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Reproductive Biomedicine Online

Francesca Romana Grati, Gloria Gallazzi, Lara Branca, Federico Maggi, Giuseppe Simoni, Yuval Yaron
The aim of this study was to devise an evidence-based scoring system for prioritizing mosaic aneuploid embryos for transfer. A retrospective analysis was performed of all sequential cytogenetic and molecular results on chorionic villi samples (n = 72,472) and products of conception (n = 3806) analysed at a single centre. The likelihood that a mosaic aneuploidy detected in chorionic villi samples will involve the fetus, the incidence of clinically significant fetal uniparental disomy in the presence of a mosaic in chorionic villi and the chance of the mosaicism culminating in miscarriage were used to generate a scoring system for prioritizing mosaic aneuploid embryos detected by preimplantation genetic screening...
February 9, 2018: Reproductive Biomedicine Online
Arnaud Reignier, Jenna Lammers, Paul Barriere, Thomas Freour
Embryo morphology assessment performs relatively poorly in predicting implantation. Embryo aneuploidy screening (PGS) has recently improved, but its clinical value is still debated, and the development of a cheap non-invasive method for the assessment of embryo ploidy status is a highly desirable goal. The growing implementation of time-lapse devices led some teams to test the effectiveness of morphokinetic parameters as predictors of embryo ploidy, with conflicting results. The aim of this study was to conduct a comprehensive review of the literature on the predictive value of morphokinetic parameters for embryo ploidy status...
February 1, 2018: Reproductive Biomedicine Online
Marina Sansinena, Maria Victoria Santos, Jorge Chirife, Noemi Zaritzky
Heat transfer during cooling and warming is difficult to measure in cryo-devices; mathematical modelling is an alternative method that can describe these processes. In this study, we tested the validity of one such model by assessing in-vitro development of vitrified and warmed bovine oocytes after parthenogenetic activation and culture. The viability of oocytes vitrified in four different cryo-devices was assessed. Consistent with modelling predictions, oocytes vitrified using cryo-devices with the highest modelled cooling rates had significantly (P < 0...
January 31, 2018: Reproductive Biomedicine Online
Zewu Li, Yueting Zhu, Hongchang Li, Wenjie Jiang, Hongbin Liu, Junhao Yan, Zi-Jiang Chen, Weiping Li
Polycystic ovary syndrome (PCOS) is characterized by hyperandrogenism, ovarian dysfunction and polycystic ovarian morphology. Leukaemia inhibitory factor (LIF) affects many reproductive activities, including follicular development, embryo implantation and growth. The aim of this study was to evaluate LIF concentrations in serum and follicular fluid of women with PCOS and controls who underwent IVF with embryo transfer (IVF-ET). Serum and follicular fluid LIF concentrations were lower in women with PCOS compared with controls...
January 20, 2018: Reproductive Biomedicine Online
Jesper Friis Petersen, Anders Nyboe Andersen, Bjarke Mirner Klein, Lisbeth Helmgaard, Joan-Carles Arce
Research has focused on optimizing luteal phase support and endometrial receptivity in ovarian stimulation cycles. In this study, serial endocrine measurements were taken in 600 patients after a gonadotrophin-releasing hormone antagonist stimulation protocol. On the day of blastocyst transfer, serum progesterone and oestradiol were similar irrespective of a subsequent positive or negative pregnancy test (median 99 ng/ml versus 103 ng/ml for progesterone, respectively) or a subsequent live birth or pregnancy loss...
January 17, 2018: Reproductive Biomedicine Online
Csaba Pribenszky, Anna-Maria Nilselid, Markus Montag
No abstract text is available yet for this article.
January 5, 2018: Reproductive Biomedicine Online
Wen Liu, Xuan Gao, Lei Yan, Hongli Liu, Ruimei Yu, Shidou Zhao, Jinlong Ma
To examine whether mutations of the CDK2 gene exist in Chinese men with non-obstructive azoospermia (NOA) with different histopathology, we recruited 175 Chinese men with idiopathic NOA who underwent testis biopsy, including hypospermatogenesis, germ cell maturation arrest and Sertoli cell only syndrome. Genomic DNA was extracted from peripheral blood samples. Subsequently, the seven exons of the CDK2 gene were amplified using polymerase chain reaction with specific primers, respectively. The polymerase chain reaction products were sequenced on an automated sequencer...
