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BMC Microbiology

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https://www.readbyqxmd.com/read/29739319/characterization-of-biofilm-forming-capacity-and-resistance-to-sanitizers-of-a-range-of-e-coli-o26-pathotypes-from-clinical-cases-and-cattle-in-australia
#1
Salma A Lajhar, Jeremy Brownlie, Robert Barlow
BACKGROUND: The formation of biofilms and subsequent encasement of bacterial cells in a complex matrix can enhance resistance to antimicrobials and sterilizing agents making these organisms difficult to eradicate and control. The aim of this study was to evaluate and compare the capacity of 40 E. coli O26 isolates of enterohemorrhagic E. coli (EHEC, n = 27), potential EHEC (pEHEC, n = 3), atypical enteropathogenic E. coli (aEPEC, n = 8) and non-toxigenic E. coli (NTEC, n = 2) from human and cattle sources to form biofilms on different surfaces, and determine whether extracellular matrix (ECM) components (cellulose, curli), motility, prophage insertion in mlrA and cell surface hydrophobicity could influence biofilm formation...
May 8, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29739310/genetic-diversity-and-characterization-of-arsenic-resistant-endophytic-bacteria-isolated-from-pteris-vittata-an-arsenic-hyperaccumulator
#2
Yunfu Gu, Yingyan Wang, Yihao Sun, Ke Zhao, Quanju Xiang, Xiumei Yu, Xiaoping Zhang, Qiang Chen
BACKGROUND: Alleviating arsenic (As) contamination is a high-priority environmental issue. Hyperaccumulator plants may harbor endophytic bacteria able to detoxify As. Therefore, we investigated the distribution, diversity, As (III) resistance levels, and resistance-related functional genes of arsenite-resistant bacterial endophytes in Pteris vittata L. growing in a lead-zinc mining area with different As contamination levels. RESULTS: A total of 116 arsenite-resistant bacteria were isolated from roots of P...
May 8, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29720089/susceptibility-patterns-and-the-role-of-extracellular-dna-in-staphylococcus-epidermidis-biofilm-resistance-to-physico-chemical-stress-exposure
#3
Charles Ochieng' Olwal, Paul Oyieng' Ang'ienda, David Miruka Onyango, Daniel Otieno Ochiel
BACKGROUND: Over 65% of human infections are ascribed to bacterial biofilms that are often highly resistant to antibiotics and host immunity. Staphylococcus epidermidis is the predominant cause of recurrent nosocomial and biofilm-related infections. However, the susceptibility patterns of S. epidermidis biofilms to physico-chemical stress induced by commonly recommended disinfectants [(heat, sodium chloride (NaCl), sodium hypochlorite (NaOCl) and hydrogen peroxide (H2 O2 )] in domestic and human healthcare settings remains largely unknown...
May 2, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29678140/using-nanosims-coupled-with-microfluidics-to-visualize-the-early-stages-of-coral-infection-by-vibrio-coralliilyticus
#4
E Gibbin, A Gavish, I Domart-Coulon, E Kramarsky-Winter, O Shapiro, A Meibom, A Vardi
BACKGROUND: Global warming has triggered an increase in the prevalence and severity of coral disease, yet little is known about coral/pathogen interactions in the early stages of infection. The point of entry of the pathogen and the route that they take once inside the polyp is currently unknown, as is the coral's capacity to respond to infection. To address these questions, we developed a novel method that combines stable isotope labelling and microfluidics with transmission electron microscopy (TEM) and nanoscale secondary ion mass spectrometry (NanoSIMS), to monitor the infection process between Pocillopora damicornis and Vibrio coralliilyticus under elevated temperature...
April 20, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29673319/carbapenemase-producing-enterobacteriaceae-in-mexico-report-of-seven-non-clonal-cases-in-a-pediatric-hospital
#5
Alejandra Aquino-Andrade, Jocelin Merida-Vieyra, Eduardo Arias de la Garza, Patricia Arzate-Barbosa, Agustín De Colsa Ranero
BACKGROUND: Carbapenemases-producing Enterobacteriaceae (CPE) are a worldwide public health emergency. In Mexico, reports of CPE are limited, particularly in the pediatric population. Here, we describe the clinical, epidemiological, and molecular characteristics of seven consecutive cases in a third-level pediatric hospital in Mexico City over a four-month period during 2016. RESULTS: The Enterobacteriaceae identified were three Escherichia coli strains (producing OXA-232, NDM-1 and KPC-2), two Klebsiella pneumoniae strains (producing KPC-2 and NDM-1), one Klebsiella oxytoca strain producing OXA-48 and one Enterobacter cloacae strain producing NDM-1...
