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Human Pathology

Juliana Machado-Rugolo, Alexandre Todorovic Fabro, Daniel Ascheri, Cecília Farhat, Alexandre Muxfeldt Ab'Saber, Vanessa Karen de Sá, Maria Aparecida Nagai, Teresa Takagaki, Ricardo Terra, Edwin Roger Parra, Vera Luiza Capelozzi
To demonstrate the usefulness of complementary next-generation sequencing (NGS) and immunohistochemistry (IHC) counting, we analyzed 196 patients with non-small cell lung cancer (NSCLC) who underwent surgical resection and adjuvant chemotherapy. Formalin-fixed paraffin-embedded samples of adenocarcinoma (ADC), squamous cell carcinoma (SqCC), and large-cell carcinoma (LCC) were used to prepare tissue microarrays and were examined by protein H-score IHC image analysis and NGS for oncogenes and proto-oncogenes and genes of tumor suppressors, immune checkpoints, epithelial-mesenchymal transition factors, tyrosine kinase receptors, and vascular endothelial growth factors (VEGFs)...
September 12, 2018: Human Pathology
Chunyi Jiang, Yi Zhou, Yiyang Huang, Yan Wang, Wei Wang, Xiaoling Kuai
ADAMTS-2 is a member of the ADAMTS family and is a procollagen N-proteinase. The objective of our research is to explore the prognostic significance of ADAMTS-2 in gastric carcinoma. A total of 655 samples with full clinicopathological data were investigated in this study. Tissue microarray immunohistochemistry (TMA-IHC) analysis was used to analyze the relationship between clinicopathological characteristics and ADAMTS-2 expression. Oncomine and Kaplan-Meier plotters were performed for the relationship analysis between prognosis and ADAMTS-2 expression in patients with gastric cancer (GC)...
September 12, 2018: Human Pathology
Wei Wang, Guangyao Song, Jie Lin, Xingzheng Zheng, Yuxiang Wang, Yan Liu, Congrong Liu
As a new pattern-based classification, the Silva pattern system has been recently developed to evaluate invasive lymph node (LN) metastasis and the prognosis of endocervical adenocarcinoma (EAC). Therefore, our study was conducted to explore the reproducibility and prognostic significance of this system in a multi-institutional Chinese cohort, with the goal of revising and expanding its application. The clinicopathological data of 191 EAC patients from 3 medical centers were examined in a retrospective manner...
September 12, 2018: Human Pathology
Jumei Liu, Li Liang, Sixia Huang, Lin Nong, Dong Li, Bo Zhang, Ting Li
Enhancer of zeste homolog 2 (EZH2), a H3K27-specific histone methyltransferase, has been shown to be frequently overexpressed in various human cancers including lymphoma. Here we investigate the expression and functionality of EZH2 and H3K27me3 in extranodal NK/T-cell lymphoma, nasal type (ENKTL). Results of NanoString analysis revealed that EZH2 and related histone H3 families were upregulated genes in ENKTL tissues. Results of immunohistochemistry (IHC) demonstrated that EZH2 and trimethylation of Lys-27 in histone (H3K27me3) were highly expressed in 55...
September 12, 2018: Human Pathology
Neda Kalhor, Cesar A Moran
Seven primary thymic epithelial neoplasms, three thymomas, and four thymic carcinomas with rhabdomyomatous component are herein described. The patients are two women and 5 men between the ages of 42 and 62years (average: 52years). Clinically, the patients presented with non-specific symptoms of cough, chest pain, and dyspnea. None of the patients had history of myasthenia gravis or of previous malignancy. Diagnostic imaging revealed the presence of an anterior mediastinal mass in all the patients. Surgical resection was accomplished in all the cases...
September 11, 2018: Human Pathology
Andrianos Tsekrekos, Sönke Detlefsen, Robert Riddell, James Conner, Luca Mastracci, Kieran Sheahan, Jayant Shetye, Lars Lundell, Michael Vieth
Studies investigating the histopathologic response of gastric carcinoma to neoadjuvant treatment have used a variety of different tumor regression grading systems. The aim of this Delphi survey was to review the available systems and reach consensus on a potential international standard. An international email-based Delphi survey involving six expert pathologists was undertaken between January and October 2017. A questionnaire consisting of seventy-two items was formed after reviewing the five available systems...
September 11, 2018: Human Pathology
Jennifer B Gordetsky, David Ullman, Luciana Schultz, Kristin K Porter, Maria Del Carmen Rodriguez Pena, Carli E Calderone, Jeffrey W Nix, Michael Ullman, Sejong Bae, Soroush Rais-Bahrami
Magnetic Resonance Imaging (MRI)/Ultrasound fusion-targeted biopsy (TB) has been shown to more accurately identify higher grade prostate cancers compared to standard-of-care systematic sextant prostate biopsy (SB). However, occasional false positive imaging findings occur. We investigated the histologic findings associated with false positive prostate MRI findings. A retrospective review was performed on our surgical pathology database from 2014-2017 selecting patients with no cancer detected on TB with concurrent SB following at least one prior benign SB session...
