journal
MENU ▼
Read by QxMD icon Read
search

Journal of Bacteriology

journal
https://www.readbyqxmd.com/read/29784887/identification-of-the-thioredoxin-partner-of-vkor-in-mycobacterial-disulfide-bond-formation
#1
Na Ke, Cristina Landeta, Xiaoyun Wang, Dana Boyd, Markus Eser, Jon Beckwith
Disulfide bonds influence stability and activity of many proteins. In Escherichia coli the DsbA and DsbB enzymes promote disulfide bond formation. Other bacteria, including the Actinobacteria, use instead of DsbB the enzyme VKOR (Vitamin K Epoxide Reductase), which is found either fused to or encoded in the same operon as a dsbA -like protein. Mycobacterium tuberculosis and other gram-positive Actinobacteria secrete to the cell envelope many proteins with even numbers of cysteines. These organisms have predicted oxidoreductases and VKOR orthologs...
May 21, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29784886/-legionella-pneumophila-is-directly-sensitive-to-2-deoxyglucose-phosphate-via-its-uhpc-transporter-but-is-indifferent-to-shifts-in-host-cell-glycolytic-metabolism
#2
Jordan V Price, Kallie Jiang, Abigail Galantowicz, Alana Freifeld, Russell E Vance
Toll-like receptor stimulation induces a pronounced shift to increased glycolytic metabolism in mammalian macrophages. We observed that bone marrow-derived macrophages (BMMs) increase glycolysis in response to infection with Legionella pneumophila ( L. pneumophila ), but the role of host macrophage glycolysis on intracellular L. pneumophila replication is not currently understood. Treatment with 2-deoxyglucose (2DG) blocks L. pneumophila replication in mammalian macrophages but has no effect on bacteria grown in broth...
May 21, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29784885/-pseudomonas-aeruginosa-regulated-intramembrane-proteolysis-rip-protease-mucp-can-overcome-mutations-in-the-algo-periplasmic-protease-to-restore-alginate-production-in-nonmucoid-revertants
#3
Camila Delgado, Laura Florez, Ivonne Lollett, Christine Lopez, Shiva Kangeyan, Hansi Kumari, Marios Stylianou, Robert J Smiddy, Lisa Schneper, Robert T Sautter, George Szatmari, Kalai Mathee
The progression of cystic fibrosis (CF) from an acute to a chronic disease is often associated with the conversion of the opportunistic pathogen Pseudomonas aeruginosa from a nonmucoid form to a mucoid form in the lung. This conversion involves the overproduction of the exopolysaccharide alginate, whose production is under control of the AlgT/U sigma factor. This factor is regulated posttranslationally by an extremely unstable process and has been commonly attributed to mutations in the algT/U gene. By exploiting this unstable phenotype, we isolated 34 spontaneous nonmucoid variants arising from the mucoid strain PDO300, a PAO1 derivative containing the mucA22 allele commonly found in mucoid CF isolates...
May 21, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29784884/the-effector-gene-xopae-of-xanthomonas-euvesicatoria-85-10-is-part-of-an-operon-and-encodes-an-e3-ubiquitin-ligase
#4
Georgy Popov, Bharat Bhusan Majhi, Guido Sessa
The type III effector XopAE from the Xanthomonas euvesicatoria strain 85-10 ( Xe 85-10) was previously shown to inhibit plant immunity and enhance pathogen-induced disease symptoms. Evolutionary analysis of 60 xopAE alleles ( AEal ) revealed that the xopAE locus is conserved in multiple Xanthomonas species. The majority of xopAE alleles (55 out of 60) encodes a single ORF ( xopAE ), while in 5 alleles, including AEal 37 of the Xe 85-10 strain, a frame-shift splits the locus into two ORFs ( hpaF and a truncated xopAE )...
