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BMC Microbiology

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https://www.readbyqxmd.com/read/28645257/relationship-between-nasopharyngeal-and-bronchoalveolar-microbial-communities-in-clinically-healthy-feedlot-cattle
#1
Mohamed M Zeineldin, James F Lowe, Elsbeth D Grimmer, Maria R C de Godoy, Mohamed M Ghanem, Yassein M Abd El-Raof, Brian M Aldridge
BACKGROUND: The importance of upper airway structure in the susceptibility of the lower respiratory tract to colonization with potential pathogens is well established. With the advent of rapid, high throughput, next generation sequencing, there is a growing appreciation of the importance of commensal microbial populations in maintaining mucosal health, and a realization that bacteria colonize anatomical locations that were previously considered to be sterile. While upper respiratory tract microbial populations have been described, there are currently no published studies describing the normal microbial populations of the bovine lower respiratory tract...
June 23, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28637443/trichoderma-virens-%C3%AE-glucosidase-i-bgli-gene-expression-in-saccharomyces-cerevisiae-including-docking-and-molecular-dynamics-studies
#2
Gammadde Hewa Ishan Maduka Wickramasinghe, Pilimathalawe Panditharathna Attanayake Mudiyanselage Samith Indika Rathnayake, Naduviladath Vishvanath Chandrasekharan, Mahindagoda Siril Samantha Weerasinghe, Ravindra Lakshman Chundananda Wijesundera, Wijepurage Sandhya Sulochana Wijesundera
BACKGROUND: Cellulose, a linear polymer of β 1-4, linked glucose, is the most abundant renewable fraction of plant biomass (lignocellulose). It is synergistically converted to glucose by endoglucanase (EG) cellobiohydrolase (CBH) and β-glucosidase (BGL) of the cellulase complex. BGL plays a major role in the conversion of randomly cleaved cellooligosaccharides into glucose. As it is well known, Saccharomyces cerevisiae can efficiently convert glucose into ethanol under anaerobic conditions...
June 21, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28629317/surface-hypothermia-predicts-murine-mortality-in-the-intragastric-vibrio-vulnificus-infection-model
#3
Hannah E Gavin, Karla J F Satchell
BACKGROUND: The Gram-negative bacterium Vibrio vulnificus can cause severe disease in humans who consume undercooked, contaminated seafood. To study food-borne V. vulnificus disease in the laboratory, mouse virulence studies predominantly use death as the primary experimental endpoint because behaviorally based moribund status does not consistently predict lethality. This study assessed ventral surface temperature (VST) and its association with mouse survival during V. vulnificus virulence studies as an efficacious, humane alternative...
June 19, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28623898/early-life-gut-microbial-colonization-shapes-th1-th2-balance-in-asthma-model-in-balb-c-mice
#4
Li-Juan Qian, Shu-Min Kang, Jia-Li Xie, Li Huang, Quan Wen, Yuan-Yuan Fan, Li-Jun Lu, Li Jiang
BACKGROUND: We aimed to investigate the effect of early-life diverse microbial exposures on gut microbial colonization in an OVA-induced asthma model in BALB/c mice. METHODS: BALB/c mice were divided into 4 groups: A, offsprings were kept in a SPF environment during fetal, lactation, and childhood periods; B, offsprings were kept in the SPF environment during fetal and lactation periods, and kept in the general environment during childhood; C, offsprings were kept in the SPF environment only during fetal period, and then kept in the general environment; and D, offsprings were kept in the general environment during whole periods...
June 17, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28619007/whole-genome-sequencing-analyses-of-listeria-monocytogenes-that-persisted-in-a-milkshake-machine-for-a-year-and-caused-illnesses-in-washington-state
#5
Zhen Li, Ailyn Pérez-Osorio, Yu Wang, Kaye Eckmann, William A Glover, Marc W Allard, Eric W Brown, Yi Chen
BACKGROUND: In 2015, in addition to a United States multistate outbreak linked to contaminated ice cream, another outbreak linked to ice cream was reported in the Pacific Northwest of the United States. It was a hospital-acquired outbreak linked to milkshakes, made from contaminated ice cream mixes and milkshake maker, served to patients. Here we performed multiple analyses on isolates associated with this outbreak: pulsed-field gel electrophoresis (PFGE), whole genome single nucleotide polymorphism (SNP) analysis, species-specific core genome multilocus sequence typing (cgMLST), lineage-specific cgMLST and whole genome-specific MLST (wgsMLST)/outbreak-specific cgMLST...
