journal
MENU ▼
Read by QxMD icon Read
search

Journal of Bacteriology

journal
https://www.readbyqxmd.com/read/28320886/optogenetic-module-for-dichromatic-control-of-c-di-gmp-signaling
#1
Min-Hyung Ryu, Anastasia Fomicheva, Oleg V Moskvin, Mark Gomelsky
Many aspects of bacterial physiology and behavior including motility, surface attachment, and cell cycle, are controlled by the c-di-GMP-dependent signaling pathways on the scale of seconds-to-minutes. Interrogation of such processes in real time requires tools for introducing rapid and reversible changes in intracellular c-di-GMP levels. Inducing expression of genes encoding c-di-GMP synthetic (diguanylate cyclases) and degrading (c-di-GMP phosphodiesterase) enzymes by chemicals may not provide adequate temporal control...
March 20, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28320885/identification-of-the-lyso-form-n-acyl-intramolecular-transferase-in-low-gc-firmicutes
#2
Krista M Armbruster, Timothy C Meredith
Bacterial lipoproteins are embedded in the cell membrane of both Gram-positive and Gram-negative bacteria where they serve numerous functions central to cell envelope physiology. Lipoproteins are tethered to the membrane by an N-acyl-S-(mono/di)-acyl-glyceryl-cysteine anchor that is variably acylated depending on genera. In several low-GC Gram-positive Firmicutes, a monoacyl-glyceryl-cysteine with an N-terminal fatty acid (known as the lyso form) has been reported, though how it is formed is unknown. Here, through an intergenic complementation rescue assay in Escherichia coli, we report the identification of a common orthologous transmembrane protein in both Enterococcus faecalis and Bacillus cereus capable of forming lyso-form lipoproteins...
March 20, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28320884/replication-restart-in-bacteria
#3
Bénédicte Michel, Steven J Sandler
In bacteria replication forks assembled at a replication origin travel to the terminus, often a few megabases away. They may encounter obstacles that trigger replisome disassembly, rendering replication restart from abandoned forks crucial for cell viability. During the past 25 years the genes that encode replication restart proteins have been identified and genetically characterized. In parallel, the enzymes were purified and analyzed in vitro, where they can catalyze replication initiation in a sequence-independent manner from fork-like DNA structures...
March 20, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28320883/the-pseudomonas-aeruginosa-two-component-regulator-algr-directly-activates-rsma-expression-in-a-phosphorylation-independent-manner
#4
Sean D Stacey, Danielle A Williams, Christopher L Pritchett
Pseudomonas aeruginosa is an important pathogen of the immunocompromised, causing both acute and chronic infections. In cystic fibrosis (CF) patients, P. aeruginosa causes chronic disease. The impressive sensory network of P. aeruginosa allows the bacteria to sense and respond to a variety of stimuli found in diverse environments. Transcriptional regulators, including alternative sigma factors and response regulators, integrate signals changing gene expression allowing P. aeruginosa to cause infection. The two-component transcriptional regulator AlgR is important in P...
March 20, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28320882/contributions-of-the-n-terminal-and-c-terminal-domains-of-initiation-factor-3-towards-its-functions-in-the-fidelity-of-initiation-and-anti-association-of-the-ribosomal-subunits
#5
Shreya Ayyub, Divya Dobriyal, Umesh Varshney
Initiation factor 3 (IF3) is one of the three conserved prokaryotic translation initiation factors essential for protein synthesis and cellular survival. Bacterial IF3 is composed of a conserved architecture of N- and C- terminal globular domains (NTD and CTD) joined by a linker region. IF3 is a ribosome anti-association factor which also modulates selection of start codon and initiator tRNA. All the functions of IF3 have been attributed to its CTD by in vitro studies. However, in vivo relevance of these findings has not been investigated...