May 21, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29784883/cytochrome-bd-oxidase-has-an-important-role-in-sustaining-growth-and-development-of-streptomyces-coelicolor-a3-2-under-oxygen-limiting-conditions
#5
Marco Fischer, Dörte Falke, Carolin Naujoks, R Gary Sawers
Streptomyces coelicolor A3 (2) is a filamentously growing, spore-forming, obligately aerobic actinobacterium that uses both a copper- aa3 - type cytochrome c oxidase and a cytochrome bd oxidase to respire oxygen. Using defined knock-out mutants we demonstrated that either of these terminal oxidases was capable of allowing the bacterium to grow and complete its developmental cycle. The genes encoding the bcc complex and the aa3 oxidase are clustered at a single locus. Using western blot analyses we showed that the bcc-aa3 oxidase branch is more prevalent than the bd oxidase in spores...
May 21, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29784882/phenotypic-assessment-suggests-multiple-start-codons-for-hetn-an-inhibitor-of-heterocyst-differentiation-in-anabaena-sp-strain-pcc-7120
#6
Orion S Rivers, Silvia Beurmann, Allexa Dow, Loralyn M Cozy, Patrick Videau
Multicellular organisms must carefully regulate the timing, number, and location of specialized cellular development. In the filamentous cyanobacterium Anabaena sp. strain PCC 7120, nitrogen-fixing heterocysts are interspersed between vegetative cells in a periodic pattern to achieve optimal exchange of bioavailable nitrogen and reduced carbon. The spacing between heterocysts is regulated by the activity of two developmental inhibitors: PatS and HetN. PatS functions to create a de novo pattern from a homogenous field of undifferentiated cells, while HetN maintains the pattern throughout subsequent growth...
May 21, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29760211/updates-to-clostridium-difficile-spore-germination
#7
Travis J Kochan, Matthew H Foley, Michelle S Shoshiev, Madeline J Somers, Paul E Carlson, Philip C Hanna
Germination of Clostridium difficile spores is a crucial early requirement for colonization of the gastrointestinal tract. Likewise, C. difficile cannot cause disease pathologies unless their spores germinate into metabolically active, toxin-producing cells. Recent advances in our understanding of C. difficile spore germination mechanisms indicate that this process is both complex and unique. This review defines unique aspects of the germination pathways of C. difficile and compares them to two other well-studied organisms, Bacillus anthracis and Clostridium perfringens C...
May 14, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29760210/the-state-of-the-union-is-strong-a-review-of-asm-s-6-th-conference-on-cell-cell-communication-in-bacteria
#8
Sam P Brown, Helen E Blackwell, Brian K Hammer
The sixth American Society for Microbiology Conference on Cell-Cell Communication in Bacteria convened from 16-19 October 2017 in Athens, GA. In this minireview, we highlight some of the research presented at that meeting that addresses central questions emerging in the field: How are cell-cell communication circuits designed to generate responses? Where are bacteria communicating? And why are bacteria engaging in such behaviors?
May 14, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29760209/complex-control-of-a-genomic-island-governing-biofilm-and-rugose-colony-development-in-vibrio-vulnificus
#9
Daniel M Chodur, Dean A Rowe-Magnus
V. vulnificus is a potent opportunistic human pathogen that contaminates the human food-chain by asymptomatically colonizing seafood. Expression of the 9-gene brp exopolysaccharide locus mediates surface adherence and is controlled by the secondary signaling molecule c-di-GMP and the regulator BrpT. Here, we show that c-di-GMP and BrpT also regulate the expression of an adjacent 5-gene cluster that includes the cabABC operon, brpT and another VpsT-like transcriptional regulator, brpS The expression of the 14 genes spanning the region increased with elevated intracellular c -di-GMP levels in a BrpT-dependent manner, save brpS , which was positively regulated by c-di-GMP and repressed by BrpT...
May 14, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29760208/rpon-dependent-direct-regulation-of-quorum-sensing-and-the-type-vi-secretion-system-in-pseudomonas-aeruginosa-pao1
#10
Xiaolong Shao, Xiaoning Zhang, Yingchao Zhang, Miao Zhu, Pan Yang, Jian Yuan, Yingpeng Xie, Tianhong Zhou, Wei Wang, Sheng Chen, Haihua Liang, Xin Deng
Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen of humans, particularly those with cystic fibrosis. As a global regulator, RpoN controls a group of virulence-related factors and quorum sensing (QS) genes in P. aeruginosa To further gain insights into the direct targets of RpoN in vivo , the present study focused on identifying the direct targets of RpoN regulation in QS and the type VI secretion system (T6SS). We performed a chromatin immunoprecipitation coupled to high-throughput sequencing (ChIP-seq) assay that identified 1,068 binding sites of RpoN, mostly including metabolic genes, a group of genes in QS ( lasI , rhlI and pqsR ) and the T6SS ( hcpA and hcpB )...
