journal
MENU ▼
Read by QxMD icon Read
search

Stem Cells and Development

journal
https://www.readbyqxmd.com/read/27899062/scleraxis-is-essential-for-tendon-differentiation-by-equine-embryonic-stem-cells-and-in-equine-fetal-tenocytes
#1
Emma Bavin, Francesca Atkinson, Tom Barsby, Debbie Guest
The transcription factor scleraxis is required for tendon development and is upregulated during embryonic stem cell (ESC) differentiation into tenocytes. However, its role beyond early embryonic development is not defined. We utilised a short hairpin RNA to knockdown scleraxis expression in ESCs, adult and fetal tenocytes. No effect on growth or morphology was observed in 2D cultures. However, scleraxis knockdown in fetal tenocytes significantly reduced COL1A1, COMP and SOX9 gene expression. Scleraxis knockdown in adult tenocytes had no effect on the expression of these genes...
November 29, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27784195/mitochondrial-spare-respiratory-capacity-is-negatively-correlated-with-nuclear-reprogramming-efficiency
#2
Yan Zhou, Rasha Abdelkadhem Al-Saaidi, Paula Fernandez-Guerra, Kristine K Freude, Rikke Katrine Jentoft Olsen, Uffe Birk Jensen, Niels Gregersen, Poul Hyttel, Lars Bolund, Lars Aagaard, Peter Bross, Yonglun Luo
Nuclear reprogramming efficiency has been shown to be highly variable among different types of somatic cells and different individuals, yet the underlying mechanism remains largely unknown. Several studies have shown that reprogramming of fibroblasts into induced pluripotent stem cells (iPSCs) requires remodeling of mitochondria and a metabolic shift from an oxidative state to a more glycolytic state. In this study, we evaluated the nuclear reprogramming efficiency in relation to mitochondrial bioenergetic parameters of fibroblasts from seven different human individuals...
November 28, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27881051/mechanisms-of-immune-suppression-utilized-by-canine-adipose-and-bone-marrow-derived-mesenchymal-stem-cells
#3
Lyndah Chow, Valerie Johnson, Jonathan W Coy, Daniel P Regan, Steven W Dow
Mesenchymal stem cells (MSC) from rodents and humans have been shown to suppress T cells by distinct primary pathways, with NO-dependent pathways dominating in rodents and IDO-dependent pathways dominating in humans. However, the immune suppressive pathways utilized by canine MSC have not been as thoroughly studied, nor have BM-MSC and Ad-MSC been directly compared for their immune modulatory potency or pathway utilization. Therefore, canine BM-MSC and Ad-MSC were generated in vitro and their potency in suppressing T cell proliferation and cytokine production was compared, as well as differential gene expression...
November 23, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27863459/properties-of-human-embryonic-stem-cells-and-their-differentiated-derivatives-depend-on-non-histone-dna-binding-hmgb1-and-hmgb2-proteins
#4
Alireza J Bagherpoor, Dasa Dolezalova, Tomas Barta, Martin Kucirek, Soodabeh A Sani, Milan Esner, Michaela Kunova Bosakova, Vladimir Vinarsky, Lucie Peskova, Ales Hampl, Michal Stros
HMGB1 and HMGB2 proteins have been implicated in numerous cellular processes, including proliferation, differentiation, apoptosis, and tumor growth. It is unknown whether they are involved in regulating the typical functions of pluripotent human embryonic stem cells (hESCs) and/or those of the differentiated derivatives of hESCs. Using inducible, stably transfected hESCs capable of the shRNA-mediated knockdown of <i>HMGB1</i> and <i>HMGB2</i>, we provide evidence that the downregulation of HMGB1 and/or HMGB2 in undifferentiated hESCs does not affect the stemness of the cells and induces only minor changes to the proliferation rate, cell-cycle profile, and apoptosis...
November 18, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27855539/mouse-p2y4-nucleotide-receptor-is-a-negative-regulator-of-cardiac-adipose-derived-stem-cell-differentiation-and-cardiac-fat-formation
#5
Anne Lemaire, Marion Vanorlé, Michael Horckmans, Larissa di Pietrantonio, Sophie Clouet, Bernard Robaye, Jean-Marie Boeynaems, Didier Communi
Cardiac adipose-derived stem cells (cASCs) have the ability to differentiate into multiple cell lineages giving them a high potential for use in regenerative medicine. Cardiac fat tissue still raises many unsolved questions related to its formation and features. P2Y nucleotide receptors have already been described as regulators of differentiation of bone-marrow derived stem cells but remain poorly investigated in cASCs. We defined here the P2Y4 nucleotide receptor as a negative regulator of cardiac fat formation and cASC differentiation...
