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Reproductive Biomedicine Online

Tetsuya Ishii
Genome editing based on site-directed nucleases facilitated efficient and versatile genetic modifications in human cells. However, recent reports, demonstrating CRISPR/Cas9-mediated genome editing in human embryos have raised profound concerns worldwide. This commentary explores the clinical justification and feasibility of reproductive medicine using germline genome editing. Despite the perceived utility of reproductive medicine for treating intractable infertility, it is difficult to justify germline genome editing from the perspective of the prospective child...
October 5, 2016: Reproductive Biomedicine Online
Enrico Papaleo, Luca Pagliardini, Valeria Stella Vanni, Diana Delprato, Patrizia Rubino, Massimo Candiani, Paola Viganò
A cost analysis covering direct healthcare costs relating to IVF freeze-all policy was conducted. Normal- and high- responder patients treated with a freeze-all policy (n = 63) compared with fresh transfer IVF (n = 189) matched by age, body mass index, duration and cause of infertility, predictive factors for IVF (number of oocytes used for fertilization) and study period, according to a 1:3 ratio were included. Total costs per patient (€6952 versus €6863) and mean costs per live birth were similar between the freeze-all strategy (€13,101, 95% CI 10,686 to 17,041) and fresh transfer IVF (€15,279, 95% CI 13,212 to 18,030)...
October 3, 2016: Reproductive Biomedicine Online
Yves J R Menezo, Erica Silvestris, Brian Dale, Kay Elder
The negative effect of oxidative stress on the human reproductive process is no longer a matter for debate. Oxidative stress affects female and male gametes and the developmental capacity of embryos. Its effect can continue through late stages of pregnancy. Metabolic disorders and psychiatric problems can also be caued by DNA methylation and epigenetic errors. Age has a negative effect on oxidative stress and DNA methylation, and recent observations suggest that older men are at risk of transmitting epigenetic disorders to their offspring...
September 27, 2016: Reproductive Biomedicine Online
Zahra Eelaminejad, Raha Favaedi, Niloofar Sodeifi, Mohammad Ali Sadighi Gilani, Maryam Shahhoseini
JMJD1A (jumonji domain-containing 1A), a known histone H3K9 demethylase, has been identified as a critical epigenetic regulator in male germ cells, activating the sperm chromatin-packaging genes encoding protamines (PRM) and transition proteins (TNP) required for spermatid elongation and condensation. This research investigated the expression pattern of JMJD1A protein in testicular biopsies of 79 infertile men who had undergone testicular sperm extraction. Samples were classified into obstructive azoospermia (OA, n = 26), round spermatid maturation arrest (SMA, n = 29) and Sertoli cell only syndrome (SCOS, n = 24)...
September 22, 2016: Reproductive Biomedicine Online
Maria Memtsa, Eric Jauniaux, Béatrice Gulbis, Netta C Nyrhinen, Davor Jurkovic
The aim of this study was to evaluate the use of biological serum markers, available routinely in most hospital clinical laboratories, in predicting successful outcomes of expectant management in women presenting with a missed miscarriage. This is a single centre observational prospective study over a 16-month period. Among the 490 women who consented to the study protocol, 83 presented with missed miscarriage during the first trimester of pregnancy and opted for expectant management. The mean gestation sac diameter and volume of the gestation sac were recorded during ultrasound examination...
September 21, 2016: Reproductive Biomedicine Online
Myrthe M van Dijk, Rosa Vissenberg, Peter H Bisschop, Feroza Dawood, Madelon van Wely, Mariëtte Goddijn, Roy G Farquharson
Thyroid disorders have been associated with recurrent miscarriage. Little evidence is available on the influence of subclinical hypothyroidism on live birth rates. In this cohort study, women who had experienced miscarriage and subclinical hypothyroidism (defined as thyroid-stimulating hormone >97.5th percentile mU/l with a normal thyroxine level) were investigated; the control group included women who had experienced recurrent miscarriage and normal thyroid function. Multivariable logistic regression was used to investigate the association of subclinical hypothyroidism...