March 20, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28320881/lpto-pg0027-is-required-for-lipid-a-1-phosphatase-activity-in-porphyromonas-gingivalis-w50
#6
Minnie Rangarajan, Joseph Aduse-Opoku, Ahmed Hashim, Graham McPhail, Zofia Luklinska, M Florencia Haurat, Mario F Feldman, Michael A Curtis
Porphyromonas gingivalis produces outer-membrane vesicles (OMVs) rich in virulence factors including cysteine-proteases and A-LPS, one of the two lipopolysaccharides (LPS) produced by this organism. Previous studies had suggested that A-LPS together with PG0027, an outer-membrane (OM) protein, may be involved in OMV-formation. Their roles in this process were examined using parent W50 and mutant ΔPG0027 strains. Inactivation of PG0027 caused a reduction in yield of OMVs. Lipid A from cells and OMVs of P. gingivalis W50 and ΔPG0027 strains were analysed by matrix-assisted laser-desorption-ionisation-time of flight mass spectrometry (MALDI-TOF- MS)...
March 20, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28320880/rna-mediated-cis-regulation-in-acinetobacter-baumannii-modulates-stress-induced-phenotypic-variation
#7
Carly Ching, Kevin Gozzi, Björn Heinemann, Yunrong Chai, Veronica G Godoy
In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii are bimodal. Namely, there is phenotypic variation upon DNA damage, which may provide a bet-hedging strategy for survival. Thus, understanding recA gene regulation is key to elucidate the yet unknown DDR regulation in A. baumannii Here, we identify a structured 5' Untranslated Region (5' UTR) in the recA transcript which serves as a cis-regulatory element...
March 20, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28320879/metabolism-shapes-the-cell
#8
Anthony M Sperber, Jennifer K Herman
More than five decades of work support the idea that cell envelope synthesis, including the inward growth of cell division, is tightly coordinated with DNA replication and protein synthesis through central metabolism. Remarkably, no unifying model exists to account for how these fundamentally disparate processes are functionally coupled. Recent studies demonstrate that proteins involved in carbohydrate and nitrogen metabolism can moonlight as direct regulators of cell division, coordinate cell division and DNA replication, and even suppress defects in DNA replication...
March 20, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28320878/bacteria-rev-your-engines-stator-dynamics-regulate-flagellar-motility
#9
Amy E Baker, George A O'Toole
Many bacteria move through liquids and across surfaces using flagella - filaments propelled by a membrane-embedded rotary motor. Much is known about the flagellum: its basic structure, the function of its individual motor components, and the regulation of its synthesis. However, we are only beginning to identify the dynamics of flagellar proteins and to understand how the motor structurally adapts to environmental stimuli. In this review, we discuss the external and cellular factors that influence the dynamics of stator complexes (the ion-conducting channels of the flagellar motor)...
March 20, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28320877/functional-analysis-of-the-alternative-sigma-28-factor-flia-and-its-anti-sigma-factor-flgm-of-the-non-flagellated-legionella-species-l-oakridgensis
#10
Hana Tlapák, Kerstin Rydzewski, Tino Schulz, Dennis Weschka, Eva Schunder, Klaus Heuner
Legionella oakridgensis is causing the Legionnaires' disease but is known to be less virulent than Legionella pneumophilaL. oakridgensis is one of the Legionella species that is non-flagellated. The genes of the flagellar regulon are absent; except the alternative sigma-28 factor (FliA) and its anti-sigma-28 factor (FlgM). Similar to L. oakridgensis, Legionella adelaidensis and Legionella londiniensis, located in the same phylogenetic clade, they have no flagellar regulon, although both are positive for fliA and flgM Herein, we investigated the role and function of both genes to better understand the role of FliA, the positive regulator of flagellin expression, in non-flagellated strains...
