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Cell Transplantation

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https://www.readbyqxmd.com/read/28901195/feedforward-coordinate-control-of-a-robotic-cell-injection-catheter
#1
Weyland Cheng, Peter K Law
Remote and robotically actuated catheters are the stepping-stones toward autonomous catheters, where complex intravascular procedures may be performed with minimal intervention from a physician. This article proposes a concept for the positional, feedforward control of a robotically actuated cell injection catheter used for the injection of myogenic or undifferentiated stem cells into the myocardial infarct boundary zones of the left ventricle. The prototype for the catheter system was built upon a needle-based catheter with a single degree of deflection, a 3-D printed handle combined with actuators, and the Arduino microcontroller platform...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28901194/acute-renal-graft-versus-host-disease-in-a-murine-model-of-allogeneic-bone-marrow-transplantation
#2
Peter M Schmid, Abdellatif Bouazzaoui, Karin Schmid, Christoph Birner, Christian Schach, Lars S Maier, Ernst Holler, Dierk H Endemann
Acute kidney injury (AKI) is a very common complication after allogeneic bone marrow transplantation (BMT) and is associated with a poor prognosis. Generally, the kidneys are assumed to not be no direct targets of graft-versus-host disease (GvHD), and renal impairment is often attributed to several other factors occurring in the early phase after BMT. Our study aimed to prove the existence of renal GvHD in a fully major histocompatibility complex (MHC)-mismatched model of BALB/c mice conditioned and transplanted according to 2 different intensity protocols...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28901193/erk-and-p38-upregulation-versus-bcl-6-downregulation-in-rat-kidney-epithelial-cells-exposed-to-prolonged-hypoxia
#3
Fengbao Luo, Jian Shi, Qianqian Shi, Xiaozhou He, Ying Xia
Hypoxia is a common cause of kidney injury and a major issue in kidney transplantation. Mitogen-activated protein kinases (MAPKs) are involved in the cellular response to hypoxia, but the precise roles of MAPKs in renal cell reactions to hypoxic stress are not well known yet. This work was conducted to investigate the regulation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) and p38 and their signaling-relevant molecules in kidney epithelial cells exposed to prolonged hypoxia. Rat kidney epithelial cells Normal Rat Kidney (NRK)-52E were exposed to hypoxic conditions (1% O2) for 24 to 72 h...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28901192/neuronal-cell-sheets-of-cortical-motor-neuron-phenotype-derived-from-human-ipscs
#4
Noboru Suzuki, Nagisa Arimitsu, Jun Shimizu, Kenji Takai, Chieko Hirotsu, Yuji Ueda, Sueshige Wakisaka, Naruyoshi Fujiwara, Tomoko Suzuki
Transplantation of stem cells that differentiate into more mature neural cells brings about functional improvement in preclinical studies of stroke. Previous transplant approaches in the diseased brain utilized injection of the cells in a cell suspension. In addition, neural stem cells were preferentially used for grafting. However, these cells had no specific relationship to the damaged tissue of stroke and brain injury patients. The injection of cells in a suspension destroyed the cell-cell interactions that are suggested to be important for promoting functional integrity of cortical motor neurons...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28901191/hypoxic-culture-promotes-dopaminergic-neuronal-differentiation-of-nasal-olfactory-mucosa-mesenchymal-stem-cells-via-upregulation-of-hypoxia-inducible-factor-1%C3%AE
#5
Yi Zhuo, Lei Wang, Lite Ge, Xuan Li, Da Duan, Xiaohua Teng, Miao Jiang, Kai Liu, Ting Yuan, Pei Wu, Hao Wang, Yujia Deng, Huali Xie, Ping Chen, Ying Xia, Ming Lu
Olfactory mucosa mesenchymal stem cells (OM-MSCs) display significant clonogenic activity and may be easily propagated for Parkinson's disease therapies. Methods of inducing OM-MSCs to differentiate into dopaminergic (DAergic) neurons using olfactory ensheathing cells (OECs) are thus an attractive topic of research. We designed a hypoxic induction protocol to generate DAergic neurons from OM-MSCs using a physiological oxygen (O2) level of 3% and OEC-conditioned medium (OCM; HI group). The normal induction (NI) group was cultured in O2 at ambient air level (21%)...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28901190/transcriptome-analysis-of-induced-pluripotent-stem-cell-ipsc-derived-pancreatic-%C3%AE-like-cell-differentiation
