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Mark Bettington, Christophe Rosty, Vicki Whitehall, Barbara Leggett, Diane McKeone, Sally-Ann Pearson, Neal Walker
AIMS: The traditional serrated adenoma (TSA) is the least common subtype of serrated colorectal polyp. Large protuberant lesions are easily recognized, however, the origins of TSAs are not known and early forms have not been described. Some large TSAs present with a flat "shoulder" component surrounding the central protuberant component. We hypothesized that small polyps with the same histology as these "shoulder" regions may represent early TSAs. METHODS AND RESULTS: We collected 70 small (< 10mm) polyps that may represent early TSAs based on typical TSA cytology covering the luminal surface...
July 14, 2018: Histopathology
Silvia Darb-Esfahani, Ivonne Kolaschinski, Fabian Trillsch, Sven Mahner, Nicole Concin, Ignace Vergote, Els Van Nieuwenhuysen, Patriciu Achimas-Cadariu, Joanna Glajzer, Hannah Woopen, Stephan Wienert, Eliane Tabea Taube, Mandy Stanske, Hagen Kulbe, Carsten Denkert, Jalid Sehouli, Elena Ioana Braicu
OBJECTIVE: Advanced-stage ovarian high-grade serous carcinoma (HGSC) is a poor-prognosis cancer, however a small and poorly characterized subset of patients shows long-term survival. We aimed to establish a cohort of HGSC long-term survivors for histopathological and molecular analysis. METHODS: Paraffin blocks from 151 patients with primary FIGO III/IV HGSC and progression-free survival (PFS) >5 years were collected within the Tumorbank Ovarian Cancer (TOC) Network...
July 14, 2018: Histopathology
Esther Campos-Fernández, Flavia Sayuri Matsuo, Marília Ferreira Andrade, João Paulo Silva Servato, Adriano Mota Loyola, Sérgio Vitorino Cardoso, Sindeval José Siva, Alberto da Silva Moraes, Paulo Rogério de Faria
AIMS: Studies about epigenetics in oral squamous cell carcinoma (OSCC) are rare. Histone modifications comprise epigenetic mechanisms that perform a key role in gene transcription and may regulate tumour development. Thus, we asked whether the following post-translational histone modifications, H3S10 phosphorylation (H3S10ph) and H4K12 acetylation (H4K12ac) had prognostic value for OSCC patients. METHODS AND RESULTS: Paraffin-embedded tissue samples of 90 patients diagnosed with OSCC were raised and submitted to immunohistochemical staining with antibodies against H3S10ph and H4K12ac...
July 14, 2018: Histopathology
Joep J de Jong, Hans Stoop, Daan Nieboer, Joost L Boormans, Geert J L H van Leenders
AIMS: Programmed Death-Ligand (PD-L1) expression has predictive value for response to immune-checkpoint inhibitor treatment in urothelial cancer patients. The consistency of PD-L1 expression amongst different specimen types is however unknown. The aim of the study is to compare PD-L1 expression in matched transurethral resections of the bladder (TURB), cystectomy specimens, and lymph node metastases of urothelial cancer patients. METHODS AND RESULTS: We performed PD-L1 (SP142) immunohistochemistry on whole tissue slides of 115 urothelial carcinoma patients who had undergone TURB, followed by radical cystectomy and/or pelvic lymph node dissection...
July 12, 2018: Histopathology
Ezra Baraban, Paul J Zhang, Shabnam Jaffer, Daniel Lubin, Michael Feldman, Ira J Bleiweiss, Anupma Nayak
AIMS: Adenomyoepithelioma (AME) and adenoid cystic carcinoma (ACC) of the breast have been noted to occur simultaneously, raising the possibility that AME may represent a related or precursor lesion to ACC. ACC frequently harbors genetic rearrangement of the MYB gene. We sought to clarify the relationship between AME and ACC by comparing their rates of MYB expression by IHC and MYB rearrangement by FISH. METHODS AND RESULTS: IHC as well as FISH for MYB rearrangement were performed on paraffin embedded sections of 11 breast-ACCs, 11 non-breast ACCs and 11 breast-AMEs...
