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Acta Tropica

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https://www.readbyqxmd.com/read/28336270/the-natural-history-of-cystic-echinococcosis-in-untreated-and-albendazole-treated-patients
#1
N Solomon, M Kachani, E Zeyhle, C N L Macpherson
The World Health Organization (WHO) treatment protocols for cystic echinococcosis (CE) are based on the standardized ultrasound (US) classification. This study examined whether the classification reflected the natural history of CE in untreated and albendazole-treated patients. Data were collected during mass US screenings in CE endemic regions among transhumant populations, the Turkana and Berber peoples of Kenya and Morocco. Cysts were classified using the WHO classification. Patient records occurring prior to treatment, and after albendazole administration, were selected...
March 20, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28336269/epidemiology-of-polyparasitism-with-taenia-solium-schistosomes-and-soil-transmitted-helminths-in-the-co-endemic-village-of-malanga-democratic-republic-of-congo
#2
Joule Madinga, Katja Polman, Kirezi Kanobana, Lisette van Lieshout, Eric Brienen, Nicolas Praet, Constantin Kabwe, Sarah Gabriël, Pierre Dorny, Pascal Lutumba, Niko Speybroeck
Helminth co-infections are common in sub-Saharan Africa. However, little is known about the distribution and determinants of co-infections with Taenia solium taeniasis/cysticercosis. Building on a previous community-based study on human cysticercosis in Malanga village, we investigated co-infections with Taenia solium, soil-transmitted helminths (STHs) and Schistosoma spp and associated risk factors in a random subsample of 330 participants. Real time PCR assays were used to detect DNA of soil-transmitted helminths (STHs), T...
March 20, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28336268/malaria-incidence-during-early-childhood-in-rural-burkina-faso-analysis-of-a-birth-cohort-protected-with-insecticide-treated-mosquito-nets
#3
Sabrina Wehner, Gabriele Stieglbauer, Corneille Traoré, Ali Sie, Heiko Becher, Olaf Müller
BACKGROUND: Even in the high transmission areas of sub-Saharan Africa (SSA), the incidence of falciparum malaria varies greatly depending on factors such as age, rainfall pattern, distance to breeding places, quality of houses, and existing vector control measures. Insecticide-treated mosquito nets (ITN) have now become the vector control standard in nearly all of SSA. This study aims to describe and analyse the incidence of malaria in a cohort of young children protected with ITN in rural West Africa...
March 20, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28327413/domestic-dogs-as-reservoir-hosts-for-leishmania-donovani-in-the-southernmost-western-ghats-in-india
#4
P Jambulingam, N Pradeep Kumar, S Nandakumar, K P Paily, R Srinivasan
The peripheral blood samples from domestic dogs (n=47) and wild rats (n=25) in the Kani Tribe settlements, located southernmost part of the Western Ghats, Thiruvananthapuram district, Kerala, India were examined for Leishmania infection. This area is known for cases of leishmaniasis with cutaneous manifestations and sandfly abundance. The tribes domesticate dogs to protect them from untoward activities of wild animals. Leishmania donovani parasite DNA was detected only from 6.4% (n=3) of the blood samples collected from the domestic dogs by amplification of the diagnostic kinetoplast mini-circle DNA and PCR-RFLP analysis of the UTR region of heat shock protein 70 (hsp70) gene...
March 18, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28327412/response-of-inos-and-its-relationship-with-il-22-and-stat3-in-macrophage-activity-in-the-polar-forms-of-leprosy
#5
Jorge Rodrigues de Sousa, Raphael Primo Martins de Sousa, Tinara Leila de Souza Aarão, Leonidas Braga Dias, Francisca Regina Oliveira Carneiro, Juarez Antonio Simões Quaresma
Leprosy is a chronic granulomatous infection that manifests as different clinical forms related to the immunological response. The aim of the study was to evaluated the response of IL-22, STAT3, CD68 and iNOS in leprosy skin lesions. The mean number IL-22 positive cells was 12.12±1.90 cells/field in the TT form and 31.31±2.91 cells/field in the LL form. STAT3 positive cells was 5.29±1.96 cells/field in the TT form, while this number was 11.13±3.48 cells/field in the LL form. The mean number of CD68 positive cells was 25...