May 14, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29760207/association-of-mycobacterium-proteins-to-lipid-droplets
#11
Richard M Armstrong, Dominique C Carter, Samantha N Atkinson, Scott S Terhune, Thomas C Zahrt
Mycobacterium tuberculosis is a global pathogen of significant medical importance. A key aspect of its lifecycle is the ability to enter into an altered physiological state of non-replicating persistence during latency and resist elimination by the host immune system. One mechanism by which M. tuberculosis facilitates its survival during latency is by producing and metabolizing intracytoplasmic lipid droplets (LDs). LDs are semi-quasi organelles consisting of a neutral lipid core such as triacylglycerol surrounded by a phospholipid monolayer and proteins...
May 14, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29760206/alternative-oxidase-activity-reduces-stress-in-vibrio-fischeri-cells-exposed-to-nitric-oxide
#12
Anne K Dunn
Alternative oxidase (Aox) is a non-energy conserving respiratory oxidase found in certain eukaryotes and bacteria, whose role in physiology is not entirely clear. Using the genetically tractable bacterium Vibrio fischeri as a model organism, we have identified a role for Aox to reduce levels of stress in cells exposed to oxygen and nitric oxide (NO). In V. fischeri lacking the NO-detoxifying enzyme flavohemoglobin (Hmp), deletion of aox in cells grown in the presence of oxygen and NO results in alterations to the transcriptome that include increases in transcripts mapping to stress-related genes...
May 14, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29735765/characterization-of-flagellar-and-toxin-phase-variation-in-clostridiodes-difficile-ribotype-012-isolates
#13
Brandon R Anjuwon-Foster, Natalia Maldonado-Vazquez, Rita Tamayo
Clostridiodes difficile causes diarrheal diseases mediated in part by the secreted toxins TcdA and TcdB. C. difficile produces flagella that also contribute to motility and bacterial adherence to intestinal cells during infection. Flagellum and toxin gene expression are linked via the flagellar alternative sigma factor, SigD. Recently, we identified a "flagellar switch" upstream of the early flagellar biosynthesis operon that mediates phase variation of both flagellum and toxin production in C. difficile strain R20291...
May 7, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29735764/autoregulation-of-the-s-mutans-slor-metalloregulator-is-constitutive-and-driven-by-an-independent-promoter
#14
Patrick Monette, Richard Brach, Annie Cowan, Roger Winters, Jazz Weisman, Foster Seybert, Kelsey Goguen, James Chen, Arthur Glasfeld, Grace Spatafora
Streptococcus mutans , one of ∼600 bacterial species in the human oral cavity, is among the most acidogenic constituents of the plaque biofilm. Considered to be the primary causative agent of dental caries, S. mutans harbors a 25kDa SloR metalloregulatory protein which controls metal ion transport across the bacterial cell membrane to maintain essential metal ion homeostasis. The expression of SloR derives, in part, from transcriptional readthrough of the sloABC operon which encodes a Mn2+ /Fe2+ ABC transport system...
May 7, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29735763/diversity-and-dynamics-of-the-canadian-coastal-vibrio-community-an-emerging-trend-detected-in-the-temperate-regions
#15
Swapan K Banerjee, Rebecca Rutley, Jeff Bussey
Vibrio s pecies are indigenous to the marine and estuarine environments around the world and are the leading cause of water and seafood-borne illnesses due to conditions favouring the transmission and growth of the species. Horizontal gene transfer, recombination and mutation enable Vibrio spp to adapt rapidly to environmental challenges from biotic and abiotic parameters, including temperature, salinity and nutrient status of the coastal waters. This surveillance study provides evidence of V. cholerae emerging in the temperate estuaries of Canada, thereby redefining the diversity and dynamics of its coastal Vibrio population...
