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Cryobiology

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https://www.readbyqxmd.com/read/27908687/fertility-response-evaluation-of-artificial-insemination-methods-in-sheep-with-fresh-and-frozen-thawed-semen
#1
Reza Masoudi, Ahmad Zare Shahneh, Armin Towhidi, Hamid Kohram, Abbas Akbarisharif, Mohsen Sharafi
The aim of this study was to evaluate the fertility response of artificial insemination (AI) methods with fresh and frozen sperm in sheep. In experiment 1, one hundred and fifty fat tailed Zandi ewes were assigned into 3 equal groups and inseminated with three AI methods consisting of vaginal, laparoscopic and trans-cervical AI with fresh semen. In experiment 2, a factorial study (3 AI methods × 2 extenders) was used to analyze the effects of three AI methods and two freezing extenders containing soybean lecithin (SL) or Egg yolk (EY) on reproductive performance of 300 fat tailed Zandi ewes...
November 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27908686/effect-of-different-vitrification-solutions-and-cryodevices-on-viability-and-subsequent-development-of-buffalo-oocytes-vitrified-at-the-germinal-vesicle-gv-stage
#2
Amr S El-Shalofy, Adel R Moawad, Gamal M Darwish, Sayed T Ismail, Aly B A Badawy, Magdy R Badr
The cryopreservation of immature oocytes would generate a readily available, non-seasonal source of female gametes for research and reproduction. In domestic animals, the most promising results on oocyte cryopreservation have been reported in cattle, few studies have been conducted on buffalo. The aim of the present study was to compare the use of different vitrification solutions and various cryodevices on viability and developmental competence of buffalo oocytes vitrified at the germinal vesicle (GV) stage...
November 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27908685/royal-jelly-supplemented-soybean-lecithin-based-extenders-improve-post-thaw-quality-and-incubation-resilience-of-goat-spermatozoa
#3
Selim Alcay, M Berk Toker, N Tekin Onder, Elif Gokce
The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thaw quality and incubation resilience of goat spermatozoa. Semen samples were collected from five goats. Pooled semen were diluted with soybean lecithin-based extender without RJ (control) or supplemented with different concentrations (0.25, 0.5 and 0.75%) of RJ (RJ0.25, RJ0.5, RJ0.75 respectively), at a final concentration of 150 × 10(6) spermatozoon/mL. Semen samples were assessed for sperm motility, plasma membrane integrity using hypoosmotic swelling test (HOST) damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)...
November 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27908684/identification-and-profiling-of-mirnas-in-overwintering-lissorhoptrus-oryzophilus-via-next-generation-sequencing
#4
Yang Shuang, Zhang Juhong, Wang Shang, Zhang Xinxin, Liu Yan, Xi Jinghui
MicroRNAs (miRNAs) are important regulators of various biological processes in organisms. Insects subjected to abiotic stress can regulate gene expression post-transcriptionally through the use of microRNAs. However, the role of miRNAs in response to cold stress in Lissorhoptrus oryzophilus Kuschel remains unknown. The rice water weevil, L. oryzophilus, is an invasive insect that is able to survive cold winters. To characterize changes in miRNAs in response to overwintering in L. oryzophilus, a comprehensive comparative analysis of microRNAs was performed involving an overwintering and a normal adult...
November 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27894899/dietary-flax-seed-oil-and-or-vitamin-e-improve-sperm-parameters-of-cloned-goats-following-freezing-thawing
#5
Rohollah Kargar, Mohsen Forouzanfar, Gholamreza Ghalamkari, Mohammad Hossein Nasr Esfahani
Semen cryopreservation is affected by individual differences and use of clones animal from the same source is the main tool to eliminate genetic variation. Among many nutrients that are necessary for fertility, essential fatty acids and antioxidants are vital for production of healthy sperm by improving sperm membrane integrity and protecting sperm from oxidative stress. The goal of the current study was to investigate whether a flax seed oil or/and Vitamin E dietary supplementation could improve semen quality of cloned bucks following semen cryopreservation...
November 25, 2016: Cryobiology
https://www.readbyqxmd.com/read/27890704/cryopreservation-of-adult-cervid-testes
#6
Lavanya Pothana, Lalitha Devi, Sandeep Goel
Several species of cervids are currently classified as threatened or endangered due to a rapid decline in their populations. Sperm cryopreservation, in association with assisted reproductive technologies, can find application for the conservation of endangered cervids. In cases of unsuccessful sperm retrieval through other means prior to the death of the animal, adult testis is the only source of sperm. Recovery of viable sperm from adult testes depends on the effective preservation of testicular tissues through optimization of cryopreservation protocols...