September 20, 2016: Reproductive Biomedicine Online
Michael Feichtinger, Christian Göbl, Andrea Weghofer, Wilfried Feichtinger
The aim of this retrospective cohort study was to assess differences in infertility-related baseline characteristics and IVF outcome between European and Middle Eastern/North African (MENA) patients. Of 2703 patients undergoing their first IVF cycle, 2485 were Caucasian of European descent and 218 originated from the MENA region. MENA patients were significantly younger (30.6 versus 34.0 years, P < 0.001), less likely smokers, with higher body mass indexes. Infertility duration was longer in MENA patients (P < 0...
September 20, 2016: Reproductive Biomedicine Online
Si-Wei Wang, Song-Yang Zhong, Li-Jun Lou, Ze-Fu Hu, Hua-Yu Sun, Hai-Yan Zhu
The aim of this study was to investigate the effect of passive immunotherapy using intravenous immunoglobulin (IVIG) on unexplained recurrent spontaneous abortion (RSA). Live birth rates were analysed and binary data were calculated using risk ratio and 95% confidence interval. Meta-analysis of 11 studies showed that the difference in the live birth rate between the IVIG treatment and placebo groups was on the margin of significance (RR = 1.25, 95% CI 1.00 to 1.56, P = 0.05). Both cumulative and trial sequential meta-analyses indicated potential beneficial effect of IVIG but the evidence was inconclusive...
September 16, 2016: Reproductive Biomedicine Online
Alessandro D Genazzani
Studies over the last decade have demonstrated that some polycystic ovary syndrome (PCOS) patients have abnormal insulin sensitivity (insulin resistance), independently from being overweight or obese. This induces the risk of developing type 2 diabetes in such PCOS patients. The use of insulin sensitizers (i.e. metformin), reduces such metabolic, and most hormonal, impairments. As metformin often induces side effects, new integrative strategies have been proposed to treat insulin resistance, such as the use of inositols...
September 16, 2016: Reproductive Biomedicine Online
Emad A Taha, Sohair K Sayed, Hisham D Gaber, Hatem K Abdel Hafez, Nagwa Ghandour, Asmaa Zahran, Taymour Mostafa
The effect of being overweight on seminal variables was assesed in 165 fertile men. Participants were divided into three groups: fertile men with normal body mass index (BMI) (18.5-24.9 kg/m(2)), fertile overweight men (BMI 25-29.9 kg/m(2)) and fertile obese men (BMI >30 kg/m(2)). Medical history was taken, a clinical examination conducted. Semen analysis was undertaken and BMI measured. Seminal reactive oxygen species (ROS) was estimated by chemiluminescent assay, sperm vitality by the hypo-osmotic swelling test and sperm DNA fragmentation by propidium iodide staining with flowcytometry...
September 16, 2016: Reproductive Biomedicine Online
Cristina Camprubí, Albert Salas-Huetos, Riccardo Aiese-Cigliano, Anna Godo, Maria-Carme Pons, Giancarlo Castellano, Mark Grossmann, Walter Sanseverino, José I Martin-Subero, Nicolás Garrido, Joan Blanco
The influence of aberrant sperm DNA methylation on the reproductive capacity of couples has been postulated as a cause of infertility. This study compared the DNA methylation of spermatozoa of 19 fertile donors and 42 infertile patients using the Illumina 450K array. Clustering analysis of methylation data arranged fertile and infertile patients into two groups. Bivariate clustering analysis identified a differential distribution of samples according to the characteristics of seminogram and age, suggesting a possible link between these parameters and specific methylation profiles...
September 15, 2016: Reproductive Biomedicine Online
Fatma Uysal, Gokhan Akkoyunlu, Saffet Ozturk
DNA methylation is one of the epigenetic marks and plays critically important functions during spermatogenesis in mammals. DNA methylation is catalysed by DNA methyltransferase (DNMT) enzymes, which are responsible for the addition of a methyl group to the fifth carbon atom of the cytosine residues within cytosine-phosphate-guanine (CpG) and non-CpG dinucleotide sites. Structurally and functionally five different DNMT enzymes have been identified in mammals, including DNMT1, DNMT2, DNMT3A, DNMT3B and DNMT3L...
