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Akihiko Yoshida, Kaishi Satomi, Makoto Ohno, Yuko Matsushita, Masamichi Takahashi, Yasuji Miyakita, Nobuyoshi Hiraoka, Yoshitaka Narita, Koichi Ichimura
AIMS: Intraoperative frozen section (FS) is an integral component of clinical practice. As a quality control measure, FS diagnosis is subsequently compared with the findings on a FS control slide. These control slides could be used for immunohistochemistry, and the immunohistochemical performance in FS controls is known to be antibody-dependent. Isocitrate dehydrogenase (IDH) is mutated in >80% of lower-grade infiltrating gliomas in adults and ~10% of glioblastomas, with IDH1 (R132H) being the most common...
September 17, 2018: Histopathology
Sanpeng Xu, Yuting Dong, Zitian Huo, Lu Yu, Jin Xue, Guoping Wang, Yaqi Duan
AIMS: SOX11 is known as an essential transcription factor for regulating neurogenesis. Recently, SOX11 has been suggested to be a diagnostic marker and oncogene because of its significant expression in mantle cell lymphoma (MCL). However, SOX11 expression in other tumor types has not been extensively studied yet. MATERIALS AND METHODS: Herein, we examined SOX11 expression in 2026 cases of neuroectodermal, germ cell, mesenchymal, and epithelial tumors by immunohistochemistry...
September 17, 2018: Histopathology
David Cappellen, Charles Balabaud, Paulette Bioulac-Sage
Hepatocellular adenoma with mutations in CTNNB1 gene coding for β-catenin (b-HCA) are subdivided into several groups based on different mutations/deletions in exon 3 (non-S45, and S45) and exon 7 or 8, leading to different levels of β-catenin pathway activation (1). The distinction is clinically relevant because mutation in exon 3 (including S45) is associated with a risk of malignant transformation (2); this risk is absent or very low for mutations in exon 7/8 (1). The glutamine synthetase (GS), a key immunohistochemical marker for the identification of mutations in CTNNB1 gene, presents different staining/pattern depending the mutations...
September 5, 2018: Histopathology
Simone B S P Terra, Aaron S Mansfield, Julie A Vrana, Anja C Roden
Thymic epithelial tumors (TET) are rare malignant neoplasms that have the potential to metastasize, recur, or even have a fatal outcome. While many thymomas have a favorable outcome with a 5-year overall survival of 75.8%, reported 5-year survival rates of thymic carcinomas are only 27.5 to 50.5% [1, 2] Complete resection is the curative treatment of choice, however, high stage TET are often not amenable to complete resection. Therefore, alternate treatments such as anti-PD-1/PD-L1 inhibitors, have been studied in this patient population and remain an area of active investigation...
September 4, 2018: Histopathology
Mieke Van Bockstal, Marcella Baldewijns, Cécile Colpaert, Hélène Dano, Giuseppe Floris, Christine Galant, Kathleen Lambein, Dieter Peeters, Sofie Van Renterghem, Anne-Sophie Van Rompuy, Sofie Verbeke, Stephanie Verschuere, Jo Van Dorpe
AIMS: Robust prognostic markers for ductal carcinoma in situ (DCIS) of the breast require high reproducibility and thus low inter-observer variability. This study compared inter-observer variability among thirteen pathologists, which enabled identification of robust histopathological characteristics. METHODS AND RESULTS: One representative haematoxylin/eosin stained slide was selected for 153 DCIS. All pathologists independently assessed nuclear grade, intraductal calcifications, necrosis, solid growth, stromal changes, stromal inflammation and apocrine differentiation...
August 30, 2018: Histopathology
Mehdi Masoomian, Michelle R Downes, Joan Sweet, Carol Cheung, Andrew Evans, Neil Fleshner, Manjula Maganti, Theodorus Van der Kwast
PURPOSE: Intraductal and cribriform carcinoma of the prostate are increasingly recognized as independent prognosticators of poor outcome both in prostate biopsies and surgical specimens. We studied the concordance of biopsy and prostatectomy diagnosis for these two sub-pathologies, in relationship with pathological stage. MATERIALS AND METHODS: Mandatory synoptic reporting of intraductal and cribriform carcinoma in prostate biopsies and prostatectomy specimens was adopted by two academic institutions in November 2015...
August 30, 2018: Histopathology
Raul S Gonzalez, Justin M M Cates, Kay Washington
AIMS: Colorectal carcinoma (CRC) often has a mucinous component, with more than 50% mucin by volume defining the mucinous subtype of CRC. The prognostic impact of the mucinous phenotype remains unclear. METHODS AND RESULTS: We evaluated 224 CRC with at least 5% mucinous component (herein "mCRC") for patient sex, age, race, and outcome; tumour size, location, stage, and microsatellite instability (MSI) status; percentage of glands producing mucin; percentage of tumour volume composed of mucin; whether tumoural epithelium floated in mucin pools; tumour budding; signet ring cells (SRCs); and peritumoural inflammation (PI)...
