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BMC Microbiology

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https://www.readbyqxmd.com/read/28525986/the-lspc3-41i-restriction-modification-system-is-the-major-determinant-for-genetic-manipulations-of-lysinibacillus-sphaericus-c3-41
#1
Pan Fu, Yong Ge, Yiming Wu, Ni Zhao, Zhiming Yuan, Xiaomin Hu
BACKGROUND: Lysinibacillus sphaericus has been widely used in integrated mosquito control program and it is one of the minority bacterial species unable to metabolize carbohydrates. In consideration of the high genetic conservation at genomic level and difficulty of genetic horizontal transfer, it is hypothesized that effective restriction-modification (R-M) systems existed in mosquitocidal L. sphaericus. RESULTS: In this study, six type II R-M systems including LspC3-41I were predicted in L...
May 19, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28525985/investigation-of-optimum-ohmic-heating-conditions-for-inactivation-of-escherichia-coli-o157-h7-salmonella-enterica-serovar-typhimurium-and-listeria-monocytogenes-in-apple-juice
#2
Il-Kyu Park, Jae-Won Ha, Dong-Hyun Kang
BACKGROUND: Control of foodborne pathogens is an important issue for the fruit juice industry and ohmic heating treatment has been considered as one of the promising antimicrobial interventions. However, to date, evaluation of the relationship between inactivation of foodborne pathogens and system performance efficiency based on differing soluble solids content of apple juice during ohmic heating treatment has not been well studied. This study aims to investigate effective voltage gradients of an ohmic heating system and corresponding sugar concentrations (°Brix) of apple juice for inactivating major foodborne pathogens (E...
May 19, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28525966/gene-expression-of-vibrio-parahaemolyticus-growing-in-laboratory-isolation-conditions-compared-to-those-common-in-its-natural-ocean-environment
#3
Katherine García, Cristian Yáñez, Nicolás Plaza, Francisca Peña, Pedro Sepúlveda, Diliana Pérez-Reytor, Romilio T Espejo
BACKGROUND: Vibrio parahaemolyticus is an autochthonous marine bacterial species comprising strains able to grow in broth containing bile salts at 37 °C, a condition seldom found in the ocean. However, this condition is used for isolation in the laboratory because it is considered a necessary property for pathogenesis. In this context, revealing how gene expression enables V. parahaemolyticus to adapt to this particular condition -common to almost all V. parahaemolyticus isolates- will improve our understanding of the biology of this important pathogen...
May 19, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28506316/characterization-of-novel-omp31-antigenic-epitopes-of-brucella-melitensis-by-monoclonal-antibodies
#4
Jinfeng Li, Feihuan Hu, Shouyi Chen, Peifang Luo, Zuoping He, Wenjing Wang, Jean-Pierre Allain, Chengyao Li
BACKGROUND: Brucellosis is a severe zoonotic disease worldwide. Detection and identification of Brucella species are essential to prevent or treat brucellosis in humans and animals. The outer membrane protein-31 (Omp31) is a major protein of Brucellae except for B. abortus, while the Omp31 antigenic epitopes have not been extensively characterized yet. RESULTS: A total of 22 monoclonal antibodies (mAbs) were produced against Omp31 of Brucella (B.) melitensis, of which 13 recognized five linear epitopes, 7 reacted with semi-conformational epitopes and 2 reacted with conformational epitopes, respectively...
May 15, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28506262/identification-and-quantification-of-virulence-factors-of-enterotoxigenic-escherichia-coli-by-high-resolution-melting-curve-quantitative-pcr
#5
Weilan Wang, Ruurd T Zijlstra, Michael G Gänzle
BACKGROUND: Diagnosis of enterotoxigenic E. coli (ETEC) associated diarrhea is complicated by the diversity of E.coli virulence factors. This study developed a multiplex quantitative PCR assay based on high-resolution melting curves analysis (HRM-qPCR) to identify and quantify genes encoding five ETEC fimbriae related to diarrhea in swine, i.e. K99, F41, F18, F6 and K88. METHODS: Five fimbriae expressed by ETEC were amplified in multiple HRM-qPCR reactions to allow simultaneous identification and quantification of five target genes...
