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BMC Microbiology

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https://www.readbyqxmd.com/read/28915790/the-process-related-dynamics-of-microbial-community-during-a-simulated-fermentation-of-chinese-strong-flavored-liquor
#1
Yanyan Zhang, Xiaoyu Zhu, Xiangzhen Li, Yong Tao, Jia Jia, Xiaohong He
BACKGROUND: Famous Chinese strong-flavored liquor (CSFL) is brewed by microbial consortia in a special fermentation pit (FT). However, the fermentation process was not fully understood owing to the complicate community structure and metabolism. In this study, the process-related dynamics of microbial communities and main flavor compounds during the 70-day fermentation process were investigated in a simulated fermentation system. RESULTS: A three-phase model was proposed to characterize the process of the CSFL fermentation...
September 15, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28903732/a-comparison-of-sequencing-platforms-and-bioinformatics-pipelines-for-compositional-analysis-of-the-gut-microbiome
#2
Imane Allali, Jason W Arnold, Jeffrey Roach, Maria Belen Cadenas, Natasha Butz, Hosni M Hassan, Matthew Koci, Anne Ballou, Mary Mendoza, Rizwana Ali, M Andrea Azcarate-Peril
BACKGROUND: Advancements in Next Generation Sequencing (NGS) technologies regarding throughput, read length and accuracy had a major impact on microbiome research by significantly improving 16S rRNA amplicon sequencing. As rapid improvements in sequencing platforms and new data analysis pipelines are introduced, it is essential to evaluate their capabilities in specific applications. The aim of this study was to assess whether the same project-specific biological conclusions regarding microbiome composition could be reached using different sequencing platforms and bioinformatics pipelines...
September 13, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28903728/increased-survival-and-proliferation-of-the-epidemic-strain-mycobacterium-abscessus-subsp-massiliense-crm0019-in-alveolar-epithelial-cells
#3
Giovanni Monteiro Ribeiro, Cristianne Kayoko Matsumoto, Fernando Real, Daniela Teixeira, Rafael Silva Duarte, Renato Arruda Mortara, Sylvia Cardoso Leão, Cristiane de Souza Carvalho-Wodarz
BACKGROUND: Outbreaks of infections caused by rapidly growing mycobacteria have been reported worldwide generally associated with medical procedures. Mycobacterium abscessus subsp. massiliense CRM0019 was obtained during an epidemic of postsurgical infections and was characterized by increased persistence in vivo. To better understand the successful survival strategies of this microorganism, we evaluated its infectivity and proliferation in macrophages (RAW and BMDM) and alveolar epithelial cells (A549)...
September 13, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28899359/transcript-profiling-of-the-immunological-interactions-between-actinobacillus-pleuropneumoniae-serotype-7-and-the-host-by-dual-rna-seq
#4
Ping Li, Zhiwen Xu, Xiangang Sun, Yue Yin, Yi Fan, Jun Zhao, Xiyu Mao, Jianbo Huang, Fan Yang, Ling Zhu
BACKGROUND: The complexity of the pathogenic mechanism underlying the host immune response to Actinobacillus pleuropneumonia (App) makes the use of preventive measures difficult, and a more global view of the host-pathogen interactions and new insights into this process are urgently needed to reveal the pathogenic and immune mechanisms underlying App infection. Here, we infected specific pathogen-free Mus musculus with App serotype 7 by intranasal inoculation to construct an acute hemorrhagic pneumonia infection model and isolated the infected lungs for analysis of the interactions by dual RNA-seq...
September 12, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28899339/ciliate-diversity-and-distribution-patterns-in-the-sediments-of-a-seamount-and-adjacent-abyssal-plains-in-the-tropical-western-pacific-ocean
#5
Feng Zhao, Sabine Filker, Thorsten Stoeck, Kuidong Xu
BACKGROUND: Benthic ciliates and the environmental factors shaping their distribution are far from being completely understood. Likewise, deep-sea systems are amongst the least understood ecosystems on Earth. In this study, using high-throughput DNA sequencing, we investigated the diversity and community composition of benthic ciliates in different sediment layers of a seamount and an adjacent abyssal plain in the tropical Western Pacific Ocean with water depths ranging between 813 m and 4566 m...
