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Stem Cells and Development

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https://www.readbyqxmd.com/read/28810819/low-cll-1-expression-is-a-novel-adverse-predictor-in-123-patients-with-de-novo-cd34-acute-myeloid-leukemia
#1
Jin Wang, Yanyu Wang, Wen-Lian Chen, Jie Hao, Zhanyun Liu, Xiangqin Weng, Yan Sheng, Jing Wu, Yongmei Zhu, Bing Chen, Shu-Min Xiong, Yu Chen, Qiusheng Chen, Hui-Ping Sun, Junmin Li
Recent reports state that C-type lectin-like molecule-1 (CLL-1) in acute myeloid leukemia (AML) is expressed primarily on myeloid cells, but there is still no investigation about its prognostic significance on leukemic blast compartment. Hence, this study aimed to evaluate the prognostic value of CLL-1 in 123 patients with de novo CD34+ non-M3 AML. Multiparameter flow cytometry was used to assess the expression of CLL-1 on immature compartment in AML and control groups. We found that CLL-1 expression level on blast compartment was closely linked to clinical characteristics, treatment response and survival outcome of patients...
August 16, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28708036/amniotic-fluid-cells-show-higher-pluripotency-related-gene-expression-than-allantoic-fluid-cells
#2
Debora Kehl, Melanie Generali, Sabrina Görtz, Diego Geering, Jaroslav Slamecka, Simon P Hoerstrup, Ulrich Bleul, Benedikt Weber
Amniotic fluid represents an abundant source of multipotent stem cells, referred as broadly multipotent given their differentiation potential and expression of pluripotency-related genes. However, the origin of this broadly multipotent cellular fraction is not fully understood. Several sources have been proposed so far, including embryonic and extraembryonic tissues. In this regard, the ovine developmental model uniquely allows for direct comparison of fetal fluid-derived cells from two separate fetal fluid cavities, the allantois and the amnion, over the entire duration of gestation...
August 14, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28805158/pp2a-regulatory-subunit-b55%C3%AE-is-a-gatekeeper-of-osteoblast-maturation-and-lineage-maintenance
#3
Anastassia Serguienko, Robert Hanes, Iwona Grad, Meng-Yu Wang, Ola Myklebost, Else Munthe
Bone marrow mesenchymal stem cells (BM MSCs) mediate skeletal remodeling by differentiating into osteoblasts. However, this remodeling is impaired with aging as well as following long-term glucocorticoid treatment, resulting in osteoporosis. Here we report a novel factor of osteoblast differentiation - PP2A regulatory subunit B55γ. We show that B55γ is induced by glucocorticoid receptor (GR) in human primary BM MSCs during differentiation to osteoblast, but not to adipocytes, and is required for osteoblast morphogenesis and mineralization...
August 12, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28805145/pdgf-vegf-related-receptor-affects-transglutaminase-activity-to-control-cell-migration-during-crustacean-hematopoiesis
#4
Kingkamon Junkunlo, Chadanat Noonin, Kenneth Söderhäll, Irene Söderhäll
The platelet-derived growth factor (PDGF) receptor, a tyrosine kinase receptor whose ligand is PDGF, is crucial in the transduction of extracellular signals into cells and mediates numerous processes, such as cell proliferation, differentiation, survival and migration. We demonstrate the important roles of a receptor tyrosine kinase related to the PDGF/VEGF family protein (PVR) in controlling hematopoietic progenitor cell migration by affecting extracellular transglutaminase (TGase) activity. Pl_PVR1, GenBank accession number KY444650, is highly expressed in hemocytes and the hematopoietic tissue (HPT)...
August 12, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28795648/human-embryonic-stem-cell-derived-cardiomyocytes-self-arrange-with-areas-of-different-subtypes-during-differentiation
#5
Maj Linea Vestergaard, Søren J Grubb, Karen Koefod Rasmussen, Zoe Anderson-Jenkins, Kristina Grunnet-Lauridsen, Kristine Calloe, Christian Clausen, Søren T Christensen, Kjeld Møllgård, Claus Yding Andersen
The derivation of functional cardiomyocytes (CMs) from human embryonic stem cells (hESC) represents a unique way of studying human cardiogenesis, including the development of CM subtypes. In this study, we investigated the development and organization of CMs derived from hESCs (hESC-CMs) and examined how the expression of CM subtypes correspond to human in vivo cardiogenesis. Beating clusters were used to determine cardiac differentiation, which was evaluated by the expression of cardiac genes GATA4 and TNNT2 and subcellular localization of GATA4 and NKX2...
