Read by QxMD icon Read

BMC Microbiology

Alberto Ramírez Mata, César Millán Pacheco, José F Cruz Pérez, Martha Minjárez Sáenz, Beatriz E Baca
BACKGROUND: The cyclic-di-GMP (c-di-GMP) second messenger exemplifies a signaling system that regulates many bacterial behaviors of key importance; among them, c-di-GMP controls the transition between motile and sessile life-styles in bacteria. Cellular c-di-GMP levels in bacteria are regulated by the opposite enzymatic activities of diguanylate cyclases and phosphodiesterases, which are proteins that have GGDEF and EAL domains, respectively. Azospirillum is a genus of plant-growth-promoting bacteria, and members of this genus have beneficial effects in many agronomically and ecologically essential plants...
March 9, 2018: BMC Microbiology
Lucy Cheng, Laura J Marinelli, Noël Grosset, Sorel T Fitz-Gibbon, Charles A Bowman, Brian Q Dang, Daniel A Russell, Deborah Jacobs-Sera, Baochen Shi, Matteo Pellegrini, Jeff F Miller, Michel Gautier, Graham F Hatfull, Robert L Modlin
BACKGROUND: A remarkable exception to the large genetic diversity often observed for bacteriophages infecting a specific bacterial host was found for the Cutibacterium acnes (formerly Propionibacterium acnes) phages, which are highly homogeneous. Phages infecting the related species, which is also a member of the Propionibacteriaceae family, Propionibacterium freudenreichii, a bacterium used in production of Swiss-type cheeses, have also been described and are common contaminants of the cheese manufacturing process...
March 1, 2018: BMC Microbiology
Qingfeng Li, Tao Peng, Gabriele Klug
BACKGROUND: A major role of the PhyR-NepR-σ(EcfG) cascade in the general stress response was demonstrated for some bacterial species and considered as conserved in Alphaproteobacteria. The σ(EcfG) factor activates its target genes in response to diverse stresses and NepR represents its anti-sigma factor. PhyR comprises a response regulator domain and a sigma factor domain and acts as anti-sigma factor antagonist. The facultative phototrophic alphaproteobacterium Rhodobacter sphaeroides harbours a PhyR homolog in the same genomic context as found in other members of this class...
February 27, 2018: BMC Microbiology
Oddvar Oppegaard, Haima Mylvaganam, Steinar Skrede, Bård Reiakvam Kittang
BACKGROUND: During the past decades, Streptococcus dysgalactiae subspecies equisimilis (SDSE) has been increasingly recognized as an important human pathogen. Osteoarticular infections is one of the predominant disease manifestations of SDSE, but the pathogenetic rationale for its arthritogenicity has yet to be unravelled. We aimed to explore if the rising incidence of osteoarticular infections caused by this pathogen in our region emanated from clonal expansion of strains with enhanced tropism for bone and joint tissue components or orthopaedic implants...
February 27, 2018: BMC Microbiology
Corrin V Wallis, Preena Lowden, Zoe V Marshall-Jones, Anthony C Hilton
BACKGROUND: Salmonella enterica is a recognised cause of diarrhoea in dogs and humans, yet the potential for transfer of salmonellosis between dogs and their owners is unclear, with reported evidence both for and against Salmonella as a zoonotic pathogen. A collection of 174 S. enterica isolates from clinical infections in humans and dogs were analysed for serotype distribution, carbon source utilisation, chemical and antimicrobial sensitivity profiles. The aim of the study was to understand the degree of conservation in phenotypic characteristics of isolates across host species...
February 26, 2018: BMC Microbiology
Wanyue Xu, Yaping Pan, Qiufang Xu, Yun Wu, Jiayu Pan, Jingya Hou, Li Lin, Xiaolin Tang, Chen Li, Jingbo Liu, Dongmei Zhang
BACKGROUND: Porphyromonas gingivalis (P. gingivalis), one of the main pathogenic bacteria involved in periodontitis, induces the expression of intercellular adhesion molecule - 1 (ICAM-1) and monocyte-endothelial cell adhesion. This effect plays a pivotal role in atherosclerosis development. Macrophage migration inhibitory factor (MIF) is a multifunctional cytokine and critically affects atherosclerosis pathogenesis. In this study, we tested the involvement of MIF in the P. gingivalis ATCC 33277-enhanced adhesive properties of endothelial cells...
February 26, 2018: BMC Microbiology
Yanbin Liu, Chong Mei John Koh, Sihui Amy Yap, Minge Du, Mya Myintzu Hlaing, Lianghui Ji
BACKGROUND: Rhodotorula toruloides is an outstanding producer of lipids and carotenoids. Currently, information on the key metabolic pathways and their molecular basis of regulation remains scarce, severely limiting efforts to engineer it as an industrial host. RESULTS: We have adapted Agrobacterium tumefaciens-mediated transformation (ATMT) as a gene-tagging tool for the identification of novel genes in R. toruloides. Multiple factors affecting transformation efficiency in several species in the Pucciniomycotina subphylum were optimized...
