journal
https://read.qxmd.com/read/38391233/archaeal-actins-and-the-origin-of-a-multi-functional-cytoskeleton
#21
REVIEW
Arthur Charles-Orszag, Natalie A Petek-Seoane, R Dyche Mullins
Actin and actin-like proteins form filamentous polymers that carry out important cellular functions in all domains of life. In this review, we sketch a map of the function and regulation of actin-like proteins across bacteria, archaea, and eukarya, marking some of the terra incognita that remain in this landscape. We focus particular attention on archaea because mapping the structure and function of cytoskeletal systems across this domain promises to help us understand the evolutionary relationship between the (mostly) mono-functional actin-like filaments found in bacteria and the multi-functional actin cytoskeletons that characterize eukaryotic cells...
February 23, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38391161/biological-characteristics-of-mechanosensitive-channels-mscs-and-mscl-in-actinobacillus-pleuropneumoniae
#22
JOURNAL ARTICLE
Jiajia Wan, Lu Dai, Huasong Xiao, Wendie Zhang, Rui Zhang, Tingting Xie, Yizhen Jia, Xuejun Gao, Jing Huang, Feng Liu
Actinobacillus pleuropneumoniae is an important respiratory pathogen that can cause porcine contagious pleuropneumonia (PCP), resulting in significant economic losses in swine industry. Microorganisms are subjected to drastic changes in environmental osmolarity. In order to alleviate the drastic rise or fall of osmolarity, cells activate mechanosensitive channels MscL and MscS through tension changes. MscL not only regulates osmotic pressure but also has been reported to secrete protein and uptake aminoglycoside antibiotic...
February 23, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38376203/identification-of-clostridioides-difficile-mutants-with-increased-daptomycin-resistance
#23
JOURNAL ARTICLE
Brianne R Zbylicki, Claire E Murphy, Jennifer A Petsche, Ute Müh, Horia A Dobrila, Theresa D Ho, Mikaela N Daum, Anthony G Pannullo, David S Weiss, Craig D Ellermeier
Daptomycin is a cyclic lipopeptide antibiotic used to treat infections caused by some Gram-positive bacteria. Daptomycin disrupts synthesis of the peptidoglycan (PG) cell wall by inserting into the cytoplasmic membrane and binding multiple forms of the undecaprenyl carrier lipid required for PG synthesis. Membrane insertion requires phosphatidylglycerol, so studies of daptomycin can provide insight into assembly and maintenance of the cytoplasmic membrane. Here, we studied the effects of daptomycin on Clostridioides difficile , the leading cause of healthcare-associated diarrhea...
February 20, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38358279/abundant-urinary-amino-acids-activate-glutamine-synthetase-encoding-glna-by-two-different-mechanisms-in-escherichia-coli
#24
JOURNAL ARTICLE
Karthik Urs, Philippe E Zimmern, Larry Reitzer
Growth of uropathogenic Escherichia coli in the bladder induces transcription of glnA which codes for the ammonia-assimilating glutamine synthetase (GS) despite the normally suppressive high ammonia concentration. We previously showed that the major urinary component, urea, induces transcription from the Crp-dependent glnAp1 promoter, but the urea-induced transcript is not translated. Our purpose here was to determine whether the most abundant urinary amino acids, which are known to inhibit GS activity in vitro , also affect glnA transcription in vivo ...
February 15, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38358278/bacterial-nucleoid-is-a-riddle-wrapped-in-a-mystery-inside-an-enigma
#25
REVIEW
Andrei Kuzminov
Bacterial chromosome, the nucleoid, is traditionally modeled as a rosette of DNA mega-loops, organized around proteinaceous central scaffold by nucleoid-associated proteins (NAPs), and mixed with the cytoplasm by transcription and translation. Electron microscopy of fixed cells confirms dispersal of the cloud-like nucleoid within the ribosome-filled cytoplasm. Here, I discuss evidence that the nucleoid in live cells forms DNA phase separate from riboprotein phase, the "riboid." I argue that the nucleoid-riboid interphase, where DNA interacts with NAPs, transcribing RNA polymerases, nascent transcripts, and ssRNA chaperones, forms the transcription zone...