August 30, 2018: Histopathology
Yingwei Zhang, Kirk D Jones, Natalia Achtar-Zadeh, Gary Green, Jasleen Kukreja, Biyun Xu, Paul J Wolters
BACKGROUND: The objective of this study was to quantify the impact of pirfenidone or nintedanib treatment on lung histopathology and molecular mediators of fibrosis in patients with idiopathic pulmonary fibrosis (IPF). METHODS: We collected lung tissue from IPF patients at the time of lung transplantation. Histopathologic changes were quantified using a blinded scoring method. Proteins associated with senescence or active TGFβ were quantified in lung tissues by immunoblot and immunostaining...
August 28, 2018: Histopathology
M Gambarotti, A P Dei Tos, D Vanel, P Picci, D Gibertoni, M J Klein, A Righi
AIMS: Osteoblastoma-like osteosarcoma is a rare variant of osteosarcoma (1% of all osteosarcomas), histologically similar to osteoblastoma. In the current WHO classification osteoblastoma-like osteosarcoma is classified within the group of conventional (high-grade) osteosarcomas. However, several published cases have been actually regarded as low-grade malignant tumors. Since strict morphologic criteria to distinguish between low-grade and high-grade lesions are not available we reviewed our series of osteoblastoma-like osteosarcomas in the attempt to identify clinical and morphologic features predictive of aggressiveness...
August 28, 2018: Histopathology
Monique J L Mastboom, Daisy M Hoek, Judith V M G Bovee, Michiel A J van de Sande, Karoly Szuhai
INTRODUCTION: Localized- and diffuse-type tenosynovial giant cell tumours (TGCT) are regarded different clinical and radiological TGCT-types. However, genetically and histopathologically they seem indistinguishable. We aimed to correlate CSF1-expression and CSF1-rearrangement with the biological behaviour of different TGCT-types with clinical outcome (recurrence). METHODS: Along a continuum of extremes, therapy naïve knee TGCT patients with >3 year follow-up, mean age 43(range 6-71)years and 56% female were selected...
August 28, 2018: Histopathology
Kathryn P Scherpelz, Anthony C Wong, Mark W Lingen, Jerome B Taxy, Nicole A Cipriani
CONTEXT: Cervical lymph node metastasis in head and neck squamous cell carcinoma (HNSCC) is common. Preoperative chemoradiotherapy (preCRT) and postoperative chemoradiotherapy (postCRT) is frequently employed in such patients. OBJECTIVES: The prognostic value of viable SCC, treatment effect or no SCC in resected lymph nodes in patients who received or did not receive preCRT and postCRT was investigated. MATERIALS AND METHODS: Resected cervical lymph nodes from 146 patients with HNSCC were evaluated for viable SCC, treatment effect, or no SCC...
August 24, 2018: Histopathology
Margot Gaspard, Laurence Lamant, Emilie Tournier, Thibaud Valentin, Philippe Rochaix, Philippe Terrier, Dominique Ranchere-Vince, Jean-Michel Coindre, Thomas Filleron, Sophie Le Guellec
AIMS: The diagnosis of malignant peripheral nerve sheath tumour (MPNST) may be challenging, especially in the sporadic setting. Owing to the lack of specific histological criteria, immunohistochemical and molecular diagnostic markers, several differential diagnoses must be considered, especially melanoma. Indeed, although S100 protein usually stains melanoma, other melanocytic markers are often negative, especially in spindle cell/desmoplastic type. This pattern of immunoreactivity is like that of some nerve-derived tumours such as MPNST...
August 23, 2018: Histopathology
Julien Dagher, Brett Delahunt, Nathalie Rioux-Leclercq, Lars Egevad, Geoff Coughlin, Nigel Dunglison, Troy Gianduzzo, Boon Kua, Greg Malone, Ben Martin, John Preston, Morgan Pokorny, Simon Wood, Hemamali Samaratunga
BACKGROUND: This study evaluates the impact of tumour associated necrosis (TAN) on metastasis-free survival for clear cell renal cell carcinoma (ccRCC) and determines if TAN provides survival information additional to World Health Organization/International Society of Urological Pathology (WHO/ISUP) grading. DESIGN: The study consisted of 376 cases of ccRCC treated by nephrectomy, for which follow-up was available. WHO/ISUP grade was assigned and sections were assessed for presence of TAN...
August 21, 2018: Histopathology
Bruno Augusto Linhares Almeida Mariz, Ciro Dantas Soares, Maria Goretti Freire de Carvalho, Jacks Jorge-Júnior
BACKGROUND: Fibroblast growth factor-2 (FGF-2) and Fibroblast growth factor receptor-1 (FGFR-1) are associated with tumour invasiveness, cell proliferation, angiogenesis, and metastasis. AIMS: To investigate FGF-2 and FGFR-1 expression in oral epithelial dysplasia (OED) and oral tongue squamous cell carcinoma (OTSCC), and its correlation with OTSCC patients' prognosis. METHODS AND RESULTS: One hundred and sixty-seven cases were retrospectively selected, including 85 surgical specimens of patients with OTSCC, 46 incisional biopsies of OTSCC and 36 OED...