March 18, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28322728/phlebotomine-sandflies-of-botswana-a-taxonomic-review-and-a-faunistic-update-with-the-first-record-of-genus-phlebotomus
#6
Andreas Krüger
The first records of phlebotomine sandflies from Botswana have been published only recently, comprising of four species of genus Sergentomyia. This update presents the first record of genus Phlebotomus, namely Ph. (Anaphlebotomus) rodhaini Parrot, which is also the first detection of a putative vector of leishmaniasis in Botswana. In addition, records of the Sergentomyia "bedfordi (Newstead) group" are reviewed, and the molecular taxonomy of all taxa known from Botswana is analysed based on three mitochondrial gene fragments (mtDNA)...
March 18, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28322727/what-is-the-future-of-intestinal-parasitic-diseases-in-developing-countries
#7
LETTER
Elizabeth Brito da Silva Alves, Maria José Conceição, Valmir Laurentino Silva, Ana Beatriz Monteiro Fonseca, Daniela Leles
No abstract text is available yet for this article.
March 16, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28315306/molecular-characterization-of-camelpox-virus-isolates-from-bikaner-india-evidence-of-its-endemicity
#8
Shyam Singh Dahiya, Sachin Kumar, Sharat Chandra Mehta, Raghvendar Singh, Kashi Nath, Shirish D Narnaware, Fateh Chand Tuteja
Camelpox is an important viral disease of camels, which may produce mild skin lesions or severe systemic infections. Camelpox virus (CMLV) isolates retrieved from an incidence of camelpox in camels at Bikaner, India were characterized on the basis of genotype and pathotype. Histopathological examination of the CMLV scab revealed intracytoplasmic-eosinophilic inclusion bodies. The phylogenetic analysis of all eight CMLV isolates for C18L gene nucleotide sequence revealed its clustering with its strains M-96 from Kazakhstan and CMS from Iran...
March 14, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28315305/bionomics-of-anopheles-diptera-culicidae-in-a-malaria-endemic-region-of-sungai-nyamuk-village-sebatik-island-north-kalimantan-indonesia
#9
Sugiarto, Upik Kesumawati Hadi, Susi Soviana, Lukman Hakim
The bionomics of Anopheles was investigated in coastal Sungai Nyamuk Village, Nunukan District, North Kalimantan Province from August 2010 to January 2012. Mosquitoes were captured using human landing collections. A total of 5,103 Anopheles mosquitoes comprising 11 species were caught and 2,259 adult parous females were tested by ELISA for Plasmodium antigen. Anopheles vagus, An. sundaicus and An. subpictus were the most abundant species caught. Overall, Anopheles vagus were zoophilic and exophagic, but there was variation between species...
March 14, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28302528/association-between-enteric-protozoan-parasites-and-gastrointestinal-illness-among-hiv-and-tuberculosis-infected-individuals-in-the-chowke-district-southern-mozambique
#10
María José Irisarri-Gutiérrez, Marta Hernández-de Mingo, Aida de Lucio, Horacio Gil, Lucía Morales, Raimundo Seguí, Edy Nacarapa, Carla Muñoz-Antolí, Fernando Jorge Bornay-Llinares, José Guillermo Esteban, David Carmena
Human immune deficiency virus (HIV) and tuberculosis (TB) infections remain major public health issues globally, particularly in sub-Saharan Africa. Impairment of both cell-mediated and humoral immunity by HIV and/or TB infections may limit the host's defences against other pathogens, including the diarrheagenic protozoan Cryptosporidium spp., Giardia intestinalis, and Entamoeba histolytica. During September-December 2015 a cross-sectional study was conducted to assess the prevalence and molecular diversity of these enteric parasites among HIV- and/or TB-infected patients at a medical reference centre in Chowke district, southern Mozambique...
March 14, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28302529/gene-expression-modulation-of-abc-transporter-genes-in-response-to-permethrin-in-adults-of-the-mosquito-malaria-vector-anopheles-stephensi
#11
Valentina Mastrantonio, Marco Ferrari, Sara Epis, Agata Negri, Giulia Scuccimarra, Matteo Montagna, Guido Favia, Daniele Porretta, Sandra Urbanelli, Claudio Bandi
Living organisms have evolved an array of genes coding for detoxifying enzymes and efflux protein pumps, to cope with endogenous and xenobiotic toxic compounds. The study of the genes activated during toxic exposure is relevant to the area of arthropod vector control, since these genes are one of the targets upon which natural selection acts for the evolution of insecticide resistance. ATP-binding cassette (ABC) transporters participate to insecticide detoxification acting as efflux pumps, that reduce the intracellular concentration of toxic compounds, or of their metabolic derivatives...