July 12, 2018: Histopathology
Konstantinos Linos, Laura J Tafe
BACKGROUND: Isocitrate dehydrogenase 1 (IDH1) is a metabolic enzyme that converts isocitrate to α-ketoglutarate. IDH1 mutations are associated with the accumulation of the oncometabolite D-2-hydroxyglutarate, which acts as an epigenetic modifier, and the development of multiple malignancies. METHODS: From May 2013 - June 2017, 252 melanoma samples from 214 patients with advanced or distant metastatic disease were tested for somatic mutations with the 50 gene AmpliSeq v2 Cancer Hotspot Panel...
July 12, 2018: Histopathology
Emad A Rakha, Mohammed A Aleskandarany, Michael S Toss, Nigel Mongan, Maysa E ElSayed, Andrew R Green, Ian O Ellis, Leslie W Dalton
BACKGROUND: Histological grade is an independent prognostic variable in breast cancer (BC). Previous concordance studies of BC grade have reported moderate levels of agreement; a typical finding in morphological assessment of biological variables. This study aims at investigating the impact of discordance on the prognostic value of grade and identifying the best reporting approach in borderline cases. METHODS: A large (n=1675) well-characterised annotated cohort of BC originally graded in routine practice using glass slides was re-graded twice, by an expert breast pathologist using virtual microscopy with a three months washout period...
July 12, 2018: Histopathology
Makoto Urano, Hideaki Hirai, Yuichiro Tada, Daisuke Kawakita, Tomotaka Shimura, Kiyoaki Tsukahara, Satoshi Kano, Hiroyuki Ozawa, Kenji Okami, Yuichiro Sato, Chihiro Fushimi, Akira Shimizu, Soichiro Takase, Takuro Okada, Hiroki Sato, Yorihisa Imanishi, Kuninori Otsuka, Yoshihiro Watanabe, Akihiro Sakai, Koji Ebisumoto, Takafumi Togashi, Yushi Ueki, Hisayuki Ota, Yukiko Sato, Natsuki Saigusa, Masato Nakaguro, Toyoyuki Hanazawa, Toshitaka Nagao
AIMS: Salivary duct carcinoma (SDC) is an uncommon, aggressive tumour that histologically resembles high-grade mammary ductal carcinoma, and it is characterized by the expression of androgen receptor (AR). The androgen signalling pathway, a potential therapeutic target, can be regulated by forkhead-box protein A1 (FOXA1). This study aimed to evaluate the clinicopathological implications of FOXA1 in SDC. METHODS AND RESULTS: We examined the relationship between the immunoexpression of FOXA1 and FOXA1 mutations and clinicopathological factors, including the biomarker status and clinical outcome, in 142 SDCs...
July 11, 2018: Histopathology
Lin-Zhou Zhang, Qi-Wen Man, Jin-Yuan Liu, Wen-Qun Zhong, Yue-Yu Zheng, Yi-Fang Zhao, Bing Liu
AIMS: The purpose of this study was to explore the potential involvement of Fra-1, c-Jun and c-Fos, three vital members of the AP-1 complex, in the pathogenesis of odontogenic keratocysts (OKCs). METHODS AND RESULTS: Tissue samples, containing 10 normal oral mucosa (OM), 10 dentigerous cysts (DC) and 32 OKC specimens, were applied to investigate the expression levels of Fra-1, c-Jun and c-Fos by immunohistochemistry and real-time quantitative polymerase chain reaction (RT-qPCR)...
July 11, 2018: Histopathology
Timco Koopman, Bert van der Vegt, Marcel Dijkstra, Joost Bart, Evelien Duiker, G Bea A Wisman, Geertruida H de Bock, Harry Hollema
AIMS: treatment with anti-HER2-therapy could be beneficial for patients with HER2-positive endometrial and ovarian clear cell carcinoma (CCC). We studied HER2 overexpression by immunohistochemistry (IHC) using three different antibodies, including concordance with amplification by in situ hybridization (ISH). METHODS AND RESULTS: IHC and ISH were performed on tissue microarrays of 101 tumours: 58 endometrial pure CCC, 19 endometrial mixed carcinomas with a CCC component and 24 ovarian pure CCC...
