journal
MENU ▼
Read by QxMD icon Read
search

Journal of Biological Chemistry

journal
https://www.readbyqxmd.com/read/29678884/physiological-temperatures-reduce-dimerization-of-dengue-and-zika-virus-recombinant-envelope-proteins
#1
Stephan T Kudlacek, Lakshmanane Premkumar, Stefan W Metz, Ashutosh Tripathy, Andrey A Bobkov, Alexander Matthew Payne, Stephen Graham, James A Brackbill, Michael J Miley, Aravinda M de Silva, Brian Kuhlman
The spread of dengue (DENV) and Zika virus (ZIKV) is a major public health concern. The primary target of antibodies (Abs) that neutralize DENV and ZIKV is the envelope (E) glycoprotein, and there is interest in using soluble recombinant E (sRecE) proteins as subunit vaccines. However, the most potent neutralizing antibodies against DENV and ZIKV recognize epitopes on the virion surface that span two or more E proteins. Therefore, to create effective DENV and ZIKV vaccines, presentation of these quaternary epitopes may be necessary...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29678883/ribosome-maturation-by-the-endoribonuclease-ybey-stabilises-a-type-iii-secretion-system-transcript-required-for-virulence-of-enterohemorrhagic-escherichia-coli
#2
Sean P McAteer, Brandon M Sy, Julia L Wong, David Tollervey, David L Gally, Jai J Tree
Enterohemorrhagic Escherichia coli (EHEC) is a significant human pathogen that colonizes humans and its reservoir host, cattle. Colonization requires the expression of a type III secretion (T3S) system that injects a cocktail of effector proteins into host cells, to promote bacterial attachment and disease progression. The T3S system is tightly regulated by a complex network of transcriptional and post-transcriptional regulators. Using transposon mutagenesis, here we identified the ybeZYX-int operon as being required for normal T3S levels...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29678882/mesenchyme-homeobox-1-mediates-transforming-growth-factor-%C3%AE-tgf-%C3%AE-induced-smooth-muscle-cell-differentiation-from-mouse-mesenchymal-progenitors
#3
Kun Dong, Xia Guo, Weiping Chen, Amanda C Hsu, Qiang Shao, Jian-Fu Chen, Shi-You Chen
Differentiation of smooth muscle cells (SMCs) is critical for proper vasculogenesis and angiogenesis. However, the molecular mechanisms controlling SMC differentiation are not completely understood. During embryogenesis, the transcription factor mesenchyme homeobox 1 (Meox1) is expressed in the early developing somite, which is one of the origins of SMCs. In the present study, we identified Meox1 as a positive regulator of SMC differentiation. We found that transforming growth factor-β (TGF-β) induces Meox1 expression in the initial phase of SMC differentiation of pluripotent murine C3H10T1/2 cells...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29678881/regulation-of-c-c-chemokine-receptor-5-ccr5-stability-by-lys-197-and-by-transmembrane-protein-aptamers-that-target-it-for-lysosomal-degradation
#4
Lisa M Petti, Sara A Marlatt, Yong Luo, Elizabeth H Scheideman, Ashish Shelar, Daniel DiMaio
C-C motif chemokine receptor 5 (CCR5) is a cell surface-associated, immune-regulatory G protein-coupled receptor (GCPR) with seven transmembrane helices. We previously reported the isolation and initial characterization of short artificial transmembrane protein aptamers, named "traptamers," that specifically down-regulate CCR5 expression and inhibit infection of human T cells by HIV strains that use CCR5 as a co-receptor. Here, we investigated the mechanism of traptamer-mediated CCR5 down-regulation and show that most of the traptamers (designated class 1 traptamers) form a stable complex with CCR5 and target it for lysosome-mediated degradation...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29678880/structural-and-thermodynamic-insights-into-%C3%AE-1-2-glucooligosaccharide-capture-by-a-solute-binding-protein-in-listeria-innocua
#5
Koichi Abe, Naoki Sunagawa, Tohru Terada, Yuta Takahashi, Takatoshi Arakawa, Kiyohiko Igarashi, Masahiro Samejima, Hiroyuki Nakai, Hayao Taguchi, Masahiro Nakajima, Shinya Fushinobu
β-1,2-Glucans are bacterial carbohydrates that exist in cyclic or linear forms and play an important role in infections and symbioses involving Gram-negative bacteria. Although several β-1,2-glucan-associated enzymes have been characterized, little is known about how β-1,2-glucan and its shorter oligosaccharides (Sopn s) are captured and imported into the bacterial cell. Here, we report the biochemical and structural characteristics of Sopn -binding protein (SO-BP, Lin1841) associated with the ABC transporter from the Gram-positive bacterium Listeria innocua Calorimetric analysis revealed that SO-BP specifically binds to Sopn s with degree of polymerization of 3 or more, with Kd  values in the micromolar range...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29678879/potassium-is-a-trigger-for-conformational-change-in-the-fusion-spike-of-an-enveloped-rna-virus