September 12, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28893180/annexin-a2-anxa2-interacts-with-nonstructural-protein-1-and-promotes-the-replication-of-highly-pathogenic-h5n1-avian-influenza-virus
#6
Yong Ma, Jiashan Sun, Linlin Gu, Hongmei Bao, Yuhui Zhao, Lin Shi, Wei Yao, Guobin Tian, Xiurong Wang, Hualan Chen
BACKGROUND: Non-structural protein 1 (NS1) is a multifunctional protein and a crucial regulatory factor in the replication and pathogenesis of avian influenza virus (AIV). Studies have shown that NS1 can interact with a variety of host proteins to modulate the viral life cycle. We previously generated a monoclonal antibody against NS1 protein; In the current research study, using this antibody, we immunoprecipitated host proteins that interact with NS1 to better understand the roles played by NS1 in communications between virus and host...
September 11, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28854878/comparison-of-rumen-bacterial-communities-in-dairy-herds-of-different-production
#7
Nagaraju Indugu, Bonnie Vecchiarelli, Linda D Baker, James D Ferguson, Jairam K P Vanamala, Dipti W Pitta
BACKGROUND: The purpose of this study was to compare the rumen bacterial composition in high and low yielding dairy cows within and between two dairy herds. Eighty five Holstein dairy cows in mid-lactation (79-179 days in milk) were selected from two farms: Farm 12 (M305 = 12,300 kg; n = 47; 24 primiparous cows, 23 multiparous cows) and Farm 9 (M305 = 9700 kg; n = 38; 19 primiparous cows, 19 multiparous cows). Each study cow was sampled once using the stomach tube method and processed for 16S rRNA gene amplicon sequencing using the Ion Torrent (PGM) platform...
August 30, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28851288/evaluation-of-microplate-immunocapture-method-for-detection-of-vibrio-cholerae-salmonella-typhi-and-shigella-flexneri-from-food
#8
Md Fakruddin, Md Nur Hossain, Monzur Morshed Ahmed
BACKGROUND: Improved methods with better separation and concentration ability for detection of foodborne pathogens are in constant need. The aim of this study was to evaluate microplate immunocapture (IC) method for detection of Salmonella Typhi, Shigella flexneri and Vibrio cholerae from food samples to provide a better alternative to conventional culture based methods. RESULTS: The IC method was optimized for incubation time, bacterial concentration, and capture efficiency...
August 29, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28851281/growth-phase-dependent-expression-profiles-of-three-vital-h-ns-family-proteins-encoded-on-the-chromosome-of-pseudomonas-putida-kt2440-and-on-the-pcar1-plasmid
#9
Zongping Sun, Delyana Vasileva, Chiho Suzuki-Minakuchi, Kazunori Okada, Feng Luo, Yasuo Igarashi, Hideaki Nojiri
BACKGROUND: H-NS family proteins are nucleoid-associated proteins that form oligomers on DNA and function as global regulators. They are found in both bacterial chromosomes and plasmids, and were suggested to be candidate effectors of the interaction between them. TurA and TurB are the predominantly expressed H-NS family proteins encoded on the chromosome of Pseudomonas putida KT2440, while Pmr is encoded on the carbazole-degradative incompatibility group P-7 plasmid pCAR1. Previous transcriptome analyses suggested that they function cooperatively, but play different roles in the global transcriptional network...
August 29, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28836948/il-8-and-ip-10-expression-from-human-bronchial-epithelial-cells-beas-2b-are-promoted-by-streptococcus-pneumoniae-endopeptidase-o-pepo
#10
Jiaqiong Zou, Long Zhou, Chunlan Hu, Peng Jing, Xiaolan Guo, Sulan Liu, Yan Lei, Shangyu Yang, Jiankang Deng, Hong Zhang
BACKGROUND: The bronchial epithelium serves as the first defendant line of host against respiratory inhaled pathogens, mainly through releasing chemokines (e.g. interleukin-8 (IL-8), interferon-induced protein 10 (IP-10) etc.) responsible for neutrophil or lymphocyte recruitment to promote the clearance of inhaled pathogens including Streptococcus pneumoniae (S. pneumoniae). Previous studies have shown that IL-8 expression is induced by pneumococcal virulence factors (e.g. pneumolysin, peptidoglycan-polysaccharides, pneumococcal surface protein A (PspA) etc...