May 15, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28506251/diversity-in-vitro-virulence-traits-and-antifungal-susceptibility-pattern-of-gastrointestinal-yeast-flora-of-healthy-poultry-gallus-gallus-domesticus
#6
Supram Hosuru Subramanya, Nawal Kishor Sharan, Bharat Prasad Baral, Deependra Hamal, Niranjan Nayak, Peralam Yegneswaran Prakash, Brijesh Sathian, Indira Bairy, Shishir Gokhale
BACKGROUND: Poultry farming and consumption of poultry (Gallus gallus domesticus) meat and eggs are common gastronomical practices worldwide. Till now, a detailed understanding about the gut colonisation of Gallus gallus domesticus by yeasts and their virulence properties and drug resistance patterns in available literature remain sparse. This study was undertaken to explore this prevalent issue. RESULTS: A total of 103 specimens of fresh droppings of broiler chickens (commercial G domesticus) and domesticated chickens (domesticated G domesticus) were collected from the breeding sites...
May 15, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28499353/transposon-insertion-sequencing-reveals-t4ss-as-the-major-genetic-trait-for-conjugation-transfer-of-multi-drug-resistance-peib202-from-edwardsiella
#7
Yang Liu, Yanan Gao, Xiaohong Liu, Qin Liu, Yuanxing Zhang, Qiyao Wang, Jingfan Xiao
BACKGROUND: Conjugation is a major type of horizontal transmission of genes that involves transfer of a plasmid into a recipient using specific conjugation machinery, which results in an extended spectrum of bacterial antibiotics resistance. However, there is inadequate knowledge about the regulator and mechanisms that control the conjugation processes, especially in an aquaculture environment where a cocktail of antibiotics may be present. Here, we investigated these with pEIB202, a typical multi-drug resistant IncP plasmid encoding tetracycline, streptomycin, sulfonamide and chloramphenicol resistance in fish pathogen Edwardsiella piscicida strain EIB202...
May 12, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28494766/filmarray%C3%A2-gi-panel-performance-for-the-diagnosis-of-acute-gastroenteritis-or-hemorragic-diarrhea
#8
Antonio Piralla, Giovanna Lunghi, Gianluigi Ardissino, Alessia Girello, Marta Premoli, Erika Bava, Milena Arghittu, Maria Rosaria Colombo, Alessandra Cognetto, Patrizia Bono, Giulia Campanini, Piero Marone, Fausto Baldanti
BACKGROUND: Acute gastroenteritis is a common cause of morbidity and mortality in humans worldwide. The rapid and specific identification of infectious agents is crucial for correct patient management. However, diagnosis of acute gastroenteritis is usually performed with diagnostic panels that include only a few pathogens. In the present bicentric study, the diagnostic value of FilmArray™ GI panels was assessed in unformed stool samples of patients with acute gastroenteritis and in a series of samples collected from pediatric patients with heamorragic diarrhea...
May 12, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28490368/erratum-to-genomic-characterization-of-plant-cell-wall-degrading-enzymes-and-in-silico-analysis-of-xylanases-and-polygalacturonases-of-fusarium-virguliforme
#9
Hao-Xun Chang, Craig R Yendrek, Gustavo Caetano-Anolles, Glen L Hartman
No abstract text is available yet for this article.