April 19, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29669519/molecular-characterization-and-new-genotypes-of-enterocytozoon-bieneusi-in-pet-chipmunks-eutamias-asiaticus-in-sichuan-province-china
#6
Lei Deng, Wei Li, Zhijun Zhong, Yijun Chai, Leli Yang, Hang Zheng, Wuyou Wang, Hualin Fu, Min He, Xiangming Huang, Zhicai Zuo, Ya Wang, Suizhong Cao, Haifeng Liu, Xiaoping Ma, Kongju Wu, Guangneng Peng
BACKGROUND: Enterocytozoon bieneusi, the most commonly identified microsporidian species in humans, is also identified in livestock, birds, rodents, reptiles, companion animals, even wastewater. However, there is no information available on occurrence of E. bieneusi in pet chipmunks. The aim of the present study was to determine the genotypes, molecular characterization of E. bieneusi in pet chipmunks, and assess the zoonotic potential. RESULTS: A total of 279 fecal specimens were collected from chipmunks from seven pet shops and one breeding facility in Sichuan province, China...
April 18, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29669516/a-recurrent-silent-mutation-implicates-feca-in-ethanol-tolerance-by-escherichia-coli
#7
Katherine M Lupino, Kymberleigh A Romano, Matthew J Simons, John T Gregg, Leanna Panepinto, Ghislaine M Cruz, Lauren Grajek, Gregory A Caputo, Mark J Hickman, Gregory B Hecht
BACKGROUND: An issue associated with efficient bioethanol production is the fact that the desired product is toxic to the biocatalyst. Among other effects, ethanol has previously been found to influence the membrane of E. coli in a dose-dependent manner and induce changes in the lipid composition of the plasma membrane. We describe here the characterization of a collection of ethanol-tolerant strains derived from the ethanologenic Escherichia coli strain FBR5. RESULTS: Membrane permeability assays indicate that many of the strains in the collection have alterations in membrane permeability and/or responsiveness of the membrane to environmental changes such as temperature shifts or ethanol exposure...
April 18, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29661152/genetic-variability-in-the-sdrd-gene-in-staphylococcus-aureus-from-healthy-nasal-carriers
#8
Clement Ajayi, Espen Åberg, Fatemeh Askarian, Johanna U E Sollid, Mona Johannessen, Anne-Merethe Hanssen
BACKGROUND: Staphylococcus aureus cell wall anchored Serine Aspartate repeat containing protein D (SdrD) is a member of the microbial surface component recognising adhesive matrix molecules (MSCRAMMs). It is involved in the bacterial adhesion and virulence. However the extent of genetic variation in S. aureus sdrD gene within isolates from healthy carriers are not known. The aim of this study was to evaluate allelic variation of the sdrD gene among S. aureus from healthy nasal carriers...
April 16, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29661149/development-of-the-tonsillar-microbiome-in-pigs-from-newborn-through-weaning
#9
Luis Carlos Pena Cortes, Rhiannon M LeVeque, Julie Funk, Terence L Marsh, Martha H Mulks
BACKGROUND: Porcine tonsils are lympho-epithelial tissues, colonized by numerous bacteria and viruses, that act as a reservoir for both host-specific pathogens and zoonotic pathogens with a high potential of transmission to humans. There are no existing studies describing the development of the tonsillar microbiome. We sequenced 16S rRNA genes from tonsillar samples of pigs to follow the development of the microbial communities from birth through weaning. Samples derived from sows were also analyzed to determine potential sources for the tonsil microbiome in piglets...
April 16, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29661138/a-coxiella-burnetii-phospholipase-a-homolog-plda-is-required-for-optimal-growth-in-macrophages-and-developmental-form-lipid-remodeling
#10
Christopher M Stead, Diane C Cockrell, Paul A Beare, Heather E Miller, Robert A Heinzen
BACKGROUND: Many gram-negative bacteria produce an outer membrane phospholipase A (PldA) that plays an important role in outer membrane function and is associated with virulence. RESULTS: In the current study, we characterized a pldA mutant of Coxiella burnetii, an intracellular gram-negative pathogen and the agent of human Q fever. The C. burnetti pldA open reading frame directs synthesis of a protein with conserved PldA active site residues. A C. burnetii ΔpldA deletion mutant had a significant growth defect in THP-1 macrophages, but not axenic medium, that was rescued by complementation...