August 24, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28835204/erratum-to-the-photosensor-protein-ppr-of-rhodocista-centenaria-is-linked-to-the-chemotaxis-signalling-pathway
#11
Sven Kreutel, Andreas Kuhn, Dorothee Kiefer
No abstract text is available yet for this article.
August 23, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28835202/upper-and-lower-respiratory-tract-microbiota-in-horses-bacterial-communities-associated-with-health-and-mild-asthma-inflammatory-airway-disease-and-effects-of-dexamethasone
#12
Stephanie L Bond, Edouard Timsit, Matthew Workentine, Trevor Alexander, Renaud Léguillette
BACKGROUND: The microbial composition of the equine respiratory tract, and differences due to mild equine asthma (also called Inflammatory Airway Disease (IAD)) have not been reported. The primary treatment for control of IAD in horses are corticosteroids. The objectives were to characterize the upper and lower respiratory tract microbiota associated with respiratory health and IAD, and to investigate the effects of dexamethasone on these bacterial communities using high throughput sequencing...
August 23, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28835201/irak-m-alters-the-polarity-of-macrophages-to-facilitate-the-survival-of-mycobacterium-tuberculosis
#13
Pei Shen, Quan Li, Jilei Ma, Maopeng Tian, Fei Hong, Xinjie Zhai, Jianrong Li, Hanju Huang, Chunwei Shi
BACKGROUND: Intracellular bacterium, Mycobacterium tuberculosis (M. tb), infects specifically macrophages as host cells. IRAK-M, a member of IRAK family, is a negative regulator in TLR signaling and specifically expresses in monocytes and macrophages. The role of IRAK-M in intracellular growth of M. tb and macrophage polarization was explored, for deeply understanding the pathogenesis of M. tb, the significance of IRAK-M to innate immunity and pathogen-host interaction. METHODS: IRAK-M expression was detected in M...
August 23, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28830351/genome-sequencing-and-comparative-genomics-of-enterohemorrhagic-escherichia-coli-o145-h25-and-o145-h28-reveal-distinct-evolutionary-paths-and-marked-variations-in-traits-associated-with-virulence-colonization
#14
Sandra C Lorenz, Narjol Gonzalez-Escalona, Michael L Kotewicz, Markus Fischer, Julie A Kase
BACKGROUND: Enterohemorrhagic Escherichia coli (EHEC) O145 are among the top non-O157 serogroups associated with severe human disease worldwide. Two serotypes, O145:H25 and O145:H28 have been isolated from human patients but little information is available regarding the virulence repertoire, origin and evolutionary relatedness of O145:H25. Hence, we sequenced the complete genome of two O145:H25 strains associated with hemolytic uremic syndrome (HUS) and compared the genomes with those of previously sequenced O145:H28 and other EHEC strains...
August 22, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28830348/evaluation-of-trypan-blue-stain-in-a-haemocytometer-for-rapid-detection-of-cerebrospinal-fluid-sterility-in-hiv-patients-with-cryptococcal-meningitis
#15
Richard Kwizera, Andrew Akampurira, Tadeo K Kandole, Kirsten Nielsen, Andrew Kambugu, David B Meya, David R Boulware, Joshua Rhein
BACKGROUND: Quantitative culture is the most common method to determine the fungal burden and sterility of cerebrospinal fluid (CSF) among persons with cryptococcal meningitis. A major drawback of cultures is a long turnaround-time. Recent evidence demonstrates that live and dead Cryptococcus yeasts can be distinguished using trypan blue staining. We hypothesized that trypan blue staining combined with haemocytometer counting may provide a rapid estimation of quantitative culture count and detection of CSF sterility...
