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American Journal of Clinical Pathology

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https://www.readbyqxmd.com/read/28525532/the-effects-of-indirect-and-direct-acting-anticoagulants-on-lupus-anticoagulant-assays-a-large-retrospective-study-at-a-coagulation-reference-laboratory
#1
Jansen N Seheult, Michael P Meyer, Franklin A Bontempo, Irina Chibisov
Objectives: To investigate the effects of indirect- and direct-acting anticoagulants on the interpretation of lupus anticoagulant (LAC) assays. Methods: A retrospective database review was performed to identify all LAC panels from November 2012 to November 2015. The positivity rates for three LAC tests were compared among various anticoagulant medications. Results: This analysis included 7,721 LAC panels. Direct oral anticoagulants, warfarin, and unfractionated heparin (UFH) were associated with higher LAC positivity rates compared with patients not receiving documented anticoagulation (83% for argatroban, 58% for dabigatran, 72% for rivaroxaban, 53% for apixaban, 56% for warfarin, and 36% for UFH vs 29% for no anticoagulation, P < ...
May 19, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28505308/diagnostic-performance-of-ultrasound-guided-fine-needle-aspiration-of-nonpalpable-breast-lesions-in-a-multidisciplinary-setting-the-institut-curie-s-experience
#2
Josep A Farras Roca, Anne Tardivon, Fabienne Thibault, Carl El Khoury, Séverine Alran, Virginie Fourchotte, Véronique Marck, Bernard Alépée, Birigitte Sigal, Yann de Rycke, Roman Rouzier, Jerzy Klijanienko
Objectives: To assess the diagnostic performance of ultrasound-guided fine-needle aspiration (USFNA) in nonpalpable breast lesions (NPBLs) in a multidisciplinary setting. Methods: In total, 2,601 NPBLs underwent USFNA by a radiologist-pathologist team. Gold-standard diagnosis was based on surgery, core-needle biopsy, or 1-year imaging follow-up. USFNA's diagnostic performance was analyzed in different clinical and imaging subgroups. Results: USFNA's sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were, respectively, 92...
May 13, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28505220/multicenter-evaluation-of-the-bruker-maldi-biotyper-ca-system-for-the-identification-of-clinically-important-bacteria-and-yeasts
#3
Deborah A Wilson, Stephen Young, Karen Timm, Susan Novak-Weekley, Elizabeth M Marlowe, Neil Madisen, Jennifer L Lillie, Nathan A Ledeboer, Rebecca Smith, Josh Hyke, Christen Griego-Fullbright, Patricia Jim, Paul A Granato, Matthew L Faron, Joven Cumpio, Blake W Buchan, Gary W Procop
Objectives: A report on the multicenter evaluation of the Bruker MALDI Biotyper CA System (MBT-CA; Bruker Daltonics, Billerica, MA) for the identification of clinically important bacteria and yeasts. Methods: In total, 4,399 isolates of medically important bacteria and yeasts were assessed in the MBT-CA. These included 2,262 aerobic gram-positive (AGP) bacteria, 792 aerobic gram-negative (AGN) bacteria 530 anaerobic (AnA) bacteria, and 815 yeasts (YSTs). Three processing methods were assesed...
May 13, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28498881/can-p40-polyclonal-replace-p63-clone-4a4-in-the-cytologic-diagnosis-of-pulmonary-non-small-cell-carcinoma
#4
Melissa Alexander, Jeanine Chiaffarano, Fang Zhou, Joan Cangiarella, Melissa Yee-Chang, Aylin Simsir
Objectives: Differentiating squamous cell carcinoma from adenocarcinoma (ACA) in cytology specimens can be challenging. Recent literature showed p40 had higher specificity than p63 for this purpose. Methods: We identified 190 cytology cases with p40 (polyclonal) and p63 (monoclonal clone 4A4) immunohistochemistry, including specimens from fine-needle aspirations (FNAs) and effusions. Results: ACAs of lung origin stained for p40 and p63 in 21% and 20% of cases, respectively, regardless of specimen site...
