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Marina Makrecka-Kuka, Stanislava Korzh, Karlis Vilks, Reinis Vilskersts, Helena Cirule, Maija Dambrova, Edgars Liepinsh
Significant impairments in mitochondrial function are associated with the development of multi-organ failure in sepsis/endotoxaemia, but the data on the dynamics of simultaneous mitochondrial impairment in multiple organs are limited. The aim of this study was to evaluate the changes in heart, brain and kidney mitochondrial function in an experimental model of lipopolysaccharide (LPS)-induced endotoxaemia.Samples were collected 4 and 24 h after single injection of LPS (10 mg/kg) in mice. Marked increases in inflammation-related gene expression were observed in all studied tissues 4 h after LPS administration...
January 9, 2019: Shock
Nejla Tilouche, Oussama Jaoued, Habiba Ben Sik Ali, Rim Gharbi, Mohamed Fekih Hassen, Souheil Elatrous
OBJECTIVES: the best modality of administration of hydrocortisone during septic shock has been poorly evaluated and the guidelines remain unclear in this respect. This study aimed to compare bolus of hydrocortisone to a continuous infusion during septic shock. DESIGN: randomized controlled, open-label trial. SETTING: medical ICU of a university hospital. PATIENTS: adult patients with septic shock requiring more than 2 mg/h (approximately 33...
January 7, 2019: Shock
Haibing Sun, Qin Xie, Zhiyong Peng
PURPOSE: To assess the benefits and harms of fenoldopam for nephroprotective effects in adult patients undergoing cardiac surgery. METHODS: We conducted a systematic review with meta-analysis of randomized controlled trials (RCTs) comparing fenoldopam with placebo in cardiac surgery. Trials were systematically searched from Pubmed, EMBASE, CENTRAL and CNKI databases, up to July 30, 2018. A trial sequential analysis(TSA) was used to determine whether the present evidence was valid and conclusive for the primary outcomes...
December 26, 2018: Shock
Luis A Flores-Mejía, Graciela L Cabrera-Rivera, Eduardo Ferat-Osorio, Ismael Mancilla-Herrera, Rafael Torres-Rosas, Ilka B Boscó-Garate, Constantino López-Macías, Armando Isibasi, Arturo Cerbulo-Vazquez, Lourdes A Arriaga-Pizano
Sepsis is a life-threatening condition associated with failure of at least one organ in the presence of infection. Along with SIRS, the acute systemic inflammatory syndrome without documented infection, sepsis represents a main health problem in intensive care units around the world. Hypercytokinemia and overexpression of activation-markers on leukocytes are frequently reported in SIRS/sepsis. Leukocyte functions including antibody mediated-phagocytosis, pathogen recognition and migration appear to be disabled in SIRS/septic patients...
December 26, 2018: Shock
Madathilparambil V Suresh, Sanjay Balijepalli, Boya Zhang, Vikas Vikram Singh, Samantha Swamy, Sreehari Panicker, Vladislov A Dolgachev, Chitra Subramanian, Sadeesh K Ramakrishnan, Bivin Thomas, Tejeshwar C Rao, Matthew J Delano, David Machado-Aranda, Yatrik M Shah, Krishnan Raghavendran
Acid aspiration-induced lung injury is a common disease in the intensive care unit (ICU) and acute respiratory distress syndrome (ARDS). Hypoxia-inducible factor (HIF)-1α is a major transcription factor responsible for regulating the cellular response to changes in oxygen tension. A clear understanding of the function of HIF-1α in lung inflammatory response is currently lacking. Here, we sought to determine the role of HIF-1α in type 2 alveolar epithelial cells (AEC) in the generation of the acute inflammatory response following gastric aspiration (GA)...
December 24, 2018: Shock
Andrew W Wang, David M Cauvi, Dennis Hawisher, Tony Reyes, Raul Coimbra, Stephen Bickler, Antonio De Maio
The omentum is a large mesenchymal fibro-fatty tissue with remarkable healing capability. It is also rich in immune cells, including macrophages, and lymphocytes, within particular structures named milky spots. Clinical observations indicate a high incidence of peritonitis after removal of the omentum suggesting that it may play a role in sepsis. To test this possibility, male CD-1 mice underwent simultaneous omentectomy and CLP, omentectomy-sham operation and CLP alone, and mortality was documented within 72 h post the insults...