August 22, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28830344/tum1-is-involved-in-the-metabolism-of-sterol-esters-in-saccharomyces-cerevisiae
#16
Katja Uršič, Mojca Ogrizović, Dušan Kordiš, Klaus Natter, Uroš Petrovič
BACKGROUND: The only hitherto known biological role of yeast Saccharomyces cerevisiae Tum1 protein is in the tRNA thiolation pathway. The mammalian homologue of the yeast TUM1 gene, the thiosulfate sulfurtransferase (a.k.a. rhodanese) Tst, has been proposed as an obesity-resistance and antidiabetic gene. To assess the role of Tum1 in cell metabolism and the putative functional connection between lipid metabolism and tRNA modification, we analysed evolutionary conservation of the rhodanese protein superfamily, investigated the role of Tum1 in lipid metabolism, and examined the phenotype of yeast strains expressing the mouse homologue of Tum1, TST...
August 22, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28826394/erratum-to-membrane-insertion-and-assembly-of-epitope-tagged-gp9-at-the-tip-of-the-m13-phage
#17
Martin Ploss, Andreas Kuhn
No abstract text is available yet for this article.
August 21, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28821241/genetic-diversity-and-virulence-potential-of-clinical-and-environmental-aeromonas-spp-isolates-from-a-diarrhea-outbreak
#18
Lívia Christina Alves da Silva, Tereza Cristina Leal-Balbino, Beatriz Souza Toscano de Melo, Carina Lucena Mendes-Marques, Antonio Mauro Rezende, Alzira Maria Paiva de Almeida, Nilma Cintra Leal
BACKGROUND: Aeromonas spp. are gram-negative bacteria that can cause a variety of infections in both humans and animals and play a controversial role in diarrhea outbreaks. Our aim was to identify clinical and environmental Aeromonas isolates associated with a cholera outbreak in a northeast county of Brazil at the species level. We also aimed to determine the genetic structure of the bacterial population and the virulence potential of the Aeromonas isolates. METHODS AND RESULTS: Analysis based on concatenated sequences of the 16S rRNA and gyrB genes suggested the classification of the 119 isolates studied into the following species: A...
August 18, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28821225/differences-in-survival-virulence-and-biofilm-formation-between-sialidase-deficient-and-w83-wild-type-porphyromonas-gingivalis-strains-under-stressful-environmental-conditions
#19
Xiaoyu Xu, Tong Tong, Xue Yang, Yaping Pan, Li Lin, Chen Li
BACKGROUND: Porphyromonas gingivalis is a major causative pathogen of chronic periodontitis. Within the inflammatory microenvironment, there exists extreme pH values, elevated temperatures and oxidative stress. Pathogens adapt to these stressful environmental conditions by regulating the transcription of virulence genes, modifying themselves with macromolecules and by aggregating and entering into a biofilm growth phase. Our previous study showed that the P. gingivalis sialidase can help cells obtain sialic acid from the environment, which is used to modify macromolecules on the surface of P...
August 18, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28821221/acute-penicillium-marneffei-infection-stimulates-host-m1-m2a-macrophages-polarization-in-balb-c-mice
#20
Xiaoying Dai, Congzheng Mao, Xiuwan Lan, Huan Chen, Meihua Li, Jing Bai, Jingmin Deng, Qiuli Liang, Jianquan Zhang, Xiaoning Zhong, Yi Liang, Jiangtao Fan, Honglin Luo, Zhiyi He
BACKGROUND: Penicillium marneffei (P. marneffei) is a thermally dimorphic fungus pathogen that causes fatal infection. Alveolar macrophages are innate immune cells that have critical roles in protection against pulmonary fungal pathogens and the macrophage polarization state has the potential to be a deciding factor in disease progression or resolution. The aim of this study was to investigate mouse alveolar macrophage polarization states during P. marneffei infection. RESULTS: We used enzyme-linked immunosorbent (ELISA) assays, quantitative real-time PCR (qRT-PCR), and Griess, arginase activity to evaluate the phenotypic markers of alveolar macrophages from BALB/C mice infected with P...
August 18, 2017: BMC Microbiology
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