May 12, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28472420/determination-of-vh-family-usage-in-b-cell-malignancies-via-the-biomed-2-igh-pcr-clonality-assay
#5
Thomas J McDonald, Linus Kuo, Frank C Kuo
Objectives: To determine whether V H family usage in B-cell lymphoproliferative disorders can be deduced from polymerase chain reaction (PCR) product-length information obtained through the BIOMED-2 (Invivoscribe, San Diego, CA) clonality assay. Methods: We develop an algorithm that uses the sizing information of the BIOMED-2 immunoglobulin heavy chain (IGH) clonality assay to deduce V H family usage. PCR with family-specific primers on 51 clinical samples containing 54 rearranged alleles were used to validate the algorithm...
May 3, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28472207/a-limited-immunohistochemical-panel-can-subtype-hepatocellular-adenomas-for-routine-practice
#6
Brent K Larson, Maha Guindi
Objectives: β-Catenin-activated hepatocellular adenomas have an elevated risk of harboring foci of hepatocellular carcinoma. Inflammatory adenomas also have an increased propensity for malignant transformation and are associated with a systemic inflammatory syndrome. Patients with these two adenoma subtypes benefit from excision. We assessed whether β-catenin-activated and inflammatory adenomas could be identified using a limited immunohistochemical panel. Methods: Forty-six adenomas were assessed by morphology and β-catenin, serum amyloid A, and glutamine synthetase immunostains...
May 3, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28472350/diagnostic-testing-approaches-for-activated-protein-c-resistance-and-factor-v-leiden-a-comparison-of-institutional-and-national-provider-practices
#7
Juliana Perez Botero, Julie A Majerus, Ann K Strege, Ryan D Johnson, Dong Chen, Rajiv K Pruthi
Objectives: To analyze the economic impact of testing for activated protein C resistance (APC-R) due to factor V Leiden (FVL) mutation with APC-R with reflexive FVL genotyping (algorithmic approach) or genotyping alone. Methods: OptumLabs Data Warehouse (OLDW) data were used to assess testing approaches. Insurance claims for APC-R and FVL in 2013 were compared with the Mayo Clinic database. Centers for Medicare & Medicaid Services diagnostic fee schedules were used to assign costs...
May 2, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28449064/using-the-modified-magee-equation-to-identify-patients-unlikely-to-benefit-from-the-21-gene-recurrence-score-assay-oncotype-dx-assay
#8
Yanjun Hou, Gary Tozbikian, Debra L Zynger, Zaibo Li
Objectives: This study aimed to compare a modified Magee equation with Oncotype DX (Genomic Health, Redwood City, CA) recurrence score (RS) and identify patients who are unlikely to benefit from Oncotype DX. Methods: Magee equation RS was calculated in 438 cases and correlated with Oncotype DX RS. Results: The Pearson correlation coefficient ( r ) for the Magee equation and Oncotype DX RS was 0.6645 ( P  < .00001), and the overall agreement was 66...
April 25, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28419186/cd4-negative-variant-of-cutaneous-blastic-plasmacytoid-dendritic-cell-neoplasm-with-a-novel-pbrm1-mutation-in-an-11-year-old-girl
#9
Nuri Yigit, Luisa Fernanda Suarez, Lisa Giulino Roth, Attilio Orazi, Wayne Tam
Objectives: We report a rare case of CD4- cutaneous blastic plasmacytoid dendritic cell neoplasm (BPDCN) with a novel PBRM1 mutation. Methods: An 11-year-old girl presented with an enlarged mass on her left arm and underwent an incisional biopsy. Results: Histopathologic examination and immunohistochemistry studies showed a monotonous proliferation of blasts that were CD4-, CD56+, and CD123+. There was no evidence of leukemic dissemination...