November 17, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27788624/paradigm-shift-from-stem-cells-to-cell-free-regenerative-endodontic-procedures-a-critical-review
#6
Radovan Žižka, Jiří Šedý
Regenerative endodontic procedures of immature teeth with necrotic pulp have become a part of therapeutic endodontic spectrum and are considered as an alternative to calcium hydroxide or mineral trioxide aggregate apexification. In last decade, numerous case reports and series with usage of cell-free approaches known as revascularization, revitalization, or maturogenesis have been published. This cell-free approach prevails in clinical regenerative endodontics because of its relative ease of performance, lower financial demands, and absence of complications such as tumorigenesis of used stem cells...
November 17, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27848279/a-transcriptional-switch-point-during-hematopoietic-stem-and-progenitor-cell-ontogeny
#7
Daisuke Sugiyama, Anagha Joshi, Kasem Kulkeaw, Keai Sinn Tan, Tomoko Yokoo-Inoue, Chiyo Mizuochi-Yanagi, Kaori Yasuda, Atsushi Doi, Tadafumi Iino, Masayoshi Itoh, Sayaka Nagao-Sato, Kenzaburo Tani, Koichi Akashi, Yoshihide Hayashizaki, Harukazu Suzuki, Hideya Kawaji, Piero Carninci, Alistair R R Forrest
During mammalian embryogenesis, hematopoietic stem and progenitor cells (HSPCs) originate from mesoderm-derived endothelial cells in the aorta-gonad-mesonephros (AGM) region and placenta. Later, HSPCs expand in fetal liver and migrate to bone marrow shortly before birth. Understanding global transcriptional regulation governing HSPC emergence from embryonic stem/induced pluripotent stem cells is necessary to devise clinical applications, such as novel transplantation approaches. Here, to assess transcriptional dynamics during development, we performed cap analysis of gene expression (CAGE) on 10 developmental murine HSPC populations isolated from the AGM region, placenta, fetal liver and bone marrow and identified 15,681 transcripts across HSPC ontogeny...
November 16, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27733093/biomarker-discovery-by-modeling-beh%C3%A3-et-s-disease-with-patient-specific-human-induced-pluripotent-stem-cells
#8
Mi-Young Son, Young-Dae Kim, Binna Seol, Mi-Ok Lee, Hee-Jun Na, Bin Yoo, Jae-Suk Chang, Yee Sook Cho
Behçet's disease (BD) is a chronic inflammatory and multisystemic autoimmune disease of unknown etiology. Due to the lack of a specific test for BD, its diagnosis is very difficult and therapeutic options are limited. Induced pluripotent stem cell (iPSC) technology, which provides inaccessible disease-relevant cell types, opens a new era for disease treatment. In this study, we generated BD iPSCs from patient somatic cells and differentiated them into hematopoietic precursor cells (BD iPSC-HPCs) as BD model cells...
November 16, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27842458/suppression-of-canine-dendritic-cell-activation-maturation-and-inflammatory-cytokine-release-by-mesenchymal-stem-cells-occurs-via-multiple-distinct-biochemical-pathways
#9
William Henry Wheat, Lyndah Chow, Jade N Kurihara, Daniel P Regan, Jonathan W Coy, Tracy L Webb, Steven W Dow
Mesenchymal stem cells (MSC) represent a readily accessible source of cells with potent immune modulatory activity. MSC can suppress ongoing inflammatory responses by suppressing T cell function, while fewer studies have examined the impact of MSC on dendritic cell function. The dog spontaneous disease model represents an important animal model with which to evaluate the safety and effectiveness of cellular therapy with MSC. This study evaluated the effects of canine MSC on the activation and maturation of canine monocyte-derived dendritic cells (DC), as well as mechanisms underlying these effects...
November 14, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27842457/wharton-s-jelly-mesenchymal-stem-cells-protect-the-immature-brain-in-rats-and-modulate-cell-fate
#10
Martin Mueller, Byron Oppliger, Marianne Jörger-Messerli, Ursula Reinhart, Eytan Barnea, Michael Paidas, Boris Kramer, Daniel Surbek, Andreina Schoeberlein
The development of a mammalian brain is a complex and long-lasting process. Not surprisingly, preterm birth is the leading cause of death in newborns and children. Advances in perinatal care reduced mortality but morbidity still represents a major burden. New therapeutic approaches are thus desperately needed. Given that mesenchymal stem/stromal cells (MSCs) emerged as a promising candidate for cell therapy, we transplanted MSCs derived from the Wharton`s jelly (WJ-MSCs) to reduce the burden of immature brain injury in a murine animal model...