August 21, 2018: Histopathology
Maria Guido, Venancio Af Alves, Charles Balabaud, Prithi S Bathal, Paulette Bioulac-Sage, Romano Colombari, James M Crawford, Amar P Dhillon, Linda D Ferrell, Ryan M Gill, Prodromos Hytiroglou, Yasuni Nakanuma, Valerie Paradis, Alberto Quaglia, Pierre E Rautou, Neil D Theise, Swan Thung, Wilson Ms Tsui, Christine Sempoux, Dale Snover, Dirk J van Leuwen
Idiopathic non-cirrhotic portal hypertension (INCPH) is a rare vascular liver disease that has attained a new interest in recent years. It is characterized by clinical signs of portal hypertension in the absence of cirrhosis or severe fibrosis and any known cause of portal hypertension. Since much uncertainty exists about INCPH pathophysiology and no definite diagnostic tests are available, liver biopsy is an essential tool to achieve a definite diagnosis. Unfortunately, the histological diagnosis of INCPH is not always straightforward, since the characteristic lesions are unevenly distributed, vary greatly in their severity and are often very subtle, and not all are necessarily present in a single case...
August 21, 2018: Histopathology
Eiichi Sasaki, Daisuke Nishikawa, Nobuhiro Hanai, Yasuhisa Hasegawa, Yasushi Yatabe
AIMS: Molecular targeted therapy against EGFR kinase domain mutations has been successfully established for lung cancer. These mutations now have also been reported in head and neck tumours, particularly in inverted sinonasal papillomas (ISPs). The aim of this study was to clarify the spectrum of EGFR mutations in head and neck squamous cell carcinomas and papillomas. METHODS AND RESULTS: We examined EGFR mutations in 288 head and neck squamous cell carcinomas and 58 head and neck papillomas or polyps...
August 17, 2018: Histopathology
Yiang Hui, Shaolei Lu, Hai Wang, Murray B Resnick, Yihong Wang
HER2 overexpression is more frequently observed in ductal carcinoma in situ (DCIS) than in invasive breast carcinoma. This phenomenon is paradoxical to the current understanding of the role of HER2 in the development of aggressive invasive disease. The molecular mechanism instigating the loss of HER2 expression during the progression from in situ to invasive cancer is poorly elucidated. We compared HER2 immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) in 100 breast carcinomas exhibiting 2+ HER2 by IHC with synchronous ipsilateral DCIS...
August 17, 2018: Histopathology
Gyula Pekar, Ioanna Kasselaki, Agnes P Lukacs, Csaba Dekany, Dan Hellberg, Tibor Tot
AIMS: Genetic heterogeneity can pose a challenge to identifying eligible cases for targeted therapy in the human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) 2+ breast carcinoma group. In this study, we characterized this subset of tumors according to clinicopathological parameters. METHODS AND RESULTS: We assessed 1000 tumor cells per case and recorded the number of HER2 and chromosome enumeration probe 17 (CEP17) copies using gene-protein assay slides...
August 16, 2018: Histopathology
Atsuji Matsuyama, Mao Jotatsu, Kazuyoshi Uchihashi, Yojiro Tsuda, Eisuke Shiba, Joji Haratake, Masanori Hisaoka
AIMS: MUC4 is a transmembrane glycoprotein that plays a role in cell growth signalling and is expressed in various epithelial tissues. Gene expression profiling and immunohistochemical analyses revealed that MUC4 is also constantly and specifically expressed in low grade fibromyxoid sarcomas and sclerosing epithelioid fibrosarcomas among the mesenchymal tumours and immunohistochemical detection of MUC4 is extremely useful for their diagnoses. In our routine pathological practice, we noticed that meningiomas are also often positive for MUC4, which has not yet been reported previously, despite the extensive scrutiny of its expression in soft tissue tumours...
August 15, 2018: Histopathology
Liza C Villaruz, Katerina Ancevski Hunter, Brenda F Kurland, Shira Abberbock, Cameron Herbst, Sanja Dacic
AIMS: Several studies have demonstrated analytical comparability between different PD-L1 assays, but their clinical validity in non-small cell lung cancer in terms of response to treatment outside of clinical trials has not been established. The aim of our study is to assess the analytical performance of laboratory developed tests for Ventana SP263 and Agilent/Dako 22C3, and to investigate the association between PD-L1 assays and response to PD-1/L1 inhibitors. METHODS AND RESULTS: PD-L1 SP263 and 22C3 assays were performed on 302 consecutive non-small cell lung carcinoma samples Both assays were optimized for use on the automated Ventana BenchMark ULTRA platform...
August 14, 2018: Histopathology
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