August 10, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28679316/mesenchymal-stem-multipotent-stromal-cells-from-human-decidua-basalis-reduce-endothelial-cell-activation
#6
Manal A Alshabibi, Al Joharah Al Huqail, Tanvir Khatlani, Fawaz M Abomaray, Ahmed S Alaskar, Abdullah O Alawad, Bill Kalionis, Mohamed Hassan Abumaree
Recently, we reported the isolation and characterization of mesenchymal stem cells from the decidua basalis of human placenta (DBMSCs). These cells express a unique combination of molecules involved in many important cellular functions, which make them good candidates for cell-based therapies. The endothelium is a highly specialized, metabolically active interface between blood and the underlying tissues. Inflammatory factors stimulate the endothelium to undergo a change to a proinflammatory and procoagulant state (ie, endothelial cell activation)...
August 3, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28665233/decellularization-of-whole-human-liver-grafts-using-controlled-perfusion-for-transplantable-organ-bioscaffolds
#7
Monique M A Verstegen, Jorke Willemse, Sjoerd van den Hoek, Gert-Jan Kremers, Theo M Luider, Nick A van Huizen, Francois E J A Willemssen, Herold J Metselaar, Jan N M IJzermans, Luc J W van der Laan, Jeroen de Jonge
Liver transplantation is the only effective treatment for end-stage liver disease, but absolute donor shortage remains a limiting factor. Recent advances in tissue engineering focus on generation of native extracellular matrix (ECM) by decellularized complete livers in animal models. Although proof of concept has been reported for human livers, this study aims to perform whole liver decellularization in a clinically relevant series using controlled machine perfusion. In this study, we describe a mild nondestructive decellularization protocol, effective in 11 discarded human whole liver grafts to generate constructs that reliably maintain hepatic architecture and ECM components using machine perfusion, while completely removing cellular DNA and RNA...
July 31, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28756741/modulation-of-mitochondrial-dna-copy-number-to-induce-hepatocytic-differentiation-of-human-amniotic-epithelial-cells
#8
Vijesh Vaghjiani, Jason E Cain, William Lee, Vijayaganapathy Vaithilingam, Bernard E Tuch, Justin St John
Mitochondrial DNA (mtDNA) copy number is tightly regulated during pluripotency and differentiation. There is increased demand of cellular ATP during differentiation for energy intensive cell types such as hepatocytes and neurons to meet the cell's functional requirements. During hepatocyte differentiation, mtDNA copy number must be synchronously increased to generate sufficient ATP through oxidative phosphorylation. Unlike bone marrow mesenchymal cells (BM-MSC), mtDNA copy number failed to increase by 28 days of differentiation of Human Amniotic Epithelial Cells (hAEC) into Hepatocyte-Like Cells (HLC) despite their expression of some end stage hepatic markers...
July 29, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28610554/slc7a11-overexpression-in-glioblastoma-is-associated-with-increased-cancer-stem-cell-like-properties
#9
Monika D Polewski, Rosyli F Reveron-Thornton, Gregory A Cherryholmes, Georgi K Marinov, Karen S Aboody
System xc(-) is a sodium-independent electroneutral transporter, comprising a catalytic subunit xCT (SLC7A11), which is involved in importing cystine. Certain cancers such as gliomas upregulate the expression of system xc(-), which confers a survival advantage against the detrimental effects of reactive oxygen species (ROS) by increasing generation of the antioxidant glutathione. However, ROS have also been shown to function as targeted, intracellular second messengers in an array of physiological processes such as proliferation...
July 27, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28747098/effect-of-mesenchymal-stromal-cells-on-t-cells-in-septic-context-immuno-suppression-or-immuno-stimulation
#10
Sebastien Le Burel, Cédric Thépenier, Laetitia Boutin, Jean-Jacques Lataillade, Juliette Peltzer
Sepsis is a complex process including a first wave of damage partially due to the body's response to pathogens, followed by a phase of immune cell dysfunction. The efficacy of a pharmacological approach facing a rapidly evolving system implies a perfect timing of administration, this difficulty could explain recent failure of clinical trials. MSC are usually defined as immunosuppressive, and their beneficial effects in preclinical models of acute sepsis have been shown to rely partly on such ability. If non regulated, this phenotype could be harmful in the immunosuppressed context arising hours after sepsis onset...
