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BMC Microbiology

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https://www.readbyqxmd.com/read/28212623/lysozyme-enhances-the-bactericidal-effect-of-bp100-peptide-against-erwinia-amylovora-the-causal-agent-of-fire-blight-of-rosaceous-plants
#1
Jordi Cabrefiga, Emilio Montesinos
BACKGROUND: Fire blight is an important disease affecting rosaceous plants. The causal agent is the bacteria Erwinia amylovora which is poorly controlled with the use of conventional bactericides and biopesticides. Antimicrobial peptides (AMPs) have been proposed as a new compounds suitable for plant disease control. BP100, a synthetic linear undecapeptide (KKLFKKILKYL-NH2), has been reported to be effective against E. amylovora infections. Moreover, BP100 showed bacteriolytic activity, moderate susceptibility to protease degradation and low toxicity...
February 17, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28212615/molecular-pathways-underlying-inhibitory-effect-of-antimicrobial-peptide-nal-p-113-on-bacteria-biofilms-formation-of-porphyromonas-gingivalis-w83-by-dna-microarray
#2
Hong-Yan Wang, Li Lin, Li-Si Tan, Hui-Yuan Yu, Jya-Wei Cheng, Ya-Ping Pan
BACKGROUND: Wound-related infection remains a major challenge for health professionals. One disadvantage in conventional antibiotics is their inability to penetrate biofilms, the main protective strategy for bacteria to evade irradiation. Previously, we have shown that synthetic antimicrobial peptides could inhibit bacterial biofilms formation. RESULTS: In this study, we first delineated how Nal-P-113, a novel antimicrobial peptide, exerted its inhibitory effects on Porphyromonas gingivalis W83 biofilms formation at a low concentration...
February 17, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28212613/porphyromonas-gingivalis-can-invade-periodontal-ligament-stem-cells
#3
Chunling Pan, Junchao Liu, Hongyan Wang, Jia Song, Lisi Tan, Haijiao Zhao
BACKGROUND: Porphyromonas gingivalis is strongly associated with the development, progression, severity and recurrence of periodontitis. Periodontal ligament stem cells (PDLSCs) play an important role in the maintenance of periodontal tissue self-renewal and repair. The purpose of this study was to investigate the ability of P. gingivalis to infect PDLSCs using an in vitro monolayer model. METHODS: We separated and cultured primary PDLSCs using the tissue block with limiting dilution method...
February 17, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28209130/determination-of-antibacterial-activity-and-minimum-inhibitory-concentration-of-larval-extract-of-fly-via-resazurin-based-turbidometric-assay
#4
Chien Huey Teh, Wasi Ahmad Nazni, Ab Hamid Nurulhusna, Ahmad Norazah, Han Lim Lee
BACKGROUND: Antimicrobial resistance is currently a major global issue. As the rate of emergence of antimicrobial resistance has superseded the rate of discovery and introduction of new effective drugs, the medical arsenal now is experiencing shortage of effective drugs to combat diseases, particularly against diseases caused by the dreadful multidrug-resistant strains, such as the methicillin-resistant Staphylococcus aureus (MRSA). The ability of fly larvae to thrive in septic habitats has prompted us to determine the antibacterial activity and minimum inhibitory concentrations (MICs) of larval extract of flies, namely Lucilia cuprina, Sarcophaga peregrina and Musca domestica against 4 pathogenic bacteria [Staphylococcus aureus, methicillin-resistant S...
February 16, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28202049/costs-and-benefits-of-natural-transformation-in-acinetobacter-baylyi
#5
Nils Hülter, Vidar Sørum, Kristina Borch-Pedersen, Mikkel M Liljegren, Ane L G Utnes, Raul Primicerio, Klaus Harms, Pål J Johnsen
BACKGROUND: Natural transformation enables acquisition of adaptive traits and drives genome evolution in prokaryotes. Yet, the selective forces responsible for the evolution and maintenance of natural transformation remain elusive since taken-up DNA has also been hypothesized to provide benefits such as nutrients or templates for DNA repair to individual cells. RESULTS: We investigated the immediate effects of DNA uptake and recombination on the naturally competent bacterium Acinetobacter baylyi in both benign and genotoxic conditions...
