journal
https://read.qxmd.com/read/38531364/brucella-driven-host-n-glycome-remodeling-controls-infection
#1
JOURNAL ARTICLE
Ana-Lucia Cabello, Kelsey Wells, Wenjing Peng, Hui-Qiang Feng, Junyao Wang, Damien F Meyer, Christophe Noroy, En-Shuang Zhao, Hao Zhang, Xueqing Li, Haowu Chang, Gabriel Gomez, Yuxin Mao, Kristin L Patrick, Robert O Watson, William K Russell, Aiying Yu, Jieqiang Zhong, Fengguang Guo, Mingqian Li, Mingyuan Zhou, Xiaoning Qian, Koichi S Kobayashi, Jianxun Song, Suresh Panthee, Yehia Mechref, Thomas A Ficht, Qing-Ming Qin, Paul de Figueiredo
Many powerful methods have been employed to elucidate the global transcriptomic, proteomic, or metabolic responses to pathogen-infected host cells. However, the host glycome responses to bacterial infection remain largely unexplored, and hence, our understanding of the molecular mechanisms by which bacterial pathogens manipulate the host glycome to favor infection remains incomplete. Here, we address this gap by performing a systematic analysis of the host glycome during infection by the bacterial pathogen Brucella spp...
March 20, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38513657/a-specific-enterotype-derived-from-gut-microbiome-of-older-individuals-enables-favorable-responses-to-immune-checkpoint-blockade-therapy
#2
JOURNAL ARTICLE
Xiaoqiang Zhu, Xiaowen Huang, Muni Hu, Rongrong Sun, Jiantao Li, Hai Wang, Xuefeng Pan, Yanru Ma, Lijun Ning, Tianying Tong, Yilu Zhou, Jinmei Ding, Ying Zhao, Baoqin Xuan, Jing-Yuan Fang, Jie Hong, Jason Wing Hon Wong, Youwei Zhang, Haoyan Chen
Immunotherapy has revolutionized cancer treatment, but inconsistent responses persist. Our study delves into the intriguing phenomenon of enhanced immunotherapy sensitivity in older individuals with cancers. Through a meta-analysis encompassing 25 small-to-mid-sized trials of immune checkpoint blockade (ICB), we demonstrate that older individuals exhibit heightened responsiveness to ICB therapy. To understand the underlying mechanism, we reanalyze single-cell RNA sequencing (scRNA-seq) data from multiple studies and unveil distinct upregulation of exhausted and cytotoxic T cell markers within the tumor microenvironment (TME) of older patients...
March 18, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38513656/opposing-diet-microbiome-and-metabolite-mechanisms-regulate-inflammatory-bowel-disease-in-a-genetically-susceptible-host
#3
JOURNAL ARTICLE
Gabriel Vasconcelos Pereira, Marie Boudaud, Mathis Wolter, Celeste Alexander, Alessandro De Sciscio, Erica T Grant, Bruno Caetano Trindade, Nicholas A Pudlo, Shaleni Singh, Austin Campbell, Mengrou Shan, Li Zhang, Qinnan Yang, Stéphanie Willieme, Kwi Kim, Trisha Denike-Duval, Jaime Fuentes, André Bleich, Thomas M Schmidt, Lucy Kennedy, Costas A Lyssiotis, Grace Y Chen, Kathryn A Eaton, Mahesh S Desai, Eric C Martens
Inflammatory bowel diseases (IBDs) are chronic conditions characterized by periods of spontaneous intestinal inflammation and are increasing in industrialized populations. Combined with host genetics, diet and gut bacteria are thought to contribute prominently to IBDs, but mechanisms are still emerging. In mice lacking the IBD-associated cytokine, interleukin-10, we show that a fiber-deprived gut microbiota promotes the deterioration of colonic mucus, leading to lethal colitis. Inflammation starts with the expansion of natural killer cells and altered immunoglobulin-A coating of some bacteria...
March 18, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38513655/cytoplasmic-calcium-influx-mediated-by-plant-mlkls-confers-tnl-triggered-immunity
#4
JOURNAL ARTICLE
Qiaochu Shen, Keiichi Hasegawa, Nicole Oelerich, Anna Prakken, Lea Weiler Tersch, Junli Wang, Frowin Reichhardt, Alexandra Tersch, Je Cuan Choo, Ton Timmers, Kay Hofmann, Jane E Parker, Jijie Chai, Takaki Maekawa
The plant homolog of vertebrate necroptosis inducer mixed-lineage kinase domain-like (MLKL) contributes to downstream steps in Toll-interleukin-1 receptor domain NLR (TNL)-receptor-triggered immunity. Here, we show that Arabidopsis MLKL1 (AtMLKL1) clusters into puncta at the plasma membrane upon TNL activation and that this sub-cellular reorganization is dependent on the TNL signal transducer, EDS1. We find that AtMLKLs confer TNL-triggered immunity in parallel with RPW8-type HeLo-domain-containing NLRs (RNLs) and that the AtMLKL N-terminal HeLo domain is indispensable for both immunity and clustering...