July 26, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28657463/transplantation-of-human-dental-pulp-derived-stem-cells-or-differentiated-neuronal-cells-from-human-dental-pulp-derived-stem-cells-identically-enhances-regeneration-of-the-injured-peripheral-nerve
#11
Imran Ullah, Ju-Mi Park, Young-Hoon Kang, June-Ho Byun, Dae-Geon Kim, Joo-Heon Kim, Dong-Ho Kang, Gyu-Jin Rho, Bong-Wook Park
Human dental mesenchymal stem cells isolated from the dental follicle, pulp, and root apical papilla of extracted wisdom teeth have been known to exhibit successful and potent neurogenic differentiation capacity. In particular, human dental pulp-derived stem cells (hDPSCs) stand out as the most prominent source for in vitro neuronal differentiation. In this study, to evaluate the in vivo peripheral nerve regeneration potential of hDPSCs and differentiated neuronal cells from DPSCs (DF-DPSCs), a total of 1 × 10(6) hDPSCs or DF-hDPSCs labeled with PKH26 tracking dye and supplemented with fibrin glue scaffold and collagen tubulization were transplanted into the sciatic nerve resection (5-mm gap) of rat models...
July 25, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28728472/the-identification-of-interferon-%C3%AE-as-a-key-supportive-factor-for-retinal-differentiation-of-murine-mesenchymal-stem-cells
#12
Barbora Hermankova, Jan Kossl, Eliska Javorkova, Pavla Bohacova, Michaela Hajkova, Alena Zajicova, Magdalena Krulova, Vladimir Holan
Retinal disorders represent the main cause of decreased quality of vision and even blindness worldwide. The loss of retinal cells causes irreversible damage of the retina and there are currently no effective treatment protocols for most retinal degenerative diseases. A promising approach for the treatment of retinal disorders is represented by stem cell-based therapy. The perspective candidates are mesenchymal stem cells (MSCs) which can differentiate into multiple cell types and produce a number of trophic and growth factors...
July 20, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28599575/suppression-of-microrna-34a-expression-in-the-myocardium-protects-against-ischemia-reperfusion-injury-through-silent-information-regulator-1-protective-pathway
#13
Bi-Cheng Fu, Ji-Lu Lang, Dong-Yang Zhang, Lu Sun, Wei Chen, Wei Liu, Kai-Yu Liu, Chong-Yi Ma, Shu-Lin Jiang, Ren-Ke Li, Hai Tian
MicroRNA-34a (miR-34a) is expressed in the myocardium and expression is altered after myocardial injury. We investigated the effects of miR-34a on heart function after ischemia-reperfusion (IR) injury. Cardiomyocytes were isolated from neonatal rat hearts and simulated IR injury was induced in vitro. Following IR injury in rats, infarct size was measured and left ventricular (LV) function was evaluated using echocardiography. Protein expression of silent information regulator 1 (SIRT1), acetylated p53 (ac-p53), Bcl-2 and Bax, and miR-34a and SIRT1 gene levels were analyzed...
July 20, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28715970/scaffold-in-scaffold-potential-to-induce-growth-and-differentiation-of-cardiac-progenitor-cells
#14
Matteo Ciocci, Federico Mochi, Felicia Carotenuto, Emilia Di Giovanni, Paolo Prosposito, Roberto Francini, Fabio De Matteis, Igor Reshetov, Mauro Casalboni, Sonia Melino, Paolo Di Nardo
The design of reliable biocompatible and biodegradable scaffolds remains one of the most important challenges for tissue engineering. In fact, properly designed scaffolds must display an adequate and interconnected porosity to facilitate cell spreading and colonization of the inner layers, and must release physical signals concurring to modulate cell function to ultimately drive cell fate. In the present study, a combination of optimal mechanical and biochemical properties has been considered to design a one-component 3D multitextured hydrogel scaffold in order to favor cell-scaffold interactions...
July 17, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28707964/roles-of-diffusion-dynamics-in-stem-cell-signaling-and-three-dimensional-tissue-development
#15
Richard J McMurtrey
Recent advancements in the ability to construct three-dimensional (3D) tissues and organoids from stem cells and biomaterials have not only opened abundant new research avenues in disease modeling and regenerative medicine but also have ignited investigation into important aspects of molecular diffusion in 3D cellular architectures. This paper describes fundamental mechanics of diffusion with equations for modeling these dynamic processes under a variety of scenarios in 3D cellular tissue constructs. The effects of these diffusion processes and resultant concentration gradients are described in the context of the major molecular signaling pathways in stem cells that both mediate and are influenced by gas and nutrient concentrations, including how diffusion phenomena can affect stem cell state, cell differentiation, and metabolic states of the cell...