February 15, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28202007/diffusible-substances-from-lactic-acid-bacterial-cultures-exert-strong-inhibitory-effects-on-listeria-monocytogenes-and-salmonella-enterica-serovar-enteritidis-in-a-co-culture-model
#6
Solomon H Mariam, Nigus Zegeye, Abraham Aseffa, Rawleigh Howe
BACKGROUND: Food-borne infections cause huge economic and human life losses. Listeria monocytogenes and Salmonella enterica serovar Enteritidis are among the top ranking pathogens causing such losses. Control of such infections is hampered by persistent contamination of foods and food-processing environments, resistance of pathogens to sanitizing agents, existence of heterogeneous populations of pathogens (including culturable and viable but non-culturable cells) within the same food items, and inability to detect all such pathogens by culture-based methods...
February 15, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28193172/comparison-of-pathogenic-and-non-pathogenic-enterococcus-cecorum-strains-from-different-animal-species
#7
Arne Jung, Martin Metzner, Martin Ryll
BACKGROUND: Enterococcus cecorum (EC) infection currently is one of the most important bacterial diseases of modern broiler chickens but can also affect ducks or other avian species. However, little is known concerning pathogenesis of EC and most studies concentrate on examinations of EC strains from broilers only. The objective of this study was to compare pathogenic and commensal EC strains from different animal species concerning different phenotypic and genotypic traits. RESULTS: Pathogenic and commensal EC strains were not clearly separated from each other in a phylogenetic tree based on partial sequences of the 16S-rRNA-gene and also based on the fatty acid profile determined with gas chromatography...
February 13, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28187702/adaptation-response-of-pseudomonas-fragi-on-refrigerated-solid-matrix-to-a-moderate-electric-field
#8
Wenbo Chen, Honghai Hu, Chunjiang Zhang, Feng Huang, Dequan Zhang, Hong Zhang
BACKGROUND: Moderate electric field (MEF) technology is a promising food preservation strategy since it relies on physical properties-rather than chemical additives-to preserve solid cellular foods during storage. However, the effectiveness of long-term MEF exposure on the psychrotrophic microorganisms responsible for the food spoilage at cool temperatures remains unclear. RESULTS: The spoilage-associated psychrotroph Pseudomonas fragi MC16 was obtained from pork samples stored at 7 °C...
February 10, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28173753/conjugative-type-ivb-pilus-recognizes-lipopolysaccharide-of-recipient-cells-to-initiate-papi-1-pathogenicity-island-transfer-in-pseudomonas-aeruginosa
#9
Toan Phuoc Hong, Michelle Q Carter, Paolo Struffi, Stefano Casonato, Youai Hao, Joseph S Lam, Stephen Lory, Olivier Jousson
BACKGROUND: Pseudomonas aeruginosa pathogenicity island 1 (PAPI-1) is one of the largest genomic islands of this important opportunistic human pathogen. Previous studies have shown that PAPI-1 encodes several putative virulence factors, including a major regulator of biofilm formation and antibiotic-resistance traits. PAPI-1 is horizontally transferable into recipient strains lacking this island via conjugation mediated by the specialized type IV pilus. The PAPI-1 encodes a cluster of ten genes associated with the synthesis and assembly of the type IV pilus...
February 7, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28158975/do-biofilm-communities-respond-to-the-chemical-signatures-of-fracking-a-test-involving-streams-in-north-central-arkansas
#10
Wilson H Johnson, Marlis R Douglas, Jeffrey A Lewis, Tara N Stuecker, Franck G Carbonero, Bradley J Austin, Michelle A Evans-White, Sally A Entrekin, Michael E Douglas
BACKGROUND: Unconventional natural gas (UNG) extraction (fracking) is ongoing in 29 North American shale basins (20 states), with ~6000 wells found within the Fayetteville shale (north-central Arkansas). If the chemical signature of fracking is detectable in streams, it can be employed to bookmark potential impacts. We evaluated benthic biofilm community composition as a proxy for stream chemistry so as to segregate anthropogenic signatures in eight Arkansas River catchments. In doing so, we tested the hypothesis that fracking characteristics in study streams are statistically distinguishable from those produced by agriculture or urbanization...