#6
Yan Huang, Jian Wan, Yibing Guo, Shajun Zhu, Yao Wang, Lei Wang, Qingsong Guo, Yuhua Lu, Zhiwei Wang
Diabetes affects millions of people worldwide, and β-cell replacement is one of the promising new strategies for treatment. Induced pluripotent stem cells (iPSCs) can differentiate into any cell type, including pancreatic β cells, providing a potential treatment for diabetes. However, the molecular mechanisms underlying the differentiation of iPSC-derived β cells have not yet been fully elucidated. Here, we generated pancreatic β-like cells from mouse iPSCs using a 3-step protocol and performed deep RNA sequencing to get a transcriptional landscape of iPSC-derived pancreatic β-like cells during the selective differentiation period...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28901189/cryopreservation-of-hepatocyte-microbeads-for-clinical-transplantation
#7
Suttiruk Jitraruch, Anil Dhawan, Robin D Hughes, Celine Filippi, Sharon C Lehec, Leanne Glover, Ragai R Mitry
Intraperitoneal transplantation of hepatocyte microbeads is an attractive option for the management of acute liver failure. Encapsulation of hepatocytes in alginate microbeads supports their function and prevents immune attack of the cells. Establishment of banked cryopreserved hepatocyte microbeads is important for emergency use. The aim of this study was to develop an optimized protocol for cryopreservation of hepatocyte microbeads for clinical transplantation using modified freezing solutions. Four freezing solutions with potential for clinical application were investigated...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28901188/engineered-microvasculature-in-pdms-networks-using-endothelial-cells-derived-from-human-induced-pluripotent-stem-cells
#8
Amogh Sivarapatna, Mahboobe Ghaedi, Yang Xiao, Edward Han, Binod Aryal, Jing Zhou, Carlos Fernandez-Hernando, Yibing Qyang, Karen K Hirschi, Laura E Niklason
In this study, we used a polydimethylsiloxane (PDMS)-based platform for the generation of intact, perfusion-competent microvascular networks in vitro. COMSOL Multiphysics, a finite-element analysis and simulation software package, was used to obtain simulated velocity, pressure, and shear stress profiles. Transgene-free human induced pluripotent stem cells (hiPSCs) were differentiated into partially arterialized endothelial cells (hiPSC-ECs) in 5 d under completely chemically defined conditions, using the small molecule glycogen synthase kinase 3β inhibitor CHIR99021 and were thoroughly characterized for functionality and arterial-like marker expression...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28901187/peptide-modified-chitosan-hydrogels-accelerate-skin-wound-healing-by-promoting-fibroblast-proliferation-migration-and-secretion
#9
Xionglin Chen, Min Zhang, Shixuan Chen, Xueer Wang, Zhihui Tian, Yinghua Chen, Pengcheng Xu, Lei Zhang, Lu Zhang, Lin Zhang
Skin wound healing is a complicated process that involves a variety of cells and cytokines. Fibroblasts play an important role in this process and participate in transformation into myofibroblasts, the synthesis of extracellular matrix (ECM) and fibers, and the secretion of a variety of growth factors. This study assessed the effects of peptide Ser-Ile-Lys-Val-Ala-Val (SIKVAV)--modified chitosan hydrogels on skin wound healing. We investigated the capability of peptide SIKVAV to promote cell proliferation and migration, the synthesis of collagen, and the secretion of a variety of growth factors using fibroblasts in vitro...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28901186/recipient-adipose-derived-stem-cells-enhance-recipient-cell-engraftment-and-prolong-allotransplant-survival-in-a-miniature-swine-hind-limb-model
#10
Yur-Ren Kuo, Chien-Chang Chen, Yen-Chou Chen, Ching-Ming Chien
Donor mesenchymal stem cells (MSCs) could prolong vascularized composite allotransplantation (VCA) survival in our previous studies. However, recipient adipose tissue is easier to harvest than donor tissue for preconditioning modulation. Hence, this study investigated the efficacy of recipient autologous adipose-derived stem cells (rADSCs) for VCA survival. The heterotopic hind-limb transplantation from female donor to male recipient was performed in outbred miniature swine. Group I ( n = 6) was untreated controls...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28901185/evaluation-of-parametric-response-mapping-to-assess-therapeutic-response-to-human-mesenchymal-stem-cells-after-experimental-stroke
#11
Rui He, Anaïck Moisan, Olivier Detante, Chantal Rémy, Alexandre Krainik, Emmanuel Luc Barbier, Benjamin Lemasson