November 14, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27736363/cardiac-mechanoperception-a-life-long-story-from-early-beats-to-aging-and-failure
#11
Maurizio Pesce, Elisa Messina, Isotta Chimenti, Antonio Paolo Beltrami
The life-long story of the heart starts concomitantly with primary differentiation events occurring in multipotent progenitors located in the so-called heart tube. This initially tubular structure starts a looping process, which leads to formation of the final four-chambered heart with a primary contribution of geometric and position-associated cell sensing. While this establishes the correct patterning of the final cardiac structure, it also provides feedbacks to fundamental cellular machineries controlling proliferation and differentiation, thus ensuring a coordinated restriction of cell growth and a myocyte terminal differentiation...
November 14, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27832737/oral-mucosa-harbors-a-high-frequency-of-endothelial-cells-a-novel-postnatal-cell-source-for-angiogenic-regeneration
#12
Jian Zhou, Jason Rogers, Scott Lee, DongMing Sun, Hai Yao, Jeremy J Mao, Kimi Y Kong
Endothelial progenitor cells/endothelial cells (EPCs/ECs) have great potential to treat pathological conditions such as cardiac infarction, muscle ischemia and bone fractures but isolation of EPC/ECs from existing cell sources is challenging due to their low endothelial cell frequency. We have isolated EP-like cells from rat oral mucosa and characterized their yield, immunophenotype, growth and in vivo angiogenic potential. The frequency of EP-like cells derived from oral mucosa is thousands of folds higher than EPCs derived from donor-match bone marrow samples...
November 10, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27829312/cudc-907-promotes-bone-marrow-adipocytic-differentiation-through-inhibition-of-histone-deacetylase-and-regulation-of-cell-cycle
#13
Dalia Ali, Hassan Alshammari, Radhakrishnan Vishnubalaji, Elna Paul Chalisserry, Rimi Hamam, Musaed Alfayez, Moustapha Kassem, Abdullah Aldahmash, Nehad M Alajez
The role of bone marrow adipocytes (BMA) in overall energy metabolism and their effects on bone mass is currently an area of intensive investigation. BMA differentiate from bone marrow stromal cells (BMSCs), however, the molecular mechanisms regulating BMA differentiation are not fully understood. Herein, we investigated the effect of CUDC-907, identified by screening an epigenetics small-molecule library, on adipocytic differentiation of human BMSCs, and determined its molecular mechanism of action. hMSCs exposed to CUDC-907 (500 nM) exhibited enhanced adipocytic differentiation (~2...
November 9, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27762728/mir-138-mir-222-overexpression-characterizes-the-mirnome-of-amniotic-mesenchymal-stem-cells-in-obesity
#14
Carmela Nardelli, Ilaria Granata, Laura Iaffaldano, Valeria D'Argenio, Valentina Del Monaco, Giuseppe Maria Maruotti, Daniela Omodei, Luigi Del Vecchio, Pasquale Martinelli, Francesco Salvatore, Mario Rosario Guarracino, Lucia Sacchetti, Lucio Pastore
Clinical findings and data obtained in animal models indicate that nutrient uptake and exposure to environmental agents during pregnancy may affect fetal/newborn gestational programming, thereby resulting in obesity and/or obesity-related disorders in offspring. Human amniotic mesenchymal stem cells (hA-MSCs) differentiate into adipocytes and are thus a suitable model to investigate adipocyte functions in obesity. The aim of this study was to elucidate the miRNome of hA-MSCs and its contribution to obesity in pregnancy...
November 8, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27821019/molecular-signatures-of-primary-human-spermatogonial-progenitors-and-its-neighboring-peritubular-stromal-compartment
#15
Abhishek Harichandan, Kavitha Sivasubramaniyan, Joerg Hennenlotter, Sven Poths, Jens Bedke, Stephan Kruck, Arnulf Stenzl, Hans-Jörg Bühring
In depth understanding of human spermatogenesis requires studying specific molecular signatures and interactions of spermatogonia with other testicular cell populations, for which isolation of pure populations of different cell types is crucial. Here we describe a technique to simultaneously enrich pure, multiple testicular cell populations including spermatogonia, endothelial (TECs) and perivascular mesenchymal stem/stromal cells (TMSCs) from testicular tissue by flow cytometry using a combination of defined markers...