April 16, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29653505/non-tuberculous-mycobacterium-species-causing-mycobacteriosis-in-farmed-aquatic-animals-of-south-africa
#11
Nomakorinte Gcebe, Anita L Michel, Tiny Motlatso Hlokwe
BACKGROUND: Mycobacteriosis caused by non-tuberculous mycobacteria (NTM), is among the most chronic diseases of aquatic animals. In addition, fish mycobacteriosis has substantial economic consequences especially in the aquaculture and fisheries industry as infections may significantly decrease production and trade. Some fish NTM pathogens are highly virulent and zoonotic; as such, infection of aquaria with these pathogens is a public health concern. In this study, we report isolation of nine different NTM species from sixteen aquatic animals including different fish species, frogs and a crocodile...
April 13, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29649975/characterising-resuscitation-promoting-factor-fluorescent-fusions-in-mycobacteria
#12
Iria Uhía, Nitya Krishnan, Brian D Robertson
BACKGROUND: Resuscitation promoting factor proteins (Rpfs) are peptidoglycan glycosidases capable of resuscitating dormant mycobacteria, and have been found to play a role in the pathogenesis of tuberculosis. However, the specific roles and localisation of each of the 5 Rpfs in Mycobacterium tuberculosis remain mostly unknown. In this work our aim was to construct fluorescent fusions of M. tuberculosis Rpf proteins as tools to investigate their function. RESULTS: We found that Rpf-fusions to the fluorescent protein mCherry are functional and able to promote cell growth under different conditions...
April 12, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29649970/correction-to-genetic-characterization-of-bacillus-anthracis-in-guizhou-province-southwest-of-china
#13
Shijun Li, Qing Ma, Hong Chen, Dingming Wang, Ying Liu, Xiaoyu Wei, Lv You, Guanghai Yao, Kecheng Tian, Guangpeng Tang
Upon publication of the original article (1) it was highlighted by the authors that a grant awarded to support the research work of the study was missed in the acknowledgements. It should also be acknowledged that the grant titled "Genotyping and Molecular Epidemiological Characteristic of Bacillus anthracis in Guizhou Province" awarded by the Program of Natural Science Foundation of Guizhou Province (Grant No. Qian Ke He J Word [2015] 2084)also contributed to the resources for this research. This has since been formally noted in this correction article...
April 12, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29625553/a-nutrient-dependent-division-antagonist-is-regulated-post-translationally-by-the-clp-proteases-in-bacillus-subtilis
#14
Norbert S Hill, Jason D Zuke, P J Buske, An-Chun Chien, Petra Anne Levin
BACKGROUND: Changes in nutrient availability have dramatic and well-defined impacts on both transcription and translation in bacterial cells. At the same time, the role of post-translational control in adaptation to nutrient-poor environments is poorly understood. Previous studies demonstrate the ability of the glucosyltransferase UgtP to influence cell size in response to nutrient availability. Under nutrient-rich medium, interactions with its substrate UDP-glucose promote interactions between UgtP and the tubulin-like cell division protein FtsZ in Bacillus subtilis, inhibiting maturation of the cytokinetic ring and increasing cell size...
April 6, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29621980/modulations-in-the-offspring-gut-microbiome-are-refractory-to-postnatal-synbiotic-supplementation-among-juvenile-primates
#15
Ryan M Pace, Amanda L Prince, Jun Ma, Benjamin D W Belfort, Alexia S Harvey, Min Hu, Karalee Baquero, Peter Blundell, Diana Takahashi, Tyler Dean, Paul Kievit, Elinor L Sullivan, Jacob E Friedman, Kevin Grove, Kjersti M Aagaard
BACKGROUND: We and others have previously shown that alterations in the mammalian gut microbiome are associated with diet, notably early life exposure to a maternal high fat diet (HFD). Here, we aimed to further these studies by examining alterations in the gut microbiome of juvenile Japanese macaques (Macaca fuscata) that were exposed to a maternal HFD, weaned onto a control diet, and later supplemented with a synbiotic comprised of psyllium seed and Enterococcus and Lactobacillus species...