February 3, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28158967/high-virulence-sub-populations-in-pseudomonas-aeruginosa-long-term-cystic-fibrosis-airway-infections
#11
Siobhán O'Brien, David Williams, Joanne L Fothergill, Steve Paterson, Craig Winstanley, Michael A Brockhurst
BACKGROUND: Pseudomonas aeruginosa typically displays loss of virulence-associated secretions over the course of chronic cystic fibrosis infections. This has led to the suggestion that virulence is a costly attribute in chronic infections. However, previous reports suggest that overproducing (OP) virulent pathotypes can coexist with non-producing mutants in the CF lung for many years. The consequences of such within-patient phenotypic diversity for the success of this pathogen are not fully understood...
February 3, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28152976/origins-and-global-context-of-brucella-abortus-in-italy
#12
Giuliano Garofolo, Elisabetta Di Giannatale, Ilenia Platone, Katiuscia Zilli, Lorena Sacchini, Anna Abass, Massimo Ancora, Cesare Cammà, Guido Di Donato, Fabrizio De Massis, Paolo Calistri, Kevin P Drees, Jeffrey T Foster
BACKGROUND: Brucellosis is a common and chronic disease of cattle and other bovids that often causes reproductive disorders. Natural infection in cattle is caused by Brucella abortus and transmission typically occurs during abortions, calving, or nursing. Brucellosis is also a major zoonotic disease due to contamination of dairy products or contact with the tissues of infected animals. Brucellosis has been eradicated from most of the developed world in the last 40 years but persists in many regions-the disease remains prevalent in portions of Africa, the Middle East, Asia, and Central and South America, as well as in the Mediterranean basin...
February 2, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28148244/detailed-analysis-of-c-di-gmp-mediated-regulation-of-csgd-expression-in-salmonella-typhimurium
#13
Irfan Ahmad, Annika Cimdins, Timo Beske, Ute Römling
BACKGROUND: The secondary messenger cyclic di-GMP promotes biofilm formation by up regulating the expression of csgD, encoding the major regulator of rdar biofilm formation in Salmonella typhimurium. The GGDEF/EAL domain proteins regulate the c-di-GMP turnover. There are twenty- two GGDEF/EAL domain proteins in the genome of S. typhimurium. In this study, we dissect the role of individual GGDEF/EAL proteins for csgD expression and rdar biofilm development. RESULTS: Among twelve GGDEF domains, two proteins upregulate and among fifteen EAL domains, four proteins down regulate csgD expression...
February 2, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28143403/decision-criteria-for-maldi-tof-ms-based-identification-of-filamentous-fungi-using-commercial-and-in-house-reference-databases
#14
Anne-Cécile Normand, Carole Cassagne, Magali Gautier, Pierre Becker, Stéphane Ranque, Marijke Hendrickx, Renaud Piarroux
BACKGROUND: Several Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry protocols, which differ in identification criteria, have been developed for mold and dermatophyte identification. Currently, the most widely used approach is Bruker technology, although no consensus concerning the log(score) threshold has been established. Furthermore, it remains unknown how far increasing the number of spots to compare results might improve identification performance. In this study, we used in-house and Bruker reference databases as well as a panel of 422 isolates belonging to 126 species to test various thresholds...
January 31, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28143392/binding-site-of-restriction-modification-system-controller-protein-in-mollicutes
#15
Gleb Y Fisunov, Daria V Evsyutina, Valentin A Manuvera, Vadim M Govorun
BACKGROUND: Bacteria of the class Mollicutes underwent extreme reduction of genomes and gene expression control systems. Only a few regulators are known to date. In this work, we describe a novel group of transcriptional regulators that are distributed within different Mollicutes and control the expression of restriction-modification systems (RM-systems). RESULTS: We performed cross-species search of putative regulators of RM-systems (C-proteins) and respective binding sites in Mollicutes...