July 14, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28699415/a-scalable-approach-for-the-generation-of-human-pluripotent-stem-cell-derived-hepatic-organoids-with-sensitive-hepatotoxicity-features
#16
Malte Sgodda, Zhen Dai, Robert Zweigerdt, Amar D Sharma, Michael Ott, Tobias Cantz
The quest for physiologically-active human hepatocyte-like cells for in vitro research and drug screening is high. The recent progress in the field of pluripotent stem cell (PSC) - derived hepatic cells within the last decade brings those cells closer to applications in translational medicine. However, the classical two-dimensional (2D) cell culture systems are of limited use, because relevant cell-cell interactions based on cell polarity, which is a major prerequisite for proper hepatic cell metabolisms, are not provided...
July 12, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28693365/behavior-of-xeno-transplanted-undifferentiated-human-induced-pluripotent-stem-cells-is-impacted-by-microenvironment-without-evidence-of-tumors
#17
Veronica Martinez-Cerdeno, Bonnie Barrilleaux, Ashley McDonough, Jeanelle Ariza, Benjamin Yuen, Priyanka Somanath, Catherine Le, Craig Steward, Kayla Horton, Paul Knoepfler
Human pluripotent stem cells (hPSC) have great clinical potential via the use of their differentiated progeny, a population in which there is some concern over risks of tumorigenicity or other unwanted cellular behavior due to residual hPSC. Preclinical studies using human stem cells are most often performed within a xenotransplant context. Here, we sought to measure how undifferentiated hPSC behave following xenotransplant. We directly transplanted undifferentiated human induced pluripotent and embryonic stem cells (hIPSC; hESC) into the adult mouse brain ventricle and analyzed their fates...
July 10, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28679315/bone-marrow-mesenchymal-stem-cell-derived-cd63-exosomes-transport-wnt3a-exteriorly-and-enhance-dermal-fibroblast-proliferation-migration-and-angiogenesis-in-vitro
#18
Jeffrey D McBride, Luis Menocal-Rodriguez, Ambar Candanedo, Wellington Guzman, Marta Garcia-Contreras, Evangelos Van Badiavas
Wnts are secreted glycoproteins that regulate stem cell self-renewal, differentiation, and cell-to-cell communication during embryonic development and in adult tissues. Bone marrow mesenchymal stem cells (BM-MSCs) have been shown to stimulate dermis repair and regeneration; however, it is unclear how BM-MSCs may modulate downstream Wnt signaling. While recent reports implicate that Wnt ligands and Wnt messenger RNAs (such as Wnt4) exist within the interior compartment of exosomes, it has been debated whether or not Wnts exist on the exterior surface of exosomes to travel in the extracellular space...
July 5, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28679310/protein-and-molecular-characterisation-of-a-clinically-compliant-amniotic-fluid-stem-cell-derived-extracellular-vesicle-fraction-capable-of-accelerating-muscle-regeneration-through-the-enhancement-of-angiogenesis
#19
Ben Mellows, Robert Mitchell, Manuela Antonioli, Oliver Kretz, David Chamber, Marie-Theres Zeuner, Bernd Denecke, Luca Musante, Durrgah Ramachandra, Florence Debacq-Chainiaux, Harry Holthofer, Barbara Joch, Steve Ray, Darius Widera, Anna L David, Tobios B Huber, Joern Dengjel, Paolo De Coppi, Ketan Patel
The secretome of human amniotic fluid stem cells (AFSC) has great potential as a therapeutic agent in regenerative medicine. However it must be produced in a clinically compliant manner before it can be used in humans. Here we developed a means of producing a biologically active secretome from AFSCs that is free of all exogenous molecules. We demonstrate that the full secretome is capable of promoting stem cell proliferation, migration and protection of cells against senescence. Furthermore, it has significant anti-inflammatory properties...
July 5, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28665183/one-stage-positron-emission-tomography-and-magnetic-resonance-imaging-to-assess-mesenchymal-stem-cell-survival-in-a-canine-model-of-intervertebral-disc-degeneration
#20
Donghua Hang, Fan Li, Wenjun Che, Xiaofeng Wu, Yao Wan, Jiandong Wang, Yanping Zheng
Intervertebral disc (IVD) degeneration is a major health problem. Although mesenchymal stem cells (MSCs) have been used to promote IVD regeneration, the actual survival time of implanted MSCs in IVDs has never been studied non-invasively and continuously in vivo. To investigate survival of implanted MSCs in vivo, this study used a canine model of degenerated IVD and MSCs transfected with a mutant herpes simplex type-1 virus thymidine kinase (HSV1-sr39tk) and labelled with magnetic iron oxide nanoparticles (MION)...
June 30, 2017: Stem Cells and Development
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