February 21, 2018: BMC Microbiology
Rachael Lappan, Kara Imbrogno, Chisha Sikazwe, Denise Anderson, Danny Mok, Harvey Coates, Shyan Vijayasekaran, Paul Bumbak, Christopher C Blyth, Sarra E Jamieson, Christopher S Peacock
BACKGROUND: Recurrent acute otitis media (rAOM, recurrent ear infection) is a common childhood disease caused by bacteria termed otopathogens, for which current treatments have limited effectiveness. Generic probiotic therapies have shown promise, but seem to lack specificity. We hypothesised that healthy children with no history of AOM carry protective commensal bacteria that could be translated into a specific probiotic therapy to break the cycle of re-infection. We characterised the nasopharyngeal microbiome of these children (controls) in comparison to children with rAOM (cases) to identify potentially protective bacteria...
February 20, 2018: BMC Microbiology
Bin He, Zhihong Hu, Long Ma, Haoran Li, Mingqiang Ai, Jizhong Han, Bin Zeng
BACKGROUND: The gene expression profile and metabolic pathways of Aspergillus oryzae underlying the anatomical and morphological differentiation across different growth stages have not been fully characterized. The rapid development of next-generation sequencing technologies provides advanced knowledge of the genomic organization of A. oryzae. RESULTS: In this study, we characterized the growth and development of A. oryzae at different growth stages, including the adaptive phase, logarithmic phase, and stationary phase...
February 14, 2018: BMC Microbiology
Gang Feng, Tian Xie, Xin Wang, Jiuyuan Bai, Lin Tang, Hai Zhao, Wei Wei, Maolin Wang, Yun Zhao
BACKGROUND: Soil contaminated with the heavy metal Cadmium (Cd) is a widespread problem in many parts of the world. Based on metagenomic analysis, we investigated the functional potential and structural diversity of the microbial community in Cd-contaminated and non-contaminated soil samples and we explored the associated metabolic pathway network in cluster of orthologous groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG). RESULTS: The results showed that microorganisms in these soils were quite abundant, and many of them possessed numerous physiological functions...
February 13, 2018: BMC Microbiology
Phattharaphon Wongphutorn, Chariya Chomvarin, Banchob Sripa, Wises Namwat, Kiatichai Faksri
BACKGROUND: Two-thirds of the world's population is thought to be infected by Helicobacter pylori. Although most people infected with H. pylori are asymptomatic, this pathogen is associated with several gastric pathologies including cancer. The risk factors for colonization are still unclear and the genetic diversity within individual hosts has never been clearly investigated. RESULT: This study determined the prevalence of, and explored risk factors for, H. pylori infection directly from paired saliva (n = 110) and stool (n = 110) samples from asymptomatic persons in Northeast Thailand...
January 30, 2018: BMC Microbiology
David B H Barton, Danae Georghiou, Neelam Dave, Majed Alghamdi, Thomas A Walsh, Edward J Louis, Steven S Foster
BACKGROUND: Microbial arrays, with a large number of different strains on a single plate printed with robotic precision, underpin an increasing number of genetic and genomic approaches. These include Synthetic Genetic Array analysis, high-throughput Quantitative Trait Loci (QTL) analysis and 2-hybrid techniques. Measuring the growth of individual colonies within these arrays is an essential part of many of these techniques but is useful for any work with arrays. Measurement is typically done using intermittent imagery fed into complex image analysis software, which is not especially accurate and is challenging to use effectively...
January 24, 2018: BMC Microbiology
Ramesh K Vuppada, Colby R Hansen, Kirsta A P Strickland, Keilen M Kelly, William R McCleary
BACKGROUND: Phosphate is an essential compound for life. Escherichia coli employs a signal transduction pathway that controls the expression of genes that are required for the high-affinity acquisition of phosphate and the utilization of alternate sources of phosphorous. These genes are only expressed when environmental phosphate is limiting. The seven genes for this signaling pathway encode the two-component regulatory proteins PhoB and PhoR, as well as the high-affinity phosphate transporter PstSCAB and an auxiliary protein called PhoU...
January 19, 2018: BMC Microbiology
Dong-Ri Piao, Xi Liu, Dong-Dong Di, Pei Xiao, Zhong-Zhi Zhao, Li-Qing Xu, Guo-Zhong Tian, Hong-Yan Zhao, Wei-Xing Fan, Bu-Yun Cui, Hai Jiang
BACKGROUND: Brucellosis incidence in China is divided into three stages: high incidence (1950s-1960s), decline (1970s-1980s), and re-emergence (1990s-2010s). At the re-emergence stage, Brucellosis incidence grew exponentially and spread to all 32 provinces. We describe the magnitude and the etiological distribution changes in mainland China by genotyping data and emphasize its recent reemergence. We also provide the genetic diversity and molecular epidemiological characteristics of Brucella...