June 15, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28595575/towards-diagnostic-metagenomics-of-campylobacter-in-fecal-samples
#6
Sandra Christine Andersen, Kristoffer Kiil, Christoffer Bugge Harder, Mathilde Hasseldam Josefsen, Søren Persson, Eva Møller Nielsen, Jeffrey Hoorfar
BACKGROUND: The development of diagnostic metagenomics is driven by the need for universal, culture-independent methods for detection and characterization of pathogens to substitute the time-consuming, organism-specific, and often culture-based laboratory procedures for epidemiological source-tracing. Some of the challenges in diagnostic metagenomics are, that it requires a great next-generation sequencing depth and unautomated data analysis. RESULTS: DNA from human fecal samples spiked with 7...
June 8, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28592242/an-avirulent-burkholderia-pseudomallei-%C3%A2-purm-strain-with-atypical-type-b-lps-expansion-of-the-toolkit-for-biosafe-studies-of-melioidosis
#7
Michael H Norris, Md Siddiqur Rahman Khan, Herbert P Schweizer, Apichai Tuanyok
BACKGROUND: The work was undertaken to expand the tools available for researching Burkholderia pseudomallei (Bp), the etiological agent of the tropical disease melioidosis. Melioidosis has the potential to pose a severe threat to public health and safety. In the United States, Bp is listed as a Tier-1 select agent by the Centers for Disease Control and Prevention (CDC), thus requiring high levels of regulation and biosafety level 3 (BSL3) facilities for experimental manipulation of live organisms...
June 7, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28583081/addition-of-plant-growth-promoting-bacillus-subtilis-pts-394-on-tomato-rhizosphere-has-no-durable-impact-on-composition-of-root-microbiome
#8
Junqing Qiao, Xiang Yu, Xuejie Liang, Yongfeng Liu, Rainer Borriss, Youzhou Liu
BACKGROUND: Representatives of the genus Bacillus are increasingly used in agriculture to promote plant growth and to protect against plant pathogens. Unfortunately, hitherto the impact of Bacillus inoculants on the indigenous plant microbiota has been investigated exclusively for the species Bacillus amyloliquefaciens and was limited to prokaryotes, whilst eukaryotic member of this community, e.g. fungi, were not considered. RESULTS: The root-colonizing Bacillus subtilis PTS-394 supported growth of tomato plants and suppressed soil-borne diseases...
June 5, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28577529/the-effect-of-immunoregulation-of-streptococcus-lactis-l16-strain-upon-staphylococcus-aureus-infection
#9
Maopeng Wang, Shengjie Gong, Shouwen Du, Yilong Zhu, Fengjun Rong, Rongrong Pan, Yang Di, Chang Li, Dayong Ren, Ningyi Jin
BACKGROUND: Staphylococcus aureus is an important pathogen that causes various infections in medical facilities. However, resistance to multiple drugs has made this infection difficult to manage. Thus, new therapeutic strategies are urgently needed to solve this worldwide public health problem. The Streptococcus lactis L16 strain was isolated from the fermented hot chili sauce. To explore whether it can be used as a protective agent against S. aureus infection, we designed a mouse model of S...
June 2, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28558650/isolation-characterization-and-transcriptome-analysis-of-a-novel-antarctic-aspergillus-sydowii-strain-ms-19-as-a-potential-lignocellulosic-enzyme-source
#10
Bailin Cong, Nengfei Wang, Shenghao Liu, Feng Liu, Xiaofei Yin, Jihong Shen
BACKGROUND: With the growing demand for fossil fuels and the severe energy crisis, lignocellulose is widely regarded as a promising cost-effective renewable resource for ethanol production, and the use of lignocellulose residues as raw material is remarkable. Polar organisms have important value in scientific research and development for their novelty, uniqueness and diversity. RESULTS: In this study, a fungus Aspergillus sydowii MS-19, with the potential for lignocellulose degradation was screened out and isolated from an Antarctic region...