December 21, 2018: Shock
Christoph Emontzpohl, Christian Stoppe, Alexander Theißen, Christian Beckers, Ulf P Neumann, Georg Lurje, Cynthia Ju, Jürgen Bernhagen, René H Tolba, Zoltan Czigany
BACKGROUND: Macrophage migration inhibitory factor (MIF) is an important stress-regulating mediator of acute ischemia/reperfusion (I/R) injury and ischemic conditioning. The present study aimed to investigate whether MIF is involved in the effects of remote ischemic conditioning (RIC) in a rat model of orthotopic liver transplantation (OLT). METHODS: OLTs were performed in male Lewis rats (245-340 g). Recipients were allocated in a randomized fashion into three experimental groups: remote preconditioning-RIPC, remote postconditioning-RIPOST, control...
December 21, 2018: Shock
Yu-Peng Jian, Hao-Xiang Yuan, Kun-Hua Hu, Chao Chen, Yu-Quan Li, Yan Li, Tian-Xin Yang, Zhi-Jun Ou, Jing-Song Ou
We recently demonstrated that circulating microparticles(MPs) from patients with valvular heart diseases (VHD) subjected to cardiac surgery impaired endothelial function and vasodilation. However, it is unknown whether or not the protein composition of these circulating MPs actually changes in response to the disease and the surgery. Circulating MPs were isolated from age-matched control subjects (n = 50) and patients (n = 50) with VHD before and 72 h after cardiac surgery. Proteomics study was performed by liquid chromatography and mass spectrometry combined with isobaric tags for relative and absolute quantification technique...
December 21, 2018: Shock
Zeyu Li, Yifan Jia, Yang Feng, Ruixia Cui, Zi Wang, Kai Qu, Chang Liu, Jingyao Zhang
Sepsis, a life-threatening organ dysfunction due to a dysregulated response to infection, is a common complication of major surgery. Previous studies have showed that methane possesses protective properties. This study aims to investigate the protective effect of methane-rich saline (MRS) on sepsis-induced liver injury. In an in vivo experiment, C57BL/6 mice received cecal ligation and puncture to create a septic model followed by MRS treatment (10 ml/kg, ip treatment) 30 min and 12 h after the operation...
December 21, 2018: Shock
Aaron M Williams, Isabel S Dennahy, Umar F Bhatti, Ben E Biesterveld, Nathan Graham, Yongqing Li, Hasan B Alam
Trauma remains a leading cause of morbidity and mortality among all age groups in the United States. Hemorrhagic shock and traumatic brain injury (TBI) are major causes of preventable death in trauma. Initial treatment involves fluid resuscitation to improve the intravascular volume. Although crystalloids may provide volume expansion, they do not have any pro-survival properties. Furthermore, aggressive fluid resuscitation can provoke a severe inflammatory response and worsen clinical outcomes. Due to logistical constraints, however, definitive resuscitation with blood products is often not feasible in the pre-hospital setting - highlighting the importance of adjunctive therapies...
December 21, 2018: Shock
Natsue Kiyonaga, Takahiro Moriyama, Yuichi Kanmura
The mechanisms by which landiolol, an ultra-short-acting, selective β-1 blocker, could improve septic acute kidney injury (AKI) and how inflammation might affect mitochondrial function and cause the renal injury were examined. Male Wistar rats (250-300 g) were randomly allocated to three groups: a sham control group (n = 8); a lipopolysaccharide (LPS) group (n = 8); and an LPS + landiolol group (n = 8). LPS was administered intravenously at the start of the experiments; the LPS + landiolol group rats received LPS and continuous intravenous landiolol...
December 18, 2018: Shock
Qing-Rui Cui, Yi-Hong Ling, Shi-Hong Wen, Ke-Xuan Liu, Yu-Ke Xiang, Wen-Jing Yang, Jian-Tong Shen, Yun-Sheng Li, Bao-Long Yuan, Wen-Qi Huang
Fluid resuscitation is the first-line antishock treatment in severe acute pancreatitis (SAP). Currently, although mentions of complications related to aggressive fluid resuscitation are very frequent, a lack of proper handling of complications remains. One of the most important complications is intestinal barrier injury, including intestinal ischemia-reperfusion injury following aggressive fluid resuscitation. Once injured, the intestinal barrier may serve as the source of additional diseases, including systemic inflammatory response syndrome and multiple organ dysfunction syndrome, which aggravate SAP...
December 13, 2018: Shock
Laura Kitzmiller, John R Ledford, Paul W Hake, Michael O'Connor, Giovanna Piraino, Basilia Zingarelli
A serious consequence of sepsis is acute lung injury, whose severity is particularly impacted by the age of the patient. AMP-activated protein kinase (AMPK) is a crucial regulator of cellular metabolism, which controls mitochondrial biogenesis and autophagy. Here, we investigated the effect of pharmacological activation of AMPK with A769662 on lung injury by using a model that would preferably mimic the clinical condition of adult patients. Male C57BL/6 retired breeder mice (7-9 months old) were subjected to sepsis by cecal ligation and puncture (CLP)...