July 10, 2018: Histopathology
Elizabeth G Demicco, Khalida Wani, Davis Ingram, Michael Wagner, Robert G Maki, Anthony Rizzo, Alan Meeker, Alexander J Lazar, Wei-Lien Wang
AIMS: TERT promoter mutations have been reported in 22% of solitary fibrous tumors (SFT) and have been associated with poor outcomes. We performed testing for TERT hotspot mutations in a large series of solitary fibrous tumors in order to confirm this finding and explore clinicopathologic correlates of mutation status. METHODS AND RESULTS: PCR for TERT hotspot mutations C250T and C228T was performed on DNA extracted from 216 SFT, and mutation status correlated with clinicopathological factors including predicted risk for metastasis using a previously published model...
July 9, 2018: Histopathology
Timothy Kwang Yong Tay, Peiyong Guan, Benjamin Nathanael Loke, Nur Diyana Md Nasir, Vikneswari Rajasegaran, Aye Aye Thike, Derrick Lian, Kenneth Tou En Chang, Bin Tean Teh, Cedric Chuan Young Ng, Puay Hoon Tan
AIM: This study aims to examine the molecular genetics of paediatric breast fibroepithelial tumours through targeted sequencing of 50 genes. MATERIALS AND METHODS: Formalin fixed paraffin embedded tissue of fibroepithelial tumours diagnosed in a cohort of patients aged 18 years and below were subjected to next generation sequencing using the Haloplex Target Enrichment System (Agilent). RESULTS: Twenty-five conventional and seventeen juvenile fibroadenomas were studied, with MED12 mutations found in 53...
July 3, 2018: Histopathology
Lu Cao, Congying Li, Cong Xu, Guomin Xiang, Fang Liu, Xiaozhen Liu, Jiao Jiao, Shuhua Lv, Yun Niu
AIMS: The mechanism of androgen receptor (AR) promoting tumour growth in ER- breast cancer (BC) is undetermined. Prostate-derived ETS factor (PDEF) is highly restricted to the hormone-regulated tissues of epithelial cells, such as those in the prostate, breast and other tissues. It has been demonstrated that PDEF expression is associated with AR in prostate cancer. In this research, we aimed to investigate the relationship between PDEF and AR in ER- BC. METHODS AND RESULTS: We immunohistochemically evaluated the correlation between PDEF and AR expression in 246 cases of ER- invasive BC, and investigated their relationship in ER- BC cell lines...
July 3, 2018: Histopathology
Hyun Jung Lee, Dong Hoon Shin, So Young Kim, Chung Su Hwang, Jung Hee Lee, Won Young Park, Kyung Un Choi, Jee Yeon Kim, Chang Hun Lee, Mee Young Sol, Seo Hee Rha, Sung Woo Park
AIM: Since Xp11.2 translocation-associated renal cell carcinoma (TRCC) was first recognized, its morphological features, and the clinical significance of TFE3 expression, frequency of gene translocation, and diagnostic criteria have been the subject of limited studies. The present retrospective analysis aimed to evaluate the correlation between TFE3 immunoreactivity and its gene translocation status using fluorescence in situ hybridization (FISH) and determine how TFE3 translocation and expression differently affect patient prognosis...
July 2, 2018: Histopathology
Benedikt Martin, Eva Schäfer, Elzbieta Jakubowicz, Patrick Mayr, Regina Ihringer, Matthias Anthuber, Gerhard Schenkirsch, Tina Schaller, Bruno Märkl
AIMS: Tumor budding is considered to be a good marker for progression and prognosis in colorectal carcinomas. A uniform classification system has been established recently. The natural element of uncertainty in the practice of human medicine is also exhibited in the assessment of tumor budding. We tested the hypothesis that the interobserver variability can already be estimated during the assessment process and investigated its potential clinical implication. METHODS AND RESULTS: Six investigators with different levels of experience could perceive different levels of difficulty (LOD) and estimated different levels of interobserver variability (LOIV) (Li1: lower than average; Li2: average; Li3: higher than average) during the assessment of tumor budding in 244 cases of colon cancer (pT3/4)...