March 13, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38479397/longitudinal-profiling-of-the-microbiome-at-four-body-sites-reveals-core-stability-and-individualized-dynamics-during-health-and-disease
#5
JOURNAL ARTICLE
Xin Zhou, Xiaotao Shen, Jethro S Johnson, Daniel J Spakowicz, Melissa Agnello, Wenyu Zhou, Monica Avina, Alexander Honkala, Faye Chleilat, Shirley Jingyi Chen, Kexin Cha, Shana Leopold, Chenchen Zhu, Lei Chen, Lin Lyu, Daniel Hornburg, Si Wu, Xinyue Zhang, Chao Jiang, Liuyiqi Jiang, Lihua Jiang, Ruiqi Jian, Andrew W Brooks, Meng Wang, Kévin Contrepois, Peng Gao, Sophia Miryam Schüssler-Fiorenza Rose, Thi Dong Binh Tran, Hoan Nguyen, Alessandra Celli, Bo-Young Hong, Eddy J Bautista, Yair Dorsett, Paula B Kavathas, Yanjiao Zhou, Erica Sodergren, George M Weinstock, Michael P Snyder
To understand the dynamic interplay between the human microbiome and host during health and disease, we analyzed the microbial composition, temporal dynamics, and associations with host multi-omics, immune, and clinical markers of microbiomes from four body sites in 86 participants over 6 years. We found that microbiome stability and individuality are body-site specific and heavily influenced by the host. The stool and oral microbiome are more stable than the skin and nasal microbiomes, possibly due to their interaction with the host and environment...
March 8, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38479396/pathophysiology-of-chikungunya-virus-infection-associated-with-fatal-outcomes
#6
JOURNAL ARTICLE
William M de Souza, Marcilio J Fumagalli, Shirlene T S de Lima, Pierina L Parise, Deyse C M Carvalho, Cristian Hernandez, Ronaldo de Jesus, Jeany Delafiori, Darlan S Candido, Victor C Carregari, Stefanie P Muraro, Gabriela F Souza, Leda M Simões Mello, Ingra M Claro, Yamilka Díaz, Rodrigo B Kato, Lucas N Trentin, Clauber H S Costa, Ana Carolina B M Maximo, Karene F Cavalcante, Tayna S Fiuza, Vânia A F Viana, Maria Elisabeth L Melo, Clarissa P M Ferraz, Débora B Silva, Larissa M F Duarte, Priscilla P Barbosa, Mariene R Amorim, Carla C Judice, Daniel A Toledo-Teixeira, Mariana S Ramundo, Patricia V Aguilar, Emerson L L Araújo, Fabio T M Costa, Thiago Cerqueira-Silva, Ricardo Khouri, Viviane S Boaventura, Luiz Tadeu M Figueiredo, Rong Fang, Brechla Moreno, Sandra López-Vergès, Liana Perdigão Mello, Munir S Skaf, Rodrigo R Catharino, Fabiana Granja, Daniel Martins-de-Souza, Jessica A Plante, Kenneth S Plante, Ester C Sabino, Michael S Diamond, Eliseo Eugenin, José Luiz Proença-Módena, Nuno R Faria, Scott C Weaver
Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes acute, subacute, and chronic human arthritogenic diseases and, in rare instances, can lead to neurological complications and death. Here, we combined epidemiological, virological, histopathological, cytokine, molecular dynamics, metabolomic, proteomic, and genomic analyses to investigate viral and host factors that contribute to chikungunya-associated (CHIK) death. Our results indicate that CHIK deaths are associated with multi-organ infection, central nervous system damage, and elevated serum levels of pro-inflammatory cytokines and chemokines compared with survivors...
March 8, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38479394/sugar-transporters-spatially-organize-microbiota-colonization-along-the-longitudinal-root-axis-of-arabidopsis
#7
JOURNAL ARTICLE
Eliza P-I Loo, Paloma Durán, Tin Yau Pang, Philipp Westhoff, Chen Deng, Carlos Durán, Martin Lercher, Ruben Garrido-Oter, Wolf B Frommer
Plant roots are functionally heterogeneous in cellular architecture, transcriptome profile, metabolic state, and microbial immunity. We hypothesized that axial differentiation may also impact spatial colonization by root microbiota along the root axis. We developed two growth systems, ArtSoil and CD-Rhizotron, to grow and then dissect Arabidopsis thaliana roots into three segments. We demonstrate that distinct endospheric and rhizosphere bacterial communities colonize the segments, supporting the hypothesis of microbiota differentiation along the axis...