April 5, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29614952/mutations-of-dnaa-boxes-in-the-orir-region-increase-replication-frequency-of-the-minir1-1-plasmid
#16
Yuan Yao, Sukhbold Enkhtsetseg, Ingvild Odsbu, Lifei Fan, Morigen Morigen
BACKGROUND: The MiniR1-1 plasmid is a derivative of the R1 plasmid, a low copy cloning vector. RESULTS: Nucleotide sequencing analysis shows that the MiniR1-1 plasmid is a 6316 bp circular double-stranded DNA molecule with an oriR1 (origin for replication). The plasmid carries the repA, tap, copA and bla genes, and genes for ORF1 and ORF2. MiniR1-1 contains eight DnaA-binding sites (DnaA-boxes). DnaA-box1 is in the oriR1 region and fully matched to the DnaA-box consensus sequence, and DnaA-box8, with one mismatch, is close to the copA gene...
April 3, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29609544/detection-of-ctx-m-15-harboring-escherichia-coli-isolated-from-wild-birds-in-tunisia
#17
Houssem Ben Yahia, Rym Ben Sallem, Ghassan Tayh, Naouel Klibi, Insaf Ben Amor, Haythem Gharsa, Abdellatif Boudabbous, Karim Ben Slama
BACKGROUND: The spreading of antibiotic resistant bacteria is becoming nowadays an alarming threat to human and animal health. There is increasing evidence showing that wild birds could significantly contribute to the transmission and spreading of drug-resistant bacteria. However, data for antimicrobial resistance in wild birds remain scarce, especially throughout Africa. The aims of this investigation were to analyze the prevalence of ESBL-producing E. coli in faecal samples of wild birds in Tunisia and to characterize the recovered isolates...
April 2, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29609542/gastrointestinal-microbial-community-changes-in-atlantic-cod-gadus-morhua-exposed-to-crude-oil
#18
Andrea Bagi, Even Sannes Riiser, Hilde Steine Molland, Bastiaan Star, Thomas H A Haverkamp, Magne Olav Sydnes, Daniela Maria Pampanin
BACKGROUND: The expansion of offshore oil exploration increases the risk of marine species being exposed to oil pollution in currently pristine areas. The adverse effects of oil exposure through toxic properties of polycyclic aromatic hydrocarbons (PAHs) have been well studied in Atlantic cod (Gadus morhua). Nevertheless, the fate of conjugated metabolites in the intestinal tract and their effect on the diversity of intestinal microbial community in fish is less understood. Here, we investigated the intestinal microbial community composition of Atlantic cod after 28 days of exposure to crude oil (concentration range 0...
April 2, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29580217/foreign-peptide-triggers-boost-in-pneumococcal-metabolism-and-growth
#19
Fauzy Nasher, Sunniva Förster, Efe C Yildirim, Denis Grandgirard, Stephen L Leib, Manfred Heller, Lucy J Hathaway
BACKGROUND: Nonencapsulated Streptococcus pneumoniae bacteria are successful colonizers of the human nasopharynx and often possess genes aliB-like ORF 1 and 2 in place of capsule genes. AliB-like ORF 2 binds peptide FPPQSV, found in Prevotella species, resulting in enhanced colonization. How this response is mediated is so far unknown. RESULTS: Here we show that the peptide increases expression of genes involved in release of host carbohydrates, carbohydrate uptake and carbohydrate metabolism...
March 27, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29580208/screening-for-inhibitors-of-mutacin-synthesis-in-streptococcus-mutans-using-fluorescent-reporter-strains
#20
Priyanka Premnath, Michael Reck, Kathrin Wittstein, Marc Stadler, Irene Wagner-Döbler
BACKGROUND: Within the polymicrobial dental plaque biofilm, bacteria kill competitors by excreting mixtures of bacteriocins, resulting in improved fitness and survival. Inhibiting their bacteriocin synthesis might therefore be a useful strategy to eliminate specific pathogens. We used Streptococcus mutans, a highly acidogenic inhabitant of dental plaque, as a model and searched for natural products that reduced mutacin synthesis. To this end we fused the promoter of mutacin VI to the GFP+ gene and integrated the construct into the genome of S...
March 27, 2018: BMC Microbiology
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