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Transplantation Proceedings

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https://www.readbyqxmd.com/read/30177143/comparison-of-vascular-endothelial-growth-factor-concentration-in-porcine-kidneys-removed-from-living-donors-after-cardiac-and-brain-death
#1
G Oczkowicz, A Caban, G Budziński, A Suszka-Świtek, B Dolińska, E Czech, R Wiaderkiewicz, F Ryszka, L Cierpka, R Król
The aim of this paper was to describe the differences in vascular endothelial growth factor (VEGF) concentration in porcine kidneys removed from living donors (group I), donors after prior induction of brain death by brain herniation (group II), and donors after cardiopulmonary arrest (group III). The groups consisted of 6 animals which underwent dual renal removal procedures; kidneys were rinsed, stored for 24 hours at 4°C and rinsed again. Renal specimens (4g) were collected before and after perfusion (time 0 and 1), after 12 hours (time 2), and after reperfusion (time 3)...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177142/evaluation-of-the-relationship-between-concentrations-of-tacrolimus-metabolites-13-o-demethyl-tacrolimus-and-15-o-demethyl-tacrolimus-and-clinical-and-biochemical-parameters-in-kidney-transplant-recipients
#2
J Zegarska, E Hryniewiecka, D Zochowska, E Samborowska, R Jazwiec, K Maciej, S Nazarewski, M Dadlez, L Paczek
BACKGROUND: Tacrolimus (Tac), an essential component of immunosuppressive therapy after solid-organ transplantation, has a narrow therapeutic index and requires therapeutic drug monitoring. Monitoring of Tac predose blood concentrations seems to be not always sufficient to avoid adverse effects. The aim of the study was to evaluate the levels of main Tac metabolites, 13-O-demethyl tacrolimus (13-DMT), 31-O-demethyl tacrolimus (31-DMT), and 15-O-demethyl tacrolimus (15-DMT), in kidney transplant recipients and to link them to clinical and biochemical parameters...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177141/effect-of-prolactin-on-biochemical-and-morphological-parameters-of-rabbit-liver-in-warm-ischemia
#3
B Szulc-Musioł, B Dolińska, K Jasik, F Ryszka
BACKGROUND: The aim of the study was to assess the degree of liver damage in a rabbit perfused with histidine-tryptophan-ketoglutarate (HTK [Custodiol]) solution with and without the presence of prolactin (PRL) based on biochemical studies in perfundate and ultrastructural analysis of hepatocytes. MATERIALS AND METHODS: The experiment was carried out on rabbits. Liver ischemia was used in the study, based on Pringle's maneuver. About 70% of the rabbit liver lobes were perfused with HTK with or without the addition of PRL (2...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177140/in-vitro-and-in-vivo-rabbit-guinea-pig-mouse-properties-of-a-novel-resorbable-polymer-and-allogenic-bone-composite-for-guided-bone-regeneration-and-orthopedic-implants
#4
G Gut, M Ambroziak, W Bojar, B Szaraniec, J Chłopek, S Flis, M Koronkiewicz, Z Jastrzębski
INTRODUCTION: Currently, there is no bone fixation material that could be fully replaced by the competent recipient bone. The creeping substitution of the bone graft by the recipient bone is the result of its unique potential related to the presence of bone morphogenetic proteins (BMPs). However, the size of the human bone limits the use of allogenic implants for surgical (orthopedic) fixation. The aim of this project was to develop a novel composite material for guided bone regeneration, consisting of human bone powder obtained from a tissue bank and a resorbable polymer (13 wt% of bone powder in a medical poly-l-lactide polymer)...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177139/pericarditis-in-patients-with-chronic-graft-vs-host-disease
#5
E Karakulska-Prystupiuk, G Basak, J Dwilewicz-Trojaczek, M Paluszewska, P Boguradzki, W Jędrzejczak
BACKGROUND: There are only a few cases of pericarditis complications following allogeneic bone marrow transplantation described in the literature and there are no data available on the risk and frequency of this condition. The aim of this study was to assess the frequency of exudative pericarditis complicating chronic graft-vs-host disease in allogeneic hematopoietic cell transplant recipients. METHODS: Retrospective analysis involved a group of 105 patients of the Outpatient Transplantation Service of the Department of Hematology, Medical University of Warsaw, who received transplants in the years 2010-2016 and were evaluated for the years 2014-2016...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177138/azacitidine-for-relapse-after-allogeneic-stem-cell-transplantation-single-center-study
