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Cell Transplantation

Yoshiki Nakashima, Saifun Nahar, Chika Miyagi-Shiohira, Takao Kinjo, Zensei Toyoda, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Jiro Fujita, Hirofumi Noguchi
Liquid chromatography using a tandem mass spectrometer (LC-MS/MS) is a method of proteomic analysis. A shotgun analysis by LC-MS/MS comprehensively identifies proteins from tissues and cells with high resolution. The hepatic function of mice with acute hepatitis following the intraperitoneal administration of CCL4 was improved by the tail vein administration of the culture conditional medium (CM) of human mesenchymal stem cells from adipose tissue (hMSC-AT). In this study, a secreted protein expression analysis of hMSC-AT was performed using LC-MS/MS; 128 proteins were identified...
September 18, 2018: Cell Transplantation
Dong-Won Seol, Seah Park, Eun Young Shin, Jae Ho Chang, Dong Ryul Lee
Sertoli cells (SCs) in the mammalian testes are well known as supporting cells of spermatogenesis, but have recently become an attractive source of cell therapy because of their capacity for immune modulation and trophic effects. In order to increase their applicable efficacy, we demonstrate a novel differentiation method for mouse embryonic stem cell (ESC)-derived Sertoli-like cells (SLCs) via the intermediate mesoderm (IM). We show that IM derived from an induction of 6 days expressed markers such as Wt1, Lhx1, Pax2 and Osr1, and that a sequential induction of 6 days resulted in ESC-SLCs...
September 14, 2018: Cell Transplantation
Mark J Stice, Ty B Dunn, Melena D Bellin, Mariya E Skube, Greg J Beilman
Total pancreatectomy and islet autotransplantation (TPIAT) is an effective treatment for selected patients with chronic pancreatitis. The portal circulation is the standard infusion site for islet transplant, but marked elevation of portal pressures may prevent complete islet infusion. Herein we report a novel technique of combined site islet autotransplantation using an omental pouch. This technique may be useful when technical limitations prevent complete intraportal transplantation. In four TPIAT recipients with intraoperative issues precluding the complete intraportal infusion of islets, an omental pouch was created to contain the remaining islet mass...
September 14, 2018: Cell Transplantation
Serdar Kabataş, Erdinç Civelek, Çiğdem İnci, Ebru Yılmaz Yalçınkaya, Gülşen Günel, Gülay Kır, Esra Albayrak, Erek Öztürk, Gökhan Adaş, Erdal Karaöz
Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) have been introduced as a possible therapy in hypoxic-ischemic encephalopathy (HIE). We report a 16-year-old boy who was treated with WJ-MSCs in the course of HIE due to post-cardiopulmonary resuscitation. He received a long period of mechanical ventilation and tracheostomy with spastic quadriparesis. He underwent the intrathecal (1×106 /kg in 3 mL), intramuscular (1×106 /kg in 20 mL) and intravenous (1×106 /kg in 30 mL) administrations of WJ-MSCs for each application route (twice a month for 2 months)...
September 11, 2018: Cell Transplantation
Pavel Šponer, Tomáš Kučera, Jindra Brtková, Karel Urban, Zuzana Kočí, Pavel Měřička, Aleš Bezrouk, Šimona Konrádová, Alžběta Filipová, Stanislav Filip
This prospective study sought to evaluate the healing quality of implanted ultraporous β-tricalcium phosphate sown with expanded autologous mesenchymal stromal cells (MSCs) into femoral defects during revision hip arthroplasty. A total of 37 osseous defects in 37 patients were treated and evaluated concerning bone regeneration. Nineteen subjects received β-tricalcium phosphate graft material serving as a carrier of expanded autologous MSCs (the trial group A), nine subjects received β-tricalcium phosphate graft material only (the study group B) and nine subjects received cancellous allografts only (the control group C)...
September 11, 2018: Cell Transplantation
Amer Rajab, Jill Buss, Phil A Hart, Darwin Conwell, Luis Lara, Shumei Meng, Kristin Kuntz, Sylvester Black, Ken Washburn
Hepatic parenchymal disease, including chronic viral hepatitis, has traditionally been considered a relative contraindication to islet transplantation as the islets are infused into the recipient's liver. We present a case study of a patient with treated chronic hepatitis C infection (HCV) who safely received an autologous islet transplant following total pancreatectomy with excellent clinical outcomes. The patient was a 60-year-old woman diagnosed with debilitating abdominal pain secondary to chronic pancreatitis and with preserved islet function...
September 11, 2018: Cell Transplantation
Xin Zhong, Yonghua Zheng, Hua Li, Steve Huang, Junbo Ge
OBJECTIVES: The aim of this study was to compare the morphology, immune phenotype, and cytokine profiles between myocardial telocytes (TCs) and bone marrow mesenchymal stem cells (MSCs), and explore the difference between those two types of interstitial cells. METHODS: TCs and MSCs were cultured in vitro and cell morphology was observed with a light microscope. The expression levels of CD34, c-kit, and vimentin were detected by immunofluorescence, RT-qPCR, and Western blotting in both TCs and MSCs...