March 13, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28300559/expression-purification-and-in-vitro-refolding-of-the-recombinant-truncated-saposin-like-protein-2-antigen-for-development-of-diagnosis-of-human-fascioliasis
#12
Abolfazl Mirzadeh, Zarrintaj Valadkhani, Asiyeh Yoosefy, Jalal Babaie, Majid Golkar, Ahmad Reza Esmaeili Rastaghi, Elham Kazemi-Rad, Keyhan Ashrafi
Early diagnosis of fascioliasis is critical in prevention of injury to the liver and bile ducts. Saposin-like protein (FhSAP-2) is probably the most ideal antigen of Fasciola hepatica for development of ELISA kits. SAP-2 has a conserved tertiary structure containing three disulfide bonds and conformational epitopes. Therefore, antigenicity of SAP-2 is greatly depends on disulfide bond formation and proper folding. We produced the recombinant truncated SAP-2 (rtSAP-2) in the SHuffle® T7 and Rosetta strain of E...
March 11, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28300558/therapeutic-efficacy-of-chloroquine-for-the-treatment-of-plasmodium-vivax-malaria-among-outpatients-at-shawa-robit-health-care-center-north-east-ethiopia
#13
Seble Seifu, Ahmed Zeynudin, Endalew Zemene, Sultan Suleman, Abdissa Biruksew
Nearly 40% of all malaria infection in Ethiopia is caused by Plasmodium vivax (P. vivax). Chloroquine (CQ) is the first line treatment for confirmed P. vivax malaria in the country. However, the efficacy of this drug has been compromised by CQ resistant P. vivax (CRPv) strains. Therefore, the present study was aimed at assessing the therapeutic efficacy of CQ for treatment of P. vivax malaria at Shawa Robit Health Care Centre, North-Ease Ethiopia. A one-arm, 28-day follow-up, in vivo therapeutic efficacy study was conducted from October 2013 to February 2014...
March 11, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28288799/structure-and-kinetics-assays-of-recombinant-schistosoma-mansoni-dihydrofolate-reductase
#14
Vitor Hugo Balasco Serrão, Larissa Romanello, Alexandre Cassago, Juliana Roberta Torini de Souza, Juliana Cheleski, Ricardo DeMarco, José Brandão-Neto, Humberto D'Muniz Pereira
The parasite Schistosoma mansoni possesses all pathways for pyrimidine biosynthesis, in which dihydrofolate reductase (DHFR), thymidylate cycle participants, is essential for nucleotide metabolism to obtain energy and structural nucleic acids. Thus, DHFRs have been widely suggested as therapeutic targets for the treatment of infectious diseases. In this study, we expressed recombinant SmDHFR in a heterologous manner to obtain structural, biochemical and kinetic information. X-ray diffraction of recombinant SmDHFR at 1...
March 11, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28288798/probing-the-efficacy-of-a-heterologous-leishmania-l-viannia-braziliensis-recombinant-enolase-as-a-candidate-vaccine-to-restrict-the-development-of-l-infantum-in-balb-c-mice
#15
Thaís T O Santos, Vívian T Martins, Daniela P Lage, Lourena E Costa, Beatriz C S Salles, Ana M R S Carvalho, Daniel S Dias, Patrícia A F Ribeiro, Miguel A Chávez-Fumagalli, Ricardo A Machado-de-Ávila, Bruno M Roatt, Danielle F de Magalhães-Soares, Daniel Menezes-Souza, Eduardo A F Coelho, Mariana C Duarte
In the present study, the Leishmania braziliensis enolase protein was evaluated as a vaccine candidate against visceral leishmaniasis (VL). The DNA sequence was cloned and the recombinant protein (rEnolase) was evaluated as a vaccine, associated with saponin, as an immune adjuvant. The protective efficacy of the rEnolase plus saponin combination was investigated in BALB/c mice against Leishmania infantum infection. The results revealed that the vaccine induced higher levels of IFN-γ, IL-12, and GM-CSF when a capture ELISA and flow cytometry were performed, as well as an antileishmanial nitrite production after using in vitro stimulation with rEnolase and an antigenic Leishmania preparation...
