Read by QxMD icon Read

Journal of Thrombosis and Haemostasis: JTH

Cédric Garcia, Nicolas Montée, Julien Faccini, Jennifer Series, Olivier Meilhac, Anne-Valérie Cantero, Pauline Le Faouder, Meyer Elbaz, Bernard Payrastre, Cécile Vindis
BACKGROUND: While high-density lipoproteins (HDL) have anti-thrombotic effects by reducing platelet activation, the relationship between HDL levels and the risk of acute coronary syndrome (ACS) is unclear since HDL particles are heterogeneous in composition and biological properties OBJECTIVE: We characterized the effects of HDL2 and HDL3 subclasses from ACS patients and non-coronary artery disease (CAD) subjects on platelet activation. METHODS: We measured platelet aggregation and ex vivo thrombus formation, analyzed signaling pathways by flow cytometry and performed a targeted lipidomics analysis on HDL subclasses RESULTS: Analysis of human platelet aggregation in suspension, adhesion on von Willebrand factor and thrombus formation on collagen under arterial shear demonstrated that HDL2 subclass from ACS patients had a higher antiplatelet potency compared to ACS HDL3 particles and HDL subclasses from non-CAD subjects...
March 15, 2018: Journal of Thrombosis and Haemostasis: JTH
Sophie Testa, Oriana Paoletti, Cristina Legnani, Claudia Dellanoce, Emilia Antonucci, Benilde Cosmi, Vittorio Pengo, Daniela Poli, Rossella Morandini, Roberto Testa, Armando Tripodi, Gualtiero Palareti
BACKGROUND: Direct oral anticoagulants(DOACs) are administered at fixed dose without need for dose adjustment by lab testing. A high inter-individual variability in the drug blood levels was shown with all DOACs. To evaluate a possible relationship between DOAC C-trough anticoagulant levels and thromboembolic events,565 consecutivenaïve patients withatrial fibrillation (AF), were enrolled in this study performed within the START-Laboratory Registry. METHODS: DOAC specific measurements [diluted thrombin time (dTT) or anti-FIIa calibrated for dabigatran; anti-FXa calibrated for rivaroxaban or apixaban] at C-trough were performed locally at steady state within 15-25 days from starting treatment...
March 12, 2018: Journal of Thrombosis and Haemostasis: JTH
Cecilia Augustsson, Anders Svensson, Birgitte Kjaer, Tzu-Yuan Chao, Xia Wenjuan, Berit Olsen Krogh, Jens Breinholt, Jes Thorn Clausen, Ida Hilden, Helle Heibroch Petersen, Lars Christian Petersen
BACKGROUND: Initiation of coagulation is induced by binding of activated factor VII (FVIIa) to tissue factor (TF) and activation of factor X (FX) in a process regulated by tissue factor pathway inhibitor (TFPI). TFPI contains three Kunitz-type protease inhibitor domains (K1-K3) of which K1 and K2 block the active sites of FVIIa and FXa respectively. OBJECTIVE: To produce a monoclonal antibody (mAb) directed towards K1, to characterize the binding epitope, and to study its effect on TFPI inhibition...
March 12, 2018: Journal of Thrombosis and Haemostasis: JTH
Jonathan H Foley, Edward M Conway
The complement system, comprising over 30 soluble and cell-based proteins, is a major component of innate immunity, providing mechanisms of surveillance to detect and discard invading pathogens and damaged host cells (reviewed in [1, 2]). Triggered by recognition of altered molecular complexes on membrane surfaces that are sensed as danger signals, complement activation proceeds via three cascade-like pathways - lectin, classical, and alternative - which converge with the formation of a C3 convertase. Subsequent downstream generation of multiple complement byproducts, including the anaphylatoxins, C3a and C5a, leads to inflammation, opsonization, phagocytosis, and destruction of the target cell/pathogen, with recruitment of an adaptive immune response to effect long-term protection and repair...
March 8, 2018: Journal of Thrombosis and Haemostasis: JTH
C Helten, D Naguib, L Dannenberg, M Pöhl, A Ayhan, T Hohlfeld, B Levkau, M Kelm, T Zeus, A Polzin
BACKGROUND: High on-treatment platelet reactivity (HTPR) to antiplatelet medication leads to ischemic events, while low on-treatment platelet reactivity (LTPR) increases bleeding risk. However, various trials failed to demonstrate superiority of tailored antiplatelet regimes (ARCTIC, ANTARCTIC, Trigger-PCI, GRAVITAS). TROPICAL-ACS was the first study that proved benefit of tailoring antiplatelet medication according to platelet function analyzing. A potential reason may be that different platelet function assays were used in these trials...
March 7, 2018: Journal of Thrombosis and Haemostasis: JTH
P Sasikumar, K S AlOuda, W J Kaiser, L M Holbrook, N Kriek, J A Unsworth, A P Bye, T Sage, R Ushioda, K Nagata, R W Farndale, J M Gibbins
OBJECTIVE: Heat shock protein 47 (HSP47) is an intracellular chaperone protein that is vital for collagen biosynthesis in collagen secreting cells. This protein has also been shown to be present on the surface of platelets. Given the importance of collagen and its interactions with platelets in triggering haemostasis and thrombosis, in this study we sought to characterise the role of HSP47 on these cells. APPROACH AND RESULTS: The deletion of HSP47 in mouse platelets or its inhibition in human platelets reduced their function in response to collagen and the GPVI agonist (CRP-XL), but responses to thrombin were unaltered...