January 31, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28122502/occurrence-of-escherichia-coli-o157-h7-in-cattle-feces-and-contamination-of-carcass-and-various-contact-surfaces-in-abattoir-and-butcher-shops-of-hawassa-ethiopia
#16
Biruhtesfa Atnafie, Degmawi Paulos, Mesele Abera, Genene Tefera, Dereje Hailu, Surafel Kasaye, Kebede Amenu
BACKGROUND: Despite of the sanitation measures in municipal abattoirs to reduce contamination, Escherichia coli continues to be a health hazard. The present study was conducted on 150 apparently healthy slaughtered cattle at municipal abattoir and in 50 different butcher shops in Hawassa town, Ethiopia. The objectives of the study were investigating the occurrence and antimicrobial resistance of E. coli O157:H7 isolated from fecal samples, carcasses swab, contacts surfaces (swabs of meat handlers hands, knife and clothes of meat transporters) as well as from butcher shops (meat samples, swabs from cutting board swab, butcher men hand and knife surface)...
January 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28109256/optimisation-of-methods-for-bacterial-skin-microbiome-investigation-primer-selection-and-comparison-of-the-454-versus-miseq-platform
#17
Madhura Castelino, Stephen Eyre, John Moat, Graeme Fox, Paul Martin, Pauline Ho, Mathew Upton, Anne Barton
BACKGROUND: The composition of the skin microbiome is predicted to play a role in the development of conditions such as atopic eczema and psoriasis. 16S rRNA gene sequencing allows the investigation of bacterial microbiota. A significant challenge in this field is development of cost effective high throughput methodologies for the robust interrogation of the skin microbiota, where biomass is low. Here we describe validation of methodologies for 16S rRNA (ribosomal ribonucleic acid) gene sequencing from the skin microbiome, using the Illumina MiSeq platform, the selection of primer to amplify regions for sequencing and we compare results with the current standard protocols...
January 21, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28103814/the-respiratory-microbiome-in-bronchial-mucosa-and-secretions-from-severe-ige-mediated-asthma-patients
#18
Laura Millares, Guadalupe Bermudo, Vicente Pérez-Brocal, Christian Domingo, Marian Garcia-Nuñez, Xavier Pomares, Andrés Moya, Eduard Monsó
BACKGROUND: The bronchial microbiome in chronic lung diseases presents an abnormal pattern, but its microbial composition and regional differences in severe asthma have not been sufficiently addressed. The aim of the study was to describe the bacterial community in bronchial mucosa and secretions of patients with severe chronic asthma chronically treated with corticosteroids in addition to usual care according to Global Initiative for Asthma. Bacterial community composition was obtained by 16S rRNA gene amplification and sequencing, and functional capabilities through PICRUSt...
January 19, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28103800/chmob2-binds-to-chcbk1-and-promotes-virulence-and-conidiation-of-the-fungal-pathogen-colletotrichum-higginsianum
#19
Johannes Schmidpeter, Marlis Dahl, Jörg Hofmann, Christian Koch
BACKGROUND: Mob family proteins are conserved between animals, plants and fungi and are essential for the activation of NDR kinases that control crucial cellular processes like cytokinesis, proliferation and morphology. RESULTS: We identified a hypomorphic allele of ChMOB2 in a random insertional mutant (vir-88) of the hemibiotrophic ascomycete fungus Colletotrichum higginsianum. The mutant is impaired in conidiation, host penetration and virulence on Arabidopsis thaliana...
January 19, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28103794/genomic-and-transcriptomic-comparison-between-staphylococcus-aureus-strains-associated-with-high-and-low-within-herd-prevalence-of-intra-mammary-infection
#20
E Capra, P Cremonesi, A Pietrelli, S Puccio, M Luini, A Stella, B Castiglioni
BACKGROUND: Staphylococcus aureus (Staph. aureus) is one of the major pathogens causing mastitis in dairy ruminants worldwide. The chronic nature of Staph. aureus infection enhances the contagiousness risk and diffusion in herds. In order to identify the factors involved in intra-mammary infection (IMI) and diffusion in dairy cows, we investigated the molecular characteristics of two groups of Staph. aureus strains belonging to ST8 and ST398, differing in clinical properties, through comparison of whole genome and whole transcriptome sequencing...
January 19, 2017: BMC Microbiology
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