September 11, 2018: Cell Transplantation
Niann-Tzyy Dai, Wen-Shyan Huang, Fang-Wei Chang, Lin-Gwei Wei, Tai-Chun Huang, Jhen-Kai Li, Keng-Yen Fu, Lien-Guo Dai, Pai-Shan Hsieh, Nien-Chi Huang, Yi-Wen Wang, Hsin-I Chang, Roxanne Parungao, Yiwei Wang
Skin substitutes with existing vascularization are in great demand for the repair of full-thickness skin defects. In the present study, we hypothesized that a pre-vascularized skin substitute can potentially promote wound healing. Novel three-dimensional (3D) skin substitutes were prepared by seeding a mixture of human endothelial progenitor cells (EPCs) and fibroblasts into a human plasma/calcium chloride formed gel scaffold, and seeding keratinocytes onto the surface of the plasma gel. The capacity of the EPCs to differentiate into a vascular-like tubular structure was evaluated using immunohistochemistry analysis and WST-8 assay...
September 11, 2018: Cell Transplantation
Y Meng, A Eirin, X-Y Zhu, H Tang, L J Hickson, A Lerman, A J van Wijnen, L O Lerman
Mesenchymal stem cells (MSCs) constitute an important repair system, but may be impaired by exposure to cardiovascular risk factors. Consequently, adipose tissue-derived MSCs from pigs with the metabolic syndrome (MetS) show decreased vitality. A growing number of microRNAs (miRNAs) are recognized as key modulators of senescence, but their role in regulating senescence in MSC in MetS is unclear. We tested the hypothesis that MetS upregulates in MSC expression of miRNAs that can serve as post-transcriptional regulators of senescence-associated (SA) genes...
September 6, 2018: Cell Transplantation
Wenzhi Ma, Jia Wang, Weijun Gao, Hua Jia
Safety is the chief consideration in recipient preparation of spermatogonial stem cell (SSC) transplantation in mammals, especially humans. In this study, we compared the safety of the SSC transplantation recipients that were prepared both by testes heat shock plus testes busulfan injection (heat shock+busulfan(t)) and by busulfan intraperitoneal injection (busulfan i.p.) only. Our results showed that heat shock+busulfan(t) treatment significantly ( p < 0.05) reduced mortality in mice and did not produce bone marrow cell toxicity...
September 6, 2018: Cell Transplantation
Yuan-Chuan Chen, Ching-Yi Lee, Shiu-Jau Chen
Back pain is a common health problem that reduces the quality of life for human beings worldwide. Several treatment modalities have been reported as effective for pain relief. Generally, patients often undergo surgical interventions as pain becomes intractable, after conservative treatment. With advances in surgical techniques, those choosing spinal surgery as an option have increased over time, and instrumentation is more popular than it was years ago. However, some patients still have back pain after spinal operations...
August 31, 2018: Cell Transplantation
Jinying Li, Chen Qiu, Zheng Zhang, Weixin Yuan, Zhen Ge, Bing Tan, Pengjie Yang, Jia Liu, Xiaolong Zhu, Cong Qiu, Dongmei Lai, Lihe Guo, Luyang Yu
As a featured ocular inflammatory disease, autoimmune uveitis is the major cause of blindness in the clinic. Although current immunosuppressive regimens can alleviate the progression of autoimmune uveitis, they have serious side effects. Therefore, an alternative therapeutic strategy is urgently required. The present study investigated the therapeutic efficacy of human amniotic epithelial cells (hAECs) on autoimmune uveitis in a rat model. Herein, experimental autoimmune uveitis (EAU) was induced in rats via a subcutaneous injection of interphotoreceptor retinoid-binding protein...
August 31, 2018: Cell Transplantation
Poornima Venkat, Tao Yan, Michael Chopp, Alex Zacharek, Ruizhuo Ning, Paul Van Slyke, Daniel Dumont, Julie Landschoot-Ward, Linlin Liang, Jieli Chen
BACKGROUND AND OBJECTIVE: Angiopoietin-1 (Ang1) mediates vascular maturation and immune response. Diabetes decreases Ang1 expression and disrupts Ang1/Tie2 signaling activity. Vasculotide is an Ang1 mimetic peptide, and has anti-inflammatory effects. In this study, we test the hypothesis that vasculotide treatment induces neuroprotection and decreases inflammation after stroke in type 1 diabetic (T1DM) rats. METHODS: T1DM rats were subjected to embolic middle cerebral artery occlusion (MCAo) and treated with: 1) phosphate buffered saline (PBS); 2) vasculotide (3µg/kg, i...