#6
Emma K Punch, Samantha Hover, Henry Tw Blest, Jack Fuller, Roger Hewson, Juan Fontana, Jamel Mankouri, John N Barr
Many enveloped viruses enter cells through the endocytic network, from which they must subsequently escape through fusion of viral and endosomal membranes. This membrane fusion is mediated by virus-encoded spikes that respond to the changing endosomal environment, which triggers conformational change in the spike that initiate the fusion process. Several fusion triggers have been identified and include pH, membrane composition and endosome-resident proteins and these cues dictate when and where viral fusion occurs...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29678878/a-bispecific-antibody-that-targets-il-6-receptor-and-il-17a-for-the-potential-therapy-of-patients-with-autoimmune-and-inflammatory-diseases
#7
Michael Lyman, Vincent Lieuw, Robyn Richardson, Anjuli Timmer, Christine Stewart, Steve Granger, Richard Woods, Michela Silacci, Dragan Grabulovski, Roland Newman
Despite the success of current biological therapeutics for rheumatoid arthritis, these therapies, targeting individual cytokines or pathways, produce beneficial responses in only about half of patients. Therefore, better therapeutics are needed. IL-6 and IL-17A are pro-inflammatory cytokines in many autoimmune and inflammatory diseases, and several therapeutics have been developed to specifically inhibit them. However, targeting both of these cytokines with a bispecific therapeutic agent could account for their non-overlapping pro-inflammatory functions and for the fact that IL-6 and IL-17A act in a positive feedback loop...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29674345/quantitative-interaction-analysis-permits-molecular-insights-into-functional-nox4-nadph-oxidase-heterodimer-assembly
#8
Sharon O'Neill, Magalie Mathis, Lidija Kovacic, Suisheng Zhang, Juergen Reinhardt, Dimitri Scholz, Ulrich Schopfer, Rochdi Bouhelal, Ulla G Knaus
Protein-protein interactions critically regulate many biological systems, but quantifying functional assembly of multi-pass membrane complexes in their native context is still challenging. Here, we combined modeling-assisted protein modification and information from human disease variants with a minimal-size fusion tag, split-luciferase-based approach to probe assembly of the NADPH oxidase 4 (NOX4)-p22phox enzyme, an integral membrane complex with unresolved structure, which is required for electron transfer and generation of reactive oxygen species (ROS)...
April 19, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29674319/long-repeating-ttaggg-n-single-stranded-dna-self-condenses-into-compact-beaded-filaments-stabilized-by-g-quadruplex-formation
#9
Anirban Kar, Nathan Jones, N Özlem Arat, Richard Fishel, Jack Griffith
Conformations adopted by long stretches of single stranded DNA (ssDNA) are of central interest in understanding the architecture of replication forks, R loops, and other structures generated during DNA metabolism in vivo. This is particularly so if the ssDNA consists of short nucleotide repeats. Such studies have been hampered by the lack of defined substrates greater than ~150 nt, and the absence of high-resolution biophysical approaches. Here we describe the generation of very long ssDNA consisting of the mammalian telomeric repeat (5'-TTAGGG-3')n as well as the interrogation of its structure by electron microscopy (EM) and single molecule magnetic tweezers (smMT)...
April 19, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29674318/nicotiana-benthamiana-%C3%AE-galactosidase-a1-1-can-functionally-complement-human-%C3%AE-galactosidase-a-deficiency-associated-with-fabry-disease
#10
Kassiani Kytidou, Jules Beekwilder, Marta Artola, Eline van Meel, Ruud H P Wilbers, Geri F Moolenaar, Nora Goosen, Maria J Ferraz, Rebecca Katzy, Patrick Voskamp, Bogdan I Florea, Cornelis H Hokke, Herman S Overkleeft, Arjen Schots, Dirk Bosch, Navraj Pannu, Johannes M F G Aerts
α-Galactosidases (EC 3.2.1.22) are retaining glycosidases that cleave terminal α-linked galactose residues from glycoconjugate substrates. α-Galactosidases take part in the turnover of cell wall-associated galactomannans in plants and in the lysosomal degradation of glycosphingolipids in animals. Deficiency of human α-galactosidase A (α-Gal A) causes Fabry disease (FD), a heritable, X-linked lysosomal storage disorder, characterized by accumulation of globotriaosylceramide (Gb3) and globotriaosylsphingosine (lysoGb3)...