#6
E Karakulska-Prystupiuk, J Drozd-Sokołowska, A Waszczuk-Gajda, A Stefaniak, J Dwilewicz-Trojaczek, A Kulikowska, E Chmarzyńska-Mróz, G Basak, M Paluszewska, P Boguradzki, W Jędrzejczak
BACKGROUND: Relapse is the leading cause of treatment failure for myeloid malignancies treated with allogeneic hematopoietic stem cell transplantation. Treatment options are very limited and use of azacitidine is one of the available options. METHODS: This was a retrospective, single-institution study. Of 28 evaluated patients, 18 were males, and the median age was 60 years (range, 15-78). There were 15 patients with acute myeloid leukemia, 8 with myelodysplastic syndrome, 4 with chronic myelomonocytic leukemia, and 1 with primary myelofibrosis...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177137/influence-of-clonal-plasma-cell-contamination-of-peripheral-blood-stem-cell-autografts-on-progression-and-survival-in-multiple-myeloma-patients-after-autologous-peripheral-blood-stem-cell-transplantation-in-long-term-observation
#7
A Waszczuk-Gajda, M Feliksbrot-Bratosiewicz, M Król, E Snarski, J Drozd-Sokołowska, P Biecek, M Król, Z Lewandowski, J Peradzyńska, W W Jędrzejczak, J Dwilewicz-Trojaczek
BACKGROUND: High-dose chemotherapy followed by autologous peripheral blood stem cell transplantation (auto-PBSCT) remains the mainstay of treatment of eligible patients diagnosed multiple myeloma. The role of clonal plasma cell (CPC) contamination was found as a reason for relapse, but results in terms of survival, progression, and purging were ambiguous. Therefore, the aim of the study was to explore the influence of CPC contamination in the autograft on survival and progression after auto-PBSCT...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177136/evaluation-of-the-safety-and-clinical-efficacy-of-allogeneic-bone-grafts-in-the-reconstruction-of-the-maxilla-and-mandible
#8
Z Stopa, M Siewert-Gutowska, K Abed, D Szubińska-Lelonkiewicz, A Kamiński, P Fiedor
INTRODUCTION: Loss of teeth caused by inflammatory processes or trauma is one of the causes of bone atrophy of the maxilla alveolar process and the alveolar part of the mandible. Often, restoring these deficiencies with dental implants requires additional reconstructive procedures. Methods using autogenous, allogeneic, xenogeneic, or synthetic bone grafts are commonly used. MATERIALS AND METHODS: Patients who had bone atrophy of the maxilla or mandible were qualified for deep-frozen transplantation, radiation-sterilized allogeneic bone from the Bank of Tissues in the form of cortico-spongy bone blocks and spongy bone granules...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177135/influence-of-cryopreservation-on-structural-chemical-and-immunoenzymatic-properties-of-aortic-valve-allografts
#9
R Pfitzner, D Barecka, M Pawlikowski, M Kopytek, L Rudnicka-Sosin, E Majewska, M Mazur, Z Marcinkowska
OBJECTIVES: The problems in preparing (including cryopreservation) and implanting aortic valve allografts (AVAs) is widely elaborated, but some issues need explanation. MATERIAL AND METHODS: Twenty AVAs cryopreserved in dimethylsulphoxide/RPMI solution under -160°C for 1-15 years and 3 controls stored at +4°C up to 2 weeks, from 19 male and 4 female donors, aged 20-51, ±30.8 years, were examined using light (LM), digital (DM), and scanning electron microscopy (SEM), energy dispersion X-ray spectroscopy (EDS), and enzyme-linked immunosorbent assay immunoenzymatic tests (PECAM1, CD34)...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177134/experience-in-using-fetal-membranes-the-present-and-new-perspectives
#10
A Klama-Baryła, W Łabuś, D Kitala, M Kraut, M Nowak, M Kawecki
INTRODUCTION: The placenta is an accessible source of tissues for transplantation. Placental transplants have been used in wound treatment because of the basic function of the placenta and its nutritious properties and structure. PATIENTS AND METHODS: The aim of this work is to present the clinical usage of fetal membranes, including human amnion, on the basis of the burn treatment center's experience. The clinical use of amnion and different types of placental transplants are described...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177133/autologous-and-allogeneic-skin-cell-grafts-in-the-treatment-of-severely-burned-patients-retrospective-clinical-study