May 30, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28545531/selection-and-characterization-of-alanine-racemase-inhibitors-against-aeromonas-hydrophila
#11
Yaping Wang, Chao Yang, Wen Xue, Ting Zhang, Xipei Liu, Jiansong Ju, Baohua Zhao, Dong Liu
BACKGROUND: Combining experimental and computational screening methods has been of keen interest in drug discovery. In the present study, we developed an efficient screening method that has been used to screen 2100 small-molecule compounds for alanine racemase Alr-2 inhibitors. RESULTS: We identified ten novel non-substrate Alr-2 inhibitors, of which patulin, homogentisic acid, and hydroquinone were active against Aeromonas hydrophila. The compounds were found to be capable of inhibiting Alr-2 to different extents with 50% inhibitory concentrations (IC50) ranging from 6...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28545528/comparison-of-biomarker-based-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-and-conventional-methods-in-the-identification-of-clinically-relevant-bacteria-and-yeast
#12
Ali Kassim, Valentin Pflüger, Zul Premji, Claudia Daubenberger, Gunturu Revathi
BACKGROUND: MALDI-TOF MS is an analytical method that has recently become integral in the identification of microorganisms in clinical laboratories. It relies on databases that majorly employ pattern recognition or fingerprinting. Biomarker based databases have also been developed and there is optimism that these may be superior to pattern recognition based databases. This study compared the performance of ribosomal biomarker based MALDI-TOF MS and conventional methods in the identification of selected bacteria and yeast...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28545489/cytotoxic-necrotizing-factor-1-cnf1-does-not-promote-e-coli-infection-in-a-murine-model-of-ascending-pyelonephritis
#13
Jason E Michaud, Kwang Sik Kim, William Harty, Matthew Kasprenski, Ming-Hsien Wang
BACKGROUND: Urinary tract infections (UTI) are among the most common and costly infections in both hospitalized and ambulatory patients. Uropathogenic E. coli (UPEC) represent the majority of UTI isolates and are a diverse group of bacteria that utilize a variety of virulence factors to establish infection of the genitourinary tract. The virulence factor cytotoxic necrotizing factor-1 (CNF1) is frequently expressed in clinical UPEC isolates. To date, there have been conflicting reports on the role of CNF1 in the pathogenesis of E...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28545480/effects-of-hepatitis-c-virus-core-protein-and-nonstructural-protein-4b-on-the-wnt-%C3%AE-catenin-pathway
#14
Xiao-Hua Jiang, Yu-Tao Xie, Ya-Ping Cai, Jing Ren, Tao Ma
BACKGROUND: Hepatitis C virus (HCV) core protein and nonstructural protein 4B (NS4B) are potentially oncogenic. Aberrant activation of the Wnt/β-catenin signaling pathway is closely associated with hepatocarcinogenesis. We investigated the effects of HCV type 1b core protein and NS4B on Wnt/β-catenin signaling in various liver cells, and explored the molecular mechanism underlying HCV-related hepatocarcinogenesis. RESULTS: Compared with the empty vector control, HCV core protein and NS4B demonstrated the following characteristics in the Huh7 cells: significantly enhanced β-catenin/Tcf-dependent transcriptional activity (F = 40...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28545445/identification-of-enterococcus-faecalis-enzymes-with-azoreductases-and-or-nitroreductase-activity
#15
Valérie Chalansonnet, Claire Mercier, Sylvain Orenga, Christophe Gilbert
BACKGROUND: Nitroreductases, NAD(P)H dependent flavoenzymes, are found in most of bacterial species. Even if Enterococcus faecalis strains seems to present such activity because of their sensitivity to nitrofurans, no enzyme has been described. Nitroreductases were separated of others reductases due to their capacity to reduce nitro compounds. They are further classified based on their preference in cofactor: NADH and/or NADPH. However, recently, azoreductases have been studied for their strong activity on nitro compounds, especially nitro pro-drugs...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28545444/site-specific-his-asp-phosphoproteomic-analysis-of-prokaryotes-reveals-putative-targets-for-drug-resistance