Stroke is the leading cause of disability in adults. After the very narrow time frame during which treatment by thrombolysis and mechanical thrombectomy is possible, cell therapy has huge potential for enhancing stroke recovery. Accurate analysis of the response to new therapy using imaging biomarkers is needed to assess therapeutic efficacy. The aim of this study was to compare 2 analysis techniques: the parametric response map (PRM), a voxel-based technique, and the standard whole-lesion approach. These 2 analyses were performed on data collected at 4 time points in a transient middle cerebral artery occlusion (MCAo) model, which was treated with human mesenchymal stem cells (hMSCs)...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28901184/ex-vivo-pretreatment-of-islets-with-mitomycin-c-reduction-in-immunogenic-potential-of-islets-by-suppressing-secretion-of-multiple-chemotactic-factors
#12
Naoya Sato, Junichiro Haga, Takayuki Anazawa, Akira Kenjo, Takashi Kimura, Ikuo Wada, Tsutomu Mori, Shigeru Marubashi, Mitsukazu Gotoh
Strategies to reduce the immunogenicity of pancreatic islets and to prevent the activation of proinflammatory events are essential for successful islet engraftment. Pretransplant islet culture presents an opportunity for preconditioning to improve outcomes of islet transplantation. We previously demonstrated that ex vivo mitomycin C (MMC) pretreatment and subsequent culture significantly prolonged graft survival. Fully understanding the biological process of pretreatment could result in the development of a protocol to improve the survival of islet grafts...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28901183/ppar-%C3%AE-agonist-with-mesenchymal-stem-cells-induces-type-ii-collagen-producing-chondrocytes-in-human-arthritic-synovial-fluid
#13
Bruce E Heck, Joshua J Park, Vishruti Makani, Eun-Cheol Kim, Dong Hyun Kim
Osteoarthritis (OA) is an inflammatory joint disease characterized by degeneration of articular cartilage within synovial joints. An estimated 27 million Americans suffer from OA, and the population is expected to reach 67 million in the United States by 2030. Thus, it is urgent to find an effective treatment for OA. Traditional OA treatments have no disease-modifying effect, while regenerative OA therapies such as autologous chondrocyte implantation show some promise. Nonetheless, current regenerative therapies do not overcome synovial inflammation that suppresses the differentiation of mesenchymal stem cells (MSCs) to chondrocytes and the expression of type II collagen, the major constituent of functional cartilage...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28901182/effects-of-bone-marrow-derived-msc-transplantation-on-functional-recovery-in-a-rat-model-of-spinal-cord-injury-comparisons-of-transplant-locations-and-cell-concentrations
#14
Jessica J Matyas, Andrew N Stewart, Alison Goldsmith, Zhenhong Nan, Reid L Skeel, Julien Rossignol, Gary L Dunbar
Spinal cord injury (SCI) is a widely disabling condition, constraining those affected by it to wheelchairs and requiring intense daily care and assistance. Cell replacement therapies, targeting regeneration of cells in the injured cord, are currently gaining momentum in the field of SCI research. Previous studies indicate that mesenchymal stem cells (MSCs) can reduce functional deficits through immunomodulation and production of trophic factors in a variety of neurological disorders. The present study assessed the efficacy of transplanted bone marrow-derived MSCs at different concentrations and locations for promoting functional recovery following SCI...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28185609/olfactory-ensheathing-cells-inhibit-gliosis-in-retinal-degeneration-by-downregulation-of-the-m%C3%A3-ller-cell-notch-signaling-pathway
#15
Jing Xie, Shujia Huo, Yijian Li, Jiaman Dai, Haiwei Xu, Zheng Qin Yin
Retinal regeneration and self-repair, whether in response to injury or degenerative disease, are severely impeded by glial scar formation by Müller cells (specialized retinal macroglia). We have previously demonstrated that the activation of Müller cells and gliosis in the degenerative retina are significantly suppressed by the subretinal transplantation of a mixture of olfactory ensheathing cells (OECs) and olfactory nerve fibroblasts. However, the underlying molecular mechanism has remained elusive. Here we transplanted purified rat OECs into the subretinal space of pigmented Royal College of Surgeons (RCS) rats, a classic rodent model of retinal degeneration...
