kumar Madan, Jitender Kumar, Sarvajeet Yadav
Cryopreservation results in substantial deterioration of heat shock protein 70 (HSP70) and ultra-structural changes in sperm organelles, resulting in a marked reduction in post-thaw semen quality. The present study was aimed to explicate the effect of sericin supplementation on expression profile of HSP70, redox status and post-thaw semen quality in Barbari goat. Five Barbari bucks were used to collect thirty semen ejaculates by using artificial vagina and each ejaculate was divided into three aliquots to which sericin was supplemented at 0% (Control), 0...
August 9, 2018: Cryobiology
Romualdo Sciorio, K J Thong, Susan J Pickering
This study investigates the utility of the Rapid-i closed device for vitrification of human blastocysts on day-5 (D5) and day-6 (D6) of development and the implantation and pregnancy rate following single blastocyst transfer (SBT) of warmed D5/D6 blastocysts. This retrospective cohort study was performed at Edinburgh Assisted Conception Programme, EFREC, Royal Infirmary of Edinburgh between January 2013 and January 2017. Good quality blastocysts were vitrified on D5 or D6 using Irvine Vitrification medium (Irvine Scientific-USA) and the Rapid-I closed Vitrification System™ (Vitrolife, Sweden)...
August 9, 2018: Cryobiology
Fa Ren, Tianyu Feng, Guichao Dai, Yanhua Wang, Haijing Zhu, Jianhong Hu
Cashmere goats, a unique biological resource in China, have the highest cashmere yield and best fiber quality in the world. The goal of this study was to determine the effects of cryopreserving with lycopene (LP) and alpha-lipoic acid (ALA) on the physiological characteristics of Cashmere goat spermatozoa. The results showed that sperm motility, acrosome integrity, membrane integrity, and mitochondrial activity of goat spermatozoa were greater in extenders containing 1.0 mg/mL LP and 10 μg/mL ALA (P < 0...
August 9, 2018: Cryobiology
Saritvich Panyaboriban, Theerawat Tharasanit, Vibuntita Chankitisakul, Theerawat Swangchan-Uthai, Mongkol Techakumphu
The cryopreservation of embryos is a technology developed for long-term genetic preservation. However, high sensitivity to low temperatures due to a large number of intracellular lipids within ruminant embryos compromises the success of this technique. The aim of this study was to examine the effects of using of lipolytic chemical agent forskolin, during in vitro producing of buffalo and bovine embryos on lipid contents, cryotolerance and subsequent developmental competence of these embryos. Buffalo and bovine oocytes were collected by the aspiration technique from follicles and submitted for in vitro fertilisation; the embryos were later divided into four experiments...
August 6, 2018: Cryobiology
Min-Rui Wang, Xin-Yi Hao, Lei Zhao, Zhen-Hua Cui, Gayle M Volk, Qiao-Chun Wang
Plant cryopreservation has provide secure back-ups of germplasm collections of vegetatively propagated crops. Often, recovery levels vary among laboratories when the same cryogenic procedures are used for the same genotypes. The present study investigated the effects of Apple stem grooving virus (ASGV) on shoot proliferation of in vitro stock cultures and recovery of cryopreserved shoot tips of 'Gala' apple. Results showed that virus infection reduced shoot proliferation of in vitro stock cultures and cell ability to regenerate normal shoots in cryopreserved shoot tips...
August 6, 2018: Cryobiology
Daniel Alexander Bizjak, Pia Jungen, Wilhelm Bloch, Marijke Grau
AIM: High glycerol cryopreservation of red blood cells (RBCs) reduces metabolic processes at ultralow temperatures but less is known regarding the effect of cryopreservation on RBC nitric oxide (NO) metabolism, haemorheological properties, structural behaviour and membrane fragility. METHODS: Blood from ten healthy participants was sampled, glycerolized and stored at -80 °C (SB). Aliquots were thawed and further processed after 4, 8 and 12 weeks, respectively...
August 3, 2018: Cryobiology
Konra Mueller, Matthew S Kokotilo, Jodi Carter, Aducio Thiesen, Karen L Madsen, Jacek Studzinski, Rachel G Khadaroo, Thomas A Churchill
We have developed a novel, intraluminal preservation solution that is tailored to the metabolic requirements of the intestine. This organ-specific solution addresses many of the problems associated with low temperature organ storage including energy, oxidative and osmotic stresses. However, conservation of energy levels remains one of the most difficult obstacles to overcome due to the inherent sensitivity of the mucosa to ischemia. Creatine-loading has become a popular and scientifically proven method of augmenting energy reserves in athletes performing anaerobic burst work activities...