February 15, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38353531/-streptomyces-extracellular-vesicles-are-a-broad-and-permissive-antimicrobial-packaging-and-delivery-system
#26
JOURNAL ARTICLE
Kirsten J Meyer, Justin R Nodwell
Streptomyces are the primary source of bioactive specialized metabolites used in research and medicine, including many antimicrobials. These are presumed to be secreted and function as freely soluble compounds. However, increasing evidence suggests that extracellular vesicles are an alternative secretion system. We assessed environmental and lab-adapted Streptomyces (sporulating filamentous actinomycetes) and found frequent production of antimicrobial vesicles. The molecular cargo included actinomycins, anthracyclines, candicidin, and actinorhodin, reflecting both diverse chemical properties and diverse antibacterial and antifungal activity...
February 14, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38353530/the-ssgb-gene-is-required-for-the-early-stages-of-sporangium-formation-in-actinoplanes-missouriensis
#27
JOURNAL ARTICLE
Takuya Akutsu, Takeaki Tezuka, Manato Maruko, Aiko Hirata, Yasuo Ohnishi
In Streptomyces , multiple paralogs of SsgA-like proteins (SALPs) are involved in spore formation from aerial hyphae. However, the functions of SALPs have not yet been elucidated in other actinobacterial genera. Here, we report the primary function of an SsgB ortholog (AmSsgB) in Actinoplanes missouriensis , which develops terminal sporangia on the substrate mycelia via short sporangiophores. Importantly, AmSsgB is the sole SALP in A. missouriensis . The transcription of AmssgB was upregulated during sporangium formation, consistent with our previous findings that AmssgB is a member of the AmBldD regulon...
February 14, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38353529/an-adapted-method-for-cas9-mediated-editing-reveals-the-species-specific-role-of-%C3%AE-glucoside-utilization-driving-competition-between-klebsiella-species
#28
JOURNAL ARTICLE
Éva D H Almási, Nele Knischewski, Lisa Osbelt, Uthayakumar Muthukumarasamy, Youssef El Mouali, Elena Vialetto, Chase L Beisel, Till Strowig
Cas9-based gene editing tools have revolutionized genetics, enabling the fast and precise manipulation of diverse bacterial species. However, widely applicable genetic tools for non-model gut bacteria are unavailable. Here, we present a two-plasmid Cas9-based system designed for gene deletion and knock-in complementation in three members of the Klebsiella oxytoca species complex (KoSC), which we applied to study the genetic factors underlying the role of these bacteria in competition against Klebsiella pneumoniae ...
February 14, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38334326/vaginal-community-state-types-csts-alter-environmental-cues-and-production-of-the-staphylococcus-aureus-toxic-shock-syndrome-toxin-1-tsst-1
#29
JOURNAL ARTICLE
Carla S Maduta, John K McCormick, Karine Dufresne
Menstrual toxic shock syndrome (mTSS) is a rare but life-threatening disease associated with the use of high-absorbency tampons. The production of the Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1) superantigen is involved in nearly all cases of mTSS and is tightly controlled by regulators responding to the environment. In the prototypic mTSS strain S. aureus MN8, the major repressor of TSST-1 is the carbon catabolite protein A (CcpA), which responds to glucose concentrations in the vaginal tract...
February 9, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38323910/the-bacillus-subtilis-cell-envelope-stress-inducible-ytpab-operon-modulates-membrane-properties-and-contributes-to-bacitracin-resistance
#30
JOURNAL ARTICLE
Jessica R Willdigg, Yesha Patel, Briana E Arquilevich, Chitra Subramanian, Matthew W Frank, Charles O Rock, John D Helmann
Antibiotics that inhibit peptidoglycan synthesis trigger the activation of both specific and general protective responses. σM responds to diverse antibiotics that inhibit cell wall synthesis. Here, we demonstrate that cell wall-inhibiting drugs, such as bacitracin and cefuroxime, induce the σM -dependent ytpAB operon. YtpA is a predicted hydrolase previously proposed to generate the putative lysophospholipid antibiotic bacilysocin (lysophosphatidylglycerol), and YtpB is the branchpoint enzyme for the synthesis of membrane-localized C35 terpenoids...
