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Cell Transplantation

Yohei Tanikake, Manabu Akahane, Akira Furukawa, Yasuaki Tohma, Yusuke Inagaki, Tsutomu Kira, Yasuhito Tanaka
Artificial bones made of β-tricalcium phosphate (β-TCP) combined with bone marrowderived stromal cells (BMSCs) are used for effective reconstruction of bone defects caused by genetic defects, traumatic injury, or surgical resection of bone tumors. However, the selection of constructs with high osteogenic potential before implantation is challenging. The purpose of this study was to determine whether the calcium concentration in BMSC culture medium can be used as a nondestructive and simple osteogenic marker for selecting tissueengineered grafts constructed using β-TCP and BMSCs...
November 29, 2016: Cell Transplantation
Shiying Li, Xiaosong Gu, Sheng Yi
The transforming growth factor beta (TGF-β) belongs to a group of pleiotropic cytokines that are involved in avariety of biological processes, such as inflammation and immune reaction, cellular phenotype transition, extracellular matrix (ECM) disposition and epithelial-mesenchymal transition. TGF-β is widely distributed throughout the body, including the nervous system. Following injury to the nervous system, TGF-β regulates the behavior of neurons and glial cells and thus mediates the regenerative process...
November 23, 2016: Cell Transplantation
Nizar Mourad, Andrea Perota, Daela Xhema, Cesare Galli, Pierre Gianello
Porcine islets have notoriously low insulin secretion levels in response to glucose stimulation. While this is somehowexpected in the case of immature islets isolated from fetal and neonatal pigs, disappointingly low secretory responses are frequently reported in studies using <em>in vitro</em>-maturated fetal and neonatal islets and even fully-differentiated adult islets. Herein, we show that beta-cell specific expression of a modified glucagon-like peptide 1 (GLP-1) and of a constitutively activated type 3 muscarinic receptor (M3R) efficiently amplifies glucose-stimulated insulin secretion (GSIS)...
November 22, 2016: Cell Transplantation
Xiaojing Lin, Tingbao Zhao, Melissa Walker, Aishi Ding, Shide Lin, Yongcheng Cao, Jinfeng Zheng, Xiaohong Liu, Ming Geng, Xiao-Ming Xu, Shaojun Liu
Spinal cord injury (SCI) is a significant clinical challenge, and to date no effective treatment is available. Oligodendrocyte progenitor cell (OPC) transplantation has been a promising strategy for SCI repair. However, the poor posttransplantation survival and deficiency in differentiation into myelinating oligodendrocytes (OLs) are two major challenges that limit the use of OPCs as donor cells. Here we report the generation of an OL lineage population [i.e., pro-oligodendroblasts (proOLs)] that is relatively more mature than OPCs for transplantation after SCI...
December 13, 2016: Cell Transplantation
Kazuyoshi Kanki, Masaaki Ii, Yoshito Terai, Masahide Ohmichi, Michio Asahi
Bone marrow-derived endothelial progenitor cells (EPCs) have been shown to contribute to not only angiogenesis in ischemic tissue but also neovascularization in uterine endometrium formation. Reduced neovascularization and elevation of serum soluble Flt1, a functional blockage of VEGF, in the development of placenta is thought to be one of the major causes of repeated miscarriages in gestation. We then examined whether transfusion of VEGF-expressing extrinsic EPCs prevented frequent miscarriage via its promotional effect on neovascularization with a VEGFeNOS signaling pathway in a mouse miscarriage model...
December 13, 2016: Cell Transplantation
Vanessa Sauer, Tatyana Tchaikovskaya, Xia Wang, Yanfeng Li, Wei Zhang, Krisztina Tar, Zsuzsanna Polgar, Jianqiang Ding, Chandan Guha, Ira J Fox, Namita Roy-Chowdhury, Jayanta Roy-Chowdhury
Although several types of somatic cells have been reprogrammed into induced pluripotent stem cells (iPSCs) and then differentiated to hepatocyte-like cells (iHeps), the method for generating such cells from renal tubular epithelial cells shed in human urine and transplanting them into animal livers has not been described systematically. We report reprogramming of human urinary epithelial cells into iPSCs and subsequent hepatic differentiation, followed by a detailed characterization of the newly generated iHeps...