March 10, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28283442/transdermal-delivery-of-oleanolic-acid-attenuates-pro-inflammatory-cytokine-release-and-ameliorates-anaemia-in-p-berghei-malaria
#16
Happiness Sibiya, Cephas T Musabayane, Musa V Mabandla
Malaria remains a major health problem in many tropical areas. Severe malaria infection is associated with secondary complications including anaemia leading to a need for the search of affordable antimalarial agents that can clear the parasitaemia and ameliorate anaemia during infection. The current study investigated the effects of transdermally delivered OA on malaria parasites, HCT and selected plasma cytokine concentrations in P. berghei-infected male Sprague-Dawley rats. The study was carried out over a period of 21 days, divided into pre-treatment (day 0-7), treatment (day 8-12) and post-treatment (day 13-21) periods...
March 7, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28279703/limitations-to-the-adoption-of-a-standardized-strongyloides-stercoralis-diagnostic-method-case-study-in-the-caribbean
#17
Jennifer K Ketzis
BACKGROUND: Strongyloides stercoralis is frequently under-diagnosed due to the low sensitivity of common faecal diagnostic methods used in clinical laboratories. This leads to a belief that prevalence is low and that S. stercoralis is not an important soil-transmitted helminth (STH). S. stercoralis diagnostic methods with higher sensitivity are available but often not used. Reasons for their lack of use need to be identified and addressed in order to increase awareness of this neglected parasite...
March 6, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28279702/molecular-characterization-of-classical-swine-fever-virus-isolates-from-india-during-2012-14
#18
Elina Khatoon, Nagendra N Barman, Manab Deka, Gitika Rajbongshi, Kongkon Baruah, Nipu Deka, Durlav P Bora, Sachin Kumar
Classical swine fever is a highly contagious and economically important viral disease of pigs. Outbreaks of classical swine fever virus (CSFV) were recorded in different places in the Kamrup district of Assam in India between the years 2012 and 2014. The nucleotide sequences of the 10 CSFV isolates were analyzed based on the partial nucleotide sequences of the E2, 5'NTR and NS5B genes. Phylogenetic analysis indicated the dominance of subgroup 2.2 along with 2.1 strains in the northeast part of India. Variation in the nucleotide sequences of E2, 5'NTR and 3'NS5B genes of CSFV allows tracking changes in the virus population over time...
March 6, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28267441/natural-infection-of-nesokia-indica-with-leishmania-major-and-leishmania-infantum-parasites-in-damghan-city-northern-iran
#19
Behrad Pourmohammadi, Sadegh Mohammadi-Azni, Mohsen Kalantari
Various species of rodents are proven reservoir hosts of zoonotic cutaneous leishmaniasis in different provinces of Iran and potential reservoir hosts of zoonotic visceral leishmaniasis. Therefore, this study was conducted to determine the leishmanial infection of rodents in Damghan city from April to September, 2015. Sum of 100 rodents of three species; Nesokia indica (95), Mus musculus (3), and Microtus socialis (2), were trapped alive and their tissue samples were examined using parasitological and molecular (nested-PCR) methods...
March 4, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28257813/reverse-osmosis-and-ultrafiltration-for-recovery-and-reuse-of-larval-rearing-water-in-anopheles-arabiensis-mass-production-effect-of-water-quality-on-larval-development-and-fitness-of-emerging-adults
#20
Wadaka Mamai, Rebecca Hood-Nowotny, Hamidou Maiga, Adel Barakat Ali, Nanwintoun S Bimbile-Somda, Diloma Dieudonné Soma, Hanano Yamada, Rosemary Susan Lees, Jeremie R L Gilles
BACKGROUND: Countries around the world are showing increased interest in applying the sterile insect technique against mosquito disease vectors. Many countries in which mosquitoes are endemic, and so where vector control using the sterile insect technique may be considered, are located in arid zones where water provision can be costly or unreliable. Water reuse provides an alternate form of water supply. In order to reduce the cost of mass rearing of Anopheles arabiensis mosquitoes, the possibility of recycling and reusing larval rearing water was explored...
March 1, 2017: Acta Tropica
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