February 7, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38319218/a-dual-plasmid-crispr-cas9-based-method-for-rapid-and-efficient-genetic-disruption-in-mycobacterium-abscessus
#31
JOURNAL ARTICLE
Donavan Marcus Neo, Anne E Clatworthy, Deborah T Hung
Mycobacterium abscessus is increasingly recognized for causing infections that are notoriously difficult to treat, owing to its large arsenal of intrinsic antibiotic resistance mechanisms. Tools for the genetic manipulation of the pathogen are critical for enabling a better understanding of M. abscessus biology, pathogenesis, and antibiotic resistance mechanisms. However, existing methods are largely recombination-based, which are relatively inefficient. Meanwhile, CRISPR/Cas9 has revolutionized the field of genome editing including its recent adaptation for use in mycobacteria...
February 6, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38319100/multitier-regulation-of-the-e-coli-extreme-acid-stress-response-by-csra
#32
JOURNAL ARTICLE
Mark G Gorelik, Helen Yakhnin, Archana Pannuri, Alyssa C Walker, Christine Pourciau, Daniel Czyz, Tony Romeo, Paul Babitzke
CsrA is an RNA-binding protein that regulates processes critical for growth and survival, including central carbon metabolism, motility, biofilm formation, stress responses, and expression of virulence factors in pathogens. Transcriptomics studies in Escherichia coli suggested that CsrA repressed genes involved in surviving extremely acidic conditions. Here, we examine the effects of disrupting CsrA-dependent regulation on the expression of genes and circuitry for acid stress survival and demonstrate CsrA-mediated repression at multiple levels...
February 6, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38315013/pathogenic-rickettsia-spp-as-emerging-models-for-bacterial-biology
#33
REVIEW
Brandon Sit, Rebecca L Lamason
Our understanding of free-living bacterial models like Escherichia coli far outpaces that of obligate intracellular bacteria, which cannot be cultured axenically. All obligate intracellular bacteria are host-associated, and many cause serious human diseases. Their constant exposure to the distinct biochemical niche of the host has driven the evolution of numerous specialized bacteriological and genetic adaptations, as well as innovative molecular mechanisms of infection. Here, we review the history and use of pathogenic Rickettsia species, which cause an array of vector-borne vascular illnesses, as model systems to probe microbial biology...
February 5, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38305193/pyruvate-dependent-growth-of-methanosarcina-acetivorans
#34
JOURNAL ARTICLE
Marcus Richter, Christian Sattler, Christian Schöne, Michael Rother
Methanogenesis is a key step during anaerobic biomass degradation. Methanogenic archaea (methanogens) are the only organisms coupling methanogenic substrate conversion to energy conservation. The range of substrates utilized by methanogens is limited, with acetate and H2 +CO2 being the ecologically most relevant. The only single methanogenic energy substrate containing more carbon-carbon bonds than acetate is pyruvate. Only the aggregate-forming, freshwater methanogen Methanosarcina barkeri Fusaro was shown to grow on this compound...
February 2, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38299858/glutamate-dependent-arginine-biosynthesis-requires-the-inactivation-of-spovg-sara-and-ahrc-in-staphylococcus-aureus
#35
JOURNAL ARTICLE
Itidal Reslane, Luke D Handke, Gabrielle F Watson, Dhananjay Shinde, Jong-Sam Ahn, Jennifer L Endres, Fareha Razvi, Emily A Gilbert, Kenneth W Bayles, Vinai C Thomas, McKenzie K Lehman, Paul D Fey
Genome sequencing has demonstrated that Staphylococcus aureus encodes arginine biosynthetic genes argDCJBFGH synthesizing proteins that mediate arginine biosynthesis using glutamate as a substrate. Paradoxically, however, S. aureus does not grow in a defined, glutamate-replete medium lacking arginine and glucose (CDM-R). Studies from our laboratory have found that specific mutations are selected by S. aureus that facilitate growth in CDM-R. However, these selected mutants synthesize arginine utilizing proline as a substrate rather than glutamate...