June 9, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28160460/the-timing-of-immunomodulation-induced-by-mesenchymal-stromal-cells-determines-the-outcome-of-the-graft-in-experimental-renal-allotransplantation
#16
Ana Merino, Elia Ripoll, Laura de Ramon, Nuria Bolaños, Montserrat Goma, Oriol Bestard, Nuria Lloberas, Josep M Grinyo, Juan Torras Ambròs
The immunomodulatory characteristics of mesenchymal stromal cells (MSCs) may lead to multifaceted strategies in rejection of organ transplantation. This study was designed to investigate, first, the effect of the donor-type MSCs from Wistar rats on the immune system of immunocompetent Lewis rats and, second, the rejection responses in a renal transplantation model of Wistar to Lewis. In the first experimental model, MSCs from the bone marrow induced a systemic immune response in the immunocompetent Lewis rats, characterized by two different phases...
June 9, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28155815/pig-to-primate-islet-xenotransplantation-past-present-and-future
#17
Zhengzhao Liu, Wenbao Hu, Tian He, Yifan Dai, Hidetaka Hara, Rita Bottino, David K C Cooper, Zhiming Cai, Lisha Mou
Islet allotransplantation results in increasing success in treating type 1 diabetes, but the shortage of deceased human donor pancreata limits progress. Islet xenotransplantation, using pigs as a source of islets, is a promising approach to overcome this limitation. The greatest obstacle is the primate immune/inflammatory response to the porcine (pig) islets, which may take the form of rapid early graft rejection (the instant blood-mediated inflammatory reaction) or T-cell-mediated rejection. These problems are being resolved by the genetic engineering of the source pigs combined with improved immunosuppressive therapy...
June 9, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28155812/human-muscle-precursor-cells-overexpressing-pgc-1%C3%AE-enhance-early-skeletal-muscle-tissue-formation
#18
Deana Haralampieva, Souzan Salemi, Ivana Dinulovic, Tullio Sulser, Simon M Ametamey, Christoph Handschin, Daniel Eberli
Muscle precursor cells (MPCs) are activated satellite cells capable of muscle fiber reconstruction. Therefore, autologous MPC transplantation is envisioned for the treatment of muscle diseases. However, the density of MPCs, as well as their proliferation and differentiation potential, gradually declines with age. The goals of this research were to genetically modify human MPCs (hMPCs) to overexpress the peroxisome proliferator-activated receptor γ coactivator (PGC-1α), a key regulator of exercise-mediated adaptation, and thereby to enhance early skeletal muscle formation and quality...
June 9, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28155808/using-electrical-stimulation-to-enhance-the-efficacy-of-cell-transplantation-therapies-for-neurodegenerative-retinal-diseases-concepts-challenges-and-future-perspectives
#19
Abby Leigh Manthey, Wei Liu, Zhi Xin Jiang, Marcus Hiu Kong Lee, Jian Ji, Kwok-Fai So, Jimmy Shiu Ming Lai, Vincent Wing Hong Lee, Kin Chiu
Disease or trauma-induced loss or dysfunction of neurons in any central nervous system (CNS) tissue will have a significant impact on the health of the affected patient. The retina is a multilayered tissue that originates from the neuroectoderm, much like the brain and spinal cord. While sight is not required for life, neurodegeneration-related loss of vision not only affects the quality of life for the patient but also has societal implications in terms of health care expenditure. Thus, it is essential to develop effective strategies to repair the retina and prevent disease symptoms...
June 9, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28155807/cisplatin-impaired-adipogenic-differentiation-of-adipose-mesenchymal-stem-cells-1
#20
Yu-Hsun Chang, Hwan-Wun Liu, Tang-Yuan Chu, Yao-Tseng Wen, Rong-Kung Tsai, Dah-Ching Ding
Adipose tissue-derived mesenchymal stem cells (ADSCs) are derived from adipose tissue and can be induced in vitro to differentiate into osteoblasts, chondroblasts, myocytes, neurons, and other cell types. Cisplatin is a commonly used chemotherapy drug for cancer patients. However, the effects of cisplatin on ADSCs remain elusive. This study found that a high concentration of cisplatin affects the viability of ADSCs. First, the IC50 concentration of cisplatin was evaluated. Proliferation of ADSCs, as assessed by the XTT method, decreased immediately after treatment with various concentrations of cisplatin...
June 9, 2017: Cell Transplantation
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