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BMC Microbiology

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https://www.readbyqxmd.com/read/28431522/comparative-proteomic-analysis-of-neisseria-meningitidis-wildtype-and-dpra-null-mutant-strains-links-dna-processing-to-pilus-biogenesis
#1
Getachew Tesfaye Beyene, Shewit Kalayou, Tahira Riaz, Tone Tonjum
BACKGROUND: DNA processing chain A (DprA) is a DNA binding protein which is ubiquitous in bacteria, and is required for DNA transformation to various extents among bacterial species. However, the interaction of DprA with competence and recombination proteins is poorly understood. Therefore, the proteomes of whole Neisseria meningitidis (Nm) wildtype and dprA mutant cells were compared. Such a comparative proteomic analysis increases our understanding of the interactions of DprA with other Nm components and may elucidate its potential role beyond DNA processing in transformation...
April 21, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28431497/effect-of-ensiled-mulberry-leaves-and-sun-dried-mulberry-fruit-pomace-on-the-fecal-bacterial-community-composition-in-finishing-steers
#2
Yan Li, Qingxiang Meng, Bo Zhou, Zhenming Zhou
BACKGROUND: Here, we aimed to investigate the effects of ensiled mulberry leaves (EML) and sun-dried mulberry fruit pomace (SMFP) on fecal bacterial communities in Simmental crossbred finishing steers. To this end, the steers were reared on a standard TMR diet, standard diet containing EML, and standard diet containing SMFP. The protein and energy levels of all the diets were similar. Illumina MiSeq sequencing of the V4 region of the 16S rRNA gene and quantitative real-time PCR were used to analyze and detect the fecal bacterial community...
April 21, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28427348/comparative-genomics-and-evolution-of-the-amylase-binding-proteins-of-oral-streptococci
#3
Elaine M Haase, Yurong Kou, Amarpreet Sabharwal, Yu-Chieh Liao, Tianying Lan, Charlotte Lindqvist, Frank A Scannapieco
BACKGROUND: Successful commensal bacteria have evolved to maintain colonization in challenging environments. The oral viridans streptococci are pioneer colonizers of dental plaque biofilm. Some of these bacteria have adapted to life in the oral cavity by binding salivary α-amylase, which hydrolyzes dietary starch, thus providing a source of nutrition. Oral streptococcal species bind α-amylase by expressing a variety of amylase-binding proteins (ABPs). Here we determine the genotypic basis of amylase binding where proteins of diverse size and function share a common phenotype...
April 20, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28427328/erratum-to-the-nasopharyngeal-microbiota-of-beef-cattle-before-and-after-transport-to-a-feedlot
#4
Devin B Holman, Edouard Timsit, Samat Amat, D Wade Abbott, Andre G Buret, Trevor W Alexander
No abstract text is available yet for this article.
April 20, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28415978/bacterial-communities-and-metabolic-activity-of-faecal-cultures-from-equol-producer-and-non-producer-menopausal-women-under-treatment-with-soy-isoflavones
#5
Lucía Guadamuro, Anja B Dohrmann, Christoph C Tebbe, Baltasar Mayo, Susana Delgado
BACKGROUND: Isoflavones are polyphenols with estrogenic activity found mainly in soy and soy-derived products that need to be metabolised in the intestine by the gut bacteria to be fully active. There is little knowledge about isoflavone bioconversion and equol production in the human intestine. In this work, we developed an in vitro anaerobic culture model based on faecal slurries to assess the impact of isoflavone supplementation on the overall intestinal bacterial composition changes and associated metabolic transformations...
April 17, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28407745/the-expression-of-fibronectin-is-significantly-suppressed-in-macrophages-to-exert-a-protective-effect-against-staphylococcus-aureus-infection
#6
Hong-Yi Chen, Mei-Hui Lin, Chien-Cheng Chen, Jwu-Ching Shu
BACKGROUND: Fibronectin (Fn) plays a major role in the attachment of Staphylococcus aureus to host cells by bridging staphylococcal fibronectin-binding proteins (FnBPs) and cell-surface integrins. A previous study demonstrated that the phagocytosis of S. aureus by macrophages is enhanced in the presence of exogenous Fn. We recently found that FnBPs overexpression also enhances phagocytic activity. The effect of S. aureus infection on the expression of macrophage Fn was investigated. RESULT: The level of Fn secreted by monocytes (THP-1), macrophages, human lung adenocarcinoma (A549) cells, and hepatocellular carcinoma (HepG2) cells in response to S...
April 13, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28403826/erratum-to-multidrug-resistance-protein-mdtm-adds-to-the-repertoire-of-antiporters-involved-in-alkaline-ph-homeostasis-in-escherichia-coli
#7
Scarlett R Holdsworth, Christopher J Law
No abstract text is available yet for this article.