March 7, 2018: Journal of Thrombosis and Haemostasis: JTH
Hansjorg Schwertz, Matthew T Rondina
We appreciate the comments from de la Salle and colleagues regarding our recent findings on all-trans retinoic acid (atRA) as a translational control mechanism in human platelets[1]. We agree with the notion that when performing platelet molecular biology studies, highly-purified platelet preparations are needed. As described in this report[1] and others[2-6], we utilized a rigorous and validated method in the field of purifying platelets from whole blood[7-10]. In this method, leukocyte-immunodepleted suspensions are prepared using anti-CD45 magnetic beads...
March 6, 2018: Journal of Thrombosis and Haemostasis: JTH
Maria Farm, Anwar J Siddiqui, Liselotte Onelöv, Ingela Järnberg, Jaak Eintrei, Filip Maskovic, Anders Kallner, Margareta Holmström, Jovan P Antovic
BACKGROUND: The study compares different D-dimer assays and age-adjusted cutoffs in outpatients with suspected venous thromboembolism (VTE). The plasma concentration of this sensitive biomarker is increased by activated coagulation, but also by several conditions that are linked to an increased risk of VTE. One such condition is old age, which poses a common clinical problem where many prefer not to analyze D-dimer in elderly patients. Age-adjusted cutoffs have been validated for both deep venous thrombosis (DVT) and pulmonary embolism, aiming to increase specificity without notably decreasing sensitivity...
March 5, 2018: Journal of Thrombosis and Haemostasis: JTH
H de la Salle, C Angénieux, F Lanza, C Gachet
In a recent report published in J Thromb Haemost, Schwertz et al. [1] investigated whether all-trans retinoic acid (atRA) controls mRNA translation in human platelets. This report concluded that atRA negatively controls the interaction between retinoic acid receptor alpha protein (RARα) and specific mRNAs, thereby positively regulating their translation in platelets. These interesting observations could open a new field in the understanding of platelet biology and we would like to discuss four important aspects of this work that deserve to be considered in this perspective...
March 5, 2018: Journal of Thrombosis and Haemostasis: JTH
L B Harrington, M Blondon, M Cushman, A M Kaunitz, M A Allison, L Wang, S Sullivan, N F Woods, A Z LaCroix, S R Heckbert, B McKnight, J Rossouw, N L Smith
BACKGROUND: Vasomotor symptoms may be markers of changes in cardiovascular risk, but it is unknown whether these symptoms are associated with the risk of venous thrombosis (VT). OBJECTIVE: To evaluate the association of vasomotor symptom presence and severity with incident VT risk among postmenopausal women, independent of potential explanatory variables. METHODS: This cohort study included participants of the Women's Health Initiative (WHI) Hormone Therapy Trials (n=24,508) and Observational Study (n=87,783), analyzed separately...
March 5, 2018: Journal of Thrombosis and Haemostasis: JTH
Lindsey A Miles, Nagyung Baik, Hongdong Bai, Helen P Makarenkova, William B Kiosses, Stan Krajewski, Francis J Castellino, Alex Valenzuela, Nissi M Varki, Barbara M Mueller, Robert J Parmer
BACKGROUND: Lactational competence requires plasminogen, the zymogen of the serine protease, plasmin. Plg-RKT is a unique transmembrane plasminogen receptor that promotes plasminogen activation to plasmin on cell surfaces. Plg-RKT -/- mice are viable, but no offspring of Plg-RKT -/- female mice survive to weaning. OBJECTIVES: We investigated potential lactational failure in Plg-RKT -/- mice and addressed causal mechanisms. METHODS: Fibrin accumulation, macrophage infiltration, processing of extracellular matrix components, effects of genetic deletion of fibrinogen, expression of fibrosis genes, and proliferation and apoptosis of epithelial cells were examined in lactating mammary glands of Plg-RKT -/- and Plg-RKT +/+ mice...
March 1, 2018: Journal of Thrombosis and Haemostasis: JTH
Mehran Mirramezani, Bradley A Herbig, Timothy J Stalker, Leonard Nettey, Matthew Cooper, John W Weisel, Scott L Diamond, Talid Sinno, Lawrence F Brass, Shawn C Shadden, Maurizio Tomaiuolo
BACKGROUND: Hemostasis studies performed in vivo have shown that hemostatic plugs formed after penetrating injuries are characterized by a core of highly-activated densely-packed platelets near the injury site, covered by a shell of less-activated and loosely-packed platelets. Thrombin production occurs near the injury site, further activating platelets and starting the process of platelet mass retraction. Tightening of interplatelet gaps may then prevent the escape and exchange of solutes...