January 10, 2018: BMC Microbiology
Chaitra Shankar, Balaji Veeraraghavan, Laura Eve Buchnan Nabarro, Raji Ravi, Naveen Kumar Devanga Ragupathi, Priscilla Rupali
BACKGROUND: Hypervirulent K. pneumoniae (hvKp) causes severe community acquired infections, predominantly in Asia. Though initially isolated from liver abscesses, they are now prevalent among invasive infections such as bacteraemia. There have been no studies reported till date on the prevalence and characterisation of hvKp in India. The objective of this study is to characterise the hypervirulent strains isolated from bacteraemic patients for determination of various virulence genes and resistance genes and also to investigate the difference between healthcare associated and community acquired hvKp with respect to clinical profile, antibiogram, clinical outcome and molecular epidemiology...
January 8, 2018: BMC Microbiology
Federica Carraturo, Olga De Castro, Jacopo Troisi, Adriana De Luca, Armando Masucci, Paola Cennamo, Marco Trifuoggi, Francesco Aliberti, Marco Guida
BACKGROUND: Drinking tea constitutes a tradition which is deeply rooted in the culture of several countries. Moreover, in recent years, tea consumption is growing all over the world. Improper herbal tea storage (long periods, humid environments) represents a relevant health hazard for consumers because of the growth of bacteria and molds. RESULTS: This study analyzed 32 samples of commercially available black and green teas - purchased from southern Italy markets and online-shops - and the monitoring of microbiological quality of the tea bag content was performed...
January 5, 2018: BMC Microbiology
Xiaobo Yu, Rebecca R Noll, Barbara P Romero Dueñas, Samual C Allgood, Kristi Barker, Jeffrey L Caplan, Matthias P Machner, Joshua LaBaer, Ji Qiu, M Ramona Neunuebel
BACKGROUND: The intracellular bacterial pathogen Legionella pneumophila proliferates in human alveolar macrophages, resulting in a severe pneumonia termed Legionnaires' disease. Throughout the course of infection, L. pneumophila remains enclosed in a specialized membrane compartment that evades fusion with lysosomes. The pathogen delivers over 300 effector proteins into the host cell, altering host pathways in a manner that sets the stage for efficient pathogen replication. The L. pneumophila effector protein AnkX targets host Rab GTPases and functions in preventing fusion of the Legionella-containing vacuole with lysosomes...
January 5, 2018: BMC Microbiology
Joo Shun Tan, Sahar Abbasiliasi, Saeid Kadkhodaei, Yew Joon Tam, Teck-Kim Tang, Yee-Ying Lee, Arbakariya B Ariff
BACKGROUND: Demand for high-throughput bioprocessing has dramatically increased especially in the biopharmaceutical industry because the technologies are of vital importance to process optimization and media development. This can be efficiently boosted by using microtiter plate (MTP) cultivation setup embedded into an automated liquid-handling system. The objective of this study was to establish an automated microscale method for upstream and downstream bioprocessing of α-IFN2b production by recombinant Escherichia coli...
January 4, 2018: BMC Microbiology
Stanley S Nyirenda, Bernard M Hang Ombe, Edgar Simulundu, Evans Mulenga, Ladslav Moonga, Robert S Machang U, Gerald Misinzo, Bukheti S Kilonzo
BACKGROUND: Plague is a flea-borne zoonotic and invasive disease caused by a gram negative coccobacillus bacterium called Yersinia pestis. Plague has caused three devastating pandemics globally namely: the Justinian, Black Death and Oriental plague. The disease in the Eastern Province of Zambia has been reported in Nyimba and Sinda Districts in the past 15 years. The aim of this study was to investigate the molecular epidemiology of plague in the two affected districts. Polymerase Chain Reaction (PCR), targeting Plasminogen activator gene (pla gene) of Y...
January 4, 2018: BMC Microbiology
Kalaiselvi Thangavel, Preethi Radha Krishnan, Srimeena Nagaiah, Senthil Kuppusamy, Senthil Chinnasamy, Jude Sudhagar Rajadorai, Gopal Nellaiappan Olaganathan, Balachandar Dananjeyan
BACKGROUND: Renewable energy for sustainable development is a subject of a worldwide debate since continuous utilization of non-renewable energy sources has a drastic impact on the environment and economy; a search for alternative energy resources is indispensable. Microalgae are promising and potential alternate energy resources for biodiesel production. Thus, our efforts were focused on surveying the natural diversity of microalgae for the production of biodiesel. The present study aimed at identification, isolation, and characterization of oleaginous microalgae from shola forests of Nilgiri Biosphere Reserve (NBR), the biodiversity hot spot of India, where the microalgal diversity has not yet been systematically investigated...
January 3, 2018: BMC Microbiology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"