#11
A Klama-Baryła, D Kitala, W Łabuś, M Kraut, J Glik, M Nowak, M Kawecki
BACKGROUND: Transplantation of skin cells (keratinocytes and fibroblasts) cultured in vitro is a method of choice for the treatment of severe and extensive burns in patients with a deficit of donor sites for free split-thickness skin grafts, and when the grave medical condition of the patient excludes the possibility of an operation under general anesthetic. Appropriate amounts of keratinocytes and/or fibroblasts cultured in vitro are grafted as a suspension in platelet-leukocyte-rich gel directly on the prepared acceptor site...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177132/frequency-of-pathologic-changes-in-the-oral-cavity-in-patients-subjected-to-long-term-pharmacologic-immunosuppressive-therapy-after-kidney-liver-and-hematopoietic-cell-transplantation
#12
M Osiak, D Szubińska-Lelonkiewicz, P Wychowański, E Karakulska-Prystupiuk, W Jędrzejczak, A Wojtowicz, P Fiedor
INTRODUCTION: Patients subjected to long-term immunosuppressive therapy after organ and cells transplantation are more susceptible than healthy people to the development of the pathologic changes in the oral cavity, including precancerous lesions, oral cancers, lesions following viral infections (herpes simplex virus, Epstein-Barr virus, and cytomegalovirus), fungal infections mainly caused by Candida albicans, drug-induced gingival overgrowth, stomatitis, and tongue disorders. MATERIAL AND METHODS: Clinical case material included 38 patients after kidney, liver, or blood-forming cells transplantation subjected to various immunosuppressive therapy schemes...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177131/resistance-to-aminoglycosides-of-methicillin-resistant-strains-of-staphylococcus-aureus-originating-in-the-surgical-and-transplantation-wards-of-the-warsaw-clinical-center-a-retrospective-analysis
#13
K Szymanek-Majchrzak, A Mlynarczyk, D Kawecki, M Pacholczyk, M Durlik, D Deborska-Materkowska, L Paczek, G Mlynarczyk
INTRODUCTION: Aminoglycoside resistance (AR) is common in health care-associated methicillin-resistant Staphylococcus aureus (HA-MRSA). AR is most often associated with the production of antibiotic modifying enzymes: bidomain AAC(6')-Ie/APH(2″)-Ia acetyltransferase and phosphotransferase, ANT(4')-Ia nucleotidyltransferase, and APH(3″)-IIIa phosphotransferase. AIM: Determination of aminoglycoside sensitivity, presence of genes encoding enzymes, and molecular typing of HA-MRSA strains derived from patients hospitalized in surgical and transplantation wards...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177130/significance-of-screening-tests-and-the-incidence-of-new-delhi-metallo-beta-lactamase-producing-gram-negative-bacilli-in-the-surgery-and-transplantation-wards-of-a-warsaw-medical-center-during-the-period-from-april-2014-to-may-2017
#14
K Szymanek-Majchrzak, A Mlynarczyk, R Kuthan, A Sawicka-Grzelak, K Majchrzak, D Kawecki, M Kosieradzki, M Durlik, D Deborska-Materkowska, L Paczek, G Mlynarczyk
BACKGROUND: The first New Delhi metallo-beta-lactamase (NDM)-producing bacteria were isolated in 2008 in the world, and in 2011 in Poland. Due to the high clonal diversity (17 types) of their blaNDM gene, encoded on (Tn125-like) mobile genetic elements, these strains usually exhibit resistance to nearly all available antibiotics, which is particularly dangerous for organ transplant recipients. PURPOSE: To assess of the prevalence of Gram-negative NDM-positive bacilli in surgery/transplantation wards of a teaching hospital in Warsaw and to ascertain the significance of screening tests on the rates and nature of colonization...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177129/posttransplant-lymphoproliferative-disorder-in-kidney-and-liver-transplant-recipients-a-single-center-experience
#15
M Wójciak, J Gozdowska, D Dęborska-Materkowska, A Perkowska-Ptasińska, M Kosieradzki, S Nazarewski, M Durlik
INTRODUCTION: Posttransplant lymphoproliferative disorder (PTLD) is a heterogeneous group of lymphoid malignant neoplasms arising after solid organ transplantation or hematopoietic stem cell transplantation. The current World Health Organization classification identified 4 basic histologic types of PTLD: early, polymorphic variant, monomorphic variant, and classical Hodgkin lymphoma-type lesions. METHODS: Data of 12 PTLD cases of was retrospectively analyzed in terms of the transplanted organs, time to diagnosis of PTLD, type of immunosuppressive treatment in regard to the induction treatment and acute transplant rejection, and long-term survival...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177128/delivery-method-in-patients-after-liver-or-kidney-transplantation