September 1, 2018: Human Pathology
Yuqing Cheng, Mingzhan Du, Yaohui Wang, Tianyun Liu, Xiaoli Zhou, Ling Chen, Xiangshan Fan, Lingchuan Guo, Yifen Zhang, Dan Zhou, Qin Huang
Clinicopathology and risk factors of lymph node metastasis (LNM) in micropapillary early (pT1) gastric carcinoma (MEGC) remain elusive because of the extreme rarity. In this multicenter study, we investigated 1890 consecutive radical resections of early gastric carcinoma diagnosed with the World Health Organization criteria and identified 29 (1.5%) MEGC cases with a small (≥ 5%) micropapillary component. MEGC showed a male predominance (M:F=21:8). Most (93.1%, 27/29) tumors invaded submucosa. Lymphovascular invasion was detected in 14 (48...
September 1, 2018: Human Pathology
Roberto Herrera-Goepfert, Giovanny Soca-Chafre, Samuel Almeida-Navarro, Angélica Montiel-Dávalos
Adenocarcinomas of the ampulla of Vater account for 0.5% of malignant neoplasms of the gastrointestinal tract, and 6-20% of malignant periampullary neoplasms, the majority of patients being candidates to elective surgery. Our objective was to evaluate the clinicopathological prognostic factors of ampullary adenocarcinomas after surgical resection, in the Mexican population. From the records of the Department of Pathology at the Instituto Nacional de Cancerología, México, cases diagnosed as adenocarcinomas of the ampulla of Vater were selected over a period of 11years, from January 2005 to September 2015...
September 1, 2018: Human Pathology
Meghan Hupp, Sarah Williams, Brian Dunnette, Katelyn M Tessier, Elizabeth L Courville
Incorporation of a MYC immunohistochemical stain in the work-up of large B-cell lymphomas has become common in hematopathology practice. Evaluation of this stain can be difficult due to staining heterogeneity and can have inter-observer variability, particularly when performed on entire tumor sections. We identified 87 cases of aggressive B cell lymphoma (34 core needle and 53 excisional biopsies) and compared the following methods of MYC immunohistochemical staining evaluation: the original pathologist's interpretation, a systematic retrospective method of evaluation by manual analysis, and a retrospective method of evaluation by digital image analysis (using scanned slides analyzed via the Aperio Nuclear algorithm)...
August 30, 2018: Human Pathology
Xinming Chen, Weiwei Shao, Hua Huang, Xiaochun Feng, Sumei Yao, Honggang Ke
We investigated the expression of Reticulocalbin-1 (RCN1) and their prognostic significance in non-small cell lung cancer (NSCLC). RCN1 expression was evaluated by immunohistochemical analysis with tissue microarrays in NSCLC tissues and matched adjacent non-cancerous tissues. Further, quantitative polymerase chain reaction (qPCR) and western blot were also employed to examine the expression of RCN1. Moreover, the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), clone formation and transwell assays were used to measure cell proliferation, migration and invasion...
August 30, 2018: Human Pathology
Vatsal Patel, Benjamin Kipp, J Kenneth Schoolmeester
Mesonephric-like adenocarcinoma is a recently described adenocarcinoma of the uterine body and ovary with overlapping features of mesonephric adenocarcinoma and endometrioid carcinoma. It is thought to be a mullerian adenocarcinoma that has differentiated along mesonephric lines. A 71-year-old woman had a 3cm endometrial mass that invaded myometrium without gross or microscopic evidence of cervical involvement. The tumor had a variety of architectural patterns and produced prominent stromal hyalinization containing embedded cords and trabeculae of tumor cells...
August 30, 2018: Human Pathology
Gabriel Acosta-Gonzalez, Madhu Ouseph, Kara Lombardo, Shaolei Lu, Jonathan Glickman, Murray Resnick
The serrated neoplasia pathway accounts for approximately 20% of colorectal carcinomas (CRCs). Sessile serrated adenomas (SSAs), the main precursor lesion of the serrated pathway, are molecularly driven by MLH1 promoter methylation and microsatellite instability (MSI) in their progression to CRC. MSI-high (MSI-H) lesions are highly immunogenic and associated with a high density of tumor infiltrating lymphocytes. Our study's aim was to determine how the kinetics of this immune environment relates to SSAs in their progression through low-grade (SSA-LD) to high-grade dysplasia (SSA-HD), and CRC...