May 7, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29735762/two-dd-carboxypeptidases-from-mycobacterium-smegmatis-affect-cell-surface-properties-through-regulation-of-peptidoglycan-cross-linking-and-glycopeptidolipids
#16
Satya Deo Pandey, Shilpa Pal, Ganesh Kumar N, Ankita Bansal, Sathi Mallick, Anindya S Ghosh
During the peptidoglycan (PG) maturation of mycobacteria, the glycan strands are interlinked by both 3-3 (between two meso-DAP) and 4-3 cross-links (between D-ala and meso-DAP), though there is a predominance (60-80%) of 3-3 cross-links. The DD-CPases act on pentapeptides to generate tetrapeptides that are used by LD-transpeptidases as substrates to form 3-3 cross-links. Therefore, DD-CPases play a crucial role in mycobacterial PG cross-link formation. However, the physiology of DD-CPases in mycobacteria is relatively unexplored...
May 7, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29735761/metabolic-reprogramming-of-vibrio-cholerae-impaired-in-respiratory-nadh-oxidation-is-accompanied-with-increased-copper-sensitivity
#17
Charlotte Toulouse, Kristina Metesch, Jens Pfannstiel, Julia Steuber
The electrogenic, sodium ion translocating NADH:quinone oxidoreductase (NQR) from Vibrio cholerae is frequent in pathogenic bacteria and a potential target for antibiotics. NQR couples the oxidation of NADH to the formation of a sodium motive force (SMF) and therefore drives important processes such as flagellar rotation, substrate uptake, and energy-dissipating cation-proton antiport. We performed a quantitative proteome analysis of V. cholerae O395N1 in comparison to its variant lacking the NQR using minimal medium with glucose as carbon source...
May 7, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29735760/disruption-of-a-novel-iron-transport-system-reverses-oxidative-stress-phenotypes-of-a-dpr-mutant-strain-of-streptococcus-mutans
#18
Tridib Ganguly, Jessica K Kajfasz, James H Miller, Eric Rabinowitz, Lívia C C Galvão, Pedro L Rosalen, Jacqueline Abranches, José A Lemos
The Dps-like peroxide resistance protein (Dpr) is essential for H2 O2 stress tolerance and aerobic growth of the oral pathogen Streptococcus mutans Dpr accumulates during oxidative stress, protecting the cell by sequestering iron ions, thereby preventing the generation of toxic hydroxyl radicals that result from the interaction of iron with H2 O2 Previously, we reported that the SpxA1 and SpxA2 regulators positively regulate expression of dpr in S. mutans Using an antibody raised against S. mutans Dpr, we confirmed at the protein level the central and cooperative nature of SpxA1 and SpxA2 regulation in Dpr production...
May 7, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29735759/measurement-of-internal-ph-in-helicobacter-pylori-by-using-green-fluorescent-protein-fluorimetry
#19
Yi Wen, David R Scott, Olga Vagin, Elmira Tokhtaeva, Elizabeth A Marcus, George Sachs
Helicobacter pylori is an organism known to colonize the normal human stomach. Previous studies have shown that the bacterium does this by elevating its periplasmic pH via the hydrolysis of urea. However, the value of the periplasmic pH was calculated indirectly from the proton motive force equation. To measure the periplasmic pH directly in H. pylori , we fused EGFP to the predicted twin arginine-signal peptides of HydA and KapA from H. pylori , and TorA from E. coli The fusion proteins were expressed in the H...
May 7, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29735758/the-impact-of-active-metabolism-on-chlamydia-trachomatis-elementary-body-transcript-profile-and-infectivity
#20
Scott Grieshaber, Nicole Grieshaber, Hong Yang, Briana Baxter, Ted Hackstadt, Anders Omsland
Bacteria of the genus Chlamydia include the significant human pathogens C. trachomatis and C. pneumoniae All Chlamydiae are obligate intracellular parasites that depend on infection of a host cell and transition through a biphasic developmental cycle. Following host cell invasion by the infectious Elementary Body (EB), the pathogen transitions to the replicative but non-infectious Reticulate Body (RB). Differentiation of the RB back to the EB is essential to generate infectious progeny. While the EB form has historically been regarded as metabolically inert, maintenance of infectivity during incubation with specific nutrients has revealed active maintenance of the infectious phenotype...
May 7, 2018: Journal of Bacteriology
journal
journal
20325
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"