August 1, 2018: Cryobiology
Charles Osei-Bempong, Ali E Ghareeb, Majlinda Lako, Francisco C Figueiredo, W John Armitage
Limbal stem cell (LSC) deficiency causes progressive loss of vision but may be treated by transplant of autologous LSCs. Cryopreservation has the potential to indefinitely extend the lifespan of LSCs allowing re-transplant in case of graft failure. In this study, we aimed to identify the optimal cryoprotectant and cryoprotectant concentration for LSC cultures. Suspension cultures derived from cadaveric corneoscleral rims were cooled to 4 °C with Me2 SO, propylene glycol or ethylene glycol at a concentration of 5%, 10% or 15%...
July 31, 2018: Cryobiology
Magda Teixeira, Loris Commin, Lucie Gavin-Plagne, Pierre Bruyère, Samuel Buff, Thierry Joly
Embryo cryopreservation media usually contain animal-derived products, such as bovine serum albumin (BSA). These products present two major disadvantages: an undefined variable composition and a risk of pathogen transmission. We aimed to evaluate the effect of replacing BSA in rabbit embryo rapid cooling "freezing" and warming media with a chemically defined medium with no animal-derived products: STEM ALPHA. Cryo3 ("Cryo3"). A total of 1540 rabbit morulae were divided into three cryopreservation groups (group 1: BSA, group 2: 20% Cryo3 and group 3: 100% Cryo3) and a fresh controls group...
July 23, 2018: Cryobiology
María Noel Meikle, Geraldine Schlapp, Alejo Menchaca, Martina Crispo
This study was conducted to compare the effect of minimum volume Spatula MVD vitrification (VIT) versus traditional slow freezing (SLF) of mouse embryos. A total of 2,617 8-cell in vivo derived and 2-cell in vitro produced B6D2 mouse embryos were subjected to freezing/thawing or vitrification/warming, while fresh embryos were used as control group. Embryo recovery, survival and development rate, pregnancy rate and offspring production were analyzed. In Experiment 1, 8-cell in vivo derived embryos were subjected to in vitro culture, resulting in greater survival and development rates at 3...
July 21, 2018: Cryobiology
Di Liu, Shenghui Zheng, Guantao Zheng, Qingyun Lv, Bin Shen, Xinpu Yuan, Yi-Hsuan Pan
Hibernation is an adaptive strategy used by some animals to cope with cold and food shortage. The heart rate, overall energy need, body temperature, and many other physiological functions are greatly reduced during torpor but promptly return to normal levels upon arousal. The heartbeat of torpid bats can be hundreds fold lower than that of active bats, indicating that hibernating bats have a remarkable ability to control excitation-contraction coupling in cardiac muscle. FKBP1B (calstabin 2), a peptidyl-prolyl cis-trans isomerase, is critical for the regulation of excitation-contraction coupling...
August 2018: Cryobiology
Ellen Cristina Rivas Leonel, Janice Miranda Vasconcellos Vilela, Daniela de Jesus Carrilho, Carolina Madeira Lucci
Ovarian tissue cryopreservation is a promising technique for fertility maintenance. The aim of this study was to compare the morphology of domestic cat ovarian follicles after tissue cryopreservation with ethylene glycol (EG) and dimethyl sulfoxide (Me2 SO). Ovaries from healthy adult cats undergoing elective ovariohysterectomy were used. Eight fragments were obtained from each pair of ovaries: two were used as fresh controls; three were submitted to fresh perfusion toxicity test and perfused with M199, 10% fetal calf serum and 0...
August 2018: Cryobiology
Chu W Kwang-Hua
We make some crucial remarks about the recent review by Buitink and Leprince (2004) [Cryobiology 48 (2004) 215-228] considering their false definition about the glass transition temperature based on the value of (shear) viscosity: 1014 Pa⋅s. The correct value is 1012 Pa⋅s as evidenced from or
August 2018: Cryobiology
Cheng-Fong Chen, Hui-Chun Chu, Chao-Ming Chen, Yu-Chi Cheng, Shang-Wen Tsai, Ming-Chau Chang, Wei-Ming Chen, Po-Kuei Wu
Freezing nitrogen ethanol composite (FNEC) showed effective cryoablative ability for bone tumor ex vivo and in vivo comparable to liquid nitrogen (LN). We therefore wished to compare the radiant cooling damage of the surrounding tissue between FNEC and LN. The evaluation of the radiant cooling damage was demonstrated human bone xenograft transplantation (HXT) in a mouse model. Characterizations and quantifications of the damaging effects on morphologic features and apoptosis of the cryoablative surrounding bone tissue, muscle and epidermal layer of skin were compared...