#16
Shu-Jung Lai, I-Fan Tu, Wan-Ling Wu, Jhih-Tian Yang, Louis Y P Luk, Mei-Chin Lai, Yu-Hsuan Tsai, Shih-Hsiung Wu
BACKGROUND: Phosphorylation of amino acid residues on proteins is an important and common post-translational modification in both eukaryotes and prokaryotes. Most research work has been focused on phosphorylation of serine, threonine or tyrosine residues, whereas phosphorylation of other amino acids are significantly less clear due to the controversy on their stability under standard bioanalytical conditions. RESULTS: Here we applied a shotgun strategy to analyze the histidine and aspartate phosphorylations in different microbes...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28545413/development-of-an-lps-based-elisa-for-diagnosis-of-yersinia-enterocolitica-o-3-infections-in-danish-patients-a-follow-up-study
#17
Tine Dalby, Eva Rasmussen, Peter Schiellerup, Karen Angeliki Krogfelt
BACKGROUND: The bacterium Yersinia enterocolitica causes gastroenteritis in humans. The study aimed to develop a diagnostic enzyme-linked immunosorbent assay (ELISA) for detection of Yersinia enterocolitica O:3 LPS antibodies in sera from Danish patients with suspected Yersinia enterocolitica O:3 gastrointestinal infection. As a part of this, antibody decay profiles after culture confirmed Yersinia enteritis were studied. RESULTS: An ELISA using Yersinia enterocolitica O:3 LPS as the coating antigen was developed for measuring IgA, IgG and IgM specific antibodies...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28535747/in-vitro-assessment-of-pediococcus-acidilactici-kp10-for-its-potential-use-in-the-food-industry
#18
Sahar Abbasiliasi, Joo Shun Tan, Fatemeh Bashokouh, Tengku Azmi Tengku Ibrahim, Shuhaimi Mustafa, Faezeh Vakhshiteh, Subhashini Sivasamboo, Arbakariya B Ariff
BACKGROUND: Selection of a microbial strain for the incorporation into food products requires in vitro and in vivo evaluations. A bacteriocin-producing lactic acid bacterium (LAB), Pediococcus acidilactici Kp10, isolated from a traditional dried curd was assessed in vitro for its beneficial properties as a potential probiotic and starter culture. The inhibitory spectra of the bacterial strain against different gram-positive and gram-negative bacteria, its cell surface hydrophobicity and resistance to phenol, its haemolytic, amylolytic and proteolytic activities, ability to produce acid and coagulate milk together with its enzymatic characteristics and adhesion property were all evaluated in vitro...
May 23, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28532414/association-between-body-mass-index-and-firmicutes-bacteroidetes-ratio-in-an-adult-ukrainian-population
#19
Alexander Koliada, Ganna Syzenko, Vladislav Moseiko, Liudmyla Budovska, Kostiantyn Puchkov, Vyacheslav Perederiy, Yuriy Gavalko, Andriy Dorofeyev, Maryana Romanenko, Sergiy Tkach, Lyudmila Sineok, Oleh Lushchak, Alexander Vaiserman
BACKGROUND: Metagenomic studies confirm that obesity is associated with a composition of gut microbiota. There are some controversies, however, about the composition of gut microbial communities in obese individuals in different populations. To examine the association between body mass index and microbiota composition in Ukrainian population, fecal concentrations of Bacteroidetes, Firmicutes, Actinobacteria and Firmicutes/Bacteroidetes (F/B) ratio were analyzed in 61 adult individuals...
May 22, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28532385/erratum-to-the-holliday-junction-resolvase-recu-is-required-for-chromosome-segregation-and-dna-damage-repair-in-staphylococcus-aureus
#20
Ana R Pereira, Patricia Reed, Helena Veiga, Mariana G Pinho
No abstract text is available yet for this article.
May 22, 2017: BMC Microbiology
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