April 15, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28419183/comparison-of-the-mutational-profiles-of-primary-myelofibrosis-polycythemia-vera-and-essential-thrombocytosis
#10
Jinming Song, Mohammad Hussaini, Hailing Zhang, Haipeng Shao, Dahui Qin, Xiaohui Zhang, Zhenjun Ma, Syeda Mahrukh Hussnain Naqvi, Ling Zhang, Lynn C Moscinski
Objectives: To compare the mutational profiles of patients with primary myelofibrosis (PMF), polycythemia vera (PV), and essential thrombocytosis (ET). Methods: Next-generation sequencing results of 75 cases of PMF, 33 cases of PV, and 27 cases of ET were compared. Results: Mutation rates of ASXL1 and SRSF2 were significantly higher in PMF than in PV or ET. ASXL1 mutations appeared to be more frequently associated with risk of transformation to acute myeloid leukemia than JAK2 or TET2 mutations...
April 15, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28419185/a-new-automated-technology-for-cerebrospinal-fluid-cell-counts-comparison-of-accuracy-and-clinical-impact-of-glocyte-sysmex-xn-and-manual-methods
#11
Linda M Sandhaus, Christine A Dillman, Warren P Hinkle, Julie M MacKenzie, George Hong
Objectives: The purpose of the study was to compare the performance of GloCyte (Advanced Instruments, Norwood, MA), a new semiautomated instrument for cerebrospinal fluid cell counting, with the manual hemocytometer method and the automated Sysmex XN (Sysmex, Kobe, Japan) body fluid mode. The clinical impact of replacing the manual method with either automated method was determined. Methods: Fifty-seven samples from 38 patients were analyzed by all three methods...
April 13, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28398539/bile-cast-nephropathy-in-cirrhotic-patients-effects-of-chronic-hyperbilirubinemia
#12
Michelle Foshat, Heather M Ruff, Wayne G Fischer, Robert E Beach, Mark R Fowler, Hyunsu Ju, Judith F Aronson, Marjan Afrouzian
Objectives: The aim of this study was to determine the prevalence of bile cast nephropathy (BCN) in autopsied cirrhotic patients and to correlate BCN with clinical and laboratory data to direct attention to this underrecognized renal complication of liver failure. Methods: We assessed 114 autopsy cases of cirrhosis for the presence of renal intratubular bile casts using Hall stain for bile. Presence of bile casts was correlated with etiology of cirrhosis, clinical and laboratory data, and histologic findings...
April 7, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28398518/epigenetic-regulation-of-cd133-in-gastrointestinal-stromal-tumors
#13
Helene Geddert, Alexander Braun, Claudia Kayser, Arno Dimmler, Gerhard Faller, Abbas Agaimy, Florian Haller, Evgeny A Moskalev
Objectives: This study ascertained the regulation of the stem cell marker CD133 and its potential applicability for prognostication of gastrointestinal stromal tumors (GISTs). Methods: A total of 95 resected GISTs were included in the study. CD133 protein expression was assessed immunohistochemically on tissue microarrays. Methylation percentage was quantified by pyrosequencing. Gene expression in cell lines GIST48b and GIST882 upon treatment with DNA demethylation agent 5-aza-2'-deoxycytidine was analyzed by quantitative polymerase chain reaction...
April 7, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28398455/systematic-evaluation-of-endothelin-1-measurement-relative-to-traditional-and-modern-biomarkers-for-clinical-assessment-and-prognosis-in-patients-with-chronic-systolic-heart-failure-serial-measurement-and-multimarker-testing
#14
Hanna K Gaggin, Quynh A Truong, Parul U Gandhi, Shweta R Motiwala, Arianna M Belcher, Rory B Weiner, Aaron L Baggish, James L Januzzi
Objectives: To define the role of single or serial measurement of endothelin 1 (ET-1) for prognostication beyond traditional and modern markers of risk in heart failure (HF). Methods: In total, 115 patients with chronic systolic HF were followed for 10 months. Clinical assessment and ET-1, N-terminal pro-B-type natriuretic peptide (NT-proBNP), highly sensitive troponin I (hsTnI), soluble ST2 (sST2), and galectin 3 were measured at each visit. Results: Elevated ET-1 was associated with worse HF, lower right ventricular function, higher pulmonary pressure, and higher left atrial volume index despite similar left ventricular function...