November 25, 2016: Cryobiology
https://www.readbyqxmd.com/read/27871847/fatty-acids-composition-in-erythrocyte-membranes-of-athletes-after-one-and-after-a-series-of-whole-body-cryostimulation-sessions
#7
Magdalena Kepinska, Joanna Gdula-Argasinska, Zbigniew Dabrowski, Marta Szarek, Wanda Pilch, Agnieszka Kreska-Korus, Zbigniew Szygula
Whole body cryotherapy (WBC) is a treatment often used by athletes as part of biological renewal. Despite the large interest in this form therapy there is still a lack of information on the effects of WBC on the concentration of fatty acids in erythrocyte membranes. Our study aimed at comparing the fatty acids (FA) composition of erythrocyte membranes of athletes after one session and after a series of sessions of whole body cryostimulation. In our study small changes in the level of total cholesterol (decrease) were observed 24 h after a single session...
November 18, 2016: Cryobiology
https://www.readbyqxmd.com/read/27871846/the-oxidant-antioxidant-equilibrium-activities-of-selected-lysosomal-enzymes-and-activity-of-acute-phase-protein-in-peripheral-blood-of-18-year-old-football-players-after-aerobic-cycle-ergometer-test-combined-with-ice-water-immersion-or-recovery-at-room-temperature
#8
Paweł Sutkowy, Alina Woźniak, Tomasz Boraczyński, Michał Boraczyński, Celestyna Mila-Kierzenkowska
The goal of the study was to evaluate the effect of an aerobic exercise bout followed by ice-water immersion or recovery at room temperature on the redox state, activities of selected lysosomal enzymes and activity of α1-antitrypsin (AAT) in the blood of healthy sportsmen. Eleven amateur football players aged 18 were randomly assigned to two similar 30-min aerobic cycle ergometer tests followed by a recovery at room temperature (20 °C; Experiment 1) or ice-water immersion (3 °C, 5 min; Experiment 2). Peripheral blood was collected three times during both study experiments: before (baseline), as well as 20 and 40 min after the recovery or immersion...
November 18, 2016: Cryobiology
https://www.readbyqxmd.com/read/27847317/detecting-seasonal-variation-of-antifreeze-protein-distribution-in-rhagium-mordax-using-immunofluorescence-and-high-resolution-microscopy
#9
J L Buch, H Ramløv
Larvae of the blackspotted pliers support beetle, Rhagium mordax, were collected monthly, for the duration of 2012 and fixed. The larvae were embedded in paraffin wax and sectioned. Using fluorophore-coupled antibodies specific to the R. mordax antifreeze protein, RmAFP1, sections were visualised with UV reflected light microscopy. An automated software analysis method was developed in order to discard autofluorescence, and quantify fluorescence from bound antibodies. The results show that R. mordax cuticle and gut exhibit a higher degree of fluorophore-bound fluorescence during summer, than in the cold months...
November 12, 2016: Cryobiology
https://www.readbyqxmd.com/read/27840093/twig-pre-harvest-temperature-significantly-influences-effective-cryopreservation-of-vaccinium-dormant-buds
#10
Maria M Jenderek, Justin D Tanner, Barbara D Ambruzs, Mark West, Joseph D Postman, Kim E Hummer
Cryopreservation of temperate woody-plant material by dormant buds is less expensive than using shoot tips isolated from tissue cultured plants; however currently, dormant buds are used only for preservation of selected temperate tree and shrub species. Using dormant buds could be an efficient strategy for long-term preservation of blueberry (Vaccinium L.) genetic resources. In this study, viability of V. hybrid 'Northsky' (PI 554943) dormant buds was evaluated at 30 harvest dates over three consecutive fall/winter seasons to determine the optimal harvest time that promotes high post cryopreservation viability...