#16
A Madej, N Mazanowska, M Szpotańska-Sikorska, B Kociszewska-Najman, D Warzecha, F Dąbrowski, Z Jabiry-Zieniewicz, A Cyganek, J Pazik, M Wielgoś, B Pietrzak
Pregnancy following renal or liver transplant is safe for the mother, fetus, and allograft if standard practice guidelines are strictly followed. Cesarean delivery is often required for the safety of the mother and child. The aim of this paper was the evaluation of delivery method in patients after liver (G1) and kidney transplantation (G2) in comparison with the population of healthy pregnant women (G0). MATERIALS: Retrospective analysis included 51 (G1) and 59 (G2) women who delivered between 2000 and 2016...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177127/parental-attitudes-and-medication-adherence-in-groups-of-adolescents-after-liver-and-kidney-transplantations
#17
A Jakubowska-Winecka, M Biernacka
The objective of the study was to determine the connection between parental attitudes and medication adherence and the selected aspects of treatment in groups of adolescents after kidney and liver transplantations, in comparison with adolescents with diabetes and inflammatory bowel disease. Attitudes were assessed using M. Plopa's Parental Attitudes Scale, which distinguishes 5 types of attitudes. Medication adherence was evaluated on the basis of the Morisky Medication Adherence Scale (MMAS-8), used with the author's consent...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177126/combined-liver-kidney-transplantation-in-children-single-center-experiences-and-long-term-results
#18
M Szymczak, P Kaliciński, G Kowalewski, M Markiewicz-Kijewska, D Broniszczak, H Ismail, M Stefanowicz, A Kowalski, J Rubik, I Jankowska, B Piątosa, J Teisseyre, R Grenda
Combined liver-kidney transplantation (CLKT) is a rare procedure in pediatric patients in which liver and kidney from 1 donor are transplanted to a recipient during a single operation. The aim of our study was to analyze indications and results of CLKT in children. MATERIALS AND METHODS: Between 1990 and 2017 we performed 722 liver transplantations in children; we performed 920 kidney transplantations in children since 1984. Among them, 25 received CLKT. Primary diagnosis was fibro-polycystic liver and kidney disease in 17 patients, primary hyperoxaluria type 1 in 6 patients, and atypical hemolytic uremic syndrome-related renal failure in 2 children...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177125/reconstruction-of-multiple-renal-arteries-during-simultaneous-pancreas-and-kidney-transplantation-a-case-report
#19
A Surowiecka-Pastewka, M Matejak-Górska, M Frączek, M Durlik
We present a case of a multiple renal artery reconstruction during simultaneous pancreas and kidney transplantation. The kidney graft had 6 renal arteries, the aorta patch was 10 cm long, and there were two renal veins. To perform anastomoses to the left external iliac vessels we had to reconstruct the renal arterial and venal patches. The results of the transplantation were very good. Both grafts had satisfactory function, even though a control computed tomography performed a year after transplantation revealed infarction of a lower renal pole...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30177124/analysis-of-hospitalizations-in-simultaneous-pancreas-kidney-transplant-recipients-a-single-center-experience-in-poland
#20
M Czerwińska, M S Gniewkiewicz, J Gozdowska, J Wyzgał, T Grochowiecki, S Nazarewski, M Kosieradzki, M Durlik
BACKGROUND: End-stage renal disease due to type 1 diabetes mellitus appears to be a regular indication for simultaneous pancreas and kidney transplantation (SPKT). Although transplantation improves a patient's health condition, it does not mean that all complications will be eliminated. METHODS: We performed a retrospective analysis of 73 patients who underwent SPKT and follow-up between 1988 and 2015 at our institute. The number, duration, and reasons for hospitalization at 1, 5, 10, and 15 years after SPKT were analyzed...
September 2018: Transplantation Proceedings
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