March 8, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38479393/stratification-of-fusobacterium-nucleatum-by-local-health-status-in-the-oral-cavity-defines-its-subspecies-disease-association
#8
JOURNAL ARTICLE
Madeline Krieger, Yasser M AbdelRahman, Dongseok Choi, Elizabeth A Palmer, Anna Yoo, Sean McGuire, Jens Kreth, Justin Merritt
The ubiquitous inflammophilic oral pathobiont Fusobacterium nucleatum (Fn) is widely recognized for its strong association with inflammatory dysbiotic diseases and cancer. Fn is subdivided into four subspecies, which are historically considered functionally interchangeable in the oral cavity. To test this assumption, we analyzed patient-matched dental plaque and odontogenic abscess clinical specimens and examined whether an inflammatory environment selects for/against particular Fn subspecies. Dental plaque harbored a greater diversity of fusobacteria, with Fn...
March 8, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38479395/human-cytomegalovirus-degrades-dmxl1-to-inhibit-autophagy-lysosomal-acidification-and-viral-assembly
#9
JOURNAL ARTICLE
Hanqi Li, Alice Fletcher-Etherington, Leah M Hunter, Swati Keshri, Ceri A Fielding, Katie Nightingale, Benjamin Ravenhill, Luis Nobre, Martin Potts, Robin Antrobus, Colin M Crump, David C Rubinsztein, Richard J Stanton, Michael P Weekes
Human cytomegalovirus (HCMV) is an important human pathogen that regulates host immunity and hijacks host compartments, including lysosomes, to assemble virions. We combined a quantitative proteomic analysis of HCMV infection with a database of proteins involved in vacuolar acidification, revealing Dmx-like protein-1 (DMXL1) as the only protein that acidifies vacuoles yet is degraded by HCMV. Systematic comparison of viral deletion mutants reveals the uncharacterized 7 kDa US33A protein as necessary and sufficient for DMXL1 degradation, which occurs via recruitment of the E3 ubiquitin ligase Kip1 ubiquitination-promoting complex (KPC)...
March 5, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38417443/a-conserved-antigen-induces-respiratory-th17-mediated-broad-serotype-protection-against-pneumococcal-superinfection
#10
JOURNAL ARTICLE
Xue Liu, Laurye Van Maele, Laura Matarazzo, Daphnée Soulard, Vinicius Alves Duarte da Silva, Vincent de Bakker, Julien Dénéréaz, Florian P Bock, Michael Taschner, Jinzhao Ou, Stephan Gruber, Victor Nizet, Jean-Claude Sirard, Jan-Willem Veening
Several vaccines targeting bacterial pathogens show reduced efficacy upon concurrent viral infection, indicating that a new vaccinology approach is required. To identify antigens for the human pathogen Streptococcus pneumoniae that are effective following influenza infection, we performed CRISPRi-seq in a murine model of superinfection and identified the conserved lafB gene as crucial for virulence. We show that LafB is a membrane-associated, intracellular protein that catalyzes the formation of galactosyl-glucosyl-diacylglycerol, a glycolipid important for cell wall homeostasis...
February 21, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38402614/bacterial-defense-systems-exhibit-synergistic-anti-phage-activity
#11
JOURNAL ARTICLE
Yi Wu, Sofya K Garushyants, Anne van den Hurk, Cristian Aparicio-Maldonado, Simran Krishnakant Kushwaha, Claire M King, Yaqing Ou, Thomas C Todeschini, Martha R J Clokie, Andrew D Millard, Yilmaz Emre Gençay, Eugene V Koonin, Franklin L Nobrega
Bacterial defense against phage predation involves diverse defense systems acting individually and concurrently, yet their interactions remain poorly understood. We investigated >100 defense systems in 42,925 bacterial genomes and identified numerous instances of their non-random co-occurrence and negative association. For several pairs of defense systems significantly co-occurring in Escherichia coli strains, we demonstrate synergistic anti-phage activity. Notably, Zorya II synergizes with Druantia III and ietAS defense systems, while tmn exhibits synergy with co-occurring systems Gabija, Septu I, and PrrC...