November 10, 2016: Cryobiology
https://www.readbyqxmd.com/read/27818266/optimization-of-the-cryopreservation-of-dromedary-camel-semen-cryoprotectants-and-their-concentration-and-equilibration-times
#11
Clara Malo, Elizabeth G Crichton, Julian A Skidmore
Research into an optimal cryoprotectant, its concentration and equilibration time underlies the successful cryopreservation of dromedary camel spermatozoa. This study assessed the cryo-efficiency of different cryoprotectants, their concentration and equilibration time and any interactions. In experiment 1, semen samples (n = 4 males; 2 ejaculates/male) were frozen using Green Buffer containing one of four cryoprotectants (3% glycerol, ethylene glycol, methyl formamide, dimethyl sulfoxide) and using 4 equilibration times (10 min, 0...
November 3, 2016: Cryobiology
https://www.readbyqxmd.com/read/27789380/sensitivity-of-thermophysiological-models-of-cryoablation-to-the-thermal-and-biophysical-properties-of-tissues
#12
Jun Y Chan, Ean H Ooi
Advancement in biomedical simulation and imaging modality have catalysed the development of in silico predictive models for cryoablation. However, one of the main challenges in ensuring the accuracy of the model prediction is the use of proper thermal and biophysical properties of the patient. These properties are difficult to measure clinically and thus, represent significant uncertainty that can affect the model prediction. Motivated by this, a sensitivity analysis is carried out to identify the model parameters that have the most significant impact on the lesion size during cryoablation...
October 24, 2016: Cryobiology
https://www.readbyqxmd.com/read/27746166/development-of-sperm-cryopreservation-protocol-of-endangered-spiny-eel-mastacembelus-armatus-lacepede-1800-for-ex-situ-conservation
#13
M Mahfujur Rahman, M Rahmat Ali, M Rafiqul Islam Sarder, M Fazlul Awal Mollah, Najmus Sakib Khan
The study was conducted to develop sperm cryopreservation protocol for the endangered spiny eel, Mastacembelus armatus as well as to use the cryopreserved sperm in breeding. Activation of sperm was evaluated by observing motility in different osmolalities of NaCl solution (48-319 mOsmol/kg) and found that sperm motility decreased with increasing osmolality of the NaCl solution and it was totally inhibited at 319 mOsmol/kg. The highest motility and swimming duration of sperm were observed as 14.5 ± 0.5 min and 4...
October 13, 2016: Cryobiology
https://www.readbyqxmd.com/read/27729220/sensitivity-of-photosynthetic-processes-to-freezing-temperature-in-extremophilic-lichens-evaluated-by-linear-cooling-and-chlorophyll-fluorescence
#14
Josef Hájek, Miloš Barták, Jana Hazdrová, Marie Forbelská
Extremophilic lichens and their photosynthesizing photobionts from the cold regions of Earth are adapted to perform photosynthesis at subzero temperatures. To evaluate interspecific differences in the critical temperature for primary photochemical processes of photosynthesis, we exposed lichen thalli of Usnea antarctica, Usnea aurantiaco-atra, and Umbilicaria cylindrica to linear cooling from +20 to -50 °C at a constant rate of 2 °C min(-1). Simultaneously, two chlorophyll fluorescence parameters (FV/FM - potential yield of photosynthetic processes in photosystem II, ΦPSII - effective quantum yield of PS II) evaluating a gradual subzero temperature-induced decline in photosynthetic processes were measured by a modulated fluorometer...
October 10, 2016: Cryobiology
https://www.readbyqxmd.com/read/27729221/the-use-of-antifreeze-protein-type-iii-for-vitrification-of-in%C3%A2-vitro-matured-bovine-oocytes
#15
Dowglish F Chaves, Iana S Campelo, Mirelly M A S Silva, Maajid H Bhat, Darcio I A Teixeira, Luciana M Melo, Joanna M G Souza-Fabjan, Pascal Mermillod, Vicente J F Freitas
The aim of this study was to evaluate the use of antifreeze protein type III (AFP III) into vitrification medium on meiotic spindle morphology of in vitro matured bovine oocytes as well as the fertilization and blastocyst rates. Mature cumulus-oocyte complexes (COC) were distributed in four groups: control (untreated), vitrified without supplementation (AFP0) or supplemented with 500 (AFP500) or 1000 ng/mL (AFP1000) into vitrification solutions. Samples from each group were used to analyze the organization of meiotic spindle by confocal microscopy and the remaining COC were submitted to in vitro fertilization and culture for eight days...