February 1, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38299857/mechanism-of-2-fucosyllactose-degradation-by-human-associated-akkermansia
#36
JOURNAL ARTICLE
Loren Padilla, Ashwana D Fricker, Estefani Luna, Biswa Choudhury, Elizabeth R Hughes, Maria E Panzetta, Raphael H Valdivia, Gilberto E Flores
Among the first microorganisms to colonize the human gut of breastfed infants are bacteria capable of fermenting human milk oligosaccharides (HMOs). One of the most abundant HMOs, 2'-fucosyllactose (2'-FL), may specifically drive bacterial colonization of the intestine. Recently, differential growth has been observed across multiple species of Akkermansia on various HMOs including 2'-FL. In culture, we found growth of two species, A. muciniphila MucT and A. biwaensis CSUN-19,on HMOs corresponded to a decrease in the levels of 2'-FL and an increase in lactose, indicating that the first step in 2'-FL catabolism is the cleavage of fucose...
February 1, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38294238/-deoxy-to-be-or-desoxy-not-to-be-a-century-old-tale-in-the-history-of-dna-nomenclature
#37
JOURNAL ARTICLE
Gaurav Sharma, Jaimin Chodvadiya, Indranil Malik
This commentary discusses a comprehensive history of the first-ever use of pertinent words directly related to DNA, such as desoxyribose, deoxyribose, desoxyribonucleic acid, and deoxyribonucleic acid. With almost 100 years of the identification and nomenclature of desoxyribose sugar and desoxyribonucleic acid, the term "desoxy" continues to see limited use. We hope that whenever young researchers come across the sporadic occurrence of "desoxy" in any published text, they will not consider it a mistake.
January 31, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38289064/fitness-benefits-of-a-synonymous-substitution-in-an-ancient-ef-tu-gene-depend-on-the-genetic-background
#38
JOURNAL ARTICLE
Kaitlyn M McGrath, Steven J Russell, Evrim Fer, Eva Garmendia, Ali Hosgel, David A Baltrus, Betül Kaçar
Synonymous mutations are changes to DNA sequence, which occur within translated genes but which do not affect the protein sequence. Although often referred to as silent mutations, evidence suggests that synonymous mutations can affect gene expression, mRNA stability, and even translation efficiency. A collection of both experimental and bioinformatic data has shown that synonymous mutations can impact cell phenotype, yet less is known about the molecular mechanisms and potential of beneficial or adaptive effects of such changes within evolved populations...
January 30, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38289062/where-bacteria-and-eukaryotes-meet
#39
JOURNAL ARTICLE
Liraz Chai, Elizabeth A Shank, Vasily Zaburdaev
The international workshop "Interdisciplinary life of microbes: from single cells to multicellular aggregates," following a virtual preassembly in November 2021, was held in person in Dresden, from 9 to 13 November 2022. It attracted not only prominent experts in biofilm research but also researchers from broadly neighboring disciplines, such as medicine, chemistry, and theoretical and experimental biophysics, both eukaryotic and prokaryotic. Focused brainstorming sessions were the special feature of the event and are at the heart of this commentary...
January 30, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38289045/cell-surface-architecture-of-the-cultivated-dpann-archaeon-nanobdella-aerobiophila
#40
JOURNAL ARTICLE
Shingo Kato, Yuhei O Tahara, Yuki Nishimura, Katsuyuki Uematsu, Takahiro Arai, Daisuke Nakane, Ayaka Ihara, Takayuki Nishizaka, Wataru Iwasaki, Takashi Itoh, Makoto Miyata, Moriya Ohkuma
The DPANN archaeal clade includes obligately ectosymbiotic species. Their cell surfaces potentially play an important role in the symbiotic interaction between the ectosymbionts and their hosts. However, little is known about the mechanism of ectosymbiosis. Here, we show cell surface structures of the cultivated DPANN archaeon Nanobdella aerobiophila strain MJ1T and its host Metallosphaera sedula strain MJ1HA, using a variety of electron microscopy techniques, i.e., negative-staining transmission electron microscopy, quick-freeze deep-etch TEM, and 3D electron tomography...
January 30, 2024: Journal of Bacteriology
journal
journal
20325
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.