February 28, 2018: Journal of Thrombosis and Haemostasis: JTH
Tomoyuki Sasaki, Toshiaki Shirai, Nagaharu Tsukiji, Shimon Otake, Shogo Tamura, Jiro Ichikawa, Makoto Osada, Kaneo Satoh, Yukio Ozaki, Katsue Suzuki-Inoue
BACKGROUND: Rhodocytin, a disulfide-linked heterodimeric C-type lectin consisting of α- and β-subunits from Calloselasma rhodostoma, induces platelet aggregation through C-type lectin-like receptor 2 (CLEC-2). CLEC-2 is a physiological binding partner of podoplanin (PDPN) expressed on some tumor cell types and is involved in tumor cell-induced platelet aggregation and tumor metastasis. Thus, modified rhodocytin may be a possible source for anti-CLEC-2 medicine in both anti-platelet and anti-metastasis therapy...
February 28, 2018: Journal of Thrombosis and Haemostasis: JTH
L Muszbek, K Pénzes, É Katona
Acquired FXIII deficiencies caused by autoantibodies against FXIII subunits represent rare but very severe bleeding diatheses. Alloantibodies in FXIII deficient patients also cause life-threatening bleeding complication, but they develop extremely rarely. In this review we provide an overview on the diagnosis and classification of anti-FXIII antibodies and analyze 48 patients with autoimmune FXIII deficiency and 4 additional FXIII deficient patients who developed anti-FXIII alloantibody. The patients were collected from peer-reviewed publications from which relevant data could be extracted...
February 20, 2018: Journal of Thrombosis and Haemostasis: JTH
T E van Mens, L M van der Pol, N van Es, I M Bistervels, A T A Mairuhu, T van der Hulle, F A Klok, M V Huisman, S Middeldorp
BACKGROUND: In patients suspected of pulmonary embolism (PE), clinical decision rules are combined with D-dimer testing to rule out PE, avoiding the need for imaging in those at low risk. Despite sex-differences in several disease aspects including its diagnosis, these algorithms are used indiscriminately in women and men. OBJECTIVES: To compare the performance, defined as efficiency and failure rate, of three pre-imaging diagnostic algorithms for PE between women and men: the Wells rule with fixed or with age adjusted D-dimer cutoff, and a recently validated algorithm (YEARS)...
February 20, 2018: Journal of Thrombosis and Haemostasis: JTH
Roshni Kulkarni, Andra H James, Miranda Norton, Amy Shapiro
BACKGROUND: A high-purity, plasma-derived factor X concentrate (pdFX) is approved to treat hereditary factor X deficiency, an autosomal recessive disorder. OBJECTIVE: Post hoc assessment of pdFX pharmacokinetics, safety, and efficacy in women and girls with hereditary factor X deficiency. PATIENTS/METHODS: Subjects aged ≥12 years with moderate/severe factor X deficiency (plasma factor X activity <5 IU/dL) received on-demand or preventive pdFX (25 IU/kg) for ≤2 years...
February 20, 2018: Journal of Thrombosis and Haemostasis: JTH
R Marlu, T Jouve, B Polack, P Sié, V Mémier
We read with great interest the recent article by Jaffer et al. (1) comparing the ecarin chromogenic assay (ECA) and the diluted thrombin time (dTT) with high-performance liquid chromatography mass spectrometry assay (HPLC/MS) for the quantification of dabigatran concentrations in plasma. In this article, the ECA was performed using a STA-ECA-II kit with STA dabigatran calibrators (Diagnostica Stago, Asnières sur Seine, France) and dTT was performed using the Hemoclot Thrombin Inhibitor assay (HTI) (Hyphen BioMed, Neuville-sur-Oise, France)...
February 20, 2018: Journal of Thrombosis and Haemostasis: JTH
Cecilia Becattini, Michela Giustozzi, Maria Giovanna Ranalli, Giulio Bogliari, Federica Cianella, Melina Verso, Giancarlo Agnelli, Maria Cristina Vedovati
BACKGROUND: Chronic kidney disease is a risk factor for major bleeding in patients with atrial fibrillation (AF) treated with warfarin. OBJECTIVE: To assess the effect of variations of renal function over time on the risk for major bleeding (MB) during treatment with direct anticoagulants (DOACs) in patients with non-valvular AF. METHODS: Consecutive AF patients were prospectively followed since they received the first DOACs prescription. Estimated glomerular filtration rate (eGFR) was periodically assessed and the incidence of MB recorded...
February 20, 2018: Journal of Thrombosis and Haemostasis: JTH
Kirsteen R Burton, Alison L Park, Michael Fralick, Joel G Ray
BACKGROUND: The risk of breast cancer may be higher with direct exposure to ionizing radiation from thoracic computed tomography (CT) during pregnancy or postpartum. We evaluated the short-term risk of maternal breast cancer after exposure to thoracic CT during these periods. METHODS: We completed a retrospective population-based cohort study of all deliveries between 1995-2014 using universal health care databases in the province of Ontario, Canada. The main exposure was thoracic CT in pregnancy or ≤ 42 days postpartum...
February 16, 2018: Journal of Thrombosis and Haemostasis: JTH
K M J Devreese, T L Ortel, V Pengo, B de Laat
No abstract text is available yet for this article.
February 14, 2018: Journal of Thrombosis and Haemostasis: JTH
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"