June 29, 2018: Histopathology
Véronique Meignin, Françoise Thivolet-Bejui, Marianne Kambouchner, Claire Hussenet, Louise Bondeelle, Andrew Mitchell, Karine Chagnon, Hugues Begueret, Valérie Segers, Vincent Cottin, Abdellatif Tazi, Sylvie Chevret, Claire Danel, Anne Bergeron
Noninfectious pulmonary complications (NIPCs) frequently occur following allogeneic hematopoietic stem cell transplantation (HSCT). As there is no consensus on the description of the related pulmonary pathological lesions, pathologist reports and clinical conclusions are largely inconsistent in routine practice. The aim of our study was to provide an accurate overview of post allogeneic HSCT NIPCs from a large number of lung biopsies. We reviewed 61 lung biopsies in patients with an NIPC, including 51 surgical lung biopsies, 4 post mortem biopsies and 6 lung explants...
June 28, 2018: Histopathology
Anjelica Hodgson, Stanley K Liu, Danny Vesprini, Bin Xu, Michelle R Downes
AIMS: Basal and luminal molecular subgroups of muscle invasive urothelial carcinoma (UC) can be recognized using immunohistochemical markers. Studies have shown that responses to chemotherapy and outcomes differ amongst these subtypes. High grade UC of the bladder is an immunogenic neoplasm which induces a significant amount of intra- and peri-tumoural immune response; the phenotype of infiltrating immune cells may yield prognostic information and predict response to therapy. In this study, we aimed to correlate immunohistochemical phenotype of high grade UC with immune microenvironment composition...
June 27, 2018: Histopathology
Maryam Althobiti, Mohammed A Aleskandarany, Chitra Joseph, Michael Toss, Nigel Mongan, Maria Diez-Rodriguez, Christopher C Nolan, Ibraheem Ashankyty, Ian O Ellis, Andrew R Green, Emad A Rakha
BACKGROUND: Tumour-infiltrating lymphocytes (TILs) in breast cancer (BC) confer prognostic and predictive information. This study aims to assess the spatial and temporal heterogeneity of TILs in BC and its relationship with immune cell subtypes. METHOD: Immunohistochemically-defined immune cell subtypes; T-cell markers (CD3, CD8, and FOXP3), B-cell marker (CD20) and histiocytic marker (CD68) were evaluated in a large series (n=1,165) of invasive BC. A subset of full-face haematoxylin and eosin (H&E) stained slides were examined for TILs heterogeneity within primary tumours and the corresponding local recurrent carcinomas to report on spatial and temporal TILs heterogeneity...
June 27, 2018: Histopathology
Maurizio Puccetti, Sara Ravaioli, Maria Maddalena Tumedei, Elisa Bucchi, Manuela Malmesi, Laura Medri, Sara Bravaccini
BACKGROUND: The diagnosis of breast cancer (BC) is based on clinical examination in combination with imaging and confirmed by pathological assessment of core needle biopsy (CNB) or fine needle aspiration biopsy (FNAB). The biological profile of the lesion is needed to define the prognosis and establish therapy. Given the importance of an early and minimally invasive diagnosis, we aimed to verify whether the biological features detected in FNAB-derived cytological material reflect the biological characteristics of surgical specimens...
June 26, 2018: Histopathology
Ewen McAlpine, Robert Maritz, Susan Williams
Ethics clearance was obtained from the Human Ethics Research Committee (Medical) of the University of the Witwatersrand. This protocol was approved by the committee on 29/05/2017, with certificate number: M170443. Colour vision deficiency (CVD) is estimated to affect 8% of men and 0.4% of women[1] and one can deduce that CVD affects a percentage of pathologists. This article is protected by copyright. All rights reserved.
June 26, 2018: Histopathology
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