March 20, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28289087/reoxidation-of-the-thiol-disulfide-oxidoreductase-mdba-by-a-bacterial-vitamin-k-epoxide-reductase-in-the-biofilm-forming-actinobacterium-actinomyces-oris
#11
Truc Thanh Luong, Melissa E Reardon-Robinson, Sara D Siegel, Hung Ton-That
Post-translocational protein folding in the Gram-positive biofilm-forming actinobacterium Actinomyces oris is mediated by a membrane-bound thiol-disulfide oxidoreductase named MdbA, which catalyzes oxidative folding of nascent polypeptides transported by the Sec translocon. Reoxidation of MdbA involves a bacterial Vitamin K Epoxide Reductase (VKOR)-like protein that contains four cysteine residues C93/C101 and C175/C178 with the latter forming a canonical CXXC thioredoxin-like motif; however, the mechanism of VKOR-mediated reoxidation of MdbA is not known...
March 13, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28289086/sgrt-a-small-protein-that-packs-a-sweet-punch
#12
Medha Raina, Gisela Storz
The SgrS small RNA (sRNA) has been shown to protect against elevated levels of glucose phosphate by regulating the stability and translation of mRNAs encoding proteins involved in sugar transport and catabolism. The sRNA also was known to encode a protective 43 amino acid protein, SgrT, but little was known about its mechanism of action. In this issue, Lloyd et al. use cell biological and genetic approaches to demonstrate that the small protein interacts with the PtsG importer to block glucose transport by this phosphotransferase system and promote utilization of non-preferred carbon sources to maintain growth during glucose-phosphate stress...
March 13, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28289085/the-small-protein-sgrt-controls-transport-activity-of-the-glucose-specific-phosphotransferase-system
#13
Chelsea R Lloyd, Seongjin Park, Jingyi Fei, Carin K Vanderpool
The bacterial small RNA (sRNA) SgrS has been a fruitful model for discovery of novel RNA-based regulatory mechanisms and new facets of bacterial physiology and metabolism. SgrS is one of only a few characterized dual-function sRNAs. SgrS can control gene expression post-transcriptionally via sRNA-mRNA base pairing interactions. Its second function is coding for the small protein SgrT. Previous work demonstrated that both functions contribute to relief of growth inhibition caused by glucose-phosphate stress, a condition characterized by disrupted glycolytic flux and accumulation of sugar-phosphates...
March 13, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28289084/control-of-recombination-directionality-by-the-listeria-phage-a118-protein-gp44-and-the-coiled-coil-motif-of-its-serine-integrase
#14
Sridhar Mandali, Kushol Gupta, Anthony R Dawson, Gregory D Van Duyne, Reid C Johnson
The serine integrase of phage A118 catalyzes integrative recombination between attP on the phage and a specific attB locus on the chromosome of Listeriamonocytogenes but is unable to promote excisive recombination between the hybrid attL and attR sites found on the integrated prophage without assistance from a Recombination Directionality Factor (RDF). We have identified and characterized the phage-encoded RDF, Gp44, which activates the A118 integrase for excision and inhibits integration. Gp44 binds to the C-terminal DNA binding domain of integrase, and we have localized the primary binding site to be within the mobile coiled-coil (CC) motif but distinct from the distal tip of the CC that is required for recombination...
March 13, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28289083/growth-tradeoffs-accompany-the-emergence-of-glycolytic-metabolism-in-shewanella-oneidensis-mr-1
#15
Lon M Chubiz, Christopher J Marx
Bacteria increase metabolic capacity via acquisition of genetic material or by mutation to genes already present in the genome. Here, we explore the mechanisms and tradeoffs involved when Shewanella oneidensis, a bacterium that typically consumes small organic and amino acids, rapidly evolves to expand its metabolic capacity to catabolize glucose after a short period of adaptation to a glucose-rich environment. Using whole-genome sequencing and genetic approaches, we discovered that deletions in a region including the transcriptional repressor (nagR) that regulates expression of genes associated with catabolism of N-acetylglucosamine is the common basis for evolved glucose metabolism across populations...