April 12, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28399822/dominant-bacterial-phyla-in-caves-and-their-predicted-functional-roles-in-c-and-n-cycle
#8
Surajit De Mandal, Raghunath Chatterjee, Nachimuthu Senthil Kumar
BACKGROUND: Bacteria present in cave often survive by modifying their metabolic pathway or other mechanism. Understanding these adopted bacteria and their survival strategy inside the cave is an important aspect of microbial ecology. Present study focuses on the bacterial community and geochemistry in five caves of Mizoram, Northeast India. The objective of this study was to explore the taxonomic composition and presumed functional diversity of cave sediment metagenomes using paired end Illumina sequencing using V3 region of 16S rRNA gene and bioinformatics pipeline...
April 11, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28381253/localization-of-staphylococcus-aureus-in-tissue-from-the-nasal-vestibule-in-healthy-carriers
#9
Anne-Merethe Hanssen, Bert Kindlund, Niels Christian Stenklev, Anne-Sofie Furberg, Silje Fismen, Renate Slind Olsen, Mona Johannessen, Johanna Ulrica Ericson Sollid
BACKGROUND: Colonization of the body is an important step in Staphylococcus aureus infection. S. aureus colonizes skin and mucous membranes in humans and several animal species. One important ecological niche of S. aureus is the anterior nares. More than 60% of the S. aureus in the nose are found in vestibulum nasi. Our aim was to describe the localization of S. aureus in nasal tissue from healthy carriers. METHODS: Punch skin biopsies were taken from vestibulum nasi from healthy volunteers (S...
April 5, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28381209/supplemental-invasion-of-salmonella-from-the-perspective-of-salmonella-enterica-serovars-kentucky-and-typhimurium
#10
Kevin Howe, Sanaz Salehi, R Hartford Bailey, John P Brooks, Robert Wills, Mark L Lawrence, Attila Karsi
BACKGROUND: Critical to the development of Salmonellosis in humans is the interaction of the bacterium with the epithelial lining of the gastrointestinal tract. Traditional scientific reasoning held type III secretion system (T3SS) as the virulence factor responsible for bacterial invasion. In this study, field-isolated Salmonella enterica serovar Kentucky and a known human pathogen Salmonella enterica serovar Typhimurium were mutated and evaluated for the invasion of human colorectal adenocarcinoma epithelial cells...
April 5, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28376744/effects-of-pseudomonas-aeruginosa-and-streptococcus-mitis-mixed-infection-on-tlr4-mediated-immune-response-in-acute-pneumonia-mouse-model
#11
Chao Song, Hongdong Li, Yunhui Zhang, Jialin Yu
BACKGROUND: Our previous research on the diversity of microbiota in the endotracheal tubes (ETTs) of neonates in the neonatal intensive care unit found that Pseudomonas aeruginosa (P. aeruginosa) and Streptococcus mitis (S. mitis) were the dominant bacteria on the ETT surface and the existence of S. mitis could promote biofilm formation and pathogenicity of P. aeruginosa. Toll-like receptor 4 (TLR4), which has been widely detected on the surface of airway epithelial cells, is the important component of the innate immune system...
April 4, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28376742/polynucleotide-phosphorylase-is-implicated-in-homologous-recombination-and-dna-repair-in-escherichia-coli
#12
Thomas Carzaniga, Giulia Sbarufatti, Federica Briani, Gianni Dehò
BACKGROUND: Polynucleotide phosphorylase (PNPase, encoded by pnp) is generally thought of as an enzyme dedicated to RNA metabolism. The pleiotropic effects of PNPase deficiency is imputed to altered processing and turnover of mRNAs and small RNAs, which in turn leads to aberrant gene expression. However, it has long since been known that this enzyme may also catalyze template-independent polymerization of dNDPs into ssDNA and the reverse phosphorolytic reaction. Recently, PNPase has been implicated in DNA recombination, repair, mutagenesis and resistance to genotoxic agents in diverse bacterial species, raising the possibility that PNPase may directly, rather than through control of gene expression, participate in these processes...
April 4, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28376730/application-of-hierarchical-oligonucleotide-primer-extension-hope-to-assess-relative-abundances-of-ammonia-and-nitrite-oxidizing-bacteria
#13
Giantommaso Scarascia, Hong Cheng, Moustapha Harb, Pei-Ying Hong
BACKGROUND: Establishing an optimal proportion of nitrifying microbial populations, including ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), complete nitrite oxidizers (comammox) and ammonia-oxidizing archaea (AOA), is important for ensuring the efficiency of nitrification in water treatment systems. Hierarchical oligonucleotide primer extension (HOPE), previously developed to rapidly quantify relative abundances of specific microbial groups of interest, was applied in this study to track the abundances of the important nitrifying bacterial populations...
April 4, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28376723/discrimination-of-three-genetically-close-aspergillus-species-by-using-high-resolution-melting-analysis-applied-to-indoor-air-as-case-study
#14
Xavier Libert, Ann Packeu, Fabrice Bureau, Nancy H Roosens, Sigrid C J De Keersmaecker
BACKGROUND: Indoor air pollution caused by fungal contamination is suspected to have a public health impact. Monitoring of the composition of the indoor airborne fungal contaminants is therefore important. To avoid problems linked to culture-dependent protocols, molecular methods are increasingly being proposed as an alternative. Among these molecular methods, the polymerase chain reaction (PCR) and the real-time PCR are the most frequently used tools for indoor fungal detection. However, even if these tools have demonstrated their appropriate performance, some of them are not able to discriminate between species which are genetically close...