December 13, 2016: Cell Transplantation
Bin He, Qianmin Yao, Zijing Liang, Junmin Lin, Yubao Xie, Shaoming Li, Gongxiong Wu, Zhihua Yang, Pingyi Xu
The therapeutic benefits of bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation for ischemic stroke have been extensively demonstrated. However, studies on the optimal cell dose for intravenous administration are still limited. This study aimed to determine an appropriate cell dose for BM-MSC intravenous transplantation and to investigate the effect of cell dose on vascular remodeling in a rat model of ischemic stroke. BM-MSCs at doses of 5104 (low-dose group), 5105 (medium-dose group), and 2106 (high-dose group) were intravenously injected into rats at 72 h after ischemia...
December 13, 2016: Cell Transplantation
Luciana B SantAnna, Raduan Hage, Maria Anglica G Cardoso, Emilia A L Arisawa, Maria Martin Cruz, Ornella Parolini, Anna Cargnoni, Nilson SantAnna
Liver fibrosis is characterized by excessive accumulation of extracellular matrix components in the liver parenchyma that distorts the normal architecture and hepatic function. Progressive fibrosis could end in the advanced stage known as cirrhosis, resulting in the need to resort to liver transplantation. Amniotic membrane (AM) has emerged as an innovative therapeutic approach for chronic liver diseases due to its anti-inflammatory, antiscarring, and wound-healing effects. We have recently shown that AM can be used as a patch on the liver surface at the same time of fibrosis induction, resulting in significantly reduced progression and severity of biliary fibrosis...
December 13, 2016: Cell Transplantation
Deepak Kumar, Alex Lyness, Irini Gerges, Christina Lenardi, Nicholas R Forsyth, Yang Liu
Nucleus pulposus (NP) tissue damage can induce detrimental mechanical strain on the biomechanical performance of intervertebral discs (IVDs), causing subsequent disc degeneration. A novel, photocurable, injectable, synthetic polymer hydrogel (pHEMA-co-APMA grafted with PAA) has already demonstrated success in encapsulating and differentiating human mesenchymal stem cells (hMSCs) toward an NP phenotype during hypoxic conditions. After demonstration of promising results in our previous work, in this study we have further investigated the inclusion of mechanical stimulation and its impact on hMSC differentiation toward an NP phenotype through the characterization of matrix markers such as SOX-9, aggrecan, and collagen II...
December 13, 2016: Cell Transplantation
Ji Hyeon Ahn, Bai Hui Chen, Bich Na Shin, Jeong Hwi Cho, In Hye Kim, Joon Ha Park, Jae Chul Lee, Hyun Jin Tae, Yun Lyul Lee, Jaesuk Lee, Kyunghee Byun, Goo-Bo Jeong, Bonghee Lee, Seung U Kim, Young-Myeong Kim, Moo-Ho Won, Soo Young Choi
Oligodendrocytes play a crucial role in creating the myelin sheath that is an important component in neural transmission. In an animal model of transient cerebral ischemia, application of oligodendrocyte progenitor cells (OPCs) has not yet been reported. In this study, the effects of F3.Olig2 transplantation on memory and cognitive dysfunction were investigated in the aged gerbil in which ischemic stroke was induced. To investigate the possible mechanisms underlying repair, changes in the expression of myelin basic protein (MBP), oligodendrocyte-specific protein (OSP), and brain-derived neurotrophic factor (BDNF) were examined...