September 12, 2016: Reproductive Biomedicine Online
T T F Huang, K Chinn, T Kosasa, H J Ahn, B Kessel
Time-lapse imaging offers new tools to study dynamic processes of development such as blastocyst formation and expansion. This study quantitatively describes expansion in human blastocysts from donated oocytes. Measurements of hourly interval rate of changes in the blastocoel cross-sectional area revealed oscillatory pulses having 2-4 h periodicities. Two types of oscillations were distinguished. An E-Type ('expansion') had positive peak and positive or slightly negative trough interval rate of change values, and these characterized most of the expansion period...
September 12, 2016: Reproductive Biomedicine Online
Pamela Schendelaar, Sacha La Bastide-Van Gemert, Maas Jan Heineman, Karin J Middelburg, Jorien Seggers, Edwin R Van den Heuvel, Mijna Hadders-Algra
Research on cognitive and behavioural development of children born after assisted conception is inconsistent. This prospective study aimed to explore underlying causal relationships between ovarian stimulation, in-vitro procedures, subfertility components and child cognition and behaviour. Participants were singletons born to subfertile couples after ovarian stimulation IVF (n = 63), modified natural cycle IVF (n = 53), natural conception (n = 79) and singletons born to fertile couples (reference group) (n = 98)...
September 9, 2016: Reproductive Biomedicine Online
Ashley Moffett, Olympe Chazara, Francesco Colucci, Martin H Johnson
A distinctive type of (uterine) natural killer (NK) cell is present in the uterine decidua during the period of placental formation. Uterine NK cells express members of the killer immunoglobulin-like receptor (KIR) family that bind to parental HLA-C molecules on the invading placental trophoblast cells. The maternal KIR genes and their fetal ligands are highly variable, so different KIR/HLA-C genetic combinations occur in each pregnancy. Some women only possess inhibitory KIR genes, whereas other women also express activating KIR genes...
September 5, 2016: Reproductive Biomedicine Online
Joe Leigh Simpson, Anver Kuliev
No abstract text is available yet for this article.
August 31, 2016: Reproductive Biomedicine Online
K Birch Petersen, NlC Freiesleben
No abstract text is available yet for this article.
August 24, 2016: Reproductive Biomedicine Online
Wenting Wang, Lei Cheng, Jiangtao Zhang, Yingying Qin, Shidou Zhao, Zi-Jiang Chen
Insights into common genetic susceptibility between primary ovarian insufficiency (POI) and natural or early menopause have delivered an innovative way of assessing the genetic mechanisms involved in POI. PRIM1 plays a crucial role in DNA replication by synthesizing RNA primers for Okazaki fragments. It is closely associated with age at natural menopause, early menopause and POI in European women. In this study, we aimed to investigate whether mutations in PRIM1 contribute to POI in Chinese women. All exons and exon-intron boundaries of PRIM1 gene were sequenced in 192 Han Chinese women with non-syndromic POI...
August 24, 2016: Reproductive Biomedicine Online
Jigal Haas, Eran Barzilay, Ariel Hourvitz, Jehoshua Dor, Shlomo Lipitz, Yoav Yinon, Mor Shlomi, Adrian Shulman
Multifetal pregnancy reduction (MPR) is an accepted method of reducing complications of triplet pregnancies and higher-order multifetal pregnancies. Eighty-three pregnancies that underwent early (68 weeks) transvaginal MPR were compared with 125 pregnancies that underwent late (11-14 weeks) transabdominal MPR. Rates of pregnancy loss, preterm delivery, gestational diabetes and hypertensive disorders were similar among both groups. Early MPR was associated with a lower risk for small for gestational age newborns (6...
August 24, 2016: Reproductive Biomedicine Online
Juan A Garcia-Velasco, Belen Acevedo, Claudio Alvarez, Monica Alvarez, Jose Bellver, Juan Fontes, Jose Landeras, Dolors Manau, Francisca Martinez, Elkin Muñoz, Ana Robles, Luis Rodriguez-Tabernero
No abstract text is available yet for this article.
August 24, 2016: Reproductive Biomedicine Online
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