May 10, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28486933/influence-of-chronic-azithromycin-treatment-on-the-composition-of-the-oropharyngeal-microbial-community-in-patients-with-severe-asthma
#10
Guido Lopes Dos Santos Santiago, Guy Brusselle, Kenny Dauwe, Pieter Deschaght, Chris Verhofstede, Dries Vaneechoutte, Ellen Deschepper, Paul Jordens, Guy Joos, Mario Vaneechoutte
BACKGROUND: This study of the oropharyngeal microbiome complements the previously published AZIthromycin in Severe ASThma (AZISAST) clinical trial, where the use of azithromycin was assessed in subjects with exacerbation-prone severe asthma. Here, we determined the composition of the oropharyngeal microbial community by means of deep sequencing of the amplified 16S rRNA gene in oropharyngeal swabs from patients with exacerbation-prone severe asthma, at baseline and during and after 6 months treatment with azithromycin or placebo...
May 10, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28477627/in-vitro-and-in-vivo-antagonistic-activity-of-new-probiotic-culture-against-clostridium-difficile-and-clostridium-perfringens
#11
Nataša Golić, Katarina Veljović, Nikola Popović, Jelena Djokić, Ivana Strahinić, Igor Mrvaljević, Amarela Terzić-Vidojević
BACKGROUND: Genus Clostridium accompanies more than 200 known species and at least 30 among them are associated with human and animal diseases. At the moment, the treatment of clostridial infections is based on use of antibiotics. However, due to the European ban on the use of antibiotics in livestock production, novel therapeutic strategies for treatment of these hardly curable infections have been evaluated. Hence, in this study the antimicrobial effect of newly designed probiotic culture consisted of natural isolates Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44 against Clostridium difficile and Clostridium perfringens was analyzed...
May 6, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28477626/when-more-is-less-emergent-suppressive-interactions-in-three-drug-combinations
#12
Casey Beppler, Elif Tekin, Cynthia White, Zhiyuan Mao, Jeffrey H Miller, Robert Damoiseaux, Van M Savage, Pamela J Yeh
BACKGROUND: In drug-drug interactions, there are surprising cases in which the growth inhibition of bacteria by a single antibiotic decreases when a second antibiotic is added. These interactions are termed suppressive and have been argued to have the potential to limit the evolution of resistance. Nevertheless, little attention has been given to suppressive interactions because clinical studies typically search for increases in killing efficiency and because suppressive interactions are believed to be rare based on pairwise studies...
May 6, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28476097/colonization-patterns-of-enterococcus-cecorum-in-two-different-broiler-production-cycles-detected-with-a-newly-developed-quantitative-real-time-pcr
#13
Arne Jung, Henning Petersen, Lydia Teske, Silke Rautenschlein
BACKGROUND: Although Enterococcus cecorum (EC) infection is one of the most important bacterial diseases in modern broiler chickens today, many aspects of epidemiology and pathogenesis are still unknown. There is a need for better detection methods for EC than classical cultivation. In the present study, we describe the validation and application of a newly developed quantitative TaqMan real-time PCR (qPCR) assay based on the 16S-rRNA-gene for the detection of EC. RESULTS: Fifty EC strains isolated from 12 different animal species were detected with the assay, while none of the other 26 examined bacterial species were tested positive during validation procedure...
May 5, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28468636/nationwide-molecular-epidemiology-of-methicillin-resistant-staphylococcus-aureus-responsible-for-horse-infections-in-france
#14
François Guérin, Marguerite Fines-Guyon, Pierrick Meignen, Géraldine Delente, Caroline Fondrinier, Nancy Bourdon, Vincent Cattoir, Albertine Léon
BACKGROUND: The epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) isolated in horse infections is not well documented, especially in France. The aim of the study was to evaluate the prevalence of MRSA isolates in horse infections from 2007 to 2013 in France and to characterize phenotypically and genotypically this collection. RESULTS: Out of 1393 S. aureus horse isolates, 85 (6.1%) were confirmed to be MRSA. Interestingly, the prevalence of MRSA significantly increased from 2007-2009 to 2010-2013 (0...