October 8, 2016: Cryobiology
https://www.readbyqxmd.com/read/27725165/improved-development-by-melatonin-treatment-after-vitrification-of-mouse-metaphase-ii-oocytes
#16
Yue Zhang, Wei Li, Yongshun Ma, Dian Wang, Xiaoxue Zhao, Changjun Zeng, Ming Zhang, Xianyin Zeng, Qinggang Meng, Guangbin Zhou
The study was aimed to investigate the effect of melatonin on the development potential of mouse MII oocytes after cryopreservation. Mouse MII oocytes were subjected first to vitrification/warming and 2 h of in vitro culture (phase 1), then to parthenogenetic activation (PA) followed by in vitro culture of parthenogenetic embryos (phase 2). Different concentrations of melatonin (0, 10(-9), 10(-6) mol/L) were added to the medium during either phase 1, phase 2 or both phases. The fresh oocytes were used as control...
October 8, 2016: Cryobiology
https://www.readbyqxmd.com/read/27717656/combined-effect-of-permeant-and-non-permeant-cryoprotectants-on-the-quality-of-frozen-thawed-chicken-sperm
#17
Fabio Mosca, Manuela Madeddu, Ahmad Abdel Sayed, Luisa Zaniboni, Nicolaia Iaffaldano, Silvia Cerolini
The aim of this study was to assess the combined effect of dimethylacetamide (DMA) and two non-permeating cryoprotective agents, trehalose and sucrose, on the quality of post-thaw chicken semen. Adult Lohmann roosters (n = 27) were used. Semen was processed according to the following treatments: Lake pre-freezing extender +6% DMA (LPF, control treatment), LPF + 0.1 M trehalose (LPF-T treatment), LPF + 0.1 M sucrose (LPF-S treatment) and LPF + 0.1 M trehalose + 0.1 M sucrose (LPF-TS treatment)...
October 4, 2016: Cryobiology
https://www.readbyqxmd.com/read/27693391/replacement-of-serum-with-ocular-fluid-for-cryopreservation-of-immature-testes
#18
Lavanya Pothana, Lalitha Devi, Naresh Kumar Venna, Niharika Pentakota, Vivek Phani Varma, Jedy Jose, Sandeep Goel
Cryopreservation of immature testis is a feasible approach for germplasm preservation of male animals. Combinations of dimethyl sulfoxide (DMSO) and foetal bovine serum (FBS) are used for testis cryopreservation. However, an alternative to FBS is needed, because FBS is expensive. Buffalo ocular fluid (BuOF), a slaughter house by-product, could be an economical option. The objective of the present study was to assess whether BuOF can replace FBS for cryopreservation of immature mouse (Mus musculus), rat (Rattus norvegicus), and buffalo (Bubalus bubalis) testes...
September 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27693236/bovine-epididymal-spermatozoa-resistance-to-cryopreservation-and-binding-ability-to-oviductal-cells
#19
A T M Cunha, J O Carvalho, N R Kussano, C F Martins, G B Mourão, M A N Dode
In this study we examined quality, longevity and ability of epididymal sperm (EP) to bind to oviduct explants (OE) after cooling and cryopreservation. Ejaculated (EJ) and EP sperm from seven bulls were evaluated before, during and after cryopreservation for total (TM), progressive motility (PM), sperm morphology, plasma membrane integrity (PMI) and acrosome integrity (ACI). For longevity, cryopreserved EP, EJ and a third group of cells in which EP spermatozoa were incubated with seminal plasm (SP) for 10 min after thawing (EPP group), were compared, and the groups were analyzed at 0, 3, 6, and 24 h for all parameters...
September 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27660063/the-effect-of-me2so-overexposure-during-cryopreservation-on-hos-te85-and-hmsc-viability-growth-and-quality
#20
Timothy J Morris, Andrew Picken, Duncan M C Sharp, Nigel K H Slater, Christopher J Hewitt, Karen Coopman
With the cell therapy industry continuing to grow, the ability to preserve clinical grade cells, including mesenchymal stem cells (MSCs), whilst retaining cell viability and function remains critical for the generation of off-the-shelf therapies. Cryopreservation of MSCs, using slow freezing, is an established process at lab scale. However, the cytotoxicity of cryoprotectants, like Me2SO, raises questions about the impact of prolonged cell exposure to cryoprotectant at temperatures >0 °C during processing of large cell batches for allogenic therapies prior to rapid cooling in a controlled rate freezer or in the clinic prior to administration...
September 20, 2016: Cryobiology
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