January 4, 2018: Reproductive Biomedicine Online
Vânia Costa Ribeiro, Samuel Santos-Ribeiro, Neelke De Munck, Panagiotis Drakopoulos, Nikolaos P Polyzos, Valerie Schutyser, Greta Verheyen, Herman Tournaye, Christophe Blockeel
The evaluation of endometrial thickness (EMT) is still part of standard cycle monitoring during IVF, despite the lack of robust evidence of any value of this measurement to predict little revalidation in contemporary medical practice; other tools, however, such as endocrine profile monitoring, have become increasingly popular. The aim of this study was to reassess whether EMT affects the outcome of a fresh embryo transfer in modern-day medicine, using a retrospective, single-centre cohort of 3350 IVF cycles (2827 women) carried out between 2010 and 2014...
January 3, 2018: Reproductive Biomedicine Online
Diego Marin, Rebekah Zimmerman, Xin Tao, Yiping Zhan, Richard T Scott, Nathan R Treff
Triploidy accounts for ~2% of natural pregnancies and 15% of cytogenetically abnormal miscarriages. This study aimed to validate triploidy detection in human blastocysts, its frequency and parental origin using genotyping data generated in parallel with chromosome copy number analysis by a targeted next generation sequencing (tNGS)-based comprehensive chromosome screening platform. Phase 1: diploid and triploid control samples were blinded, sequenced by tNGS and karyotype predictions compared for accuracy. Phase 2: tNGS was used to calculate the frequency of triploidy in 18,791 human blastocysts from trophectoderm (TE) biopsies...
January 2, 2018: Reproductive Biomedicine Online
Sarah Armstrong, Priya Bhide, Vanessa Jordan, Allan Pacey, Cynthia Farquhar
No abstract text is available yet for this article.
December 29, 2017: Reproductive Biomedicine Online
Kemal Ozgur, Hasan Bulut, Murat Berkkanoglu, Peter Humaidan, Kevin Coetzee
This retrospective cohort analysis compared the developmental competence of cryopreserved day-4 and 5 blastocysts, and investigated the effect of progesterone administration duration on the success of artificial frozen embryo transfers. Between October 2015 and March 2016, 868 intracytoplasmic sperm injection blastocyst cryo-all cycles were carried out, with 586 subsequently undergoing frozen embryo transfer. Of these, 243 were day-5 single blastocyst transfers (SBT) and 152 were day-4 SBT. Day-4 blastocysts were transferred on day-5 progesterone (day-4 group) and day-5 blastocysts were transferred on day-5 (short-protocol day-5 sub-group, n = 104) or day-6 (standard-protocol day-5 sub-group, n = 139) progesterone...
December 29, 2017: Reproductive Biomedicine Online
Roberto A Antunes, Ana C A Mancebo, Mila W Reginatto, Vitor A S Deriquehem, Patricia Areas, Enrrico Bloise, Maria I Chiamolera, Gabriel C M Ribeiro, Alysson R S Carvalho, Maria C B Souza, Tânia M Ortiga-Carvalho
Vitamin D receptor-knockout mice fail to produce mature oocytes, indicating vitamin D is crucial for folliculogenesis in mice. However, the actions of vitamin D during folliculogenesis remain unknown. This prospective study aimed to assess whether follicular fluid (FF) vitamin D (25OHD3) concentrations are related to specific responses to ovarian stimulation. Women undergoing ovarian stimulation for IVF participated in the study. FF 25OHD3 concentrations were assessed in the first follicle aspirate on oocyte retrieval day...
December 29, 2017: Reproductive Biomedicine Online
Luis Martínez-Granados, María Serrano, Antonio González-Utor, Nereyda Ortiz, Vicente Badajoz, María Luisa López-Regalado, Montserrat Boada, Jose A Castilla
An external quality-control programme for morphology-based embryo quality assessment, incorporating a standardized embryo grading scheme, was evaluated over a period of 5 years to determine levels of inter-observer reliability and agreement between practising clinical embryologists at IVF centres and the opinions of a panel of experts. Following Guidelines for Reporting Reliability and Agreement Studies, the Gwet index and proportion of positive (Ppos) and negative agreement were calculated. For embryo morphology assessment, a substantial degree of reliability was measured between the centres and the panel of experts (Gwet index: 0...