April 4, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28376722/evolutionary-gradient-of-predicted-nuclear-localization-signals-nls-bearing-proteins-in-genomes-of-family-planctomycetaceae
#15
Min Guo, Ruifu Yang, Chen Huang, Qiwen Liao, Guangyi Fan, Chenghang Sun, Simon Ming-Yuen Lee
BACKGROUND: The nuclear envelope is considered a key classification marker that distinguishes prokaryotes from eukaryotes. However, this marker does not apply to the family Planctomycetaceae, which has intracellular spaces divided by lipidic intracytoplasmic membranes (ICMs). Nuclear localization signal (NLS), a short stretch of amino acid sequence, destines to transport proteins from cytoplasm into nucleus, and is also associated with the development of nuclear envelope. We attempted to investigate the NLS motifs in Planctomycetaceae genomes to demonstrate the potential molecular transition in the development of intracellular membrane system...
April 4, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28376716/in-vitro-transfer-of-methicillin-resistance-determinants-meca-from-methicillin-resistant-staphylococcus-aureus-mrsa-to-methicillin-susceptible-staphylococcus-aureus-mssa
#16
Asinamai Athliamai Bitrus, Zakaria Zunita, Siti Khairani Bejo, Sarah Othman, Nur Adilah Ahmad Nadzir
BACKGROUND: Staphylococcus aureus more than any other human pathogen is a better model for the study of the adaptive evolution of bacterial resistance to antibiotics, as it has demonstrated a remarkable ability in its response to new antibiotics. This study was designed to investigate the in vitro transfer of mecA gene from methicillin resistant S. aureus to methicillin susceptible S. aureus. RESULT: The recipient transconjugants were resistant to erythromycin, cefpodoxime and were mecA positive...
April 4, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28376715/initial-nitrogen-enrichment-conditions-determines-variations-in-nitrogen-substrate-utilization-by-heterotrophic-bacterial-isolates
#17
Suchismita Ghosh, Paul A Ayayee, Oscar J Valverde-Barrantes, Christopher B Blackwood, Todd V Royer, Laura G Leff
BACKGROUND: The nitrogen (N) cycle consists of complex microbe-mediated transformations driven by a variety of factors, including diversity and concentrations of N compounds. In this study, we examined taxonomic diversity and N substrate utilization by heterotrophic bacteria isolated from streams under complex and simple N-enrichment conditions. RESULTS: Diversity estimates differed among isolates from the enrichments, but no significant composition were detected...
April 4, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28376713/prevalence-and-antimicrobial-resistance-of-campylobacter-jejuni-and-campylobacter-coli-isolated-from-children-and-environmental-sources-in-urban-and-suburban-areas
#18
Bernadeta Szczepanska, Małgorzata Andrzejewska, Dorota Spica, Jacek J Klawe
BACKGROUND: Campylobacteriosis is a dominant bacterial cause of foodborne infection and is considered the main public health problem in Europe and many other countries worldwide. In the study lasting from 2011 to 2013 we compared the prevalence and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from children, domestic animals, poultry meat and surface water in Northern Poland. RESULTS: During a 3-years study 1973 samples were analysed...
April 4, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28359329/comparison-of-stool-versus-rectal-swab-samples-and-storage-conditions-on-bacterial-community-profiles
#19
Christine M Bassis, Nicholas M Moore, Karen Lolans, Anna M Seekatz, Robert A Weinstein, Vincent B Young, Mary K Hayden
BACKGROUND: Sample collection for gut microbiota analysis from in-patients can be challenging. Collection method and storage conditions are potential sources of variability. In this study, we compared the bacterial microbiota from stool stored under different conditions, as well as stool and swab samples, to assess differences due to sample storage conditions and collection method. METHODS: Using bacterial 16S rRNA gene sequence analysis, we compared the microbiota profiles of stool samples stored and collected under various conditions...
March 31, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28359254/development-of-a-genus-specific-next-generation-sequencing-approach-for-sensitive-and-quantitative-determination-of-the-legionella-microbiome-in-freshwater-systems
#20
Rui P A Pereira, Jörg Peplies, Ingrid Brettar, Manfred G Höfle
BACKGROUND: Next Generation Sequencing (NGS) has revolutionized the analysis of natural and man-made microbial communities by using universal primers for bacteria in a PCR based approach targeting the 16S rRNA gene. In our study we narrowed primer specificity to a single, monophyletic genus because for many questions in microbiology only a specific part of the whole microbiome is of interest. We have chosen the genus Legionella, comprising more than 20 pathogenic species, due to its high relevance for water-based respiratory infections...
March 31, 2017: BMC Microbiology
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