May 3, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28468604/simultaneous-detection-of-chikungunya-virus-dengue-virus-and-human-pathogenic-leptospira-genomes-using-a-multiplex-taqman%C3%A2-assay
#15
Claude Giry, Bénédicte Roquebert, Ghislaine Li-Pat-Yuen, Philippe Gasque, Marie-Christine Jaffar-Bandjee
BACKGROUND: In 2005-2006 a major epidemics of Chikungunya disease occurred in South-West Indian Ocean islands. In Reunion Island, the magnitude of Chikungunya infection related symptoms was high and with over 38% of serological prevalence in the population. This epidemics illustrated the potential threat of emerging arboviral diseases for inhabitants of Reunion Island and elsewhere since vectors are worldwide distributed. A sentinel surveillance network was set-up to detect emerging pathogens associated with fever over 38 °C and in the absence of known etiologic causes...
May 3, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28464816/identification-and-sequence-analyses-of-novel-lipase-encoding-novel-thermophillic-bacilli-isolated-from-armenian-geothermal-springs
#16
Grigor Shahinyan, Armine Margaryan, Hovik Panosyan, Armen Trchounian
BACKGROUND: Among the huge diversity of thermophilic bacteria mainly bacilli have been reported as active thermostable lipase producers. Geothermal springs serve as the main source for isolation of thermostable lipase producing bacilli. Thermostable lipolytic enzymes, functioning in the harsh conditions, have promising applications in processing of organic chemicals, detergent formulation, synthesis of biosurfactants, pharmaceutical processing etc. RESULTS: In order to study the distribution of lipase-producing thermophilic bacilli and their specific lipase protein primary structures, three lipase producers from different genera were isolated from mesothermal (27...
May 2, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28449650/structure-genetics-and-worldwide-spread-of-new-delhi-metallo-%C3%AE-lactamase-ndm-a-threat-to-public-health
#17
REVIEW
Asad U Khan, Lubna Maryam, Raffaele Zarrilli
BACKGROUND: The emergence of carbapenemase producing bacteria, especially New Delhi metallo-β-lactamase (NDM-1) and its variants, worldwide, has raised amajor public health concern. NDM-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of infections caused by resistant strain of bacteria. MAIN BODY: In this review, we have discussed bla NDM-1variants, its genetic analysis including type of specific mutation, origin of country and spread among several type of bacterial species...
April 27, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28449644/erratum-to-the-altered-gut-microbiota-in-adults-with-cystic-fibrosis
#18
D G Burke, F Fouhy, M J Harrison, M C Rea, P D Cotter, O O'Sullivan, C Stanton, C Hill, F Shanahan, B J Plant, R P Ross
No abstract text is available yet for this article.
April 27, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28441930/ribosomal-subunit-protein-typing-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-for-the-identification-and-discrimination-of-aspergillus-species
#19
Sayaka Nakamura, Hiroaki Sato, Reiko Tanaka, Yoko Kusuya, Hiroki Takahashi, Takashi Yaguchi
BACKGROUND: Accurate identification of Aspergillus species is a very important subject. Mass spectral fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is generally employed for the rapid identification of fungal isolates. However, the results are based on simple mass spectral pattern-matching, with no peak assignment and no taxonomic input. We propose here a ribosomal subunit protein (RSP) typing technique using MALDI-TOF MS for the identification and discrimination of Aspergillus species...
April 26, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28438125/chlamydia-spp-development-is-differentially-altered-by-treatment-with-the-lpxc-inhibitor-lpc-011
#20
Erik D Cram, Daniel D Rockey, Brian P Dolan
BACKGROUND: Chlamydia species are obligate intracellular bacteria that infect a broad range of mammalian hosts. Members of related genera are pathogens of a variety of vertebrate and invertebrate species. Despite the diversity of Chlamydia, all species contain an outer membrane lipooligosaccharide (LOS) that is comprised of a genus-conserved, and genus-defining, trisaccharide 3-deoxy-D-manno-oct-2-ulosonic acid Kdo region. Recent studies with lipopolysaccharide inhibitors demonstrate that LOS is important for the C...
April 24, 2017: BMC Microbiology
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