November 7, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27821018/geminin-is-essential-for-pluripotent-cell-viability-during-teratoma-formation-but-not-for-differentiated-cell-viability-during-teratoma-expansion
#16
Diane C Adler-Wailes, Joshua A Kramer, Melvin DePamphilis
Pluripotent embryonic stem cells (ESCs) are unusual in that geminin has been reported to be essential either to prevent differentiation by maintaining expression of pluripotency genes, or to prevent DNA re-replication dependent apoptosis. To distinguish between these two incompatible hypotheses, immune-compromised mice were inoculated subcutaneously with ESCs harboring conditional Gmnn alleles alone or together with a tamoxifen dependent Cre recombinase gene. Mice were then injected with tamoxifen at various times during which the ESCs proliferated and differentiated into a teratoma...
November 7, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27819181/gene-therapy-for-bone-defects-in-oral-and-maxillofacial-surgery-a-systematic-review-and-meta-analysis-of-animal-studies
#17
Riham Fliefel, Jan Kühnisch, Michael Ehrenfeld, Sven Otto
Craniofacial bone defects are challenging problems for maxillofacial surgeons over the years. With the development of cell and molecular biology, gene therapy is a breaking new technology with the aim of regenerating tissues by acting as a delivery system for therapeutic genes in the craniofacial region rather than treating genetic disorders. A systematic review was conducted summarizing the articles reporting gene therapy in maxillofacial surgery to answer the question: Was gene therapy successfully applied to regenerate bone in the maxillofacial region? Electronic searching of online databases was performed in addition to hand-search of the references of the included articles...
November 7, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27712399/priming-equine-bone-marrow-derived-mesenchymal-stem-cells-with-proinflammatory-cytokines-implications-in-immunomodulation-immunogenicity-balance-cell-viability-and-differentiation-potential
#18
Laura Barrachina, Ana Rosa Remacha, Antonio Romero, Francisco José Vázquez, Jorge Albareda, Marta Prades, Jaime Gosálvez, Rosa Roy, Pilar Zaragoza, Inmaculada Martín-Burriel, Clementina Rodellar
Mesenchymal stem cells (MSCs) have a great potential for treating equine musculoskeletal injuries. Although their mechanisms of action are not completely known, their immunomodulatory properties appear to be key in their functions. The expression of immunoregulatory molecules by MSCs is regulated by proinflammatory cytokines; so inflammatory priming of MSCs might improve their therapeutic potential. However, inflammatory environment could also increase MSC immunogenicity and decrease MSC viability and differentiation capacity...
November 3, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27702398/mesenchymal-stem-cells-and-pericytes-to-what-extent-are-they-related
#19
Lucas Eduardo Botelho de Souza, Tathiane Maistro Malta, Simone Kashima Haddad, Dimas Tadeu Covas
Mesenchymal stem cells (MSCs) were initially identified as progenitors of skeletal tissues within mammalian bone marrow and cells with similar properties were also obtained from other tissues such as adipose and dental pulp. Although MSCs have been extensively investigated, their native behavior and in vivo identity remain poorly defined. Uncovering the in vivo identity of MSCs has been challenging due to the lack of exclusive cell markers, cellular alterations caused by culture methods, and extensive focus on in vitro properties for characterization...
November 3, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27676194/folic-acid-supports-pluripotency-and-reprogramming-by-regulating-lif-stat3-and-mapk-erk-signaling
#20
Tingyi Wei, Wenwen Jia, Zhen Qian, Liangyuan Zhao, Yangyang Yu, Lian Li, Chenxin Wang, Wei Zhang, Qi Liu, Dandan Yang, Guiying Wang, Zikang Wang, Kai Wang, Tao Duan, Jiuhong Kang
Pluripotent stem cells act as an excellent cell source for disease therapy because of its specific characteristics of self-renewal and differentiation. Pluripotent stem cells are heterogeneous, consisting of naive stem cells as well as primed epiblast stem cells. However, the strategies and mechanisms of maintaining naive pluripotent stem cells remain unclear. In this study, we found that folic acid (FA) sustained mouse embryonic stem cell (ESC) pluripotency and enabled long-term maintenance of the naive state of ESCs under CHIR99021 conditions...
November 3, 2016: Stem Cells and Development
journal
journal
20245
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"