February 19, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38412863/alistipes-indistinctus-derived-hippuric-acid-promotes-intestinal-urate-excretion-to-alleviate-hyperuricemia
#12
JOURNAL ARTICLE
Ying-Xi Xu, Lu-Di Liu, Jiang-Yuan Zhu, Shan-Shan Zhu, Bing-Qi Ye, Jia-Lu Yang, Jing-Yi Huang, Zhi-Hao Huang, Yi You, Wen-Kang Li, Jia-Lin He, Min Xia, Yan Liu
Hyperuricemia induces inflammatory arthritis and accelerates the progression of renal and cardiovascular diseases. Gut microbiota has been linked to the development of hyperuricemia through unclear mechanisms. Here, we show that the abundance and centrality of Alistipes indistinctus are depleted in subjects with hyperuricemia. Integrative metagenomic and metabolomic analysis identified hippuric acid as the key microbial effector that mediates the uric-acid-lowering effect of A. indistinctus. Mechanistically, A...
February 16, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38367621/donor-recipient-intermicrobial-interactions-impact-transfer-of-subspecies-and-fecal-microbiota-transplantation-outcome
#13
JOURNAL ARTICLE
Qiyi Chen, Chunyan Wu, Jinfeng Xu, Chen Ye, Xiang Chen, Hongliang Tian, Naixin Zong, Shaoyi Zhang, Long Li, Yuan Gao, Di Zhao, Xiaoqiong Lv, Qilin Yang, Le Wang, Jiaqu Cui, Zhiliang Lin, Jubao Lu, Rong Yang, Fang Yin, Nan Qin, Ning Li, Qian Xu, Huanlong Qin
Studies on fecal microbiota transplantation (FMT) have reported inconsistent connections between clinical outcomes and donor strain engraftment. Analyses of subspecies-level crosstalk and its influences on lineage transfer in metagenomic FMT datasets have proved challenging, as single-nucleotide polymorphisms (SNPs) are generally not linked and are often absent. Here, we utilized species genome bin (SGB), which employs co-abundance binning, to investigate subspecies-level microbiome dynamics in patients with autism spectrum disorder (ASD) who had gastrointestinal comorbidities and underwent encapsulated FMT (Chinese Clinical Trial: 2100043906)...
February 12, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38377995/xbb-1-5-monovalent-mrna-vaccine-booster-elicits-robust-neutralizing-antibodies-against-xbb-subvariants-and-jn-1
#14
JOURNAL ARTICLE
Qian Wang, Yicheng Guo, Anthony Bowen, Ian A Mellis, Riccardo Valdez, Carmen Gherasim, Aubree Gordon, Lihong Liu, David D Ho
COVID-19 vaccines have recently been updated to specifically encode or contain the spike protein of the SARS-CoV-2 XBB.1.5 subvariant, but their immunogenicity in humans has yet to be fully evaluated and reported, particularly against emergent viruses that are rapidly expanding. We now report that administration of an updated monovalent mRNA vaccine booster (XBB.1.5 MV) to previously uninfected individuals boosted serum virus-neutralizing antibodies significantly against not only XBB.1.5 (27.0-fold increase) and EG...
February 10, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38359828/susceptible-bacteria-can-survive-antibiotic-treatment-in-the-mammalian-gastrointestinal-tract-without-evolving-resistance
#15
JOURNAL ARTICLE
Marinelle Rodrigues, Parastoo Sabaeifard, Muhammed Sadik Yildiz, Adam Lyon, Laura Coughlin, Sara Ahmed, Nicole Poulides, Ahmet C Toprak, Cassie Behrendt, Xiaoyu Wang, Marguerite Monogue, Jiwoong Kim, Shuheng Gan, Xiaowei Zhan, Laura Filkins, Noelle S Williams, Lora V Hooper, Andrew Y Koh, Erdal Toprak
Antibiotic resistance and evasion are incompletely understood and complicated by the fact that murine interval dosing models do not fully recapitulate antibiotic pharmacokinetics in humans. To better understand how gastrointestinal bacteria respond to antibiotics, we colonized germ-free mice with a pan-susceptible genetically barcoded Escherichia coli clinical isolate and administered the antibiotic cefepime via programmable subcutaneous pumps, allowing closer emulation of human parenteral antibiotic dynamics...