August 20, 2018: Cell Transplantation
Mehmet H Kural, Guohao Dai, Laura E Niklason, Liqiong Gui
OBJECTIVE: Invasive coronary interventions can fail due to intimal hyperplasia and restenosis. Endothelial cell (EC) seeding to the vessel lumen, accelerating re-endothelialization, or local release of mTOR pathway inhibitors have helped reduce intimal hyperplasia after vessel injury. While animal models are powerful tools, they are complex and expensive, and not always reflective of human physiology. Therefore, we developed an in vitro 3D vascular model validating previous in vivo animal models and utilizing isolated human arteries to study vascular remodeling after injury...
August 10, 2018: Cell Transplantation
Modinat Liadi, Andrew Collins, Ying Li, Daqing Li
Trauma causes spinal cord injury, and the devastating consequences of the injury are due to the failure of the damaged central nervous system (CNS) axons to regenerate. Previous studies have shown that olfactory ensheathing cells (OECs) are a unique type of glial cell and they can promote regeneration of CNS axons to aid recovery after spinal cord injury. Transplantation of OECs, in particular from the olfactory bulb (OB), is considered one of the most promising therapeutic strategies for the repair of CNS injuries, including spinal cord injury...
August 10, 2018: Cell Transplantation
Fu Yuan Yang, Rui Chen, Xiaohu Zhang, Biao Huang, Lai Ling Tsang, Xican Li, Xiaohua Jiang
Mesenchymal stem cell (MSC)-based cell therapy has been demonstrated as a promising strategy in the treatment of inflammatory bowel disease (IBD), which is considered an immune disease. While the exact mechanisms underlying the therapeutic effect of MSCs are still unclear, MSCs display anti-inflammatory and immunomodulatory effects by interacting with various immunoregulatory cells. Our previous studies have shown that MSCs can be preconditioned and deconditioned with enhanced cell survival, differentiation and migration...
August 10, 2018: Cell Transplantation
Dan Wang, Yue-Qi Sun, Wen-Xiang Gao, Xing-Liang Fan, Jian-Bo Shi, Qing-Ling Fu
Induced pluripotent stem cell-derived mesenchymal stem cells (iPSC-MSCs) represent a promising cell source for patient-specific cell therapy. We previously demonstrated that they display an immunomodulatory effect on allergic airway inflammation. Glucocorticoids are powerful anti-inflammatory compounds and widely used in the therapy of allergic diseases. However, the effect of glucocorticoids on the immunomodulatory function of iPSC-MSCs remains unknown. This study aimed to determine the effect of dexamethasone (Dex) on the immunomodulatory function of iPSC-MSCs in vitro and in vivo...
July 30, 2018: Cell Transplantation
Jesper Dyrendom Svalgaard, Mehrnaz Safaee Talkhoncheh, Eva Kannik Haastrup, Lea Munthe-Fog, Christian Clausen, Morten Bagge Hansen, Pernille Andersen, Jette Sønderskov Gørløv, Jonas Larsson, Anne Fischer-Nielsen
Hematopoietic stem cell transplantation often involves the cryopreservation of stem cell products. Currently, the standard cryoprotective agent (CPA) is dimethyl sulfoxide (DMSO), which is known to cause concentration-related toxicity and side effects when administered to patients. Based on promising in vitro data from our previous study using pentaisomaltose (a 1 kDa subfraction of Dextran 1) as an alternative to DMSO for cryopreservation of hematopoietic progenitor cells (HPCs) from apheresis products, we proceeded to a preclinical model and compared the two CPAs with respect to engraftment of human hematopoietic stem and progenitor cells (HSPCs) in the immunodeficient NSG mouse model...
July 30, 2018: Cell Transplantation
Ming-Zhe Wu, Wei-Nan Li, Na Cha, Li-Xiang Tian, Y I Zhang, Xin Wu, Ke-Jun Guo, Guang-Ping Wu
Current human papillomavirus (HPV)16 DNA testing has high sensitivity but low specificity, while mRNA testing (qualitative) improves the specificity. However, both techniques are not able to discriminate between transient and persistent infections. To overcome the disadvantages, we quantitatively detected E6 and E7 mRNAs by quantitative real-time polymerase chain reaction (qRT-PCR) in cervical brushing cells from 87 HPV16+ and 31 HPV16- patients. Our results showed that the expression levels of E6 mRNA or E7 mRNA were significantly increased in HPV16-positive cases than that in the negative cases...
July 30, 2018: Cell Transplantation
Ao Feng, Chen Ling, Lin Xin-Duo, Wu Bing, Wu San-Wu, Zhan Yu, Huang Yu-Lan, Zhang You-En
Autophagy, an intracellular bulk degradation process of proteins and organelles, can be induced by myocardial ischemia in the heart. However, the causative role of autophagy in the survival of human cardiac fibroblasts and the underlying mechanisms are incompletely understood. Oxidative stress can induce autophagy in cultured cells upon hydrogen peroxide (H2 O2 ) exposure. Because hydrogen sulfide (H2 S) regulates reactive oxygen species (ROS) and apoptosis, we hypothesize that H2 S may have a cardioprotective function...
August 2018: Cell Transplantation
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