December 29, 2017: Reproductive Biomedicine Online
Marziyeh Tavalaee, Michail Nomikos, F Anthony Lai, Mohammad Hossein Nasr-Esfahani
Globozoospermia is characterized by the presence of 100% acrosomeless round-headed spermatozoa in an ejaculate. Failed fertilization after intracytoplasmic sperm injection (ICSI) is commonly reported for globozoospermic couples and can be overcome by artificial oocyte activation (AOA). Phospholipase C zeta (PLCζ) is one of the main sperm factors involved in oocyte activation and its low expression levels mainly account for fertilization failure. Deletion of the DPY19L2 gene is reported as a main genetic cause in over 70% of infertile men with globozoospermia...
December 29, 2017: Reproductive Biomedicine Online
Kay Neumann, Georg Griesinger
To evaluate the effect of follicular flushing on clinical outcomes (primary outcome: mean number of COCs) in poor response IVF patients a systematic literature review and meta-analysis was performed. The bibliographic databases OvidMedline (includes Pubmed), Cochrane Library and Web of Science were searched electronically for randomized controlled trials (RCTs) comparing follicular flushing with no flushing. Three RCTs with a total of 210 patients could be included. The mean number of COC did not increase with flushing (weighted mean difference: -0...
December 29, 2017: Reproductive Biomedicine Online
Andreas N Schüring, Beatrice Dahlhues, Anna Korte, Ludwig Kiesel, Ulf Titze, Birthe Heitkötter, Christian Ruckert, Martin Götte
Previous studies reported increased expression of the notch pathway-associated protein Musashi-1 in endometriosis. This case-control study investigates an association of the endometrial stem cell markers notch-1 and numb with endometriosis. Fifty-one endometriosis patients and 76 controls were recruited in the IVF unit and tertiary endometriosis referral centre of a university hospital. All subjects underwent transcervical endometrial biopsy and diagnostic laparoscopy. Expression of endometrial notch-1 and numb was assessed by immunostaining and correlated with clinical data...
December 28, 2017: Reproductive Biomedicine Online
Abraham N Morse, Wenyue Si, Shuang Qin, Huiying Liang
The aim of this study was to improve clinical decision-making for the identification of persistent ectopic pregnancy after linear salpingostomy. The study identified 854 laparoscopic salpingostomies performed between 2011 and 2016; 794 had a human chorionic gonadotrophin (HCG) <10 mIU/ml documented in the electronic medical record within 1 month after surgery ('successes'). Sixty (7%) received either methotrexate or repeat surgery for persistent ectopic pregnancy ('failures'). Five hundred and seventeen, including 46 'failures', had two or more immediate post-operative HCG measurements available...
December 28, 2017: Reproductive Biomedicine Online
Karen Peeraer, Jeroen Luyten, Carla Tomassetti, Sarah Verschueren, Carl Spiessens, Ann Tanghe, Christel Meuleman, Sophie Debrock, Eline Dancet, Thomas D'Hooghe
Ovarian stimulation with low-dose human menopausal gonadotrophin (HMG) is superior to clomiphene citrate in intrauterine insemination (IUI) cycles with respect to clinical pregnancy rate, but it is unclear whether HMG is also the more cost-effective option. The aim of this study was to compare the cost-effectiveness of ovarian stimulation with low-dose subcutaneously administred HMG (37.5-75 IU per day) to orally administred clomiphene citrate (50 mg/day from day 3-7) in an IUI programme for subfertile couples...
December 27, 2017: Reproductive Biomedicine Online
An Bracke, Kris Peeters, Usha Punjabi, David Hoogewijs, Sylvia Dewilde
Infertility affects approximately 15% of the couples wanting to conceive. In 30 - 40% of the cases the aetiology of male infertility remains unknown and is called idiopathic male infertility. When assisted reproductive technologies are used to obtain pregnancy, an adequate (epi)genetic diagnosis of male infertility is of major importance to evaluate if a genetic abnormality will be transmitted to the offspring. In addition, there is need for better diagnostic seminal biomarkers to assess the success rates of these assisted reproductive technologies...
December 27, 2017: Reproductive Biomedicine Online
Mina Alikani, Bart C J M Fauser, Richard Anderson, Juan Antonio García-Velasco, Martin Johnson
No abstract text is available yet for this article.
December 26, 2017: Reproductive Biomedicine Online
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