April 19, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29674317/the-atypical-mitogen-activated-protein-kinase-erk3-is-essential-for-establishment-of-epithelial-architecture
#11
Chika Takahashi, Koichi Miyatake, Morioh Kusakabe, Eisuke Nishida
Epithelia contribute to physical barriers that protect internal tissues from the external environment and also support organ structure. Accordingly, establishment and maintenance of epithelial architecture are essential for both embryonic development and adult physiology. Here, using gene knockout and -down techniques along with gene profiling, we show that extracellular signal-regulated kinase 3 (ERK3), a poorly characterized atypical mitogen-activated protein kinase (MAPK), regulates the epithelial architecture in vertebrates...
April 19, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29674316/activation-of-renal-clc-k-chloride-channels-depends-on-an-intact-n-terminus-of-their-accessory-subunit-barttin
#12
Daniel Wojciechowski, Stefan Thiemann, Christina Schaal, Alina Rahtz, Jeanne de la Roche, Birgit Begemann, Toni Becher, Martin Fischer
ClC-K channels belong to the CLC family of chloride channels and chloride/proton antiporters. They contribute to sodium chloride reabsorption in Henle's loop of the kidney and to potassium secretion into the endolymph by the stria vascularis of the inner ear. Their accessory subunit barttin stabilizes the ClC-K/barttin complex, promotes its insertion into the surface membrane, and turns the pore-forming subunits into a conductive state. Barttin mutations cause Bartter syndrome type IV, a salt wasting nephropathy with sensorineural deafness...
April 19, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29669813/monophosphorylation-of-cardiac-troponin-i-at-ser23-24-is-sufficient-to-regulate-cardiac-myofibrillar-ca-2-sensitivity-and-calpain-induced-proteolysis
#13
Abel Martin-Garrido, Brandon J Biesiadecki, Hussam E Salhi, Yasin Shaifta, Cristobal G Dos Remedios, Serife Ayuz-Guner, Wenxuan Cai, Ying Ge, Metin Avkiran, Jonathan C Kentish
The acceleration of myocardial relaxation produced by β-adrenoceptor stimulation is mediated in part by protein kinase A (PKA)-mediated phosphorylation of cardiac troponin-I (cTnI), which decreases myofibrillar Ca2+ sensitivity. Previous evidence suggests that phosphorylation of both Ser23 and Ser24 in cTnI is required for this Ca2+ desensitization. PKA-mediated phosphorylation also partially protects cTnI from proteolysis by calpain. Here we report that protein kinase D (PKD) phosphorylates only one serine of cTnI Ser23/24...
April 18, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29669812/tankyrase-modulates-insulin-sensitivity-in-skeletal-muscle-cells-by-regulating-the-stability-of-glut4-vesicle-proteins
#14
Zhiduan Su, Vinita Deshpande, David E James, Jacqueline Stöckli
Tankyrase1 and 2, members of the poly(ADP-ribose) polymerase family, have previously been shown to play a role in insulin-mediated glucose uptake in adipocytes. However, their precise mechanism of action, and their role in insulin action in other cell types, such as myocytes, remains elusive. Treatment of differentiated L6 myotubes with the small molecule tankyrase inhibitor XAV939 resulted in insulin resistance as determined by impaired insulin-stimulated glucose uptake. Proteomic analysis of XAV939-treated myotubes identified down-regulation of several glucose transporter GLUT4 storage vesicle (GSV) proteins including RAB10, VAMP8, SORT1 and GLUT4...
April 18, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29669811/linker-engineering-in-anti-tag-72-antibody-fragments-optimizes-biophysical-properties-serum-half-life-and-high-specificity-tumor-imaging
#15
Nicholas E Long, Brandon J Sullivan, Haiming Ding, Stephanie R Doll, Michael A Ryan, Charles L Hitchcock, Edward W Martin, Krishan Kumar, Michael F Tweedle, Thomas J Magliery
Antibody (Ab) fragments have great clinical potential as cancer therapeutics and diagnostics. Their small size allows for fast clearance from blood, low immunoreactivity, better tumor penetration, and simpler engineering and production. The smallest fragment derived from a full-length IgG that retains binding to its antigen, the single-chain variable fragment (scFV), is engineered by fusing the variable light and variable heavy domains with a peptide linker. Along with switching the domain orientation, altering the length and amino acid sequence of the linker can significantly affect scFV binding, stability, quaternary structure, and other biophysical properties...