March 13, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28289082/use-of-a-phosphorylation-site-mutant-to-identify-distinct-modes-of-gene-repression-by-the-control-of-virulence-regulator-covr-in-streptococcus-pyogenes
#16
Nicola Horstmann, Pranoti Sahasrabhojane, Hui Yao, Xiaoping Su, Samuel A Shelburne
The control of virulence regulator/sensor kinase (CovRS) two-component system (TCS) serves as a model for investigating the impact of signaling pathways on the pathogenesis of Gram-positive bacteria. However the molecular mechanisms by which CovR, an OmpR/PhoB family response regulator, controls virulence gene expression are poorly defined partly due to the labile nature of its aspartate phosphorylation site. To better understand the regulatory effect of phosphorylated CovR, we generated the phosphorylation site mutant strain 10870-CovR-D53E, which we predicted to have a constitutive CovR phosphorylation phenotype...
March 13, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28289081/light-modulates-metabolic-pathways-and-other-novel-physiological-traits-in-the-human-pathogen-acinetobacter-baumannii
#17
Gabriela L Müller, Marisel Tuttobene, Matías Altilio, Maitena Martinez Amezaga, Meaghan Nguyen, P Pamela Cribb, Larisa E Cybulski, María Soledad Ramírez, Silvia Altabe, María Alejandra Mussi
Light sensing in chemotrophic bacteria has been relatively recently ascertained. In the human pathogen Acinetobacter baumannii, light modulates motility, biofilm formation and virulence through the BLUF photoreceptor BlsA. In addition, light can induce reduction in susceptibility to certain antibiotics such as minocycline and tigecycline in a photoreceptor-independent manner. In this work we identified new traits whose expression are modulated by light in this pathogen, which comprise not only important determinants related to pathogenicity and antibiotic resistance, but also metabolic pathways, which represents a novel concept for chemotrophic bacteria...
March 13, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28264995/the-dev-operon-regulates-the-timing-of-sporulation-during-myxococcus-xanthus-development
#18
Ramya Rajagopalan, Lee Kroos
Myxococcus xanthus undergoes multicellular development when starved. Thousands of rod-shaped cells coordinate their movements and aggregate into mounds in which cells differentiate into spores. Mutations in the dev operon impair development. The dev operon encompasses a clustered regularly interspaced short palindromic repeat-associated (CRISPR-Cas) system. Null mutations in devI, a small gene at the beginning of the dev operon, suppress the developmental defects caused by null mutations in the downstream devR and devS genes, but failed to suppress defects caused by a small, in-frame deletion in devT We provide evidence that the original mutant has a second-site mutation...
March 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28264994/optogenetic-manipulation-of-c-di-gmp-levels-reveals-the-role-of-c-di-gmp-in-regulating-aerotaxis-receptor-activity-in-azospirillum-brasilense
#19
Lindsey O'Neal, Min-Hyung Ryu, Mark Gomelsky, Gladys Alexandre
Bacterial chemotaxis receptors provide the sensory inputs that inform the direction of navigation in changing environments. Recently, we described the bacterial second messenger, c-di-GMP, as a novel regulator of a subclass of chemotaxis receptors. In Azospirillum brasilense, c-di-GMP binds to a chemotaxis receptor, Tlp1, and modulates its signaling function during aerotaxis. Here, we further characterize the role of c-di-GMP in aerotaxis using a novel dichromatic optogenetic system engineered for manipulating intracellular c-di-GMP levels in real time...
March 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28264993/going-outside-the-tonb-box-identification-of-novel-fepa-tonb-interactions-in-vivo
#20
Michael G Gresock, Kathleen Postle
In Gram-negative bacteria, the cytoplasmic membrane protein TonB transmits energy derived from protonmotive force to energize transport of important nutrients through TonB-dependent transporters in the outer membrane. Each transporter consists of a beta-barrel domain and a lumen-occluding cork domain containing an essential sequence called the TonB box. To date, the only identified site of transporter-TonB interaction is between the TonB box and residues ∼158-162 of TonB. While the mechanism of ligand transport is a mystery, a current model based on site-directed spin labeling and molecular dynamics simulations is that, following ligand binding, the otherwise-sequestered TonB box extends into the periplasm for recognition by TonB, which mediates transport by pulling or twisting the cork...
March 6, 2017: Journal of Bacteriology
journal
journal
20325
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"