August 2018: Cryobiology
Vânia I B Castro, Rita Craveiro, Joana M Silva, Rui L Reis, Alexandre Paiva, Ana Rita C Duarte
Natural deep eutectic systems (NADES) are mostly composed of natural primary metabolites such as sugars, sugar alcohols, organic acids, amino acids and amines. These simple molecules have been identified in animals living in environments with extreme temperature amplitudes, being responsible for their survival at negative temperatures during winter. Herein, we report for the first time the use of NADES based on trehalose (Treh) and glycerol (Gly) in cryopreservation, as cryoprotective agents (CPA). The evaluation of the thermal behaviour of these eutectic systems, showed that NADES have a strong effect on the water crystallization/freezing and melting process, being able to reduce the number of ice crystals and hence ice crystal damage in cells, which is a crucial parameter for their survival, upon freezing...
August 2018: Cryobiology
Florence Guignot, Dominique Fortini, Stéphane Grateau, Cécile Burette, Virginie Dreyfus, Carole Moreau-Vauzelle, Pierrick Aupinel, Elisabeth Blesbois
Honeybees, major providers of pollination, are endangered in many areas. Embryo cryopreservation may be a very useful tool to maintain their genetic diversity. However, it is complex in insects, because embryos are chill sensitive and are surrounded by two protectant membranes, the chorion and vitelline. These membranes prevent penetration of cryoprotectant in the embryos. This study aimed to test different conditions of embryo preparation before cryopreservation, including low-frequency sonophoresis, a physical method of permeabilization, and passages through cryoprotectant solutions...
August 2018: Cryobiology
Yanfang Zhang, Gideon Ukpai, Alexandra Grigoropoulos, Matthew J Powell-Palm, Bradley P Weegman, Michael J Taylor, Boris Rubinsky
Ice-free vitreous cryopreservation (vitrification) is regarded as the principal method for avoiding ice crystallization damage in cryopreserved tissues and organs. We previously established the fundamental thermodynamics of isochoric (constant volume) systems for cryopreservation, and now extend this novel approach to vitrification in an isochoric system. This was achieved by measuring pressure changes in a 2 ml isochoric chamber containing a variety of aqueous solutions of the ubiquitous cryoprotective additives (CPA), dimethyl sulfoxide (Me2 SO) and Propane-diol...
August 2018: Cryobiology
Hao Xu, Wenyue Cao, Liang Huang, Min Xiao, Yang Cao, Lei Zhao, Na Wang, Jianfeng Zhou
Chimeric antigen receptor T (CART) cell therapy has emerged as a potentially curative "drug" for cancer treatment. Cryopreservation of CART cells is necessary for their clinical application. Systematic studies on the effects of cryopreservation on the antitumor function of CART cells are lacking. Therefore, we compared the phenotypes and functions of CART cells that were cryopreserved during ex vivo expansion with those of freshly isolated populations. T cells expressing an anti-B-cell-maturation-antigen (BCMA) chimeric antigen receptor (CAR) were expanded in vitro for 10 days and then cryopreserved...
August 2018: Cryobiology
Danielle C C Brito, Sheyla F S Domingues, Ana Paula R Rodrigues, José R Figueiredo, Regiane R Santos, Júlio C Pieczarka
We aimed to evaluate the effect of three extracellular cryoprotectants on the morphology of vitrified feline preantral follicles. Feline ovarian fragments (0.5 × 2 × 2 mm) collected from five domestic adult cats subjected to ovariohysterectomy for routine castration were vitrified with ethylene glycol (EG) 40% combined or not with sucrose (0.1 or 0.5 M), trehalose (0.1 or 0.5 M), or raffinose (0.1 M). After vitrification using the solid-surface method and warming of the tissues, cryoprotectants were washed out of the ovarian tissues, which were fixed for histological analysis...
August 2018: Cryobiology
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