April 7, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28371845/impact-of-consensus-conference-review-on-diagnostic-disagreements-in-the-evaluation-of-cervical-biopsy-specimens
#15
Lester J Layfield, Richard D Hammer, Shellaine R Frazier, Magda Esebua, William W Bivin, Katsiaryna Laziuk, Van T Nguyen, Eric Johannesen, Robert L Schmidt
Objectives: To determine the impact of consensus conferences on the frequency of discrepant cases in a surgical pathology practice. Methods: The percentage of discrepancies in cases reviewed at a weekly consensus conference was calculated for the first and last months of a 13-month period. Both interrater agreement and agreement with the consensus diagnoses were assessed. A total of 309 diagnoses were performed for the first month and 518 for the last month. Both absolute and chance-corrected agreement were calculated for each period...
March 25, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28371875/plasmacytoid-urothelial-carcinoma-of-the-urinary-bladder-a-clinicopathologic-and-immunohistochemical-analysis-of-49-cases
#16
Melanie D Fox, Li Xiao, Miao Zhang, Ashish M Kamat, Arlene Siefker-Radtke, Li Zhang, Colin P Dinney, Bogdan Czerniak, Charles C Guo
Objectives: Plasmacytoid urothelial carcinoma (PUC) of the bladder is a rare histologic variant. We retrospectively analyzed a large series of bladder PUC from a single institution. Methods: The patients consisted of 44 men and five women with a mean age of 62 years (range, 45-86 years). Results: PUC was pure in 23 cases and mixed with other histologic types in 26 cases. All PUCs diffusely invaded the bladder wall. Most PUCs lacked immunoreactivity for the retinoblastoma (RB) gene protein (12/32) and E-cadherin (8/30)...
March 23, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28371931/critical-value-reporting-in-transfusion-medicine-a-survey-of-communication-practices-in-us-facilities
#17
Erika M Reese, Randin C Nelson, Willy A Flegel, Karen M Byrne, Garrett S Booth
Objectives: While critical value procedures have been adopted in most areas of the clinical laboratory, their use in transfusion medicine has not been reviewed in detail. The results of this study present a comprehensive overview of critical value reporting and communication practices in transfusion medicine in the United States. Methods: A web-based survey was developed to collect data on the prevalence of critical value procedures and practices of communicating results...
March 21, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28340228/dual-immunostain-with-satb2-and-ck20-differentiates-appendiceal-mucinous-neoplasms-from-ovarian-mucinous-neoplasms
#18
Zaibo Li, Rachel Roth, Jonathan B Rock, Amy Lehman, William L Marsh, Adrian Suarez, Wendy L Frankel
Objectives: Determination of the primary site of origin for mucinous neoplasms identified in the peritoneal and/or pelvic cavities may be challenging, with major differential diagnoses including appendiceal mucinous neoplasm (AMN) and ovarian mucinous neoplasm (OMN). Special AT-rich sequence binding protein 2 (SATB2) has been shown to be highly selectively expressed in the lower gastrointestinal tract, including the appendix. Methods: We investigated the utility of a dual stain (DS) with SATB2 or caudal type homeobox 2 (CDX2) and cytokeratin 20 (CK20) or villin in distinguishing AMNs from OMNs...
March 18, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28340235/polymerase-chain-reaction-human-papillomavirus-hpv-detection-and-hpv-genotyping-in-invasive-cervical-cancers-with-prior-negative-hc2-test-results
#19
Xiang Tao, Baowen Zheng, Fufen Yin, Zhengyu Zeng, Zaibo Li, Christopher C Griffith, Bing Luo, Xiangdong Ding, Xianrong Zhou, Chengquan Zhao
Objectives: Recently, three large Chinese cohort studies showed that 7.5% to 15.5% of patients with cervical carcinoma had negative high-risk human papillomavirus (hrHPV) test results on prior cytology specimens. These studies raise the question as to whether these negative hrHPV results represent truly hrHPV-negative carcinomas or false-negative hrHPV test results due to limitations in the testing of cytology specimens. This is increasingly an important question with increasing push to use hrHPV testing alone to screen for cervical cancer...
March 15, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28375435/diagnostic-criteria-for-polycythemia-vera-difference-in-changes-between-males-and-females
#20
Richard A Horvitz
No abstract text is available yet for this article.
April 1, 2017: American Journal of Clinical Pathology
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