December 13, 2016: Cell Transplantation
Janina Burk, Dagmar Berner, Walter Brehm, Aline Hillmann, Carolin Horstmeier, Christoph Josten, Felicitas Paebst, Giacomo Rossi, Susanna Schubert, Annette B Ahrberg
Tendon disease has been treated with multipotent mesenchymal stromal cells (MSCs) in the equine large-animal model with promising success. The aim of this study was to gain more insight into the fate and biodistribution of MSCs after local application into tendon lesions by long-term cell tracking in this large-animal model. Superficial digital flexor tendon lesions were induced in all limbs in six horses and injected with 10106 Molday ION Rhodamine B-labeled MSCs suspended in serum or serum alone. Follow-up was performed using low-field magnetic resonance imaging (MRI), flow cytometry, and histology...
December 13, 2016: Cell Transplantation
Alberto Martnez-Serrano, Marta P Pereira, Natalia Avaliani, Anna Nelke, Merab Kokaia, Tania Ramos-Moreno
Cell replacement therapy in Parkinsons disease (PD) still lacks a study addressing the acquisition of electrophysiological properties of human grafted neural stem cells and their relation with the emergence of behavioral recovery after transplantation in the short term. Here we study the electrophysiological and biochemical profiles of two ventral mesencephalic human neural stem cell (NSC) clonal lines (C30-Bcl-XL and C32-Bcl-XL) that express high levels of Bcl-XL to enhance their neurogenic capacity, after grafting in an in vitro parkinsonian model...
December 13, 2016: Cell Transplantation
David Habart, Jan Vihlk, Jan Schier, Monika Cahov, Peter Girman, Klra Zacharovov, Zuzana Berkov, Jan K, Eva Fabryov, Lucie Kosinov, Zuzana Papkov, Jan Kybic, Frantiek Saudek
Clinical islet transplantation programs rely on the capacities of individual centers to quantify isolated islets. Current computer-assisted methods require input from human operators. Here we describe two machine learning algorithms for islet quantification: the trainable islet algorithm (TIA) and the nontrainable purity algorithm (NPA). These algorithms automatically segment pancreatic islets and exocrine tissue on microscopic images in order to count individual islets and calculate islet volume and purity...
December 13, 2016: Cell Transplantation
Vaihere Delaune, Christian Toso, Pierre-Yves Benhamou, Anne Wojtusciszyn, Laurence Kessler, Florence Slits, Sandrine Demuylder-Mischler, Nadine Pernin, Sandrine Lablanche, Lorenzo A Orci, Graziano Oldani, Philippe Morel, Thierry Berney, Stphanie Lacotte
Islet transplantation is an effective treatment for selected patients with type 1 diabetes. However, an accurate test still lacks for the early detection of graft rejection. Blood samples were prospectively collected in four university centers (Geneva, Grenoble, Montpellier, and Strasbourg). Peripheral blood mononuclear cells were stimulated with donor splenocytes in the presence of interleukin-2. After 24 h of incubation, interferon- (IFN-) ELISpot analysis was performed. After a total of 5 days of incubation, cell proliferation was assessed by fluorescence-activated cell sorting (FACS) analysis for Ki-67...
December 13, 2016: Cell Transplantation
Magnus Sthle, Aksel Foss, Bengt Gustafsson, Marko Lempinen, Torbjrn Lundgren, Ehab Rafael, Gunnar Tufveson, Bastian Theisinger, Daniel Brandhorst, Olle Korsgren, Andrew Friberg
This study aimed to evaluate a 50:50 mix of perfluorohexyloctane/polydimethylsiloxane 5 (F6H8S5) preservation of pancreases in a clinical setting compared with standard solutions for 1) cold ischemia time (CIT) 10 h and 2) an extended CIT 20 h. Procured clinical-grade pancreases were shipped in either F6H8S5 or in standard preservation solutions, that is, University of Wisconsin (UW) or Custodiol. F6H5S5 was preoxygenated for at least 15 min. Included clinical-grade pancreases were procured in UW or Custodiol...