February 6, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38309260/dgk5%C3%AE-derived-phosphatidic-acid-regulates-ros-production-in-plant-immunity-by-stabilizing-nadph-oxidase
#16
JOURNAL ARTICLE
Fan Qi, Jianwei Li, Yingfei Ai, Keke Shangguan, Ping Li, Fucheng Lin, Yan Liang
In plant immunity, phosphatidic acid (PA) regulates reactive oxygen species (ROS) by binding to respiratory burst oxidase homolog D (RBOHD), an NADPH oxidase responsible for ROS production. Here, we analyze the influence of PA binding on RBOHD activity and the mechanism of RBOHD-bound PA generation. PA binding enhances RBOHD protein stability by inhibiting vacuolar degradation, thereby increasing chitin-induced ROS production. Mutations in diacylglycerol kinase 5 (DGK5), which phosphorylates diacylglycerol to produce PA, impair chitin-induced PA and ROS production...
February 2, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38307019/evolution-of-human-h3n2-influenza-virus-receptor-specificity-has-substantially-expanded-the-receptor-binding-domain-site
#17
JOURNAL ARTICLE
Andrew J Thompson, Nicholas C Wu, Angeles Canales, Chika Kikuchi, Xueyong Zhu, Beatriz Fernández de Toro, Francisco J Cañada, Charli Worth, Shengyang Wang, Ryan McBride, Wenjie Peng, Corwin M Nycholat, Jesús Jiménez-Barbero, Ian A Wilson, James C Paulson
Hemagglutinins (HAs) from human influenza viruses descend from avian progenitors that bind α2-3-linked sialosides and must adapt to glycans with α2-6-linked sialic acids on human airway cells to transmit within the human population. Since their introduction during the 1968 pandemic, H3N2 viruses have evolved over the past five decades to preferentially recognize human α2-6-sialoside receptors that are elongated through addition of poly-LacNAc. We show that more recent H3N2 viruses now make increasingly complex interactions with elongated receptors while continuously selecting for strains maintaining this phenotype...
January 29, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38309259/the-live-biotherapeutic-synb1353-decreases-plasma-methionine-via-directed-degradation-in-animal-models-and-healthy-volunteers
#18
JOURNAL ARTICLE
Mylène Perreault, Jillian Means, Erik Gerson, Michael James, Sean Cotton, Christopher G Bergeron, Mark Simon, Dylan Alexander Carlin, Nathan Schmidt, Theodore C Moore, Julie Blasbalg, Neal Sondheimer, Kenneth Ndugga-Kabuye, William S Denney, Vincent M Isabella, David Lubkowicz, Aoife Brennan, David L Hava
Methionine is an essential proteinogenic amino acid, but its excess can lead to deleterious effects. Inborn errors of methionine metabolism resulting from loss of function in cystathionine β-synthase (CBS) cause classic homocystinuria (HCU), which is managed by a methionine-restricted diet. Synthetic biotics are gastrointestinal tract-targeted live biotherapeutics that can be engineered to replicate the benefits of dietary restriction. In this study, we assess whether SYNB1353, an E. coli Nissle 1917 derivative, impacts circulating methionine and homocysteine levels in animals and healthy volunteers...
January 25, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38307020/anaerobic-respiration-of-host-derived-methionine-sulfoxide-protects-intracellular-salmonella-from-the-phagocyte-nadph-oxidase
#19
JOURNAL ARTICLE
Ju-Sim Kim, Lin Liu, Sashi Kant, David J Orlicky, Siva Uppalapati, Alyssa Margolis, Bennett J Davenport, Thomas E Morrison, Jennifer Matsuda, Michael McClelland, Jessica Jones-Carson, Andres Vazquez-Torres
Intracellular Salmonella experiencing oxidative stress downregulates aerobic respiration. To maintain cellular energetics during periods of oxidative stress, intracellular Salmonella must utilize terminal electron acceptors of lower energetic value than molecular oxygen. We show here that intracellular Salmonella undergoes anaerobic respiration during adaptation to the respiratory burst of the phagocyte NADPH oxidase in macrophages and in mice. Reactive oxygen species generated by phagocytes oxidize methionine, generating methionine sulfoxide...
January 24, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38295788/intestinal-microbiota-programming-of-alveolar-macrophages-influences-severity-of-respiratory-viral-infection
#20
JOURNAL ARTICLE
Vu L Ngo, Carolin M Lieber, Hae-Ji Kang, Kaori Sakamoto, Michal Kuczma, Richard K Plemper, Andrew T Gewirtz
Susceptibility to respiratory virus infections (RVIs) varies widely across individuals. Because the gut microbiome impacts immune function, we investigated the influence of intestinal microbiota composition on RVI and determined that segmented filamentous bacteria (SFB), naturally acquired or exogenously administered, protected mice against influenza virus (IAV) infection. Such protection, which also applied to respiratory syncytial virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was independent of interferon and adaptive immunity but required basally resident alveolar macrophages (AMs)...
January 23, 2024: Cell Host & Microbe
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