December 13, 2018: Shock
Lei Li, Yimei Zhang, Haihua Luo, Chenyang Huang, Shan Li, Aihua Liu, Yong Jiang
Long non-coding RNAs (lncRNAs), once thought to be transcriptional noise, have been recently shown to regulate a variety of biological processes. However, their roles in the inflammatory response are largely unexplored. In this study, we performed high-throughput sequencing to identify the profiles of mRNA and lncRNA transcriptomes in response to LPS stimulation, followed by a comprehensive bioinformatics analysis. We found a total of 325 lncRNAs and 1187 mRNAs to be significantly dysregulated in RAW264.7 cells stimulated with LPS (fold change>4...
November 29, 2018: Shock
Nikolaus Hofmann, Johannes Zipperle, Florian Brettner, Mohammad Jafarmadar, Mostafa Ashmwe, Claudia Keibl, Martin Ponschab, Ulrike Kipman, Arian Bahrami, Heinz Redl, Soheyl Bahrami, Valentin Fuhrmann, Herbert Schöchl
BACKGROUND: Plasma-based resuscitation showed protective effects on the endothelial glycocalyx compared to crystalloid resuscitation. There is paucity of data regarding the effect of coagulation factor concentrates (CFC) on the glycocalyx in hemorrhagic shock (HS). We hypothesized that colloid-based resuscitation supplemented with CFCs offers a therapeutic value to treat endothelial damage following HS. METHODS: Eighty-four rats were subjected to pressure controlled (mean arterial pressure (MAP) 30-35 mmHg) and lab guided (targeted cutoff: lactate >2...
November 29, 2018: Shock
Satoshi Fukuda, Koji Ihara, Clark R Andersen, Anita C Randolph, Christina L Nelson, Yaping Zeng, Jisoo Kim, Douglas S DeWitt, Jose D Rojas, Aristides Koutrouvelis, David N Herndon, Donald S Prough, Perenlei Enkhbaatar
Vascular hypo-responsiveness to vasopressors during septic shock is a challenging problem. This study is to test the hypothesis that reactive nitrogen species (RNS), such as peroxynitrite, are major contributing factors to vascular hypo-responsiveness in septic shock. We hypothesized that adjunct therapy with peroxynitrite decomposition catalyst (PDC) would reduce norepinephrine requirements in sepsis resuscitation. Fourteen female Merino sheep were subjected to a "two-hit" injury (smoke inhalation and endobronchial instillation of live methicillin-resistant Staphylococcus aureus [1...
November 28, 2018: Shock
Jun Hong, Hong-Wei Ge, Jing-Quan Liu, Ren-Hua Sun, Fei-Juan Kong
Although remote ischemic postconditioning (RIPC) was shown to confer cardioprotection against myocardial ischemia/reperfusion (I/R) injury in normal animals, whether RIPC-induced cardioprotection are altered in the presence of hypercholesterolemia, a comorbidity with acute myocardial infarction (AMI) patients, has yet to be determined. Normal or 2% cholesterol chow was fed to male C57BL/6J mice for 12 weeks to induce hypercholesterolemia, then normal or hypercholesterolemic murine hearts were exposed to AMI by coronary artery ligation...
November 28, 2018: Shock
David Peter Obert, Alexander Karl Wolpert, Sebastian Korff
BACKGROUND: Impaired function of the endoplasmic reticulum (ER) results in ER stress, an accumulation of proteins in the ER lumen. ER stress is a major contributor to inflammatory diseases and is part of the pathomechanism of ischemia-reperfusion injury (IRI). Since severe traumatic injury is often accompanied by remote organ damage and immune cell dysfunction, we investigated the influence of ER stress modulation on the systemic inflammatory response and liver damage after hemorrhagic shock and reperfusion (HS/R)...
November 28, 2018: Shock
Sai Saran, Mohan Gurjar, Afzal Azim, Prabhaker Mishra, Pralay S Ghosh, Arvind K Baronia, Banani Poddar, Ratender K Singh
BACKGROUND: Effect of prone positioning on acute hemodynamic changes (within 10 minutes) in acute respiratory distress syndrome (ARDS) has not been studied. METHODS: In this prospective observational study, hemodynamic assessment by trans-esophageal Doppler (TED) was done with the primary aim to measure early changes in cardiac index (CI), if any, after prone positioning in moderate to severe ARDS patients. A subgroup analysis was also done based on the response to passive leg raise (PLR)...
November 20, 2018: Shock
Hongkuan Fan, Andrew J Goodwin
No abstract text is available yet for this article.
November 20, 2018: Shock
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