August 30, 2018: Human Pathology
Huiya Huang, Jason Chang, Stefano Rosati, Jennifer Geurts, A Craig Mackinnon
Patients with Familial Adenomatous Polyposis (FAP) have a higher incidence for developing adrenal neoplasms, most of which are non-functioning with conventional histologic appearance. We report a patient with a history of multiple colon polyps who developed an adrenocortical adenoma with unusual morphology. The tumor showed a tubulo-papillary architecture and plasmacytoid cytomorphology that were distinct from conventional adrenocortical adenomas. β-catenin stain showed aberrant nuclear positivity in the tumor, suggesting an altered β-catenin related pathway...
August 30, 2018: Human Pathology
Rei Atono Itou, Naoki Uyama, Seiichi Hirota, Norifumi Kawada, Songtao Wu, Seikan Miyashita, Ikuo Nakamura, Kazuhiro Suzumura, Hideaki Sueoka, Tosihiro Okada, Etsuro Hatano, Hiroko Tsutsui, Jiro Fujimoto
Cancer-associated fibroblasts (CAFs) are an important constituent of the cancer stroma. In intrahepatic cholangiocarcinoma (ICC), the features of CAFs at the primary site and in the metastatic lymph nodes (Met-LNs) and their origin have been unclear. In the present study, we characterized CAFs at the primary site (n=42) and in the Met-LNs (n=10) of human ICC by immunohistochemistry using potential molecular markers of CAFs, portal fibroblasts (PFs), hepatic stellate cells (HSCs) and bone marrow-derived fibrocytes (BMDFs)...
August 30, 2018: Human Pathology
Georgia Karpathiou, Xavier Matias-Guiu, Mousa Mobarki, Charlotte Vermesch, Marie-Laure Stachowicz, Celine Chauleur, Michel Peoc'h
Neuroendocrine tumors of the ovary are rare and of uncertain histogenesis. They may be primary or metastatic. Pathogenesis of ovarian carcinomas remains unknown. We report the case of an ovarian large cell carcinoma expressing all neuroendocrine markers (CD56, Chromogranin A, Synaptophysin) that presented as a primary tumor and coexisted with a typical endometrial serous carcinoma also expressing one neuroendocrine marker (CD56). The two tumors had identical molecular mutational profiles, as examined by Next Generation Sequencing...
August 29, 2018: Human Pathology
Tingting Bian, Daishan Jiang, Jia Feng, Jian Liu, Li Qian, Qing Zhang, Xiaoli Li, Yifei Liu, Jianguo Zhang
Previous studies have proven that the lepidic component of lung adenocarcinoma is an independent prognostic factor and has a favorable effect on patient prognosis; however, no studies have reported the specific distribution of the lepidic component in lung cancer. In this study, we focused mainly on whether the lepidic component at the tumor margin was an independent prognostic factor for invasive lung adenocarcinoma. We reviewed 276 patients with invasive lung adenocarcinomas and divided them into two groups: 181 with tumors ≤3cm and 95 with tumors >3cm to study their histopathologic and clinicopathologic characteristics...
August 29, 2018: Human Pathology
Min Hu, B D Tingkuan Zhao, Jing Liu, Zou Zhiming, B D Qing Xu, B D Ping Gong, Huaxiong Guo
Liver kinase B1 (LKB1) is a newly discovered tumor suppressor gene that plays a role in tumorigenesis and cancer progression. However, LKB1 expression and its precise impact on gastric cancer (GC) have not yet been elucidated. The aim of this study was to explore the significance of LKB1 expression, as well as its correlation with epithelial-mesenchymal transition (EMT) in GC. In the present study, LKB1 protein was detected in 107 GC tissue samples and adjacent paracancerous tissues by immunohistochemical (IHC) staining...
August 29, 2018: Human Pathology
Jianzhong Cao, Penghui Wang, Jiemin Chen, Xiaodong He
Phosphatidylinositol glycan anchor biosynthesis class U (PIGU), which is a critical subunit of the glycosylphosphatidylinositol transamidase (GPI-T) complex, has been reported to be an oncogene in bladder cancer. However, the expression and prognostic significance of PIGU in hepatocellular carcinoma (HCC) remain unclear. In this study, we conducted bioinformatics, quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC) analysis to investigate the expression profile of GPI-T subunits in HCC tissues, finding that PIGU was the most significantly overexpressed GPI-T subunit in HCC tissues at both the RNA and protein levels...
August 29, 2018: Human Pathology
William Selove, Jennifer Picarsic, Steven H Swerdlow
Although littoral cell angiomas (LCA) are phenotypically well-characterized, the antibodies used to support the diagnosis identify many other cells in the normal spleen and some may be found in other angiomatous lesions. Based on a langerin/CD207+ LCA index case, langerin and other selected immunohistochemical (IHC) staining was performed on 10 LCA, 20 other splenic angiomatous lesions and 7 reactive lymph nodes to further investigate the role of langerin as a diagnostic tool. 90% of LCA (9/10) were langerin positive, while only 1/20 other splenic vascular lesions was partially positive (5%) (P < ...
August 18, 2018: Human Pathology
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