April 18, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29669810/structural-insights-into-the-mechanism-of-action-of-a-biparatopic-anti-her2-antibody
#16
Vaheh Oganesyan, Li Peng, Jared S Bee, John Li, Samuel R Perry, Frank Comer, Linda Xu, Kimberly Cook, Kannaki Senthil, Lori Clarke, Kim Rosenthal, Chanshou Gao, Melissa Damschroder, Herren Wu, William F Dall'Acqua
Pathways of human EGF receptors are activated upon ligand dependent or independent homo or heterodimerization and their subsequent transphosphorylation. Overexpression of these receptors positively correlates with transphosphorylation rates and increased tumor growth rates. MEDI4276, an anti human epidermal growth factor receptor 2 (HER2) biparatopic antibody drug conjugate, has two paratopes within each of antibody arms. One, 39S, is aiming at the HER2 site involved in receptor dimerization and the second, single chain fragment (scFv) mimicking trastuzumab...
April 18, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29669809/two-distinct-sites-of-client-protein-interaction-with-the-chaperone-cpsrp43
#17
Camille McAvoy, Alex Siegel, Samantha Piszkiewicz, Emily Miaou, Mansen Yu, Thang Nguyen, Annie Moradian, Michael J Sweredoski, Sonja Hess, Shu-Ou Shan
Integral membrane proteins are prone to aggregation and misfolding in aqueous environments and therefore require binding by molecular chaperones during their biogenesis. Chloroplast signal recognition particle 43 (cpSRP43) is an ATP-independent chaperone required for the biogenesis of the most abundant class of membrane proteins, the light-harvesting chlorophyll a/b-binding proteins (LHCPs). Previous work has shown that cpSRP43 specifically recognizes an L18 loop sequence conserved among LHCP paralogs. However, how cpSRP43 protects the transmembrane domains (TMDs) of LHCP from aggregation was unclear...
April 18, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29669808/lactoferrin-is-a-natural-inhibitor-of-plasminogen-activation
#18
Alexander Zwirzitz, Michael Reiter, Rostislav Skrabana, Anna Ohradanova-Repic, Otto Majdic, Marianna Gutekova, Ondrej Cehlar, Eva Petrovčíková, Eva Kutejova, Gerold Stanek, Hannes Stockinger, Vladimir Leksa
The plasminogen system is essential for dissolution of fibrin clots, and in addition, it is involved in a wide variety of other physiological processes, including proteolytic activation of growth factors, cell migration, and removal of protein aggregates. On the other hand, uncontrolled plasminogen activation contributes to many pathological processes, e.g. tumor cells' invasion in cancer progression. Moreover, some virulent bacterial species, e.g. Streptococci or Borrelia, bind human plasminogen and hijack the host's plasminogen system to penetrate tissue barriers...
April 18, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29669807/pqsl-uses-reduced-flavin-to-produce-2-hydroxylaminobenzoylacetate-a-preferred-pqsbc-substrate-in-alkyl-quinolone-biosynthesis-in-pseudomonas-aeruginosa
#19
Steffen Lorenz Drees, Simon Ernst, Benny Danilo Belviso, Nina Jagmann, Ulrich Hennecke, Susanne Fetzner
Alkyl hydroxyquinoline N -oxides (AQNOs) are antibiotic compounds produced by the opportunistic bacterial pathogen Pseudomonas aeruginosa They are products of the alkyl quinolone (AQ) biosynthetic pathway, which also generates the quorum sensing molecules 2-heptyl-4(1 H )-quinolone (HHQ) and 2-heptyl-3-hydroxy-4(1 H )-quinolone (PQS). Although the enzymatic synthesis of HHQ and PQS had been elucidated, the route by which AQNOs are synthesized remained elusive. Here, we report on PqsL, the key enzyme for AQNO production, which structurally resembles class A flavoprotein monooxygenases such as p -hydroxybenzoate 3-hydroxylase (pHBH) and 3-hydroxybenzoate 6-hydroxylase...
April 18, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29666193/lmo1656-is-a-secreted-virulence-factor-of-listeria-monocytogenes-that-interacts-with-the-sortin-nexin-6-bar-complex
#20
Daryl Jason David, Alessandro Pagliuso, Lilliana Radoshevich, Marie-Anne Nahori, Pascale Cossart
Listeria monocytogenes (Lm) is a facultative intracellular bacterial pathogen, the causative agent of listeriosis, a rare but fatal disease.  During infection, Lm can traverse several physiological barriers; it can cross the intestine and placenta, and in immunocompromised individuals, the blood-brain barrier. With the recent plethora of sequenced genomes available for Lm, it is clear that the complete repertoire of genes used by Lm to interact with its host remains to be fully explored.  Recently, we focused on secreted Lm proteins, since they are likely to interact with host cell components...
April 17, 2018: Journal of Biological Chemistry
journal
journal
20327
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"