December 13, 2016: Cell Transplantation
Xiang Zeng, Inbo Han, Muhammad Abd-El-Barr, Zaid Aljuboori, Jamie E Anderson, John H Chi, Ross D Zafonte, Yang D Teng
The adult rodent spinal cord presents an inhibitory environment for donor cell survival, impeding efficiency for xenograft-based modeling of gliomas. We postulated that mild thermal preconditioning may influence the fate of the implanted tumor cells. To test this hypothesis, high-grade human astrocytoma G55 and U87 cells were cultured under 37C and 38.5C to mimic regular experimental or core body temperatures of rodents, respectively. In vitro, the 38.5C-conditioned cells, relative to 37C, grew slightly faster...
December 13, 2016: Cell Transplantation
Anack Moisan, Isabelle Favre, Claire Rome, Florence De Fraipont, Emmanuelle Grillon, Nicolas Coquery, Herv Mathieu, Virginie Mayan, Bernadette Naegele, Marc Hommel, Marie-Jeanne Richard, Emmanuel Luc Barbier, Chantal Remy, Olivier Detante
Stroke is the leading cause of disability in adults. Many current clinical trials use intravenous (IV) administration of human bone marrow-derived mesenchymal stem cells (BM-MSCs). This autologous graft requires a delay for ex vivo expansion of cells. We followed microvascular effects and mechanisms of action involved after an IV injection of human BM-MSCs (hBM-MSCs) at a subacute phase of stroke. Rats underwent a transient middle cerebral artery occlusion (MCAo) or a surgery without occlusion (sham) at day 0 (D0)...
December 13, 2016: Cell Transplantation
Huishan Zhang, Huashan Zhao, Jingqiao Qiao, Shoubing Zhang, Shuang Liu, Na Li, Xiaohua Lei, Lina Ning, Yujing Cao, Enkui Duan
Hair follicle stem cells (HFSCs) are considered one of the useful donor cell types for skin regenerative medicine owing to their robust proliferative capacity and multipotency. However, methods for easily and effectively obtaining HFSCs from a limited skin biopsy are still lacking. Here we report a novel approach for obtaining a subpopulation of HFSCs from a small skin sample from the rat tail, which uses the sebaceous glands (SGs) to capture the adjacent HFSCs. By means of organ culture, keratinocytes were expanded from the detached SGs, which also included adherent HFSCs from the hair follicle that could be passaged at the single-cell level...
November 2016: Cell Transplantation
Miguel M De Jesus, Jayson S Santiago, Camille V Trinidad, Melvin E See, Kimberly R Semon, Manuel O Fernandez, Francisco S Chung
Psoriasis is a dermatologic disease of immune origins with no definitive cure. We report the Makati Medical Center experience of utilizing autologous mesenchymal stromal cells (MSCs) for one patient with psoriasis vulgaris (PV) and another with psoriatic arthritis (PA). Patients were educated and gave informed consent, according to the principles of the Declaration of Helsinki. The protocol was approved by the Cellular Transplantation Ethics Committee of the Makati Medical Center. Autologous MSCs were cultured from lipoaspirate and expanded in a clean room class 100 facility (Cellular Therapeutics Center, Makati Medical Center)...
November 2016: Cell Transplantation
Peter M Schmid, Abdellatif Bouazzaoui, Karin Schmid, Christoph M Birner, Christian Schach, Lars S Maier, Ernst Holler, Dierk H Endemann
Graft-versus-host disease (GVHD) is the limiting complication after bone marrow transplantation (BMT), and its pathophysiology seems to be highly influenced by vascular factors. Our study aimed at elucidating possible mechanisms involved in vascular GVHD. For this purpose, we used a fully MHC-mismatched model of BALB/c mice conditioned according to two different intensity protocols with total body irradiation and transplantation of allogeneic (C57BL/6) or syngeneic bone marrow cells and splenocytes. Mesenteric resistance arteries were studied in a pressurized myograph...
November 2016: Cell Transplantation
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